miRTarBase - #MIRT054570


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pre-miRNA Information
pre-miRNA mmu-mir-125a   
Genomic Coordinates chr17: 17830812 - 17830879
Description Mus musculus miR-125a stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-125a-5p
Sequence 6| UCCCUGAGACCCUUUAACCUGUGA |29
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Traf6   
Synonyms 2310003F17Rik, AI851288, C630032O20Rik
Description TNF receptor-associated factor 6
Transcript NM_009424   
Expression
Putative miRNA Targets on Traf6
3'UTR of Traf6
(miRNA target sites are highlighted)		 
>Traf6|NM_009424|3'UTR
   1 CGTCCATGTACTTGTGTTCAAAAACTAGGAACCATATGGGAAAACCGTGCCTTCCATGCCTGGCCCCAGTAAACAATGTT
  81 CAAACAAGCAGTGGGAGAGGTGTAAGGCCTAGCAGCAGATGTCATCAGTGAGGTCACGAGCCACTTCTTACTGTTAACAA
 161 ATACCTGAGGCAGTTCCCATGGGAACCTACATGTCCCCTGTATCTTCAAAACGTCAACATTTGAAGGGCCTGTGGCTCAT
 241 CTGTCTGTCAGGGTACCCCTTCACTGTGCTTCCATGGGCTATTTTGTCCGTGTACTTTACTGTAAAAAAGGCCAGACTTA
 321 GCGTGCTGCAGCTCAATCGTTTAATAAGACCGGTGCCTTAAAAACTTGAGGGGTTTTTAGGACACTGATTACTATATTAA
 401 ACATGAAAATCACCACTGCCTGTGCTGGTGCCAGTAGAGAAGTTACCGCTCTGGTGTTGAGTTCTCATTTAGTTGACTCC
 481 TGTGAATTTCAGAGGCTTTGAACCATGATCCCTGGAAGGCTTAAGTTCTCAAGTACTCCCTCCTCTATAGTTCACTAAGG
 561 ATCCAGGGACTGGTTTAACCCTTACTTAGTGTGAATGTATTGTCCACTGAACACCAAGCATCCCCCACTACTTTCCTGTT
 641 TTGAAATATGCTCCAGGCGGCCTCTTCCCAGTCTGTAAGACCGCGGTCATGTGCTTGCCAACTGCTGAGTGTTACTGCCA
 721 TGGAACCTTTCTTGTCTGTCCCGTGCAGCTTGGTTTCCACAGCCGGTTGCATATCTTCTGTTGCTTGCAAACACAAAATC
 801 ACCAGCCCAAACGAGTGATTTAGCTCACTAGCCATTAAATGGCATCTCGTGGATGATGACAGCAACTCTTACAGCCAGGA
 881 AACTTCAGCCCCTCTTAACTAGCTTTTGATTTAGCTTATAAGGTTAATTGAAATAAAATTGATTTTTCTCAAGGGGTTGG
 961 AGAATTGGCTCAGTGGTTAAGAGCCTTGGCTGCTCTTCCAGAGGATCCCCAGTCTGTAACTCCAGTTCCAGGGCATCTGA
1041 CACCCTCATACAGACACTCATGCAGGCAAAACACCAGTGCACATAAAATTAAACAAACAAATAAATAAATAAATTGATTT
1121 CCTCAAAACAGAATTTATTGGAACTTGGGAAATTGTAGGTACCTGAGAGATGCCTAAACCAAGGTTGGCTATCACGGTTG
1201 TGTGGACACTCAGCTTGAGTGGTGGCTTTGTCCAGCTCAGTAGAGGTTCTGATCTGTGACCCTAATGTGGAGAGGTGACT
1281 GTCGTGCTGCTGTGTATTTGTTAATGTCCTGTACATATACAGTACTTTGGAGTCTAGTTCTCAGGGAGCCCTACGACTAG
1361 TTAGAGCCTTTGTAAGGAAGCAGAGGGGATCCTCTCCTGCTGTTTACACAAGATCAGCTATGTGTTCTGGTGGTAAGAAA
1441 GGCATCCGTGCCTTCAGCTGAATCAGAGACCCGAGCAGTGCTCTGACCTGCCCTGTTCCCAGAGAACGCTCAGAGCCTCC
1521 ACCAAGGAGTCTGTTTCTCAGCTGTAGCCAGCCAGGGCCACTTTGACCTCTTCATTTTCCCCTGCTTCCATCCTTCCCCT
1601 ATAAAGGTGAGGGGAAGACCTTGTCCCCTACCATTATCACAAGCTCATCACAGGTCTCTTCTGTTGGATCCAGGAAATGT
1681 GTGTCCCTTAGCTGTGCCTCCAGCAGCCCTGAGCTGCTTGTAGCAACTTCTGCCTAAGGAGCACTGCATGGTCTTATACT
1761 GTAGTTGTTTCCCAGTGGAGTAATAAATGTGGGCTTGTTTGTTGTTTCTTTAAAGCAAGCAGTAGCTGTGTCTATATTTA
1841 TTTAGAAATTGCCTGAAGAAGATTACTCAACTATTTGAAGACTTATTTTCTATATGCCTTTCTTAATTTTTTTATGTTAT
1921 ATGTCACCACAAAAATATGAACTCCCCAACCCCCTCTCCGTTTTTTGAAAAAGGAAATGACATTTAAGAACTTCCTCATC
2001 AGATTTCTCTTTTTAAAATATCTGTATTAGGAAGAGCAGTCGTTTCCTGCCGTGGTTTTGACTTTTTTTAAAAAAAACTC
2081 TAACATCTTTTAAAGTTTTTTTGCATAAGTTAAACTGTTCCCAGCTTTAAATTGTCCTCCCTATAGGGCAAGTTGGACTA
2161 GGTGTTTCTAGTATCCGCATTGAGAAGCCCAGTGCTGAGCCACAATACTCACTAAAAGGCTTTCCCCGTAGAGGTGTGAC
2241 TGCCCCTAACTGCTAACACGGATGGTTCACTGCAGTGTAATGTCCATCCGCTAGAATACACCTCAGGTAGTTTTAGAACT
2321 TGCAGCATTTGGTGTTTGTAATAAGCCAACCAGTTACTTTATGTTACTCAATTGCCACGAATGCAGAGTAAAACTAATCA
2401 AGCTGACATTCAAGGTCAACACTTAGTAAGGTCAACTCAGGATCAAGTCTTAGCCTAGAAAGCCGCTTTCTTTACTTCAC
2481 CACTTTCTGAACATTCTCTTTGTACCAATGGGCCTATAAGAATCCGTATAGTCCAGAGTGCATTGGCCATCTTTCCTTAC
2561 CAATCTAGAACACTGCTGAATTTAAAGTTGTTTCTTCTTAGAAAAATGCCTACCTTACTATTGAAGATTTTTCCCCAAGT
2641 CATATATTTCCCTCTTAGAAATCAGGCCAGACGGCAGTTCTAGTTTGGAAGTTGGTTACAGTCCTTTGGCTGTTACCATC
2721 TCTAGCCATTCTGCTTTCTTCTGGAGAATGAAGAGGAGAAAAGTGCATTAAAGTACAAAAGGTGTCCTCTCACCCTCGGA
2801 AGATCAACTGACAGGTGTTGGATGATCTCCAACAAGTAAATTTTGTGACCAGTATAAAGTTGAATTTGTACCAATATCAA
2881 ACAAAGTCTGACCAATGTAAATTATGTGCACAATTAGAATATCTTCTCCTTAAGGAGAGGTTGCTTGTTTCTGCTTTACC
2961 TGGAGTTTCCTTCTTTCGCATGTGACTGGAAAACGTTTTAACTTTAACTATCGAGGTGATTCTTACTTAAGACTTTGAAG
3041 TGCTTTTCTCTCTTTTTCTGTCGTTAACACACATCTTTTCTTGACTTGACTCAAATTCTCGCCATTGTTACAGTTTTTTA
3121 TGGGGTGTTTGGTGATTAGTTTGCTGGCTGCCTTGAGGGAGTGAACAGGGCACGGTCAAGCGTCGTTTGATTGTCTGTTG
3201 AAATACTCTTTAAATGTCGGCATTCTCAGGGTAACTGTCATTTGTTTCAAAGTTGATGTGATTGTCTGGGAAATGGATGG
3281 ATGCTTCCCAATTCCCAGAATCCAGAAAAATGAAACCAGATGTGATCAACCTGAACTTGGGACACTCTCGGTCACAAGCG
3361 TTGAAGTCACTCAAAAAGGACTAAGCTAGTTATTTCTCTGTGGGTCCTCTGTGTCTTTGATGTTTTAAATTGCTCAGCCC
3441 CGCCCCAATAAATAAATAAATAAATAAGAAAAGAAAAGAGTTGTAGTTTTTCACATTGTGGAATGTGGAGAGGAACTCCT
3521 TTTCCTGTCCTGTGTCTCCTCAGCGGAGCCCAGCCCTGCCTGACACAGGAGAAAAGGGTGGCCTGTTGGTCACCTGCCCT
3601 TCAGAATGTAGCCCCATCTGACTCCTAAAACCCCAGTTTCCTTCAGTGCAGGCTCCAGGAGAGGGCAGAGACCCCATTCT
3681 GGTCACTGCTGAACCCCTGTTTTTAGCATACTGTGCATGGGCCTGGCCAATAGTCACAAGCTTTAATGGGAGCCAGGGCA
3761 GAAGCTGACTGGCTGCTGGGTAGCCTACTTGTCATGTAAGTCAGTTGGTAAAGTGAGAGTGTTCTTTTTTCTGCTTTTCT
3841 CCCGGGACTTTGCTACTGCAGTTCTCAAACATGGAAGTGAGTTTAAGACCTAGTGAACACCTCCCACCTAGGATCTGCAG
3921 TGACATTGGGTGTGCTCTGATTTAATGCTTCTATCATGTAAATTCTAATTTCTCCTTAAGGCTGTTCAATCCTGAAATAA
4001 TTAAACAACTTGAAGTTGTATAAAATTCTCCTTGGAAACTTGTGATATTTTATTGTAATTTATCTTGTAGCTTCTGCTTT
4081 ATGCCAACTTAAAATTTGTGGAAATGTTGTGAGGAACTTTACTCTTATGTCTTTGTCTACAGGAGTATTTTTATAAAGGA
4161 TTTATTTGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agUGUC---CAAUUUC----CCAGAGUCCCu 5'
            ||||    ||::||    | |||||||| 
Target 5' atACAGTACTTTGGAGTCTAGTTCTCAGGGa 3'
 1317 - 1347 160.00 -20.40
2 
miRNA  3' agUGUCCAAUUU-C--CC---AGAGUCCCu 5'
            ||   ||||| |  ||   |||||||| 
Target 5' atACTCTTTAAATGTCGGCATTCTCAGGGt 3'
 3203 - 3232 156.00 -15.00
3 
miRNA  3' agUGUCCAAUU-UCCCA---GAGUCCcu 5'
            |||  |||: | |||   ||||||  
Target 5' caACA-CTTAGTAAGGTCAACTCAGGat 3'
 2417 - 2443 137.00 -14.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions H9c2 (2-1)
Disease myocardial ischaemia reperfusion injury
Tools used in this research unspecified
Original Description (Extracted from the article) ... miR-125 protects the myocardium form I/R injury by preventing p53-mediated apoptotic signalling and suppressing TRAF6-mediated NF-kB activation ...

- Wang X; Ha T; Zou J; Ren D; Liu L; Zhang X; et al., 2014, Cardiovascular research.

Article - Wang X; Ha T; Zou J; Ren D; Liu L; Zhang X; et al.
- Cardiovascular research, 2014
AIMS: The present study examined the role of microRNA-125b (miR-125b) in myocardial ischaemia/reperfusion (I/R) injury. We constructed lentivirus-expressing miR-125b (LmiR-125b) and developed transgenic mice with overexpression of miR-125b. METHODS AND RESULTS: LmiR-125b was transfected into mouse hearts through the right common carotid artery. Lentivirus vector (LmiR-Con) served as vector control. Untreated mice served as I/R control. Sham operation served as sham control. Seven days after transfection, the hearts were subjected to ischaemia (45 min) followed by reperfusion (4 h). Myocardial infarct size was analysed by 2,3,5-triphenyltetrazolium chloride staining. In separate experiments, hearts were subjected to ischaemia (45 min) followed by reperfusion for up to 7 days. Cardiac function was measured by echocardiography before, as well as 3 and 7 days after myocardial I/R. Increased expression of miR-125b significantly decreased I/R-induced myocardial infarct size by 60% and prevented I/R-induced decreases in ejection fraction (EF%) and fractional shortening (%FS). Transgenic mice with overexpression of miR-125b also showed the protection against myocardial I/R injury. Increased expression of miR-125b attenuated I/R-induced myocardial apoptosis and caspase-3/7 and -8 activities. Western blot showed that increased expression of miR-125b suppresses p53 and Bak1 expression in the myocardium. In addition, transfection of LmiR-125b decreased the levels of TNF receptor-associated factor 6 (TRAF6) and prevented I/R-induced NF-kappaB activation. CONCLUSION: miR-125 protects the myocardium from I/R injury by preventing p53-mediated apoptotic signalling and suppressing TRAF6-mediated NF-kappaB activation.
LinkOut: [PMID: 24576954]
28 mmu-miR-125a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001716 Lin28a lin-28 homolog A (C. elegans) 1 1
MIRT005597 Trim71 tripartite motif-containing 71 4 3
MIRT006310 Cbx7 chromobox 7 2 1
MIRT006967 4632428N05Rik V-set immunoregulatory receptor 2 1
MIRT054177 Ptpn18 protein tyrosine phosphatase, non-receptor type 18 2 1
MIRT054178 Ptpn7 protein tyrosine phosphatase, non-receptor type 7 2 1
MIRT054179 Ppp1ca protein phosphatase 1, catalytic subunit, alpha isoform 2 1
MIRT054180 Ppp2ca protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform 2 1
MIRT054570 Traf6 TNF receptor-associated factor 6 3 1
MIRT580227 Tspan12 tetraspanin 12 2 2
MIRT582368 Mtf1 metal response element binding transcription factor 1 2 2
MIRT582450 Mgat4a mannoside acetylglucosaminyltransferase 4, isoenzyme A 2 2
MIRT584884 Antxr2 anthrax toxin receptor 2 2 2
MIRT587193 Foxk1 forkhead box K1 2 2
MIRT588848 Snrnp40 small nuclear ribonucleoprotein 40 (U5) 2 2
MIRT591275 Lars2 leucyl-tRNA synthetase, mitochondrial 2 4
MIRT592914 Madd MAP-kinase activating death domain 2 2
MIRT594893 Il1rn interleukin 1 receptor antagonist 2 4
MIRT596427 Hif1an hypoxia-inducible factor 1, alpha subunit inhibitor 3 3
MIRT599142 Dqx1 DEAQ RNA-dependent ATPase 2 2
MIRT599717 Aph1c aph1 homolog C, gamma secretase subunit 2 2
MIRT600832 Gm14137 predicted gene 14137 2 2
MIRT604902 Jmy junction-mediating and regulatory protein 2 2
MIRT605441 Stat1 signal transducer and activator of transcription 1 2 2
MIRT606092 Wdr25 WD repeat domain 25 2 2
MIRT732537 Tnfrsf1b tumor necrosis factor receptor superfamily, member 1b 4 0
MIRT734700 VDR vitamin D receptor 2 0
MIRT734824 Sox11 SRY (sex determining region Y)-box 11 2 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-125a Budesonide approved 5281004 Microarray neonatal mice liver 20145010 2010 down-regulated
miR-125a Phenethyl isothiocyanate(PEITC) NULL 16741 Microarray neonatal mice lung 20145010 2010 down-regulated
miR-125a Chaihu Shugan San NULL NULL Microarray hippocampus 23947143 2013 down-regualted
miR-125a Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated
miR-125a Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 up-regulated
miR-125a Enoxacin approved 3229 Quantitative real-time PCR HEK293 cells 18641635 2008 up-regulated
miR-125a 4-hydroxynonenal NULL 5283344 Microarray human leukemic HL-60 cell 19022373 2009 up-regulated
miR-125a Valproate approved 3121 Quantitative real-time PCR CD4+, CD25- T cells 20427269 2010 down-regulated
miR-125a Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-125a Enoxacin approved 3229 Northern blot HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-125a Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-125a 5-aza-2'-deoxycytidine (5-Aza-CdR) approved 451668 Microarray breast cancer MDA-MB231 22076154 2011 down-regulated
miR-125a-5p Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 up-regulated
miR-125a-5p Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 down-regulated
miR-125a-5p Curcumin NULL 969516 Microarray HONE1 cells 24896104 2014 down-regulated
miR-125a-5p 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-125a-5p Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-125a-5p Trastuzumab approved NULL Microarray SKBR3 cells. 22384020 2012 up-regulated
miR-125a-5p Glucocorticoid NULL NULL TaqMan low-density array Eosinophilic esophagitis 22815788 2012 up-regulated
miR-125a-5p Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-125a-5p Paclitaxel 36314 NSC125973 approved sensitive Low Colon Cancer tissue and cell line (HT-29)

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