>OTUB1|NM_017670|3'UTR
   1 GGCTGGCTCCAGCCCGCTGCTGCCCTGCTGCCCCCCTCTGCCAGGCGCTAGACATGTACAGAGGTTTTTCTGTGGTTGTA
  81 AATGGTCCTATTTCACCCCCTTCTTCCTGTCACATGACCCCCCCCCATGTTTTATTAAAGGGGGTGCTGGTGGTGAGCCG
 161 TGTGTGCGTGTCCCTGCTCTGCTGCCCGCCTGGCTGCTCTGTCTGCTGCCCCCTCCCCCCAGGTGGGTCCCCCTGCTTTT
 241 CACCTATCTACTCCTGAGCTTCCCCAACAGGAGCAGGTTTGAGGGGCCAGGCCTCTTGGAGGCCCCTCCTGCTTCGTTGG
 321 GTTCTGCTTCCTTCCCTTCTTAGCTGGCTCAGGGGCTTCTATGGGATCCTGGAAGTTCCTTAGGGACTTGCCCAGGGTCC
 401 CAGGGCCACCCACACTTCATCTGCTCCCTCATAGGCCCCACCTCCACGTCCCGGCTGGGCCCCAGACCCCAGCTTCCTGC
 481 CCTCCACCGGGAGTCTGCATGGTTGGGAGTCCTGGGTGGAGGGGCCTTTGTGAGGCTGGACCCGGCTCAGGGCAGGTGGA
 561 GGAGCTGGGCCTCCCACAGGGTGCCCGGGCAGTGCCATCCTGGTGGGGGAGGGCAGCCTTCAAACGTGTGGGGTCTACAG
 641 TCCTCAGGTCTAGGCAGGGCTGCCGGTTCTCCACCTCCCCATCCGCCCCAGGCCCCCTGCCTGTGCCTGCCTTGCACCCC
 721 CTCTGCTTGGGCCACGGTGTCTCTGCATTGCCTGCCTTTTTGCCTTCACCTCTTTTCTTCCCCGCCCCCTGCACATTCGG
 801 GGTCTCAGCCCCCAGGCTGTGAGCTCCTTGGGGGCAGGCCCTCAATAAATGTGAACTGCTGCTGCCGCCTCTGCAAAAAA
 881 AAAAAAAAAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' gaucuucGAGUGUCAGAUCaac 5' |||| :|||||| Target 5' tacagtcCTCA-GGTCTAGgca 3'	636 - 656	118.00	-11.52
2	miRNA 3' gaUCUUCGAGUGUCAGAUCAAc 5' |||:|:|: : | |:||| Target 5' acAGAGGTTT-TTCTGTGGTTg 3'	58 - 78	107.00	-11.70
3	miRNA 3' gaucuuCGAGUGUCAGAUCAAc 5' || | | |||: || Target 5' tgccttGCACCCCCTCTGCTTg 3'	708 - 729	100.00	-5.50

Mutant ID	Mutant Position	Mutant Source
COSN30478388	25	COSMIC
COSN26987682	46	COSMIC
COSN30144328	82	COSMIC
COSN30108676	89	COSMIC
COSN30161162	107	COSMIC
COSN30112455	112	COSMIC
COSN509855	127	COSMIC
COSN30552782	168	COSMIC
COSN22608092	216	COSMIC
COSN1080355	230	COSMIC
COSN27227860	473	COSMIC
COSN31481087	485	COSMIC
COSN28807151	672	COSMIC
COSN28755177	697	COSMIC
COSN31588803	840	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs776958806	3	dbSNP
rs773483542	6	dbSNP
rs1207596350	15	dbSNP
rs375063755	16	dbSNP
rs760868231	16	dbSNP
rs746137458	17	dbSNP
rs758973863	17	dbSNP
rs766486922	17	dbSNP
rs997364635	18	dbSNP
rs769977704	27	dbSNP
rs1488450230	28	dbSNP
rs370933976	31	dbSNP
rs753983822	31	dbSNP
rs763301304	32	dbSNP
rs764672942	35	dbSNP
rs1477342238	36	dbSNP
rs1053383	39	dbSNP
rs552928353	47	dbSNP
rs1471755430	48	dbSNP
rs753467887	51	dbSNP
rs781314211	56	dbSNP
rs1222914913	59	dbSNP
rs1053384	62	dbSNP
rs566483037	64	dbSNP
rs1318874417	74	dbSNP
rs1015523974	89	dbSNP
rs1216626612	91	dbSNP
rs1041965141	95	dbSNP
rs759485891	96	dbSNP
rs765234884	96	dbSNP
rs754683223	97	dbSNP
rs764727163	98	dbSNP
rs535517488	99	dbSNP
rs555298563	100	dbSNP
rs3209355	104	dbSNP
rs752571431	104	dbSNP
rs757051140	115	dbSNP
rs1465686019	117	dbSNP
rs781187157	117	dbSNP
rs1474653404	118	dbSNP
rs35556442	118	dbSNP
rs35611728	118	dbSNP
rs533490120	118	dbSNP
rs959222172	119	dbSNP
rs1283093679	120	dbSNP
rs746083572	122	dbSNP
rs770144495	123	dbSNP
rs575478020	124	dbSNP
rs1198299570	127	dbSNP
rs372981070	127	dbSNP
rs917639985	130	dbSNP
rs749406153	135	dbSNP
rs1257998174	136	dbSNP
rs1440699823	138	dbSNP
rs1491006858	139	dbSNP
rs769006797	140	dbSNP
rs780657767	140	dbSNP
rs1441569111	141	dbSNP
rs1006014792	142	dbSNP
rs1379961393	143	dbSNP
rs966350290	149	dbSNP
rs1338699569	151	dbSNP
rs112341603	159	dbSNP
rs544528368	160	dbSNP
rs770224696	161	dbSNP
rs558039727	163	dbSNP
rs1330084624	164	dbSNP
rs577996825	168	dbSNP
rs1448400886	169	dbSNP
rs1390928483	170	dbSNP
rs1306425244	171	dbSNP
rs1164200341	173	dbSNP
rs1352599994	174	dbSNP
rs1233519259	178	dbSNP
rs1257548634	179	dbSNP
rs897505875	180	dbSNP
rs1342546594	186	dbSNP
rs993136780	188	dbSNP
rs1238673430	189	dbSNP
rs1176480356	193	dbSNP
rs1459011455	193	dbSNP
rs1440808661	196	dbSNP
rs1176907489	197	dbSNP
rs1237633303	198	dbSNP
rs1377066519	200	dbSNP
rs1433986061	202	dbSNP
rs1179681858	203	dbSNP
rs1464470373	204	dbSNP
rs1178431952	207	dbSNP
rs1376306910	209	dbSNP
rs1465816833	210	dbSNP
rs759126650	214	dbSNP
rs1403143927	215	dbSNP
rs924940220	216	dbSNP
rs1328776219	218	dbSNP
rs1375434434	221	dbSNP
rs1387010295	222	dbSNP
rs1030683450	223	dbSNP
rs545918979	224	dbSNP
rs1329166879	227	dbSNP
rs1199168911	228	dbSNP
rs1232313157	229	dbSNP
rs55951521	230	dbSNP
rs1319994745	231	dbSNP
rs528607400	233	dbSNP
rs1017475595	234	dbSNP
rs757892062	242	dbSNP
rs763558263	244	dbSNP
rs921463819	244	dbSNP
rs1051199652	247	dbSNP
rs891176840	249	dbSNP
rs1265581232	253	dbSNP
rs1432794482	257	dbSNP
rs751498385	263	dbSNP
rs1382633467	266	dbSNP
rs1426225028	267	dbSNP
rs914629897	271	dbSNP
rs1464526081	272	dbSNP
rs1469679601	276	dbSNP
rs1247560975	282	dbSNP
rs967370411	288	dbSNP
rs542279886	289	dbSNP
rs1267942582	290	dbSNP
rs1216993309	293	dbSNP
rs1489231610	298	dbSNP
rs1341303598	303	dbSNP
rs1282055587	304	dbSNP
rs1222763109	316	dbSNP
rs1037070263	317	dbSNP
rs1276071110	318	dbSNP
rs1230639938	337	dbSNP
rs1344417479	343	dbSNP
rs898476250	345	dbSNP
rs995857337	354	dbSNP
rs928612850	356	dbSNP
rs938982062	362	dbSNP
rs1400234116	370	dbSNP
rs1425999387	372	dbSNP
rs1209227534	375	dbSNP
rs1242293841	383	dbSNP
rs1476209363	386	dbSNP
rs1056448391	392	dbSNP
rs1479586153	399	dbSNP
rs1013379614	403	dbSNP
rs1389023447	405	dbSNP
rs1024685094	411	dbSNP
rs1454377144	416	dbSNP
rs1174806466	419	dbSNP
rs1248049190	422	dbSNP
rs916084666	436	dbSNP
rs1442887778	440	dbSNP
rs942143040	440	dbSNP
rs1280146687	443	dbSNP
rs374487504	446	dbSNP
rs1201164003	447	dbSNP
rs3802940	448	dbSNP
rs977807252	449	dbSNP
rs3802939	453	dbSNP
rs1333945251	454	dbSNP
rs1268746252	456	dbSNP
rs1430671559	460	dbSNP
rs746797387	460	dbSNP
rs1166452220	461	dbSNP
rs1468292244	463	dbSNP
rs957716761	463	dbSNP
rs1405696194	466	dbSNP
rs1171619164	469	dbSNP
rs1437568194	471	dbSNP
rs993229608	484	dbSNP
rs1185659886	488	dbSNP
rs796718192	489	dbSNP
rs76826727	490	dbSNP
rs1235169107	497	dbSNP
rs373062787	499	dbSNP
rs1258350172	500	dbSNP
rs1342816723	507	dbSNP
rs534025853	508	dbSNP
rs940109534	510	dbSNP
rs1284701821	513	dbSNP
rs550865540	524	dbSNP
rs1037104638	525	dbSNP
rs1206421170	542	dbSNP
rs1450599392	543	dbSNP
rs1247098123	544	dbSNP
rs564485257	545	dbSNP
rs34901004	551	dbSNP
rs1169568096	562	dbSNP
rs1405726914	565	dbSNP
rs1415939010	569	dbSNP
rs1161564037	575	dbSNP
rs1017902759	583	dbSNP
rs1238275020	584	dbSNP
rs1179906595	587	dbSNP
rs112989429	588	dbSNP
rs1231949556	593	dbSNP
rs377502278	598	dbSNP
rs1204332879	600	dbSNP
rs895025990	601	dbSNP
rs1010671010	604	dbSNP
rs1244455819	605	dbSNP
rs1359281886	610	dbSNP
rs1310092279	625	dbSNP
rs1020724963	627	dbSNP
rs1393213934	638	dbSNP
rs533665662	639	dbSNP
rs1297800362	651	dbSNP
rs1409160373	659	dbSNP
rs1351437985	660	dbSNP
rs879229700	663	dbSNP
rs1024802586	665	dbSNP
rs977901578	666	dbSNP
rs147616481	672	dbSNP
rs1160002559	675	dbSNP
rs999261539	676	dbSNP
rs1191379428	680	dbSNP
rs1032024908	682	dbSNP
rs957962464	684	dbSNP
rs747102438	685	dbSNP
rs974395703	686	dbSNP
rs1287357729	687	dbSNP
rs577979511	693	dbSNP
rs1029001836	694	dbSNP
rs566438952	704	dbSNP
rs1232455311	707	dbSNP
rs954354822	714	dbSNP
rs987049564	715	dbSNP
rs912763343	717	dbSNP
rs991764396	718	dbSNP
rs1290531197	721	dbSNP
rs972830363	722	dbSNP
rs919983632	731	dbSNP
rs535214863	733	dbSNP
rs1055164318	735	dbSNP
rs942196599	737	dbSNP
rs1426769766	738	dbSNP
rs1197790950	740	dbSNP
rs895134440	742	dbSNP
rs1335101249	743	dbSNP
rs1053405	744	dbSNP
rs1207444380	748	dbSNP
rs760284878	754	dbSNP
rs549024430	758	dbSNP
rs919414369	769	dbSNP
rs528435392	771	dbSNP
rs1210823239	772	dbSNP
rs1046287510	773	dbSNP
rs1340859636	773	dbSNP
rs569080024	773	dbSNP
rs1051908978	782	dbSNP
rs890133095	784	dbSNP
rs1007606634	785	dbSNP
rs1038968506	788	dbSNP
rs1302993262	795	dbSNP
rs1470665176	796	dbSNP
rs35621932	796	dbSNP
rs1175226586	799	dbSNP
rs893748346	799	dbSNP
rs1010764297	800	dbSNP
rs1063848	805	dbSNP
rs1473764415	809	dbSNP
rs748444085	809	dbSNP
rs1157436115	810	dbSNP
rs73496075	817	dbSNP
rs966514488	823	dbSNP
rs996321313	830	dbSNP
rs998953546	831	dbSNP
rs1203306878	835	dbSNP
rs1318810191	836	dbSNP
rs1252862573	841	dbSNP
rs1224966025	842	dbSNP
rs1165021599	856	dbSNP
rs1325848503	856	dbSNP
rs557975589	857	dbSNP
rs960081755	860	dbSNP
rs954346455	862	dbSNP
rs1008496726	865	dbSNP
rs1447081391	866	dbSNP
rs1019853954	867	dbSNP

- Blood, 2010

BCR-ABL negative myeloproliferative neoplasms (MPNs; polycythemia vera, essential thrombocythemia, primary myelofibrosis) are malignant diseases arising from a multipotent hematopoietic progenitor, frequently altered by JAK2 V617F or other JAK/STAT activating mutations. The thrombopoietin receptor (TpoR, MPL) is one of the major dimeric cytokine receptors that use JAK2 in the myeloid lineage, and was found to be down-modulated in certain MPN patients. We searched for negative regulators of MPL expression. Here we report that miR-28 targets the 3' untranslated (3'UTR) region of MPL, inhibiting its translation, as well as other proteins potentially involved in megakaryocyte differentiation, such as E2F6. Expression of miR-28 in CD34-derived megakaryocytes inhibited terminal differentiation. miR-28 was found to be overexpressed in platelets of a fraction of MPN patients, while it was expressed at constant low levels in platelets from healthy subjects. Constitutive activation of STAT5 leading to autonomous growth of hematopoietic cell lines was associated with increased miR-28 expression. We discuss how down-modulating MPL and other targets of miR-28, and of related miR-708 and miR-151, could contribute to MPN pathogenicity.