miRTarBase - #MIRT003124


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pre-miRNA Information
pre-miRNA mmu-mir-122   
Genomic Coordinates chr18: 65248861 - 65248926
Synonyms Mirn122a, mmu-mir-122, Mir122a
Description Mus musculus miR-122 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-122-5p
Sequence 6| UGGAGUGUGACAAUGGUGUUUG |27
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Plasma Microarrays; Real-time quantitative RT-PCR analysis
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Decrease Liver tissue Microarrays; Real-time quantitative RT-PCR analysis
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Decrease Liver tissue Quantitative real-time reverse transcription PCR
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Plasma Quantitative real-time reverse transcription PCR
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Plasma Quantitative reverse transcription PCR
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data expression Increase Serum Quantitative reverse transcription PCR
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression . Blood Polymerase chain reaction
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Serum Quantitative polymerase chain reaction
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Higher Blood Polymerase chain reaction
Gene Information
Gene Symbol Ccng1   
Synonyms AI314029
Description cyclin G1
Transcript NM_009831   
Expression
Putative miRNA Targets on Ccng1
3'UTR of Ccng1
(miRNA target sites are highlighted)		 
>Ccng1|NM_009831|3'UTR
   1 TTGATAAATCTGGTTGTTATTCTCTGTATACAGAAAATTTTCCAGTATGATCATTTTCTGCTACAACTGAAGAATTGAAA
  81 TACTATCTTCAATATAAAGAATATGGGATGAAAACATAAAGGAAAAGTGAATTGTTGACTGGTCTAGATAGAGAATACTG
 161 GAAGGCATTCACTGTGTACAGTGCGTAGCAGTTTTAAGAGAAAAGACATATCAAACCCCTAGATATACGCTAATACTTTT
 241 CATCAAAAGATTAGCGTAGTAGCAAAGAGAATACTTTAAACTCGAATTTTAAAAGTAGTTACTGAAATAGCACTTCTTTA
 321 AATTACGTACCACCCCACTGTAGCTTATTTAAAGTTGCATAAGCCCATGCAGAACAACAAGCAATGTGACCCATATATGA
 401 ACAAATTTTAATCTGCCCATCGACTATGAAAATGAAGTACAAACCTGGGTGATGGACTTACAAAGTAATATAGGGCATGC
 481 CCATGTTAGGTTTCTGGAAACTGCCAGAGTGTCTTAATTCTATAGCTAGTATTTTACCTCTATAGCATTTGGACTAATAC
 561 AAAGTAATTATATGCATGAAAATATAAAATTGGTCTCTGATACATACACATTTTTGACATCTAAATTTCTTAATCATAGC
 641 AAAGATTTATCTTTTTATGATTAAAAACAATTTTTTCCTTAATGCATGGCAGCACATGCCTTTAATCCCAGCACTAGAGA
 721 GGCAGAGACAGGTAGATCTCTGAGTTCAAAGCCAGGCTGGTCTACACAGTGAGTTCCAAGACAGCTAGAGACCTTGTCTC
 801 AAAAACACTCAAAACAACCAAACACCAGGGGTAAATGTTGCTGGGAAGTCAGGGAAGATGGTTTAGAAGGGAAGCCTGAC
 881 AACCTGACTTCATTCTTCAGGACCCACATGATGGAAGGAGAGAACGAAGAACTCCCAAGTTCTCTCACATATGCACATAC
 961 CTCACCACCCCCCGCAGGAAATACATGATCATGCGTCTGAGATATCACCAGTTCACCTTTAGCAGCTCGCAGTTTGTAGG
1041 CAGATTTCTGTTAAGTTGGGTCTGTGTTGTTTGCCTATGTAGCAGGATTACAGCAGCAGCAAAAACGGTCCCTCAAGTCT
1121 TTCTGCCACTCTGACCTGAGTTTCCTACGGTACAGGATTTACTCTGAGAAACCTCAGCACCTTGCCACAGTAGCCTTGGC
1201 AGAATGGCCTCACGGTTAGGGAAACTCCTGATTCTAAGCTTGGGAGAGCTACGCTTAGATTTGAATTCACCCAGGAAGCA
1281 TTCAAATCAAGGCTAAAGACATAAATGTGAAATAAAACTGTGAACCTTCATTCTAAAGTTCATCTGACTTCCCAGATTTG
1361 ATCAATATATTCTTAGGTGGTATTAAAAATGGTAAACTGCTTAATTTAAATCTCAAAATTTAAATTATGAGGTTTACATA
1441 AAAACCAACATTTCATGAATGCACTTTTAAGGTATTAAAAGGGGTACTTAAGCGGTAAATGGTTTCTTGGCACCCATAAC
1521 CAAGTAATAGTTAATTTACAGGTGGGATTTTTTTTTATTGCTATGAGAATTACATTTAAAATTGTGGGTGTTTTATATAA
1601 AGCAGATATCACAAGTTTTGAAAATTTGTTACCTTTAATATTTCTTCTAGAGAATAGGTGTTTGTATCCATAATAAAAGA
1681 AAAATTTGTCAGAACTGCTGCTTCAATCTAATCCCATTTGAGAGAATTGTCTTTACTGTCTTAATAACTGGATGAATTAT
1761 CACTCTGAAAATGTATTTATTGCACTAAAGTTAGTTTAGGCTTGATAAAACACTCCAGACATTTTTACTACAGACTGTTT
1841 CTATAAAACTGCCATTGCTTCTAATGGAGAATTTTATTTTAAAAGAAATAAAATTGCTGAGTTTATCTGCAAAACCTTTC
1921 TCTAAGTCTTATGGGACGTAAGGAGACACCTCCATAATTATAAGAGCCGTTGTGCTCAGAGTCTCCATGCTGGCTTGAAT
2001 GTATGATCCACACTGTTAAAACATAGGCAGCAGCTCAGGGCCCTGGACAGCCTGAGTCAACCTAGAGTAGCTGGAACCAT
2081 TTTGACATGTAATGGATAAGAAAATTATCCATTGAGAAGCTGAACAATAAACCAAAGAACGGGTGTATTTTATCCTTAAC
2161 CTCTGTAAACCACGTTACACTGAGAACACTTCAGTTCTTCCTAAAGGTTGATAGGCTTCAGTCTGAAAACAATATTGATT
2241 TGGAGTGGACAGAAGTTAACTAACCAACTACCATTATGTTTTGAATACACCTTTCAATAAAATGATTGAAATGCAAAAAA
2321 AAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' guuugugguAACAG---UGUGAGGu 5'
                   ||| :   ||||||| 
Target 5' agtttaggcTTGATAAAACACTCCa 3'
 1793 - 1817 141.00 -13.00
2 
miRNA  3' guUUGUGGUAACAG------UGUGAGgu 5'
            :| ||| |||||      ||||||  
Target 5' taGAGACC-TTGTCTCAAAAACACTCaa 3'
 786 - 812 137.00 -16.30
3 
miRNA  3' guUUGUG----GUAAC-AGUGUGAGGu 5'
            | |||    || ||   |||||:| 
Target 5' aaACCACGTTACACTGAGAACACTTCa 3'
 2167 - 2193 130.00 -14.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Location of target site 3'UTR
Article - Esau C; Davis S; Murray SF; Yu XX; Pandey et al.
- Cell metabolism, 2006
Current understanding of microRNA (miRNA) biology is limited, and antisense oligonucleotide (ASO) inhibition of miRNAs is a powerful technique for their functionalization. To uncover the role of the liver-specific miR-122 in the adult liver, we inhibited it in mice with a 2'-O-methoxyethyl phosphorothioate ASO. miR-122 inhibition in normal mice resulted in reduced plasma cholesterol levels, increased hepatic fatty-acid oxidation, and a decrease in hepatic fatty-acid and cholesterol synthesis rates. Activation of the central metabolic sensor AMPK was also increased. miR-122 inhibition in a diet-induced obesity mouse model resulted in decreased plasma cholesterol levels and a significant improvement in liver steatosis, accompanied by reductions in several lipogenic genes. These results implicate miR-122 as a key regulator of cholesterol and fatty-acid metabolism in the adult liver and suggest that miR-122 may be an attractive therapeutic target for metabolic disease.
LinkOut: [PMID: 16459310]
86 mmu-miR-122-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003075 Tmem50b transmembrane protein 50B 1 1
MIRT003076 Lass6 ceramide synthase 6 1 1
MIRT003077 Gpx7 glutathione peroxidase 7 1 1
MIRT003113 Tgfbr1 transforming growth factor, beta receptor I 3 1
MIRT003114 Sbk1 SH3-binding kinase 1 3 1
MIRT003117 Hist1h1c histone cluster 1, H1c 3 1
MIRT003118 Ddc dopa decarboxylase 2 1
MIRT003120 Rell1 RELT-like 1 3 1
MIRT003121 Bach1 BTB and CNC homology 1, basic leucine zipper transcription factor 1 2 1
MIRT003122 Apob apolipoprotein B 2 1
MIRT003123 P4ha1 procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide 2 1
MIRT003124 Ccng1 cyclin G1 2 1
MIRT003125 Slc7a1 solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 3 3
MIRT003126 Gys1 glycogen synthase 1, muscle 2 2
MIRT003127 Slc35a4 solute carrier family 35, member A4 2 2
MIRT003128 Hfe2 hemochromatosis type 2 (juvenile) 3 3
MIRT003129 Tmed3 transmembrane p24 trafficking protein 3 2 2
MIRT003132 Bckdk branched chain ketoacid dehydrogenase kinase 4 1
MIRT003133 Aldoa aldolase A, fructose-bisphosphate 4 7
MIRT003134 Ndrg3 N-myc downstream regulated gene 3 4 2
MIRT003135 Cd320 CD320 antigen 4 1
MIRT003724 Smarcd1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 3 1
MIRT003725 Rcan1 regulator of calcineurin 1 2 1
MIRT003726 Gde1 glycerophosphodiester phosphodiesterase 1 2 1
MIRT004536 Ccrn4l nocturnin 4 1
MIRT005975 Klf6 Kruppel-like factor 6 4 1
MIRT005976 B2m beta-2 microglobulin 1 1
MIRT005977 Afp alpha fetoprotein 1 1
MIRT005978 Ccl2 chemokine (C-C motif) ligand 2 1 1
MIRT005979 Csf3r colony stimulating factor 3 receptor (granulocyte) 1 1
MIRT005980 Ctgf connective tissue growth factor 1 1
MIRT005981 Cxcl13 chemokine (C-X-C motif) ligand 13 1 1
MIRT005982 Cyp2b13 cytochrome P450, family 2, subfamily b, polypeptide 13 1 1
MIRT005983 Dbp D site albumin promoter binding protein 1 1
MIRT005984 Igf2 insulin-like growth factor 2 1 1
MIRT005985 Il1b interleukin 1 beta 1 1
MIRT005986 Jun jun proto-oncogene 1 1
MIRT005987 Per1 period circadian clock 1 1 1
MIRT005988 Alpl alkaline phosphatase, liver/bone/kidney 2 1
MIRT005989 Cs citrate synthase 2 1
MIRT005990 Prom1 prominin 1 3 1
MIRT005991 Sox4 SRY (sex determining region Y)-box 4 3 1
MIRT013527 Uros uroporphyrinogen III synthase 1 1
MIRT013528 Ppox protoporphyrinogen oxidase 1 1
MIRT013529 Urod uroporphyrinogen decarboxylase 1 1
MIRT013530 Cpox coproporphyrinogen oxidase 1 1
MIRT013531 Fech ferrochelatase 1 1
MIRT013532 Hamp hepcidin antimicrobial peptide 1 1
MIRT013533 Tfr2 transferrin receptor 2 1 1
MIRT013534 Smad4 SMAD family member 4 1 1
MIRT013535 Smad7 SMAD family member 7 1 1
MIRT013536 Bmpr1a bone morphogenetic protein receptor, type 1A 1 1
MIRT013537 Hba-a1 hemoglobin alpha, adult chain 1 2 1
MIRT013538 Hmbs hydroxymethylbilane synthase 2 1
MIRT013539 Mir17 microRNA 17 2 1
MIRT013540 Alas2 aminolevulinic acid synthase 2, erythroid 2 1
MIRT013541 Mir451 microRNA 451a 2 1
MIRT013542 Tfrc transferrin receptor