miRTarBase - #MIRT004532


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pre-miRNA Information
pre-miRNA mmu-mir-143   
Genomic Coordinates chr18: 61649196 - 61649258
Synonyms Mirn143, mmu-mir-143, Mir143
Description Mus musculus miR-143 stem-loop
Comment Expression of this miRNA in mouse was independently verified in heart and brain .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-143-3p
Sequence 40| UGAGAUGAAGCACUGUAGCUC |60
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B7G3SK miR-143 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Lower Blood Real-time polymerase chain reaction
B7G3SK miR-143 Predictive Biomarker (PRD); Safety Biomarker (SAF) Transcriptomic Data . . . .
Gene Information
Gene Symbol Vcan   
Synonyms 5430420N07Rik, 9430051N09, Cspg2, DPEAAE, NG2, PG-M, PG-M(V0), PG-M(V1), hdf
Description versican
Transcript NM_172955   
Other Transcripts NM_001134474 , NM_001134475 , NM_019389 , NM_001081249   
Expression
Putative miRNA Targets on Vcan
3'UTR of Vcan
(miRNA target sites are highlighted)		 
>Vcan|NM_172955|3'UTR
   1 GGTTCCAGTGGATGTCGGGATGGATTCTGTATTCTGTACTAAGTGCATGGAGGACCTACAGTGCAGTGACACAAACAGAC
  81 TTAATACCACCATTATGGTTAACACAGTAGGGAGTTTGGCAAGTGGGATTTTTTTTGGGAGGTGGGGAGATAACAACGTC
 161 AGAGTGGGGTCTTAACTGCACCCCAGAATTCCAAAAATCACAGAACACACCCTGAATTGAAATTTCTTATTAATTGAAGC
 241 CTTTCAATGCAATTTCACTCTTAATATAACAATGTTAAGACAACAGCAGCTGAGTTAAACCTTTGAATCCTGTTTTATTT
 321 TTTGATATGATTCTTATTATAAAAAGGAGACAATTTAAGCTTAAGTCATTGCAGGTGAGTATTTGTGCTGAAATTAATAA
 401 TGATTGTGTTCAATTCCTACCTAAAAATGCATCATACTATCAAATAATACGATTCACATATCTCATTTTGTTTCAAGTAA
 481 TAGAATAGGCGCCTTTTGGCGATTAATCTTGCTTTGAATTAATTAAAGTACAATACAGCCTCCTTGCATGAATTGGCTAT
 561 GCTCAGTTAGATTTGCCTTGTAAAATTAATTTTAGGTAGCATTCATTACATATATATATATGTATATATATATATTAGAC
 641 ATTCTTAAATTATTTTAAAATATTAGTCACATACAGTTTTATAGTTGTAGAGTGCTTAACTAAACTAATAACTAGTCAAT
 721 ATTTTATTGTAATATGTACTTTAGGATCGGTAAGATGTTAGCTAACAAATGCCTCCATACTTTAGCAACAGAGGCCAAGT
 801 CTGACATCGAGTATCAGAACAATGAAAAGCCAATGTAACACATGAAGTCTTTTAAACCAGCTTTCTAATTCCTTTTGTTA
 881 GATCAATGGGTAATTAAAATAGCTTGAAAATGTACTAGTTTGTTATTTATTAGTTTCTAAAGTGAGCTATGCAGGAATTT
 961 TTTTCAGCAGGAAAAAAGACAGATATGTAGGTAAACAGTGTGTTGGAAAAGATATTGGATCCATGAGCTAATAGAAGCAT
1041 GGAAAAGTATCATATCACAGACTTCCGTCTCCCTGCTAGGCTGCCTCTGCCTAAATCTCCACTTTACACTTGATTTTCTG
1121 AGGGTTATAGCCATATGTTGTGTATTAACTTAATTCAAGCTGGCCTGTTCATAAAAAGTCCTAGAGAAAGGTTTCTTCTC
1201 AGGCCTTGAAACAACAAAGTCTTCCATAGGTTTTGTTTTTTGTTTTTTTTTTTTTCAGAAAGACAATAATAATAATGAAA
1281 AATCTTATCCTAAATATAGTCATATATACATGTATACACATATAGCATGTATATTCATTTATTCCAAATTTCTCATTAGT
1361 GATATTCAAGTCAATTGGTTTATAGGATGACTTTTACATTAAGTTACTAACTTAAGGAAGGAACAGGCTTGGGTTGGTGG
1441 TGAGAAGGAGATGAGACAGAAAAAGTTAAAGGAAGGCTGAATAGAGTGGTTAGCCTGACTATCTTCATAAAATTTTCCTG
1521 TGCTGTATAAAATTAACAACAGAGAAAATTAAATAGCATATGGAAACCCAAGGTTTTCTGTAGCCATAGAGAATATTATT
1601 AAAGAGTTGATCCCGGCAGGCTTTAGGTTGTCTATGTAAATGAGTTCATATGGGTACATAACATAGCAAGGTCCTTAGGT
1681 TTCAAATATTGACCTCATTAAATTTAACTTACACAAGTGGTTTATATTTATTCTTTTTTTTAAAAAAATTCTTTTTATAG
1761 TCATTTAAATGTATAGTCTTTTGGAGTAAAATACATCCTGTGCCCGTGTAACCTGAAGTCAAGATTCTAGTTCATTACAT
1841 CCCTCCTTAAGACTATAAGTCCCAATTCATTCAGCACATAAGAAGTTAAGAATGTGGCAGAGCCAACAGCTTCCTTTACT
1921 TTCTATTGCATTTTGCAGGGGTTCTTTTAATACGGGAATCCATAAAGACTTTAAATGATTTCCAAATAGTATGACTGATG
2001 TCCAGCTTTGCCACATAGTCCAGTGGTGACTAAGCATGGCCTTATCTGCCGTTCCTAAAGTGATGTCAGTGATGTGCAGT
2081 TGTCTTGATAAATTGAAAAGCAAGAGATAAATGCATGCTGTAAATCATTTACCTTTGGCAAACATAGAAAGTGTATTTTT
2161 AGGCCACACCGTGAATAAACATTCCAAATCTT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cucGAUGUC---ACGAAGUAGAGu 5'
             ||| ||    |:||| |||| 
Target 5' gtcCTAGAGAAAGGTTTCTTCTCa 3'
 1178 - 1201 126.00 -14.20
2 
miRNA  3' cucGAU--GUCACGAAGUAGAgu 5'
             |||  ||  || |:||||  
Target 5' tgaCTAAGCATGGCCTTATCTgc 3'
 2027 - 2049 120.00 -7.60
3 
miRNA  3' cucgAUGUC--ACG---AAGUAGAgu 5'
              ||:||  ||:   |||||:|  
Target 5' cacaTATAGCATGTATATTCATTTat 3'
 1317 - 1342 119.00 -5.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions SMCs
Location of target site 3'UTR
Tools used in this research microRNA.org , miRBase Target Database
Original Description (Extracted from the article) ... In the current study we found that miR-143 can also bind to the versican 3'UTR at an evolutionarily conserved miR-143 binding site (figure 6) and suppress versican mRNA and protein expression (figure 5).//These data suggest that miR-143 modulates versican expression through the consensus miR-143 binding site in versican's 3'UTR. ...

- Wang X; Hu G; Zhou J, 2010, The Journal of biological chemistry.

Article - Wang X; Hu G; Zhou J
- The Journal of biological chemistry, 2010
Smooth muscle cells (SMCs) retain remarkable plasticity to undergo phenotypic modulation in which the expression of smooth muscle markers is markedly attenuated while conversely expression of extracellular matrix (ECM) is dramatically up-regulated. Myocardin is perhaps the most potent transcription factor for stimulating expression of smooth muscle-specific genes; little is known, however, about whether myocardin can orchestrate ECM expression to act in concert with smooth muscle differentiation program. In this study, we demonstrated myocardin coordinate smooth muscle differentiation by inducing transcription of microRNA-143 (miR-143), which attenuates ECM versican protein expression. Previous studies have shown that versican is a chondroitin sulfate proteoglycan of the ECM that is produced by synthetic SMCs and promotes SMC migration and proliferation. Our data demonstrated that myocardin significantly represses versican expression in multiple cell lines, and this occurs through the induction of miR-143 by myocardin. By a modified reverse transcribed PCR, we found that miR-143 specifically binds to the 3'-untranslated region of versican mRNA. Reporter assays validated that miR-143 targets versican 3'-untranslated region through an evolutionarily conserved miR-143 binding site. Furthermore, overexpression of miR-143 significantly represses versican expression, whereas conversely, depletion of endogenous miR-143 results in up-regulation of versican expression. In addition, we demonstrated that myocardin represses versican through induction of miR-143. Finally, we found that the regulation of versican by miR-143 is involved in platelet-derived growth factor BB-induced SMC migration. This study provides the first evidence that myocardin, in addition to activating smooth muscle-specific genes, regulates ECM expression through induction of microRNAs during smooth muscle differentiation.
LinkOut: [PMID: 20489207]
24 mmu-miR-143-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004492 Prkce protein kinase C, epsilon 4 1
MIRT004532 Vcan versican 4 1
MIRT004772 Elk1 ELK1, member of ETS oncogene family 4 1
MIRT006031 Kras Kirsten rat sarcoma viral oncogene homolog 5 1
MIRT006032 Rreb1 ras responsive element binding protein 1 5 1
MIRT006121 Ptn pleiotrophin 3 1
MIRT054755 Igfbp5 insulin-like growth factor binding protein 5 2 1
MIRT586427 Ogfrl1 opioid growth factor receptor-like 1 1 1
MIRT589192 Ptpn3 protein tyrosine phosphatase, non-receptor type 3 1 1
MIRT591160 Myo5c myosin VC 1 1
MIRT593574 Zyg11b zyg-ll family member B, cell cycle regulator 1 1
MIRT594328 Nav1 neuron navigator 1 1 1
MIRT595606 Mapk8 mitogen-activated protein kinase 8 1 1
MIRT596433 Crebzf CREB/ATF bZIP transcription factor 1 1
MIRT596597 Zfp488 zinc finger protein 488 1 1
MIRT599868 Acnat1 acyl-coenzyme A amino acid N-acyltransferase 1 1 1
MIRT600661 March5 membrane-associated ring finger (C3HC4) 5 1 1
MIRT602320 Gas2l3 growth arrest-specific 2 like 3 1 1
MIRT604071 Egln2 egl-9 family hypoxia-inducible factor 2 1 1
MIRT731148 Dnmt3a DNA methyltransferase 3A 1 1
MIRT733322 Sox2 SRY (sex determining region Y)-box 2 2 0
MIRT734441 Map3k7 mitogen-activated protein kinase kinase kinase 7 2 0
MIRT735453 COL5A2 collagen type V alpha 2 chain 1 0
MIRT756168 Npy neuropeptide Y 2 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-143 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 up-regulated
miR-143 Trichostatin A (TSA) NULL 444732 Quantitative real-time PCR myocardial differentiation of mouse ES cells 19521018 2009 down-regulated
miR-143 Sodium butyrate NULL 5222465 Quantitative real-time PCR Burkitt lymphoma cells 24577510 2014 up-regulated
miR-143 XMD8-92 NULL 46843772 Microarray pancreatic ductal adenocarcinoma cell 24880079 2014 up-regulated
miR-143 Medpor NULL NULL Microarray osteoblast-like cells line (MG-63) 18408260 2008 down-regulated
miR-143 17beta-estradiol (E2) approved 5757 Microarray MCF-7AKT breast cancer cells 19528081 2009 down-regulated
miR-143 Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-143 Trichostatin A (TSA) NULL 444732 Microarray apoptosis-resistant breast cancer cells 21971930 2011 up-regulated
miR-143 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-143 Estrogen NULL NULL Quantitative real-time PCR breast cancer cells. 22260523 2012 down-regulated
miR-143 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated
miR-143 Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 up-regulated
miR-143 Conjugated linoleic acid NULL NULL Quantitative real-time PCR retroperitoneal adipose tissue 20886002 2010 down-regulated
miR-143 Ursodeoxycholic acid (UDCA) approved 31401 Microarray rat Liver 20689055 2010 up-regulated
miR-143-3p Docosahexaenoic acid NULL 445580 Quantitative real-time PCR Caco-2 cells 24623846 2014 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-143-3p Doxorubicin 31703 NSC123127 approved sensitive Low Osteosarcoma tissue and cell line (SAOS-2, U-2-OS)

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