miRTarBase - #MIRT004280


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pre-miRNA Information
pre-miRNA mmu-mir-92a-1   
Genomic Coordinates chr14: 115044427 - 115044506
Description Mus musculus miR-92a-1 stem-loop
Comment The predominant miR-92 form cloned by Landgraf et al. has a additional 3' U residue, which is compatible with this precursor sequence, but not with that of mir-92-2 (MIR:MI0000580) .
RNA Secondary Structure
pre-miRNA mmu-mir-92a-2   
Genomic Coordinates chrX: 52741838 - 52741928
Description Mus musculus miR-92a-2 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-92a-3p
Sequence 50| UAUUGCACUUGUCCCGGCCUG |70
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
BE2BW7 miR-92a-3p Monitoring Biomarker (MOI) Clinical/Experimental Data Expression Increase Plasma Polymerase chain reaction
Gene Information
Gene Symbol Trp63   
Synonyms AI462811, Ket, P51/P63, P63, P73l, Tp63, Trp53rp1
Description transformation related protein 63
Transcript NM_001127260   
Other Transcripts NM_001127259 , NM_001127261 , NM_001127262 , NM_001127263 , NM_001127264 , NM_001127265 , NM_011641   
Expression
Putative miRNA Targets on Trp63
3'UTR of Trp63
(miRNA target sites are highlighted)		 
>Trp63|NM_001127260|3'UTR
   1 AGATCCCTGAACAGTTCCGACATGCCATCTGGAAGGGCATCCTGGACCACAGGCAGCTGCACGACTTCTCCTCACCTCCT
  81 CATCTCCTGAGGACCCCAAGTGGTGCCTCTACCGTCAGTGTGGGCTCCAGTGAGACCCGTGGTGAACGTGTGATCGATGC
 161 CGTGCGCTTTACCCTCCGCCAGACCATCTCTTTTCCACCCCGTGACGAGTGGAATGATTTCAACTTTGACATGGATTCTC
 241 GTCGCAACAAGCAGCAGCGTATCAAAGAGGAAGGAGAATGAGCGCCCATTGCGGGGTTCTTCCTGTCTTCTTCCACCTCC
 321 CAGCCCCTACAGGGCACGCCTGCTTGATCCTCAGAGCCTTCTCGTTAGCTCTTCTCCTTCTCCTTCTCAGTCTGGTTTCT
 401 AAAGGGACGGAGAATTAGGAGGCTGCCTGTTACCTAAAGTCTGACCTGTCACCTGATTCTGATTCTGGCTTTAAGCCTTC
 481 AATACTCTTGCTTGCAAGATGCATTGACATTGCTAGATAGAAGTTAGCAAAGAAGCAGTAGGTCTCTTTAAGCAGTGGAG
 561 ATCTCTCATTGACTTTTATAAAGCATTTTCAGCCTTATAGTCTAAGACTATATATATAAATATATAAATATCCGATATAT
 641 ATTTTGGGTGTGGGGGGTATTGAGTATTGTTTAAATGTAATTTAATGGAAATTGAGTTGCACTTATCATCCTTCTTTGGA
 721 ATTTGCTTGTTTCGGATGGCTGAGCTGTACTCCTTTCTCAGGGGTATCATGTATGGTGACAGATATCTAGAGTTGAATGG
 801 TCTATGTGAGTAACAATGACGTATAGGACCTCTCCTCATCCTTTGGATGGTTATTGTTTAGCACATCAAACCTGTGGATG
 881 CATCCAGTGTGTTTACCATTGCTTCCTATGAGGTAAAACTGTATATATGTACACAGTTTTCTCTGTCAGTATATTTTATG
 961 TTACTGGTGTCCATTCCAGTTAGGCTGGTTCACTCTGTGGCTATTACAAGCCACATTTTAGGTTTGCTTTGTCACACACT
1041 ATAAGACAGGGCATTGTCTCTTGCTTTTGTTTGAGAATGAGGAATGCAGTTGTGTTGTGGTTTGTTTTGTTTTATTTTGT
1121 TTTGTTTTCTGGAAACTCTTAAATGGTTCAAGTCAGCCATTCCAAATATCTGATGAAATTTAGCCCAATATAGCAGTAGC
1201 TCTTTGAAATTTAAGGCCCAACACCCTAGTATTTATTAGAAAAATAAACATTTGCTGTTGTTAGAATAGTCTTAAAAATA
1281 AATTTCTCTGCTAGATTGACTAAGTAAAATAGACATTCTCTGCTGTTGTGAGAATTTGGGCCAATTAGAATGAATGAAAT
1361 TCGTCTAGTTTTCATGGGGAGTTGTAATGTCTATTAGAAAGATTCAGGAAAAATAAGAATGATTCAGAAATACTGAATTT
1441 CCATGAAAAGGAAAACAGAAAGCGATTCATCCCACCAAACTCTGAATTGAAGTTCCTTTTGAAGGGTGGAGTGATGCTTG
1521 GGAAGTGGACCTTTTAAAGACTTTCCTATCTATGAGACACTGCATGCACAGGCAAGTTTCTCTCTCCCCAAGGGCTAAAA
1601 TAAGAATAATGGCTTGGAAAATACAAACTTCGTAGTGTAGTTTTCACATAGCATGAGCTGAACCACTGTTATCTTCCTCT
1681 TGATCATCAAAGCTTCATTGTTTTAGAAAGCAGAGGTGAAGACCCAGTTTTCCGCCTGACACTTTCCAAGCTAGTGTAGA
1761 CCAAGACCTGTCTACAAACCCACGACAAACCTTTTCACCTGTTTAATCCATATCCAGAAAGACTTGTTTCATACCTTGGG
1841 AAAGCATGCAACAGTATTCCCCTTAGATATTTTGGAAACATTTTGAGACAAGTATATTTTTTTTCCTGCCTAAACCAAGT
1921 GTTGTTTGTATGCTAATGAGCTCTACAATCTTCCCACACATTTTGTTAAATGACTTTCATTGCACATGAGCTCCCATTTT
2001 TTATTTTAAAGTGCAAATGGGCTAATAGGCCTTTGACGTGTAATGTATGAGTTTTGCCAGAAAATCATATCTTGTGTATA
2081 TGCGTGTGTGTGAAATTGCTTACTATGCTGGTTTTGTTTGTTATGGCTTTCTCTTTGGGATAGTTGGGTTTTCCAGAACC
2161 ACAGATGAAACTTTTTTTGTTGCTATTTTTATATTTTTGCAGAAACACCGTTTAGTGAGAATTCAATGTCAAATATGACA
2241 TGATACCTTAATTGTAAGAAGAAGGTGGGAAGGGAAAGTTGGTTTATTAATTTTTTTAAATTTTGTATGCAAAAGCAAAT
2321 GAGTCCTTAATTTCAACATTTTGTTGTGTTTAAATAATGATAAGCATCATTAACTTCTGTAACAAACTCACAGCTTTACA
2401 AATTCAATGGGTGGAGAAGAAAGCTGTGTCTTAGCCATGTTAGGAAGACAAATGGCTTCCTGTGTGTTGTAAGTATTTGG
2481 GCTGTTTCAGCAGTGTTGGTGTGGCACAGGGGACTCTGTGGCATTTCAGCACTATTTAGGTGGCACTAGGGACTCTGAAA
2561 TTCCTGTACTGTATCTGATGATTTTGGCATTAGCCATAGGTAGGCACAGTTTGTCTCCTCACACCAGTGTTTAGTGTGTG
2641 AATAGCCAGAGCTGTGGGGAAGAACACAGAGAACAGACATCTGCTGGATGCCTCTCAGTGGAGAATGGGATTCCTTCACT
2721 TGGTGGTGAAGCAGATAGGATAGAAAGCAGGATTCTCTTTGTTAATCCAGTTAGCTTTTGTTTTCTTGATATCCCCCCTG
2801 AATACGTTGAGTATGAGAGATATGTGGGTTTTTTTTATTTTTATAATTGTACAAAATTAAGCAAATATCAAATGTTTTAT
2881 ATACTTTATTAATGTTTTTTTTCAAAAGGTACTTTCTTATAGACATGATACTTTTTTACAGCTTCAGTTGCTTGTCTTCT
2961 GGTATTTTTGTGTTATGGGCTATGGTGAGCCAGAGGCAAATCTATAAGCCATTTTTGTTTGCCAGGACATGCAATAAAAT
3041 TTAAAAATAAATGAAAATACACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' guccGGCCCUGUUCACGUUAu 5'
              ||| |||:|||| ||| 
Target 5' caccCCGTGACGAGTGGAATg 3'
 196 - 216 141.00 -20.30
2 
miRNA  3' gucCGGCCCUGUUCACGUUAu 5'
             ||| |||||  |||||| 
Target 5' tttGCCAGGACA--TGCAATa 3'
 3018 - 3036 141.00 -17.00
3 
miRNA  3' guCCGG-CCCUGUUCACGUUAu 5'
            ||||   |||  |||:||| 
Target 5' taGGCCTTTGAC--GTGTAATg 3'
 2026 - 2045 135.00 -16.50
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions 32D
Location of target site 3'UTR
Tools used in this research miRanda
Original Description (Extracted from the article) ... Tbeta ...

- Manni I; Artuso S; Careccia S; Rizzo MG; et al., 2009, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Article - Manni I; Artuso S; Careccia S; Rizzo MG; et al.
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2009
MicroRNAs (miRs) are 21- to 23-nucleotide RNA molecules that regulate the stability or translational efficiency of target messenger RNAs of proteins involved in cell growth and apoptosis. miR-92 is part of the mir-17-92 cluster, which comprises members with an effect on cell proliferation. However, the role of miR-92 is unknown, and its targets have not been identified. Here, we describe a mechanism through which miR-92 contributes to regulate cell proliferation. Using a miR-92 synthetic double-strand oligonucleotide, we demonstrate that miR-92 increases 32D myeloid cell proliferation and 5-bromo-2-deoxyuridine (BrdU) incorporation and inhibits cell death. The effect is miR-92 specific since the miR-92 antagomir inhibits cell proliferation. Moreover, we show that miR-92 acts by modulating p63-isoform abundance through down-regulatation of endogenous DeltaNp63beta. Using luciferase reporters containing p63 3'UTR fragments with wild-type or mutant miR-92 complementary sites, we demonstrate that the wild-type 3'UTR is a direct target of miR-92. Finally, we observed that a miR-92-resistant DeltaNp63beta isoform (without 3'UTR) inhibits cell proliferation and parallels the effect of the antagomir. We conclude that one of the molecular mechanisms through which miR-92 increases cell proliferation is by negative regulation of an isoform of the cell-cycle regulator p63.
LinkOut: [PMID: 19608627]
24 mmu-miR-92a-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT002971 Hipk3 homeodomain interacting protein kinase 3 2 1
MIRT002978 Mylip myosin regulatory light chain interacting protein 3 1
MIRT004280 Trp63 transformation related protein 63 4 1
MIRT007244 Map2k4 mitogen-activated protein kinase kinase 4 1 1
MIRT053813 Zfpm2 zinc finger protein, multitype 2 3 1
MIRT054699 Tbx3 T-box 3 3 2
MIRT054792 Shox2 short stature homeobox 2 1 1
MIRT578185 Ncam2 neural cell adhesion molecule 2 1 1
MIRT580680 Stk10 serine/threonine kinase 10 1 3
MIRT583677 Fam136a family with sequence similarity 136, member A 1 1
MIRT584193 Cpeb1 cytoplasmic polyadenylation element binding protein 1 1 1
MIRT587067 Gm5148 predicted gene 5148 1 2
MIRT589036 Rhbdl3 rhomboid, veinlet-like 3 (Drosophila) 1 1
MIRT595174 Zpbp zona pellucida binding protein 1 1
MIRT595805 Chm choroidermia (RAB escort protein 1) 1 1
MIRT595903 Cd69 CD69 antigen 1 1
MIRT596292 Tagap1 T cell activation GTPase activating protein 1 1 1
MIRT596301 Tagap T cell activation Rho GTPase activating protein 1 1
MIRT596475 Btg2 B cell translocation gene 2, anti-proliferative 1 1
MIRT598598 Il5ra interleukin 5 receptor, alpha 1 1
MIRT598840 Gm14420 zinc finger protein 970 1 1
MIRT601409 Uba1y ubiquitin-activating enzyme, Chr Y 1 1
MIRT606063 Zfp300 zinc finger protein 300 1 1
MIRT735310 PTEN phosphatase and tensin homolog 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-92a Vorinostat (SAHA) approved 5311 Microarray A549 human non-small cell lung cancer cells 19513533 2009 down-regulated
miR-92a 5-Fluorouracil approved 3385 Microarray HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Microarray HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Quantitative real-time PCR HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Quantitative real-time PCR HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a Oxaliplatin (L-OHP) approved 5310940 Microarray HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a Oxaliplatin (L-OHP) approved 5310940 Quantitative real-time PCR HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a 3,3'-diindolylmethane (BR-DIM) NULL 3071 Quantitative real-time PCR Prostatic cancer 22412975 2012 down-regulated
miR-92a Isoflavone NULL 72304 Quantitative real-time PCR Prostatic cancer 22412975 2012 down-regulated
miR-92a Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 down-regulated
miR-92a Comfrey NULL 6440495 Microarray rat liver 21370286 2011 down-regulated
miR-92a Comfrey NULL 6440495 Quantitative real-time PCR rat liver 21370286 2011 down-regulated
miR-92a Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 down-regulated
miR-92a Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated

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