miRTarBase - #MIRT006312


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pre-miRNA Information
pre-miRNA mmu-mir-181b-1   
Genomic Coordinates chr1: 137966639 - 137966718
Description Mus musculus miR-181b-1 stem-loop
Comment Mouse miR-181b was predicted by computational methods using conservation with human and Fugu rubripes sequences .
RNA Secondary Structure
pre-miRNA mmu-mir-181b-2   
Genomic Coordinates chr2: 38853830 - 38853918
Synonyms Mirn181b-2, mir-181b-2, Mir181b-2
Description Mus musculus miR-181b-2 stem-loop
Comment Mouse miR-181b was predicted by computational methods using conservation with human and Fugu rubripes sequences .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-181b-5p
Sequence 12| AACAUUCAUUGCUGUCGGUGGGU |34
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
BK9AD8 miR-181b-5p Monitoring Biomarker (MOI) Clinical/Experimental Data Expression Decrease Peripheral blood Quantitative reverse transcription PCR
Gene Information
Gene Symbol Cbx7   
Synonyms 1600014J01Rik, AI851678, D15Ertd417e
Description chromobox 7
Transcript NM_144811   
Expression
Putative miRNA Targets on Cbx7
3'UTR of Cbx7
(miRNA target sites are highlighted)		 
>Cbx7|NM_144811|3'UTR
   1 ATAGCGGCTTCCCCTCTTAAAACTGTGCTTCTTGGGGCTTGGAGGCTGATCCGTGGAGCCGGAGGAGCCCAGGAGGTCCA
  81 GACCTTGCCTCAGGCAGTCACCCCAGCTTTCTGGTGGTGTCCCTTCCCTGTCCCTTGCCGTGGGTCTTTACTATGAACTT
 161 TCCACTCCAGCCTTGTGGTGGGACACAGAGGCCGGCCTTGGCCACCGGGGACAGGATCAGGACAACCGGCACGGGCTGGA
 241 CCAGGGAAGAGGCTGCCCACCAAGCCTCCTCCGAGTGTGCTGTCTCCCTGCCATTGGCCTGTGTAGAGATGCCCATGAAC
 321 ACATCCGGACACCCCAACATGCACACCTTTAGAAAACTGACCAGTTGAACATGACACCATTCACAGCACAAACAGAACTT
 401 GCCGCGGCACCCTTGCTCCGCCTTGCACGTAGGTCATGTGACAGGATCTGTCGGCTGTCATCTCCGAGCCGCCAATTGGA
 481 CCAGGCTTTGGTGTGCAAGGTGCAGATGCTTCCACACAGGCTCACAGCCACCTGGCCCACCCTTCCTCCCACAGGAGTTC
 561 ACATTCTTTCTGACTCAGGTGACTTTCCAGCCCCGCCCCCCAATTTCTCTGCCCTCTCCTGCCAATCAGCCGATCTGGGC
 641 ACCCATGGGTGCCAGTGGTATGTGGGCAGTCAGGGAGGGAGTGTCCCACCACTTTCTCAGGGCAGTCCTTGTCTTCTTTG
 721 TCCCCCTCCCCACCCAACCTGAATGGCATGGCTAAGGATGGATGACCCCTCCCACCTTGTGGGGGTTGAACATGAATCAG
 801 GTTGCACACAAGTGTGTGGTCCTGATTTGGCAAGGGGGGGGGTGCGGTTTGCTGGTTTGCACAGGTGCCCTATAGGCTGA
 881 CAGCTTCCTACCTGAGGACGGCTCCCTCCCTTGTCGAGAGCACATTGCTCACACACCCGCTGCTACCATACGAACCTATA
 961 TGGCTGGGTCCTAGCAGCTACCAATTCTGCTGTGTCTTTAGGAGGCCAGCAGAGTCAGTATGAACAAGGGCAGGATGACT
1041 TCTAAACACTACCCTGCCCCGTGTCTGTCTGCGACTCTATCCTCTTCCTTCTCTAGTCCCTGGTCTCCCCCTCCTTTAGT
1121 CTTAATGGTCCAGGGTTAACTCAGTGCTTCCGTTGGTGGCCGTCATCTGTGGGTGACCTGAATCTAGAGGATGTCTGGGT
1201 GGGCCAGAGAGAGGAGAGACAGGAACCTCAGCCAGTAGGCTACAGGAACCTGGAGGTGGGGAGACCTTGGTGTTCTTGAA
1281 GCCCCAGCTCCCAGAGAGGTGAGGGTGGCTGGAGCTTTTCTTTGCTGCTGGGTTTGATGGCCCACCATATTGGGGGACAG
1361 AATCCCTTCGGTGTCATGCTTCCCATCCCTAGGGGCATGGGACTAAATGCCTGTACAATTTGAATTTTGATCATGAGACC
1441 CCAGGGGGGGCTTTCCAAGCAGCTGCCTTCTGTCTTTTGCAGTAGGCGGTGACCTTTTATCTCACGGGGCCCTTTATGAA
1521 ACCCACCAGTCCCTGGAGATTGTGGACTAGGCTGGATATCCCTCAGGGTAGCCATGTAGCTCTGAACTGCTGGAAACTGG
1601 CCGCCTAGGGAGAATGGCAGAAGGCTTCGAGTGGCTATACACAGGCTTCTCCTAGCCTGAAGCTACCCAAGTGGACAGTA
1681 ACACGCCCCCTCTCCACCCACATACACTAACCCCATACAGGGCCAGTACTCCATGAGCCAGCAGTGGCGACCACCTGCCT
1761 ATCTCTTAACCCCTCCCCCTGCATATTTGAATGTAGAGGTTGGGTTGAACTTCCCCCTCCCTGGGTTGGGGTGAAGATGT
1841 GCCTCAACCTCTGGTGTGGACACTTGTGCAGTGCCACAGTTGAGCCCATTTGGCCCACGTGGTTCTCATCTGGTAAGATG
1921 GTGATCTTATTGCCAAGTTCCTTCCACCTCCTGCCTACCGAGGAAGTAGCTCGGTGTCAGCGAGGTGGTATCGATTTTGC
2001 TTTATAAATGGCGGGCCAGACCTGGCCACCAGGTCTTTGCGCAAGACCGCTTCCTTTGCACACAGAATGAGCTACGAGCT
2081 TTGTTCAGACGACCTGAATGTATTGGGAGGAATTGGGCATAGGCCTTCCCCCGGGGGGAGTGCGATGGGAAGAGTAAGCA
2161 GGTGTAGGCTGCAGTTTTATTTTTGTACTGATATTGGTAAAAGACTGTATAGCATCTATTTATTTAGATGATTTATCTGA
2241 TAGATGAGGCAAAAATTATTAAAAAATACATTAAAATGTCTTTAGA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugGGUGGCUGUCGU-U--ACUUACAa 5'
            || ||  | ||| |  ||||||| 
Target 5' ccCCTCCCCCTGCATATTTGAATGTa 3'
 1770 - 1795 155.00 -16.50
2 
miRNA  3' uggguGGCUGUCGUUACUUACAa 5'
               | |||: |  ||||||| 
Target 5' ttgttCAGACGAC-CTGAATGTa 3'
 2081 - 2102 149.00 -11.20
3 
miRNA  3' ugGGUGGCUGUCGUUACUUACaa 5'
            |||||  || |  ||||||  
Target 5' ccCCACC--CAAC-CTGAATGgc 3'
 728 - 747 136.00 -13.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CCE , E14 , ZHBTc4 , LF2 , PGK12.1 , 46C , Oct4-GIP , B05 , F05
Location of target site 3'UTR
Original Description (Extracted from the article) ... These results demonstrate that miR-125b and miR-181a/b are induced during ESC differentiation and contribute to this process by downregulating Cbx7 expression through direct targeting of its 3'UTR. ...

- O'Loghlen A; Munoz-Cabello AM; Gaspar-Maia et al., 2012, Cell stem cell.

Article - O'Loghlen A; Munoz-Cabello AM; Gaspar-Maia et al.
- Cell stem cell, 2012
The Polycomb Group (PcG) of chromatin modifiers regulates pluripotency and differentiation. Mammalian genomes encode multiple homologs of the Polycomb repressive complex 1 (PRC1) components, including five orthologs of the Drosophila Polycomb protein (Cbx2, Cbx4, Cbx6, Cbx7, and Cbx8). We have identified Cbx7 as the primary Polycomb ortholog of PRC1 complexes in embryonic stem cells (ESCs). The expression of Cbx7 is downregulated during ESC differentiation, preceding the upregulation of Cbx2, Cbx4, and Cbx8, which are directly repressed by Cbx7. Ectopic expression of Cbx7 inhibits differentiation and X chromosome inactivation and enhances ESC self-renewal. Conversely, Cbx7 knockdown induces differentiation and derepresses lineage-specific markers. In a functional screen, we identified the miR-125 and miR-181 families as regulators of Cbx7 that are induced during ESC differentiation. Ectopic expression of these miRNAs accelerates ESC differentiation via regulation of Cbx7. These observations establish a critical role for Cbx7 and its regulatory miRNAs in determining pluripotency.
LinkOut: [PMID: 22226354]
56 mmu-miR-181b-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004755 Timp3 tissue inhibitor of metalloproteinase 3 4 1
MIRT005296 Aicda activation-induced cytidine deaminase 5 1
MIRT005300 Slc35d3 solute carrier family 35, member D3 2 1
MIRT005301 Ak4 adenylate kinase 4 2 1
MIRT005302 Ndrg1 N-myc downstream regulated gene 1 2 1
MIRT005303 Pitpnc1 phosphatidylinositol transfer protein, cytoplasmic 1 2 1
MIRT005304 Tmem14a transmembrane protein 14A 2 1
MIRT005305 Cd244 CD244 natural killer cell receptor 2B4 2 1
MIRT005306 Gch1 GTP cyclohydrolase 1 2 1
MIRT005307 Errfi1 ERBB receptor feedback inhibitor 1 2 1
MIRT005308 Hk2 hexokinase 2 2 1
MIRT005309 Mxi1 MAX interactor 1, dimerization protein 2 1
MIRT005310 Ccdc58 coiled-coil domain containing 58 2 1
MIRT005311 Sc4mol methylsterol monoxygenase 1 2 1
MIRT005312 Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 2 1
MIRT005313 Pdxp pyridoxal (pyridoxine, vitamin B6) phosphatase 2 1
MIRT005314 Atp5g1 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9) 2 1
MIRT005315 Casp3 caspase 3 2 1
MIRT005316 Ulk3 unc-51-like kinase 3 2 1
MIRT005317 Snx25 sorting nexin 25 2 1
MIRT005318 Higd1a HIG1 domain family, member 1A 2 1
MIRT006312 Cbx7 chromobox 7 2 1
MIRT438370 SIX2 SIX homeobox 2 3 1
MIRT438687 Hspa5 heat shock protein 5 3 1
MIRT438688 Uchl1 ubiquitin carboxy-terminal hydrolase L1 3 1
MIRT577184 Smarca4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 1 5
MIRT577425 Tulp3 tubby-like protein 3 1 1
MIRT578325 Magea5 melanoma antigen, family A, 5 1 1
MIRT579346 Arhgef15 Rho guanine nucleotide exchange factor (GEF) 15 1 4
MIRT580671 Stk32a serine/threonine kinase 32A 1 1
MIRT580762 Spp1 secreted phosphoprotein 1 1 1
MIRT581071 Sgpp1 sphingosine-1-phosphate phosphatase 1 1 1
MIRT582047 Osbpl3 oxysterol binding protein-like 3 1 1
MIRT582656 Lnp lunapark, ER junction formation factor 1 2
MIRT583661 Fam160b2 family with sequence similarity 160, member B2 1 5
MIRT583691 Fam122b family with sequence similarity 122, member B 1 1
MIRT584580 Bnc2 basonuclin 2 1 1
MIRT584878 Ap1g1 adaptor protein complex AP-1, gamma 1 subunit 1 1
MIRT586224 Ptchd2 dispatched RND transporter family member 3 1 2
MIRT587223 Fam69c family with sequence similarity 69, member C 1 1
MIRT588616 Trim2 tripartite motif-containing 2 1 1
MIRT589131 Rad51d RAD51 paralog D 1 1
MIRT589794 Ikzf2 IKAROS family zinc finger 2 1 2
MIRT590593 Atp2a2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 1 1
MIRT591186 Ms4a4c membrane-spanning 4-domains, subfamily A, member 4C 1 3
MIRT591489 Aqp3 aquaporin 3 1 2
MIRT592858 Adamts9 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9 1 2
MIRT594856 Nacc1 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing 1 1
MIRT594983 Ebf3 early B cell factor 3 1 1
MIRT596592 Zfp689 zinc finger protein 689 1 1
MIRT598537 Itpk1 inositol 1,3,4-triphosphate 5/6 kinase 1 1
MIRT598728 H2-D1 histocompatibility 2, D region locus 1 1 1
MIRT600002 4930563E22Rik RIKEN cDNA 4930563E22 gene 1 1
MIRT603966 Gpd1l glycerol-3-phosphate dehydrogenase 1-like 1 1
MIRT605134 Clmn calmin 1 1
MIRT606009 Dagla diacylglycerol lipase, alpha 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-181b Teniposide approved 452548 Quantitative real-time PCR glioma cells 25151861 2014 up-regualted
miR-181b All-trans-retinoic acid (ATRA) approved 444795 Microarray acute promyelocytic leukemia 17260024 2008 down-regulated
miR-181b Curcumin NULL 969516 Microarray BxPC-3 human pancreatic carcinoma cell line 18347134 2008 up-regulated
miR-181b Trichostatin A (TSA) NULL 444732 Microarray human pancreatic cancer cell line BxPC-3 19112422 2009 down-regulated
miR-181b Doxorubicin approved 31703 Microarray ovarian cancer 19237188 2009 up-regulated
miR-181b Gemcitabine approved 60750 Microarray ovarian cancer 19237188 2009 up-regulated
miR-181b All-trans-retinoic acid (ATRA) approved 444795 Quantitative real-time PCR acute promyelocytic leukemia (APL) 19749800 2009 down-regulated
miR-181b Morphine approved 5288826 Microarray human monocyte-derived macrophages (h-mdms) infection with HIV-1 20564181 2010 down-regulated
miR-181b Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-181b Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-181b Ginsenoside Rh2 NULL 119307 Microarray human glioma cells U251 21372826 2011 up-regulated
miR-181b Aidi injection NULL NULL Microarray human breast cancer cells 21563499 2011 down-regulated
miR-181b Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-181b Olea europaea leaf extract NULL NULL Quantitative real-time PCR glioblastoma cells. 22722712 2012 up-regulated
miR-181b Temozolomide approved 5394 Quantitative real-time PCR glioblastoma cells. 22722712 2012 up-regulated
miR-181b Celecoxib approved 2662 Microarray gastric cancer cells. 23001726 2013 up-regulated
miR-181b 17beta-estradiol (E2) approved 5757 Microarray rat breast 17700064 2007 up-regulated
miR-181b Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-181b Estrogen NULL NULL Microarray mouse uterus 20720260 2010 down-regulated
miR-181b Estrogen NULL NULL Quantitative real-time PCR mouse uterus 20720260 2010 down-regulated
miR-181b Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 down-regulated
miR-181b Nicotine approved 89594 Microarray Rat adrenal pheochromocytoma PC12 cell 18845019 2009 up-regulated
miR-181b Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated
miR-181b Perfluorooctane sulfonate NULL 74483 Quantitative real-time PCR zebrafish embryos 20878907 2011 down-regulated
miR-181b Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-181b-5p Gemcitabine 60750 NSC613327 approved sensitive Low Pancreatic Ductal Adenocarcinoma tissue and cell line (SW1990, CFPAC-1)

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