miRTarBase - #MIRT006297


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pre-miRNA Information
pre-miRNA mmu-mir-320   
Genomic Coordinates chr14: 70443510 - 70443591
Synonyms Mirn320, mmu-mir-320, Mir320
Description Mus musculus miR-320 stem-loop
Comment Mouse mir-320 is predicted .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-320-3p
Sequence 48| AAAAGCUGGGUUGAGAGGGCGA |69
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B72VLT miR-320 Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Plasma MiRNA microarray; RT-PCR
B72VLT miR-320 Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Blood TaqMan OpenArray panels
B71YDE miR-320-3p Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Urine Real-time polymerase chain reaction
Gene Information
Gene Symbol Emilin2   
Synonyms FOAP-10
Description elastin microfibril interfacer 2
Transcript NM_145158   
Expression
Putative miRNA Targets on Emilin2
3'UTR of Emilin2
(miRNA target sites are highlighted)		 
>Emilin2|NM_145158|3'UTR
   1 GGAGACCAGGAAGGGAAGAAAGGCTGTGAAAACTCCAAAGCTTTAATATATTTGGTTTATGTAACTGGAGCACTGATCTA
  81 GCTTGCTGCACACACTCTGCCTGCCCCCAGGATAAAAGCCTGCCATCCCTCGTTTCCAGACAGCTGTGGAAGTGACACAT
 161 GGTTACTTCTGTCACTGGACAACCAGAAGACTCGTTATGTCCCCTTGGCTGGAAGCTGGCTTCTCCTGTGCCGCCTATAA
 241 GCCAGCTCTGCTCGAAGTTCTTGGGTTGAATCTACTTGGTGCTGTTCCGACTGGCCTGGGCCTGATGCAGCTGGAAGACA
 321 CTGTAGCGGATGCGCCTCTCTACACTGCACGTAGAAGATTTAAACACTGAAGTGCTTATGTGGCCTGGACCCAGAGAGTT
 401 TAACATGGGCTGTTGTTCCACACTTGGTTGCTGTTACCGTCTGGTACTGATCTCTGTTCCCCTGGGCCCTGCTACCAAGG
 481 TGGATGTGAGAGACATTATTTATGAAACTTGCCAGGCTCCTTAAATGACATGTACAATATAAGTGCAAAGACAGGGAGCG
 561 TCAATAAAGATGTTGGAATC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agCGGGAGA-GUUGGGUCGAAAa 5'
            |||  || :|| |||||| | 
Target 5' gtGCCGCCTATAAGCCAGCTCTg 3'
 228 - 250 132.00 -17.22
2 
miRNA  3' agcGGGAG---AGUUGGGUCGAAAa 5'
             |||||   || |  ||||| | 
Target 5' catCCCTCGTTTCCAGACAGCTGTg 3'
 124 - 148 127.00 -15.90
3 
miRNA  3' agcggGAGAGUUGGGUCGAAAa 5'
               ||| |||   |||||| 
Target 5' gaaaaCTC-CAA---AGCTTTa 3'
 28 - 45 124.00 -7.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions MMF , DB7 , COS-7
Disease 246707.0
Location of target site 3'UTR
Original Description (Extracted from the article) ... "Identi茂卢聛cation and characterization of Ets2 ...

- Bronisz A; Godlewski J; Wallace JA; et al., 2011, Nature cell biology.

Article - Bronisz A; Godlewski J; Wallace JA; et al.
- Nature cell biology, 2011
PTEN (Phosphatase and tensin homolog deleted on chromosome 10) expression in stromal fibroblasts suppresses epithelial mammary tumours, but the underlying molecular mechanisms remain unknown. Using proteomic and expression profiling, we show that Pten loss from mammary stromal fibroblasts activates an oncogenic secretome that orchestrates the transcriptional reprogramming of other cell types in the microenvironment. Downregulation of miR-320 and upregulation of one of its direct targets, ETS2 (v-ets erythroblastosis virus E26 oncogene homolog 2) are critical events in Pten-deleted stromal fibroblasts responsible for inducing this oncogenic secretome, which in turn promotes tumour angiogenesis and tumour-cell invasion. Expression of the Pten-miR-320-Ets2-regulated secretome distinguished human normal breast stroma from tumour stroma and robustly correlated with recurrence in breast cancer patients. This work reveals miR-320 as a critical component of the Pten tumour-suppressor axis that acts in stromal fibroblasts to reprogramme the tumour microenvironment and curtail tumour progression.
LinkOut: [PMID: 22179046]
27 mmu-miR-320-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004289 Hspb6 heat shock protein, alpha-crystallin-related, B6 3 1
MIRT006295 Ets2 E26 avian leukemia oncogene 2, 3' domain 2 1
MIRT006296 Mmp9 matrix metallopeptidase 9 2 1
MIRT006297 Emilin2 elastin microfibril interfacer 2 2 1
MIRT053611 Coro1a coronin, actin binding protein 1A 3 1
MIRT054739 E2f1 E2F transcription factor 1 3 1
MIRT054740 Sf1 splicing factor 1 3 1
MIRT579005 Cyp1a2 cytochrome P450, family 1, subfamily a, polypeptide 2 1 1
MIRT579685 Zzz3 zinc finger, ZZ domain containing 3 1 1
MIRT581633 Prickle2 prickle planar cell polarity protein 2 1 1
MIRT582038 P2ry1 purinergic receptor P2Y, G-protein coupled 1 1 1
MIRT582459 Mfsd4 major facilitator superfamily domain containing 4A 1 2
MIRT582850 Itga9 integrin alpha 9 1 1
MIRT583644 Tmem236 transmembrane protein 236 1 2
MIRT584207 Cpd carboxypeptidase D 1 1
MIRT587473 Synb syncytin b 1 2
MIRT587828 Ccdc121 coiled-coil domain containing 121 1 1
MIRT588538 Unc5c unc-5 netrin receptor C 1 1
MIRT588827 Spry4 sprouty homolog 4 (Drosophila) 1 1
MIRT594187 Igf2bp3 insulin-like growth factor 2 mRNA binding protein 3 1 1
MIRT594521 Bicd2 bicaudal D homolog 2 (Drosophila) 1 1
MIRT595316 Gns glucosamine (N-acetyl)-6-sulfatase 1 1
MIRT595371 Fam168b family with sequence similarity 168, member B 1 1
MIRT595533 Fosl2 fos-like antigen 2 1 1
MIRT596349 Serpine1 serine (or cysteine) peptidase inhibitor, clade E, member 1 1 1
MIRT606486 Cplx2 complexin 2 1 1
MIRT737047 Cd36 CD36 antigen 6 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-320 Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 up-regulated
miR-320 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 up-regulated
miR-320 5-aza-2'-deoxycytidine (5-Aza-CdR) approved 451668 Microarray Pancreatic Cancer MiaPACA-2 cells 19407485 2009 up-regulated
miR-320 5-aza-2'-deoxycytidine (5-Aza-CdR) approved 451668 Microarray Pancreatic Cancer PANC-1 cells 19407485 2009 up-regulated
miR-320 5-aza-2'-deoxycytidine (5-Aza-CdR) + trichostatin A(TSA) NULL NULL Microarray Pancreatic Cancer MiaPACA-2 cells 19407485 2009 up-regulated
miR-320 5-aza-2'-deoxycytidine (5-Aza-CdR) + trichostatin A(TSA) NULL NULL Microarray Pancreatic Cancer PANC-1 cells 19407485 2009 up-regulated
miR-320 Trichostatin A (TSA) NULL 444732 Microarray Pancreatic Cancer MiaPACA-2 cells 19407485 2009 up-regulated
miR-320 Trichostatin A (TSA) NULL 444732 Microarray Pancreatic Cancer PANC-1 cells 19407485 2009 up-regulated
miR-320 Diethylstilbestrol approved 448537 Microarray mammosphere-derived epithelial cells (MDEC) 19549897 2009 down-regulated
miR-320 Diethylstilbestrol approved 448537 Quantitative real-time PCR mammosphere-derived epithelial cells (MDEC) 19549897 2009 down-regulated
miR-320 Bio-Oss NULL NULL Microarray osteoblast-like cell line (MG63) 20224834 2010 up-regulated
miR-320 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells HT-29 cells 20955366 2011 down-regulated
miR-320 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 down-regulated
miR-320 Budesonide approved 5281004 Microarray neonatal mice liver 20145010 2010 down-regulated
miR-320 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 up-regulated
miR-320 Isoproterenol approved 3779 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-320 Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 up-regulated
miR-320 Aristolochic acid NULL 2236 Microarray kidney 25106556 2014 down-regualted
miR-320 Hydroxamic acid HDACi LAQ824 NULL NULL Northern blot breast cancer cell line SKBr3 16452180 2006 up-regulated

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