miRTarBase - #MIRT067331


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pre-miRNA Information
pre-miRNA hsa-mir-5697   
Genomic Coordinates chr1: 9967381 - 9967458
Description Homo sapiens miR-5697 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-5697
Sequence 10| UCAAGUAGUUUCAUGAUAAAGG |31
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1331648425 1 dbSNP
rs1473721376 8 dbSNP
rs1182430201 14 dbSNP
rs1412783585 21 dbSNP
Putative Targets

Gene Information
Gene Symbol SESN2   
Synonyms HI95, SES2, SEST2
Description sestrin 2
Transcript NM_031459   
Expression
Putative miRNA Targets on SESN2
3'UTR of SESN2
(miRNA target sites are highlighted)		 
>SESN2|NM_031459|3'UTR
   1 CTCCTGAGCAGGACCTGGGCCCGGTTCAGCTCCCCACAAGGACTTCTCTGTCTGGAGACAGCCCCAGACCCTTTTGTGTC
  81 CCATGCCCACCCTCCCCACGCTGCAGTGGGCTTGTGTGTGATGTGCAGTCCCGAAGCCACACCCTCCCTTTTCCTCACTG
 161 GAATGGACAGTTCATTGCACTGACTCTGGGATCTCAGCCCTGCTCCTGGGAGCTGGAAGAGCACTTGGAGATCCTAAGGG
 241 ACCACACCCTTCCTCCTTCCCCTGCCCACAGAGGCAGAGGGCACAGGAAAGAAGCCGGGCCAAGCTCGGAATTAATGTGC
 321 CACAAGTGTTGTGGCCTTCCTGAACTGGGAAGTCCCTGGCTGGCCCCCGGGGGAGAGGGGCAAATGCCTCCGGGACTGAC
 401 ACTCCAGGCAGCTTTGCCTTCTCTCCCCTGTCATTTCCAGATTTCATTACCTCCTACTTGCCATTCACCCATCAATGTGA
 481 AAGTCAGGGTCACAGCTGGTCTGTGTGTCCAGTTCCCTAAAAGCCTGTTCTGTTGGGCAGCCTGAGGCTGTTGCCCGAAT
 561 CCTAGTTCAGTTTTTTGACTTCCTTTGCCCTTTTTCCCTTTTCTCCATGCTTAATGGTGTGAGGCGTCAGGAGAGAGGCC
 641 AAGTACATAAAAAAAAAAAAAGCAGATTATCTCTAGAGAGTTTGAGCCTTTGCTGGTCACATTGCCTTCTGAAGAGGAGG
 721 GAGTATTAGATTATAAATCCTCTTTATTTTGGTCCTTTATGCTTGAGGTTCCAACCTGGAGCCACAGTGTGTGAGAGGAG
 801 GAGGAGAGGGAGAATTCTGTTCTCCCAGAGCTGCACCTGCCTCGCAGAGGCCAGCACCCCACTCTCCTGCCTCCAGTGGC
 881 CCTGCCGCAGATGTCTCCCAAAAAGTTGAGCCTTTCTAGATGGCTTAGGTGGCACCATGGCTCAGCAGGAGGGGCGGGAG
 961 GCACCAGGGTTCTTGTTTGGACCCTGCCCCTGGGCCATGGCCAGGTGACCATGGCTACATTGCCAAACCTCTGACTGCCA
1041 CAGCTGCAGACTGAGAGGGTGGGTCTGAGTCCCCACAATGTCTGAAGCTGCCCCTGGGATTCTCAGGCCAACCTGCCAAC
1121 AGCAAGCGGATTTTCTTGCAAGATCAGGGACCCCATTTCTGCAGCCAGTGTCTCCTGGGTGCCTTCTGAGGACTCCCACC
1201 CCCATCCCAGTATCTCATCTGTCCCCTCTCCTGGGGCTTAAGTGGGTTGCTTCCAGGCAGAAGCAGCCAAGGACCGATTC
1281 CAGGCACTTTCTGTAGCAAATGACTGTGAATTACGACTTCTCTTGCCCTTCTTCTAGCAGTCTGTGCCTCCTCTCTGACC
1361 AGTTTGGAGGGCACTGAAGAAAGGCAAGGGCCGTGCTGCTGCTGGGCGGGGCAGGAGAGGAGCCTGGCCAGTGTGCCACA
1441 TTAAATACCCGTGCAGGCGCGGAGAAGCAACCGGCACCCCCTTCCGGCCTGAAAGCCCTCCCTGCAAGAAGGTGTGCAGG
1521 AGAGAAGAGGCCCCGGCATGGGGATCTGGGTTCTAGAGGGCATGTGATGACTGTAAATGTTCACTGGGTGGGTAGGGAGT
1601 GGTATCCAGTGTTCAAGTGCAGAAATCTTTGGCTTTGCTACCAGTTCCATATGATGAGAAATAAACGTTCGCTGAGGTTT
1681 TGTTTCATAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ggaaAUAGUACU--UUGAUGAACu 5'
              | |||| |    ||||||| 
Target 5' cagaTTTCATTACCTCCTACTTGc 3'
 438 - 461 148.00 -7.30
2 
miRNA  3' ggaAAUAGUACUUUGAUG-AACu 5'
             | | ||||  :|||| ||| 
Target 5' aggTGACCATG--GCTACATTGc 3'
 1003 - 1023 123.00 -7.80
3 
miRNA  3' ggAAAUAGUACU------UUGAUGAACu 5'
            |||||: ||:      |  |:|||| 
Target 5' tcTTTATTTTGGTCCTTTA--TGCTTGa 3'
 741 - 766 109.00 -12.50
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN26636402 20 COSMIC
COSN30508915 24 COSMIC
COSN30485371 95 COSMIC
COSN30027538 131 COSMIC
COSN5740424 188 COSMIC
COSN24325465 571 COSMIC
COSN1102439 662 COSMIC
COSN20094622 662 COSMIC
COSN17181560 787 COSMIC
COSN29796172 1091 COSMIC
COSN28202125 1180 COSMIC
COSN27800418 1536 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs769395715 4 dbSNP
rs547652634 5 dbSNP
rs370925800 10 dbSNP
rs766661062 18 dbSNP
rs757483688 23 dbSNP
rs375396916 24 dbSNP
rs767831374 30 dbSNP
rs752886912 31 dbSNP
rs758274242 32 dbSNP
rs1179983876 35 dbSNP
rs1257212704 38 dbSNP
rs756219286 40 dbSNP
rs777781076 41 dbSNP
rs1187940130 43 dbSNP
rs1261574951 44 dbSNP
rs779852360 46 dbSNP
rs961534163 49 dbSNP
rs1168889678 51 dbSNP
rs992991731 53 dbSNP
rs917436163 57 dbSNP
rs949163716 60 dbSNP
rs980393843 62 dbSNP
rs1291423893 68 dbSNP
rs1288225559 72 dbSNP
rs926281645 77 dbSNP
rs1401645582 79 dbSNP
rs950275639 81 dbSNP
rs936351159 87 dbSNP
rs1344520352 90 dbSNP
rs140893776 92 dbSNP
rs1221299098 100 dbSNP
rs533051045 101 dbSNP
rs1248383828 103 dbSNP
rs1053668986 115 dbSNP
rs892382959 119 dbSNP
rs1466119284 126 dbSNP
rs1181555670 133 dbSNP
rs549585881 134 dbSNP
rs1264304457 141 dbSNP
rs1480268657 147 dbSNP
rs116173499 165 dbSNP
rs887362317 174 dbSNP
rs1427454250 180 dbSNP
rs945181719 182 dbSNP
rs1040938240 190 dbSNP
rs1383565290 196 dbSNP
rs1421413970 198 dbSNP
rs1298653347 212 dbSNP
rs190182009 226 dbSNP
rs1402812583 232 dbSNP
rs1294929594 244 dbSNP
rs1367817449 257 dbSNP
rs1163844029 260 dbSNP
rs901239945 262 dbSNP
rs1038462090 272 dbSNP
rs899562098 290 dbSNP
rs1242940450 296 dbSNP
rs1399455413 297 dbSNP
rs996962059 298 dbSNP
rs17163103 304 dbSNP
rs1029488419 308 dbSNP
rs1488919449 321 dbSNP
rs1176733916 325 dbSNP
rs1236429357 346 dbSNP
rs1441532615 348 dbSNP
rs890993831 356 dbSNP
rs1437679758 360 dbSNP
rs1009359325 363 dbSNP
rs781326789 369 dbSNP
rs566076103 370 dbSNP
rs1005974810 371 dbSNP
rs1378451835 372 dbSNP
rs1053101 374 dbSNP
rs534973944 381 dbSNP
rs1227015424 385 dbSNP
rs1015839231 386 dbSNP
rs1245075321 391 dbSNP
rs958806445 392 dbSNP
rs780090320 393 dbSNP
rs1347982529 398 dbSNP
rs767942260 402 dbSNP
rs1459149558 403 dbSNP
rs1242479090 404 dbSNP
rs992021258 405 dbSNP
rs1268643281 422 dbSNP
rs992941145 424 dbSNP
rs917341627 425 dbSNP
rs950161283 427 dbSNP
rs1168108623 428 dbSNP
rs1401937834 428 dbSNP
rs1201473660 441 dbSNP
rs1024459170 444 dbSNP
rs1335468929 446 dbSNP
rs557937669 447 dbSNP
rs982959288 450 dbSNP
rs1286385444 454 dbSNP
rs980467321 455 dbSNP
rs908769593 463 dbSNP
rs1214284153 468 dbSNP
rs1188582267 472 dbSNP
rs1239267695 474 dbSNP
rs1468707646 476 dbSNP
rs578074446 477 dbSNP
rs926367062 478 dbSNP
rs553697397 486 dbSNP
rs17357180 488 dbSNP
rs1413279693 496 dbSNP
rs989583981 500 dbSNP
rs913814134 512 dbSNP
rs754907589 531 dbSNP
rs1174649179 538 dbSNP
rs1191185402 543 dbSNP
rs1425392282 557 dbSNP
rs1477927457 558 dbSNP
rs1402028660 561 dbSNP
rs1053107 578 dbSNP
rs899530091 579 dbSNP
rs1040886324 582 dbSNP
rs1336723959 583 dbSNP
rs1360211605 589 dbSNP
rs1333651895 592 dbSNP
rs900976890 596 dbSNP
rs1408635216 614 dbSNP
rs747880796 615 dbSNP
rs1274396594 618 dbSNP
rs771914639 620 dbSNP
rs1221679205 626 dbSNP
rs574064287 627 dbSNP
rs543072164 635 dbSNP
rs1214234204 640 dbSNP
rs1201222466 644 dbSNP
rs1265060498 645 dbSNP
rs1280955259 645 dbSNP
rs1436318093 647 dbSNP
rs768412748 648 dbSNP
rs1280523042 649 dbSNP
rs1362832090 649 dbSNP
rs1419592845 649 dbSNP
rs1491045342 649 dbSNP
rs200339046 649 dbSNP
rs3831951 649 dbSNP
rs57658162 649 dbSNP
rs747816308 649 dbSNP
rs932416959 650 dbSNP
rs1051247950 652 dbSNP
rs1016036309 654 dbSNP
rs1197947074 654 dbSNP
rs897400989 657 dbSNP
rs1352987231 661 dbSNP
rs1491288762 661 dbSNP
rs1491518075 662 dbSNP
rs1375922998 663 dbSNP
rs1288007496 668 dbSNP
rs890884533 668 dbSNP
rs1009326623 672 dbSNP
rs1020764264 673 dbSNP
rs1264856268 674 dbSNP
rs1198999913 675 dbSNP
rs903764839 675 dbSNP
rs772984666 677 dbSNP
rs1231809352 679 dbSNP
rs1183331008 683 dbSNP
rs1439682721 686 dbSNP
rs980682820 692 dbSNP
rs1000768226 702 dbSNP
rs749723930 703 dbSNP
rs1420723256 706 dbSNP
rs1162984195 711 dbSNP
rs559774801 717 dbSNP
rs1033009242 721 dbSNP
rs1308102672 725 dbSNP
rs768845539 731 dbSNP
rs958063929 740 dbSNP
rs958915959 743 dbSNP
rs1376276943 753 dbSNP
rs528758172 775 dbSNP
rs1444571991 788 dbSNP
rs1311412835 792 dbSNP
rs1354269248 795 dbSNP
rs1403006342 795 dbSNP
rs147539738 796 dbSNP
rs1210048562 818 dbSNP
rs1256964642 824 dbSNP
rs1456069307 828 dbSNP
rs1024780468 832 dbSNP
rs971487857 833 dbSNP
rs1331645324 834 dbSNP
rs1240593067 837 dbSNP
rs1440821691 840 dbSNP
rs982929716 842 dbSNP
rs1235088120 844 dbSNP
rs541282292 845 dbSNP
rs374401856 854 dbSNP
rs907711358 857 dbSNP
rs974768663 858 dbSNP
rs1377608903 860 dbSNP
rs1395376682 862 dbSNP
rs532951137 865 dbSNP
rs1310385530 880 dbSNP
rs1204791621 886 dbSNP
rs548000962 887 dbSNP
rs932466823 888 dbSNP
rs1484098911 889 dbSNP
rs1315625880 891 dbSNP
rs932276035 893 dbSNP
rs1050796662 896 dbSNP
rs17163112 898 dbSNP
rs1200400995 904 dbSNP
rs563270105 913 dbSNP
rs941473752 919 dbSNP
rs1425959053 921 dbSNP
rs78094079 923 dbSNP
rs945153828 932 dbSNP
rs1042192253 934 dbSNP
rs371742047 935 dbSNP
rs1423244345 937 dbSNP
rs1476947612 938 dbSNP
rs1201372749 940 dbSNP
rs140159347 947 dbSNP
rs1014213694 954 dbSNP
rs1465557274 956 dbSNP
rs61785869 957 dbSNP
rs111667462 958 dbSNP
rs895073371 962 dbSNP
rs1396409768 963 dbSNP
rs1013307081 970 dbSNP
rs1024335131 974 dbSNP
rs1220074957 979 dbSNP
rs1278372493 981 dbSNP
rs971458428 984 dbSNP
rs1311109434 989 dbSNP
rs1230385697 995 dbSNP
rs1254791810 998 dbSNP
rs1004333735 1005 dbSNP
rs1484514688 1008 dbSNP
rs1033617001 1012 dbSNP
rs1262918263 1025 dbSNP
rs10494394 1026 dbSNP
rs1011043229 1028 dbSNP
rs534988120 1029 dbSNP
rs974277636 1030 dbSNP
rs1173982046 1036 dbSNP
rs921486869 1042 dbSNP
rs922510342 1051 dbSNP
rs954123023 1053 dbSNP
rs1358408309 1073 dbSNP
rs985300618 1092 dbSNP
rs551756930 1093 dbSNP
rs941223927 1096 dbSNP
rs764604300 1098 dbSNP
rs986856033 1099 dbSNP
rs1366414618 1100 dbSNP
rs571825905 1108 dbSNP
rs1298555598 1115 dbSNP
rs1302730935 1126 dbSNP
rs774585267 1128 dbSNP
rs762162724 1129 dbSNP
rs1285858652 1132 dbSNP
rs1351963936 1143 dbSNP
rs1042161298 1147 dbSNP
rs1242938329 1153 dbSNP
rs537279736 1162 dbSNP
rs906104160 1163 dbSNP
rs1489088852 1176 dbSNP
rs555973200 1184 dbSNP
rs1157979380 1188 dbSNP
rs925039870 1191 dbSNP
rs767788666 1193 dbSNP
rs1260055712 1195 dbSNP
rs1378362822 1198 dbSNP
rs1464762986 1199 dbSNP
rs1159540418 1215 dbSNP
rs1421311174 1215 dbSNP
rs557509413 1217 dbSNP
rs936472108 1225 dbSNP
rs1433850506 1226 dbSNP
rs376006959 1232 dbSNP
rs1367708235 1235 dbSNP
rs1054982889 1248 dbSNP
rs1189649315 1255 dbSNP
rs1391583400 1264 dbSNP
rs895034964 1266 dbSNP
rs182324466 1272 dbSNP
rs1442416002 1273 dbSNP
rs187347421 1276 dbSNP
rs1020709189 1277 dbSNP
rs1163380923 1281 dbSNP
rs113975812 1282 dbSNP
rs1459368723 1283 dbSNP
rs569672944 1284 dbSNP
rs1236338773 1288 dbSNP
rs148860823 1288 dbSNP
rs1177640903 1295 dbSNP
rs953753118 1296 dbSNP
rs570669317 1301 dbSNP
rs1004217844 1302 dbSNP
rs1322314975 1304 dbSNP
rs1464664933 1305 dbSNP
rs1367535387 1307 dbSNP
rs1394572012 1308 dbSNP
rs1398791665 1313 dbSNP
rs557803615 1313 dbSNP
rs1444983204 1315 dbSNP
rs116651280 1316 dbSNP
rs962840123 1326 dbSNP
rs1322453818 1327 dbSNP
rs1378910377 1328 dbSNP
rs1226926076 1333 dbSNP
rs1247584378 1335 dbSNP
rs1315135841 1345 dbSNP
rs996056604 1347 dbSNP
rs962829132 1352 dbSNP
rs770627412 1356 dbSNP
rs1028515243 1362 dbSNP
rs754750260 1379 dbSNP
rs954429775 1380 dbSNP
rs1205155973 1383 dbSNP
rs987048489 1385 dbSNP
rs142561602 1387 dbSNP
rs1311752615 1391 dbSNP
rs1250299950 1393 dbSNP
rs563486248 1394 dbSNP
rs1477273448 1405 dbSNP
rs1167998003 1406 dbSNP
rs150991248 1408 dbSNP
rs79516637 1409 dbSNP
rs1330187145 1411 dbSNP
rs558944390 1413 dbSNP
rs528042378 1419 dbSNP
rs190218064 1423 dbSNP
rs764898340 1427 dbSNP
rs1446345976 1431 dbSNP
rs1424998079 1432 dbSNP
rs1315144425 1436 dbSNP
rs936456476 1439 dbSNP
rs1218235914 1442 dbSNP
rs1276478073 1443 dbSNP
rs867924638 1445 dbSNP
rs1320098040 1449 dbSNP
rs754871867 1450 dbSNP
rs991046969 1451 dbSNP
rs79909517 1452 dbSNP
rs949325353 1459 dbSNP
rs778903106 1460 dbSNP
rs1046307375 1461 dbSNP
rs140993675 1462 dbSNP
rs1171460445 1471 dbSNP
rs1425133282 1473 dbSNP
rs115114623 1474 dbSNP
rs368021271 1477 dbSNP
rs569230896 1477 dbSNP
rs1376461617 1478 dbSNP
rs748011894 1479 dbSNP
rs898557553 1481 dbSNP
rs13886 1486 dbSNP
rs1017254992 1487 dbSNP
rs962695371 1495 dbSNP
rs550903949 1496 dbSNP
rs1397811308 1506 dbSNP
rs1352721375 1512 dbSNP
rs972519393 1513 dbSNP
rs1264947336 1517 dbSNP
rs1025458135 1525 dbSNP
rs1197629345 1528 dbSNP
rs971216795 1530 dbSNP
rs1478693155 1534 dbSNP
rs553961307 1535 dbSNP
rs1349691354 1536 dbSNP
rs7414413 1539 dbSNP
rs772695080 1539 dbSNP
rs1028903777 1544 dbSNP
rs890002078 1549 dbSNP
rs927401923 1554 dbSNP
rs1401499314 1563 dbSNP
rs777606655 1565 dbSNP
rs1329430251 1570 dbSNP
rs1443108497 1576 dbSNP
rs567814738 1580 dbSNP
rs1304099685 1581 dbSNP
rs1229624403 1587 dbSNP
rs1270404296 1591 dbSNP
rs990207001 1593 dbSNP
rs1310866055 1595 dbSNP
rs536766960 1599 dbSNP
rs914791220 1600 dbSNP
rs746689765 1601 dbSNP
rs553471091 1611 dbSNP
rs1042005701 1614 dbSNP
rs566956642 1615 dbSNP
rs966948997 1618 dbSNP
rs1193657741 1622 dbSNP
rs1254229900 1625 dbSNP
rs977707687 1628 dbSNP
rs1179299966 1630 dbSNP
rs1032034217 1633 dbSNP
rs1365898518 1637 dbSNP
rs770567194 1648 dbSNP
rs1162893307 1650 dbSNP
rs538687060 1653 dbSNP
rs1463124863 1657 dbSNP
rs1236653933 1660 dbSNP
rs916319018 1666 dbSNP
rs576986152 1667 dbSNP
rs558910693 1668 dbSNP
rs981955439 1671 dbSNP
rs745318486 1672 dbSNP
rs907770209 1689 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545216. RNA binding protein: AGO2. Condition:miR-124 transfection ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ggaaAUAGUACU--UUGAUGAACu 5'
              | |||| |    ||||||| 
Target 5' cagaUUUCAUUACCUCCUACUUGc 3'
 		3 - 26
Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 83667.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1 ...

- Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods.

Article - Kishore S; Jaskiewicz L; Burger L; Hausser et al.
- Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
Experimental Support 3 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HCT116
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in ERX177603. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_2_5 ...

- Krell J; Stebbing J; Carissimi C; Dabrowska et al., 2016, Genome research.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ggaaAUAGUACU--UUGAUGAACu 5'
              | |||| |    ||||||| 
Target 5' ---aUUUCAUUACCUCCUACUUG- 3'
 		1 - 20
Article - Krell J; Stebbing J; Carissimi C; Dabrowska et al.
- Genome research, 2016
DNA damage activates TP53-regulated surveillance mechanisms that are crucial in suppressing tumorigenesis. TP53 orchestrates these responses directly by transcriptionally modulating genes, including microRNAs (miRNAs), and by regulating miRNA biogenesis through interacting with the DROSHA complex. However, whether the association between miRNAs and AGO2 is regulated following DNA damage is not yet known. Here, we show that, following DNA damage, TP53 interacts with AGO2 to induce or reduce AGO2's association of a subset of miRNAs, including multiple let-7 family members. Furthermore, we show that specific mutations in TP53 decrease rather than increase the association of let-7 family miRNAs, reducing their activity without preventing TP53 from interacting with AGO2. This is consistent with the oncogenic properties of these mutants. Using AGO2 RIP-seq and PAR-CLIP-seq, we show that the DNA damage-induced increase in binding of let-7 family members to the RISC complex is functional. We unambiguously determine the global miRNA-mRNA interaction networks involved in the DNA damage response, validating them through the identification of miRNA-target chimeras formed by endogenous ligation reactions. We find that the target complementary region of the let-7 seed tends to have highly fixed positions and more variable ones. Additionally, we observe that miRNAs, whose cellular abundance or differential association with AGO2 is regulated by TP53, are involved in an intricate network of regulatory feedback and feedforward circuits. TP53-mediated regulation of AGO2-miRNA interaction represents a new mechanism of miRNA regulation in carcinogenesis.
LinkOut: [PMID: 26701625]
CLIP-seq Support 1 for dataset GSM545216
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / miR-124 transfection
Location of target site ENST00000253063.3 | 3UTR | UCCAGAUUUCAUUACCUCCUACUUGCCAUUCACCCAUCAAUGUGAAAGUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM714644
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repA
Location of target site ENST00000253063.3 | 3UTR | UCAUUUCCAGAUUUCAUUACCUCCUACUUGCCAUUCACCCAUCAAUGUGAAAGUCAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
139 hsa-miR-5697 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT055801 PLEKHA1 pleckstrin homology domain containing A1 2 12
MIRT066822 ZDHHC18 zinc finger DHHC-type containing 18 2 2
MIRT067331 SESN2 sestrin 2 2 4
MIRT072105 CHAC1 ChaC glutathione specific gamma-glutamylcyclotransferase 1 2 4
MIRT080621 PMAIP1 phorbol-12-myristate-13-acetate-induced protein 1 2 8
MIRT085213 SLC5A3 solute carrier family 5 member 3 2 4
MIRT086450 NABP1 nucleic acid binding protein 1 2 6
MIRT087916 TNRC6B trinucleotide repeat containing 6B 2 4
MIRT089461 TET3 tet methylcytosine dioxygenase 3 2 2
MIRT089610 MAT2A methionine adenosyltransferase 2A 2 10
MIRT107597 DNAJA1 DnaJ heat shock protein family (Hsp40) member A1 2 6
MIRT116036 DEPDC1 DEP domain containing 1 2 6
MIRT147331 KPNA2 karyopherin subunit alpha 2 2 10
MIRT206552 RHOQ ras homolog family member Q 2 2
MIRT211198 FGF2 fibroblast growth factor 2 2 6
MIRT215297 CREBRF CREB3 regulatory factor 2 2
MIRT227712 TBC1D13 TBC1 domain family member 13 2 2
MIRT243969 MAP1B microtubule associated protein 1B 2 2
MIRT250417 TNRC6A trinucleotide repeat containing 6A 2 6
MIRT294604 ZNF460 zinc finger protein 460 2 2
MIRT300122 STRADB STE20-related kinase adaptor beta 2 2
MIRT354709 SLC25A36 solute carrier family 25 member 36 2 2
MIRT442368 ZC3HAV1L zinc finger CCCH-type containing, antiviral 1 like 2 2
MIRT443617 AVPR1A arginine vasopressin receptor 1A 2 2
MIRT444711 ZNF772 zinc finger protein 772 2 2
MIRT447556 C14orf37 chromosome 14 open reading frame 37 2 2
MIRT448685 MAPK6 mitogen-activated protein kinase 6 2 2
MIRT450817 LIFR LIF receptor alpha 2 2
MIRT451368 UQCR11 ubiquinol-cytochrome c reductase, complex III subunit XI 2 2
MIRT457302 ZBTB45 zinc finger and BTB domain containing 45 2 2
MIRT458779 CES2 carboxylesterase 2 2 2
MIRT458864 CD55 CD55 molecule (Cromer blood group) 2 2
MIRT460276 SLC26A2 solute carrier family 26 member 2 2 2
MIRT461711 ZNF426 zinc finger protein 426 2 2
MIRT466690 TAF13 TATA-box binding protein associated factor 13 2 4
MIRT468811 SACS sacsin molecular chaperone 2 10
MIRT476013 GTF2A1 general transcription factor IIA subunit 1 2 8
MIRT477764 EDEM3 ER degradation enhancing alpha-mannosidase like protein 3 2 2
MIRT478541 CTNS cystinosin, lysosomal cystine transporter 2 2
MIRT479230 CKS2 CDC28 protein kinase regulatory subunit 2 2 2
MIRT479980 CARD10 caspase recruitment domain family member 10 2 2
MIRT480800 BLOC1S2 biogenesis of lysosomal organelles complex 1 subunit 2 2 6
MIRT482442 ADM adrenomedullin 2 10
MIRT487566 LOXL2 lysyl oxidase like 2 2 2
MIRT499512 RNF6 ring finger protein 6 2 6
MIRT501861 MTDH metadherin 2 2
MIRT502460 FRAT2 FRAT2, WNT signaling pathway regulator 2 6
MIRT502704 CREBZF CREB/ATF bZIP transcription factor 2 6
MIRT502856 CHEK2 checkpoint kinase 2 2 6
MIRT503188 ADAM17 ADAM metallopeptidase domain 17 2 2
MIRT503528 ATG12 autophagy related 12 2 2
MIRT505087 ZBTB18 zinc finger and BTB domain containing 18 2 6
MIRT507490 E2F7 E2F transcription factor 7 2 6
MIRT518010 RPL4 ribosomal protein L4 2 4
MIRT520767 TCF23 transcription factor 23 2 2
MIRT524551 CCND2 cyclin D2 2 8
MIRT527916 B3GALT5 beta-1,3-galactosyltransferase 5 2 2
MIRT530025 KIAA1875 WD repeat domain 97 2 2
MIRT532306 HAUS3 HAUS augmin like complex subunit 3 2 2
MIRT532898 ZNF451 zinc finger protein 451 2 2
MIRT533591 TOB1 transducer of ERBB2, 1 2 6
MIRT534161 SLC9A7 solute carrier family 9 member A7 2 2
MIRT539776 KIAA0408 KIAA0408 2 6
MIRT541045 SOGA3 SOGA family member 3 2 6
MIRT544310 VANGL2 VANGL planar cell polarity protein 2 2 4
MIRT545812 ZNF608 zinc finger protein 608 2 2
MIRT546436 SNX5 sorting nexin 5 2 2
MIRT547715 KLHL15 kelch like family member 15 2 4
MIRT549105 C16orf70 chromosome 16 open reading frame 70 2 4
MIRT551192 EPB41L3 erythrocyte membrane protein band 4.1 like 3 2 2
MIRT551671 RPL9 ribosomal protein L9 2 4
MIRT552050 ALG1 ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase 2 2
MIRT553113 UBR3 ubiquitin protein ligase E3 component n-recognin 3 (putative) 2 2
MIRT553329 TSC22D2 TSC22 domain family member 2 2 2
MIRT554371 SFPQ splicing factor proline and glutamine rich 2 2
MIRT555745 PDCD10 programmed cell death 10 2 2
MIRT556351 MAN2A1 mannosidase alpha class 2A member 1 2 2
MIRT557461 GSK3B glycogen synthase kinase 3 beta 2 4
MIRT558082 EPC2 enhancer of polycomb homolog 2 2 2
MIRT558501 CYP26B1 cytochrome P450 family 26 subfamily B member 1 2 4
MIRT560288 HRH2 histamine receptor H2 2 2
MIRT561124 ATP2B1 ATPase plasma membrane Ca2+ transporting 1 2 2
MIRT562629 ARNTL2 aryl hydrocarbon receptor nuclear translocator like 2 2 2
MIRT564087 NSA2 NSA2, ribosome biogenesis homolog 2 2
MIRT564209 SDE2 SDE2 telomere maintenance homolog 2 2
MIRT564327 CCNT1 cyclin T1 2 2
MIRT564996 WNK1 WNK lysine deficient protein kinase 1 2 2
MIRT565212 TROVE2 TROVE domain family member 2 2 2
MIRT568261 BMP2K BMP2 inducible kinase 2 2
MIRT571352 RPL37 ribosomal protein L37 2 4
MIRT574715 HAUS8 HAUS augmin like complex subunit 8 2 2
MIRT610600 NUS1 NUS1 dehydrodolichyl diphosphate synthase subunit 2 2
MIRT615444 FAXC failed axon connections homolog 2 2
MIRT618242 PGBD4 piggyBac transposable element derived 4 2 2
MIRT618694 CAMK1D calcium/calmodulin dependent protein kinase ID 2 2
MIRT620289 GLTP glycolipid transfer protein 2 2
MIRT621242 SIGLEC9 sialic acid binding Ig like lectin 9 2 4
MIRT626894 ANGEL1 angel homolog 1 2 2
MIRT631347 LATS1 large tumor suppressor kinase 1 2 2
MIRT631742 NKX2-1 NK2 homeobox 1 2 2
MIRT633819 WDR92 WD repeat domain 92 2 2
MIRT648370 CYB5A cytochrome b5 type A 2 2
MIRT648892 TUBGCP5 tubulin gamma complex associated protein 5 2 2
MIRT653570 SLC35B3 solute carrier family 35 member B3 2 2
MIRT654652 PTAFR platelet activating factor receptor 2 2
MIRT657173 INTU inturned planar cell polarity protein 2 2
MIRT658874 DSTYK dual serine/threonine and tyrosine protein kinase 2 2
MIRT666654 RBM48 RNA binding motif protein 48 2 2
MIRT669273 C1orf50 chromosome 1 open reading frame 50 2 2
MIRT671790 RGS17 regulator of G protein signaling 17 2 2
MIRT673619 HPSE heparanase 2 4
MIRT676182 HIST1H2BJ histone cluster 1 H2B family member j 2 2
MIRT676317 GTF2H2C GTF2H2 family member C 2 2
MIRT676661 LRRC27 leucine rich repeat containing 27 2 2
MIRT676678 ICA1L islet cell autoantigen 1 like 2 2
MIRT677497 GTF2H2 general transcription factor IIH subunit 2 2 2
MIRT678021 SPIC Spi-C transcription factor 2 2
MIRT678030 SLC1A5 solute carrier family 1 member 5 2 2
MIRT678363 PPAP2B phospholipid phosphatase 3 2 2
MIRT678438 PDE4C phosphodiesterase 4C 2 2
MIRT678497 ARHGEF39 Rho guanine nucleotide exchange factor 39 2 2
MIRT678648 PDCD4 programmed cell death 4 2 2
MIRT679089 PURB purine rich element binding protein B 2 2
MIRT679121 NMNAT1 nicotinamide nucleotide adenylyltransferase 1 2 2
MIRT680087 THAP1 THAP domain containing 1 2 2
MIRT684600 ORAI2 ORAI calcium release-activated calcium modulator 2 2 2
MIRT686267 YTHDF1 YTH N6-methyladenosine RNA binding protein 1 2 2
MIRT687836 ISG20L2 interferon stimulated exonuclease gene 20 like 2 2 2
MIRT688028 GPN2 GPN-loop GTPase 2 2 2
MIRT697864 UBE2H ubiquitin conjugating enzyme E2 H 2 2
MIRT698304 TMEM2 transmembrane protein 2 2 2
MIRT701714 MTMR12 myotubularin related protein 12 2 2
MIRT703736 FAM126B family with sequence similarity 126 member B 2 2
MIRT705971 ACBD5 acyl-CoA binding domain containing 5 2 2
MIRT714346 DIP2A disco interacting protein 2 homolog A 2 2
MIRT714614 EXO5 exonuclease 5 2 2
MIRT722607 CCDC152 coiled-coil domain containing 152 2 2
MIRT722876 XPNPEP3 X-prolyl aminopeptidase 3 2 2
MIRT724406 WWC1 WW and C2 domain containing 1 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-5697 Cisplatin 5460033 NSC119875 approved resistant High Ovarian Cancer cell line (A2780CP20)
hsa-mir-5697 Cisplatin 5460033 NSC119875 approved resistant cell line (CIS)
hsa-miR-5697 Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-5697 Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-5697 Cisplatin 5460033 NSC119875 approved resistant cell line (CP20)
hsa-miR-5697 Paclitaxel 36314 NSC125973 approved sensitive cell line (A2780)
hsa-miR-5697 Cisplatin 5460033 NSC119875 approved sensitive cell line (A2780)

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