miRTarBase - #MIRT005315


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pre-miRNA Information
pre-miRNA mmu-mir-181b-1   
Genomic Coordinates chr1: 137966639 - 137966718
Description Mus musculus miR-181b-1 stem-loop
Comment Mouse miR-181b was predicted by computational methods using conservation with human and Fugu rubripes sequences .
RNA Secondary Structure
pre-miRNA mmu-mir-181b-2   
Genomic Coordinates chr2: 38853830 - 38853918
Synonyms Mirn181b-2, mir-181b-2, Mir181b-2
Description Mus musculus miR-181b-2 stem-loop
Comment Mouse miR-181b was predicted by computational methods using conservation with human and Fugu rubripes sequences .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-181b-5p
Sequence 12| AACAUUCAUUGCUGUCGGUGGGU |34
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
BK9AD8 miR-181b-5p Monitoring Biomarker (MOI) Clinical/Experimental Data Expression Decrease Peripheral blood Quantitative reverse transcription PCR
Gene Information
Gene Symbol Casp3   
Synonyms A830040C14Rik, AC-3, CASP-3, CC3, CPP-32, CPP32, Caspase-3, Lice, SCA-1, Yama, mldy
Description caspase 3
Transcript NM_009810   
Expression
Putative miRNA Targets on Casp3
3'UTR of Casp3
(miRNA target sites are highlighted)		 
>Casp3|NM_009810|3'UTR
   1 AGGAATGATTGGGGGTGGGGGGGGGCGTGTTTCTGTTTTGTTATGCCAAATGAGAAAGCTGTCAGGGAGACTCTCATTTA
  81 AATCTAATCTGACGGTCCTCCTGGTCTTTGTACGCTACCACTGCCTAGCAATGCAGCCAGCCACAGTGCAGCTACCTCAA
 161 CTTCGACATCAGGTAGTTGAAATGAAATTTAATTTAATAAGGAGCAAGTAACTGTCAATGATGGTACTATCATCCTAGAT
 241 GAAATTACAAAGTTGCCCTTTTATAATTAGCAAGATTTGGCGATACTATGAATTTTGAAGTCATTTTGAAGCAGTACAGC
 321 TTTTCCACTGATGATTTTATACTCCCCACTCATGTTAAGAATGTTGTTCTAGTTTTCGTTAAACGTAGAAACAATAATGT
 401 CAAATGATAATGTCTTAGAACTTGAATCCATGAGCAGAGTCAAAGGCTGGAACCCTTGTTTTGGACCTGATTTATGGAAG
 481 TGAAGAGTTGGACCACCATAGCATGCATTATAGCTACTGGTTTTGTGACAGTTGTCCACAACACGTATTTGTTATTTTAA
 561 AAAAAAAAATCATGTTTTATTACTGGAACAAAAATAACTTGAGTATTTATGTGAGAGGAAAACCGTGTGCGAACTTGGTT
 641 GACTCATTGAAGGCAAGGTGCTAAAATCCATCAGCATTTGTTTCTTACTTATCCTTATACAAATCAGCTCGGTCTTACAG
 721 ACCAGCAAAATATCTCAAGAGCTGTTATGTAACAGCCTCAAATGGTTGATTATTAGAATGAAGTACAGGAATTCTATAGG
 801 AATATACTATAATACCAGTTGTCAAACAGAGCTTAAGAAAAGGAGAGATGAAATCTACTGTAGTTGGAGAGAATGTGGTT
 881 TGAGCCAGAGCAGAGACTTGGCAGAGCCATGGGCACATCTTCAGAAAAGGCCTGCCAGCTGGACTCCCCACCCCTCACCC
 961 CACCCCCCTTGTGAGTTCTGGTTTGTGTGGATGATGTCCAAGTTACCACGTGGGAAAGTGAACCAGATCACAAACTTCTG
1041 CAAAAAGGCTTTCCCAGAGGGCTGAGAGTCTGAAGAAAGTTCACTGGACTCCATGTCCAGAGTCTGTAGCATGCTGACTT
1121 TTCTGTTGACGTTTACAAAGAAAGGAAAAATAATGGTGATATTTATACTGTGCTAGAAACGAAAGGGCCAGGGCACACAG
1201 GACTTGGAAAGCATCCAGCAATAGCCCTGCAGCCCATGTGGAGAAGCTGCGGGCTGCAGTCCATCCTCAGGGAGACTGTG
1281 CTCTGCTCCTGGCCTCCTTATGGGGGTGCTGAGTCACTGCTGGCTCTCGCAGCATTTCTCCTAAGCTTCCTTGTTGCACA
1361 AAGAAAGGAAGGAACTCACATTGTAACGGATAGATTCTTGTTTAGACTGGCTTTGTTGTTAACGCTTTCTAAGTGAATGC
1441 AGCATGTGATGGTATTTTAAAGTGTGTTCCCATGTGACAATTTTATAAAAGTTTGTTACCTTGCAATTTTATTTCAAAAC
1521 ATAAATTCAGATTTAAAATTATCAGTGTAT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugGGUGGCUGUCGUUACUUACAa 5'
            ||||: |: |: | |||||| 
Target 5' ccCCACTCAT-GTTAAGAATGTt 3'
 344 - 365 132.00 -16.00
2 
miRNA  3' ugggugGCUGUCGUUACUUACAa 5'
                |||:| | |||||| | 
Target 5' atttggCGATA-CTATGAATTTt 3'
 275 - 296 128.00 -9.10
3 
miRNA  3' ugGGUGGCUGUCG---UUACUUACAa 5'
            |:||:| :||:   |: |||||| 
Target 5' atCTACTG-TAGTTGGAGAGAATGTg 3'
 853 - 877 128.00 -13.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions mouse
Location of target site 3'UTR
Original Description (Extracted from the article) ... Most down-regulated genes with miR-181b seed sequences in activated B cells upon expression of miR-181b. (shown in Table I) ...

- de Yebenes VG; Belver L; Pisano DG; et al., 2008, The Journal of experimental medicine.

Article - de Yebenes VG; Belver L; Pisano DG; et al.
- The Journal of experimental medicine, 2008
Activated B cells reshape their primary antibody repertoire after antigen encounter by two molecular mechanisms: somatic hypermutation (SHM) and class switch recombination (CSR). SHM and CSR are initiated by activation-induced cytidine deaminase (AID) through the deamination of cytosine residues on the immunoglobulin loci, which leads to the generation of DNA mutations or double-strand break intermediates. As a bystander effect, endogenous AID levels can also promote the generation of chromosome translocations, suggesting that the fine tuning of AID expression may be critical to restrict B cell lymphomagenesis. To determine whether microRNAs (miRNAs) play a role in the regulation of AID expression, we performed a functional screening of an miRNA library and identified miRNAs that regulate CSR. One such miRNA, miR-181b, impairs CSR when expressed in activated B cells, and results in the down-regulation of AID mRNA and protein levels. We found that the AID 3' untranslated region contains multiple putative binding sequences for miR-181b and that these sequences can be directly targeted by miR-181b. Overall, our results provide evidence for a new regulatory mechanism that restricts AID activity and can therefore be relevant to prevent B cell malignant transformation.
LinkOut: [PMID: 18762567]
56 mmu-miR-181b-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004755 Timp3 tissue inhibitor of metalloproteinase 3 4 1
MIRT005296 Aicda activation-induced cytidine deaminase 5 1
MIRT005300 Slc35d3 solute carrier family 35, member D3 2 1
MIRT005301 Ak4 adenylate kinase 4 2 1
MIRT005302 Ndrg1 N-myc downstream regulated gene 1 2 1
MIRT005303 Pitpnc1 phosphatidylinositol transfer protein, cytoplasmic 1 2 1
MIRT005304 Tmem14a transmembrane protein 14A 2 1
MIRT005305 Cd244 CD244 natural killer cell receptor 2B4 2 1
MIRT005306 Gch1 GTP cyclohydrolase 1 2 1
MIRT005307 Errfi1 ERBB receptor feedback inhibitor 1 2 1
MIRT005308 Hk2 hexokinase 2 2 1
MIRT005309 Mxi1 MAX interactor 1, dimerization protein 2 1
MIRT005310 Ccdc58 coiled-coil domain containing 58 2 1
MIRT005311 Sc4mol methylsterol monoxygenase 1 2 1
MIRT005312 Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 2 1
MIRT005313 Pdxp pyridoxal (pyridoxine, vitamin B6) phosphatase 2 1
MIRT005314 Atp5g1 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9) 2 1
MIRT005315 Casp3 caspase 3 2 1
MIRT005316 Ulk3 unc-51-like kinase 3 2 1
MIRT005317 Snx25 sorting nexin 25 2 1
MIRT005318 Higd1a HIG1 domain family, member 1A 2 1
MIRT006312 Cbx7 chromobox 7 2 1
MIRT438370 SIX2 SIX homeobox 2 3 1
MIRT438687 Hspa5 heat shock protein 5 3 1
MIRT438688 Uchl1 ubiquitin carboxy-terminal hydrolase L1 3 1
MIRT577184 Smarca4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 1 5
MIRT577425 Tulp3 tubby-like protein 3 1 1
MIRT578325 Magea5 melanoma antigen, family A, 5 1 1
MIRT579346 Arhgef15 Rho guanine nucleotide exchange factor (GEF) 15 1 4
MIRT580671 Stk32a serine/threonine kinase 32A 1 1
MIRT580762 Spp1 secreted phosphoprotein 1 1 1
MIRT581071 Sgpp1 sphingosine-1-phosphate phosphatase 1 1 1
MIRT582047 Osbpl3 oxysterol binding protein-like 3 1 1
MIRT582656 Lnp lunapark, ER junction formation factor 1 2
MIRT583661 Fam160b2 family with sequence similarity 160, member B2 1 5
MIRT583691 Fam122b family with sequence similarity 122, member B 1 1
MIRT584580 Bnc2 basonuclin 2 1 1
MIRT584878 Ap1g1 adaptor protein complex AP-1, gamma 1 subunit 1 1
MIRT586224 Ptchd2 dispatched RND transporter family member 3 1 2
MIRT587223 Fam69c family with sequence similarity 69, member C 1 1
MIRT588616 Trim2 tripartite motif-containing 2 1 1
MIRT589131 Rad51d RAD51 paralog D 1 1
MIRT589794 Ikzf2 IKAROS family zinc finger 2 1 2
MIRT590593 Atp2a2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 1 1
MIRT591186 Ms4a4c membrane-spanning 4-domains, subfamily A, member 4C 1 3
MIRT591489 Aqp3 aquaporin 3 1 2
MIRT592858 Adamts9 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9 1 2
MIRT594856 Nacc1 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing 1 1
MIRT594983 Ebf3 early B cell factor 3 1 1
MIRT596592 Zfp689 zinc finger protein 689 1 1
MIRT598537 Itpk1 inositol 1,3,4-triphosphate 5/6 kinase 1 1
MIRT598728 H2-D1 histocompatibility 2, D region locus 1 1 1
MIRT600002 4930563E22Rik RIKEN cDNA 4930563E22 gene 1 1
MIRT603966 Gpd1l glycerol-3-phosphate dehydrogenase 1-like 1 1
MIRT605134 Clmn calmin 1 1
MIRT606009 Dagla diacylglycerol lipase, alpha 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-181b Teniposide approved 452548 Quantitative real-time PCR glioma cells 25151861 2014 up-regualted
miR-181b All-trans-retinoic acid (ATRA) approved 444795 Microarray acute promyelocytic leukemia 17260024 2008 down-regulated
miR-181b Curcumin NULL 969516 Microarray BxPC-3 human pancreatic carcinoma cell line 18347134 2008 up-regulated
miR-181b Trichostatin A (TSA) NULL 444732 Microarray human pancreatic cancer cell line BxPC-3 19112422 2009 down-regulated
miR-181b Doxorubicin approved 31703 Microarray ovarian cancer 19237188 2009 up-regulated
miR-181b Gemcitabine approved 60750 Microarray ovarian cancer 19237188 2009 up-regulated
miR-181b All-trans-retinoic acid (ATRA) approved 444795 Quantitative real-time PCR acute promyelocytic leukemia (APL) 19749800 2009 down-regulated
miR-181b Morphine approved 5288826 Microarray human monocyte-derived macrophages (h-mdms) infection with HIV-1 20564181 2010 down-regulated
miR-181b Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-181b Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-181b Ginsenoside Rh2 NULL 119307 Microarray human glioma cells U251 21372826 2011 up-regulated
miR-181b Aidi injection NULL NULL Microarray human breast cancer cells 21563499 2011 down-regulated
miR-181b Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-181b Olea europaea leaf extract NULL NULL Quantitative real-time PCR glioblastoma cells. 22722712 2012 up-regulated
miR-181b Temozolomide approved 5394 Quantitative real-time PCR glioblastoma cells. 22722712 2012 up-regulated
miR-181b Celecoxib approved 2662 Microarray gastric cancer cells. 23001726 2013 up-regulated
miR-181b 17beta-estradiol (E2) approved 5757 Microarray rat breast 17700064 2007 up-regulated
miR-181b Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-181b Estrogen NULL NULL Microarray mouse uterus 20720260 2010 down-regulated
miR-181b Estrogen NULL NULL Quantitative real-time PCR mouse uterus 20720260 2010 down-regulated
miR-181b Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 down-regulated
miR-181b Nicotine approved 89594 Microarray Rat adrenal pheochromocytoma PC12 cell 18845019 2009 up-regulated
miR-181b Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated
miR-181b Perfluorooctane sulfonate NULL 74483 Quantitative real-time PCR zebrafish embryos 20878907 2011 down-regulated
miR-181b Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-181b-5p Gemcitabine 60750 NSC613327 approved sensitive Low Pancreatic Ductal Adenocarcinoma tissue and cell line (SW1990, CFPAC-1)

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