miRTarBase - #MIRT005975


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pre-miRNA Information
pre-miRNA mmu-mir-122   
Genomic Coordinates chr18: 65248861 - 65248926
Synonyms Mirn122a, mmu-mir-122, Mir122a
Description Mus musculus miR-122 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-122-5p
Sequence 6| UGGAGUGUGACAAUGGUGUUUG |27
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Plasma Microarrays; Real-time quantitative RT-PCR analysis
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Decrease Liver tissue Microarrays; Real-time quantitative RT-PCR analysis
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Decrease Liver tissue Quantitative real-time reverse transcription PCR
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Plasma Quantitative real-time reverse transcription PCR
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Plasma Quantitative reverse transcription PCR
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data expression Increase Serum Quantitative reverse transcription PCR
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression . Blood Polymerase chain reaction
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Serum Quantitative polymerase chain reaction
BOL4H5 miR-122 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Higher Blood Polymerase chain reaction
Gene Information
Gene Symbol Klf6   
Synonyms AI448727, BCD1, C86813, CPBP, Copeb, FM2, FM6, Ierepo1, Ierepo3, R75280, Zf9
Description Kruppel-like factor 6
Transcript NM_011803   
Expression
Putative miRNA Targets on Klf6
3'UTR of Klf6
(miRNA target sites are highlighted)		 
>Klf6|NM_011803|3'UTR
   1 AGGAGCAGAGGGACGAATCCTGTAGGCTAAAAGAGGCTTCCAGGCTAAGAGGCGGCCATGGAAGGAGGGATGCCTGTAAC
  81 AGCCAAAGCATGCCATTTTGCTTCCTATCCAGTTACCTCCAGGGGCCTCTCTTTGGAAGGTCTTTTGAGGGCTACAAAAG
 161 TCATGTCAGGAGTGGCATAGCACCCATGGTGCATGGTGTTTGGGTGACCCCGGACTCACCACTGGTTCCTAACCTTCTGA
 241 GAGGCTCTAAGCTTTTGGCCGTGAGCATGCGCACTGAGAATGTTAGTGGGTGGGATGGTTGTGTTGAGGATCTATTACTG
 321 ACTGTATGGTGAGGCAGACTTTTTTTTTCTCCCCCTATGTGGTATCAAATAACTGCAAGAGACAGAAAAAAAAAAAAGCA
 401 GTTTGAATGTTTTGTGTGTGAGGAGTATACCAAGGGATGAGTTGACCACCAATCATTTCCTGAAGGGTGTCTGCACCTTA
 481 GAAAAAAATAAACAAAAACAACAACAAAAAAATAAATAAAAGTTAAAATAATAAAAAAAAAGATGGAAAAACATGGAGGG
 561 TGGGAGCGGGAACTCAGGACCCATTGTGGTGAGTGTTAAGGGCAGGGGGAGCCCCTGTGCCCCTTTCCTGTGTGAAAGGA
 641 CTTTCTGGTGTCTGGTGCAGCTATAGAATGTGCAGTGTGTCAAGTAGCTTGTTTTACTGGCTACATGGAGCTCATTTGTA
 721 ACCCATTGTATAAGCTGTATTTACAAATCGCAGTGGTCATTTTCCCTTATAATACAAAAAAACAAGTCGTGCATGGTCTG
 801 GGGGAGCTATAAACGTTTAACATTTTTAAATTGCGTCTCCAATCTTGATTCCAATTAATAAGCAGCTATAAGATCTAATT
 881 TGTGTAGAATAATGATTCCTTAAGCCATTTAGGCCTGGCAAGACACGTTCTCCCCCCTCCCCCCCCCAGATCCTTCTATT
 961 TTGTAACACTCCCATGCAAAATGATCTTGTGCAATGTAGGGTTGTATTTTCGACGACTCGGAATTTGAATTTACTTTTTT
1041 TTTGAGAATCTTAAGGTACCCTTTGGAGAAATTGGACACTTGTCAAATGTCTAAATGCTAGGGGCCTGGAAATTACCCGT
1121 CTAATATCACATTAGGCTAAGAACACAAACTTTTTTTCTCAGAGTGGATCAAACCACTAAGCTTAGAAACCGTTTTTTCT
1201 CACGCAGCTACGTCTCTTATTTATGCCTTGAGGACTATTTCTGGTTTTCTAGCTGTTAATGCACTGTTGACCTTCATAAT
1281 GGTGCCTTATGCAACTGGCCCTTCTGTGGGGTCAAATACAGGGGAATGGGTGACGCATGCTTAATTAAGGCTCTTTTTTC
1361 ACCTGGCATTGTACTGTATCAATGTATAATATGGACAAAACAAACAAAGAACAACAACGACAACAAAAAGAACACCTCTT
1441 TAAGATCCTCCACAGTGATGAACGAACGGGTGAAAAGTCCTTTGACATGTCAACATGTGTTCATTTCCAACAACTTGACT
1521 GTCAGTGTTAAAATGGGGAAAAAAATAGACATGGCAATTGAAAACTAATTCTGCTACCATGAGACTCTTTTAAGAGACCT
1601 TGCACAACTGTACAGATCTCACATACCCACACACACCCACACACACATACACACACACACACACCGGCTGTATTTATTAT
1681 GTAACATTGTCACACATATTAAAGATACAGAAGTATTAAACAAACAAGAAAAACCAATGTCAATATCTTGCTCAAAAGGC
1761 ACAAGTTTTGAGTACCTGTCTAGCTATCTGTTGGTAATACTGAAAGTATACTGCTTTTTGTTTTGTTTTTGTTTTTTTTG
1841 AAAAAGTGGATGAAATTTTTTGTGTATGTATATTTGTGTGTATAGTATGTGTATGTATGTGTGTATATATATATTATATA
1921 TAGATAATATATAAATATTTTTTTAAGAAGAAACTAGAATGTTTAGCTAGAAAATTCTACAGCCTGTGAAGAAGTATTTC
2001 AAAATGGCCATAAAGGAGGTTAAAAGAAATTGAAAACTATAACCTAACTTTTATAGAGGCTTTATCTTTAATTTAATAGT
2081 TTGCTGGAGACATTGTTGGCGTTGAGTCTTTTCCAGGCTGCTGATTCTGCTAGTCACGATTGGCAAGCCTGGAATAGGGG
2161 ACCTTCGGCCATTTAGAAGGGGCATGAACAAAATACTGGCCAACCAGCTGGACTCAGACACACAAAGGTTTTAATTTTCC
2241 TTTCAGCCTTACTAGAAGATCTAACCTAAGAGTAAGCTGACCACAGGGATTGTTTTTTAGAATACTTTTTATGCAGATGA
2321 AACTATTTTTTCCAGCATGTAGATTCTTCCAGCTTTTTCAAGGAGTACTTTCCCGGAATTTGGCATACCACAGCGTAGAC
2401 AGCTGATATTTCTGATATTTCATCCAGCTGCTTGGCTTTTTGGGTGTGGATCTTTACATATATGAGAATGAGTCTGGCTG
2481 GGCTGGTATTTCGTTTGATCTTCCTGGAAACGAGCAATGATTAAAGCTCCCTTAACTGCTTTTTGTTTTGTTTTGTTTTA
2561 TTGTATCTGGCCTGATGAATACATTTCTCTACAGAACTGATTTCATTTTGCTCAAGGGGAAAATGCACTTTTGTTTGGCA
2641 ATTCAAAATCCTAGCACTTGATTTGCTGGATTTTGGAAAACTCCTTTTTTGGCCCTGCTGTGTGCTTATCCATAGCAATT
2721 CCATTAAGTAAGAAGGTAAACAGAAGAGAGAGGAAACTTGTGCTGGCGCGTCTCACTTATGAAACACAGCGACGTTGCTG
2801 GACTGGCTGGGGCTGGGTGCCGTCCGTGTCGATGCTGTAACTTTAATACTAAGCAGTCCATTCTGGTCAGGTGACAACTG
2881 AGCCTCAATCAAGCAGGAACTTTTGCTTGAAATTAAAAAAAAAAATTTCTATTAGTGAAATTTCTTTATTTCTTTTTTTT
2961 TGAAGCACCCTGTTATCTAAAGAATCTTTGTAAGATTTTTGTAAAATTTTGTTTTACAAGATTTTATTTGAAATTGTTTT
3041 TTGCGAGATTGTTATATTTCTGTATGAATGTATTTTTTATTCGAATAACATAAAAAGAATTCTTATCAGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' guuuguGGU--AACAGUGUGAGGu 5'
                |:|  |||| ||||||| 
Target 5' atccttCTATTTTGTAACACTCCc 3'
 950 - 973 158.00 -13.00
2 
miRNA  3' guuUGUG-GUAACAGUGUGAGGu 5'
             |||| ||    ||||| || 
Target 5' tacACACACA----CACACACCg 3'
 1648 - 1666 121.00 -13.40
3 
miRNA  3' guuUGUGGUAACAGUGUGaggu 5'
             ::| |||||||||||    
Target 5' tatGTAACATTGTCACACatat 3'
 1678 - 1699 119.00 -15.26
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions KO miR-122 mice
Disease 0.0
Location of target site 3'UTR
Tools used in this research miRanda , PITA , RNAhybrid , TargetScanS
Original Description (Extracted from the article) ... "Klf6 is a miR-122a target gene and contributes to hepatic fibrogenesis.//Five genes that we believe to be novel miR-122a targets were experimentally verified ...

- Tsai WC; Hsu SD; Hsu CS; Lai TC; Chen SJ; et al., 2012, The Journal of clinical investigation.

Article - Tsai WC; Hsu SD; Hsu CS; Lai TC; Chen SJ; et al.
- The Journal of clinical investigation, 2012
MicroRNA-122 (miR-122), which accounts for 70% of the liver's total miRNAs, plays a pivotal role in the liver. However, its intrinsic physiological roles remain largely undetermined. We demonstrated that mice lacking the gene encoding miR-122a (Mir122a) are viable but develop temporally controlled steatohepatitis, fibrosis, and hepatocellular carcinoma (HCC). These mice exhibited a striking disparity in HCC incidence based on sex, with a male-to-female ratio of 3.9:1, which recapitulates the disease incidence in humans. Impaired expression of microsomal triglyceride transfer protein (MTTP) contributed to steatosis, which was reversed by in vivo restoration of Mttp expression. We found that hepatic fibrosis onset can be partially attributed to the action of a miR-122a target, the Klf6 transcript. In addition, Mir122a(-/-) livers exhibited disruptions in a range of pathways, many of which closely resemble the disruptions found in human HCC. Importantly, the reexpression of miR-122a reduced disease manifestation and tumor incidence in Mir122a(-/-) mice. This study demonstrates that mice with a targeted deletion of the Mir122a gene possess several key phenotypes of human liver diseases, which provides a rationale for the development of a unique therapy for the treatment of chronic liver disease and HCC.
LinkOut: [PMID: 22820290]
86 mmu-miR-122-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003075 Tmem50b transmembrane protein 50B 1 1
MIRT003076 Lass6 ceramide synthase 6 1 1
MIRT003077 Gpx7 glutathione peroxidase 7 1 1
MIRT003113 Tgfbr1 transforming growth factor, beta receptor I 3 1
MIRT003114 Sbk1 SH3-binding kinase 1 3 1
MIRT003117 Hist1h1c histone cluster 1, H1c 3 1
MIRT003118 Ddc dopa decarboxylase 2 1
MIRT003120 Rell1 RELT-like 1 3 1
MIRT003121 Bach1 BTB and CNC homology 1, basic leucine zipper transcription factor 1 2 1
MIRT003122 Apob apolipoprotein B 2 1
MIRT003123 P4ha1 procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide 2 1
MIRT003124 Ccng1 cyclin G1 2 1
MIRT003125 Slc7a1 solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 3 3
MIRT003126 Gys1 glycogen synthase 1, muscle 2 2
MIRT003127 Slc35a4 solute carrier family 35, member A4 2 2
MIRT003128 Hfe2 hemochromatosis type 2 (juvenile) 3 3
MIRT003129 Tmed3 transmembrane p24 trafficking protein 3 2 2
MIRT003132 Bckdk branched chain ketoacid dehydrogenase kinase 4 1
MIRT003133 Aldoa aldolase A, fructose-bisphosphate 4 7
MIRT003134 Ndrg3 N-myc downstream regulated gene 3 4 2
MIRT003135 Cd320 CD320 antigen 4 1
MIRT003724 Smarcd1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 3 1
MIRT003725 Rcan1 regulator of calcineurin 1 2 1
MIRT003726 Gde1 glycerophosphodiester phosphodiesterase 1 2 1
MIRT004536 Ccrn4l nocturnin 4 1
MIRT005975 Klf6 Kruppel-like factor 6 4 1
MIRT005976 B2m beta-2 microglobulin 1 1
MIRT005977 Afp alpha fetoprotein 1 1
MIRT005978 Ccl2 chemokine (C-C motif) ligand 2 1 1
MIRT005979 Csf3r colony stimulating factor 3 receptor (granulocyte) 1 1
MIRT005980 Ctgf connective tissue growth factor 1 1
MIRT005981 Cxcl13 chemokine (C-X-C motif) ligand 13 1 1
MIRT005982 Cyp2b13 cytochrome P450, family 2, subfamily b, polypeptide 13 1 1
MIRT005983 Dbp D site albumin promoter binding protein 1 1
MIRT005984 Igf2 insulin-like growth factor 2 1 1
MIRT005985 Il1b interleukin 1 beta 1 1
MIRT005986 Jun jun proto-oncogene 1 1
MIRT005987 Per1 period circadian clock 1 1 1
MIRT005988 Alpl alkaline phosphatase, liver/bone/kidney 2 1
MIRT005989 Cs citrate synthase 2 1
MIRT005990 Prom1 prominin 1 3 1
MIRT005991 Sox4 SRY (sex determining region Y)-box 4 3 1
MIRT013527 Uros uroporphyrinogen III synthase 1 1
MIRT013528 Ppox protoporphyrinogen oxidase 1 1
MIRT013529 Urod uroporphyrinogen decarboxylase 1 1
MIRT013530 Cpox coproporphyrinogen oxidase 1 1
MIRT013531 Fech ferrochelatase 1 1
MIRT013532 Hamp hepcidin antimicrobial peptide 1 1
MIRT013533 Tfr2 transferrin receptor 2 1 1
MIRT013534 Smad4 SMAD family member 4 1 1
MIRT013535 Smad7 SMAD family member 7 1 1
MIRT013536 Bmpr1a bone morphogenetic protein receptor, type 1A 1 1
MIRT013537 Hba-a1 hemoglobin alpha, adult chain 1 2 1
MIRT013538 Hmbs hydroxymethylbilane synthase 2 1
MIRT013539 Mir17 microRNA 17 2 1
MIRT013540 Alas2 aminolevulinic acid synthase 2, erythroid 2 1
MIRT013541 Mir451 microRNA 451a 2 1
MIRT013542 Tfrc transferrin receptor 2 1
MIRT013543 Socs2 suppressor of cytokine signaling 2 1 1
MIRT013544 Slc35g1 solute carrier family 35, member G1 1 1
MIRT013545 Camk2b calcium/calmodulin-dependent protein kinase II, beta 1 1
MIRT013546 Irf6 interferon regulatory factor 6 1 1
MIRT013547 Rbl2 RB transcriptional corepressor like 2 1 1
MIRT013548 Hfe hemochromatosis 2 1
MIRT013549 Ccnd1 cyclin D1 2 1
MIRT054338 Pkm pyruvate kinase, muscle 3 1
MIRT054339 Tfdp2 transcription factor Dp 2 3 1
MIRT054340 E2f1 E2F transcription factor 1 3 1
MIRT579817 Zfp113 zinc finger protein 113 1 1
MIRT580378 Tmem206 transmembrane protein 206 1 2
MIRT581166 Sec23ip Sec23 interacting protein 1 1
MIRT582514 March5 membrane-associated ring finger (C3HC4) 5 1 2
MIRT586941 H2-T24 histocompatibility 2, T region locus 24 1 2
MIRT591859 Iffo2 intermediate filament family orphan 2 1 1
MIRT592160 Paxip1 PAX interacting (with transcription-activation domain) protein 1 1 1
MIRT592189 Nanog Nanog homeobox 1 2
MIRT592792 Cdh12 cadherin 12 1 1
MIRT597026 Thap1 THAP domain containing, apoptosis associated protein 1 1 1
MIRT597210 Snhg11 small nucleolar RNA host gene 11 1 1
MIRT601300 Zfp949 zinc finger protein 949 1 1
MIRT602608 Chrna1 cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) 1 1
MIRT602960 Xpo7 exportin 7 1 1
MIRT603391 Sgol2 shugoshin 2A 1 1
MIRT604008 Ggps1 geranylgeranyl diphosphate synthase 1 1 1
MIRT605553 Ppp1r16b protein phosphatase 1, regulatory (inhibitor) subunit 16B 1 1
MIRT606204 Sfxn4 sideroflexin 4 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-122 Quercetin NULL 5280343 Quantitative real-time PCR liver 22402395 2012 up-regulated
miR-122 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) NULL 15625 Quantitative real-time PCR thymus 23024791 2012 up-regulated
miR-122 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 up-regulated
miR-122 Streptozotocin (STZ) NULL 29327 Microarray streptozotocin (STZ)-induced type 1 diabetic mice 19372595 2009 down-regulated
miR-122 Streptozotocin (STZ) NULL 29327 Quantitative real-time PCR streptozotocin (STZ)-induced type 1 diabetic mice 19372595 2009 down-regulated
miR-122 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-122 Benzo(a)pyrene NULL 2336 Microarray Adult male B6C3F1 mice 21569818 2011 down-regulated
miR-122 Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated
miR-122 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-122 Bortezomib approved 387447 Quantitative real-time PCR SeAx and MyLa2000 cells 22235305 2012 up-regulated
miR-122 Doxorubicin approved 31703 Quantitative real-time PCR SeAx and MyLa2000 cells 22235305 2012 up-regulated
miR-122 MG132 NULL 462382 Quantitative real-time PCR SeAx and MyLa2000 cells 22235305 2012 up-regulated
miR-122 Microcystin-LR (MC-LR) NULL 445434 Quantitative real-time PCR WRL-68 Cells 22265967 2012 down-regulated
miR-122 Furan NULL 8029 Quantitative real-time PCR liver 22079235 2011 down-regulated
miR-122 Proanthocyanidins approved 108065 Quantitative real-time PCR liver cells 23922812 2013 down-regualted
miR-122 Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 down-regulated
miR-122 Dexamethasone approved 5743 Quantitative real-time PCR adrenals and granulosa cells 24205079 2014 down-regulated
miR-122 Proanthocyanidins approved 108065 Quantitative real-time PCR postprandial lipemia and liver 24445039 2014 down-regulated
miR-122 Aristolochic acid NULL 2236 Microarray kidney 25106556 2014 down-regualted
miR-122 Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 up-regulated
miR-122 Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-122-5p Cisplatin 5460033 NSC119875 approved sensitive Low Hepatocellular Carcinoma tissue and cell line
mmu-miR-122-5p Doxorubicin 31703 NSC123127 approved sensitive Low Hepatocellular Carcinoma tissue and cell line
mmu-miR-122-5p Fluorouracil 3385 NSC19893 approved sensitive Low Colon Cancer tissue and cell line (HCT-116, HCT-29)

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