miRTarBase - #MIRT007042


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pre-miRNA Information
pre-miRNA mmu-mir-511   
Genomic Coordinates chr2: 14261003 - 14261081
Synonyms Mirn511, mmu-mir-511, Mir511
Description Mus musculus miR-511 stem-loop
Comment This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-511-3p
Sequence 49| AAUGUGUAGCAAAAGACAGGAU |70
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Rock2   
Synonyms B230113H15Rik, ROKalpha, Rho-kinase, Rock-II, Rock2m, mKIAA0619
Description Rho-associated coiled-coil containing protein kinase 2
Transcript NM_009072   
Expression
Putative miRNA Targets on Rock2
3'UTR of Rock2
(miRNA target sites are highlighted)		 
>Rock2|NM_009072|3'UTR
   1 CTGCCTTCTATGAAAGCAGTTATTGAAGGTGATCGTATTCTTCAAGCTACAAGACTGAAATAAGATTTCTCAAAACTAAA
  81 AGGCTATATTTTACTGAGACACATACATACACCACACGTGCGCGCGCATGCTTGCCCTACCTTCCCCCCTGAAATAATTA
 161 CAAATTATGAAGAGGTTTTTTCTGGGCTACTTTGAAACAAGTTGAACAAAAGTGGTCGGTGAGTAAAGGTGTCCTGCAGT
 241 TCTCCCAGACTGCGTCATAAAGTCACACTACATTGCACAGGACTGAGAAGAGTTTAAAGAAATCACATTTCAGCTTCAAC
 321 AGAGGGTTTCACCAGCACATTAGCCAGAAGAATTTGGGAGTGAATCCCACTACAGCGATGCTGACTGCATCTTTAAGAAG
 401 TGACCATGATATTGTGTTCATGTATGTATATACATATGAGTTGAGCCTCATATAGATATGTACACACATACACATAAAAC
 481 ACATTGTACTGTAATACTGCAAGGTTTTTTTTAACTTACCATTTTATTTTTTATACACATTAATCAGCTATCATTATTTC
 561 AGTTGCAACTATGCACTTGTATAAAGCCATAATGTTGGAGTTTATATCCTTCATTCATGTACCTAATAAGGAGTTGCATG
 641 TTTGTGTAAAAAAAAAAATAACTGTAACAAGAAGTTTGAAGTTAATTATTCCAGTTTCCTAATGCTTAAGGTAGGCAGCT
 721 TTACCCATTTTGAATGCCTTAATTTAATTTTCTCTCCCCCCAAATCTCAGCCCTTTTCTATATTTAACTAAAGAAAGAAC
 801 AAGTGTTTACCAGCTCTTAGGATGCAGCTTGCTTTGTGGGGAAAGTAGTGCACAATTTTATGTGTAGTAGTTATATCAGT
 881 GTTGGCCTATTTGATGTATATTGGTTTTTAATGTACTTGGTCATAGAAACAATAATGGATTCTATTGAAACTAATGTGTG
 961 TGATGTACCTCTGTTACTGTTTTCATCCCTTTTTGCAGACTTTAGCAGGTCTGTGACTATAGAATATTTCCTTTGCTTTG
1041 AACTTTCTGGTTACCTTAGATGTGTTTTTATTTATGGTAAAGATAATTTTTCATGTTTTCATTCAATATTCAAAAATTGT
1121 CTCAAATATAAGGAAAACTAACATGTACTGTGTAGATATATTTTAAATATTTAAATGTGATCCTAAAAAAAAATCTGTGT
1201 GTGGCAATATTTCAGCTTTGATATCTGGGAAGAGGCAGCTTCAAAAGTGCTTAATTTGATTTTCACTGTTAAATGCTGCT
1281 GAGGGTAGAGAACAGGTAGGACCTATCCTGCCATGCTACTGGCCCAGGTAAGTGTGCTTAACCCCACAGCAGAGTGCAGC
1361 AGTGAGCAGCATCAGGCTCTGTACATATGCAAAGTTCAATATGACATTGAAATGTTTGCCTGATATTTTAGTGAAACCAG
1441 TAGACATTTTTGATTGCTTCCTTCTAGGGTCAGTGTAGAGATGTTGTAAAAACTTTGATCCTCGCTATAGTTACTATTGT
1521 GGAAATAGTGGCTGTTGGCCCACTGATATACTAAATCCAGTTCTGTGACCTTCAGATGGCCCAGTTTGCATCTTTCATGC
1601 TTTCTGTTAAGATTACATGTGCATGCCATTTCCCTGGAAGCAGCTGTGCTCTGGTTGGGAACACAACTTGCTTCCTCCCT
1681 CTTCTCCCTCCCTCCTTCCTTCAGGTTTAAAAATGAAAATATATAAAATTTATATAGAAGAACTATTTCCATTCATTAGA
1761 GTTGTTTAAAAGGAGACTGAATTAATATTTTATATAAAGATTTTGTTACTATGGGAGGTTCTATCCTATTCTGAATTGGA
1841 GAGTATATATATATATATATTTTTAATAAACTTTATTAAGGTGAAATAATGTCAAGTTTACACATGAATAATTGAAGTAT
1921 TTGAGTAAAAAAGGAAAAAGTTTTAATTTTGGATGCTGTTCGTGTATATATGTGTAAGAGTTTGGAGAATCATGATGTGA
2001 TACAACTGTATAAGGCAATTAATGTGAAGGATTCAGGGGAATAAAACATCCTGAGGGGTGAAAAAAAAATCTCTCCATGG
2081 AGCGTCTTCACATATGGCTTACTAGGGATTTATAAGGTTATTTGTCCAACAGTCAGAATTCCAAGTAAAAGCCACACGAC
2161 CTTAGAGAAGCATTCACTCTTCGCACTCATCTCCATGTATGTGGGTGCGCATGCCTGCCATTTCCTCCCCAAACCAACAG
2241 TCATTACAACAACAACCTAATTTTGTGCCAGTTTCTTTTTTTCTTGAGGCCTCCAATCCTTCAAGATAAATGTGTACCAG
2321 CACAAATACTGCTTCCTTTGGTCCCCCAGAGCTGTAAATACTTGGCATTTGGGATCTAAAAGACAGGGAATGCTGGGTTT
2401 CCTTAGTTGGATGCTATTCTTATCCTTTGTGGAAATAAAAAAGACAGACGTAAAAGAAAAACTTAAGTACCAGTATTACT
2481 GTTGAAAATTAATGTAGAATTAATTTTTAAGCGTTAATTTTCAGTAACATCTCAAAGCAAGATTAAAACAAAACAAAACT
2561 GTGATCCCAAGGGAAGGCTGCTGTTTGTGGCCAACCTTTGAGAGGCCATGGCCCTAGAGGGCTTTGCTCAGTTCATGTGT
2641 AGATCCTAGTGAGACATGCTCGAGGCTGCCAAACCAACTGAAGATGGCATACTGCTGCTGTCAGTCTGTTAGGGTTCCCA
2721 GGGTGAAGGAAGAGAAACTCAGACACTAACTAACCTTGTAGAGATGCGTTAGGCAGCATGGTGAAGCCCCCAGTAAAGCA
2801 CGTAGCTTCCGAGCTTCTCAGGCCCTTGACTGCTACTGATTCTTTTTTTTCTGACTGAAGATGACTTCAGCTCCCCTGGC
2881 TCGTGGAGGTGCCACAGCCAATGTTAAAGCACTTTGACAAGCACTGTGTGACAGTGCTGAGTGTTGTTCCCTGGGACCTC
2961 ACCTTTGTTCCTTTCCCTGTGGTGACTTTTGTTTGCAACTGTAATAAGTTATAAACTGCATGATTTAGAGAGTCATCTTG
3041 GATCAGTCCCTTCCCCCAACAAAAAAATCATCCCCTCTTGATTTATTTTTTATTTTGTTGCATCTTTGTAGTAATGTAAT
3121 TGTATTTTTATGCCTGCTTTTTGTTTAAAAAAATAAATAAAAGAAACTAAGATGTAAGCATCTTTTCCTGTGAAATAGTT
3201 GTGGAATGCTGCAGCTTCTTCACCTGTCTTCTTAAAGGATGCTAATAGTAGATGAGGTGATGTCATCATAGTGAAATCAC
3281 TTCTGCCTTACATCCTGGAGAGTTTAGTTCTAGAGTCCGCCTCCGATTCACTTTGGGTATGCAGGCTTAAACATCTGATT
3361 CCAGGTGGAAAGGGTAGAGGGCAGAGAGTGCCTTTCTGTCTGAAAACAGTATGATGGACACTTCCTAGAAGGTGTCTTAC
3441 TGGGAATTTATGGCTGCTTTTTGTTCTGGGGTTGGGTATTCTGTAAACTGGAGACTGTCTACTTGTTGGTTTGAAATACG
3521 ATAATAGTGCATGTGAGATAGCTGTAGTAACTCAGATATTCCTTCCAAAAGTGGGGAAATGGCCAGTGATGGGGATGCAT
3601 GCTGGTAAAATACATTGAAGAGATGAAGGCAGA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' uaGGACAGAAAACGAUGUGUAa 5'
            ::| |:||||  ||||||| 
Target 5' atTTTATTTTTT-ATACACATt 3'
 521 - 541 159.00 -9.20
2 
miRNA  3' uaggACAGAAAACGAUGUGUAa 5'
              ||||   | :||||||| 
Target 5' ataaTGTCAAGT-TTACACATg 3'
 1886 - 1906 149.00 -9.60
3 
miRNA  3' uaggacagaaaacgAUGUGUAa 5'
                        ||||||| 
Target 5' gatatgtacacacaTACACATa 3'
 455 - 476 140.00 -7.74
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions FLSs , HEK 293T
Disease 2013/5/10 23:40
Location of target site 3'UTR
Tools used in this research DAVID , DIANA-microT , TargetScan
Original Description (Extracted from the article) ... "We then performed dual-luciferase assays to test the 3 0 UTRs of a small panel of miR-511-3p predicted targets ...

- Squadrito ML; Pucci F; Magri L; Moi D; et al., 2012, Cell reports.

Article - Squadrito ML; Pucci F; Magri L; Moi D; et al.
- Cell reports, 2012
Expression of the mannose receptor (MRC1/CD206) identifies macrophage subtypes, such as alternatively activated macrophages (AAMs) and M2-polarized tumor-associated macrophages (TAMs), which are endowed with tissue-remodeling, proangiogenic, and protumoral activity. However, the significance of MRC1 expression for TAM's protumoral activity is unclear. Here, we describe and characterize miR-511-3p, an intronic microRNA (miRNA) encoded by both mouse and human MRC1 genes. By using sensitive miRNA reporter vectors, we demonstrate robust expression and bioactivity of miR-511-3p in MRC1(+) AAMs and TAMs. Unexpectedly, enforced expression of miR-511-3p tuned down the protumoral gene signature of MRC1(+) TAMs and inhibited tumor growth. Our findings suggest that transcriptional activation of Mrc1 in TAMs evokes a genetic program orchestrated by miR-511-3p, which limits rather than enhances their protumoral functions. Besides uncovering a role for MRC1 as gatekeeper of TAM's protumoral genetic programs, these observations suggest that endogenous miRNAs may operate to establish thresholds for inflammatory cell activation in tumors.
LinkOut: [PMID: 22832163]
131 mmu-miR-511-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT007042 Rock2 Rho-associated coiled-coil containing protein kinase 2 1 1
MIRT410979 Ado 2-aminoethanethiol (cysteamine) dioxygenase 2 2
MIRT577699 Slc28a3 solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 2 2
MIRT577858 Rassf5 Ras association (RalGDS/AF-6) domain family member 5 2 2
MIRT578125 Nqo2 N-ribosyldihydronicotinamide quinone reductase 2 2 2
MIRT578178 Neu1 neuraminidase 1 2 2
MIRT578289 Mavs mitochondrial antiviral signaling protein 2 2
MIRT578542 Htr1f 5-hydroxytryptamine (serotonin) receptor 1F 2 2
MIRT578630 Gtf2h2 general transcription factor II H, polypeptide 2 2 2
MIRT578655 Gramd1c GRAM domain containing 1C 2 2
MIRT578681 Golt1a golgi transport 1A 2 2
MIRT578805 Folh1 folate hydrolase 1 2 2
MIRT578909 Entpd1 ectonucleoside triphosphate diphosphohydrolase 1 2 2
MIRT579078 Cox15 cytochrome c oxidase assembly protein 15 2 4
MIRT579092 Cnih3 cornichon family AMPA receptor auxiliary protein 3 2 4
MIRT579359 Aplnr apelin receptor 2 2
MIRT579390 Alkbh1 alkB homolog 1, histone H2A dioxygenase 2 6
MIRT579984 Wnt7a wingless-type MMTV integration site family, member 7A 2 2
MIRT580016 Whsc1l1 nuclear receptor binding SET domain protein 3 2 4
MIRT580121 Ubn2 ubinuclein 2 2 2
MIRT580264 Trim12c tripartite motif-containing 12C 2 2
MIRT580360 Tmem26 transmembrane protein 26 2 2
MIRT580400 Tmem170b transmembrane protein 170B 2 2
MIRT580449 Tln2 talin 2 2 2
MIRT580833 Smarca2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 2 2
MIRT580872 Slc7a11 solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 2 2
MIRT581115 Sept3 septin 3 2 2
MIRT581330 Rgs8 regulator of G-protein signaling 8 2 2
MIRT581475 Rabgap1 RAB GTPase activating protein 1 2 2
MIRT581599 Prkcd protein kinase C, delta 2 2
MIRT581810 Plag1 pleiomorphic adenoma gene 1 2 2
MIRT581916 Pgm3 phosphoglucomutase 3 2 2
MIRT582067 Onecut2 one cut domain, family member 2 2 2
MIRT582557 Mal2 mal, T cell differentiation protein 2 2 2
MIRT582599 Lrrc40 leucine rich repeat containing 40 2 2
MIRT582756 Klf8 Kruppel-like factor 8 2 2
MIRT582969 Igf2 insulin-like growth factor 2 2 4
MIRT583021 Htt huntingtin 2 2
MIRT583256 Gnb4 guanine nucleotide binding protein (G protein), beta 4 2 4
MIRT583615 Fam46a family with sequence similarity 46, member A 2 2
MIRT583904 Drp2 dystrophin related protein 2 2 2
MIRT584439 Ccdc85a coiled-coil domain containing 85A 2 2
MIRT584855 Appl1 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 2 2
MIRT585490 Txlnb taxilin beta 2 2
MIRT585697 Tbc1d24 TBC1 domain family, member 24 2 2
MIRT585737 Steap2 six transmembrane epithelial antigen of prostate 2 2 2
MIRT585876 Slc22a8 solute carrier family 22 (organic anion transporter), member 8 2 8
MIRT585961 Sike1 suppressor of IKBKE 1 2 6
MIRT586179 Ptprr protein tyrosine phosphatase, receptor type, R 2 2
MIRT586592 Mrgpre MAS-related GPR, member E 2 2
MIRT586808 Ints8 integrator complex subunit 8 2 2
MIRT586916 Heatr2 dynein, axonemal assembly factor 5 2 4
MIRT586949 Gstt3 glutathione S-transferase, theta 3 2 2
MIRT587148 Gcnt4 glucosaminyl (N-acetyl) transferase 4, core 2 (beta-1,6-N-acetylglucosaminyltransferase) 2 2
MIRT587226 Nxpe3 neurexophilin and PC-esterase domain family, member 3 1 1
MIRT587477 D630045J12Rik RIKEN cDNA D630045J12 gene 2 8
MIRT587487 D630003M21Rik RIKEN cDNA D630003M21 gene 2 2
MIRT587601 Cml2 N-acetyltransferase 8 (GCN5-related) family member 2 2 2
MIRT587746 Cd28 CD28 antigen 2 2
MIRT587777 Ccpg1 cell cycle progression 1 2 2
MIRT587838 Casp8 caspase 8 2 4
MIRT588179 A530054K11Rik zinc finger protein 729a 2 2
MIRT588286 1700019G17Rik N-acetyltransferase 8 (GCN5-related) family member 4 2 2
MIRT588363 Zfp518b zinc finger protein 518B 2 2
MIRT588390 Zeb2 zinc finger E-box binding homeobox 2 2 2
MIRT588545 Uhrf1bp1l UHRF1 (ICBP90) binding protein 1-like 2 2
MIRT588613 Tsc22d3 TSC22 domain family, member 3 2 2
MIRT588648 Tmem200a transmembrane protein 200A 2 2
MIRT588722 Tbx22 T-box 22 2 2
MIRT589666 Lcorl ligand dependent nuclear receptor corepressor-like 2 2
MIRT589865 Hecw1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 2 2
MIRT589974 Gabpb2 GA repeat binding protein, beta 2 2 2
MIRT590191 Ell2 elongation factor RNA polymerase II 2 2 2
MIRT590365 Clca2 chloride channel accessory 3A2 2 2
MIRT590839 Wdr46 WD repeat domain 46 2 2
MIRT590855 Vps33b vacuolar protein sorting 33B 2 4
MIRT590931 Supt7l suppressor of Ty 7-like 2 2
MIRT591032 Rfx3 regulatory factor X, 3 (influences HLA class II expression) 2 2
MIRT591224 Ly96 lymphocyte antigen 96 2 2
MIRT591305 Il18r1 interleukin 18 receptor 1 2 2
MIRT591362 Egfl6 EGF-like-domain, multiple 6 2 2
MIRT591431 Car10 carbonic anhydrase 10 2 4
MIRT591524 Abcc9 ATP-binding cassette, sub-family C (CFTR/MRP), member 9 2 2
MIRT591574 Zfhx3 zinc finger homeobox 3 2 4
MIRT591596 Xrcc3 X-ray repair complementing defective repair in Chinese hamster cells 3 2 2
MIRT591612 Vps37a vacuolar protein sorting 37A 2 2
MIRT591706 Rpusd2 RNA pseudouridylate synthase domain containing 2 2 2
MIRT591719 Rorb RAR-related orphan receptor beta 2 4
MIRT591738 Rab9b RAB9B, member RAS oncogene family 2 4
MIRT591833 Lin7a lin-7 homolog A (C. elegans) 2 2
MIRT591845 Iglon5 IgLON family member 5 2 2
MIRT591896 Fbrs fibrosin 2 2
MIRT591953 Ceacam1 carcinoembryonic antigen-related cell adhesion molecule 1 2 2
MIRT592281 Gatc glutamyl-tRNA(Gln) amidotransferase, subunit C 2 2
MIRT592403 Tmco1 transmembrane and coiled-coil domains 1 2 2
MIRT592457 Ski ski sarcoma viral oncogene homolog (avian) 2 2
MIRT592464 Runx1 runt related transcription factor 1 2 2
MIRT592652 Lifr leukemia inhibitory factor receptor 2 2
MIRT592681 Has2 hyaluronan synthase 2 2 2
MIRT592828 Asb7 ankyrin repeat and SOCS box-containing 7 2 2
MIRT592841 Akap2 A kinase (PRKA) anchor protein 2 2 2
MIRT593386 Adra1b adrenergic receptor, alpha 1b 2 2
MIRT593398 Znrf3 zinc and ring finger 3 2 2
MIRT593410 Slc6a6 solute carrier family 6 (neurotransmitter transporter, taurine), member 6 2 2
MIRT594175 Vdr vitamin D (1,25-dihydroxyvitamin D3) receptor 2 2
MIRT594720 Zfp931 zinc finger protein 931 2 2
MIRT594897 Ifi204 interferon activated gene 204 2 2
MIRT594917 Gm14326 predicted gene 14326 2 2
MIRT594951 Fblim1 filamin binding LIM protein 1 2 2
MIRT595342 Frmd4a FERM domain containing 4A 2 2
MIRT595370 Fam168b family with sequence similarity 168, member B 2 2
MIRT595396 Bcl10 B cell leukemia/lymphoma 10 2 2
MIRT595401 Arsk arylsulfatase K 2 2
MIRT595424 Adamts12 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12 2 2
MIRT595429 A630033H20Rik RIKEN cDNA A630033H20 gene 2 2
MIRT595440 9030617O03Rik D-glutamate cyclase 2 2
MIRT595727 B2m beta-2 microglobulin 2 2
MIRT601791 Rab27a RAB27A, member RAS oncogene family 2 2
MIRT602942 Xpo7 exportin 7 2 2
MIRT604983 Fsd1l fibronectin type III and SPRY domain containing 1-like 2 2
MIRT605311 Zfp92 zinc finger protein 92 2 2
MIRT605455 St18 suppression of tumorigenicity 18 2 2
MIRT605533 Pstpip2 proline-serine-threonine phosphatase-interacting protein 2 2 2
MIRT605596 Nbeal1 neurobeachin like 1 2 2
MIRT605765 Epas1 endothelial PAS domain protein 1 2 2
MIRT605940 Sema6a sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A 2 2
MIRT605982 Mylk4 myosin light chain kinase family, member 4 2 2
MIRT606186 Slc5a8 solute carrier family 5 (iodide transporter), member 8 2 2
MIRT606235 Rab3c RAB3C, member RAS oncogene family 2 2
MIRT606619 Rbfox2 RNA binding protein, fox-1 homolog (C. elegans) 2 2 2
MIRT755997 Tlr4 toll-like receptor 4 3 1

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