miRTarBase - #MIRT010922


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pre-miRNA Information
pre-miRNA mmu-mir-324   
Genomic Coordinates chr11: 70012043 - 70012131
Synonyms Mirn324, mir-324, mmu-mir-324, Mir324
Description Mus musculus miR-324 stem-loop
Comment Kim et al. cloned 40 new miRNAs from rat E18 primary cortical neurons .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-324-3p
Sequence 53| CCACUGCCCCAGGUGCUGCU |72
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Tmem135   
Synonyms 2810439K08Rik, AW319712, PMP52
Description transmembrane protein 135
Transcript NM_028343   
Expression
Putative miRNA Targets on Tmem135
3'UTR of Tmem135
(miRNA target sites are highlighted)		 
>Tmem135|NM_028343|3'UTR
   1 AGATGACTGTAACTTATTGACTGTGTCTCACCATTTTCTCCTGAAGAGTTAATTATGTTCAACACAGAAGGGGCCCAAGC
  81 TGAACCTCAGTGTTACGTCAACGAGAGATGCTTTTTCTTTCTTTTCATACCAATCAGAAATACAGAAGCTTTTTAGAAAG
 161 GCGTTGCTTAATAATTAAGCTTCCTCTGTAGCCAGAATCTCATTCTGGATCATGTAGTGTTGACATTATGATATATTGTT
 241 GATTAAATTATGTCCACAAAGAATATTGAATAATCTATGTAGAAATATAATAAGAAGAGTATACTTAAAAATTACTTTAA
 321 AAGATATCTTTAGTTCATTCCAATAGAATTCTTGGTCAAAACTAAGAATATTTCTTCACTTTAGGATTTGCAAAGGATCT
 401 CGGGTACATGGATTCAGCCCGTGATCTTTACGCTTTTGAATGGATTTCTGTAGCTGTGGGGCGTTGGAGATGGGGCATTG
 481 CTGCTTTGCTGTGGAGCAATGTGGAGAAAGTGGCCATTCTTATTTCAGGGATACAAACGTGGGTTTCATACCATTTGAGC
 561 CAGTGTCATGGTGGTACCCATACTCTTTTGTTCAGCTCTCAGTCCTGTCTCTCTTCGAGACCAGATGGGCTGGTTGGCAG
 641 CCTAGTGACCTTATATGATAATAGCAGGTTAGGAACAAATGGAAACCGACAGGTGCTGCTGTAAAAAATGTCTCAGTGTT
 721 CACAGGAGTGTTACACTTGAATATTGTCAAGCAGACAACCTAAAAGCCAATCTTCACACCTCCTATAGGCAGATTGTGTT
 801 TATTTTCACCACAGTGGCTGTGCTCTTTATAGCTCAGTTTCAATGTCATTGGTGTTTTAGAAAGGGTTAAGATCCTATGG
 881 AACAAAAGAGCTTTGGGCCAGTTTGACAGTTGGTACCCCCTCTAATGCCTCACCTAAATGGGGCGTTTTATGACGAAAAA
 961 TGTGAAAATGCAGTGATTGTTGGGAGTTCCTCTCTCACTAATTGGCTTCCTGTTCATTAACCAGTTCTGGCTGGAGTTAA
1041 GGGACACCAGATGGTTGGTGCTGATTATAGAAGCAGTCCTGGACAGCTGACTGAGATATGATCACTTGGCTTTTCTGGGG
1121 AAAGTTGTACTTTTCTAAGTTAATATAGAAGTCTAAAGTGGCTGTGATGTCTACAGTTAATGTAATATATAGGAATTATC
1201 TTTAAATATATAATTTTGTTGCTAAAAATGTTTCAGGCCATTGTAAGTTATGCAGTAGAACTTGTGTTATGACAGGAATT
1281 GTAACTCAAGACATTAAAAATACTTAACCTCCCTTATTCAGTTGCATCAGCACTTGTATTTCTGTGTAACCCTACCCTTG
1361 GTTGTTATAGAAAATTGAGTCCCTACAAAGAGACAGACTCTAGTGCCATCATGTAACAGAACCACTTGAGTTTGCTCAGA
1441 ATTTTGCGTTACGATTAGCGTTCTAGAGTGGACTTACCTGCTTGGTAACACATTGAAGGGTCATTTTGGGTTTGGACTTC
1521 CACACTGATTAATTAATTAATGCAGTTTTAGGTTTTTATTCACTGGAATCGGACTGATGATTAGTACTGTGTTTCCCTTT
1601 GAAAATGAAGTATTAAAGCACTGAGAACGCCTGGCTACCTGGACCTCATTTTCTCCTCTCAGGTTTTATGTCTGTTGTGT
1681 TTTTCTTGTCAGGTTAAAAAATCTATTTTACGCCATAAGTCATTCTTTTCATTGCCATCAAAAGACTGTGATAATGATTA
1761 ATCCTATCGGAATGTGCTAAAAATGTTTAGTAAAATCTTGCTGGTCAAAAAGGTGCATTTCTGTGAGAGATATTTTTAAC
1841 GAATTACACTCGTGTTTTTATTTTATGAAAAATGTAATAAGCAACAGCTACCATGAAAGAGAACATAGCAGTGACTGTCC
1921 AGCTCAGAAGGGTAGCGCACGGACATGCAGTGACGACCGAGAAATACAGTTTGAACTCTTGAGGACAGTGTGCTTGTTCA
2001 AGCAAACAAGAAGAAAACTTGGCGTTTCTAAAGACAATCTGTGTCTTTCTACCGCTAACTAATCAGGAATTGACTGACAG
2081 CTCCCTGTCATCATAAATAAAGGCAAATAGATCTCTC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ucGUCGUGGACCCCGUCACc 5'
            :| ||::|| | ||||| 
Target 5' caTACCATTTGAGCCAGTGt 3'
 547 - 566 130.00 -10.90
2 
miRNA  3' ucgUCGUGGAC-CCCGUCACc 5'
             | | |:|| || ||||| 
Target 5' ttgAACTCTTGAGGACAGTGt 3'
 1971 - 1991 125.00 -13.00
3 
miRNA  3' ucguCGUGGACCCCGUCACc 5'
              | |  ||||||| || 
Target 5' cgttGGAGATGGGGCATTGc 3'
 462 - 481 124.00 -14.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
CLIP-seq Support 1 for dataset GSM4656410
Method / RBP HITS-CLIP / AGO
Cell line / Condition mouse liver / e185 rep2
Location of target site NM_028343 | 3UTR | UGAAAAUGCAGUGAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE153876
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4751757
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 2
Location of target site NM_028343 | 3UTR | UGACUGUCCAGCUCA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM4751758
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 3
Location of target site NM_028343 | 3UTR | UGACUGUCCAGCUCA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM4751760
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 1
Location of target site NM_028343 | 3UTR | UGACUGUCCAGCUCA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
351 mmu-miR-324-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT010726 March8 membrane-associated ring finger (C3HC4) 8 1 1
MIRT010727 Lix1 limb and CNS expressed 1 1 1
MIRT010728 Cplx1 complexin 1 1 1
MIRT010729 Mapk6 mitogen-activated protein kinase 6 1 1
MIRT010730 Fam19a5 family with sequence similarity 19, member A5 1 1
MIRT010731 Myo6 myosin VI 1 1
MIRT010732 Galnt7 UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 7 1 1
MIRT010733 Fam195b MAPK regulated corepressor interacting protein 1 1 1
MIRT010734 Syp synaptophysin 1 1
MIRT010735 Gspt2 G1 to S phase transition 2 1 1
MIRT010736 Gpam glycerol-3-phosphate acyltransferase, mitochondrial 1 1
MIRT010737 Tln1 talin 1 1 1
MIRT010738 Gja1 gap junction protein, alpha 1 1 1
MIRT010739 Gprin3 GPRIN family member 3 1 1
MIRT010740 Cd99l2 CD99 antigen-like 2 1 1
MIRT010741 Lfng LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase 1 1
MIRT010742 Katnbl1 katanin p80 subunit B like 1 1 1
MIRT010743 Clock circadian locomotor output cycles kaput 1 1
MIRT010744 Garnl3 GTPase activating RANGAP domain-like 3 1 1
MIRT010745 Lsamp limbic system-associated membrane protein 1 1
MIRT010746 Zkscan8 zinc finger with KRAB and SCAN domains 8 1 1
MIRT010747 Lipa lysosomal acid lipase A 1 1
MIRT010748 Acot7 acyl-CoA thioesterase 7 1 1
MIRT010749 Stx17 syntaxin 17 1 1
MIRT010750 Smad3 SMAD family member 3 1 1
MIRT010751 Fbxo18 F-box protein 18 1 1
MIRT010752 Fam171b family with sequence similarity 171, member B 1 1
MIRT010753 Kif1b kinesin family member 1B 1 1
MIRT010754 Slc17a6 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 1 1
MIRT010755 Gga3 golgi associated, gamma adaptin ear containing, ARF binding protein 3 1 1
MIRT010756 Vat1 vesicle amine transport 1 1 1
MIRT010757 Eif1a eukaryotic translation initiation factor 1A 1 1
MIRT010758 Heg1 heart development protein with EGF-like domains 1 1 1
MIRT010759 Itm2c integral membrane protein 2C 1 1
MIRT010760 Nr2c2 nuclear receptor subfamily 2, group C, member 2 1 1
MIRT010761 Tor1aip2 torsin A interacting protein 2 1 1
MIRT010762 Rmnd5a required for meiotic nuclear division 5 homolog A 1 1
MIRT010763 Cav2 caveolin 2 1 1
MIRT010764 March6 membrane-associated ring finger (C3HC4) 6 2 2
MIRT010765 Zfp516 zinc finger protein 516 1 1
MIRT010766 4931428F04Rik RIKEN cDNA 4931428F04 gene 1 1
MIRT010767 Cln8 ceroid-lipofuscinosis, neuronal 8 1 1
MIRT010768 Ccdc50 coiled-coil domain containing 50 1 1
MIRT010769 Gpc1 glypican 1 1 1
MIRT010770 Gabra1 gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 1 1
MIRT010771 Rap2c RAP2C, member of RAS oncogene family 1 1
MIRT010772 Ttc3 tetratricopeptide repeat domain 3 1 1
MIRT010773 Rabgap1l RAB GTPase activating protein 1-like 1 1
MIRT010774 Shc4 SHC (Src homology 2 domain containing) family, member 4 1 1
MIRT010775 Canx calnexin 1 1
MIRT010776 Hyal1 hyaluronoglucosaminidase 1 1 1
MIRT010777 Acss1 acyl-CoA synthetase short-chain family member 1 1 1
MIRT010778 Zfp521 zinc finger protein 521 1 1
MIRT010779 Sytl2 synaptotagmin-like 2 1 1
MIRT010780 Tpra1 transmembrane protein, adipocyte asscociated 1 1 1
MIRT010781 Lnpep leucyl/cystinyl aminopeptidase 1 1
MIRT010782 Fem1b feminization 1 homolog b (C. elegans) 1 1
MIRT010783 Gabrb2 gamma-aminobutyric acid (GABA) A receptor, subunit beta 2 1 1
MIRT010784 Smad4 SMAD family member 4 1 1
MIRT010785 Cadm3 cell adhesion molecule 3 1 1
MIRT010786 Tm9sf4 transmembrane 9 superfamily protein member 4 1 1
MIRT010787 Tmem131 transmembrane protein 131 1 1
MIRT010788 Zeb2 zinc finger E-box binding homeobox 2 1 1
MIRT010789 Zfp414 zinc finger protein 414 1 1
MIRT010790 Pygb brain glycogen phosphorylase 1 1
MIRT010791 Gnptab N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits 1 1
MIRT010792 Prrt1 proline-rich transmembrane protein 1 1 1
MIRT010793 Jam2 junction adhesion molecule 2 1 1
MIRT010794 Ppp3r1 protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) 1 1
MIRT010795 Utrn utrophin 1 1
MIRT010796 H2-D1 histocompatibility 2, D region locus 1 1 1
MIRT010797 Lrrc4 leucine rich repeat containing 4 1 1
MIRT010798 Epb4.1l2 erythrocyte membrane protein band 4.1 like 2 1 1
MIRT010799 Patl1 protein associated with topoisomerase II homolog 1 (yeast) 1 1
MIRT010800 Ppig peptidyl-prolyl isomerase G (cyclophilin G) 1 1
MIRT010801 Evl Ena-vasodilator stimulated phosphoprotein 1 1
MIRT010802 Ralgapb Ral GTPase activating protein, beta subunit (non-catalytic) 1 1
MIRT010803 Clcn4-2 chloride channel, voltage-sensitive 4 1 1
MIRT010804 Anxa5 annexin A5 1 1
MIRT010805 Akap9 A kinase (PRKA) anchor protein (yotiao) 9 1 1
MIRT010806 Hdac6 histone deacetylase 6 1 1
MIRT010807 Nuak1 NUAK family, SNF1-like kinase, 1 1 1
MIRT010808 Rela v-rel reticuloendotheliosis viral oncogene homolog A (avian) 1 1
MIRT010809 Impad1 inositol monophosphatase domain containing 1 1 1
MIRT010810 Ncan neurocan 1 1
MIRT010811 Usp6nl USP6 N-terminal like 1 1
MIRT010812 2700081O15Rik RIKEN cDNA 2700081O15 gene 1 1
MIRT010813 Slc24a2 solute carrier family 24 (sodium/potassium/calcium exchanger), member 2 1 1
MIRT010814 Mbip MAP3K12 binding inhibitory protein 1 1 1
MIRT010815 Bag4 BCL2-associated athanogene 4 1 1
MIRT010816 Eps15 epidermal growth factor receptor pathway substrate 15 1 1
MIRT010817 Gnao1 guanine nucleotide binding protein, alpha O 1 1
MIRT010818 Ncam1 neural cell adhesion molecule 1 1 1
MIRT010819 Bola2 bolA-like 2 (E. coli) 1 1
MIRT010820 Lin7c lin-7 homolog C (C. elegans) 1 1
MIRT010821 Whsc1l1 nuclear receptor binding SET domain protein 3 1 1
MIRT010822 Enpp4 ectonucleotide pyrophosphatase/phosphodiesterase 4 1 1
MIRT010823 Slc4a8 solute carrier family 4 (anion exchanger), member 8 1 1
MIRT010824 Pptc7 PTC7 protein phosphatase homolog 1 1
MIRT010825 Fbxl18 F-box and leucine-rich repeat protein 18 1 1
MIRT010826 Mtmr4 myotubularin related protein 4 1 1
MIRT010827 Chd4 chromodomain helicase DNA binding protein 4 1 1
MIRT010828 Intu inturned planar cell polarity protein 1 1
MIRT010829 Faf2 Fas associated factor family member 2 1 1
MIRT010830 Vezt vezatin, adherens junctions transmembrane protein 1 1
MIRT010831 Tacc1 transforming, acidic coiled-coil containing protein 1 1 1
MIRT010832 Zzef1 zinc finger, ZZ-type with EF hand domain 1 1 1
MIRT010833 Frmd5 FERM domain containing 5 1 1
MIRT010834 Ocln occludin 1 1
MIRT010835 Dpysl2 dihydropyrimidinase-like 2 1 1
MIRT010836 Baz2a bromodomain adjacent to zinc finger domain, 2A 1 1
MIRT010837 Syt1 synaptotagmin I 1 1
MIRT010838 Reep1 receptor accessory protein 1 1 1
MIRT010839 Ankrd46 ankyrin repeat domain 46 1 1
MIRT010840 Narg2 interactor of little elongation complex ELL subunit 2 1 1
MIRT010841 C230081A13Rik pseudopodium-enriched atypical kinase 1 1 1
MIRT010842 Kif5c kinesin family member 5C 1 1
MIRT010843 Pip4k2c phosphatidylinositol-5-phosphate 4-kinase, type II, gamma 1 1
MIRT010844 Ugt8a UDP galactosyltransferase 8A 1 1
MIRT010845 Klf9 Kruppel-like factor 9 1 1
MIRT010846 Ppapdc1b phospholipid phosphatase 5 1 1
MIRT010847 Iqsec2 IQ motif and Sec7 domain 2 1 1
MIRT010848 Dvl2 dishevelled segment polarity protein 2 1 1
MIRT010849 Tmx4 thioredoxin-related transmembrane protein 4 1 1
MIRT010850 Slc31a1 solute carrier family 31, member 1 1 1
MIRT010851 Oxsr1 oxidative-stress responsive 1 1 1
MIRT010852 Snx30 sorting nexin family member 30 1 1
MIRT010853 Klhl23 kelch-like 23 1 1
MIRT010854 Zmat4 zinc finger, matrin type 4 1 1
MIRT010855 Ap2a2 adaptor-related protein complex 2, alpha 2 subunit 1 1
MIRT010856 Cbx5 chromobox 5 1 1
MIRT010857 Slc25a46 solute carrier family 25, member 46 1 1
MIRT010858 Ryk receptor-like tyrosine kinase 1 1
MIRT010859 Lmbrd1 LMBR1 domain containing 1 1 1
MIRT010860 Spred2 sprouty-related, EVH1 domain containing 2 1 1
MIRT010861 Frs2 fibroblast growth factor receptor substrate 2 1 1
MIRT010862 Acvr1b activin A receptor, type 1B 1 1
MIRT010863 Snn stannin 1 1
MIRT010864 Dgcr2 DiGeorge syndrome critical region gene 2 1 1
MIRT010865 Col12a1 collagen, type XII, alpha 1 1 1
MIRT010866 Fam104a family with sequence similarity 104, member A 1 1
MIRT010867 Cbfb core binding factor beta 1 1
MIRT010868 Sdc2 syndecan 2 1 1
MIRT010869 Cdc27 cell division cycle 27 1 1
MIRT010870 G3bp2 GTPase activating protein (SH3 domain) binding protein 2 1 1
MIRT010871 Riok3 RIO kinase 3 1 1
MIRT010872 Osbpl9 oxysterol binding protein-like 9 1 1
MIRT010873 Prkcb protein kinase C, beta 1 1
MIRT010874 Chrna4 cholinergic receptor, nicotinic, alpha polypeptide 4 1 1
MIRT010875 Padi2 peptidyl arginine deiminase, type II 1 1
MIRT010876 Cd200 CD200 antigen 1 1
MIRT010877 Nck1 non-catalytic region of tyrosine kinase adaptor protein 1 1 1
MIRT010878 Pan3 PAN3 poly(A) specific ribonuclease subunit