miRTarBase - #MIRT006351


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pre-miRNA Information
pre-miRNA hsa-mir-449b   
Genomic Coordinates chr5: 55170646 - 55170742
Description Homo sapiens miR-449b stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-449b-5p
Sequence 16| AGGCAGUGUAUUGUUAGCUGGC |37
Evidence Experimental
Experiments Microarray
DRVs in miRNA
Mutant ID Mutant Position Mutant Source
COSN8871076 12 COSMIC
COSN17153496 22 COSMIC
COSN31576772 22 COSMIC
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1322925717 3 dbSNP
rs769526811 10 dbSNP
rs10061133 12 dbSNP
rs1431829587 13 dbSNP
rs778042202 21 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol MET   
Synonyms AUTS9, DFNB97, HGFR, RCCP2, c-Met
Description MET proto-oncogene, receptor tyrosine kinase
Transcript NM_000245   
Other Transcripts NM_001127500   
Expression
Putative miRNA Targets on MET
3'UTR of MET
(miRNA target sites are highlighted)		 
>MET|NM_000245|3'UTR
   1 TGCTAGTACTATGTCAAAGCAACAGTCCACACTTTGTCCAATGGTTTTTTCACTGCCTGACCTTTAAAAGGCCATCGATA
  81 TTCTTTGCTCTTGCCAAAATTGCACTATTATAGGACTTGTATTGTTATTTAAATTACTGGATTCTAAGGAATTTCTTATC
 161 TGACAGAGCATCAGAACCAGAGGCTTGGTCCCACAGGCCACGGACCAATGGCCTGCAGCCGTGACAACACTCCTGTCATA
 241 TTGGAGTCCAAAACTTGAATTCTGGGTTGAATTTTTTAAAAATCAGGTACCACTTGATTTCATATGGGAAATTGAAGCAG
 321 GAAATATTGAGGGCTTCTTGATCACAGAAAACTCAGAAGAGATAGTAATGCTCAGGACAGGAGCGGCAGCCCCAGAACAG
 401 GCCACTCATTTAGAATTCTAGTGTTTCAAAACACTTTTGTGTGTTGTATGGTCAATAACATTTTTCATTACTGATGGTGT
 481 CATTCACCCATTAGGTAAACATTCCCTTTTAAATGTTTGTTTGTTTTTTGAGACAGGATCTCACTCTGTTGCCAGGGCTG
 561 TAGTGCAGTGGTGTGATCATAGCTCACTGCAACCTCCACCTCCCAGGCTCAAGCCTCCCGAATAGCTGGGACTACAGGCG
 641 CACACCACCATCCCCGGCTAATTTTTGTATTTTTTGTAGAGACGGGGTTTTGCCATGTTGCCAAGGCTGGTTTCAAACTC
 721 CTGGACTCAAGAAATCCACCCACCTCAGCCTCCCAAAGTGCTAGGATTACAGGCATGAGCCACTGCGCCCAGCCCTTATA
 801 AATTTTTGTATAGACATTCCTTTGGTTGGAAGAATATTTATAGGCAATACAGTCAAAGTTTCAAAATAGCATCACACAAA
 881 ACATGTTTATAAATGAACAGGATGTAATGTACATAGATGACATTAAGAAAATTTGTATGAAATAATTTAGTCATCATGAA
 961 ATATTTAGTTGTCATATAAAAACCCACTGTTTGAGAATGATGCTACTCTGATCTAATGAATGTGAACATGTAGATGTTTT
1041 GTGTGTATTTTTTTAAATGAAAACTCAAAATAAGACAAGTAATTTGTTGATAAATATTTTTAAAGATAACTCAGCATGTT
1121 TGTAAAGCAGGATACATTTTACTAAAAGGTTCATTGGTTCCAATCACAGCTCATAGGTAGAGCAAAGAAAGGGTGGATGG
1201 ATTGAAAAGATTAGCCTCTGTCTCGGTGGCAGGTTCCCACCTCGCAAGCAATTGGAAACAAAACTTTTGGGGAGTTTTAT
1281 TTTGCATTAGGGTGTGTTTTATGTTAAGCAAAACATACTTTAGAAACAAATGAAAAAGGCAATTGAAAATCCCAGCTATT
1361 TCACCTAGATGGAATAGCCACCCTGAGCAGAACTTTGTGATGCTTCATTCTGTGGAATTTTGTGCTTGCTACTGTATAGT
1441 GCATGTGGTGTAGGTTACTCTAACTGGTTTTGTCGACGTAAACATTTAAAGTGTTATATTTTTTATAAAAATGTTTATTT
1521 TTAATGATATGAGAAAAATTTTGTTAGGCCACAAAAACACTGCACTGTGAACATTTTAGAAAAGGTATGTCAGACTGGGA
1601 TTAATGACAGCATGATTTTCAATGACTGTAAATTGCGATAAGGAAATGTACTGATTGCCAATACACCCCACCCTCATTAC
1681 ATCATCAGGACTTGAAGCCAAGGGTTAACCCAGCAAGCTACAAAGAGGGTGTGTCACACTGAAACTCAATAGTTGAGTTT
1761 GGCTGTTGTTGCAGGAAAATGATTATAACTAAAAGCTCTCTGATAGTGCAGAGACTTACCAGAAGACACAAGGAATTGTA
1841 CTGAAGAGCTATTACAATCCAAATATTGCCGTTTCATAAATGTAATAAGTAATACTAATTCACAGAGTATTGTAAATGGT
1921 GGATGACAAAAGAAAATCTGCTCTGTGGAAAGAAAGAACTGTCTCTACCAGGGTCAAGAGCATGAACGCATCAATAGAAA
2001 GAACTCGGGGAAACATCCCATCAACAGGACTACACACTTGTATATACATTCTTGAGAACACTGCAATGTGAAAATCACGT
2081 TTGCTATTTATAAACTTGTCCTTAGATTAATGTGTCTGGACAGATTGTGGGAGTAAGTGATTCTTCTAAGAATTAGATAC
2161 TTGTCACTGCCTATACCTGCAGCTGAACTGAATGGTACTTCGTATGTTAATAGTTGTTCTGATAAATCATGCAATTAAAG
2241 TAAAGTGATGCAACATCTTGTAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cgGUCGAUUGUUAUGUGACGGa 5'
            :|| || : :| ||||||| 
Target 5' atTAGATACTTGT-CACTGCCt 3'
 2152 - 2172 151.00 -16.10
2 
miRNA  3' cggucGAUUGUU----AUGUGACGGa 5'
               | ||:::    | ||||||| 
Target 5' tttgtCCAATGGTTTTTTCACTGCCt 3'
 33 - 58 143.00 -15.05
3 
miRNA  3' cggUCGAUUGUUAUGUGACGga 5'
             :|| |  || |||||||  
Target 5' ttaGGCCACAAAAACACTGCac 3'
 1544 - 1565 139.00 -13.10
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
908057 3 ClinVar
1200759 21 ClinVar
908058 28 ClinVar
358695 42 ClinVar
358696 77 ClinVar
358697 88 ClinVar
358698 106 ClinVar
910012 202 ClinVar
358699 217 ClinVar
358700 222 ClinVar
358701 232 ClinVar
910013 297 ClinVar
910014 360 ClinVar
910015 377 ClinVar
358702 386 ClinVar
910901 398 ClinVar
358703 423 ClinVar
910902 439 ClinVar
910903 481 ClinVar
910904 494 ClinVar
910905 531 ClinVar
358704 603 ClinVar
358705 656 ClinVar
358706 657 ClinVar
358707 685 ClinVar
912133 787 ClinVar
358708 788 ClinVar
912134 804 ClinVar
358709 945 ClinVar
358710 985 ClinVar
358711 996 ClinVar
358712 1011 ClinVar
358713 1029 ClinVar
358714 1221 ClinVar
358715 1225 ClinVar
908120 1234 ClinVar
908121 1245 ClinVar
908122 1245 ClinVar
358716 1327 ClinVar
910070 1360 ClinVar
358717 1429 ClinVar
358718 1461 ClinVar
910071 1478 ClinVar
358719 1594 ClinVar
358720 1637 ClinVar
910072 1638 ClinVar
910966 1715 ClinVar
358721 1785 ClinVar
358722 1810 ClinVar
910967 1872 ClinVar
910968 1879 ClinVar
358723 1883 ClinVar
358724 1887 ClinVar
910969 1910 ClinVar
912196 1930 ClinVar
358725 1988 ClinVar
358726 2052 ClinVar
358727 2080 ClinVar
912197 2101 ClinVar
358728 2135 ClinVar
912198 2148 ClinVar
358729 2203 ClinVar
908191 2203 ClinVar
358730 2241 ClinVar
908192 2246 ClinVar
COSN31572651 20 COSMIC
COSN28671489 21 COSMIC
COSN26984213 25 COSMIC
COSN30673660 32 COSMIC
COSN16913096 43 COSMIC
COSN30472788 107 COSMIC
COSN28294506 149 COSMIC
COSN30490027 150 COSMIC
COSN18719176 220 COSMIC
COSN29142494 319 COSMIC
COSN26465647 440 COSMIC
COSN26555722 516 COSMIC
COSN25463416 581 COSMIC
COSN23557458 631 COSMIC
COSN27049349 659 COSMIC
COSN9920401 824 COSMIC
COSN31484060 833 COSMIC
COSN21241771 1206 COSMIC
COSN31544353 1238 COSMIC
COSN28755990 1327 COSMIC
COSN30334834 1403 COSMIC
COSN25997721 1914 COSMIC
COSN21218048 1937 COSMIC
COSN17183421 1980 COSMIC
COSN4864725 2005 COSMIC
COSN6346070 2102 COSMIC
COSN26579863 2171 COSMIC
COSN31519892 2219 COSMIC
COSN28724667 2241 COSMIC
rs1621 1429 GWAS
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1243543318 1 dbSNP
rs200573684 17 dbSNP
rs751784236 20 dbSNP
rs757592643 21 dbSNP
rs980940721 24 dbSNP
rs781614353 27 dbSNP
rs750811600 33 dbSNP
rs780355204 34 dbSNP
rs901963499 36 dbSNP
rs1295455580 37 dbSNP
rs749821599 38 dbSNP
rs996580734 40 dbSNP
rs771518812 42 dbSNP
rs777311092 46 dbSNP
rs1212950991 53 dbSNP
rs560878054 72 dbSNP
rs941757308 73 dbSNP
rs1447616518 74 dbSNP
rs527496385 77 dbSNP
rs1334504424 80 dbSNP
rs41281081 88 dbSNP
rs918925719 89 dbSNP
rs929233874 103 dbSNP
rs1032910944 105 dbSNP
rs886061945 106 dbSNP
rs1421028269 108 dbSNP
rs1203634586 114 dbSNP
rs1261079833 120 dbSNP
rs1186556354 124 dbSNP
rs955855423 125 dbSNP
rs757750209 127 dbSNP
rs1195995476 131 dbSNP
rs1206015285 138 dbSNP
rs1462042758 150 dbSNP
rs1251052402 169 dbSNP
rs1203041185 175 dbSNP
rs1309737976 182 dbSNP
rs916983613 184 dbSNP
rs1415579387 187 dbSNP
rs1051665582 189 dbSNP
rs890347972 192 dbSNP
rs541441794 193 dbSNP
rs1038825040 195 dbSNP
rs1165307486 201 dbSNP
rs1129355 202 dbSNP
rs980266955 203 dbSNP
rs1287897336 209 dbSNP
rs926949147 210 dbSNP
rs1443450013 213 dbSNP
rs537781991 216 dbSNP
rs149188493 217 dbSNP
rs1444795762 220 dbSNP
rs1310613010 221 dbSNP
rs886061946 222 dbSNP
rs59399612 232 dbSNP
rs1221926046 239 dbSNP
rs1357350458 242 dbSNP
rs1233219875 262 dbSNP
rs1293964085 266 dbSNP
rs1247000152 282 dbSNP
rs148370379 284 dbSNP
rs902013283 286 dbSNP
rs1306054463 287 dbSNP
rs1323910749 292 dbSNP
rs1237383281 297 dbSNP
rs1002793749 302 dbSNP
rs1260339214 303 dbSNP
rs996243551 305 dbSNP
rs1363174219 312 dbSNP
rs1164890885 313 dbSNP
rs1354775472 328 dbSNP
rs1218802139 343 dbSNP
rs1033847172 344 dbSNP
rs1157423377 349 dbSNP
rs1421243179 362 dbSNP
rs546444252 363 dbSNP
rs560298133 364 dbSNP
rs1199341480 365 dbSNP
rs1489985110 373 dbSNP
rs553602374 377 dbSNP
rs573358625 380 dbSNP
rs963496991 385 dbSNP
rs181068390 386 dbSNP
rs1236701900 393 dbSNP
rs1318492672 394 dbSNP
rs1288686098 396 dbSNP
rs559231345 398 dbSNP
rs1364013265 402 dbSNP
rs746413882 408 dbSNP
rs886061947 423 dbSNP
rs918956919 424 dbSNP
rs577605241 427 dbSNP
rs1156431640 430 dbSNP
rs955990225 439 dbSNP
rs1413162402 442 dbSNP
rs544970146 448 dbSNP
rs1479757854 449 dbSNP
rs1432057531 465 dbSNP
rs112249479 467 dbSNP
rs1200283033 475 dbSNP
rs772411950 477 dbSNP
rs1259985584 478 dbSNP
rs1207133647 481 dbSNP
rs1486293799 488 dbSNP
rs987113308 495 dbSNP
rs1222648684 499 dbSNP
rs1321373360 506 dbSNP
rs1285107313 509 dbSNP
rs774490932 514 dbSNP
rs1414661984 520 dbSNP
rs1315520596 522 dbSNP
rs1303903534 529 dbSNP
rs911801861 530 dbSNP
rs185897782 531 dbSNP
rs1024108081 533 dbSNP
rs574748666 535 dbSNP
rs1173658238 539 dbSNP
rs1475341195 555 dbSNP
rs1039313707 556 dbSNP
rs1195357465 558 dbSNP
rs542271812 559 dbSNP
rs143338232 561 dbSNP
rs936782478 562 dbSNP
rs759653192 568 dbSNP
rs1390016918 571 dbSNP
rs1264706454 576 dbSNP
rs1200965661 578 dbSNP
rs951676943 580 dbSNP
rs1267924842 584 dbSNP
rs1306743022 592 dbSNP
rs1228951837 595 dbSNP
rs1365527871 598 dbSNP
rs147343288 603 dbSNP
rs908124464 608 dbSNP
rs1046356799 609 dbSNP
rs1408776595 610 dbSNP
rs940886110 620 dbSNP
rs1041035833 621 dbSNP
rs1394940728 624 dbSNP
rs1169763656 626 dbSNP
rs1430481480 628 dbSNP
rs1210977485 629 dbSNP
rs923436472 636 dbSNP
rs1002455394 639 dbSNP
rs191068881 641 dbSNP
rs1050516424 650 dbSNP
rs1437220287 652 dbSNP
rs563978117 656 dbSNP
rs531456758 657 dbSNP
rs1026089493 677 dbSNP
rs1191390259 680 dbSNP
rs1198780945 683 dbSNP
rs987144308 684 dbSNP
rs12540703 685 dbSNP
rs1334578917 686 dbSNP
rs1331441848 689 dbSNP
rs965026667 694 dbSNP
rs1161703269 696 dbSNP
rs1426808306 697 dbSNP
rs1414776945 707 dbSNP
rs1434775529 708 dbSNP
rs974669584 715 dbSNP
rs1469436690 719 dbSNP
rs1231165337 721 dbSNP
rs1184278682 732 dbSNP
rs1483304067 737 dbSNP
rs1267597200 742 dbSNP
rs892474045 748 dbSNP
rs1219592208 759 dbSNP
rs1010078383 776 dbSNP
rs1323185444 778 dbSNP
rs1024659888 780 dbSNP
rs1290139738 785 dbSNP
rs935854365 787 dbSNP
rs143472935 788 dbSNP
rs913994505 795 dbSNP
rs1001801655 804 dbSNP
rs1345377535 812 dbSNP
rs535127764 817 dbSNP
rs1406817029 818 dbSNP
rs1350755691 819 dbSNP
rs1317895159 826 dbSNP
rs547400075 829 dbSNP
rs1405530230 830 dbSNP
rs1034092517 832 dbSNP
rs1301503021 837 dbSNP
rs1161056019 843 dbSNP
rs1370609151 867 dbSNP
rs951813236 870 dbSNP
rs765058024 871 dbSNP
rs545051967 872 dbSNP
rs1196740251 874 dbSNP
rs984544465 877 dbSNP
rs1246723457 878 dbSNP
rs945454632 879 dbSNP
rs1444880948 890 dbSNP
rs1046869285 913 dbSNP
rs1229367145 914 dbSNP
rs772971653 919 dbSNP
rs961721103 937 dbSNP
rs976338867 942 dbSNP
rs565768785 945 dbSNP
rs1269602807 951 dbSNP
rs1428926640 954 dbSNP
rs1347135941 973 dbSNP
rs1322344567 975 dbSNP
rs573510587 985 dbSNP
rs995008616 988 dbSNP
rs1211639393 989 dbSNP
rs1481637404 990 dbSNP
rs1026451594 993 dbSNP
rs886061948 996 dbSNP
rs1425296429 997 dbSNP
rs1185376042 998 dbSNP
rs1251972255 999 dbSNP
rs150534017 1011 dbSNP
rs1051066910 1012 dbSNP
rs1461137727 1025 dbSNP
rs41738 1029 dbSNP
rs538497590 1042 dbSNP
rs752823191 1046 dbSNP
rs756086234 1048 dbSNP
rs936723909 1056 dbSNP
rs1272404368 1058 dbSNP
rs764256174 1081 dbSNP
rs892441506 1090 dbSNP
rs1212858866 1092 dbSNP
rs1401728449 1094 dbSNP
rs868142960 1106 dbSNP
rs1298590534 1107 dbSNP
rs1452115032 1108 dbSNP
rs1467502349 1111 dbSNP
rs1324380384 1115 dbSNP
rs964396014 1116 dbSNP
rs974789945 1130 dbSNP
rs1014026471 1131 dbSNP
rs1032893138 1144 dbSNP
rs1045636106 1145 dbSNP
rs1169179300 1149 dbSNP
rs1393939095 1151 dbSNP
rs907146102 1157 dbSNP
rs1001371751 1158 dbSNP
rs1188824082 1162 dbSNP
rs1443564966 1170 dbSNP
rs1183914142 1171 dbSNP
rs754179332 1178 dbSNP
rs1483893840 1189 dbSNP
rs181818408 1200 dbSNP
rs757521023 1201 dbSNP
rs913063432 1203 dbSNP
rs1344426944 1205 dbSNP
rs1275357431 1210 dbSNP
rs1276638458 1216 dbSNP
rs139535303 1221 dbSNP
rs149713628 1225 dbSNP
rs1397525801 1226 dbSNP
rs928007892 1232 dbSNP
rs560568639 1234 dbSNP
rs1297527450 1235 dbSNP
rs938046205 1242 dbSNP
rs1005947737 1244 dbSNP
rs144559852 1245 dbSNP
rs1382983595 1246 dbSNP
rs1272320534 1249 dbSNP
rs1424745032 1256 dbSNP
rs1451771202 1258 dbSNP
rs1055498170 1260 dbSNP
rs899424650 1266 dbSNP
rs1240041843 1267 dbSNP
rs1484299385 1270 dbSNP
rs1252649634 1282 dbSNP
rs930874808 1289 dbSNP
rs1489931768 1293 dbSNP
rs1485249108 1296 dbSNP
rs1193828928 1302 dbSNP
rs1424967818 1312 dbSNP
rs1479092984 1317 dbSNP
rs545938651 1323 dbSNP
rs961687972 1325 dbSNP
rs41739 1327 dbSNP
rs886588667 1328 dbSNP
rs1030575569 1331 dbSNP
rs531420311 1334 dbSNP
rs543673515 1335 dbSNP
rs1308162642 1339 dbSNP
rs371968912 1340 dbSNP
rs1399463760 1349 dbSNP
rs1298950610 1357 dbSNP
rs1342223243 1361 dbSNP
rs758949656 1369 dbSNP
rs1458035670 1373 dbSNP
rs1363615406 1376 dbSNP
rs1157309729 1386 dbSNP
rs75361918 1389 dbSNP
rs542456294 1394 dbSNP
rs1413298285 1401 dbSNP
rs936859381 1408 dbSNP
rs900210693 1422 dbSNP
rs1489877229 1428 dbSNP
rs1621 1429 dbSNP
rs1485644444 1430 dbSNP
rs1259950066 1435 dbSNP
rs568734054 1451 dbSNP
rs1032630282 1455 dbSNP
rs957321826 1459 dbSNP
rs886061949 1461 dbSNP
rs1222758859 1462 dbSNP
rs1356749515 1468 dbSNP
rs1229870432 1472 dbSNP
rs1302713204 1475 dbSNP
rs1387014911 1476 dbSNP
rs750893461 1478 dbSNP
rs547071825 1479 dbSNP
rs905192814 1485 dbSNP
rs534473251 1486 dbSNP
rs988668429 1489 dbSNP
rs1176356843 1512 dbSNP
rs937223581 1515 dbSNP
rs1379482761 1528 dbSNP
rs1199615955 1529 dbSNP
rs1216619781 1534 dbSNP
rs1020100182 1539 dbSNP
rs1055736429 1544 dbSNP
rs1466079200 1550 dbSNP
rs1398098105 1556 dbSNP
rs971266153 1566 dbSNP
rs887587790 1574 dbSNP
rs747538243 1579 dbSNP
rs1342725448 1593 dbSNP
rs764929802 1594 dbSNP
rs1233295872 1606 dbSNP
rs565728956 1609 dbSNP
rs779688649 1611 dbSNP
rs928075396 1620 dbSNP
rs1366386319 1623 dbSNP
rs1336444482 1626 dbSNP
rs938061303 1629 dbSNP
rs186175651 1637 dbSNP
rs534514626 1638 dbSNP
rs562396709 1646 dbSNP
rs1030372302 1648 dbSNP
rs1190758534 1655 dbSNP
rs1450279142 1658 dbSNP
rs1047996544 1665 dbSNP
rs1182727638 1671 dbSNP
rs1440666097 1672 dbSNP
rs551508074 1676 dbSNP
rs953086220 1681 dbSNP
rs1200590288 1682 dbSNP
rs1338193883 1688 dbSNP
rs1362573189 1689 dbSNP
rs1432983845 1693 dbSNP
rs1271778906 1694 dbSNP
rs571020696 1698 dbSNP
rs747850853 1715 dbSNP
rs1341057509 1726 dbSNP
rs1449952552 1733 dbSNP
rs944895498 1739 dbSNP
rs769390271 1744 dbSNP
rs1032579261 1750 dbSNP
rs958186057 1754 dbSNP
rs1335435278 1762 dbSNP
rs369765654 1774 dbSNP
rs554029534 1785 dbSNP
rs1170511553 1803 dbSNP
rs868554436 1810 dbSNP
rs1430698695 1811 dbSNP
rs1384804925 1814 dbSNP
rs773019048 1815 dbSNP
rs1345075643 1816 dbSNP
rs1209586598 1819 dbSNP
rs913983341 1822 dbSNP
rs1278519265 1828 dbSNP
rs949457882 1830 dbSNP
rs1369919389 1832 dbSNP
rs1188173431 1837 dbSNP
rs982228909 1848 dbSNP
rs1482666895 1849 dbSNP
rs1249017576 1851 dbSNP
rs372830179 1852 dbSNP
rs1189967753 1860 dbSNP
rs1483201933 1860 dbSNP
rs556742174 1871 dbSNP
rs139377965 1872 dbSNP
rs770910579 1879 dbSNP
rs886061950 1883 dbSNP
rs536185931 1887 dbSNP
rs1238985908 1895 dbSNP
rs1349150963 1905 dbSNP
rs1009826853 1912 dbSNP
rs1410506607 1918 dbSNP
rs941835625 1920 dbSNP
rs1365247381 1925 dbSNP
rs1321841621 1930 dbSNP
rs869105634 1933 dbSNP
rs553745206 1936 dbSNP
rs1415736844 1943 dbSNP
rs1163577342 1982 dbSNP
rs1472254090 1984 dbSNP
rs1020173809 1987 dbSNP
rs76322625 1988 dbSNP
rs1172138511 1989 dbSNP
rs1034591613 1994 dbSNP
rs1218936621 1998 dbSNP
rs1402746532 2001 dbSNP
rs545860655 2007 dbSNP
rs557851750 2008 dbSNP
rs577416382 2009 dbSNP
rs920624124 2011 dbSNP
rs1229487223 2015 dbSNP
rs1344969402 2021 dbSNP
rs1273114533 2029 dbSNP
rs1438908777 2030 dbSNP
rs1399308676 2043 dbSNP
rs1447294010 2045 dbSNP
rs1310964979 2049 dbSNP
rs886061951 2052 dbSNP
rs1386434810 2054 dbSNP
rs1161302101 2061 dbSNP
rs1368400781 2065 dbSNP
rs759512297 2068 dbSNP
rs1231883271 2074 dbSNP
rs1423881085 2078 dbSNP
rs1188924741 2079 dbSNP
rs757859772 2080 dbSNP
rs1246922287 2083 dbSNP
rs190739281 2101 dbSNP
rs1040585823 2105 dbSNP
rs1270332386 2115 dbSNP
rs1209937129 2119 dbSNP
rs144156652 2135 dbSNP
rs922074233 2138 dbSNP
rs1220990290 2157 dbSNP
rs1368661115 2158 dbSNP
rs1269814098 2160 dbSNP
rs768452050 2176 dbSNP
rs1433097066 2187 dbSNP
rs958319884 2197 dbSNP
rs1009774931 2202 dbSNP
rs14456 2203 dbSNP
rs892861856 2205 dbSNP
rs753944287 2206 dbSNP
rs1358471387 2213 dbSNP
rs1173424922 2228 dbSNP
rs6566 2241 dbSNP
rs1425183711 2242 dbSNP
rs1167572256 2245 dbSNP
rs1447240969 2246 dbSNP
rs755000162 2247 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions SNU638 , HEK293
Location of target site 3'UTR
Original Description (Extracted from the article) ... "For a subset of target genes including MET ...

- Bou Kheir T; Futoma-Kazmierczak E; Jacobsen et al., 2011, Molecular cancer.

Article - Bou Kheir T; Futoma-Kazmierczak E; Jacobsen et al.
- Molecular cancer, 2011
BACKGROUND: Gastric cancer is the fourth most common cancer in the world and the second most prevalent cause of cancer related death. The development of gastric cancer is mainly associated with H. Pylori infection leading to a focus in pathology studies on bacterial and environmental factors, and to a lesser extent on the mechanistic development of the tumour. MicroRNAs are small non-coding RNA molecules involved in post-transcriptional gene regulation. They are found to regulate genes involved in diverse biological functions and alterations in microRNA expression have been linked to the pathogenesis of many malignancies. The current study is focused on identifying microRNAs involved in gastric carcinogenesis and to explore their mechanistic relevance by characterizing their targets. RESULTS: Invitrogen NCode miRNA microarrays identified miR-449 to be decreased in 1-year-old Gastrin KO mice and in H. Pylori infected gastric tissues compared to tissues from wild type animals. Growth rate of gastric cell lines over-expressing miR-449 was inhibited by 60% compared to controls. FACS cell cycle analysis of miR-449 over-expressing cells showed a significant increase in the sub-G1 fraction indicative of apoptosis. ss-Gal assays indicated a senescent phenotype of gastric cell lines over-expressing miR-449. Affymetrix 133v2 arrays identified GMNN, MET, CCNE2, SIRT1 and CDK6 as miR-449 targets. Luciferase assays were used to confirm GMNN, MET, CCNE2 and SIRT1 as direct targets. We also show that miR-449 over-expression activated p53 and its downstream target p21 as well as the apoptosis markers cleaved CASP3 and PARP. Importantly, qPCR analyses showed a loss of miR-449 expression in human clinical gastric tumours compared to normal tissues. CONCLUSIONS: In this study, we document a diminished expression of miR-449 in Gastrin KO mice and further confirmed its loss in human gastric tumours. We investigated the function of miR-449 by identifying its direct targets. Furthermore we show that miR-449 induces senescence and apoptosis by activating the p53 pathway.
LinkOut: [PMID: 21418558]
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
GSE19783 ER- ER- breast cancer -0.384 2.4e-4 -0.397 1.5e-4 79 Click to see details
GSE28544 Breast cancer -0.608 8.1e-4 -0.656 2.5e-4 24 Click to see details
GSE28260 Renal cortex and medulla 0.741 1.9e-3 0.687 4.7e-3 13 Click to see details
GSE19536 Breast cancer -0.262 4.2e-3 -0.274 2.9e-3 100 Click to see details
GSE27834 Pluripotent stem cells -0.511 2.2e-2 -0.506 2.3e-2 16 Click to see details
GSE42095 Differentiated embryonic stem cells -0.329 6.3e-2 -0.031 4.4e-1 23 Click to see details
GSE38226 Liver fibrosis 0.33 7.2e-2 0.300 9.3e-2 21 Click to see details
GSE17498 Multiple myeloma 0.133 2.1e-1 0.024 4.4e-1 40 Click to see details
GSE21849 B cell lymphoma 0.144 2.3e-1 0.459 6.1e-3 29 Click to see details
GSE19783 ER+ ER+ breast cancer 0.167 2.4e-1 0.242 1.5e-1 20 Click to see details
GSE17306 Multiple myeloma -0.101 2.4e-1 0.101 2.4e-1 49 Click to see details
GSE15076 Monocyte-derived dendritic cells -0.264 2.6e-1 -0.262 2.7e-1 8 Click to see details
GSE14794 Lymphoblastoid cells -0.056 3.0e-1 -0.040 3.5e-1 90 Click to see details
GSE26953 Aortic valvular endothelial cells -0.092 3.3e-1 0.017 4.7e-1 24 Click to see details
GSE35602 Colorectal cancer stromal tissue -0.083 3.5e-1 0.054 4.0e-1 25 Click to see details
GSE19350 CNS germ cell tumors -0.108 3.7e-1 -0.014 4.8e-1 12 Click to see details
GSE21687 Ependynoma primary tumors -0.038 3.8e-1 -0.019 4.4e-1 64 Click to see details
GSE34608 Pulmonary tuberculosis and sarcoidosis 0.066 3.9e-1 -0.341 7.1e-2 20 Click to see details
GSE32688 Pancreatic cancer -0.046 4.0e-1 -0.052 3.9e-1 32 Click to see details
GSE38974 Chronic obstructive pulmonary disease 0.045 4.2e-1 0.154 2.3e-1 25 Click to see details
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
LUSC -0.308 0.35 -0.600 0.2 4 Click to see details
BRCA -0.302 0.35 -0.400 0.3 4 Click to see details
UCEC 0.088 0.42 0.143 0.37 8 Click to see details
THCA 0.161 0.45 0.500 0.33 3 Click to see details
THCA 0.161 0.45 0.500 0.33 3 Click to see details
THCA 0.161 0.45 0.500 0.33 3 Click to see details
THCA 0.161 0.45 0.500 0.33 3 Click to see details
THCA 0.161 0.45 0.500 0.33 3 Click to see details
109 hsa-miR-449b-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT006349 SIRT1 sirtuin 1 2 1
MIRT006350 CCNE2 cyclin E2 4 3
MIRT006351 MET MET proto-oncogene, receptor tyrosine kinase 2 1
MIRT006352 GMNN geminin, DNA replication inhibitor 2 1
MIRT006353 HDAC1 histone deacetylase 1 2 1
MIRT016137 CDK4 cyclin dependent kinase 4 1 1
MIRT016138 CDC25A cell division cycle 25A 2 1
MIRT016139 CDK6 cyclin dependent kinase 6 4 3
MIRT057740 ZDHHC16 zinc finger DHHC-type containing 16 2 2
MIRT078636 FAM104A family with sequence similarity 104 member A 2 2
MIRT100407 HSPA1B heat shock protein family A (Hsp70) member 1B 2 2
MIRT115552 MAZ MYC associated zinc finger protein 2 2
MIRT130146 TXNIP thioredoxin interacting protein 2 4
MIRT142254 DCTN5 dynactin subunit 5 2 2
MIRT143724 CCL22 C-C motif chemokine ligand 22 2 2
MIRT168101 E2F3 E2F transcription factor 3 2 2
MIRT169809 GIGYF1 GRB10 interacting GYF protein 1 2 4
MIRT183694 MDM4 MDM4, p53 regulator 2 2
MIRT198922 SMAD4 SMAD family member 4 2 2
MIRT202950 TSN translin 2 4
MIRT221565 CBX3 chromobox 3 2 2
MIRT253418 EVI5L ecotropic viral integration site 5 like 2 2
MIRT294759 ZNF551 zinc finger protein 551 2 4
MIRT307053 TGFBR2 transforming growth factor beta receptor 2 2 2
MIRT331273 TM9SF3 transmembrane 9 superfamily member 3 2 6
MIRT372293 UBXN2B UBX domain protein 2B 2 2
MIRT374317 MBD6 methyl-CpG binding domain protein 6 2 2
MIRT445817 DNAAF3 dynein axonemal assembly factor 3 2 2
MIRT447158 MFSD8 major facilitator superfamily domain containing 8 2 2
MIRT447823 CTIF cap binding complex dependent translation initiation factor 2 2
MIRT448798 GMFB glia maturation factor beta 2 2
MIRT451580 HIRIP3 HIRA interacting protein 3 2 2
MIRT452679 GPR156 G protein-coupled receptor 156 2 2
MIRT453158 CNOT4 CCR4-NOT transcription complex subunit 4 2 6
MIRT453634 SLC4A2 solute carrier family 4 member 2 2 2
MIRT455384 PLA2G2D phospholipase A2 group IID 2 2
MIRT456958 SPAM1 sperm adhesion molecule 1 2 2
MIRT462739 EFNB1 ephrin B1 2 2
MIRT464321 UST uronyl 2-sulfotransferase 2 2
MIRT465641 TNRC18P2 trinucleotide repeat containing 18 pseudogene 2 2 10
MIRT466748 SYNGR2 synaptogyrin 2 2 2
MIRT468345 SF3B3 splicing factor 3b subunit 3 2 2
MIRT469324 RGP1 RGP1 homolog, RAB6A GEF complex partner 1 2 2
MIRT474361 KMT2D lysine methyltransferase 2D 2 2
MIRT475316 IFNLR1 interferon lambda receptor 1 2 2
MIRT477759 EDEM3 ER degradation enhancing alpha-mannosidase like protein 3 2 2
MIRT477824 DYRK3 dual specificity tyrosine phosphorylation regulated kinase 3 2 2
MIRT478265 DDX19B DEAD-box helicase 19B 2 2
MIRT481042 BAZ2A bromodomain adjacent to zinc finger domain 2A 2 2
MIRT481289 ATXN1L ataxin 1 like 2 2
MIRT482730 COPZ1 coatomer protein complex subunit zeta 1 2 2
MIRT488780 CYTH3 cytohesin 3 2 2
MIRT489578 SSBP2 single stranded DNA binding protein 2 2 2
MIRT489659 SHMT1 serine hydroxymethyltransferase 1 2 4
MIRT490527 KIAA1715 lunapark, ER junction formation factor 2 2
MIRT492949 NEUROD2 neuronal differentiation 2 2 2
MIRT493154 MKNK2 MAP kinase interacting serine/threonine kinase 2 2 2
MIRT493845 FOXN3 forkhead box N3 2 4
MIRT494743 ARHGAP1 Rho GTPase activating protein 1 2 6
MIRT496491 MAST3 microtubule associated serine/threonine kinase 3 2 2
MIRT503312 FICD FIC domain containing 2 4
MIRT503507 ZNF623 zinc finger protein 623 2 2
MIRT504182 FAM127B retrotransposon Gag like 8A 2 2
MIRT505010 ZNF644 zinc finger protein 644 2 2
MIRT505352 TMEM167A transmembrane protein 167A 2 2
MIRT505645 SHOC2 SHOC2, leucine rich repeat scaffold protein 2 2
MIRT505710 SESN2 sestrin 2 2 2
MIRT505777 SATB2 SATB homeobox 2 2 6
MIRT506208 PHF19 PHD finger protein 19 2 2
MIRT506251 PEG10 paternally expressed 10 2 2
MIRT507786 CDKN1B cyclin dependent kinase inhibitor 1B 2 2
MIRT508005 BCL2L13 BCL2 like 13 2 4
MIRT508857 ZRSR1 zinc finger CCCH-type, RNA binding motif and serine/arginine rich 1 2 4
MIRT510546 XBP1P1 X-box binding protein 1 pseudogene 1 2 4
MIRT511892 GAS1 growth arrest specific 1 2 6
MIRT512907 UBL4A ubiquitin like 4A 2 2
MIRT513600 VPS37B VPS37B, ESCRT-I subunit 2 2
MIRT521241 SAR1A secretion associated Ras related GTPase 1A 2 2
MIRT521321 RRAGD Ras related GTP binding D 2 4
MIRT525281 C18orf32 chromosome 18 open reading frame 32 2 2
MIRT528650 RWDD2A RWD domain containing 2A 2 4
MIRT535101 PODXL podocalyxin like 2 2
MIRT547752 KBTBD6 kelch repeat and BTB domain containing 6 2 4
MIRT547865 HSPA13 heat shock protein family A (Hsp70) member 13 2 2
MIRT549170 BMP3 bone morphogenetic protein 3 2 2
MIRT550115 SLC35G2 solute carrier family 35 member G2 2 2
MIRT556097 MOAP1 modulator of apoptosis 1 2 2
MIRT557172 HOXA13 homeobox A13 2 2
MIRT557422 GXYLT2 glucoside xylosyltransferase 2 2 2
MIRT557931 FAM73A mitoguardin 1 2 2
MIRT568804 VPS37D VPS37D, ESCRT-I subunit 2 2
MIRT571326 TPCN2 two pore segment channel 2 2 2
MIRT572598 PAPLN papilin, proteoglycan like sulfated glycoprotein 2 2
MIRT573437 APOPT1 apoptogenic 1, mitochondrial 2 2
MIRT574092 VASN vasorin 2 2
MIRT608688 VAV3 vav guanine nucleotide exchange factor 3 2 4
MIRT608800 ITGA11 integrin subunit alpha 11 2 4
MIRT610332 SSX5 SSX family member 5 2 2
MIRT613158 DDA1 DET1 and DDB1 associated 1 2 2
MIRT625407 CPEB3 cytoplasmic polyadenylation element binding protein 3 2 2
MIRT636603 CLIC5 chloride intracellular channel 5 2 2
MIRT638573 IER5 immediate early response 5 2 2
MIRT639686 AASDHPPT aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase 2 2
MIRT677979 ITGB3 integrin subunit beta 3 2 2
MIRT685217 POTED POTE ankyrin domain family member D 2 2
MIRT702570 JARID2 jumonji and AT-rich interaction domain containing 2 2 2
MIRT706259 MKLN1 muskelin 1 2 2
MIRT734675 HMGB1 high mobility group box 1 3 0
MIRT736369 HSPA1A heat shock protein family A (Hsp70) member 1A 1 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-449b Glucose NULL 5793 Quantitative real-time PCR endothelial cells 24394957 2014 down-regulated
miR-449b Doxorubicin approved 31703 Quantitative real-time PCR ovarian carcinoma cell line A2780 cells 22340095 2012 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-449b Ceritinib 57379345 NSC776422 approved sensitive High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-mir-449b Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-miR-449b-5p Gemcitabine 60750 NSC613327 approved sensitive High Pancreatic Cancer cell line (SUIT-2, CAPAN-1)
hsa-miR-449b-5p Oxaliplatin 6857599 NSC266046 approved resistant High Colorectal Cancer cell line (RKO)
hsa-miR-449b-5p Oxaliplatin 6857599 NSC266046 approved sensitive High Colorectal Cancer cell line (HCT-116)
hsa-miR-449b-5p Ceritinib 57379345 NSC776422 approved sensitive High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-449b-5p Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-449b-5p Panobinostat 6918837 NSC761190 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-449b-5p Cisplatin 5460033 NSC119875 approved resistant High Gastric Cancer tissue
hsa-miR-449b-5p Fluorouracil 3385 NSC19893 approved resistant High Gastric Cancer tissue
hsa-miR-449b-5p Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-449b-5p Vemurafenib 42611257 NSC761431 approved resistant cell line (451Lu)
hsa-miR-449b-5p Paclitaxel 36314 NSC125973 approved sensitive cell line (HeyA8)
hsa-miR-449b-5p Paclitaxel 36314 NSC125973 approved resistant cell line (SKVO3ip1)
hsa-miR-449b-5p Fluorouracil 3385 NSC19893 approved resistant cell line (KM12C) (72 h)
hsa-miR-449b-5p Platinum 23939 resistant tissue (non-small cell lung cancer)
hsa-miR-449b-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-449b-5p Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-miR-449b-5p Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide/Methotrexate/Gemcitabine resistant cell line (Bats-72)
hsa-miR-449b-5p Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide resistant cell line (Bads-200)

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