miRTarBase - #MIRT001716


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pre-miRNA Information
pre-miRNA mmu-mir-125a   
Genomic Coordinates chr17: 17830812 - 17830879
Description Mus musculus miR-125a stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-125a-5p
Sequence 6| UCCCUGAGACCCUUUAACCUGUGA |29
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Lin28a   
Synonyms AL024421, Gm10299, Lin-28, Lin28, Tex17, lin-28A
Description lin-28 homolog A (C. elegans)
Transcript NM_145833   
Expression
Putative miRNA Targets on Lin28a
3'UTR of Lin28a
(miRNA target sites are highlighted)		 
>Lin28a|NM_145833|3'UTR
   1 GGCCCAGGAGTCAGGGTTATTCTTTGGCTAATGGGGAGTTTAAGGAAAGAGGCATCAATCTGCAGAGTGGAGAAAGTGGG
  81 GGTAAGGGTGGGTTGCGTGGGTAGCTTGCACTGCCGTGTCTCAGGCCGGGGTTCCCAGTGTCACCCTGTCTTTCCTTGGA
 161 GGGAAGGAAAGGATGAGACAAAGGAACTCCTACCACACTCTATCTGAAAGCAAGTGAAGGCTTTTGTGGGGAGGAACCAC
 241 CCTAGAACCCGAGGCTTTGCCAAGTGGCTGGGCTAGGGAAGTTCTTTTGTAGAAGGCTGTGTGATATTTCCCTTGCCAGA
 321 CGGGAAGCGAAACAAGTGTCAAACCAAGATTACTGAACCTACCCCTCCAGCTACTATGTTCTGGGGAAGGGACTCCCAGG
 401 AGCAGGGCGAGGTTATTTTCACACCGTGCTTATTCATAACCCTGTCCTTTGGTGCTGTGCTGGGAATGGTCTCTAGCAAC
 481 GGGTTGTGATGACAGGCAAAGAGGGTGGTTGGGGAGACAACTGCTGACCTGCTGCCCACACCTCACTCCCAGCCCTTTCT
 561 GGGCCAATGGGATTTTAATTTATTTGCTCCCTTAGGTAACTGCACCTTGGGTCCCACTTTCTCCAGGATGCCAACTGCAC
 641 TATCTACGTGCGAATGACGTATCTTGTGCGTTTTTTTTTTTTTTAATTTTTAAAATTTTTTTTCATCTTCTTAATATAAA
 721 TAATGGGTTTGTATTTTTGTATATTTTAATCTTAAGGCCCTCATTCCTGCACTGTGTTCTCAGGTACATGAGCAATCTCA
 801 GGGATAATAAGTCCGTAGCAGCTCCAGGTCTGCTCAGCAGGAATACTTTGTTTTGTTTTGTTTTGATCACCATGGAGACC
 881 AACCATTTGGAGTGCACAGCCTGTTGAACTACCTCATTTTTGCCGATTACAGCTGGCTTTTCTGCCATAGCGTCCTTGAA
 961 AAATGTGTCTCACGGGTTTCGATTGAGCTGCCCCAAGACTTGATCTGGATTTGGCAAAACATAGGACATCACTCTAAACA
1041 GGAAAGGGTGGTACAGAGACATTAAAAGGCTGGGCCAGGTGAAAGGCACAAGAGGAACTTTCCATACCAGATCCATCCTT
1121 TTGCCAGATTAGTGGAAGCCTGCCATGCACAGCAGGGTGTGAGAGAGAGAGTGTGTATGTATGTGTGTGTGGATTTTTTT
1201 TAATGCAAATTTATGAAGACGAGGTGGGTTTTGTTTATTTGATTGCTTTTTGTGCTGGGGATGGAATCTTGGGCTTCATT
1281 TGTGCTAGGAAGTACACTGCCACTGAGTTATCCCAGTAAGAATGCAACTTAAGACCAGTACCCTTATTCCCACACTGTGC
1361 TGTCCAGGCATGGGAACATGAGGCAGGGACTCAACTCCTTAGCCTTTCACAATCTTGGCTTTCTGAGAGACTCATGAGTA
1441 TGGGCCTCAGTGGCAAGTGTCCTGCCCTGCTGTAGCGTGATGGTTGATAGCTAAAGGAAAGAGGGGGTGGGGAGTTTCGT
1521 TTACATGCTTTGAGATCGCCACAAACCTACCTCACTGTGTTGAAACGGGACAAATGCAATAGAACACATTGGGTGGTGTG
1601 TGTGTGTGTCTGATCTTGGTTTCTTGTCTCCCTCTCCCCCCAAATGCTGCCCTCACCCCTAGTTAATTGTATTCGTCTGG
1681 CCTTTGTAGGACTTTTACTGTCTCTGAGTTGGTGATTGCTAGGTGGCCTAGTTGTGTAAATATAAATGTGTTGGTCTTCA
1761 TGTTCTTTTGGGGTTTTATTGTTTACAAAACTTTTGTTGTATTGAGAGAAAAATAGCCAAAGCATCTTTGACAGAAAGCT
1841 CTGCACCAGACAACACCATCTGAAACTTAAATGTGCGGTCCTCTTCTCAAAGTGAACCTCTGGGACCATGGCTTATCCTT
1921 ACCTGTTCCTCCTGTGTCTCCCATTCTGGACCACAGTGACCTTCAGACAGCCCCTCTTCTCCCTCGTAAGAAAACTTAGG
2001 CTCATTTACTTCTTTGAGCATCTCTGTAACTCTTGAAGGACCCATGTGAAAATTCTGAAGAAGCCAGGAACCTCATTCTT
2081 TCCTTGTCCCTAACTCAGTGAAGAGTTTTGGTTGGTGGTTTTGAGACAGGGCCTCACTCTGTAGCTGGAGATAGAGAGCC
2161 TCGGGTTCCTGGCTCTCCTCCTGCCTTCTGCACAGAGTCCCCTGTGCAGGGATTGCAGGTGCCGCTTCTCCCTGGCAAGA
2241 CCATTTATTTCATGGTGTGATTCGCCTTTGGATGGATCAAACCAATGTAATCTGTCACCCTTAGGTCGAGAGAAGCAATT
2321 GTGGGGCCTTCCATGTAGAAAGTTGGAATCTGGACACCAGAAAAGGGACTATGAATGTACAGTGAGTCACTCAGGAACTT
2401 AATGCCGGTGCAAGAAACTTATGTCAAAGAGGCCACAAGATTGTTACTAGGAGACGGACGAATGTATCTCCATGTTTACT
2481 GCTAGAAACCAAAGCTTTGTGAGAAATCTTGAATTTATGGGGAGGGTGGGAAAGGGTGTACTTGTCTGTCCTTTCCCCAT
2561 CTCTTTCCTGAACTGCAGGAGACTAAGGCCCCCCACCCCCCGGGGCTTGGATGACCCCCACCCCTGCCTGGGGTGTTTTA
2641 TTTCCTAGTTGATTTTTACTGTACCCGGGCCCTTGTATTCCTATCGTATAATCATCCTGTGACACATGCTGACTTTTCCT
2721 TCCCTTCTCTTCCCTGGGAAAATAAAGACTTATTGGTACTCCAGAGTTGGTACTG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aguGUCCA---AUUUCCCAGAGUCCCu 5'
             |||||   |:|:   |||||||| 
Target 5' tctCAGGTACATGAGCAATCTCAGGGa 3'
 778 - 804 161.00 -22.90
2 
miRNA  3' agUGUCCAAUUU-----CCCAGAGUCCcu 5'
            ::|| ||: |     | ||||||||  
Target 5' ggGTAGCTTGCACTGCCGTGTCTCAGGcc 3'
 99 - 127 138.00 -17.90
3 
miRNA  3' agUGUCCAAUUUCCCAGAGUCCcu 5'
            ||||  | :||   ||||||  
Target 5' gtACAG--T-GAGTCACTCAGGaa 3'
 2377 - 2397 129.00 -13.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Article - Wu L; Belasco JG
- Molecular and cellular biology, 2005
Vertebrate genomes each encode hundreds of micro-RNAs (miRNAs), yet for few of these miRNAs is there empirical evidence as to which mRNA(s) they regulate. Here we report the identification of human lin-28 mRNA as a regulatory target of human miR-125b and its homolog miR-125a. Studies of miR-125b function in mouse P19 embryonal carcinoma cells induced to develop into neurons suggest a role for this regulatory miRNA in mammalian neuronal differentiation, since its increased concentration in these cells contributes to lin-28 downregulation. Within the lin-28 3' untranslated region (UTR) are two conserved miRNA responsive elements (miREs) that mediate repression by miR-125b and miR-125a. Simultaneous deletion of both miREs renders the lin-28 3' UTR almost completely insensitive to these miRNAs, indicating that these two miREs are the principal elements in the lin-28 3' UTR that respond to miR-125. At the 3' end of each element is an adenosine residue that makes a significant contribution to function irrespective of its complementarity to the 5'-terminal nucleotide of miR-125. By contrast to most earlier reports of gene repression by other miRNAs that are imperfectly complementary to their targets, lin-28 downregulation by miR-125 involves reductions in both translational efficiency and mRNA abundance. The decrease in the mRNA concentration is achieved by a posttranscriptional mechanism that is independent of the inhibitory effect on translation.
LinkOut: [PMID: 16227573]
28 mmu-miR-125a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001716 Lin28a lin-28 homolog A (C. elegans) 1 1
MIRT005597 Trim71 tripartite motif-containing 71 4 3
MIRT006310 Cbx7 chromobox 7 2 1
MIRT006967 4632428N05Rik V-set immunoregulatory receptor 2 1
MIRT054177 Ptpn18 protein tyrosine phosphatase, non-receptor type 18 2 1
MIRT054178 Ptpn7 protein tyrosine phosphatase, non-receptor type 7 2 1
MIRT054179 Ppp1ca protein phosphatase 1, catalytic subunit, alpha isoform 2 1
MIRT054180 Ppp2ca protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform 2 1
MIRT054570 Traf6 TNF receptor-associated factor 6 3 1
MIRT580227 Tspan12 tetraspanin 12 2 2
MIRT582368 Mtf1 metal response element binding transcription factor 1 2 2
MIRT582450 Mgat4a mannoside acetylglucosaminyltransferase 4, isoenzyme A 2 2
MIRT584884 Antxr2 anthrax toxin receptor 2 2 2
MIRT587193 Foxk1 forkhead box K1 2 2
MIRT588848 Snrnp40 small nuclear ribonucleoprotein 40 (U5) 2 2
MIRT591275 Lars2 leucyl-tRNA synthetase, mitochondrial 2 4
MIRT592914 Madd MAP-kinase activating death domain 2 2
MIRT594893 Il1rn interleukin 1 receptor antagonist 2 4
MIRT596427 Hif1an hypoxia-inducible factor 1, alpha subunit inhibitor 3 3
MIRT599142 Dqx1 DEAQ RNA-dependent ATPase 2 2
MIRT599717 Aph1c aph1 homolog C, gamma secretase subunit 2 2
MIRT600832 Gm14137 predicted gene 14137 2 2
MIRT604902 Jmy junction-mediating and regulatory protein 2 2
MIRT605441 Stat1 signal transducer and activator of transcription 1 2 2
MIRT606092 Wdr25 WD repeat domain 25 2 2
MIRT732537 Tnfrsf1b tumor necrosis factor receptor superfamily, member 1b 4 0
MIRT734700 VDR vitamin D receptor 2 0
MIRT734824 Sox11 SRY (sex determining region Y)-box 11 2 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-125a Budesonide approved 5281004 Microarray neonatal mice liver 20145010 2010 down-regulated
miR-125a Phenethyl isothiocyanate(PEITC) NULL 16741 Microarray neonatal mice lung 20145010 2010 down-regulated
miR-125a Chaihu Shugan San NULL NULL Microarray hippocampus 23947143 2013 down-regualted
miR-125a Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated
miR-125a Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 up-regulated
miR-125a Enoxacin approved 3229 Quantitative real-time PCR HEK293 cells 18641635 2008 up-regulated
miR-125a 4-hydroxynonenal NULL 5283344 Microarray human leukemic HL-60 cell 19022373 2009 up-regulated
miR-125a Valproate approved 3121 Quantitative real-time PCR CD4+, CD25- T cells 20427269 2010 down-regulated
miR-125a Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-125a Enoxacin approved 3229 Northern blot HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-125a Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-125a 5-aza-2'-deoxycytidine (5-Aza-CdR) approved 451668 Microarray breast cancer MDA-MB231 22076154 2011 down-regulated
miR-125a-5p Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 up-regulated
miR-125a-5p Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 down-regulated
miR-125a-5p Curcumin NULL 969516 Microarray HONE1 cells 24896104 2014 down-regulated
miR-125a-5p 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-125a-5p Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-125a-5p Trastuzumab approved NULL Microarray SKBR3 cells. 22384020 2012 up-regulated
miR-125a-5p Glucocorticoid NULL NULL TaqMan low-density array Eosinophilic esophagitis 22815788 2012 up-regulated
miR-125a-5p Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-125a-5p Paclitaxel 36314 NSC125973 approved sensitive Low Colon Cancer tissue and cell line (HT-29)

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