ID	Duplex structure	Position	Score	MFE
1	miRNA 3' ucgGUGU-UAG---UGGAAGACUAGa 5' |||| ||: | ||||||||| Target 5' caaCACACATTGAAAGCTTCTGATCa 3'	344 - 369	163.00	-16.50
2	miRNA 3' ucgGUGUUAGUGGA-AGACUAGa 5' || |:| ||:| |||||| Target 5' tggCAGAGTGACTTAACTGATCa 3'	523 - 545	131.00	-13.90
3	miRNA 3' ucGGUGUUAG---UGGA--AGACUAGa 5' |||||:|: |||| |:| ||| Target 5' aaCCACAGTTGAAACCTGCTTTTATCa 3'	40 - 66	122.00	-13.60

Mutant ID	Mutant Position	Mutant Source
COSN15326211	35	COSMIC
COSN13256378	36	COSMIC
COSN29644532	92	COSMIC
COSN31548391	96	COSMIC
COSN31571336	127	COSMIC
COSN28705689	199	COSMIC
COSN31960563	502	COSMIC
COSN5247700	727	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs553230494	1	dbSNP
rs1488576292	5	dbSNP
rs1470965975	6	dbSNP
rs1262353738	8	dbSNP
rs1218059375	9	dbSNP
rs1007526416	14	dbSNP
rs532699212	19	dbSNP
rs891303665	20	dbSNP
rs759356949	21	dbSNP
rs1224324709	23	dbSNP
rs776477716	31	dbSNP
rs1233044467	34	dbSNP
rs770898211	41	dbSNP
rs1404648941	49	dbSNP
rs1338266889	54	dbSNP
rs1443934666	55	dbSNP
rs994294487	57	dbSNP
rs1310382641	58	dbSNP
rs1376662918	63	dbSNP
rs769063645	64	dbSNP
rs1038072808	66	dbSNP
rs1283148692	81	dbSNP
rs941048193	89	dbSNP
rs1200013769	103	dbSNP
rs1276598683	104	dbSNP
rs926921752	109	dbSNP
rs1044326879	118	dbSNP
rs887167675	121	dbSNP
rs761949422	127	dbSNP
rs949967312	133	dbSNP
rs376898915	135	dbSNP
rs1238519817	142	dbSNP
rs17098493	155	dbSNP
rs1438898811	164	dbSNP
rs1176684627	172	dbSNP
rs1309789477	175	dbSNP
rs1396883234	180	dbSNP
rs917169325	183	dbSNP
rs988675254	185	dbSNP
rs1399458614	187	dbSNP
rs955842629	192	dbSNP
rs7768	199	dbSNP
rs142324456	214	dbSNP
rs530292194	221	dbSNP
rs1445109970	227	dbSNP
rs1040400911	228	dbSNP
rs1366031394	238	dbSNP
rs1233176621	239	dbSNP
rs968055856	240	dbSNP
rs1341600247	241	dbSNP
rs775187590	252	dbSNP
rs1257729954	282	dbSNP
rs1018660995	286	dbSNP
rs574593732	294	dbSNP
rs955853653	304	dbSNP
rs914248831	315	dbSNP
rs1269218371	316	dbSNP
rs769459699	321	dbSNP
rs188628226	327	dbSNP
rs897312713	330	dbSNP
rs924384402	335	dbSNP
rs1038097296	341	dbSNP
rs1170937253	344	dbSNP
rs1005224461	350	dbSNP
rs981206128	351	dbSNP
rs905529787	366	dbSNP
rs1044492316	368	dbSNP
rs971677105	370	dbSNP
rs745342525	371	dbSNP
rs1391933456	381	dbSNP
rs991821649	384	dbSNP
rs949737301	386	dbSNP
rs1328255548	396	dbSNP
rs527936323	404	dbSNP
rs1053004928	410	dbSNP
rs780693514	413	dbSNP
rs1004190895	415	dbSNP
rs1235394832	437	dbSNP
rs3377	448	dbSNP
rs1262812168	449	dbSNP
rs746960344	453	dbSNP
rs998280990	454	dbSNP
rs946754731	458	dbSNP
rs1261856891	459	dbSNP
rs911308509	459	dbSNP
rs183853491	464	dbSNP
rs575769568	468	dbSNP
rs1476608470	471	dbSNP
rs955389738	475	dbSNP
rs1030352673	496	dbSNP
rs972930064	500	dbSNP
rs894040191	506	dbSNP
rs1161970262	507	dbSNP
rs1387688734	515	dbSNP
rs1401157074	523	dbSNP
rs961531572	543	dbSNP
rs1454106809	546	dbSNP
rs1297603240	550	dbSNP
rs1416818054	554	dbSNP
rs1017018898	556	dbSNP
rs1383160557	562	dbSNP
rs1005672477	567	dbSNP
rs562584180	569	dbSNP
rs1263464362	576	dbSNP
rs1335680626	577	dbSNP
rs1355698044	579	dbSNP
rs1217282367	584	dbSNP
rs140097493	591	dbSNP
rs1487046067	595	dbSNP
rs774265232	597	dbSNP
rs1256494185	602	dbSNP
rs35226258	605	dbSNP
rs553646574	608	dbSNP
rs1420793282	615	dbSNP
rs895457386	618	dbSNP
rs1053038624	620	dbSNP
rs949795424	626	dbSNP
rs758188478	633	dbSNP
rs1378634492	643	dbSNP
rs1394524907	649	dbSNP
rs1378386103	651	dbSNP
rs904361721	652	dbSNP
rs146047004	657	dbSNP
rs1285286618	675	dbSNP
rs943209329	681	dbSNP
rs1243761387	692	dbSNP
rs1256087143	695	dbSNP
rs1183244361	697	dbSNP
rs559047827	706	dbSNP
rs1240380934	709	dbSNP
rs1440867652	712	dbSNP
rs1178553858	713	dbSNP
rs1264344936	720	dbSNP
rs982657589	740	dbSNP
rs1176811576	742	dbSNP
rs1358678586	750	dbSNP
rs1464698416	752	dbSNP
rs911325484	756	dbSNP
rs951425892	759	dbSNP
rs1485860326	760	dbSNP

- Brain pathology (Zurich, Switzerland), 2013

Accumulating evidence suggests that microRNAs (miRNAs) are over- or under-expressed in tumors, and abnormalities in miRNA expression may contribute to carcinogenesis. MiR-383 was previously identified as one of the under-expressed miRNAs in medulloblastoma (MB) by miRNA expression profiling. Quantitative reverse transcription polymerase chain reaction (RT-PCR)-based miRNA assays showed an enrichment of miR-383 in normal brain. Based on these data, we speculated that miR-383 is important in MB pathogenesis. In this study, we demonstrated significant downregulation of miR-383 in 23/29 (79%) MB samples and 7/7 (100%) MB cells lines. Ectopic expression of miR-383 in MB cells led to suppression of cell growth, cell accumulation at sub-G1 phase and alteration of apoptosis-related proteins. By transcriptomic analysis and computational algorithms, we identified peroxiredoxin 3 (PRDX3) as a target gene of miR-383. Luciferase reporter assay confirmed that miR-383 negatively regulated PRDX3 by interaction between miR-383 and complementary sequences in the 3' UTR of PRDX3. MiR-383 repressed PRDX3 at transcriptional and translational levels as revealed by quantitative RT-PCR and Western blot analysis. Furthermore, depletion of PRDX3 by siRNAs resulted in similar effects as observed in miR-383-transfected cells. In conclusion, miR-383 acts as a regulator controlling cell growth of MB, at least in part, through targeting PRDX3.