miRTarBase - #MIRT006349


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pre-miRNA Information
pre-miRNA hsa-mir-449b   
Genomic Coordinates chr5: 55170646 - 55170742
Description Homo sapiens miR-449b stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-449b-5p
Sequence 16| AGGCAGUGUAUUGUUAGCUGGC |37
Evidence Experimental
Experiments Microarray
DRVs in miRNA
Mutant ID Mutant Position Mutant Source
COSN8871076 12 COSMIC
COSN17153496 22 COSMIC
COSN31576772 22 COSMIC
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1322925717 3 dbSNP
rs769526811 10 dbSNP
rs10061133 12 dbSNP
rs1431829587 13 dbSNP
rs778042202 21 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol SIRT1   
Synonyms SIR2, SIR2L1, SIR2alpha
Description sirtuin 1
Transcript NM_001142498   
Other Transcripts NM_012238   
Expression
Putative miRNA Targets on SIRT1
3'UTR of SIRT1
(miRNA target sites are highlighted)		 
>SIRT1|NM_001142498|3'UTR
   1 TGTAATAATTGTGCAGGTACAGGAATTGTTCCACCAGCATTAGGAACTTTAGCATGTCAAAATGAATGTTTACTTGTGAA
  81 CTCGATAGAGCAAGGAAACCAGAAAGGTGTAATATTTATAGGTTGGTAAAATAGATTGTTTTTCATGGATAATTTTTAAC
 161 TTCATTATTTCTGTACTTGTACAAACTCAACACTAACTTTTTTTTTTTTAAAAAAAAAAAGGTACTAAGTATCTTCAATC
 241 AGCTGTTGGTCAAGACTAACTTTCTTTTAAAGGTTCATTTGTATGATAAATTCATATGTGTATATATAATTTTTTTTGTT
 321 TTGTCTAGTGAGTTTCAACATTTTTAAAGTTTTCAAAAAGCCATCGGAATGTTAAATTAATGTAAAGGGAACAGCTAATC
 401 TAGACCAAAGAATGGTATTTTCACTTTTCTTTGTAACATTGAATGGTTTGAAGTACTCAAAATCTGTTACGCTAAACTTT
 481 TGATTCTTTAACACAATTATTTTTAAACACTGGCATTTTCCAAAACTGTGGCAGCTAACTTTTTAAAATCTCAAATGACA
 561 TGCAGTGTGAGTAGAAGGAAGTCAACAATATGTGGGGAGAGCACTCGGTTGTCTTTACTTTTAAAAGTAATACTTGGTGC
 641 TAAGAATTTCAGGATTATTGTATTTACGTTCAAATGAAGATGGCTTTTGTACTTCCTGTGGACATGTAGTAATGTCTATA
 721 TTGGCTCATAAAACTAACCTGAAAAACAAATAAATGCTTTGGAAATGTTTCAGTTGCTTTAGAAACATTAGTGCCTGCCT
 801 GGATCCCCTTAGTTTTGAAATATTTGCCATTGTTGTTTAAATACCTATCACTGTGGTAGAGCTTGCATTGATCTTTTCCA
 881 CAAGTATTAAACTGCCAAAATGTGAATATGCAAAGCCTTTCTGAATCTATAATAATGGTACTTCTACTGGGGAGAGTGTA
 961 ATATTTTGGACTGCTGTTTTCCATTAATGAGGAGAGCAACAGGCCCCTGATTATACAGTTCCAAAGTAATAAGATGTTAA
1041 TTGTAATTCAGCCAGAAAGTACATGTCTCCCATTGGGAGGATTTGGTGTTAAATACCAAACTGCTAGCCCTAGTATTATG
1121 GAGATGAACATGATGATGTAACTTGTAATAGCAGAATAGTTAATGAATGAAACTAGTTCTTATAATTTATCTTTATTTAA
1201 AAGCTTAGCCTGCCTTAAAACTAGAGATCAACTTTCTCAGCTGCAAAAGCTTCTAGTCTTTCAAGAAGTTCATACTTTAT
1281 GAAATTGCACAGTAAGCATTTATTTTTCAGACCATTTTTGAACATCACTCCTAAATTAATAAAGTATTCCTCTGTTGCTT
1361 TAGTATTTATTACAATAAAAAGGGTTTGAAATATAGCTGTTCTTTATGCATAAAACACCCAGCTAGGACCATTACTGCCA
1441 GAGAAAAAAATCGTATTGAATGGCCATTTCCCTACTTATAAGATGTCTCAATCTGAATTTATTTGGCTACACTAAAGAAT
1521 GCAGTATATTTAGTTTTCCATTTGCATGATGTTTGTGTGCTATAGATGATATTTTAAATTGAAAAGTTTGTTTTAAATTA
1601 TTTTTACAGTGAAGACTGTTTTCAGCTCTTTTTATATTGTACATAGTCTTTTATGTAATTTACTGGCATATGTTTTGTAG
1681 ACTGTTTAATGACTGGATATCTTCCTTCAACTTTTGAAATACAAAACCAGTGTTTTTTACTTGTACACTGTTTTAAAGTC
1761 TATTAAAATTGTCATTTGACTTTTTTCTGTTAACTTAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cgGUCGAU--UGUUAUGUGACGGa 5'
            ||||||  || || :|||||| 
Target 5' ccCAGCTAGGACCAT-TACTGCCa 3'
 1418 - 1440 153.00 -21.70
2 
miRNA  3' cgGUCGAUUGUUAUGUGACGGa 5'
            || | |::| || |||||| 
Target 5' tcCA-CAAGTATTAAACTGCCa 3'
 877 - 897 131.00 -11.90
3 
miRNA  3' cggucGAUUGUUAUGUGACGGa 5'
               :|| : :|||||||:: 
Target 5' gttttTTACTTGTACACTGTTt 3'
 1732 - 1753 125.00 -11.70
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN6980624 4 COSMIC
COSN30513622 36 COSMIC
COSN20062191 58 COSMIC
COSN31604802 135 COSMIC
COSN20107200 210 COSMIC
COSN28841817 210 COSMIC
COSN6980625 210 COSMIC
COSN1121589 211 COSMIC
COSN20077007 212 COSMIC
COSN31539791 213 COSMIC
COSN20070812 221 COSMIC
COSN20107203 265 COSMIC
COSN30170725 310 COSMIC
COSN31566799 411 COSMIC
COSN32064473 459 COSMIC
COSN31540988 529 COSMIC
COSN31585313 754 COSMIC
COSN8188758 798 COSMIC
COSN31490745 818 COSMIC
COSN31603089 890 COSMIC
COSN20107205 1111 COSMIC
COSN31589279 1135 COSMIC
COSN31609006 1180 COSMIC
COSN31539141 1378 COSMIC
COSN24303541 1453 COSMIC
COSN26566228 1525 COSMIC
COSN8718660 1602 COSMIC
COSN31548837 1615 COSMIC
COSN31569908 1658 COSMIC
COSN16757669 1700 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1419864328 1 dbSNP
rs200260415 3 dbSNP
rs771845835 5 dbSNP
rs1161156736 6 dbSNP
rs371219709 6 dbSNP
rs779860991 12 dbSNP
rs1364293350 17 dbSNP
rs1368485086 18 dbSNP
rs1465535055 22 dbSNP
rs746639568 26 dbSNP
rs1322496849 31 dbSNP
rs374434524 34 dbSNP
rs746106660 36 dbSNP
rs776381105 40 dbSNP
rs1308313045 48 dbSNP
rs1314886090 50 dbSNP
rs201389290 51 dbSNP
rs199743893 57 dbSNP
rs965060387 65 dbSNP
rs972818557 82 dbSNP
rs200896218 84 dbSNP
rs7897909 85 dbSNP
rs1156494499 86 dbSNP
rs34799098 87 dbSNP
rs1196668940 92 dbSNP
rs984036138 94 dbSNP
rs909517775 98 dbSNP
rs939792852 103 dbSNP
rs1263190491 107 dbSNP
rs1224759521 113 dbSNP
rs927138978 114 dbSNP
rs199593653 116 dbSNP
rs937170523 119 dbSNP
rs1436505362 120 dbSNP
rs992635504 123 dbSNP
rs1445186073 128 dbSNP
rs1337782112 133 dbSNP
rs1400148112 133 dbSNP
rs1337997848 136 dbSNP
rs1416176004 136 dbSNP
rs1423167753 138 dbSNP
rs1294780772 139 dbSNP
rs1172200670 147 dbSNP
rs182005192 158 dbSNP
rs1404277183 159 dbSNP
rs1176591666 161 dbSNP
rs933811025 177 dbSNP
rs1288958783 178 dbSNP
rs1243633703 181 dbSNP
rs949413348 186 dbSNP
rs1330910451 188 dbSNP
rs1221928177 193 dbSNP
rs1223967393 194 dbSNP
rs1282407334 194 dbSNP
rs1306660520 195 dbSNP
rs1352460859 195 dbSNP
rs1238381306 196 dbSNP
rs563006523 196 dbSNP
rs1316567275 197 dbSNP
rs1157111978 198 dbSNP
rs1333638358 198 dbSNP
rs1362634165 198 dbSNP
rs1401477928 198 dbSNP
rs1438791674 198 dbSNP
rs61666042 198 dbSNP
rs867976031 201 dbSNP
rs80173774 208 dbSNP
rs1183525580 209 dbSNP
rs1215820838 209 dbSNP
rs1236362563 209 dbSNP
rs1491562286 209 dbSNP
rs1230495873 210 dbSNP
rs1252475540 210 dbSNP
rs1284143804 210 dbSNP
rs1377682831 210 dbSNP
rs145705395 210 dbSNP
rs201568954 210 dbSNP
rs397965848 210 dbSNP
rs902495408 210 dbSNP
rs2394445 211 dbSNP
rs2394446 212 dbSNP
rs1238971686 213 dbSNP
rs571452063 213 dbSNP
rs1271172273 214 dbSNP
rs1365934184 214 dbSNP
rs1166723028 215 dbSNP
rs1489894484 217 dbSNP
rs936950010 218 dbSNP
rs1054057440 219 dbSNP
rs1478484714 220 dbSNP
rs1260485400 221 dbSNP
rs201471665 222 dbSNP
rs1490422780 223 dbSNP
rs1049707560 224 dbSNP
rs1266380559 224 dbSNP
rs1224169703 227 dbSNP
rs1320322039 228 dbSNP
rs200076477 229 dbSNP
rs1215677411 230 dbSNP
rs1175865989 231 dbSNP
rs1342449988 235 dbSNP
rs534141040 237 dbSNP
rs1007015362 239 dbSNP
rs761619405 247 dbSNP
rs1399612296 252 dbSNP
rs1041162404 255 dbSNP
rs1164880121 257 dbSNP
rs1010796388 259 dbSNP
rs1349106847 259 dbSNP
rs533281509 260 dbSNP
rs71468897 260 dbSNP
rs34333992 261 dbSNP
rs550817042 261 dbSNP
rs1363137795 262 dbSNP
rs201234481 263 dbSNP
rs202117558 264 dbSNP
rs1467180433 265 dbSNP
rs1021649923 277 dbSNP
rs1420793596 279 dbSNP
rs1423492693 282 dbSNP
rs1308555906 284 dbSNP
rs771266253 284 dbSNP
rs1169401132 291 dbSNP
rs1352321588 294 dbSNP
rs1439675909 295 dbSNP
rs553340036 298 dbSNP
rs1025753815 301 dbSNP
rs199910730 307 dbSNP
rs1005601641 310 dbSNP
rs201038526 314 dbSNP
rs1218049007 316 dbSNP
rs1032157738 319 dbSNP
rs1034285782 321 dbSNP
rs566956017 324 dbSNP
rs202131824 326 dbSNP
rs1366636934 327 dbSNP
rs1298574797 333 dbSNP
rs1448873061 339 dbSNP
rs539698204 341 dbSNP
rs200463924 346 dbSNP
rs1275907981 350 dbSNP
rs1440066692 355 dbSNP
rs1202820823 357 dbSNP
rs200906110 366 dbSNP
rs960267376 367 dbSNP
rs992666711 372 dbSNP
rs1432279767 377 dbSNP
rs773118531 378 dbSNP
rs1424879229 379 dbSNP
rs1179603024 382 dbSNP
rs1185015003 383 dbSNP
rs1198587630 387 dbSNP
rs1236815586 387 dbSNP
rs202022771 390 dbSNP
rs1383070748 393 dbSNP
rs1257901728 396 dbSNP
rs1443466426 402 dbSNP
rs917077815 403 dbSNP
rs971258276 407 dbSNP
rs200484021 408 dbSNP
rs1241992570 409 dbSNP
rs1374385683 417 dbSNP
rs978189446 418 dbSNP
rs1396592254 424 dbSNP
rs1403684749 426 dbSNP
rs1300623498 429 dbSNP
rs1398460625 434 dbSNP
rs146210245 438 dbSNP
rs1408163424 440 dbSNP
rs1465131954 446 dbSNP
rs1345380669 448 dbSNP
rs1023093683 455 dbSNP
rs1160091955 457 dbSNP
rs970290494 465 dbSNP
rs923994229 467 dbSNP
rs1359494198 469 dbSNP
rs1178566725 470 dbSNP
rs780441408 471 dbSNP
rs201540418 472 dbSNP
rs1317415520 478 dbSNP
rs4746720 481 dbSNP
rs191683727 487 dbSNP
rs972491441 495 dbSNP
rs1298466541 500 dbSNP
rs757848882 503 dbSNP
rs768510467 508 dbSNP
rs1046824300 512 dbSNP
rs544488472 514 dbSNP
rs1299638094 527 dbSNP
rs372049342 528 dbSNP
rs933718192 534 dbSNP
rs1443344818 543 dbSNP
rs1158472210 545 dbSNP
rs558383162 560 dbSNP
rs578185204 563 dbSNP
rs1370662731 565 dbSNP
rs540963858 566 dbSNP
rs889600713 582 dbSNP
rs560484573 584 dbSNP
rs1240820675 586 dbSNP
rs183459104 589 dbSNP
rs1264066654 593 dbSNP
rs570842400 603 dbSNP
rs1196108720 608 dbSNP
rs201141942 609 dbSNP
rs1043644972 634 dbSNP
rs904550899 637 dbSNP
rs1358207609 641 dbSNP
rs1263549403 643 dbSNP
rs1460599573 654 dbSNP
rs199763427 661 dbSNP
rs1279589585 666 dbSNP
rs200693736 669 dbSNP
rs34629106 671 dbSNP
rs1374272459 672 dbSNP
rs1053247059 673 dbSNP
rs1387634092 676 dbSNP
rs34905990 681 dbSNP
rs1474165075 688 dbSNP
rs201834907 690 dbSNP
rs199595233 694 dbSNP
rs1014305999 698 dbSNP
rs1172847367 704 dbSNP
rs1023567458 705 dbSNP
rs752578 711 dbSNP
rs1199873388 715 dbSNP
rs201845782 716 dbSNP
rs1024174964 719 dbSNP
rs967251703 721 dbSNP
rs977636215 725 dbSNP
rs1017133221 728 dbSNP
rs753028333 729 dbSNP
rs200216073 740 dbSNP
rs1316749626 741 dbSNP
rs200926423 743 dbSNP
rs751532857 748 dbSNP
rs1470802470 749 dbSNP
rs1334455605 752 dbSNP
rs531483546 757 dbSNP
rs1235032919 766 dbSNP
rs201735082 774 dbSNP
rs200055074 779 dbSNP
rs201190294 788 dbSNP
rs922261075 796 dbSNP
rs747696902 800 dbSNP
rs1440659613 808 dbSNP
rs1401612519 812 dbSNP
rs1333958018 823 dbSNP
rs202100731 824 dbSNP
rs200320170 828 dbSNP
rs1464077353 830 dbSNP
rs201212022 830 dbSNP
rs190285590 832 dbSNP
rs202101696 837 dbSNP
rs1328241050 843 dbSNP
rs1479153293 846 dbSNP
rs200384759 848 dbSNP
rs942979857 849 dbSNP
rs370599712 859 dbSNP
rs955315136 869 dbSNP
rs1225416604 872 dbSNP
rs201052021 875 dbSNP
rs985205689 877 dbSNP
rs199656966 883 dbSNP
rs1197836636 888 dbSNP
rs1270113131 890 dbSNP
rs911079465 893 dbSNP
rs200290279 895 dbSNP
rs1196684626 899 dbSNP
rs1225658445 908 dbSNP
rs182180876 918 dbSNP
rs201543666 920 dbSNP
rs1323339276 923 dbSNP
rs1043696375 925 dbSNP
rs527573780 926 dbSNP
rs1233941716 936 dbSNP
rs1047634488 943 dbSNP
rs199502438 946 dbSNP
rs1191866818 947 dbSNP
rs941097672 948 dbSNP
rs1334992870 950 dbSNP
rs200657230 953 dbSNP
rs1400283204 954 dbSNP
rs1481396597 959 dbSNP
rs201966906 964 dbSNP
rs1440010413 966 dbSNP
rs1382003384 971 dbSNP
rs761866266 983 dbSNP
rs1055507029 990 dbSNP
rs1173930861 992 dbSNP
rs1428306897 998 dbSNP
rs1457606147 1000 dbSNP
rs199737066 1005 dbSNP
rs1237527190 1007 dbSNP
rs1053536855 1008 dbSNP
rs35177771 1013 dbSNP
rs1013901206 1019 dbSNP
rs201624047 1025 dbSNP
rs199940169 1030 dbSNP
rs201034799 1037 dbSNP
rs771897405 1041 dbSNP
rs1354866459 1050 dbSNP
rs201828340 1051 dbSNP
rs200204866 1054 dbSNP
rs773136817 1061 dbSNP
rs906075963 1064 dbSNP
rs1285331910 1065 dbSNP
rs1005674096 1078 dbSNP
rs200979033 1081 dbSNP
rs1450213453 1089 dbSNP
rs202194843 1093 dbSNP
rs200050686 1095 dbSNP
rs200992688 1097 dbSNP
rs1029763367 1099 dbSNP
rs1156663826 1106 dbSNP
rs955048005 1107 dbSNP
rs34934649 1108 dbSNP
rs397897192 1108 dbSNP
rs201649689 1109 dbSNP
rs1323271132 1110 dbSNP
rs200250645 1111 dbSNP
rs1164947094 1118 dbSNP
rs201564904 1122 dbSNP
rs1229458821 1126 dbSNP
rs1471516600 1127 dbSNP
rs202103369 1129 dbSNP
rs1262065759 1130 dbSNP
rs1193901056 1133 dbSNP
rs1489096755 1139 dbSNP
rs1303979434 1140 dbSNP
rs985641195 1142 dbSNP
rs200607418 1143 dbSNP
rs530011019 1150 dbSNP
rs1338935039 1151 dbSNP
rs201340848 1155 dbSNP
rs1290001230 1160 dbSNP
rs1316698489 1162 dbSNP
rs910964998 1166 dbSNP
rs964026126 1168 dbSNP
rs1354330432 1181 dbSNP
rs967911929 1183 dbSNP
rs1197148526 1184 dbSNP
rs1268175445 1186 dbSNP
rs1400809767 1191 dbSNP
rs1342553131 1192 dbSNP
rs186781623 1200 dbSNP
rs922881381 1201 dbSNP
rs930183775 1203 dbSNP
rs1166828415 1206 dbSNP
rs923734755 1211 dbSNP
rs200719936 1213 dbSNP
rs935258303 1224 dbSNP
rs201466008 1228 dbSNP
rs1403577732 1230 dbSNP
rs983002258 1232 dbSNP
rs199830798 1233 dbSNP
rs200653280 1246 dbSNP
rs1420934208 1250 dbSNP
rs1451933543 1250 dbSNP
rs1485736587 1250 dbSNP
rs1247720352 1251 dbSNP
rs555801598 1252 dbSNP
rs201955286 1254 dbSNP
rs200058489 1257 dbSNP
rs910036834 1259 dbSNP
rs941111390 1263 dbSNP
rs1214274494 1264 dbSNP
rs1055567701 1267 dbSNP
rs200960441 1273 dbSNP
rs1392263399 1278 dbSNP
rs201330251 1279 dbSNP
rs1331957174 1285 dbSNP
rs199962286 1285 dbSNP
rs950072403 1286 dbSNP
rs200887491 1292 dbSNP
rs752339078 1298 dbSNP
rs201859026 1301 dbSNP
rs200166513 1303 dbSNP
rs1044916041 1311 dbSNP
rs903134366 1314 dbSNP
rs200985506 1316 dbSNP
rs1433450046 1323 dbSNP
rs202196290 1327 dbSNP
rs200457992 1329 dbSNP
rs201736997 1331 dbSNP
rs999176702 1334 dbSNP
rs201951335 1338 dbSNP
rs1360964757 1342 dbSNP
rs1452998712 1343 dbSNP
rs200236450 1350 dbSNP
rs1199889454 1351 dbSNP
rs1459140854 1353 dbSNP
rs1290870178 1355 dbSNP
rs1483343509 1364 dbSNP
rs1249733156 1365 dbSNP
rs558060918 1367 dbSNP
rs1312947740 1374 dbSNP
rs1231723376 1382 dbSNP
rs1038517919 1388 dbSNP
rs531824364 1392 dbSNP
rs201367882 1394 dbSNP
rs1197290446 1395 dbSNP
rs1309867932 1395 dbSNP
rs1448986287 1404 dbSNP
rs1397456510 1407 dbSNP
rs1297151779 1408 dbSNP
rs199731267 1409 dbSNP
rs1360493154 1411 dbSNP
rs200969334 1414 dbSNP
rs367988652 1415 dbSNP
rs994852183 1416 dbSNP
rs35094409 1418 dbSNP
rs201328132 1421 dbSNP
rs1007317888 1432 dbSNP
rs1394927674 1436 dbSNP
rs1179779339 1437 dbSNP
rs1029898932 1439 dbSNP
rs1249067778 1444 dbSNP
rs1442139607 1444 dbSNP
rs1018331294 1445 dbSNP
rs1181356484 1447 dbSNP
rs1257917106 1448 dbSNP
rs890817698 1451 dbSNP
rs1006553264 1453 dbSNP
rs964058316 1454 dbSNP
rs199594782 1456 dbSNP
rs976729189 1457 dbSNP
rs1030066595 1467 dbSNP
rs1161756522 1478 dbSNP
rs200741530 1479 dbSNP
rs201572592 1480 dbSNP
rs967797546 1484 dbSNP
rs1380784985 1501 dbSNP
rs200174408 1505 dbSNP
rs200505035 1507 dbSNP
rs755850401 1507 dbSNP
rs983419468 1510 dbSNP
rs979146420 1515 dbSNP
rs1462965884 1516 dbSNP
rs201473924 1518 dbSNP
rs941528821 1527 dbSNP
rs1159040328 1530 dbSNP
rs1394747259 1546 dbSNP
rs991617984 1547 dbSNP
rs1186034364 1550 dbSNP
rs1391243578 1551 dbSNP
rs200063906 1553 dbSNP
rs201351145 1554 dbSNP
rs1218469246 1556 dbSNP
rs956547974 1561 dbSNP
rs949776915 1563 dbSNP
rs1260014162 1564 dbSNP
rs1384558008 1565 dbSNP
rs1322968559 1577 dbSNP
rs1290958530 1578 dbSNP
rs1413655004 1579 dbSNP
rs1340707401 1581 dbSNP
rs201634954 1583 dbSNP
rs1288789803 1586 dbSNP
rs992520251 1587 dbSNP
rs199995962 1597 dbSNP
rs1307954972 1598 dbSNP
rs1474938294 1606 dbSNP
rs934626019 1608 dbSNP
rs1054792536 1615 dbSNP
rs566459104 1617 dbSNP
rs1260827619 1618 dbSNP
rs201010747 1619 dbSNP
rs1007347582 1629 dbSNP
rs202127568 1639 dbSNP
rs1249613587 1641 dbSNP
rs1271234328 1643 dbSNP
rs899960930 1644 dbSNP
rs1204077491 1649 dbSNP
rs1279689380 1651 dbSNP
rs1224867521 1668 dbSNP
rs200446075 1669 dbSNP
rs796626168 1677 dbSNP
rs1295370757 1683 dbSNP
rs200886908 1683 dbSNP
rs1387729863 1688 dbSNP
rs1397273416 1698 dbSNP
rs35791720 1701 dbSNP
rs367730817 1701 dbSNP
rs562914481 1702 dbSNP
rs1460931916 1712 dbSNP
rs1401195596 1723 dbSNP
rs2234975 1729 dbSNP
rs1474577966 1739 dbSNP
rs1181781862 1741 dbSNP
rs1199259695 1742 dbSNP
rs1384668409 1744 dbSNP
rs1434694815 1747 dbSNP
rs1251003264 1755 dbSNP
rs1213060462 1756 dbSNP
rs200444800 1757 dbSNP
rs192417939 1759 dbSNP
rs951445538 1776 dbSNP
rs79324200 1777 dbSNP
rs1004195059 1781 dbSNP
rs1017606969 1781 dbSNP
rs748956353 1794 dbSNP
rs1301861528 1797 dbSNP
rs1373664767 1797 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions SNU638 , HEK293
Location of target site 3'UTR
Original Description (Extracted from the article) ... "For a subset of target genes including MET ...

- Bou Kheir T; Futoma-Kazmierczak E; Jacobsen et al., 2011, Molecular cancer.

Article - Bou Kheir T; Futoma-Kazmierczak E; Jacobsen et al.
- Molecular cancer, 2011
BACKGROUND: Gastric cancer is the fourth most common cancer in the world and the second most prevalent cause of cancer related death. The development of gastric cancer is mainly associated with H. Pylori infection leading to a focus in pathology studies on bacterial and environmental factors, and to a lesser extent on the mechanistic development of the tumour. MicroRNAs are small non-coding RNA molecules involved in post-transcriptional gene regulation. They are found to regulate genes involved in diverse biological functions and alterations in microRNA expression have been linked to the pathogenesis of many malignancies. The current study is focused on identifying microRNAs involved in gastric carcinogenesis and to explore their mechanistic relevance by characterizing their targets. RESULTS: Invitrogen NCode miRNA microarrays identified miR-449 to be decreased in 1-year-old Gastrin KO mice and in H. Pylori infected gastric tissues compared to tissues from wild type animals. Growth rate of gastric cell lines over-expressing miR-449 was inhibited by 60% compared to controls. FACS cell cycle analysis of miR-449 over-expressing cells showed a significant increase in the sub-G1 fraction indicative of apoptosis. ss-Gal assays indicated a senescent phenotype of gastric cell lines over-expressing miR-449. Affymetrix 133v2 arrays identified GMNN, MET, CCNE2, SIRT1 and CDK6 as miR-449 targets. Luciferase assays were used to confirm GMNN, MET, CCNE2 and SIRT1 as direct targets. We also show that miR-449 over-expression activated p53 and its downstream target p21 as well as the apoptosis markers cleaved CASP3 and PARP. Importantly, qPCR analyses showed a loss of miR-449 expression in human clinical gastric tumours compared to normal tissues. CONCLUSIONS: In this study, we document a diminished expression of miR-449 in Gastrin KO mice and further confirmed its loss in human gastric tumours. We investigated the function of miR-449 by identifying its direct targets. Furthermore we show that miR-449 induces senescence and apoptosis by activating the p53 pathway.
LinkOut: [PMID: 21418558]
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
GSE19350 CNS germ cell tumors -0.701 5.5e-3 -0.042 4.5e-1 12 Click to see details
GSE21687 Ependynoma primary tumors -0.313 5.9e-3 -0.330 3.9e-3 64 Click to see details
GSE28544 Breast cancer 0.496 6.9e-3 0.436 1.7e-2 24 Click to see details
GSE28260 Renal cortex and medulla 0.472 5.2e-2 0.368 1.1e-1 13 Click to see details
GSE26953 Aortic valvular endothelial cells 0.296 8.0e-2 0.290 8.5e-2 24 Click to see details
GSE27834 Pluripotent stem cells -0.353 9.0e-2 -0.262 1.6e-1 16 Click to see details
GSE19783 ER+ ER+ breast cancer -0.294 1.0e-1 -0.123 3.0e-1 20 Click to see details
GSE38226 Liver fibrosis 0.268 1.2e-1 0.237 1.5e-1 21 Click to see details
GSE19783 ER- ER- breast cancer 0.114 1.6e-1 0.073 2.6e-1 79 Click to see details
GSE38974 Chronic obstructive pulmonary disease -0.197 1.7e-1 -0.086 3.4e-1 25 Click to see details
GSE32688 Pancreatic cancer 0.094 3.0e-1 0.187 1.5e-1 32 Click to see details
GSE21849 B cell lymphoma 0.097 3.1e-1 0.068 3.6e-1 29 Click to see details
GSE19536 Breast cancer 0.047 3.2e-1 0.076 2.3e-1 100 Click to see details
GSE14794 Lymphoblastoid cells 0.047 3.3e-1 0.050 3.2e-1 90 Click to see details
GSE17498 Multiple myeloma -0.059 3.6e-1 0.020 4.5e-1 40 Click to see details
GSE17306 Multiple myeloma 0.04 3.9e-1 0.149 1.5e-1 49 Click to see details
GSE34608 Pulmonary tuberculosis and sarcoidosis -0.047 4.2e-1 -0.540 7.0e-3 20 Click to see details
GSE42095 Differentiated embryonic stem cells -0.04 4.3e-1 0.104 3.2e-1 23 Click to see details
GSE35602 Colorectal cancer stromal tissue -0.038 4.3e-1 0.218 1.5e-1 25 Click to see details
GSE15076 Monocyte-derived dendritic cells -0.01 4.9e-1 0.167 3.5e-1 8 Click to see details
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
THCA 0.912 0.13 1.000 0.5 3 Click to see details
LUSC -0.528 0.24 -0.600 0.2 4 Click to see details
UCEC -0.28 0.25 -0.167 0.35 8 Click to see details
BRCA -0.082 0.46 0.000 0.5 4 Click to see details
BRCA -0.082 0.46 0.000 0.5 4 Click to see details
BRCA -0.082 0.46 0.000 0.5 4 Click to see details
BRCA -0.082 0.46 0.000 0.5 4 Click to see details
BRCA -0.082 0.46 0.000 0.5 4 Click to see details
109 hsa-miR-449b-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT006349 SIRT1 sirtuin 1 2 1
MIRT006350 CCNE2 cyclin E2 4 3
MIRT006351 MET MET proto-oncogene, receptor tyrosine kinase 2 1
MIRT006352 GMNN geminin, DNA replication inhibitor 2 1
MIRT006353 HDAC1 histone deacetylase 1 2 1
MIRT016137 CDK4 cyclin dependent kinase 4 1 1
MIRT016138 CDC25A cell division cycle 25A 2 1
MIRT016139 CDK6 cyclin dependent kinase 6 4 3
MIRT057740 ZDHHC16 zinc finger DHHC-type containing 16 2 2
MIRT078636 FAM104A family with sequence similarity 104 member A 2 2
MIRT100407 HSPA1B heat shock protein family A (Hsp70) member 1B 2 2
MIRT115552 MAZ MYC associated zinc finger protein 2 2
MIRT130146 TXNIP thioredoxin interacting protein 2 4
MIRT142254 DCTN5 dynactin subunit 5 2 2
MIRT143724 CCL22 C-C motif chemokine ligand 22 2 2
MIRT168101 E2F3 E2F transcription factor 3 2 2
MIRT169809 GIGYF1 GRB10 interacting GYF protein 1 2 4
MIRT183694 MDM4 MDM4, p53 regulator 2 2
MIRT198922 SMAD4 SMAD family member 4 2 2
MIRT202950 TSN translin 2 4
MIRT221565 CBX3 chromobox 3 2 2
MIRT253418 EVI5L ecotropic viral integration site 5 like 2 2
MIRT294759 ZNF551 zinc finger protein 551 2 4
MIRT307053 TGFBR2 transforming growth factor beta receptor 2 2 2
MIRT331273 TM9SF3 transmembrane 9 superfamily member 3 2 6
MIRT372293 UBXN2B UBX domain protein 2B 2 2
MIRT374317 MBD6 methyl-CpG binding domain protein 6 2 2
MIRT445817 DNAAF3 dynein axonemal assembly factor 3 2 2
MIRT447158 MFSD8 major facilitator superfamily domain containing 8 2 2
MIRT447823 CTIF cap binding complex dependent translation initiation factor 2 2
MIRT448798 GMFB glia maturation factor beta 2 2
MIRT451580 HIRIP3 HIRA interacting protein 3 2 2
MIRT452679 GPR156 G protein-coupled receptor 156 2 2
MIRT453158 CNOT4 CCR4-NOT transcription complex subunit 4 2 6
MIRT453634 SLC4A2 solute carrier family 4 member 2 2 2
MIRT455384 PLA2G2D phospholipase A2 group IID 2 2
MIRT456958 SPAM1 sperm adhesion molecule 1 2 2
MIRT462739 EFNB1 ephrin B1 2 2
MIRT464321 UST uronyl 2-sulfotransferase 2 2
MIRT465641 TNRC18P2 trinucleotide repeat containing 18 pseudogene 2 2 10
MIRT466748 SYNGR2 synaptogyrin 2 2 2
MIRT468345 SF3B3 splicing factor 3b subunit 3 2 2
MIRT469324 RGP1 RGP1 homolog, RAB6A GEF complex partner 1 2 2
MIRT474361 KMT2D lysine methyltransferase 2D 2 2
MIRT475316 IFNLR1 interferon lambda receptor 1 2 2
MIRT477759 EDEM3 ER degradation enhancing alpha-mannosidase like protein 3 2 2
MIRT477824 DYRK3 dual specificity tyrosine phosphorylation regulated kinase 3 2 2
MIRT478265 DDX19B DEAD-box helicase 19B 2 2
MIRT481042 BAZ2A bromodomain adjacent to zinc finger domain 2A 2 2
MIRT481289 ATXN1L ataxin 1 like 2 2
MIRT482730 COPZ1 coatomer protein complex subunit zeta 1 2 2
MIRT488780 CYTH3 cytohesin 3 2 2
MIRT489578 SSBP2 single stranded DNA binding protein 2 2 2
MIRT489659 SHMT1 serine hydroxymethyltransferase 1 2 4
MIRT490527 KIAA1715 lunapark, ER junction formation factor 2 2
MIRT492949 NEUROD2 neuronal differentiation 2 2 2
MIRT493154 MKNK2 MAP kinase interacting serine/threonine kinase 2 2 2
MIRT493845 FOXN3 forkhead box N3 2 4
MIRT494743 ARHGAP1 Rho GTPase activating protein 1 2 6
MIRT496491 MAST3 microtubule associated serine/threonine kinase 3 2 2
MIRT503312 FICD FIC domain containing 2 4
MIRT503507 ZNF623 zinc finger protein 623 2 2
MIRT504182 FAM127B retrotransposon Gag like 8A 2 2
MIRT505010 ZNF644 zinc finger protein 644 2 2
MIRT505352 TMEM167A transmembrane protein 167A 2 2
MIRT505645 SHOC2 SHOC2, leucine rich repeat scaffold protein 2 2
MIRT505710 SESN2 sestrin 2 2 2
MIRT505777 SATB2 SATB homeobox 2 2 6
MIRT506208 PHF19 PHD finger protein 19 2 2
MIRT506251 PEG10 paternally expressed 10 2 2
MIRT507786 CDKN1B cyclin dependent kinase inhibitor 1B 2 2
MIRT508005 BCL2L13 BCL2 like 13 2 4
MIRT508857 ZRSR1 zinc finger CCCH-type, RNA binding motif and serine/arginine rich 1 2 4
MIRT510546 XBP1P1 X-box binding protein 1 pseudogene 1 2 4
MIRT511892 GAS1 growth arrest specific 1 2 6
MIRT512907 UBL4A ubiquitin like 4A 2 2
MIRT513600 VPS37B VPS37B, ESCRT-I subunit 2 2
MIRT521241 SAR1A secretion associated Ras related GTPase 1A 2 2
MIRT521321 RRAGD Ras related GTP binding D 2 4
MIRT525281 C18orf32 chromosome 18 open reading frame 32 2 2
MIRT528650 RWDD2A RWD domain containing 2A 2 4
MIRT535101 PODXL podocalyxin like 2 2
MIRT547752 KBTBD6 kelch repeat and BTB domain containing 6 2 4
MIRT547865 HSPA13 heat shock protein family A (Hsp70) member 13 2 2
MIRT549170 BMP3 bone morphogenetic protein 3 2 2
MIRT550115 SLC35G2 solute carrier family 35 member G2 2 2
MIRT556097 MOAP1 modulator of apoptosis 1 2 2
MIRT557172 HOXA13 homeobox A13 2 2
MIRT557422 GXYLT2 glucoside xylosyltransferase 2 2 2
MIRT557931 FAM73A mitoguardin 1 2 2
MIRT568804 VPS37D VPS37D, ESCRT-I subunit 2 2
MIRT571326 TPCN2 two pore segment channel 2 2 2
MIRT572598 PAPLN papilin, proteoglycan like sulfated glycoprotein 2 2
MIRT573437 APOPT1 apoptogenic 1, mitochondrial 2 2
MIRT574092 VASN vasorin 2 2
MIRT608688 VAV3 vav guanine nucleotide exchange factor 3 2 4
MIRT608800 ITGA11 integrin subunit alpha 11 2 4
MIRT610332 SSX5 SSX family member 5 2 2
MIRT613158 DDA1 DET1 and DDB1 associated 1 2 2
MIRT625407 CPEB3 cytoplasmic polyadenylation element binding protein 3 2 2
MIRT636603 CLIC5 chloride intracellular channel 5 2 2
MIRT638573 IER5 immediate early response 5 2 2
MIRT639686 AASDHPPT aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase 2 2
MIRT677979 ITGB3 integrin subunit beta 3 2 2
MIRT685217 POTED POTE ankyrin domain family member D 2 2
MIRT702570 JARID2 jumonji and AT-rich interaction domain containing 2 2 2
MIRT706259 MKLN1 muskelin 1 2 2
MIRT734675 HMGB1 high mobility group box 1 3 0
MIRT736369 HSPA1A heat shock protein family A (Hsp70) member 1A 1 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-449b Glucose NULL 5793 Quantitative real-time PCR endothelial cells 24394957 2014 down-regulated
miR-449b Doxorubicin approved 31703 Quantitative real-time PCR ovarian carcinoma cell line A2780 cells 22340095 2012 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-449b Ceritinib 57379345 NSC776422 approved sensitive High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-mir-449b Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-miR-449b-5p Gemcitabine 60750 NSC613327 approved sensitive High Pancreatic Cancer cell line (SUIT-2, CAPAN-1)
hsa-miR-449b-5p Oxaliplatin 6857599 NSC266046 approved resistant High Colorectal Cancer cell line (RKO)
hsa-miR-449b-5p Oxaliplatin 6857599 NSC266046 approved sensitive High Colorectal Cancer cell line (HCT-116)
hsa-miR-449b-5p Ceritinib 57379345 NSC776422 approved sensitive High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-449b-5p Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-449b-5p Panobinostat 6918837 NSC761190 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-449b-5p Cisplatin 5460033 NSC119875 approved resistant High Gastric Cancer tissue
hsa-miR-449b-5p Fluorouracil 3385 NSC19893 approved resistant High Gastric Cancer tissue
hsa-miR-449b-5p Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-449b-5p Vemurafenib 42611257 NSC761431 approved resistant cell line (451Lu)
hsa-miR-449b-5p Paclitaxel 36314 NSC125973 approved sensitive cell line (HeyA8)
hsa-miR-449b-5p Paclitaxel 36314 NSC125973 approved resistant cell line (SKVO3ip1)
hsa-miR-449b-5p Fluorouracil 3385 NSC19893 approved resistant cell line (KM12C) (72 h)
hsa-miR-449b-5p Platinum 23939 resistant tissue (non-small cell lung cancer)
hsa-miR-449b-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-449b-5p Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-miR-449b-5p Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide/Methotrexate/Gemcitabine resistant cell line (Bats-72)
hsa-miR-449b-5p Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide resistant cell line (Bads-200)

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