miRTarBase - #MIRT006426


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pre-miRNA Information
pre-miRNA mmu-mir-200c   
Genomic Coordinates chr6: 124718322 - 124718390
Description Mus musculus miR-200c stem-loop
Comment Mouse mir-200c is predicted .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-200c-3p
Sequence 45| UAAUACUGCCGGGUAAUGAUGGA |67
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B0Q8FA miR-200c Safety Biomarker (SAF) Clinical/Experimental Data Expression Higher Urine Quantitative polymerase chain reaction
Gene Information
Gene Symbol Mapk14   
Synonyms CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha
Description mitogen-activated protein kinase 14
Transcript NM_001168508   
Other Transcripts NM_001168513 , NM_001168514 , NM_011951   
Expression
Putative miRNA Targets on Mapk14
3'UTR of Mapk14
(miRNA target sites are highlighted)		 
>Mapk14|NM_001168508|3'UTR
   1 GCACCTGGTTTCTGTTCTGTCTATCTCACTTCACTGTGAGGGGAAGACCTTCTCATGGGAACTCTCCAAATACCATTCAA
  81 GTGCCTCTTGTTGAAAGATTCCTTCATGGTGGAAGGGGGTGCATGTATGTGTTAGTGTTTGTGTGTGTGTGTGTGTCTGT
 161 CTGTTCGTCTGTCCACCTATCTTTGTGGAAGTCACTGTGATGGTAGTGACTTTATGAGTTGTGAATGGTCCTTGGCAGTC
 241 TGCCTGCTTTCTCAGAGTCTGGGCAGGCCGATGGGAACTGTCATCTCCTTAGGGATGTGTGTGTTCAGTGCAAAGTAAGA
 321 AATATGAAAATATCCCTGTTCTTAGTTACCTTGCCACTTTGGCTTCTCCTGTGGCCCTGCCTTTACCATATCAGTGACAG
 401 AGAGAGGCTGCTTCAGGTCTGAGGCTATCCCTCAGCCATGCATAAAGTCCAAGAGAACCAACTGGCTCCTGGTCTCTAGC
 481 CTGTGACCGGCTTGCTTAATGTCCTCAGAACCTGACAGGTATGTTCAAAACTGTCAGTCTGTTTGTGCCTTAAAAGGGTG
 561 AGAAGGGCGCGTAGATAGTTACAGAGTCTCAGCTGCTGACGTTCTGAGCCAGGCAAGTGCACGGGGCTGTTGGATGGCCA
 641 GTGGGGAGCTGGAAAAAACAAGGCAGCCTTTAGGAAGGCCATGGTGCATGTGTGTGCATGCGTGTATGTGCAGCCGCCCT
 721 CCCTCACTTCAGGAGCAAGCTGTTTGCTGTGCTTACCCTTCACCTCAGTGCAGAGGTCTCCAGTGCCGAGCACAGGCACC
 801 TGCCATCAGTAGTTCCTGTGTCATCTTCACATCTAGCAGAGCACGGATGTGTTTGCATGCTGTGCTCTTGGAGCTTGTCC
 881 TGTCTTCTGGAAGCCCTGGACAAGGCGTGTGAAGGCTTCCCAGAAGTTCCTGTCCACATTGCCTCCGCCCACCGACGCCA
 961 TGGGCACACTGCTCCCTCCTCCTCCTCCAGCTACTTTGTGTTGAACACAATTGATTCTCCAGGTGCTCATGGTGCAGGAA
1041 AACAGGACAGACAGAGAGCACCTGAACCCTTGCCATCTGATGTCACCAATTCAGGAAAACGAGTCCTCTCCTAGGACTAT
1121 CCCCGGTTCTGGAAATCATGTTCTCCTCACTCATGGTGACAAGCTAAGAAAGCTGAACAAAGGGAGAGACGAGAGCGCCT
1201 GAAGCCAGGAGCTCCTTTACTATCTTTCTCAAAAGGGTTGTTAGACACAAACCAAGTCATCAAGGCCCCGCTCCTCTCCT
1281 CGGAAGGGTCCCCCACCCCCCGGCAGCTTGACACTGAATCCAGTGTCAATTTGGGGAGAAAGCAGTTTTGTCTTGGAATT
1361 TTGTATGTTGTAGGAATCCTTAGAGAGTGTGGTTCCTTCTGATGGGGAGAAAGGGCAAATTATTTTAATATTTTGTATTT
1441 TCACCTTTATAAACATGAATCCTCAGGGGTGAAGAACTGTTTGCATAATTTTCTGAATTTTGAGCACTTTGTGCTATATA
1521 AGGACCCATATTTAAGCTTTGTGTGCAGTAAGAAAGTGTAAAGCCAATTCCAGTGTTGGACGTGACAGGTCTTGTGTTTA
1601 GGTCAAGGTGTCTCCTCTCAGTGCAGGGACATGCCTGCTCTGTGGGGCAGGCGAGGACCCTGAATCATTTGGAGCCCAGA
1681 AGGAGGCAGACTGGCCAGGTCTCACCACCTCAGTGTGCAGTTCAACTCCATGCCATCCCATCAAGATGGGTTAGTAGCAG
1761 TGTCTGTTTTTGAATGCCAAGTGTGATTTCCAACAATTCTGCTCTGGTTATTTCATTGAAGACATCTTTGCACATGTGAC
1841 CATGCTGTGTTAGGGGCTGTGTTCCAGGGACTGGACTCGAAGCTAGAACTGGCAGAAGAGTTCTGGCATCCACAGCGCAA
1921 TGCTGCCACCACCCAGTTTCTTCATCAGAAGACAAGGGAACGAGAAAACTGCTGTTCGTTTGTATTTGTGAACTTGGCTG
2001 TAATCTGGTATGCCATAGGATGTCAGATAATACCACTGGTTAAAATAAAGCCTAGTTTTCAAATTCAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agguaGUA-AUGGGCCGUCAUAAu 5'
               ||| ||: :| :|||:|| 
Target 5' gggtgCATGTATGTGTTAGTGTTt 3'
 117 - 140 126.00 -10.30
2 
miRNA  3' agguaguaaUGGGCCGUCAUAAu 5'
                   |::|  ||||:|| 
Target 5' gtaaagccaATTC--CAGTGTTg 3'
 1558 - 1578 126.00 -9.00
3 
miRNA  3' agguAGUAAUGGGCCGUCAUAau 5'
              |||  |||:  ||||:|  
Target 5' ggtcTCACCACCT--CAGTGTgc 3'
 1698 - 1718 119.00 -9.40
Experimental Support 1 for Non-Functional miRNA-Target Interaction
miRNA:Target xx
Validation Method
Conditions mouse fibroblasts
Original Description (Extracted from the article) ... "Indeed ...

- Mateescu B; Batista L; Cardon M; Gruosso T; et al., 2011, Nature medicine.

Article - Mateescu B; Batista L; Cardon M; Gruosso T; et al.
- Nature medicine, 2011
Although there is evidence that redox regulation has an essential role in malignancies, its impact on tumor prognosis remains unclear. Here we show crosstalk between oxidative stress and the miR-200 family of microRNAs that affects tumorigenesis and chemosensitivity. miR-141 and miR-200a target p38alpha and modulate the oxidative stress response. Enhanced expression of these microRNAs mimics p38alpha deficiency and increases tumor growth in mouse models, but it also improves the response to chemotherapeutic agents. High-grade human ovarian adenocarcinomas that accumulate miR-200a have low concentrations of p38alpha and an associated oxidative stress signature. The miR200a-dependent stress signature correlates with improved survival of patients in response to treatment. Therefore, the role of miR-200a in stress could be a predictive marker for clinical outcome in ovarian cancer. In addition, although oxidative stress promotes tumor growth, it also sensitizes tumors to treatment, which could account for the limited success of antioxidants in clinical trials.
LinkOut: [PMID: 22101765]
33 mmu-miR-200c-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT002336 Zeb1 zinc finger E-box binding homeobox 1 4 3
MIRT002337 Zeb2 zinc finger E-box binding homeobox 2 3 4
MIRT005946 Flt1 FMS-like tyrosine kinase 1 1 1
MIRT006426 Mapk14 mitogen-activated protein kinase 14 2 1
MIRT009810 ZEB1 zinc finger E-box binding homeobox 1 1 1
MIRT009811 ZEB2 zinc finger E-box binding homeobox 2 1 1
MIRT053235 Sox2 SRY (sex determining region Y)-box 2 4 1
MIRT418784 Map2 microtubule-associated protein 2 1 1
MIRT438019 Bmi1 Bmi1 polycomb ring finger oncogene 2 1
MIRT438582 Zfpm2 zinc finger protein, multitype 2 2 1
MIRT438658 Nog noggin 5 1
MIRT577097 Fhod1 formin homology 2 domain containing 1 1 3
MIRT577625 St3gal2 ST3 beta-galactoside alpha-2,3-sialyltransferase 2 1 1
MIRT578593 Hist1h1d histone cluster 1, H1d 1 4
MIRT579059 Cradd CASP2 and RIPK1 domain containing adaptor with death domain 1 1
MIRT581140 Senp5 SUMO/sentrin specific peptidase 5 1 2
MIRT581298 Rnf166 ring finger protein 166 1 1
MIRT581364 Rdh10 retinol dehydrogenase 10 (all-trans) 1 1
MIRT581743 Plxnc1 plexin C1 1 1
MIRT581831 Pkib protein kinase inhibitor beta, cAMP dependent, testis specific 1 1
MIRT582081 Ogfod1 2-oxoglutarate and iron-dependent oxygenase domain containing 1 1 2
MIRT582414 Mphosph9 M-phase phosphoprotein 9 1 2
MIRT582457 Mgat3 mannoside acetylglucosaminyltransferase 3 1 1
MIRT582940 Ikzf5 IKAROS family zinc finger 5 1 1
MIRT583984 Ddx26b integrator complex subunit 6 like 1 1
MIRT595499 Mtf2 metal response element binding transcription factor 2 1 1
MIRT595530 Foxn3 forkhead box N3 1 1
MIRT596310 Rassf10 Ras association (RalGDS/AF-6) domain family (N-terminal) member 10 1 1
MIRT596609 Zfp459 zinc finger protein 459 1 1
MIRT601132 Bri3bp Bri3 binding protein 1 1
MIRT603385 Sh2d4a SH2 domain containing 4A 1 1
MIRT731202 Reln reelin 2 1
MIRT756395 Cd274 CD274 antigen 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-200c All-trans-retinoic acid (ATRA) approved 444796 Quantitative real-time PCR breast cancer cells 23904188 2013 up-regualted
miR-200c Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated
miR-200c Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 up-regulated
miR-200c Trichostatin A (TSA) NULL 444732 Microarray human pancreatic cancer cell line BxPC-3 19112422 2009 up-regulated
miR-200c Trichostatin A (TSA) NULL 444732 Northern blot human pancreatic cancer cell line BxPC-3 19112422 2009 up-regulated
miR-200c 17beta-estradiol (E2) approved 5757 Microarray MCF-7 breast cancer cells 19528081 2009 up-regulated
miR-200c Activin A NULL 229455 Quantitative real-time PCR Human embryonic stem (hES) cells 19885849 2010 up-regulated
miR-200c 5-Fluorouracil approved 3385 Microarray HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-200c CDF(analogues of curcumin) NULL NULL Quantitative real-time PCR pancreatic cancer (gemcitabine-sensitive) BxPC-3 cells and gemcitabine-resistant MIAPaCa-E (relatively resistant to gemcitabine) and MIAPaCa-M (highly resistant to gemcitabine) 20388782 2010 up-regulated
miR-200c CDF(analogues of curcumin) + gemcitabine NULL NULL Quantitative real-time PCR pancreatic cancer (gemcitabine-sensitive) BxPC-3 cells and gemcitabine-resistant MIAPaCa-E (relatively resistant to gemcitabine) and MIAPaCa-M (highly resistant to gemcitabine) 20388782 2010 up-regulated
miR-200c 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 up-regulated
miR-200c 1,3-bis(2 chloroethyl)-1-nitrosourea (BCNU) NULL 2578 Quantitative real-time PCR Human umbilical vein endothelial cells 21527937 2011 up-regulated
miR-200c Hydrogen peroxide (H2O2) NULL 784 Quantitative real-time PCR Human umbilical vein endothelial cells 21527937 2011 up-regulated
miR-200c DNA methyltransferases (DNMTs) + 5-azacytidine (5-AzaC) NULL NULL Quantitative real-time PCR umbilical cord blood-derived multipotent stem cells(hUCB-MSCs) 21572997 2011 up-regulated
miR-200c Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-200c Metformin approved 4091 Quantitative real-time PCR pancreatic cancer cells 22086681 2012 up-regulated
miR-200c Metformin approved 4091 Quantitative real-time PCR pancreatic cancer cells 22086681 2012 up-regulated
miR-200c CDF(analogues of curcumin) NULL NULL Quantitative real-time PCR pancreatic cancer cells 22108826 2012 up-regulated
miR-200c Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 up-regulated
miR-200c Paclitaxel + Cyclopamine NULL NULL Quantitative real-time PCR prostate cancer cell 22768203 2012 up-regulated
miR-200c Catechin approved 9064 Microarray apoE−/− mice 22253797 2012 up-regulated
miR-200c Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-200c Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 up-regulated
miR-200c Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated
miR-200c Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 down-regulated
miR-200c Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 up-regulated
miR-200c Curcumin NULL 969516 Quantitative real-time PCR Y79 RB cells. 22510010 2012 up-regulated
miR-200c Furan NULL 8029 Quantitative real-time PCR liver 22079235 2011 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-200c-3p Paclitaxel 36314 NSC125973 approved sensitive Low Ovarian Cancer tissue and cell line (HeyTGL)
mmu-miR-200c-3p Docetaxel 148124 NSC628503 approved sensitive Low Gastric Cancer tissue and cell line (BGC-823)
mmu-miR-200c-3p Erlotinib 176870 NSC718781 approved resistant Low Non-Small Cell Lung Cancer tissue and cell line (PC-9, ErlR)
mmu-miR-200c-3p Trastuzumab sensitive Low Breast Cancer tissue and cell line (SKBR3)

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