ID	Duplex structure	Position	Score	MFE
1	miRNA 3' gucgaccaACUUC-CCCUGGUUu 5' ||:|| ||:|:|| Target 5' gtaccccaTGGAGCTGGGCTAAg 3'	790 - 812	118.00	-12.20
2	miRNA 3' guCGACCAAC--UUCCCCUGGUUu 5' |:| ||| || ||| :||| Target 5' caGTTTTTTGAAAATGGGCTCAAc 3'	444 - 467	114.00	-11.90
3	miRNA 3' gucgaccaacuucccCUGGUUu 5' |||:|| Target 5' ctcttctctccagctGACTAAa 3'	983 - 1004	104.00	-8.60

Mutant ID	Mutant Position	Mutant Source
COSN24299673	19	COSMIC
COSN20057406	29	COSMIC
COSN30172110	39	COSMIC
COSN30552363	57	COSMIC
COSN31532839	76	COSMIC
COSN30487337	83	COSMIC
COSN31520653	107	COSMIC
COSN31489226	122	COSMIC
COSN30186939	135	COSMIC
COSN22118720	151	COSMIC
COSN30124256	169	COSMIC
COSN31544666	174	COSMIC
COSN31543321	189	COSMIC
COSN7699856	258	COSMIC
COSN31555430	265	COSMIC
COSN31547017	273	COSMIC
COSN1951682	600	COSMIC
COSN25942207	635	COSMIC
COSN31530037	663	COSMIC
COSN30173990	689	COSMIC
COSN31536439	739	COSMIC
COSN31513937	740	COSMIC
COSN31593808	830	COSMIC
COSN30174021	876	COSMIC
COSN32056571	890	COSMIC
COSN24924808	1041	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs372122347	3	dbSNP
rs1450535254	4	dbSNP
rs1392517672	5	dbSNP
rs1182322411	7	dbSNP
rs1481832152	10	dbSNP
rs766538557	13	dbSNP
rs188509642	15	dbSNP
rs758614674	18	dbSNP
rs750801629	20	dbSNP
rs1268965726	22	dbSNP
rs368143471	26	dbSNP
rs1479776559	27	dbSNP
rs762202381	28	dbSNP
rs370043877	29	dbSNP
rs1171480059	30	dbSNP
rs1342654086	32	dbSNP
rs764648651	34	dbSNP
rs1438099564	49	dbSNP
rs759150645	50	dbSNP
rs1428047645	54	dbSNP
rs1224824208	62	dbSNP
rs1366236917	67	dbSNP
rs979781433	72	dbSNP
rs1406897654	73	dbSNP
rs577772489	74	dbSNP
rs8179163	74	dbSNP
rs1366772576	82	dbSNP
rs1215093760	85	dbSNP
rs1282075105	96	dbSNP
rs1297383245	100	dbSNP
rs878903165	105	dbSNP
rs1382483567	110	dbSNP
rs971569417	113	dbSNP
rs1292105087	116	dbSNP
rs1024036334	123	dbSNP
rs1287522048	128	dbSNP
rs1488405404	141	dbSNP
rs938266874	152	dbSNP
rs539718608	163	dbSNP
rs1448087003	164	dbSNP
rs902604879	170	dbSNP
rs1046557932	171	dbSNP
rs1407493935	173	dbSNP
rs1478111758	174	dbSNP
rs897961688	175	dbSNP
rs779888123	176	dbSNP
rs1006517702	187	dbSNP
rs1804291	195	dbSNP
rs1430550117	196	dbSNP
rs531790638	202	dbSNP
rs1434162861	205	dbSNP
rs564336340	208	dbSNP
rs1804290	211	dbSNP
rs1361065128	217	dbSNP
rs35707140	218	dbSNP
rs771941115	220	dbSNP
rs1226266972	223	dbSNP
rs1288507924	223	dbSNP
rs1317055685	223	dbSNP
rs1217090162	225	dbSNP
rs185246219	227	dbSNP
rs1463952597	230	dbSNP
rs1188281510	232	dbSNP
rs931569549	234	dbSNP
rs920155746	238	dbSNP
rs1161740592	243	dbSNP
rs1413163225	247	dbSNP
rs1401402995	248	dbSNP
rs550603153	251	dbSNP
rs1485383372	255	dbSNP
rs15932	257	dbSNP
rs1237721460	258	dbSNP
rs1384791964	261	dbSNP
rs1445347753	264	dbSNP
rs1283273945	270	dbSNP
rs1373400719	282	dbSNP
rs961562038	283	dbSNP
rs1265730123	284	dbSNP
rs1327618641	290	dbSNP
rs1205547947	291	dbSNP
rs111455603	292	dbSNP
rs1264129760	299	dbSNP
rs1321308055	302	dbSNP
rs528847135	310	dbSNP
rs1262010317	311	dbSNP
rs11552764	319	dbSNP
rs14566	328	dbSNP
rs1185655298	333	dbSNP
rs546123000	343	dbSNP
rs1470650090	345	dbSNP
rs1184392000	347	dbSNP
rs1409862001	350	dbSNP
rs546681101	351	dbSNP
rs901926608	352	dbSNP
rs1040400468	354	dbSNP
rs946125121	355	dbSNP
rs8179164	358	dbSNP
rs1054198606	359	dbSNP
rs564679250	360	dbSNP
rs1032222144	361	dbSNP
rs1285844110	374	dbSNP
rs927205465	375	dbSNP
rs1228314785	376	dbSNP
rs1284532224	380	dbSNP
rs999442712	381	dbSNP
rs1451143171	386	dbSNP
rs1199047108	392	dbSNP
rs902381853	395	dbSNP
rs1046250443	398	dbSNP
rs980196711	399	dbSNP
rs546223846	409	dbSNP
rs971050652	410	dbSNP
rs1249702762	414	dbSNP
rs1472326790	428	dbSNP
rs895208916	432	dbSNP
rs1055542094	436	dbSNP
rs931290946	438	dbSNP
rs531246506	439	dbSNP
rs1463537730	443	dbSNP
rs1371560344	453	dbSNP
rs972511608	469	dbSNP
rs1431240945	472	dbSNP
rs1305872648	475	dbSNP
rs867177034	477	dbSNP
rs940263468	481	dbSNP
rs560386296	482	dbSNP
rs1405082753	490	dbSNP
rs912751195	502	dbSNP
rs961114947	502	dbSNP
rs1231721236	507	dbSNP
rs1274611500	508	dbSNP
rs986904087	512	dbSNP
rs1015129678	517	dbSNP
rs1230843166	518	dbSNP
rs1322307777	519	dbSNP
rs563595148	526	dbSNP
rs773654948	535	dbSNP
rs1006363504	537	dbSNP
rs1804289	541	dbSNP
rs772450048	544	dbSNP
rs781072946	550	dbSNP
rs1223263648	554	dbSNP
rs1459951310	566	dbSNP
rs954477111	566	dbSNP
rs755054356	572	dbSNP
rs1198148203	574	dbSNP
rs974817775	580	dbSNP
rs1029207252	583	dbSNP
rs996585911	584	dbSNP
rs748474798	592	dbSNP
rs901875256	597	dbSNP
rs957769165	598	dbSNP
rs1389120530	599	dbSNP
rs8857	603	dbSNP
rs192200777	604	dbSNP
rs779217505	606	dbSNP
rs751739884	608	dbSNP
rs1164424004	610	dbSNP
rs1040349436	611	dbSNP
rs1387973113	612	dbSNP
rs1385587095	620	dbSNP
rs1010298035	621	dbSNP
rs1032417305	622	dbSNP
rs891870303	624	dbSNP
rs1366348851	633	dbSNP
rs1437660333	635	dbSNP
rs978033483	638	dbSNP
rs1341638073	639	dbSNP
rs1054231783	641	dbSNP
rs1267289633	643	dbSNP
rs1354416765	645	dbSNP
rs1209134533	652	dbSNP
rs1024833294	656	dbSNP
rs966599590	656	dbSNP
rs1481291546	659	dbSNP
rs935828667	676	dbSNP
rs1189265359	677	dbSNP
rs574460264	677	dbSNP
rs1457010981	678	dbSNP
rs1158662218	680	dbSNP
rs1013390476	681	dbSNP
rs1459286931	684	dbSNP
rs1226317525	687	dbSNP
rs1359665045	688	dbSNP
rs1317644287	692	dbSNP
rs1244172728	703	dbSNP
rs574229490	710	dbSNP
rs553086216	727	dbSNP
rs76540530	728	dbSNP
rs1315156429	734	dbSNP
rs1338755783	750	dbSNP
rs1393640703	753	dbSNP
rs878939570	755	dbSNP
rs1342367431	756	dbSNP
rs1394495868	757	dbSNP
rs1331050076	759	dbSNP
rs868283171	761	dbSNP
rs1297658912	764	dbSNP
rs1461632653	765	dbSNP
rs1044285905	767	dbSNP
rs950000721	773	dbSNP
rs1422910175	777	dbSNP
rs1181814538	780	dbSNP
rs1413547608	786	dbSNP
rs573002067	793	dbSNP
rs1367642101	797	dbSNP
rs8179165	798	dbSNP
rs550778932	800	dbSNP
rs1466919986	802	dbSNP
rs766638054	807	dbSNP
rs898523472	808	dbSNP
rs1367826548	812	dbSNP
rs9609	812	dbSNP
rs1182526155	817	dbSNP
rs1289008997	817	dbSNP
rs1352455648	817	dbSNP
rs909608517	832	dbSNP
rs985366511	833	dbSNP
rs1235059546	834	dbSNP
rs1254061807	841	dbSNP
rs1036974025	842	dbSNP
rs1247849504	843	dbSNP
rs1194694841	851	dbSNP
rs539216533	853	dbSNP
rs1205490941	857	dbSNP
rs1262148005	860	dbSNP
rs940293155	864	dbSNP
rs952211936	865	dbSNP
rs891347230	876	dbSNP
rs187696803	879	dbSNP
rs1359255674	880	dbSNP
rs1051215537	881	dbSNP
rs932784832	889	dbSNP
rs1261664944	890	dbSNP
rs3448	891	dbSNP
rs966675633	893	dbSNP
rs1465709533	897	dbSNP
rs1334476208	903	dbSNP
rs1306429132	905	dbSNP
rs1408017539	909	dbSNP
rs974554726	912	dbSNP
rs936405303	914	dbSNP
rs1405580768	921	dbSNP
rs535456222	930	dbSNP
rs8179166	934	dbSNP
rs3191115	947	dbSNP
rs546405545	950	dbSNP
rs891901421	952	dbSNP
rs528185207	955	dbSNP
rs570412996	960	dbSNP
rs3204561	962	dbSNP
rs1489063535	964	dbSNP
rs1158913040	983	dbSNP
rs966630409	986	dbSNP
rs1265665544	987	dbSNP
rs1024858032	988	dbSNP
rs11552766	990	dbSNP
rs1428100340	997	dbSNP
rs1167988031	998	dbSNP
rs552742843	1001	dbSNP
rs72554687	1002	dbSNP
rs11552765	1009	dbSNP
rs765764554	1018	dbSNP
rs950022212	1020	dbSNP
rs879614279	1026	dbSNP
rs895723248	1029	dbSNP
rs1435470363	1041	dbSNP
rs1036716453	1042	dbSNP
rs762171413	1044	dbSNP
rs1299548744	1052	dbSNP
rs78558201	1054	dbSNP
rs1362528784	1062	dbSNP
rs563654524	1065	dbSNP

- Nature medicine, 2007

Growing evidence indicates that microRNAs (miRNAs or miRs) are involved in basic cell functions and oncogenesis. Here we report that miR-133 has a critical role in determining cardiomyocyte hypertrophy. We observed decreased expression of both miR-133 and miR-1, which belong to the same transcriptional unit, in mouse and human models of cardiac hypertrophy. In vitro overexpression of miR-133 or miR-1 inhibited cardiac hypertrophy. In contrast, suppression of miR-133 by 'decoy' sequences induced hypertrophy, which was more pronounced than that after stimulation with conventional inducers of hypertrophy. In vivo inhibition of miR-133 by a single infusion of an antagomir caused marked and sustained cardiac hypertrophy. We identified specific targets of miR-133: RhoA, a GDP-GTP exchange protein regulating cardiac hypertrophy; Cdc42, a signal transduction kinase implicated in hypertrophy; and Nelf-A/WHSC2, a nuclear factor involved in cardiogenesis. Our data show that miR-133, and possibly miR-1, are key regulators of cardiac hypertrophy, suggesting their therapeutic application in heart disease.

- American journal of respiratory and critical care medicine, 2009

RATIONALE: Augmented bronchial smooth muscle (BSM) contraction is one of the causes of bronchial hyperresponsiveness. The protein RhoA and its downstream pathways have now been proposed as a new target for asthma therapy. MicroRNAs (miRNAs) play important roles in normal and diseased cell functions, and a contribution of miR-133 to RhoA expression has been suggested in cardiomyocytes. OBJECTIVES: To make clear the mechanism(s) of up-regulation of RhoA observed in the BSMs of experimental asthma, the role of miR-133a in RhoA expression was tested. METHODS: Total proteins and RNAs (containing miRNAs) were extracted from cultured human BSM cells (hBSMCs) that were treated with antagomirs and/or IL-13, and bronchial tissues of BALB/c mice that were sensitized and repeatedly challenged with ovalbumin. RhoA protein and miR-133a were detected by immunoblotting and quantified real-time reverse transcriptase-polymerase chain reaction, respectively. MEASUREMENTS AND MAIN RESULTS: In hBSMCs, an up-regulation of RhoA was observed when the function of endogenous miR-133a was inhibited by its antagomir. Treatment of hBSMCs with IL-13 caused an up-regulation of RhoA and a down-regulation of miR-133a. In bronchial tissues of the repeatedly ovalbumin-challenged mice, a significant increase in RhoA was observed. Interestingly, the level of miR-133a was significantly decreased in BSMs of the challenged mice. CONCLUSIONS: These findings suggest that RhoA expression is negatively regulated by miR-133a in BSMs. IL-13 might, at least in part, contribute to the reduction of miR-133a.