>PGBD4|NM_152595|3'UTR
   1 ATACCGATCATCATACACATCTGTTCCATTAGGATTAGAGACAAGTTCTGTTTAGAAATAACTCCAAGATTGGGAGGCCG
  81 ACCTGGGTGGATCACCTGAGA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' guuUGUGG-UAACAG--UGUGAGGu 5' |:||| || || |||| :| Target 5' ---ATACCGATCATCATACACATCt 3'	1 - 22	109.00	-10.60
2	miRNA 3' guUUGUGGUAA--CAGUG-UGAGGu 5' |:: |::|| | |: ||||| Target 5' caAGTTCTGTTTAGAAATAACTCCa 3'	42 - 66	102.00	-9.30
3	miRNA 3' guUUGUGGUAAC--AGUGUGAggu 5' :|| : : || |||| || Target 5' ccGACCTGGGTGGATCAC-CTgag 3'	78 - 100	62.00	-7.20

Mutant ID	Mutant Position	Mutant Source
COSN30481435	13	COSMIC
COSN30459200	80	COSMIC
COSN28566334	105	COSMIC
COSN15625832	424	COSMIC
COSN21114782	445	COSMIC
COSN22332279	577	COSMIC
COSN29724281	776	COSMIC
COSN15625834	825	COSMIC
COSN15625835	1066	COSMIC
COSN26551205	1145	COSMIC
COSN26641663	1664	COSMIC
COSN26642695	1681	COSMIC
COSN32040198	2126	COSMIC
COSN24015108	2134	COSMIC
COSN23846590	2361	COSMIC
COSN28217482	2609	COSMIC
COSN19346276	2694	COSMIC
COSN26143992	2830	COSMIC
COSN29385613	3072	COSMIC
COSN8172724	3383	COSMIC
COSN23507748	3653	COSMIC
COSN29281373	4024	COSMIC
COSN15299763	4025	COSMIC
COSN16160867	4037	COSMIC
COSN29643765	4051	COSMIC
COSN6271168	4259	COSMIC
rs9972552	424	GWAS

Mutant ID	Mutant Position	Mutant Source
rs939045397	2	dbSNP
rs1359419705	4	dbSNP
rs759305834	6	dbSNP
rs772013199	7	dbSNP
rs1455503527	11	dbSNP
rs1396578140	15	dbSNP
rs775396137	17	dbSNP
rs774021041	23	dbSNP
rs1243178979	26	dbSNP
rs1365508696	27	dbSNP
rs760811326	31	dbSNP
rs746323883	34	dbSNP
rs1222736205	41	dbSNP
rs370727329	43	dbSNP
rs374109895	46	dbSNP
rs765463051	47	dbSNP
rs1248891107	51	dbSNP
rs919392475	56	dbSNP
rs545715424	59	dbSNP
rs1049761047	71	dbSNP
rs888063994	77	dbSNP
rs143215981	80	dbSNP
rs769515401	81	dbSNP
rs1354974122	82	dbSNP
rs911643983	84	dbSNP
rs944186139	85	dbSNP
rs1296487831	86	dbSNP
rs1432557546	94	dbSNP
rs1372915169	101	dbSNP
rs976934191	117	dbSNP
rs1388554072	120	dbSNP
rs918774828	130	dbSNP
rs1462623262	132	dbSNP
rs1372709600	135	dbSNP
rs1186929030	144	dbSNP
rs531220148	163	dbSNP
rs1254040391	165	dbSNP
rs1049157064	171	dbSNP
rs549791588	172	dbSNP
rs138143662	181	dbSNP
rs1197018815	185	dbSNP
rs1339493891	186	dbSNP
rs888415282	189	dbSNP
rs997521211	192	dbSNP
rs1225764169	199	dbSNP
rs1349725569	207	dbSNP
rs775071841	208	dbSNP
rs1303850025	212	dbSNP
rs948039574	219	dbSNP
rs1045152748	223	dbSNP
rs1391983120	227	dbSNP
rs906631279	227	dbSNP
rs1395874708	229	dbSNP
rs1166890249	230	dbSNP
rs1004062033	237	dbSNP
rs1395959510	239	dbSNP
rs547995886	244	dbSNP
rs62014728	247	dbSNP
rs1221820557	250	dbSNP
rs1365998221	251	dbSNP
rs892331605	261	dbSNP
rs540108112	264	dbSNP
rs956720492	269	dbSNP
rs1479270264	291	dbSNP
rs1198010467	298	dbSNP
rs1486431394	298	dbSNP
rs1412088430	301	dbSNP
rs1342633530	302	dbSNP
rs1022175305	307	dbSNP
rs1009425412	311	dbSNP
rs1022618547	314	dbSNP
rs953009016	314	dbSNP
rs1473821095	326	dbSNP
rs1007198470	348	dbSNP
rs1376275312	349	dbSNP
rs1018620934	350	dbSNP
rs536570432	350	dbSNP
rs972264481	350	dbSNP
rs568306409	352	dbSNP
rs918756313	352	dbSNP
rs78013925	354	dbSNP
rs1406355852	355	dbSNP
rs558492143	357	dbSNP
rs768354451	358	dbSNP
rs1179369497	359	dbSNP
rs1325975617	359	dbSNP
rs1257694820	362	dbSNP
rs370738215	379	dbSNP
rs375375080	380	dbSNP
rs1286055695	381	dbSNP
rs910156336	387	dbSNP
rs1328379367	395	dbSNP
rs569991998	400	dbSNP
rs1235143992	401	dbSNP
rs1352476219	402	dbSNP
rs1356192893	415	dbSNP
rs62014729	420	dbSNP
rs9972552	424	dbSNP
rs919037059	426	dbSNP
rs939447210	434	dbSNP
rs1319364093	439	dbSNP
rs1457097825	444	dbSNP
rs1349644862	447	dbSNP
rs1228634155	450	dbSNP
rs929452938	452	dbSNP
rs1293700925	453	dbSNP
rs1489688936	460	dbSNP
rs182491941	461	dbSNP
rs909453504	465	dbSNP
rs1221291493	467	dbSNP
rs865990665	468	dbSNP
rs1039269106	483	dbSNP
rs901822082	484	dbSNP
rs1251906818	496	dbSNP
rs1439109266	502	dbSNP
rs767253036	505	dbSNP
rs933340682	511	dbSNP
rs1464278494	512	dbSNP
rs541159348	516	dbSNP
rs1335506390	518	dbSNP
rs1289203413	524	dbSNP
rs1226729664	529	dbSNP
rs1271058171	530	dbSNP
rs1434738778	538	dbSNP
rs891906072	547	dbSNP
rs1043599280	552	dbSNP
rs1165794029	557	dbSNP
rs773166147	558	dbSNP
rs531780306	561	dbSNP
rs1009805036	566	dbSNP
rs1163449692	579	dbSNP
rs760541327	581	dbSNP
rs903698316	584	dbSNP
rs1168854634	585	dbSNP
rs765313304	586	dbSNP
rs1002095010	587	dbSNP
rs1007455252	592	dbSNP
rs1018563669	599	dbSNP
rs1246112747	600	dbSNP
rs1033616229	620	dbSNP
rs1309336802	626	dbSNP
rs553515501	627	dbSNP
rs993228970	632	dbSNP
rs141254131	648	dbSNP
rs1026475206	649	dbSNP
rs1316814196	650	dbSNP
rs984304258	653	dbSNP
rs1357099937	658	dbSNP
rs545654138	661	dbSNP
rs867038997	662	dbSNP
rs1274955048	666	dbSNP
rs969612403	667	dbSNP
rs981061533	671	dbSNP
rs1308219852	672	dbSNP
rs1428672567	672	dbSNP
rs909348238	679	dbSNP
rs758613511	683	dbSNP
rs927980686	697	dbSNP
rs1433625731	705	dbSNP
rs1424293323	709	dbSNP
rs943552383	719	dbSNP
rs564218094	723	dbSNP
rs1481628875	724	dbSNP
rs939358412	729	dbSNP
rs1249223322	731	dbSNP
rs974941790	732	dbSNP
rs113330715	733	dbSNP
rs933309267	740	dbSNP
rs1241669713	749	dbSNP
rs763549648	755	dbSNP
rs543029106	757	dbSNP
rs114357977	758	dbSNP
rs1043527680	766	dbSNP
rs888987471	766	dbSNP
rs1226010527	767	dbSNP
rs1374749430	777	dbSNP
rs1374963699	778	dbSNP
rs1288405819	790	dbSNP
rs528948049	792	dbSNP
rs1368311841	794	dbSNP
rs1040275150	806	dbSNP
rs947463084	811	dbSNP
rs1043549364	814	dbSNP
rs1403010866	826	dbSNP
rs1358383575	830	dbSNP
rs1173815356	834	dbSNP
rs903667038	835	dbSNP
rs1420770008	845	dbSNP
rs564986198	852	dbSNP
rs1002062519	858	dbSNP
rs1471656150	862	dbSNP
rs1033583661	863	dbSNP
rs901469555	869	dbSNP
rs896439271	874	dbSNP
rs1013667922	889	dbSNP
rs1483106585	890	dbSNP
rs1380282079	901	dbSNP
rs150757872	911	dbSNP
rs1298085227	912	dbSNP
rs1318035011	912	dbSNP
rs1288342455	914	dbSNP
rs950441668	920	dbSNP
rs1026032998	923	dbSNP
rs1250261832	941	dbSNP
rs1345181210	944	dbSNP
rs548409461	947	dbSNP
rs1396277886	969	dbSNP
rs764445075	974	dbSNP
rs566230231	979	dbSNP
rs1261858643	985	dbSNP
rs1300838560	986	dbSNP
rs964829499	991	dbSNP
rs1389207713	993	dbSNP
rs1158356342	1000	dbSNP
rs1455667994	1013	dbSNP
rs974909234	1015	dbSNP
rs1188384301	1017	dbSNP
rs923515910	1023	dbSNP
rs1005995430	1026	dbSNP
rs1189559542	1035	dbSNP
rs1017113984	1045	dbSNP
rs1264316749	1049	dbSNP
rs367740043	1067	dbSNP
rs1207413982	1071	dbSNP
rs1192832488	1075	dbSNP
rs1266662655	1079	dbSNP
rs186818346	1080	dbSNP
rs1481490369	1084	dbSNP
rs1174403812	1088	dbSNP
rs1403748607	1089	dbSNP
rs1383650543	1092	dbSNP
rs1035686052	1095	dbSNP
rs913205441	1096	dbSNP
rs947396443	1117	dbSNP
rs781653247	1118	dbSNP
rs55816157	1119	dbSNP
rs1163897076	1131	dbSNP
rs1457528986	1132	dbSNP
rs1043130223	1142	dbSNP
rs1366868584	1147	dbSNP
rs927392385	1147	dbSNP
rs937819791	1167	dbSNP
rs1450423547	1173	dbSNP
rs1247830562	1175	dbSNP
rs960718062	1179	dbSNP
rs552043939	1187	dbSNP
rs192395576	1188	dbSNP
rs946799574	1189	dbSNP
rs1224771816	1198	dbSNP
rs1355058301	1202	dbSNP
rs979520132	1203	dbSNP
rs1013469212	1205	dbSNP
rs1048119841	1219	dbSNP
rs1230203299	1225	dbSNP
rs1280838046	1225	dbSNP
rs1296571320	1229	dbSNP
rs1323381184	1232	dbSNP
rs1209913864	1235	dbSNP
rs943207344	1237	dbSNP
rs373658769	1239	dbSNP
rs537774277	1240	dbSNP
rs576982772	1253	dbSNP
rs1204754341	1258	dbSNP
rs371780346	1259	dbSNP
rs1389832628	1276	dbSNP
rs1015951965	1292	dbSNP
rs1167081725	1297	dbSNP
rs1455902194	1300	dbSNP
rs901769262	1303	dbSNP
rs1378664031	1306	dbSNP
rs1198810022	1307	dbSNP
rs1450500305	1308	dbSNP
rs1197287995	1315	dbSNP
rs746379071	1324	dbSNP
rs114785009	1325	dbSNP
rs1483172425	1327	dbSNP
rs996297363	1334	dbSNP
rs567665206	1348	dbSNP
rs1030415949	1349	dbSNP
rs779780005	1354	dbSNP
rs955060797	1355	dbSNP
rs887492567	1356	dbSNP
rs1365300682	1360	dbSNP
rs1290264245	1362	dbSNP
rs1005969117	1367	dbSNP
rs989055928	1370	dbSNP
rs913501048	1374	dbSNP
rs1183710318	1377	dbSNP
rs1306405381	1377	dbSNP
rs1371008326	1377	dbSNP
rs147210066	1377	dbSNP
rs398057585	1377	dbSNP
rs543545054	1377	dbSNP
rs559589772	1377	dbSNP
rs56874167	1377	dbSNP
rs572389275	1377	dbSNP
rs1365760782	1378	dbSNP
rs199660470	1379	dbSNP
rs867084651	1383	dbSNP
rs1472636105	1388	dbSNP
rs66517123	1393	dbSNP
rs968737112	1395	dbSNP
rs979205754	1397	dbSNP
rs1491180744	1398	dbSNP
rs927359642	1398	dbSNP
rs56834847	1399	dbSNP
rs905323341	1410	dbSNP
rs1443887415	1425	dbSNP
rs937371493	1426	dbSNP
rs748803722	1428	dbSNP
rs1406408434	1439	dbSNP
rs1279030321	1440	dbSNP
rs1199707392	1450	dbSNP
rs1342766750	1456	dbSNP
rs1270007585	1462	dbSNP
rs1002392818	1463	dbSNP
rs768246556	1464	dbSNP
rs547115024	1469	dbSNP
rs1286643158	1475	dbSNP
rs553061942	1479	dbSNP
rs988435097	1491	dbSNP
rs1344396310	1497	dbSNP
rs1333908566	1500	dbSNP
rs886382992	1511	dbSNP
rs115499630	1523	dbSNP
rs1037366066	1526	dbSNP
rs1391386555	1551	dbSNP
rs1160570600	1554	dbSNP
rs1309852636	1567	dbSNP
rs1315476702	1569	dbSNP
rs1238714550	1577	dbSNP
rs771859981	1579	dbSNP
rs1361773800	1586	dbSNP
rs968028839	1588	dbSNP
rs1473561984	1589	dbSNP
rs1268249097	1591	dbSNP
rs979445500	1593	dbSNP
rs1440485549	1601	dbSNP
rs1249746464	1604	dbSNP
rs910653321	1604	dbSNP
rs995846735	1605	dbSNP
rs1328445058	1617	dbSNP
rs1177682107	1623	dbSNP
rs1030057714	1626	dbSNP
rs890612301	1631	dbSNP
rs1266296295	1638	dbSNP
rs541028745	1640	dbSNP
rs923151362	1644	dbSNP
rs929209586	1648	dbSNP
rs1020528443	1653	dbSNP
rs1158030345	1665	dbSNP
rs1047676475	1671	dbSNP
rs1385640310	1672	dbSNP
rs760661787	1675	dbSNP
rs1321762173	1676	dbSNP
rs79257844	1680	dbSNP
rs576242368	1683	dbSNP
rs941680981	1686	dbSNP
rs1364386357	1698	dbSNP
rs1442357134	1699	dbSNP
rs1044146418	1706	dbSNP
rs905625784	1713	dbSNP
rs1442507068	1715	dbSNP
rs753533527	1725	dbSNP
rs1299418745	1729	dbSNP
rs1321918610	1731	dbSNP
rs1371734207	1734	dbSNP
rs1002653094	1741	dbSNP
rs867144033	1742	dbSNP
rs1316162224	1747	dbSNP
rs1452271175	1750	dbSNP
rs139068414	1753	dbSNP
rs1344627960	1754	dbSNP
rs958707107	1757	dbSNP
rs561499525	1766	dbSNP
rs1363632027	1772	dbSNP
rs1295802229	1785	dbSNP
rs917345541	1786	dbSNP
rs1349981383	1794	dbSNP
rs149881179	1797	dbSNP
rs1391699800	1798	dbSNP
rs1168989059	1802	dbSNP
rs1448649667	1803	dbSNP
rs754572996	1806	dbSNP
rs541086101	1821	dbSNP
rs1206655210	1825	dbSNP
rs983722603	1835	dbSNP
rs967996727	1846	dbSNP
rs1246168372	1849	dbSNP
rs1000833630	1863	dbSNP
rs559480986	1866	dbSNP
rs147554542	1867	dbSNP
rs976229229	1870	dbSNP
rs1204797533	1872	dbSNP
rs1340493898	1873	dbSNP
rs551658196	1877	dbSNP
rs563938962	1882	dbSNP
rs1446277017	1883	dbSNP
rs1181341472	1886	dbSNP
rs1402636733	1903	dbSNP
rs1371953263	1905	dbSNP
rs140272932	1908	dbSNP
rs931674621	1910	dbSNP
rs763066087	1911	dbSNP
rs7182085	1919	dbSNP
rs909149001	1926	dbSNP
rs1197356258	1941	dbSNP
rs941649607	1954	dbSNP
rs548445127	1955	dbSNP
rs147954291	1956	dbSNP
rs1181754627	1958	dbSNP
rs1479837891	1969	dbSNP
rs1258116883	1973	dbSNP
rs1211340436	1974	dbSNP
rs530635991	1988	dbSNP
rs551926816	1989	dbSNP
rs1020832281	1996	dbSNP
rs904698922	1997	dbSNP
rs1351823550	2002	dbSNP
rs1450859328	2003	dbSNP
rs1408803802	2004	dbSNP
rs926969678	2010	dbSNP
rs1000421144	2013	dbSNP
rs534691210	2014	dbSNP
rs1358435092	2018	dbSNP
rs1056890051	2023	dbSNP
rs958676309	2030	dbSNP
rs1458841670	2035	dbSNP
rs1412830247	2036	dbSNP
rs570615423	2037	dbSNP
rs1258616963	2039	dbSNP
rs992857054	2044	dbSNP
rs1024384914	2045	dbSNP
rs1197913822	2046	dbSNP
rs1182741920	2050	dbSNP
rs896607411	2052	dbSNP
rs1009754918	2067	dbSNP
rs546639489	2071	dbSNP
rs1330476957	2072	dbSNP
rs1268180826	2077	dbSNP
rs951584208	2078	dbSNP
rs1199196170	2082	dbSNP
rs757484158	2096	dbSNP
rs183460810	2101	dbSNP
rs1378196500	2102	dbSNP
rs904099055	2104	dbSNP
rs1201492135	2120	dbSNP
rs1001094936	2121	dbSNP
rs907665260	2125	dbSNP
rs1187954882	2126	dbSNP
rs1416741728	2130	dbSNP
rs1427447750	2133	dbSNP
rs1176560502	2142	dbSNP
rs1408876099	2153	dbSNP
rs1468426593	2154	dbSNP
rs1165778618	2170	dbSNP
rs1423560494	2182	dbSNP
rs1176372093	2189	dbSNP
rs1017554708	2201	dbSNP
rs1468712700	2207	dbSNP
rs1336409868	2225	dbSNP
rs941880241	2228	dbSNP
rs964712604	2231	dbSNP
rs1203969147	2234	dbSNP
rs1484612450	2236	dbSNP
rs997977690	2249	dbSNP
rs187435996	2250	dbSNP
rs921883599	2251	dbSNP
rs931643709	2262	dbSNP
rs1312631127	2263	dbSNP
rs1230427002	2270	dbSNP
rs1319828969	2275	dbSNP
rs983319089	2282	dbSNP
rs530903093	2291	dbSNP
rs879699356	2291	dbSNP
rs140586174	2292	dbSNP
rs1433138900	2292	dbSNP
rs1457576926	2298	dbSNP
rs1268691033	2301	dbSNP
rs1051908604	2310	dbSNP
rs1361325028	2313	dbSNP
rs909075820	2313	dbSNP
rs1450484492	2320	dbSNP
rs1390036937	2321	dbSNP
rs890131329	2324	dbSNP
rs1268798314	2336	dbSNP
rs762243698	2338	dbSNP
rs1209962611	2339	dbSNP
rs945752592	2341	dbSNP
rs534754631	2360	dbSNP
rs71119932	2360	dbSNP
rs796797907	2362	dbSNP
rs557635141	2366	dbSNP
rs1225116477	2371	dbSNP
rs547309079	2371	dbSNP
rs904666755	2374	dbSNP
rs1000388264	2377	dbSNP
rs376919668	2379	dbSNP
rs576208698	2379	dbSNP
rs113896496	2389	dbSNP
rs1239963293	2396	dbSNP
rs1430743466	2398	dbSNP
rs894762848	2399	dbSNP
rs74956322	2403	dbSNP
rs1468006773	2410	dbSNP
rs1378789056	2416	dbSNP
rs1198874515	2423	dbSNP
rs28376576	2429	dbSNP
rs1183938604	2434	dbSNP
rs1479740905	2435	dbSNP
rs951420183	2444	dbSNP
rs1483202468	2445	dbSNP
rs1004674848	2452	dbSNP
rs1193920585	2461	dbSNP
rs936914800	2462	dbSNP
rs1017044176	2464	dbSNP
rs78841228	2468	dbSNP
rs1256520398	2471	dbSNP
rs750816209	2472	dbSNP
rs963170400	2479	dbSNP
rs1343745923	2483	dbSNP
rs1176410728	2486	dbSNP
rs756728781	2503	dbSNP
rs1410629216	2511	dbSNP
rs1335445184	2517	dbSNP
rs1402850440	2524	dbSNP
rs900464833	2531	dbSNP
rs1403466425	2536	dbSNP
rs997546866	2537	dbSNP
rs1030805847	2543	dbSNP
rs1426551346	2544	dbSNP
rs541022549	2545	dbSNP
rs777628307	2546	dbSNP
rs1405801077	2551	dbSNP
rs921851667	2555	dbSNP
rs754250414	2560	dbSNP
rs559415772	2568	dbSNP
rs963241706	2570	dbSNP
rs577796470	2579	dbSNP
rs754547986	2582	dbSNP
rs1270067323	2583	dbSNP
rs911520239	2587	dbSNP
rs1274222797	2601	dbSNP
rs1278019396	2605	dbSNP
rs1351349266	2626	dbSNP
rs1225210307	2627	dbSNP
rs1341477820	2634	dbSNP
rs1281901371	2635	dbSNP
rs1451073386	2640	dbSNP
rs1391547985	2643	dbSNP
rs550001506	2646	dbSNP
rs1400785150	2647	dbSNP
rs1406711572	2652	dbSNP
rs1352440213	2660	dbSNP
rs1156464537	2661	dbSNP
rs545103120	2662	dbSNP
rs1222856613	2664	dbSNP
rs1270397810	2668	dbSNP
rs1473630881	2668	dbSNP
rs1182808966	2669	dbSNP
rs1209376204	2669	dbSNP
rs1230210336	2669	dbSNP
rs1314783951	2669	dbSNP
rs1357389330	2669	dbSNP
rs1368796537	2669	dbSNP
rs1446524774	2669	dbSNP
rs60502890	2669	dbSNP
rs68176283	2669	dbSNP
rs1489667026	2671	dbSNP
rs1244324692	2678	dbSNP
rs945751853	2687	dbSNP
rs1367890317	2688	dbSNP
rs1299007859	2689	dbSNP
rs1430755974	2690	dbSNP
rs1198741442	2691	dbSNP
rs1344118703	2691	dbSNP
rs148155436	2693	dbSNP
rs925802373	2694	dbSNP
rs1471456283	2695	dbSNP
rs35445881	2695	dbSNP
rs202073733	2696	dbSNP
rs1407793776	2700	dbSNP
rs1365871695	2702	dbSNP
rs1425517353	2703	dbSNP
rs868627256	2703	dbSNP
rs866110312	2704	dbSNP
rs925720409	2709	dbSNP
rs936145934	2712	dbSNP
rs1189178141	2714	dbSNP
rs568439215	2715	dbSNP
rs1475066680	2716	dbSNP
rs1056013339	2719	dbSNP
rs1223947871	2721	dbSNP
rs116316084	2724	dbSNP
rs1291326997	2725	dbSNP
rs959944014	2727	dbSNP
rs1336216158	2729	dbSNP
rs1014478336	2735	dbSNP
rs1444298040	2737	dbSNP
rs34044919	2756	dbSNP
rs531146213	2756	dbSNP
rs992492893	2757	dbSNP
rs1360549353	2760	dbSNP
rs549353559	2767	dbSNP
rs1375952843	2775	dbSNP
rs887081926	2781	dbSNP
rs1422473990	2787	dbSNP
rs184844431	2787	dbSNP
rs1372145140	2797	dbSNP
rs538189263	2799	dbSNP
rs1170946165	2809	dbSNP
rs1356868176	2812	dbSNP
rs1330174880	2814	dbSNP
rs528788700	2819	dbSNP
rs1268384702	2821	dbSNP
rs1197572140	2826	dbSNP
rs371960458	2836	dbSNP
rs1484179881	2837	dbSNP
rs1275770089	2840	dbSNP
rs1204336328	2842	dbSNP
rs1306263684	2843	dbSNP
rs1281351847	2862	dbSNP
rs1220407985	2865	dbSNP
rs778221693	2866	dbSNP
rs1282135439	2873	dbSNP
rs747852590	2875	dbSNP
rs1298532743	2880	dbSNP
rs962808716	2881	dbSNP
rs1352635058	2884	dbSNP
rs1178165969	2885	dbSNP
rs1273117242	2888	dbSNP
rs1428642334	2890	dbSNP
rs1439999183	2893	dbSNP
rs936846428	2899	dbSNP
rs11072947	2903	dbSNP
rs1028750180	2905	dbSNP
rs900768103	2911	dbSNP
rs1479869609	2914	dbSNP
rs953227242	2936	dbSNP
rs987771729	2938	dbSNP
rs911838019	2945	dbSNP
rs571325228	2959	dbSNP
rs886479396	2966	dbSNP
rs1004963392	2972	dbSNP
rs1330697157	2973	dbSNP
rs1307283551	2976	dbSNP
rs535799782	2983	dbSNP
rs977148703	2984	dbSNP
rs1304411264	2985	dbSNP
rs1453253790	2994	dbSNP
rs925689531	3000	dbSNP
rs935694474	3003	dbSNP
rs898975078	3008	dbSNP
rs1055559337	3012	dbSNP
rs1364540557	3017	dbSNP
rs201033050	3020	dbSNP
rs1158694547	3023	dbSNP
rs538721243	3025	dbSNP
rs1416382156	3029	dbSNP
rs1411578501	3032	dbSNP
rs1034624607	3037	dbSNP
rs959964571	3041	dbSNP
rs950263518	3044	dbSNP
rs1046393724	3047	dbSNP
rs1486576480	3049	dbSNP
rs1260903797	3055	dbSNP
rs148992518	3057	dbSNP
rs940015108	3058	dbSNP
rs1314589406	3060	dbSNP
rs1246525938	3076	dbSNP
rs978444528	3082	dbSNP
rs1312847631	3084	dbSNP
rs1038296900	3085	dbSNP
rs1385723659	3093	dbSNP
rs898509430	3096	dbSNP
rs1316932149	3097	dbSNP
rs996858644	3098	dbSNP
rs146555053	3112	dbSNP
rs368948481	3112	dbSNP
rs1028383847	3115	dbSNP
rs569709285	3128	dbSNP
rs1243624170	3136	dbSNP
rs569536410	3143	dbSNP
rs1383878869	3144	dbSNP
rs1008758939	3153	dbSNP
rs1429667150	3153	dbSNP
rs1261784658	3159	dbSNP
rs907837109	3170	dbSNP
rs1019270297	3176	dbSNP
rs540058832	3185	dbSNP
rs1222875484	3187	dbSNP
rs756524368	3188	dbSNP
rs1257926140	3191	dbSNP
rs1462181165	3197	dbSNP
rs977744104	3200	dbSNP
rs1205899587	3201	dbSNP
rs746774387	3206	dbSNP
rs1229493775	3212	dbSNP
rs1423450307	3215	dbSNP
rs1001641932	3228	dbSNP
rs537239301	3231	dbSNP
rs957037419	3236	dbSNP
rs1369933970	3239	dbSNP
rs1304006588	3243	dbSNP
rs1474495320	3246	dbSNP
rs1424382584	3247	dbSNP
rs143737363	3248	dbSNP
rs1364068046	3250	dbSNP
rs558333598	3256	dbSNP
rs577019570	3259	dbSNP
rs915686254	3259	dbSNP
rs1169051235	3262	dbSNP
rs1399403084	3267	dbSNP
rs770903487	3273	dbSNP
rs1490098951	3280	dbSNP
rs1266494694	3296	dbSNP
rs1020621249	3300	dbSNP
rs12440908	3308	dbSNP
rs1250708103	3323	dbSNP
rs776649292	3325	dbSNP
rs966996951	3329	dbSNP
rs981674497	3334	dbSNP
rs573958202	3349	dbSNP
rs908708253	3350	dbSNP
rs1343931514	3353	dbSNP
rs541127054	3354	dbSNP
rs559282907	3357	dbSNP
rs1331280662	3365	dbSNP
rs1392334479	3367	dbSNP
rs1396332248	3367	dbSNP
rs963748052	3367	dbSNP
rs1459989022	3370	dbSNP
rs1224565089	3373	dbSNP
rs759348888	3382	dbSNP
rs1479903706	3384	dbSNP
rs1218656449	3397	dbSNP
rs975608271	3411	dbSNP
rs1183650159	3427	dbSNP
rs189780765	3429	dbSNP
rs552969117	3432	dbSNP
rs1324828015	3434	dbSNP
rs577735288	3435	dbSNP
rs1261655340	3436	dbSNP
rs1483151290	3440	dbSNP
rs898478352	3442	dbSNP
rs1223048974	3448	dbSNP
rs1264062745	3452	dbSNP
rs932678510	3455	dbSNP
rs1445525062	3459	dbSNP
rs1049788258	3460	dbSNP
rs1430084996	3461	dbSNP
rs1327314377	3467	dbSNP
rs1480712783	3470	dbSNP
rs1178577280	3471	dbSNP
rs1375703950	3475	dbSNP
rs544729727	3476	dbSNP
rs1009143970	3477	dbSNP
rs1159974746	3482	dbSNP
rs1018741668	3488	dbSNP
rs1470333031	3490	dbSNP
rs1386598440	3492	dbSNP
rs151065163	3493	dbSNP
rs903021957	3494	dbSNP
rs575293909	3496	dbSNP
rs376067034	3498	dbSNP
rs1345668454	3499	dbSNP
rs908133319	3502	dbSNP
rs1032945245	3511	dbSNP
rs1436497130	3514	dbSNP
rs940949557	3523	dbSNP
rs1350307092	3526	dbSNP
rs1278144013	3532	dbSNP
rs1248633481	3537	dbSNP
rs1384107940	3542	dbSNP
rs1314998008	3560	dbSNP
rs957006917	3575	dbSNP
rs920632318	3576	dbSNP
rs1281984655	3577	dbSNP
rs1319778784	3583	dbSNP
rs991189031	3589	dbSNP
rs7167335	3590	dbSNP
rs561180275	3593	dbSNP
rs971133772	3594	dbSNP
rs981608405	3604	dbSNP
rs528729404	3617	dbSNP
rs1182943307	3619	dbSNP
rs1269685086	3625	dbSNP
rs1014524516	3626	dbSNP
rs940201973	3627	dbSNP
rs554831299	3629	dbSNP
rs1041608709	3642	dbSNP
rs1490876228	3644	dbSNP
rs1197394145	3647	dbSNP
rs1282441018	3649	dbSNP
rs192918138	3653	dbSNP
rs564890704	3654	dbSNP
rs1000094876	3656	dbSNP
rs1432530763	3665	dbSNP
rs1364776130	3669	dbSNP
rs920207596	3671	dbSNP
rs1271423176	3681	dbSNP
rs1430823956	3684	dbSNP
rs1325427694	3688	dbSNP
rs574360847	3693	dbSNP
rs762194573	3694	dbSNP
rs1406499265	3697	dbSNP
rs1365928049	3698	dbSNP
rs1365381758	3702	dbSNP
rs1417479545	3704	dbSNP
rs1049755001	3706	dbSNP
rs1162747796	3706	dbSNP
rs1157719755	3709	dbSNP
rs1365636037	3712	dbSNP
rs1425952872	3713	dbSNP
rs912555657	3716	dbSNP
rs574706300	3719	dbSNP
rs767819934	3720	dbSNP
rs996575260	3724	dbSNP
rs1168512766	3737	dbSNP
rs944102823	3738	dbSNP
rs1240537163	3748	dbSNP
rs1042877107	3764	dbSNP
rs1029829050	3781	dbSNP
rs750871369	3817	dbSNP
rs761147823	3819	dbSNP
rs1268615428	3823	dbSNP
rs532548960	3829	dbSNP
rs183489272	3831	dbSNP
rs893099778	3834	dbSNP
rs1292719215	3835	dbSNP
rs1361908753	3843	dbSNP
rs1220267181	3850	dbSNP
rs1264953835	3855	dbSNP
rs569329137	3859	dbSNP
rs1177778611	3862	dbSNP
rs1252367285	3863	dbSNP
rs150199302	3864	dbSNP
rs1015123412	3865	dbSNP
rs1177796653	3866	dbSNP
rs962285462	3867	dbSNP
rs1446084442	3871	dbSNP
rs1162017617	3872	dbSNP
rs1329497558	3883	dbSNP
rs199856169	3887	dbSNP
rs1421549808	3890	dbSNP
rs766613821	3895	dbSNP
rs973682762	3897	dbSNP
rs188218114	3902	dbSNP
rs1189779146	3903	dbSNP
rs937325628	3909	dbSNP
rs1462938214	3911	dbSNP
rs1233922979	3913	dbSNP
rs181796595	3916	dbSNP
rs112535526	3917	dbSNP
rs534887537	3918	dbSNP
rs961556005	3920	dbSNP
rs1224889078	3926	dbSNP
rs1323727345	3930	dbSNP
rs778545414	3954	dbSNP
rs553306163	3955	dbSNP
rs533810691	3957	dbSNP
rs1217224340	3959	dbSNP
rs1375219584	3960	dbSNP
rs1279975580	3961	dbSNP
rs920174721	3966	dbSNP
rs1316179811	3970	dbSNP
rs1041924823	3972	dbSNP
rs571221231	3977	dbSNP
rs1398113886	3979	dbSNP
rs1338700016	3982	dbSNP
rs1470349740	3983	dbSNP
rs1405557765	4000	dbSNP
rs903066525	4001	dbSNP
rs1421148470	4004	dbSNP
rs538626968	4005	dbSNP
rs1177048030	4008	dbSNP
rs752142121	4015	dbSNP
rs935913268	4033	dbSNP
rs554043679	4037	dbSNP
rs556710694	4038	dbSNP
rs954255818	4043	dbSNP
rs1054309920	4052	dbSNP
rs770103764	4054	dbSNP
rs1254946669	4070	dbSNP
rs775930129	4075	dbSNP
rs1207562328	4083	dbSNP
rs985684278	4087	dbSNP
rs1226879736	4090	dbSNP
rs1307440791	4094	dbSNP
rs575282845	4114	dbSNP
rs1374492194	4124	dbSNP
rs758085124	4134	dbSNP
rs1453412938	4139	dbSNP
rs1308196875	4140	dbSNP
rs1301217099	4142	dbSNP
rs1205110045	4144	dbSNP
rs1455527745	4154	dbSNP
rs1252950676	4156	dbSNP
rs1158749233	4157	dbSNP
rs542376369	4160	dbSNP
rs944053030	4165	dbSNP
rs763173602	4167	dbSNP
rs1444617346	4172	dbSNP
rs924048110	4173	dbSNP
rs563623395	4181	dbSNP
rs1486608182	4185	dbSNP
rs1054252411	4188	dbSNP
rs115398043	4195	dbSNP
rs4780214	4196	dbSNP
rs962209866	4200	dbSNP
rs555825939	4202	dbSNP
rs1358712568	4203	dbSNP
rs1310655718	4210	dbSNP
rs1422030923	4211	dbSNP
rs1465576481	4226	dbSNP
rs141942299	4227	dbSNP
rs1288543688	4230	dbSNP
rs1398430337	4234	dbSNP
rs1027925398	4235	dbSNP
rs565227610	4259	dbSNP
rs780780381	4288	dbSNP
rs1446051660	4289	dbSNP
rs958707314	4290	dbSNP
rs1429793660	4291	dbSNP
rs1261943984	4292	dbSNP
rs1192438187	4304	dbSNP
rs1362945932	4305	dbSNP
rs1487131072	4320	dbSNP
rs991887317	4322	dbSNP
rs116558118	4330	dbSNP
rs1488078372	4331	dbSNP
rs1263625893	4333	dbSNP
rs1229652013	4342	dbSNP
rs1308121529	4362	dbSNP
rs1276388016	4366	dbSNP
rs950177596	4370	dbSNP
rs762281351	4371	dbSNP
rs1347696443	4375	dbSNP
rs1304065600	4379	dbSNP
rs1404738889	4386	dbSNP
rs1015336792	4387	dbSNP

- Genes & development, 2013

When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.