pre-miRNA Information | |
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pre-miRNA | hsa-mir-5590 |
Genomic Coordinates | chr2: 134857820 - 134857873 |
Description | Homo sapiens miR-5590 stem-loop |
Comment | None |
RNA Secondary Structure |
Mature miRNA Information | ||||||||||||||||||||||||||||
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Mature miRNA | hsa-miR-5590-5p | |||||||||||||||||||||||||||
Sequence | 1| UUGCCAUACAUAGACUUUAUU |21 | |||||||||||||||||||||||||||
Evidence | Not_experimental | |||||||||||||||||||||||||||
Experiments | ||||||||||||||||||||||||||||
SNPs in miRNA |
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Putative Targets |
Gene Information | |||||||||||||||||||||
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Gene Symbol | PIP4K2C | ||||||||||||||||||||
Synonyms | PIP5K2C | ||||||||||||||||||||
Description | phosphatidylinositol-5-phosphate 4-kinase type 2 gamma | ||||||||||||||||||||
Transcript | NM_001146259 | ||||||||||||||||||||
Other Transcripts | NM_001146258 , NM_001146260 , NM_024779 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on PIP4K2C | |||||||||||||||||||||
3'UTR of PIP4K2C (miRNA target sites are highlighted) |
>PIP4K2C|NM_001146259|3'UTR 1 GAGACTGCCTGGTTCTCTCTGATGTTCAAGGTGGTGGGGTTCTGAGACACTTGGGGGAATTGTGGGGATATTCTAGCCAC 81 CAGTTCTCTTCTTCCTTTGCTAAATTCAGGCTGCAGGCTCCTTCCATCCAGATAACTCCATCCTGTCGAGTAGGCTCTTT 161 CTGACCCTCAGAAATACATTGTCCTTTTTCCTCTTTGCCCATTTTTCTTCCCTCTCTTCCTCCCCATGAGAAGTCTGCTT 241 GTAGTATTAGAATGTTATTGTTGACTCTCTCCCAAGTGCCTTGATCTTTGTAATATCTCCTGTTGTTTCTATGATATAGG 321 AGCTAGGGGAAGGGGGTTGTTTGCCTTCTTCAGGACCTGACTGGACAGATGGACCTGGCTCAAGCAACTACTCTGGATGC 401 ACTTTGCTGTGTGGGATGAACTAAAAGTGTCTGAATTTTGCTGATAACTTTATAAAACTCACTATGGCATGCTTCCCTCC 481 TGGTGGGCCCTAGGATGGATGACACTCAAGATACTACAGATGTGGGTGCAGGCATGCACACACACGATGGAATATGGCCA 561 TTCCTACACAGGTGGGGTAGAGAGTGGGTCAGCAGCCTGGCACCTCACAGAGGTGGGACCTAAGAGGACTCATGATTATG 641 CAGAGAATTGGATTGGGTCTCTGTCATAGATTGAGTAATCTCTTCCCTTACCTCAATTCCATCTCCACCCATCTCTACAT 721 CTGGGCACAGCAACCCAGAGATGGCCAAAAGCATTCAAGCCTGGGGGAAGATGTTTGACTATTGCTGCTCTTCACCAGAA 801 CCTCACACCTCTCCTGGGACTGGAACCCTTCAGTGGGTGTGTGGCCAGTTTTGGAGGCTGGAATGATGGGCCAGGGTGTA 881 GGATTCATTCTCCATGTAAAGTTTCCTTTCATCCTGCCTAGCCATCCCCAAGGTTTATTTCCAGAAGAAAGGAATATCTC 961 TACTTGGATCAATTCTGGTCATTTCAAGAGGATGGAGGCCTCAAGTGTGGGAACTTCCCCTACTCCCTGGATGTGTGTAC 1041 CTAGCACACTTCCTTCTCCCACCCCTTTTTCCAGTTGGATTTGTTTTTCTGTTCTCTTCTGTCCTGTCTTATACTGCAAC 1121 TGTGTCTCCTAGGGGACAGATGGCCTTCTTTGTCATCTTCACTCTCCACCCCCAGAGAGGAGTCAGAGCCATAACTCAAT 1201 CACTCAGCCCCTCCAAAGATAGTTGATGTGTGATAATCTCATAATGTTGAGAACCCTGATGAGATACATTGTCTTCCTCT 1281 CCCTACAATGCCTCTGGGGCCAAGGCACCCATTCTTCTTGCTATCCTCCATCCCCCTTGAGGCTTCCACTTTTTTTTTTT 1361 TTAGACATAAAGCTGGGCATCAGCAACTGGCCTGTGGTGATGCAAAGCTGCTTTGCTCTGTATCTGGCTGGACTGATCTG 1441 TCTCACAAGAAGCCATGAGGCCATAGGGAGAAGCTCCCTCTCCCCTTCATCTTCTGCTCCAAAGGTGGTAGCAAGAGGAG 1521 TACCCAGTTAGGGGTTGGAGCCCCCATATAACATCTTCCTGTCAGAAGACTGATGGATCTTTTTCATTCCAACCATCTCC 1601 CTTTCCCCCGATGAATGCAATAAAACTCTGTGACACCAGCAACCATTGCTCTTTAGAAATGGGTTTTCTGATCATATGGC 1681 TGATGTGTTATGGGCAGTATGGATGTCTTCATTTGTTGCTTCTGTTTTTCATCTTTTTTGTTTTATTAATAAAAATTTAT 1761 GTATTTGCTCCTGTTACTATAATAATACAGGGAATAAATTATTCAATCCAAATTTCTGTACAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | HEK293 | ||||||
Disease | 79837.0 | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
"PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1
... - Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Kishore S; Jaskiewicz L; Burger L; Hausser et al. - Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
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CLIP-seq Support 1 for dataset GSM714644 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | HEK293 / completeT1, repA |
Location of target site | ENST00000354947.5 | 3UTR | AUAACUUUAUAAAACUCACUAUGGCAUG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21572407 / GSE28865 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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69 hsa-miR-5590-5p Target Genes:
Functional analysis:
ID | Target | Description | Validation methods | |||||||||
Strong evidence | Less strong evidence | |||||||||||
MIRT056313 | WAC | WW domain containing adaptor with coiled-coil | 2 | 6 | ||||||||
MIRT066181 | PIP4K2C | phosphatidylinositol-5-phosphate 4-kinase type 2 gamma | 2 | 2 | ||||||||
MIRT095612 | NR3C1 | nuclear receptor subfamily 3 group C member 1 | 2 | 2 | ||||||||
MIRT099166 | MYLIP | myosin regulatory light chain interacting protein | 2 | 2 | ||||||||
MIRT107578 | VLDLR | very low density lipoprotein receptor | 2 | 2 | ||||||||
MIRT179602 | CAPZA1 | capping actin protein of muscle Z-line alpha subunit 1 | 2 | 8 | ||||||||
MIRT241303 | ZC3H12C | zinc finger CCCH-type containing 12C | 2 | 8 | ||||||||
MIRT244620 | MCM4 | minichromosome maintenance complex component 4 | 2 | 2 | ||||||||
MIRT270705 | SBNO1 | strawberry notch homolog 1 | 2 | 4 | ||||||||
MIRT453616 | SNRPE | small nuclear ribonucleoprotein polypeptide E | 2 | 4 | ||||||||
MIRT453653 | RAB6C | RAB6C, member RAS oncogene family | 2 | 2 | ||||||||
MIRT464159 | VMP1 | vacuole membrane protein 1 | 2 | 15 | ||||||||
MIRT477720 | EEF1A1 | eukaryotic translation elongation factor 1 alpha 1 | 2 | 2 | ||||||||
MIRT484301 | ENDOD1 | endonuclease domain containing 1 | 2 | 4 | ||||||||
MIRT496069 | GLCCI1 | glucocorticoid induced 1 | 2 | 2 | ||||||||
MIRT496338 | TMEM81 | transmembrane protein 81 | 2 | 2 | ||||||||
MIRT496653 | PITPNM3 | PITPNM family member 3 | 2 | 2 | ||||||||
MIRT499098 | DENND4C | DENN domain containing 4C | 2 | 8 | ||||||||
MIRT499862 | SVOP | SV2 related protein | 2 | 12 | ||||||||
MIRT504913 | CD38 | CD38 molecule | 2 | 4 | ||||||||
MIRT515401 | WDR72 | WD repeat domain 72 | 2 | 4 | ||||||||
MIRT518156 | TMEM133 | transmembrane protein 133 | 2 | 2 | ||||||||
MIRT518558 | GDPD1 | glycerophosphodiester phosphodiesterase domain containing 1 | 2 | 2 | ||||||||
MIRT518636 | NOM1 | nucleolar protein with MIF4G domain 1 | 2 | 2 | ||||||||
MIRT518724 | ABCG8 | ATP binding cassette subfamily G member 8 | 2 | 2 | ||||||||
MIRT524301 | CTC1 | CST telomere replication complex component 1 | 2 | 4 | ||||||||
MIRT524472 | CHRM3 | cholinergic receptor muscarinic 3 | 2 | 4 | ||||||||
MIRT527562 | ADCY7 | adenylate cyclase 7 | 2 | 2 | ||||||||
MIRT532694 | TCN2 | transcobalamin 2 | 2 | 4 | ||||||||
MIRT534640 | RNF6 | ring finger protein 6 | 2 | 2 | ||||||||
MIRT537163 | GGCX | gamma-glutamyl carboxylase | 2 | 2 | ||||||||
MIRT539360 | AFF4 | AF4/FMR2 family member 4 | 2 | 2 | ||||||||
MIRT547023 | PPP1CB | protein phosphatase 1 catalytic subunit beta | 2 | 2 | ||||||||
MIRT548155 | FRAT2 | FRAT2, WNT signaling pathway regulator | 2 | 2 | ||||||||
MIRT550127 | ZNF138 | zinc finger protein 138 | 2 | 2 | ||||||||
MIRT558473 | DBN1 | drebrin 1 | 2 | 2 | ||||||||
MIRT566862 | LRRC58 | leucine rich repeat containing 58 | 2 | 2 | ||||||||
MIRT567246 | HSP90AA1 | heat shock protein 90 alpha family class A member 1 | 2 | 2 | ||||||||
MIRT572848 | BRPF3 | bromodomain and PHD finger containing 3 | 2 | 2 | ||||||||
MIRT574914 | Vmp1 | vacuole membrane protein 1 | 2 | 9 | ||||||||
MIRT606849 | RAB7A | RAB7A, member RAS oncogene family | 2 | 2 | ||||||||
MIRT611274 | RBMXL1 | RNA binding motif protein, X-linked like 1 | 2 | 2 | ||||||||
MIRT612328 | TRPS1 | transcriptional repressor GATA binding 1 | 2 | 2 | ||||||||
MIRT612415 | SP1 | Sp1 transcription factor | 2 | 2 | ||||||||
MIRT614791 | RRAGC | Ras related GTP binding C | 2 | 2 | ||||||||
MIRT618449 | SERPINA3 | serpin family A member 3 | 2 | 2 | ||||||||
MIRT621154 | MICALCL | MICAL C-terminal like | 2 | 2 | ||||||||
MIRT622141 | SOX4 | SRY-box 4 | 2 | 2 | ||||||||
MIRT623504 | KCNK5 | potassium two pore domain channel subfamily K member 5 | 2 | 2 | ||||||||
MIRT623598 | IPO9 | importin 9 | 2 | 2 | ||||||||
MIRT624075 | EBF1 | early B-cell factor 1 | 2 | 2 | ||||||||
MIRT639792 | MVK | mevalonate kinase | 2 | 2 | ||||||||
MIRT641109 | ZNF274 | zinc finger protein 274 | 2 | 2 | ||||||||
MIRT641579 | RFX1 | regulatory factor X1 | 2 | 2 | ||||||||
MIRT643095 | NDUFB5 | NADH:ubiquinone oxidoreductase subunit B5 | 2 | 2 | ||||||||
MIRT645141 | CUBN | cubilin | 2 | 2 | ||||||||
MIRT646461 | PRDM10 | PR/SET domain 10 | 2 | 2 | ||||||||
MIRT650815 | HMOX1 | heme oxygenase 1 | 2 | 2 | ||||||||
MIRT653073 | ST8SIA4 | ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 | 2 | 2 | ||||||||
MIRT657675 | GPR26 | G protein-coupled receptor 26 | 2 | 2 | ||||||||
MIRT665824 | TIMM8B | translocase of inner mitochondrial membrane 8 homolog B | 2 | 2 | ||||||||
MIRT695592 | TMEM199 | transmembrane protein 199 | 2 | 2 | ||||||||
MIRT698039 | TRPM7 | transient receptor potential cation channel subfamily M member 7 | 2 | 2 | ||||||||
MIRT701015 | PCGF5 | polycomb group ring finger 5 | 2 | 2 | ||||||||
MIRT701357 | NR4A3 | nuclear receptor subfamily 4 group A member 3 | 2 | 2 | ||||||||
MIRT707114 | NWD1 | NACHT and WD repeat domain containing 1 | 2 | 2 | ||||||||
MIRT718661 | HNF4A | hepatocyte nuclear factor 4 alpha | 2 | 2 | ||||||||
MIRT719901 | SERP1 | stress associated endoplasmic reticulum protein 1 | 2 | 2 | ||||||||
MIRT720564 | C1RL | complement C1r subcomponent like | 2 | 2 |