miRTarBase - #MIRT054600


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pre-miRNA Information
pre-miRNA mmu-mir-325   
Genomic Coordinates chrX: 105379082 - 105379179
Synonyms Mirn325, mmu-mir-325, Mir325
Description Mus musculus miR-325 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-325-3p
Sequence 54| UUUAUUGAGCACCUCCUAUCAA |75
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Arc   
Synonyms Arc3.1, C86064, arg3.1, mArc
Description activity regulated cytoskeletal-associated protein
Transcript NM_018790   
Expression
Putative miRNA Targets on Arc
3'UTR of Arc
(miRNA target sites are highlighted)		 
>Arc|NM_018790|3'UTR
   1 AGGGGCCAGCCCAGGGTCCCCAGCCCGCCTGCCACACCCAGCCTGTGGCTTTTGCCAACTAGGACTTGAGCTGGGGCTGA
  81 CTCCCAAAGGGATGCCCTGTCCAGCCAGACATCTACTCACCCACTGACCTGGCCTGACTCACAACTGCCACACAACCATG
 161 CTACATGGACTGAACATCAAGAAGCCCCTTTCCCATAGGGCTCCCACCTGCCGCCTACCCCTCATCTGTCTGCCCTGGTC
 241 CTGGCCCCCAGCCCCATTGGCCTCACCCTCTACACTCTCAGACCATCACAGAACACGATCTGGCTTCCTCATTCTGCTCC
 321 AGTGTCCAGGGCTCTTTGGGTAATCAAGAAACCAAGTGTCTGAAAGGCAACAAAAAGTAGGCACCAAAACCCAGGGGACA
 401 TCCTAGGGCAGATGCTAAAGCAGAGATGCTGAGGGAACCTCAACTTCCGGGGATGCAGCCCACTCCTGCAGACACAGCAG
 481 ATCCAGCTGGTACCCTAGCTGCCGCCCAGGGCAACCGGCCAGTCTTGGGCAGCATAGCTCCCCTCCCAGGGGTGAGCTGA
 561 AGCCACAAATGCAGCTGAAGCAGCAGACCTGACATCCTGGCACCTCCTGGCCCCCAGCAGTGATTCATACCAGTGAAGAA
 641 GAGCAGAGCTCAGCTCCGTGACTCAGCCATGGCAGGCGGAGGGTCCCCGAGGGGCTGAGTCCTCACACCCAGCCGAGGCA
 721 GCGGCTGGAGCCTACAGAGCCAGGAGAATGACACCAGGTCTCAAGGCTGCTGAGAAGTCTTTGCTGCCATGTCTGGAAAG
 801 GGCATCACCACACCACCAGCACCAGCACCATCCTCTCTTCTCCTGAAGCTGCCTACATGGGTTCCAAGACACTTTCAAGG
 881 CAGAGAAAACAAGATTACAGAGAGGAGGTGCCTGGCAGGGGGCAGCACCCCAGCTCAGCCCAAGAGCTGAAGGTGAAGAC
 961 AAGCCAGCATGAAACCACGGGTCTGCCATGATGCCCGCCCCGCTGGCCGCTCACTAGCTGCCTGCCATTAGCCTCTGCAG
1041 CTTGAGCAGGGTCTGTACCCTTGGTCCTCTTGGCACAGAGCCCAGCTCGCTGCATGGCCTTTGGCTCCCCGACCAGACCC
1121 TTGCAGGAGCCTTAAGGCTTGGGCCCCTGCCCAGCCTGATCTTTCCTGCTGTGCCCTGCCTGCCAGGTCAAGCCCAGTCC
1201 CAGGAGACCCCAGGCCTTGGCTCCTAGGCTGTTCCATGAACCTCCCTGACCTGCCTGGTGATTGCCCAGCTGAAACCTCA
1281 GCCAAGCCCCAGCTCCAATTACCTTGTGCTGGTAGCTGCTTGTGTCTGCAGTCTGAGTAGGCCTTGTTGCGGTTCCTCCA
1361 TCTGCCTGGTCTATTGGTGTTCTGAGACCAATTCCACTGATGTTCTGACAGATCCTCCACCCTGTGCCCCTGCCAGCCCC
1441 CACAAGTTTATTTTTGCACAAAAGCCATGACCCATACTCATTTGGCTGGCATAGGGTGTGTAGGTAGGCCCTGGGGACTA
1521 GGGAGACCCTGGAGATCTCAAGAGAGTGTGGCTATCCCCTATTTTCACCAAGCCTTGAATATCCAGCCAGGCTGTCTGCC
1601 CATACCATCTTACCTCAAAGACAGATATATATCTATATATGATTTTGTTAATAAAACTATGAAACTTATT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aacuauccUCCACGAGUUAUUu 5'
                  :|||  |||| || 
Target 5' ggctctttGGGTAATCAAGAAa 3'
 330 - 351 118.00 -5.70
2 
miRNA  3' aacuauCCUCCACGAGUUAUUu 5'
                |||| | ||||| |: 
Target 5' gaccctGGAGAT-CTCAAGAGa 3'
 1525 - 1545 111.00 -8.80
3 
miRNA  3' aacuAUCCUCCACGAGUUAUUu 5'
              |||  | ||:|| ||:| 
Target 5' ctccTAG--GCTGTTCCATGAa 3'
 1221 - 1240 110.00 -9.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293
Location of target site 3'UTR
Tools used in this research RNAhybrid
Original Description (Extracted from the article) ... ARC is a target of miR-325 ...

- Bo L; Su-Ling D; Fang L; Lu-Yu Z; Tao A; et al., 2014, Cell death and differentiation.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' aacuaUCCUCCACGAGU-UAUUu 5'
               :|||||  || | | :| 
Target 5' uugcaGGGAGGACCUGAGAGGA- 3'
 		8 - 29
Article - Bo L; Su-Ling D; Fang L; Lu-Yu Z; Tao A; et al.
- Cell death and differentiation, 2014
Autophagy is required for the maintenance of cardiomyocytes homeostasis. However, the abnormal autophagy could lead to the development of heart failure. Autophagy is enhanced during myocardial ischemia/reperfusion; it remains to elucidate the molecular regulation of autophagy. We report here that miR-325, ARC and E2F1 constitute an axis that regulates autophagy. Our results showed that miR-325 expression is upregulated upon anoxia/reoxygenation and ischemia/reperfusion. Cardiomyocyte-specific overexpression of the miR-325 potentiates autophagic responses and myocardial infarct sizes, whereas knockdown of miR-325 inhibited autophagy and cell death. We searched for the downstream mediator of miR-325 and identified that ARC is a target of miR-325. ARC transgenic mice could attenuate autophagy and myocardial infarction sizes upon pressure-overload-induced heart failure, whereas ARC null mice exhibited an increased autophagic accumulation in the heart. The suppression of ARC by miR-325 led to its inability to repress autophagic program. In exploring the molecular mechanism by which miR-325 expression is regulated, our results revealed that the transcription factor E2F1 contributed to promote miR-325 expression. E2F1 null mice demonstrated reduced autophagy and myocardial infarction sizes upon ischemia/reperfusion. Our present study reveals a novel autophagic regulating model that is composed of E2F1, miR-325 and ARC. Modulation of their levels may provide a new approach for tackling cardiac failure.
LinkOut: [PMID: 24531537]
27 mmu-miR-325-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT054600 Arc activity regulated cytoskeletal-associated protein 3 1
MIRT577829 Rinl Ras and Rab interactor-like 1 1
MIRT577905 Prr11 proline rich 11 1 1
MIRT578380 Krtap6-1 keratin associated protein 6-1 1 1
MIRT578550 Hsd17b1 hydroxysteroid (17-beta) dehydrogenase 1 1 1
MIRT578770 Gemin8 gem nuclear organelle associated protein 8 1 1
MIRT580288 Trhr thyrotropin releasing hormone receptor 1 1
MIRT581518 Ptprd protein tyrosine phosphatase, receptor type, D 1 1
MIRT582779 Kif1c kinesin family member 1C 1 1
MIRT585752 Stard6 StAR-related lipid transfer (START) domain containing 6 1 1
MIRT587310 Ephx3 epoxide hydrolase 3 1 4
MIRT588735 Taok3 TAO kinase 3 1 1
MIRT592550 Mup7 major urinary protein 7 1 1
MIRT592592 Mup13 major urinary protein 13 1 1
MIRT594207 Wdr12 WD repeat domain 12 1 1
MIRT594462 Epyc epiphycan 1 1
MIRT594693 Atp11b ATPase, class VI, type 11B 1 1
MIRT594929 Gjb2 gap junction protein, beta 2 1 1
MIRT594934 Frem3 Fras1 related extracellular matrix protein 3 1 1
MIRT595784 Hlf hepatic leukemia factor 1 1
MIRT595846 Set SET nuclear oncogene 1 1
MIRT595897 Cd69 CD69 antigen 1 1
MIRT603010 Klk8 kallikrein related-peptidase 8 1 1
MIRT603579 Ppm1k protein phosphatase 1K (PP2C domain containing) 1 1
MIRT604052 Esf1 ESF1 nucleolar pre-rRNA processing protein homolog 1 1
MIRT736870 GHRHR growth hormone releasing hormone receptor 2 0
MIRT756335 Gsdmd gasdermin D 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-325 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells CLY cells 20955366 2011 down-regulated
miR-325 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells HT-29 cells 20955366 2011 down-regulated
miR-325 Cisplatin approved 84093 Microarray CNE cells 22614822 2012 up-regulated
miR-325 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) NULL 16078949 Microarray hepatocytes 23107762 2013 up-regulated
miR-325-3p Urocortin 2 NULL 56843276 Quantitative real-time PCR anterior pituitary cells 22252941 2012 up-regulated

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