miRTarBase - #MIRT054178


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pre-miRNA Information
pre-miRNA mmu-mir-125a   
Genomic Coordinates chr17: 17830812 - 17830879
Description Mus musculus miR-125a stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-125a-5p
Sequence 6| UCCCUGAGACCCUUUAACCUGUGA |29
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Ptpn7   
Synonyms BPTP-4, C920001D21Rik, HEPTP, LC-PTP, LPTP
Description protein tyrosine phosphatase, non-receptor type 7
Transcript NM_177081   
Expression
Putative miRNA Targets on Ptpn7
3'UTR of Ptpn7
(miRNA target sites are highlighted)		 
>Ptpn7|NM_177081|3'UTR
   1 CCCTCCACCAGCTCATGGGTCTCTCAGGCCTGAGACAGTGGGTCTGGGGAAGTGGGCTGTGAGCTCTGGCATGGCTTCTG
  81 CATCCGCACAGGAAAAGGATCCCTGGAATACTTTACAGTCTCAGGAAACTGCCCCAGCAAGCAACAGGAGCCAGGCCACG
 161 GGTCTTCACCTCCAACCACGACTCTGCCTTCTTCAGTGGCCCCCAGTGGAACATGCGGGAGCGTCCTGTGGCTCTGAACT
 241 TACAGATAGGAGCTGGTCCTTGCAATGGATTGAGAGAGCAGCTGGAGTGCTGGCTTATTTAGGTGCTTTCGGTGAGCTCC
 321 CTTCCTTGTCCATGACACAAAGAGTCAGTCAAACAGAATGTTCCTGAAAGCCATTCCAGATGTAGCCCTGCTGATGCGGC
 401 TGCAGAGATGGTGATTGCAAGATCCTCTTCCATTAAGGCCTTTCCCACACACAGGCCAGATGCAACAGCAGGGAAAATCC
 481 TGGTCTGACCTTGGTCAAATCTTCTCTATCCTACATAGACTGCTCCTCCCCATACACCCGTGAGTGCTGGAGGACCGGCT
 561 CAGAAGAGAATAAGGGCCTTGATTGGCCTGCCTCCATACATATCATAAAAGGAGATGCAGAGGTAACCAGCTCTAGCCGT
 641 TGGGATCACTGTATGAGCCCAGAAGTGATGATGTACCTGTCATCCTAGCACTCAGGAGTAGAAGCAGGTGGGTTGCTGCG
 721 AGTTTGAGGTCAGCTAGATCTACATTTTGAGTACCAGGCTAGCCAGGACAACATAGCAAGATTTTTTTTAAGGCAATCTT
 801 GAGCTCTCTGCTCTCTGGAGGAGATGAGGGTTGCCTCTGCTTCTATGGCTTCCTTCCTGTCATCTGCTCCCAACCACTCT
 881 CTGGGGGAAAACCCAGCACTCTCTGCTTTGCTTCTGTGACTTCAGAGCATCCTTTGGGTACCCATGAAGTATCAAAGAAA
 961 GAGGAGAGAAGAGACAAAGAGCTATGTCTGCTTCAAGAAAACCTACCTGCCGTACCTCCCCTGGCTTGTATTTCTTTGCA
1041 GAGTGCTTATCTGGTTCCAGAAGCAGGCTGGGTGGTCACCACTCCAAGCAGCATGGGGCTAAGACCTTCAGCCCTCATCA
1121 TCATCACTTCACAGAATTTCCAGGGAACTCATGATCTATCTCAGGGATGAACTATGAATCCCAGAAAGCCAGACAAACCC
1201 TGAGTCTCCCTTGACCCGGATTGGGCTGCCTGTGGGGGGGAAGGCAGAATACTATTCCTACTGTCTGGTCCTCAGCTCCT
1281 GGATGTGCTAAGTGACACAAGTCCTCCATGAAAACCCCAAGGAGTTGGAGGAGTGACTGCCTCCTTGCTTCCTGGCATCT
1361 CTCTGGAGAACTCGAAGTGCTTCTGGACATAGTGTCGAAAGCCTGTGAGCCGCACTTGGACAGGGAAGACAGGAGAATCG
1441 GGAGTTCCAGACCAGCCTTGGCGATGTGAGACCCAATCTCAAAAGCAAGCAAACAAAAAGAAACTAAGCAGCGGGGCATG
1521 GTGGTGCATACCTTTAATTCCAGCACTCGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCAAGGCCAGCCTGGTCTACAAA
1601 GTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGCCTCGAAAGAAAAAAAAAAGAAAGAAAGAAAGAAAGAAAG
1681 AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAAGAAACGAAACTAAACAAACAAACAAACAAACAAAAATAATGTGA
1761 GGCTACAACTGAGGTTAACATTTTTAATGCTCGACCTGAGCTGAGTAGGTGCTCTGCTTCTTTAGTCTTGGGGTGGTGGC
1841 TTGCACTTCCACTTTTTTTTTAAGTCCCCTGAAAGGTCTGCACTGCTGCCCAGGATAGGCTTCTAAAGTACAGGATGCTA
1921 CATGAGCTCAGAGACTGTATAGAAGGGTGCAAGGGAGCAATTGTACAGCCCTATGGGCTCCCTCCCTATACAGTCACCCC
2001 AACCCTGTTCTAGAGGGCACAGGCTCTGGAAAAGCATAATAAATAAATAAATAACCTCTTGGATGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aguGUCCAAUUUCCCAGAGUCCCu 5'
             || | |  |   |||||||| 
Target 5' actCATGATCTA---TCTCAGGGa 3'
 1147 - 1167 146.00 -15.20
2 
miRNA  3' agUGUCCAAUUUCCCAGAGUCCcu 5'
            |:|  ||| |  ||||||||  
Target 5' gaATACTTTACA--GTCTCAGGaa 3'
 106 - 127 142.00 -12.40
3 
miRNA  3' aguGUCCAAUUUCC-CAGAGUCCcu 5'
             ||| | | :||  |||||||  
Target 5' cacCAGCTCATGGGTCTCTCAGGcc 3'
 6 - 30 141.00 -15.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mouse
Disease 320139.0
Location of target site 3'UTR
Original Description (Extracted from the article) ... "miR-125a significantly down-regulated the 3芒鈧虏UTR reporters of multiple protein phosphatases ...

- Guo S; Bai H; Megyola CM; Halene S; Krause et al., 2012, Proceedings of the National Academy of Sciences of the United States of America.

Article - Guo S; Bai H; Megyola CM; Halene S; Krause et al.
- Proceedings of the National Academy of Sciences of the United States of America, 2012
Deregulation of microRNA (miRNA) expression can lead to cancer initiation and progression. However, limited information exists on the function of miRNAs in cancer maintenance. We examined these issues in the case of myeloproliferative diseases and neoplasms (MPN), a collection of hematopoietic neoplasms regarded as preleukemic, thereby representing early neoplastic states. We report here that microRNA-125a (miR-125a)-induced MPN display a complex manner of oncogene dependence. Following a gain-of-function genomics screen, we overexpressed candidate miR-125a in vivo, which led to phenotypes consistent with an atypical MPN characterized by leukocytosis, monocytosis, splenomegaly, and progressive anemia. The diseased MPN state could be recapitulated in a doxycycline-inducible mouse model. Upon doxycycline withdrawal, the primary MPN phenotypes rapidly resolved after the discontinuation of miR-125a overexpression. However, reinduction of miR-125a led to complex phenotypes, with some animals rapidly developing lethal anemia with extensive damages in the spleen. Forced expression of miR-125a resulted in elevated cellular tyrosine phosphorylation and hypersensitivity toward hematopoietic cytokines. Furthermore, we demonstrate that miR-125a targets multiple protein phosphatases. Our data demonstrate that miR-125a-induced MPN is addicted to its sustained overexpression, and highlight the complex nature of oncogenic miRNA dependence in an early neoplastic state.
LinkOut: [PMID: 23012470]
28 mmu-miR-125a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001716 Lin28a lin-28 homolog A (C. elegans) 1 1
MIRT005597 Trim71 tripartite motif-containing 71 4 3
MIRT006310 Cbx7 chromobox 7 2 1
MIRT006967 4632428N05Rik V-set immunoregulatory receptor 2 1
MIRT054177 Ptpn18 protein tyrosine phosphatase, non-receptor type 18 2 1
MIRT054178 Ptpn7 protein tyrosine phosphatase, non-receptor type 7 2 1
MIRT054179 Ppp1ca protein phosphatase 1, catalytic subunit, alpha isoform 2 1
MIRT054180 Ppp2ca protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform 2 1
MIRT054570 Traf6 TNF receptor-associated factor 6 3 1
MIRT580227 Tspan12 tetraspanin 12 2 2
MIRT582368 Mtf1 metal response element binding transcription factor 1 2 2
MIRT582450 Mgat4a mannoside acetylglucosaminyltransferase 4, isoenzyme A 2 2
MIRT584884 Antxr2 anthrax toxin receptor 2 2 2
MIRT587193 Foxk1 forkhead box K1 2 2
MIRT588848 Snrnp40 small nuclear ribonucleoprotein 40 (U5) 2 2
MIRT591275 Lars2 leucyl-tRNA synthetase, mitochondrial 2 4
MIRT592914 Madd MAP-kinase activating death domain 2 2
MIRT594893 Il1rn interleukin 1 receptor antagonist 2 4
MIRT596427 Hif1an hypoxia-inducible factor 1, alpha subunit inhibitor 3 3
MIRT599142 Dqx1 DEAQ RNA-dependent ATPase 2 2
MIRT599717 Aph1c aph1 homolog C, gamma secretase subunit 2 2
MIRT600832 Gm14137 predicted gene 14137 2 2
MIRT604902 Jmy junction-mediating and regulatory protein 2 2
MIRT605441 Stat1 signal transducer and activator of transcription 1 2 2
MIRT606092 Wdr25 WD repeat domain 25 2 2
MIRT732537 Tnfrsf1b tumor necrosis factor receptor superfamily, member 1b 4 0
MIRT734700 VDR vitamin D receptor 2 0
MIRT734824 Sox11 SRY (sex determining region Y)-box 11 2 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-125a Budesonide approved 5281004 Microarray neonatal mice liver 20145010 2010 down-regulated
miR-125a Phenethyl isothiocyanate(PEITC) NULL 16741 Microarray neonatal mice lung 20145010 2010 down-regulated
miR-125a Chaihu Shugan San NULL NULL Microarray hippocampus 23947143 2013 down-regualted
miR-125a Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated
miR-125a Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 up-regulated
miR-125a Enoxacin approved 3229 Quantitative real-time PCR HEK293 cells 18641635 2008 up-regulated
miR-125a 4-hydroxynonenal NULL 5283344 Microarray human leukemic HL-60 cell 19022373 2009 up-regulated
miR-125a Valproate approved 3121 Quantitative real-time PCR CD4+, CD25- T cells 20427269 2010 down-regulated
miR-125a Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-125a Enoxacin approved 3229 Northern blot HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-125a Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-125a 5-aza-2'-deoxycytidine (5-Aza-CdR) approved 451668 Microarray breast cancer MDA-MB231 22076154 2011 down-regulated
miR-125a-5p Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 up-regulated
miR-125a-5p Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 down-regulated
miR-125a-5p Curcumin NULL 969516 Microarray HONE1 cells 24896104 2014 down-regulated
miR-125a-5p 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-125a-5p Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-125a-5p Trastuzumab approved NULL Microarray SKBR3 cells. 22384020 2012 up-regulated
miR-125a-5p Glucocorticoid NULL NULL TaqMan low-density array Eosinophilic esophagitis 22815788 2012 up-regulated
miR-125a-5p Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-125a-5p Paclitaxel 36314 NSC125973 approved sensitive Low Colon Cancer tissue and cell line (HT-29)

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