miRTarBase - #MIRT001170


—
pre-miRNA Information
pre-miRNA mmu-mir-32   
Genomic Coordinates chr4: 56895229 - 56895298
Synonyms Mirn32, mmu-mir-32, Mir32
Description Mus musculus miR-32 stem-loop
Comment Mouse mir-32 is predicted .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-32-5p
Sequence 6| UAUUGCACAUUACUAAGUUGCA |27
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Enpp6   
Synonyms 4833421B01Rik, B830047L21Rik, D8Ertd514e, E-NPP 6, GPC-Cpde, Npp6
Description ectonucleotide pyrophosphatase/phosphodiesterase 6
Transcript NM_177304   
Expression
Putative miRNA Targets on Enpp6
3'UTR of Enpp6
(miRNA target sites are highlighted)		 
>Enpp6|NM_177304|3'UTR
   1 CTGGCCCTATGGCTCATTCCAAAGCACTGTTGCAGTAAAGCCTGCTTCCAACATGGGACAGTTTTCATTTTCTTTATGGA
  81 ATAATAGCTTTATTAACACAATCAAGGCCGTTAAAGTTGTGAATATATTATTCTTGGGTGATTCTACCCACAAAAGTCCC
 161 TTCTGGGGAAAAAAACTGCAAAATTCGTATACTTTGTTTTACCTAAAAAGTTTGAAATTTGCATCTTCTCATTCACTTTT
 241 CTACATAGTTCTCTGCTTTGTTTATACATCCTTACTGAAGATGAACAGTGAGAGCCATGCTTTGCCCCTGCAACAGGCAA
 321 ACATTAAACGGGTATCCTGTAGTCATCTTGGACCCTCTCATACTGCCTTGCCCTGTCAAGCAGGCTCTCTGTGATCTGTC
 401 CACATGTAAGAGCTCACACTTGGAGGTCTGCAGCCATAATGTTATATTTTCTATTCTATTCCAGATAGAGACAGTCTATC
 481 TACAGGAAAGCAATATGTGTTGTCTGGTTCTTCCCCAATGCATGGTGGTTTTTTTTTGTTTTTTGTTTTTTGTTTTTTTA
 561 ACAACACAATCTCAGAAAGCACATAGACCAGTAGAAATCACAACCAATTGAAGTATCAATAACAATTAGACAACTCATAG
 641 AACTGGCAGAGGCCATGACACCTCTCAAAATTTGAGAACAGAATAGCTTCTCCCCTTCATTGAATGCCAAGTTTCAAGGT
 721 TCTATGTAACTAAAAACACAATCTCTCATAATACAAAGCCTTGTGAGCCACAGGCCCTGAACATTGACATCCACATCTGC
 801 CTGTCGGTGACCACTAACTTCCCTCAGACACTGCTACTCTTTTAGTTTTTGAGTCAAGGCACATGTCTGATATGTCAGTG
 881 AAGGCTTGGAAAGGAAGCCTTAGTATCATAGTATGAACATCATAAATTTAACTCTTTGCTAGGAATAGTGATATATCAAA
 961 TGCAAGTTCCATACTTCAGTAGTTCAGAAGGACCTTGTAAAACAAGGACTGATTGTACTTTTAAACAATTAGAAGAGAGC
1041 CTACATGTACACACACATGCACACACATTTACACATAAACACATATACACATGCACACACACATGTATGCACATGCACAG
1121 ACATAAACATATACATGAATATAAACACACACACACGTGTGTGAACACATGCCCTCCACATATTATGGTGATACCTAGAA
1201 TTTTTATCTTTTTTATGATGAAGAAGCATCATAAAGCATCAAATTCAAAAGAATTTGAGTTTTGAATTTGTCCATTTTCT
1281 CAGCTACTGTTATGCCATGTGACACTGTCTCACCAGGCTGGCCAGAAGCAGCAACAAACCGGAGCTCCCAACCAGCCACA
1361 TGATCCTGGCTGTGGGCCTCAATATCCTAGAGCACACGGTGCCGCTAAACTATGAGGGTCTGTAGGGTGGTGTATGAAAG
1441 TCCTGTTGACTAAATAATAGTTTCAGTTCATGATAGGCTTATGGGCATGTGACCTCATCCTATGTTGAGGAGATTATGAA
1521 TCCTGTTGCCTTTGAAATGAGTTGGCACTAAATGGGTCATTAAAAGTGACACTGTGTCAGGAAAAGACAAAACATCAGCT
1601 CTCAGGAGGAGGAAGCCTTTATTTGGTGCTTGCCCTGAAAAAAAAACAAAAAACAAAACAAAACAAAACAAAAAACCCCA
1681 AAACCGAAAACAAAACAAAACAAAACAAAAAAAACTAACAAAACAAAACAACAAACAAACAAAAAGCTCAATAGCACCTG
1761 AGTTTCGCAAAGATGGAGTTGCTGCAGTGTCTTGAACTAAGAGAGCAAAAGATCACTGCTTAAAAAAATGTTGAGTTTAT
1841 TCTCACAAAAGATTTCTAAGGATTTAGGTAATGTGTCTTTAAAGCTCCAACAAACCATGGAATCAACTGGGAAGGAGGCG
1921 AATTAGCCTTTGTTGTGTGGTTGAAAGCAAATTAGTGCTTTCAGAACACTGGTAGGGGCTGTTGAAACAGACATCTATTG
2001 TTCAAAGTTTTCTTTTCCCTTCAAGAATGGTATTTTGTTGAATTCCAGGAAGCCAGTTTGACTTCCTCTTTAGAAGAAGG
2081 ATTTCTGAGACACATTCAAGTGATTAAATTCAAAGCAAATTGTCAACATCACAAGGGGGATGCAATGTTGCATCTTTCTT
2161 TCTGGTTCTTTCCTTCACTGGTCTTTAGGTTATATAACAAAATCTCAGATATCTACCTTACAAAGTATAATGTTCACTAA
2241 ATGAATGAGGAAAAAGATACCAAAGGAAAAAAGTGTAGAGGCACATAATAATAATACAATTTAGGGTTAAAAAAAGTCTT
2321 TTGCCTAAAAATAATGCATAGATTCTAATGACATAATTAGTTGGCTGAAAGGATCTTCCTATTAATTTTGCTTTAAAGTC
2401 TAATTTTTTTCCCCAGTTAATATCTCCCACCTACTATTAACCCTCCCTGCATAGCCTCTGCAATTCACTGACAGTATATT
2481 CCTGGTGCCAGGGCTGTAGACTTCACTTTCTCTAAACGTGATGTGCTCAGTAGAAGTAAGATGTGCTAGGAATATGCACG
2561 AACATGCTCCCTCTTTAAGTTTCCTGAGAATTTCCTGCTAAGCCCAGGAGGAAGATAACTGGGTGGACCTACCACAGAGA
2641 AATGAAAGGGATGGTCTTTAAATTAAAGATGAGACTAAGGGCTACTTTCTCTACCCATGTGCCTCATCTCCAGGATCCAA
2721 TTTAATTAAGTTCTGAAGACCTAACCATAAACTATAAAAGGTCCAGGGAATTCTTGCAAGCCTAGAAGAGTCTTAAATTA
2801 TAAAGGCCCAATTCTCTTCACTTTAAAAGAAACTTTGGAAATCCATATCAGATCTGAGTTCAACCACAGACAGTACCTTG
2881 CAATCTCTCCCAGAGTTGCAGAGACAGGGTCTCAGGCAACCCTTGTAAGTCATAATAATGAAGGTTTGGGCTTGCTCCAA
2961 GGAGAAGTGGGGTTTGCTTCAATCCTGGATTATCTGAATCCTCAGAGAGCTATTTCATAATTAGCCAGTTCTTCTGGGGC
3041 TCATCAGCTGACACTGGCCACTGACCAACCCCATATGGATCAAGCTTCCATCCCTCCAACACCAAAGAGGAGGTCTCAAA
3121 GCTCTTAAGGGGAACGAAACTTGTAGAGAAAAAAAAAAGGCAGGTGGCTTGCCTTTCACATGTCCCTCCTTGTGGCTCTA
3201 GGAGTCCCCATGGTGCTATCAAACCAACTTGGACATTATTCAAGAAGCACAACTCTTCAGTGGAATTTTCTAAGTGTTTC
3281 ACATTTGTATGAATGTCAAGAGATTTCCAAGTGTGATTTGCATTTTTACCATTATAAGCAACCTGTGATGGATCAAAGTC
3361 AGATGCTATAAATTATTTGCTACAGAAAATGAGAACATAGCCAATGCCCACTGGCTGTTGAACATTTCAAAACTTGGTTT
3441 TTAGCTTATTCTCAGGAGAATACGTGTTGCTTAGAAATCATAAAATGTCACCTAGTACAATGTATTAAATGTTTATATTT
3521 TGAATGAAAAAAAAAAGAATAAATGTCAACAGGGAGACTTAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' acGU-UGAAUCAUUACACGUUAu 5'
            || ||  ||||   |||||| 
Target 5' caCAGAC--AGTACCTTGCAATc 3'
 2865 - 2885 124.00 -8.00
2 
miRNA  3' acGUUGAA----UCAUUACACGUUAu 5'
            |||| |    || ::  |||||| 
Target 5' gtCAACATCACAAGGGGGATGCAATg 3'
 2122 - 2147 122.00 -9.00
3 
miRNA  3' acguugaaucauuacACGUUAu 5'
                         |||||| 
Target 5' tccctgcatagcctcTGCAATt 3'
 2444 - 2465 120.00 -5.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions COS-7
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... "Both 3 UTR sequences resulted in reduced luciferase activity in the presence of miR-219 compared with negative vector controls. To further assess whether the suppression of both genes is miR-219 specific ...

- Shin D; Shin JY; McManus MT; Ptacek LJ; Fu YH, 2009, Annals of neurology.

Article - Shin D; Shin JY; McManus MT; Ptacek LJ; Fu YH
- Annals of neurology, 2009
OBJECTIVE: MicroRNAs (miRNAs) regulate gene expression and have many roles in the brain, but a role in oligodendrocyte (OL) function has not been demonstrated. METHODS: A Dicer floxed conditional allele was crossed with the proteolipid protein promoter-driven inducible Cre allele to generate inducible, OL-specific Dicer-floxed mice. RESULTS: OL-specific Dicer mutants show demyelination, oxidative damage, inflammatory astrocytosis and microgliosis in the brain, and eventually neuronal degeneration and shorter lifespan. miR-219 and its target ELOVL7 (elongation of very long chain fatty acids protein 7) were identified as the main molecular components that are involved in the development of the phenotype in these mice. Overexpressing ELOVL7 results in lipid accumulation, which is suppressed by miR-219 co-overexpression. In Dicer mutant brain, excess lipids accumulate in myelin-rich brain regions, and the peroxisomal beta-oxidation activity is dramatically reduced. INTERPRETATION: Postnatal Dicer ablation in mature OLs results in inflammatory neuronal degeneration through increased demyelination, lipid accumulation, and peroxisomal and oxidative damage, and therefore indicates that miRNAs play an essential role in the maintenance of lipids and redox homeostasis in mature OLs that are necessary for supporting axonal integrity as well as the formation of compact myelin.
LinkOut: [PMID: 20035504]
17 mmu-miR-32-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001170 Enpp6 ectonucleotide pyrophosphatase/phosphodiesterase 6 3 1
MIRT578184 Ncam2 neural cell adhesion molecule 2 1 1
MIRT580675 Stk10 serine/threonine kinase 10 1 3
MIRT583673 Fam136a family with sequence similarity 136, member A 1 1
MIRT584194 Cpeb1 cytoplasmic polyadenylation element binding protein 1 1 1
MIRT587065 Gm5148 predicted gene 5148 1 2
MIRT589037 Rhbdl3 rhomboid, veinlet-like 3 (Drosophila) 1 1
MIRT595173 Zpbp zona pellucida binding protein 1 1
MIRT595804 Chm choroidermia (RAB escort protein 1) 1 1
MIRT595905 Cd69 CD69 antigen 1 1
MIRT596290 Tagap1 T cell activation GTPase activating protein 1 1 1
MIRT596305 Tagap T cell activation Rho GTPase activating protein 1 1
MIRT596474 Btg2 B cell translocation gene 2, anti-proliferative 1 1
MIRT598601 Il5ra interleukin 5 receptor, alpha 1 1
MIRT598838 Gm14420 zinc finger protein 970 1 1
MIRT601411 Uba1y ubiquitin-activating enzyme, Chr Y 1 1
MIRT606064 Zfp300 zinc finger protein 300 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-32 Medpor NULL NULL Microarray osteoblast-like cells line (MG-63) 18408260 2008 down-regulated
miR-32 Dihydrotestosterone(DHT) NULL 10635 Microarray prostate cancer 20945501 2011 up-regulated
miR-32 Ginsenoside Rh2 NULL 119307 Microarray human glioma cells U251 21372826 2011 down-regulated
miR-32 1alpha,25-Dihydroxyvitamin D3 approved 5280453 Quantitative real-time PCR acute myeloid leukemia 21816906 2011 up-regulated
miR-32 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-32 Ethanol NULL 702 Quantitative real-time PCR CIE10 22141737 2012 up-regulated
miR-32 5a-dihydrotestosterone (DHT) NULL 15 Quantitative real-time PCR LNCaP cells 22266859 2012 up-regulated
miR-32 Progesterone approved 5994 Microarray Breast cancer 22330642 2012 down-regulated
miR-32 Bicalutamide approved 2375 Microarray prostate 22674191 2012 down-regulated
miR-32 Bicalutamide approved 2375 Quantitative real-time PCR LNCaP cells 22674191 2012 down-regulated
miR-32 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated
miR-32 Galactose NULL 6036 Quantitative real-time PCR lens 22736950 2012 down-regulated
miR-32 N-ethyl-N-nitrosourea NULL 12967 Quantitative real-time PCR mouse liver 21029445 2010 up-regulated
miR-32 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated

Error report submission