miRTarBase - #MIRT053034


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pre-miRNA Information
pre-miRNA mmu-mir-7a-1   
Genomic Coordinates chr13: 58392779 - 58392886
Description Mus musculus miR-7a-1 stem-loop
Comment miR-7a (previously named miR-7) was predicted by computational methods using conservation between mouse, human and Fugu rubripes sequences . The ends of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure
pre-miRNA mmu-mir-7a-2   
Genomic Coordinates chr7: 78888277 - 78888373
Description Mus musculus miR-7a-2 stem-loop
Comment miR-7a (previously named miR-7) was predicted by computational methods using conservation between mouse, human and Fugu rubripes sequences . The ends of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-7a-5p
Sequence 19| UGGAAGACUAGUGAUUUUGUUGU |41
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Mknk2   
Synonyms 2010016G11Rik, Gprk7, Mnk2
Description MAP kinase-interacting serine/threonine kinase 2
Transcript NM_021462   
Expression
Putative miRNA Targets on Mknk2
3'UTR of Mknk2
(miRNA target sites are highlighted)		 
>Mknk2|NM_021462|3'UTR
   1 CCCCCACTAGCTCCTTGTACATATGCCCCTGCCCCGCGGGGCCTGAAGGCTAGGGACCTGGACACCCCACCCCTTGCCAT
  81 TCCAGGTGCCAGCTCAGCTGGGTCCTCTGGGGGTGTAGGGGTCTGTTAGGGGGTGTCTCCTTTTCTCCCTGTCCTTCCCC
 161 TGCCCTGCCCACTTGGCTTTGTTTTGTTTGTTTTTCTTTGCCGCTATTGAAAGCAAGTGCCCGGAGGAGGGCGGAGGGCT
 241 CAGGCCGCCCAGCCTGCACCCCACGATGCTCACCTGCCAACTGTGAAGGTCCTGCCACCTGCGCCCCCACCTCCACTCCA
 321 GCCACTCTGCTGTCTTCCAGGGTTGGGGATCCCGCAGGGTCAGCACCCCACACCTCTCCCAGCCCTCAGTGTTGTCAGGG
 401 ACAGGCCCTCCTGGTGAGCACAGTGGTGGTTGCATCTCCTCACCAGAGCACCCTTGGGTCTGGGGTAGGGCAGGGCTCCC
 481 TGTCTTGGATAGAGACCTCTGGGGAGCAGGTGGATGGGGACAGTGCACTTGATTGACCCCGAGTCCCCATCATCCACCTG
 561 CAGTGTCCCTTGGAGGGTTGACAATCAGAAACCCCTCCCAGGCTGCTTAGCTCCTTGCCCTGGGACAGACCTACTGCTCC
 641 CAACCCCACTTCCCAGGGGCAGAGCTGGAAGGGGACCCTGCACCCAGCTAGCTCCACCACAGCAGGAGAGGTGCTGGACC
 721 AGGCTTTCATCAGCAAACATGGGGCTCCCACATGTCTCCCCACCCAGGGCACCTGAGTGCCCCTTCTCAGGGCTCAGCCT
 801 GACCACGGCCACGTCCTGCCCCTGGGGTTCCTAAGCTCTCCTAGCTGCTTCTGTTAGCCAGAGCTGAGGCCATACCCAGG
 881 GCTCTCACCTTCCTGTTGCCCCCAGAGGGCAGCAGCTCAGGCGTGCCTGCTTTCAGGAAAGGGAGGCTGGGAAGGGATGT
 961 GGTGGCCCTGCGGTGCCCAGACCTAACTGCCCGAGGCCTGTAGACTGTTCTAGCCGAACTACTATGCAATACGAGTTCCC
1041 ATTTTCTCCATGGCCCTGCTGGTCGGGGGCGGCTGCCAGGGGCCAGGCCACCCTGCCCTGCAACTGCTCAGGTGTCTACA
1121 GGGCAGCCCCTGGCCTCAAAAATCCTTGGTCAGGATTGTTTGTCGAGTTTAGTTTAGGCTTTTTTTTTTTTTTTAAAGAA
1201 ATAATTTGACTTGCTTCCCTGTTCTTGAAGAGTACTTGAATGTCGGGGTCTGGTGGGTGGGGGCCTGGGACACCCACTGC
1281 CCAGCATCCTCCACCCTCCTCCCTAGTCTCATAGGATCGTCACAGTGGAGGTGACATGCCTTCTCCAGTCCTGCCCCACC
1361 TGCCTCTGTGGACACATTTCCAAAGAACCCCTGGGGGTGGGACCTCCTCCATCAGTATGACTCAGCTGTTGGCCACCTGA
1441 GGACTCGGCCCCCCTGCAGGTTCCTGAAGCAACCTGACTGGGCAGTGAGCAGCATTGACCCCCACTCACCCCCAAAACAG
1521 GGCTGTGATTTCCTTAGTCCTTCCAAGCCCGACCTGGAGGATGGGTCAGACCCCTTAACTGTGAATGAGACATGATCCTG
1601 GGCTGGCTTCGCCACAAACCATGCAGAAATCTAAAAGGCCTGTTGTAGAGTGGGGGACATGCAAGCACTTTTAACTCCAT
1681 CGTACCAGGTGAACTGACCTCCGGACTCCTTTCCCACCAACTGTCAACGCCAGGATTTTGTATTCTGTTTTGTAAGGATT
1761 TAATAAAAGTCATTTAAAAATTTCTGTGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugUUGUUUUAGUGAUCAGAAGGu 5'
            |:| |   ::|| ||||||| 
Target 5' ccAGCCACTCTGCT-GTCTTCCa 3'
 318 - 339 148.00 -15.20
2 
miRNA  3' uguUGUUUUAGUGAUCA-GAAGGu 5'
             :::|  || :|||| ||||| 
Target 5' gctGTGATTTCCTTAGTCCTTCCa 3'
 1522 - 1545 128.00 -12.40
3 
miRNA  3' ugUUGUUU-UA--------GUGAUCAGAAGGu 5'
            |:|||| ||        |||  |||| || 
Target 5' tcAGCAAACATGGGGCTCCCACATGTCTCCCc 3'
 730 - 761 123.00 -13.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Min6 , HEK-293T
Disease 17347.0
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... "Induction of miR-7a significantly downregulated 39-UTR reporter activity for all five targets (Fig. 2C) ...

- Wang Y; Liu J; Liu C; Naji A; Stoffers DA, 2013, Diabetes.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' uguuguuuuagUGAUCAGAAGGu 5'
                     :|| ||||||| 
Target 5' -----------GCU-GUCUUCCa 3'
 		1 - 11
Article - Wang Y; Liu J; Liu C; Naji A; Stoffers DA
- Diabetes, 2013
Elucidating the mechanism underlying the poor proliferative capacity of adult pancreatic beta-cells is critical to regenerative therapeutic approaches for diabetes. Here, we show that the microRNA (miR)-7/7ab family member miR-7a is enriched in mouse adult pancreatic islets compared with miR-7b. Remarkably, miR-7a targets five components of the mTOR signaling pathway. Further, inhibition of miR-7a activates mTOR signaling and promotes adult beta-cell replication in mouse primary islets, which can be reversed by the treatment with a well-known mTOR inhibitor, rapamycin. These data suggest that miR-7 acts as a brake on adult beta-cell proliferation. Most importantly, this miR-7-mTOR proliferation axis is conserved in primary human beta-cells, implicating miR-7 as a therapeutic target for diabetes.
LinkOut: [PMID: 23223022]
41 mmu-miR-7a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003971 Hells helicase, lymphoid specific 2 1
MIRT053031 Eif4e eukaryotic translation initiation factor 4E 2 1
MIRT053032 Rps6kb1 ribosomal protein S6 kinase, polypeptide 1 2 1
MIRT053033 Mapkap1 mitogen-activated protein kinase associated protein 1 2 1
MIRT053034 Mknk2 MAP kinase-interacting serine/threonine kinase 2 2 1
MIRT053035 Mknk1 MAP kinase-interacting serine/threonine kinase 1 2 1
MIRT577318 Zfp26 zinc finger protein 26 1 3
MIRT578104 Nup133 nucleoporin 133 1 1
MIRT579456 Acsm2 acyl-CoA synthetase medium-chain family member 2 1 1
MIRT581045 Sike1 suppressor of IKBKE 1 1 1
MIRT582290 Nav2 neuron navigator 2 1 3
MIRT583587 Fat3 FAT atypical cadherin 3 1 1
MIRT584159 Creb1 cAMP responsive element binding protein 1 1 1
MIRT586660 Mbp myelin basic protein 1 1
MIRT587038 Milr1 mast cell immunoglobulin like receptor 1 1 1
MIRT587579 Cpm carboxypeptidase M 1 1
MIRT587868 Btn1a1 butyrophilin, subfamily 1, member A1 1 3
MIRT589879 Hdac7 histone deacetylase 7 1 1
MIRT590087 Evi2b ecotropic viral integration site 2b 1 1
MIRT590957 Stxbp2 syntaxin binding protein 2 1 1
MIRT591080 Polr2l polymerase (RNA) II (DNA directed) polypeptide L 1 1
MIRT591342 Gla galactosidase, alpha 1 1
MIRT593125 Cry2 cryptochrome 2 (photolyase-like) 1 1
MIRT594875 Magt1 magnesium transporter 1 1 1
MIRT598790 Gmeb1 glucocorticoid modulatory element binding protein 1 1 1
MIRT599234 Dcaf10 DDB1 and CUL4 associated factor 10 1 1
MIRT600587 Nipal4 NIPA-like domain containing 4 1 1
MIRT600617 Mtfmt mitochondrial methionyl-tRNA formyltransferase 1 1
MIRT600913 Faim2 Fas apoptotic inhibitory molecule 2 1 1
MIRT600985 Ddx27 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 1 1
MIRT602025 Moap1 modulator of apoptosis 1 1 1
MIRT604224 Clca2 chloride channel accessory 3A2 1 1
MIRT604422 Acmsd amino carboxymuconate semialdehyde decarboxylase 1 1
MIRT604505 Trappc13 trafficking protein particle complex 13 1 1
MIRT731319 Sp1 trans-acting transcription factor 1 1 1
MIRT731320 Parp1 poly (ADP-ribose) polymerase family, member 1 1 1
MIRT731919 Nlrp3 NLR family, pyrin domain containing 3 1 1
MIRT732184 Herpud2 HERPUD family member 2 3 1
MIRT734898 Pax6 paired box 6 3 0
MIRT734899 Neurog2 neurogenin 2 3 0
MIRT734900 Neurod1 neurogenic differentiation 1 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-7a Isoproterenol approved 3779 Quantitative real-time PCR heart 22847192 2012 up-regulated
miR-7a Chaihu Shugan San NULL NULL Microarray hippocampus 23947143 2013 up-regualted
miR-7a Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-7a-5p Gefitinib 123631 NSC715055 approved sensitive Low Non-Small Cell Lung Cancer tissue and cell line

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