miRTarBase - #MIRT005946


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pre-miRNA Information
pre-miRNA mmu-mir-200c   
Genomic Coordinates chr6: 124718322 - 124718390
Description Mus musculus miR-200c stem-loop
Comment Mouse mir-200c is predicted .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-200c-3p
Sequence 45| UAAUACUGCCGGGUAAUGAUGGA |67
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B0Q8FA miR-200c Safety Biomarker (SAF) Clinical/Experimental Data Expression Higher Urine Quantitative polymerase chain reaction
Gene Information
Gene Symbol Flt1   
Synonyms AI323757, Flt-1, VEGFR-1, VEGFR1, sFlt1
Description FMS-like tyrosine kinase 1
Transcript NM_010228   
Expression
Putative miRNA Targets on Flt1
3'UTR of Flt1
(miRNA target sites are highlighted)		 
>Flt1|NM_010228|3'UTR
   1 AGCTTCTCACCAGCCCCGACAACCAGCCCCTGACAGTATTATACATCTATGAGTTTACACCTATTCCGCTCCACAGGAGC
  81 CAGCTGCTTTTCGTGACCTTTAATCGTGCTTTTTTGTTTTTTGTTTTGTTTGTTGTTGCTGTTTTGACTAACAAGAATGT
 161 AACCCCAGTTAGTGACGTGTGAAGAATACTATTGTTAGAGAAATCCCCCCCGCAAAGCCTCAGGGTAACCTGGACAGGAA
 241 GGAGCAGGTGCCTCTGGCGACCGCCCCGCCCACCGGCCATGGCCCCACCCACCCTCCCTGCAGCTGTGGGACTAGAGGCA
 321 GTAAGCCCATTAGCTCATGGCTGCATGCACTGACCTGCTCTGTCTCTCTTATGGAGGAAAGGGAGAACAGAGCAAACAGG
 401 AGGCACAGGAAAAGGCTTTGGGATGCGTCCGTCCTGTGGAGCCCGTGCAGGAGGGGGCTCCGCTATGCCACTTCAGTGAC
 481 TTCTCACTCCTGGCCTCCGCTGTTTCGGGCCCCCTTCCAAGAGGTATCAGAGCAGAACATGAGGGACGTTTCCTAGACCA
 561 GGGCACATGTTCTCGGGAACCACAGTTAATCTTAAATCTTTTCCCGGGAGTCTTCTGTTGTCTGTTTACCATCCAAAGCA
 641 TATTTAACATGTGTCAGTGGGGGTGGCGCTTGGCTTCTGAGGCCAGAGCCATCATCAGTTCCTCTAGTGAGATGCATTGA
 721 GGTCATACCCAAGCTTGCAGGCCTGACCTTCGCATACTGCTCACGGGGAGTTAAGTGGTCCAGTTTGGCCTAGTAAGGTT
 801 GCCTACTGATGGGCTCAAAAGCCACATTTTAAACAGGTTTTATCTCAAGTATTAATATATAGACAAGACACTTATGCATT
 881 ATCCTGTTTTATATATCCAATGAATATAACTGGGGCGAGTTAAGAGTCATGGTCTAGAAAAGGGGTTTCTCTGTACCCAA
 961 ATCGGGCTGGTTGGACCAAGACCCAGAGAGGCAGAGTGGTTGTCCCAGCTATAGTTACTAAACTACTCACCCAAAGTTGG
1041 GACCTCACTGGCTTCTCTTTACTTCATCATGGATTTCACCATCCCAAGGCAGTCTGAGAGGAGCTAAAGAGTATCAGCCC
1121 ATATTTATTAAGCACTTTATGCTCCTTGGCACAGCAGGTGATGTGTAATTTATGCAAGCTCCCTCTCCAGCTAGGACTCA
1201 GGATATTAGTCAATGAGCCATCAAAAGGAAAAAAAAAAAACCATCTTATTTTCATCTGTTTCATACCTTGTCTGGGGTCT
1281 AATGACGATGGCAACAGGGTAGACATGGGAAGACAGGGTAGAAAAGGGTGCCCGCTCTTTGGGGTCTAGAGATGAGCCCT
1361 GGGTCTCTAAAATGGCTCTCTTAGAAGTTGTATGTGCAAATTATGGTCTGTGTGCTTAGGTCGTGCACACCTGCCGGAGC
1441 CGGTCACAGCTGGGCAGACGATGAATAGCTGCTTTGGGAGAGCAGAGCATGCTAGCCACTTAATTCTCTGACCGGGCCAG
1521 CATCATGGGTACCTGCTCCCCTGTGTACCCCATCCTTAAGGTTTTCTGTCTGATGAGACTGGAGGCCCAGTGCAATCCCC
1601 ACTGAGACAGCCTGCAGCCCACTGTGGCTCTTGGTGCACTCACCAGCCAGGACTAGACAAGTAGGAAAGGGCTTCTAGCC
1681 ACACTGGAGAAAAAGAAAATCAGGTAGGGCTGGCCAAAGACATCTTTGTCCATTCGCAAAAGCTCTTGTCGGCTGCAGTG
1761 TGTAAGTCAGGCGATGAGACAGAGGCTACCAGAGAAACGGATGAGAACAGCAGCCTGAGGTTTCTCATCCAGATATCCAG
1841 CAATTGGGGGGTGGGGGAAGACCATAGATGGTCCTGTATTATTCCGATTTTAATAATCTAATTCGTGATCATTAAGAGAC
1921 TTTAGTAAATGTCCCTTTCCCACAAAAGTAAAGAAAAGCTATCGGGATTCTCTGGTTCTGCTTAAAGACTTAGCTTTGGA
2001 GCCTATGAAAGTTGATCAGCCAGG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aggUAGUAAUGGGCCGUCAUAAu 5'
             | ||   ||:| ||||||| 
Target 5' acaACCAGCCCCTGACAGTATTa 3'
 19 - 41 156.00 -16.70
2 
miRNA  3' agguAGUAAUGG--GCCGUCAUAau 5'
              || ||::|  | |||||:|  
Target 5' aagcTC-TTGTCGGCTGCAGTGTgt 3'
 1740 - 1763 120.00 -9.60
3 
miRNA  3' aggUAGUAAUGGGCCGUCAUAAu 5'
             ||| || ||||| ||| || 
Target 5' taaATCTTTTCCCGGGAGTCTTc 3'
 593 - 615 120.00 -15.16
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions 393P , 344SQ
Disease 0.0
Location of target site 3'UTR
Tools used in this research microRNA.org , TargetScan
Original Description (Extracted from the article) ... miR-200b ...

- Roybal JD; Zang Y; Ahn YH; Yang Y; Gibbons et al., 2011, Molecular cancer research : MCR.

Article - Roybal JD; Zang Y; Ahn YH; Yang Y; Gibbons et al.
- Molecular cancer research : MCR, 2011
The microRNA-200 (miR-200) family is part of a gene expression signature that predicts poor prognosis in lung cancer patients. In a mouse model of K-ras/p53-mutant lung adenocarcinoma, miR-200 levels are suppressed in metastasis-prone tumor cells, and forced miR-200 expression inhibits tumor growth and metastasis, but the miR-200 target genes that drive lung tumorigenesis have not been fully elucidated. Here, we scanned the genome for putative miR-200 binding sites and found them in the 3'-untranslated region (3'-UTR) of 35 genes that are amplified in human cancer. Mining of a database of resected human lung adenocarcinomas revealed that the levels of one of these genes, Flt1/VEGFR1, correlate inversely with duration of survival. Forced miR-200 expression suppressed Flt1 levels in metastasis-prone lung adenocarcinoma cells derived from K-ras/p53-mutant mice, and negatively regulated the Flt1 3'-UTR in reporter assays. Cancer-associated fibroblasts (CAFs) isolated from murine lung adenocarcinomas secreted abundant VEGF and enhanced tumor cell invasion in coculture studies. CAF-induced tumor cell invasion was abrogated by VEGF neutralization or Flt1 knockdown in tumor cells. Flt1 knockdown decreased the growth and metastasis of tumor cells in syngeneic mice. We conclude that miR-200 suppresses lung tumorigenesis by targeting Flt1.
LinkOut: [PMID: 21115742]
33 mmu-miR-200c-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT002336 Zeb1 zinc finger E-box binding homeobox 1 4 3
MIRT002337 Zeb2 zinc finger E-box binding homeobox 2 3 4
MIRT005946 Flt1 FMS-like tyrosine kinase 1 1 1
MIRT006426 Mapk14 mitogen-activated protein kinase 14 2 1
MIRT009810 ZEB1 zinc finger E-box binding homeobox 1 1 1
MIRT009811 ZEB2 zinc finger E-box binding homeobox 2 1 1
MIRT053235 Sox2 SRY (sex determining region Y)-box 2 4 1
MIRT418784 Map2 microtubule-associated protein 2 1 1
MIRT438019 Bmi1 Bmi1 polycomb ring finger oncogene 2 1
MIRT438582 Zfpm2 zinc finger protein, multitype 2 2 1
MIRT438658 Nog noggin 5 1
MIRT577097 Fhod1 formin homology 2 domain containing 1 1 3
MIRT577625 St3gal2 ST3 beta-galactoside alpha-2,3-sialyltransferase 2 1 1
MIRT578593 Hist1h1d histone cluster 1, H1d 1 4
MIRT579059 Cradd CASP2 and RIPK1 domain containing adaptor with death domain 1 1
MIRT581140 Senp5 SUMO/sentrin specific peptidase 5 1 2
MIRT581298 Rnf166 ring finger protein 166 1 1
MIRT581364 Rdh10 retinol dehydrogenase 10 (all-trans) 1 1
MIRT581743 Plxnc1 plexin C1 1 1
MIRT581831 Pkib protein kinase inhibitor beta, cAMP dependent, testis specific 1 1
MIRT582081 Ogfod1 2-oxoglutarate and iron-dependent oxygenase domain containing 1 1 2
MIRT582414 Mphosph9 M-phase phosphoprotein 9 1 2
MIRT582457 Mgat3 mannoside acetylglucosaminyltransferase 3 1 1
MIRT582940 Ikzf5 IKAROS family zinc finger 5 1 1
MIRT583984 Ddx26b integrator complex subunit 6 like 1 1
MIRT595499 Mtf2 metal response element binding transcription factor 2 1 1
MIRT595530 Foxn3 forkhead box N3 1 1
MIRT596310 Rassf10 Ras association (RalGDS/AF-6) domain family (N-terminal) member 10 1 1
MIRT596609 Zfp459 zinc finger protein 459 1 1
MIRT601132 Bri3bp Bri3 binding protein 1 1
MIRT603385 Sh2d4a SH2 domain containing 4A 1 1
MIRT731202 Reln reelin 2 1
MIRT756395 Cd274 CD274 antigen 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-200c All-trans-retinoic acid (ATRA) approved 444796 Quantitative real-time PCR breast cancer cells 23904188 2013 up-regualted
miR-200c Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated
miR-200c Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 up-regulated
miR-200c Trichostatin A (TSA) NULL 444732 Microarray human pancreatic cancer cell line BxPC-3 19112422 2009 up-regulated
miR-200c Trichostatin A (TSA) NULL 444732 Northern blot human pancreatic cancer cell line BxPC-3 19112422 2009 up-regulated
miR-200c 17beta-estradiol (E2) approved 5757 Microarray MCF-7 breast cancer cells 19528081 2009 up-regulated
miR-200c Activin A NULL 229455 Quantitative real-time PCR Human embryonic stem (hES) cells 19885849 2010 up-regulated
miR-200c 5-Fluorouracil approved 3385 Microarray HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-200c CDF(analogues of curcumin) NULL NULL Quantitative real-time PCR pancreatic cancer (gemcitabine-sensitive) BxPC-3 cells and gemcitabine-resistant MIAPaCa-E (relatively resistant to gemcitabine) and MIAPaCa-M (highly resistant to gemcitabine) 20388782 2010 up-regulated
miR-200c CDF(analogues of curcumin) + gemcitabine NULL NULL Quantitative real-time PCR pancreatic cancer (gemcitabine-sensitive) BxPC-3 cells and gemcitabine-resistant MIAPaCa-E (relatively resistant to gemcitabine) and MIAPaCa-M (highly resistant to gemcitabine) 20388782 2010 up-regulated
miR-200c 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 up-regulated
miR-200c 1,3-bis(2 chloroethyl)-1-nitrosourea (BCNU) NULL 2578 Quantitative real-time PCR Human umbilical vein endothelial cells 21527937 2011 up-regulated
miR-200c Hydrogen peroxide (H2O2) NULL 784 Quantitative real-time PCR Human umbilical vein endothelial cells 21527937 2011 up-regulated
miR-200c DNA methyltransferases (DNMTs) + 5-azacytidine (5-AzaC) NULL NULL Quantitative real-time PCR umbilical cord blood-derived multipotent stem cells(hUCB-MSCs) 21572997 2011 up-regulated
miR-200c Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-200c Metformin approved 4091 Quantitative real-time PCR pancreatic cancer cells 22086681 2012 up-regulated
miR-200c Metformin approved 4091 Quantitative real-time PCR pancreatic cancer cells 22086681 2012 up-regulated
miR-200c CDF(analogues of curcumin) NULL NULL Quantitative real-time PCR pancreatic cancer cells 22108826 2012 up-regulated
miR-200c Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 up-regulated
miR-200c Paclitaxel + Cyclopamine NULL NULL Quantitative real-time PCR prostate cancer cell 22768203 2012 up-regulated
miR-200c Catechin approved 9064 Microarray apoE−/− mice 22253797 2012 up-regulated
miR-200c Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-200c Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 up-regulated
miR-200c Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated
miR-200c Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 down-regulated
miR-200c Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 up-regulated
miR-200c Curcumin NULL 969516 Quantitative real-time PCR Y79 RB cells. 22510010 2012 up-regulated
miR-200c Furan NULL 8029 Quantitative real-time PCR liver 22079235 2011 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-200c-3p Paclitaxel 36314 NSC125973 approved sensitive Low Ovarian Cancer tissue and cell line (HeyTGL)
mmu-miR-200c-3p Docetaxel 148124 NSC628503 approved sensitive Low Gastric Cancer tissue and cell line (BGC-823)
mmu-miR-200c-3p Erlotinib 176870 NSC718781 approved resistant Low Non-Small Cell Lung Cancer tissue and cell line (PC-9, ErlR)
mmu-miR-200c-3p Trastuzumab sensitive Low Breast Cancer tissue and cell line (SKBR3)

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