miRTarBase - #MIRT003791


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pre-miRNA Information
pre-miRNA mmu-mir-486a   
Genomic Coordinates chr8: 23142555 - 23142682
Description Mus musculus miR-486a stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-486a-5p
Sequence 33| UCCUGUACUGAGCUGCCCCGAG |54
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Pax7   
Synonyms Pax-7
Description paired box 7
Transcript NM_011039   
Expression
Putative miRNA Targets on Pax7
3'UTR of Pax7
(miRNA target sites are highlighted)		 
>Pax7|NM_011039|3'UTR
   1 GGTCCCTGGGGCAACTTGCCCCATCCAGTGGCCCAGCCAACCCTTCCCAAGCCCTGAGTCTCCTCACCTCAGTCCCCTTA
  81 TCCCCCTGGGGTTGCAGGAGACCAAGGGAAAAAAACCCTTTCCCTTCCTACAGGAAACCCTCTGGAGACGGAAAACCAGT
 161 GTGCCATCTACCCATGCTTAGTGACCCAGAGTGGCCCCTTGCCTTCCCCTCTTTCTTCAGAGGGGTTCCTAGGCATCCTG
 241 CAGTGACCTCCAGCTCACATCCACCTTCTCTGTATCGTGGCCTCGGTCCTGTCTCAGTGCAGAGATTGAGGCTCAATTTG
 321 AACCAAGCACCTAGTTATCAGAAGAAAATGGTGCCAAAGACAAGGCCCTGGAGTCCTTGACCTCTGAGTCGTGGGTGCCC
 401 TGGCTATGGGTGTAGGTGGAGCCCATGGGTGTCCTCAGTCACAGAGCTGGGAGCTCTCTCTCGCTCGCTTGGCATCAGGA
 481 CTGCAGCCTCTTTCACTGGACACTGAGATGAGTCCCCAGGGTGTTCCCAGGGGAGAAAGCAGGTAACATCCCAGCTTTAC
 561 CTAGGAATCCAGAGGACTTTAGGACTGTCCCTATGCACCCTGCAGGGCATCAGGAGACCAGGAAGGGATTCTAGCAGAGG
 641 GTAGGGGGCACAGAGGCAGAGCTGATTGCCCATGGGCTATCCCAGAATGCCTGGTCCTGAATCTAGCATCAGGAGGTGCA
 721 GGATTCCTAGGCTGCAATCTGACAGAGGCTTGCCCACTGTGTCAGGCCTGGGCAGCCCACAGAACCTGTCACTCTCCTCA
 801 ATTGGTAGGAGAAGAGGTCTTGAGGTGACAGGAGGCAGAAGGCAGGCTCAGACAGTCAGAGAGCACCAAGCTTTCAAGTC
 881 CGCACCCCTGGGGTTCGGCATACCATCTTGCTGGCAGCTGGAAACCTGGTTCCCTGAAAGGGGGCCTCCATCCTCCAGAA
 961 TGTAAGGCTCTTGATGCCACCGGATGCAGAGAGCCTTCTCGGGCCAGACAAAATTGCTGCTCCACCCCAGAGAAGATGTT
1041 CCAGCCTTCTTGGCATCTTAGAGGAAGGCCATGTCGCTGTCCTTTTCAGAGTAGCATATTTTTCAGTGATGGCTGCTCAG
1121 TCAGGAGGCTTCTGTCGCCTTACAAAGCACAGTGCGCTCTGGGCACTGTTTCTAAGCCACCCCATCCCACCCCCACCCCC
1201 GCCACCCCGGGGACAGAGGAAGATGCTAAAAGTCCCAGCAAAGAGGACAAAGCACCTTTCTTAAGCACTCCAGAGTCTTC
1281 CTTGTACCCCGCCCTCTCTTAGAGCTGGGTCTTTTGAGGGAAACGGATTGCTGAGCCCTCCCCCCCCCCCAATCCTCTCC
1361 TCTGTGGAGCTGTTTATCATCCTCTATTTATCAAAATCGCATCCATCTTTACCCTCTCCTTCGCTATAGCCTACTTCTGG
1441 ATCACCCTCATCCAGTGCTGGTACCCCACAGCACTAAATCCAGGAACCCTGGGCTTGATCACCTGTTGCCACCTGTACAC
1521 ATGAAACCACCTGCTGGCCCGGCCCATGTCTCCTGCCCTCAGCCAGCAAGACATTCCTAGAGAGAGGAACTATGGGCTTA
1601 AAAGCCCCAACTGACTTCCTTTTGCCTGGGGACCTGAACCGACAAGACACCAGGGACACTTGTCTACATGAACATGTGAC
1681 CAATGTACACCGATTTCTCATCTCTAGACCTATTATCTGAAGCCTGTCCCGGGCCATGACTAGAATGGCTTGTATCTGTG
1761 GTTTAGAGAAGTCTAATAATAACTGAGGGCAAACTGACTCTCTGGTAGCATGGAGCACCAGGCGGATGGAGCTCACCAGC
1841 TCTGTCCAGGTTTCAAAGGAGGAGACTGTTGGGCTCTTCAAGGTCTGGACAAGAGGAAAGCCACATTGCCCCCTTGGGAA
1921 CCCAGGTTCTCCTTTTGAACTTCTCACAGCTGCAAGCACCCCTTTCAAAGACCAAATGCATCCTCCTCCACATTCCTTGC
2001 TCCCTGGAGGCCTGGCTCTGGATACACCTGAGTCTTCGTTCACCTACTACACTTTAGGAGCAGGAACTTCAAGCAGGTGA
2081 CATCCACAGGGCCCAGTCCCAGCCAAGGGAGCAACATTCCAACGCTTGGACCAATCATAATGATCTGCCCGTGAGGGTAA
2161 CCGCAACTAGAGACCTGCTTGGGAGAAAACAAAATGACTTCTCATTCCATGCCATGCCTCTGAATGCTCCCCCAAGCTGC
2241 CATCTTGGTATAAAATGGGACTTGTGTTGTGGGGAACCCCTTGACCCCAACAGGTTTTCCCAACTGTCTCATGCTTTTGT
2321 GAATCTGTCTGCTTTGATCTGTAAAACTCAGCCTTGTTTGGGCAGCTTGTAATTTCAACAGTGAGGCGACATCGATTAGA
2401 TGAGAGGCACCAGGCCTCTCCGCCGCCGTCCCTCTGTGGCCGTCCCTCTGGGGTTGAGCAGAACCTAGAAGAAGGCCGAT
2481 TTCCAGTGGCCAGACTGGACCAGAAACAGCCCCCACCCCAATCCCTGTAAATAGAGTCAATAGCAAAATAAGAGGGGCGC
2561 CCTCCATGTCACCTCAAGTAGCTACTGGTTCTTCTGTGGAGGCCCCTCTGAACTCATTGTCTGGTAGTTGAAAATGTGAT
2641 GTTGTGCTGTTTGTTTATAGAACATTGGCTTTTTATATATAAATCTATATACTTAAAAACAAAAAC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gagccccGUCGAGUCAUGUCCu 5'
                 |||||| || |||| 
Target 5' agctcacCAGCTCTGTCCAGGt 3'
 1830 - 1851 135.00 -19.90
2 
miRNA  3' gagccccGUCG-AGU----CAUGUCCu 5'
                 :||| |||    ||:|||| 
Target 5' ctgaatcTAGCATCAGGAGGTGCAGGa 3'
 697 - 723 133.00 -15.40
3 
miRNA  3' gaGCCCCGUC-GAGUCAUGUCcu 5'
            |||  | | ||||||:|||  
Target 5' ctCGGTCCTGTCTCAGTGCAGag 3'
 282 - 304 132.00 -23.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12 , U2OS
Location of target site 3'UTR
Tools used in this research miRanda
Original Description (Extracted from the article) ... miR-206 and -486 are induced during myoblast differentiation and downregulate Pax7 by directly targeting its 3' untranslated region (UTR) ...

- Dey BK; Gagan J; Dutta A, 2011, Molecular and cellular biology.

Article - Dey BK; Gagan J; Dutta A
- Molecular and cellular biology, 2011
The Pax7 transcription factor is required for muscle satellite cell biogenesis and specification of the myogenic precursor lineage. Pax7 is expressed in proliferating myoblasts but is rapidly downregulated during differentiation. Here we report that miR-206 and -486 are induced during myoblast differentiation and downregulate Pax7 by directly targeting its 3' untranslated region (UTR). Expression of either of these microRNAs in myoblasts accelerates differentiation, whereas inhibition of these microRNAs causes persistence of Pax7 protein and delays differentiation. A microRNA-resistant form of Pax7 is sufficient to inhibit differentiation. Since both these microRNAs are induced by MyoD and since Pax7 promotes the expression of Id2, an inhibitor of MyoD, our results revealed a bistable switch that exists either in a Pax7-driven myoblast state or a MyoD-driven myotube state.
LinkOut: [PMID: 21041476]
16 mmu-miR-486a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003527 Pten phosphatase and tensin homolog 5 2
MIRT003791 Pax7 paired box 7 4 1
MIRT004586 Foxo1 forkhead box O1 5 1
MIRT578874 Fam129c family with sequence similarity 129, member C 1 1
MIRT581056 Shc4 SHC (Src homology 2 domain containing) family, member 4 1 3
MIRT590827 Zcchc9 zinc finger, CCHC domain containing 9 1 1
MIRT592961 Tns3 tensin 3 1 1
MIRT597071 Tbc1d8b TBC1 domain family, member 8B 1 1
MIRT598042 Nkain3 Na+/K+ transporting ATPase interacting 3 1 1
MIRT598358 Maoa monoamine oxidase A 1 1
MIRT598720 H2-Q4 histocompatibility 2, Q region locus 4 1 1
MIRT598800 Gm7609 predicted pseudogene 7609 1 1
MIRT599803 Aim2 absent in melanoma 2 1 1
MIRT601041 Cnnm3 cyclin M3 1 1
MIRT606321 Ms4a6c membrane-spanning 4-domains, subfamily A, member 6C 1 1
MIRT736452 Igf2bp3 insulin-like growth factor 2 mRNA binding protein 3 4 0

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