miRTarBase - #MIRT006310


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pre-miRNA Information
pre-miRNA mmu-mir-125a   
Genomic Coordinates chr17: 17830812 - 17830879
Description Mus musculus miR-125a stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-125a-5p
Sequence 6| UCCCUGAGACCCUUUAACCUGUGA |29
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Cbx7   
Synonyms 1600014J01Rik, AI851678, D15Ertd417e
Description chromobox 7
Transcript NM_144811   
Expression
Putative miRNA Targets on Cbx7
3'UTR of Cbx7
(miRNA target sites are highlighted)		 
>Cbx7|NM_144811|3'UTR
   1 ATAGCGGCTTCCCCTCTTAAAACTGTGCTTCTTGGGGCTTGGAGGCTGATCCGTGGAGCCGGAGGAGCCCAGGAGGTCCA
  81 GACCTTGCCTCAGGCAGTCACCCCAGCTTTCTGGTGGTGTCCCTTCCCTGTCCCTTGCCGTGGGTCTTTACTATGAACTT
 161 TCCACTCCAGCCTTGTGGTGGGACACAGAGGCCGGCCTTGGCCACCGGGGACAGGATCAGGACAACCGGCACGGGCTGGA
 241 CCAGGGAAGAGGCTGCCCACCAAGCCTCCTCCGAGTGTGCTGTCTCCCTGCCATTGGCCTGTGTAGAGATGCCCATGAAC
 321 ACATCCGGACACCCCAACATGCACACCTTTAGAAAACTGACCAGTTGAACATGACACCATTCACAGCACAAACAGAACTT
 401 GCCGCGGCACCCTTGCTCCGCCTTGCACGTAGGTCATGTGACAGGATCTGTCGGCTGTCATCTCCGAGCCGCCAATTGGA
 481 CCAGGCTTTGGTGTGCAAGGTGCAGATGCTTCCACACAGGCTCACAGCCACCTGGCCCACCCTTCCTCCCACAGGAGTTC
 561 ACATTCTTTCTGACTCAGGTGACTTTCCAGCCCCGCCCCCCAATTTCTCTGCCCTCTCCTGCCAATCAGCCGATCTGGGC
 641 ACCCATGGGTGCCAGTGGTATGTGGGCAGTCAGGGAGGGAGTGTCCCACCACTTTCTCAGGGCAGTCCTTGTCTTCTTTG
 721 TCCCCCTCCCCACCCAACCTGAATGGCATGGCTAAGGATGGATGACCCCTCCCACCTTGTGGGGGTTGAACATGAATCAG
 801 GTTGCACACAAGTGTGTGGTCCTGATTTGGCAAGGGGGGGGGTGCGGTTTGCTGGTTTGCACAGGTGCCCTATAGGCTGA
 881 CAGCTTCCTACCTGAGGACGGCTCCCTCCCTTGTCGAGAGCACATTGCTCACACACCCGCTGCTACCATACGAACCTATA
 961 TGGCTGGGTCCTAGCAGCTACCAATTCTGCTGTGTCTTTAGGAGGCCAGCAGAGTCAGTATGAACAAGGGCAGGATGACT
1041 TCTAAACACTACCCTGCCCCGTGTCTGTCTGCGACTCTATCCTCTTCCTTCTCTAGTCCCTGGTCTCCCCCTCCTTTAGT
1121 CTTAATGGTCCAGGGTTAACTCAGTGCTTCCGTTGGTGGCCGTCATCTGTGGGTGACCTGAATCTAGAGGATGTCTGGGT
1201 GGGCCAGAGAGAGGAGAGACAGGAACCTCAGCCAGTAGGCTACAGGAACCTGGAGGTGGGGAGACCTTGGTGTTCTTGAA
1281 GCCCCAGCTCCCAGAGAGGTGAGGGTGGCTGGAGCTTTTCTTTGCTGCTGGGTTTGATGGCCCACCATATTGGGGGACAG
1361 AATCCCTTCGGTGTCATGCTTCCCATCCCTAGGGGCATGGGACTAAATGCCTGTACAATTTGAATTTTGATCATGAGACC
1441 CCAGGGGGGGCTTTCCAAGCAGCTGCCTTCTGTCTTTTGCAGTAGGCGGTGACCTTTTATCTCACGGGGCCCTTTATGAA
1521 ACCCACCAGTCCCTGGAGATTGTGGACTAGGCTGGATATCCCTCAGGGTAGCCATGTAGCTCTGAACTGCTGGAAACTGG
1601 CCGCCTAGGGAGAATGGCAGAAGGCTTCGAGTGGCTATACACAGGCTTCTCCTAGCCTGAAGCTACCCAAGTGGACAGTA
1681 ACACGCCCCCTCTCCACCCACATACACTAACCCCATACAGGGCCAGTACTCCATGAGCCAGCAGTGGCGACCACCTGCCT
1761 ATCTCTTAACCCCTCCCCCTGCATATTTGAATGTAGAGGTTGGGTTGAACTTCCCCCTCCCTGGGTTGGGGTGAAGATGT
1841 GCCTCAACCTCTGGTGTGGACACTTGTGCAGTGCCACAGTTGAGCCCATTTGGCCCACGTGGTTCTCATCTGGTAAGATG
1921 GTGATCTTATTGCCAAGTTCCTTCCACCTCCTGCCTACCGAGGAAGTAGCTCGGTGTCAGCGAGGTGGTATCGATTTTGC
2001 TTTATAAATGGCGGGCCAGACCTGGCCACCAGGTCTTTGCGCAAGACCGCTTCCTTTGCACACAGAATGAGCTACGAGCT
2081 TTGTTCAGACGACCTGAATGTATTGGGAGGAATTGGGCATAGGCCTTCCCCCGGGGGGAGTGCGATGGGAAGAGTAAGCA
2161 GGTGTAGGCTGCAGTTTTATTTTTGTACTGATATTGGTAAAAGACTGTATAGCATCTATTTATTTAGATGATTTATCTGA
2241 TAGATGAGGCAAAAATTATTAAAAAATACATTAAAATGTCTTTAGA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agUG-UCCAAUUUCCCA--GAGUCCCu 5'
            || ||| |::|   |  ||||||| 
Target 5' ggACTAGGCTGGA-TATCCCTCAGGGt 3'
 1544 - 1569 147.00 -16.00
2 
miRNA  3' aguguccaauuucccAGAGUCCCu 5'
                         |||||||| 
Target 5' agtgtcccaccacttTCTCAGGGc 3'
 680 - 703 145.00 -14.40
3 
miRNA  3' agugUCCA-AUUU--CCCAGAGUCCcu 5'
              ||||  |:|    | ||||||  
Target 5' caggAGGTCCAGACCTTGCCTCAGGca 3'
 70 - 96 126.00 -15.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions IMR90 , CCE , E14 , ZHBTc4 , LF2 , PGK12.1 , 46C , Oct4-GIP , B05 , F05
Location of target site 3'UTR
Original Description (Extracted from the article) ... These results demonstrate that miR-125b and miR-181a/b are induced during ESC differentiation and contribute to this process by downregulating Cbx7 expression through direct targeting of its3'UTR. ...

- O'Loghlen A; Munoz-Cabello AM; Gaspar-Maia et al., 2012, Cell stem cell.

Article - O'Loghlen A; Munoz-Cabello AM; Gaspar-Maia et al.
- Cell stem cell, 2012
The Polycomb Group (PcG) of chromatin modifiers regulates pluripotency and differentiation. Mammalian genomes encode multiple homologs of the Polycomb repressive complex 1 (PRC1) components, including five orthologs of the Drosophila Polycomb protein (Cbx2, Cbx4, Cbx6, Cbx7, and Cbx8). We have identified Cbx7 as the primary Polycomb ortholog of PRC1 complexes in embryonic stem cells (ESCs). The expression of Cbx7 is downregulated during ESC differentiation, preceding the upregulation of Cbx2, Cbx4, and Cbx8, which are directly repressed by Cbx7. Ectopic expression of Cbx7 inhibits differentiation and X chromosome inactivation and enhances ESC self-renewal. Conversely, Cbx7 knockdown induces differentiation and derepresses lineage-specific markers. In a functional screen, we identified the miR-125 and miR-181 families as regulators of Cbx7 that are induced during ESC differentiation. Ectopic expression of these miRNAs accelerates ESC differentiation via regulation of Cbx7. These observations establish a critical role for Cbx7 and its regulatory miRNAs in determining pluripotency.
LinkOut: [PMID: 22226354]
28 mmu-miR-125a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001716 Lin28a lin-28 homolog A (C. elegans) 1 1
MIRT005597 Trim71 tripartite motif-containing 71 4 3
MIRT006310 Cbx7 chromobox 7 2 1
MIRT006967 4632428N05Rik V-set immunoregulatory receptor 2 1
MIRT054177 Ptpn18 protein tyrosine phosphatase, non-receptor type 18 2 1
MIRT054178 Ptpn7 protein tyrosine phosphatase, non-receptor type 7 2 1
MIRT054179 Ppp1ca protein phosphatase 1, catalytic subunit, alpha isoform 2 1
MIRT054180 Ppp2ca protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform 2 1
MIRT054570 Traf6 TNF receptor-associated factor 6 3 1
MIRT580227 Tspan12 tetraspanin 12 2 2
MIRT582368 Mtf1 metal response element binding transcription factor 1 2 2
MIRT582450 Mgat4a mannoside acetylglucosaminyltransferase 4, isoenzyme A 2 2
MIRT584884 Antxr2 anthrax toxin receptor 2 2 2
MIRT587193 Foxk1 forkhead box K1 2 2
MIRT588848 Snrnp40 small nuclear ribonucleoprotein 40 (U5) 2 2
MIRT591275 Lars2 leucyl-tRNA synthetase, mitochondrial 2 4
MIRT592914 Madd MAP-kinase activating death domain 2 2
MIRT594893 Il1rn interleukin 1 receptor antagonist 2 4
MIRT596427 Hif1an hypoxia-inducible factor 1, alpha subunit inhibitor 3 3
MIRT599142 Dqx1 DEAQ RNA-dependent ATPase 2 2
MIRT599717 Aph1c aph1 homolog C, gamma secretase subunit 2 2
MIRT600832 Gm14137 predicted gene 14137 2 2
MIRT604902 Jmy junction-mediating and regulatory protein 2 2
MIRT605441 Stat1 signal transducer and activator of transcription 1 2 2
MIRT606092 Wdr25 WD repeat domain 25 2 2
MIRT732537 Tnfrsf1b tumor necrosis factor receptor superfamily, member 1b 4 0
MIRT734700 VDR vitamin D receptor 2 0
MIRT734824 Sox11 SRY (sex determining region Y)-box 11 2 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-125a Budesonide approved 5281004 Microarray neonatal mice liver 20145010 2010 down-regulated
miR-125a Phenethyl isothiocyanate(PEITC) NULL 16741 Microarray neonatal mice lung 20145010 2010 down-regulated
miR-125a Chaihu Shugan San NULL NULL Microarray hippocampus 23947143 2013 down-regualted
miR-125a Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated
miR-125a Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 up-regulated
miR-125a Enoxacin approved 3229 Quantitative real-time PCR HEK293 cells 18641635 2008 up-regulated
miR-125a 4-hydroxynonenal NULL 5283344 Microarray human leukemic HL-60 cell 19022373 2009 up-regulated
miR-125a Valproate approved 3121 Quantitative real-time PCR CD4+, CD25- T cells 20427269 2010 down-regulated
miR-125a Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-125a Enoxacin approved 3229 Northern blot HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-125a Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-125a 5-aza-2'-deoxycytidine (5-Aza-CdR) approved 451668 Microarray breast cancer MDA-MB231 22076154 2011 down-regulated
miR-125a-5p Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 up-regulated
miR-125a-5p Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 down-regulated
miR-125a-5p Curcumin NULL 969516 Microarray HONE1 cells 24896104 2014 down-regulated
miR-125a-5p 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-125a-5p Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-125a-5p Trastuzumab approved NULL Microarray SKBR3 cells. 22384020 2012 up-regulated
miR-125a-5p Glucocorticoid NULL NULL TaqMan low-density array Eosinophilic esophagitis 22815788 2012 up-regulated
miR-125a-5p Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-125a-5p Paclitaxel 36314 NSC125973 approved sensitive Low Colon Cancer tissue and cell line (HT-29)

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