pre-miRNA Information | |
---|---|
pre-miRNA | hsa-mir-4666a |
Genomic Coordinates | chr1: 228462074 - 228462152 |
Description | Homo sapiens miR-4666a stem-loop |
Comment | None |
RNA Secondary Structure |
Mature miRNA Information | ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mature miRNA | hsa-miR-4666a-5p | |||||||||||||||||||||||||||
Sequence | 10| AUACAUGUCAGAUUGUAUGCC |30 | |||||||||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||||||||
Experiments | Illumina | |||||||||||||||||||||||||||
SNPs in miRNA |
|
|||||||||||||||||||||||||||
Putative Targets |
Gene Information | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | DNAJB4 | ||||||||||||||||||||
Synonyms | DNAJW, DjB4, HLJ1 | ||||||||||||||||||||
Description | DnaJ heat shock protein family (Hsp40) member B4 | ||||||||||||||||||||
Transcript | NM_007034 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on DNAJB4 | |||||||||||||||||||||
3'UTR of DNAJB4 (miRNA target sites are highlighted) |
>DNAJB4|NM_007034|3'UTR 1 AATGAAGAACTTTGTTACACATATTTTGATAAGGCACTGAAAATATAAAAGGACTGGTAGTTTACTGATGTAGATGTGAA 81 TTCTGTATAAAGATGTGTAAATTCTTTTGAGGGTTCATTAAATTGCATGAATAGAGACGGGTCAAATAAATAGGCAAAAG 161 GGATTTTTACAGTTAGAGATAAAAGAGAAAACCATTCACTGTATTTTATTTCATTTCTCCTGATTCAGATATTTTTAGTA 241 ATTTGCTTATATGTAAAAGTTGTTTTTGTGGAGTCAGTGGATATATTTCTAATGAAGTGCTAGACTATCCAATTACTTAA 321 TTTCTTATACCTTTAGATAATCAGTATGAAAAGTTCCCATTTATAATGGAAATGAAAATTCTTAACTAAACTATACATGT 401 AATATGTATTTCTAGAAGAGAATAAAAACCCAAGTCAGTTATTAGATTTAAATCACCTTCTGAAATGCTGCTATAGGGCT 481 GGTATCTGTAAAAGAATATCCTGATGCATCTGTTTCACCATTTTGATTTTTAAAGTATGCTGTAGCATTTCTTAATAACA 561 TCGTTGTGATGTTCTTAAGGCAGATCTTTCTTCATAAAAAGGAAAGTAATGGCAATTTCTCTCCTGTGGAAATCCCAATT 641 GCTTGAATTACTGATATTTTAGAATAGACTTTTTAAAATGCCATATGTAATTTTATGCAAGTTGACTATATATCTTGTAC 721 TTAATAAATTATAGGCTCATTTTGTTCTCTGCTAGTTTAAAGTAATTCGTTTAATAATAGATGTGTTTTTAGAGGAAATG 801 CTGTTACTTGGAATTAATTTTCCAGTTATACAGTCTTCTATAACTTACTAATAATATTCTATATGTACTTTATGTAATTT 881 CCCTAAAAAGAATGAACTACCACTACACTATGGTGTTAAACCAAAATATAGGGAAAATAAACACTAACTGCTGCTTATGG 961 ATAATGTTGCAACTACTTGTTATGCATATAAATATTTTACTTTTTCACATGTATAGATTGCATTTCTTAGGTGTTTTAAT 1041 TTTTTAAATATATTTATGTTTTAAAAATTTAGTTTTGTTTTCTGTTTTATAACTATAGTGAGAATGATGTTTTGAAGCAA 1121 AATTTTTGGTTATAAAATAGTTTTCAGGATTATATATATATATACTGGATCCTATCGCCTTTTAGTAGAATATGAAATAT 1201 TCTTTTAGAAATCCAATATAAATAGGTTATAATAGCCATATTCTTTATTACTTTATTGAGATATAATTTACATGCCATAA 1281 AGTTTACCCTTAAAATATATAATTCAGTGGTTTTTAGTGATATTTACAAAGTGGTACAATCATCATCACTTTCTAATTCC 1361 AGAATATTTTCATCACCCCAAAAAGAAATCCTAAATCCATTAGCAGTTACTTCCCATTCTTCCCTTCCTCCAGCCCCTGA 1441 AAACACTACCATCTACTTTTCATCTGTATGGATTTGTCTGTTCTGGACATTTCACATAAATAGACTCAAACAATATATGG 1521 CTTTTTTTTTTTTTGGTCTGGCCTCTTTCATTTAGCTTAATGTTTTCAAGGTTCATCTATGTTGTATCACGTATCAGTAC 1601 TTTATTTTTTGTGTGGCACGTCATATGGATATACCACAATTTGTTTATCTTTTCATTAATTATGGGCATTTGGCTTATTT 1681 CTACTTTTTTGGCTATTATAAATAATGCTGCTGTGAAAAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
||||||||||||||||||||
miRNA-target interactions (Predicted by miRanda) |
|
||||||||||||||||||||
DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |
---|---|
miRNA:Target | ---- |
Validation Method |
|
Conditions | HEK293 |
Disease | 11080.0 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
"PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1
... - Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods. |
Article |
- Kishore S; Jaskiewicz L; Burger L; Hausser et al. - Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
|
CLIP-seq Support 1 for dataset GSM714644 | |
---|---|
Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | HEK293 / completeT1, repA |
Location of target site | ENST00000370763.5 | 3UTR | CAUAUAAAUAUUUUACUUUUUCACAUG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21572407 / GSE28865 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
---|---|---|---|---|---|---|---|
|
MiRNA-Target Expression Profile (TCGA) | |||||||
---|---|---|---|---|---|---|---|
|
83 hsa-miR-4666a-5p Target Genes:
Functional analysis:
ID | Target | Description | Validation methods | |||||||||
Strong evidence | Less strong evidence | |||||||||||
MIRT057266 | FAM35A | family with sequence similarity 35 member A | 2 | 2 | ||||||||
MIRT059969 | PATL1 | PAT1 homolog 1, processing body mRNA decay factor | 2 | 6 | ||||||||
MIRT079031 | TNRC6C | trinucleotide repeat containing 6C | 2 | 2 | ||||||||
MIRT079631 | DNAJB4 | DnaJ heat shock protein family (Hsp40) member B4 | 2 | 2 | ||||||||
MIRT086974 | LANCL1 | LanC like 1 | 2 | 2 | ||||||||
MIRT091804 | GOLGA4 | golgin A4 | 2 | 2 | ||||||||
MIRT229501 | EIF1AX | eukaryotic translation initiation factor 1A, X-linked | 2 | 4 | ||||||||
MIRT255972 | WDR17 | WD repeat domain 17 | 2 | 2 | ||||||||
MIRT262975 | ADO | 2-aminoethanethiol dioxygenase | 2 | 2 | ||||||||
MIRT264774 | PAFAH1B2 | platelet activating factor acetylhydrolase 1b catalytic subunit 2 | 2 | 2 | ||||||||
MIRT334169 | CCND1 | cyclin D1 | 2 | 6 | ||||||||
MIRT345868 | SRSF2 | serine and arginine rich splicing factor 2 | 2 | 2 | ||||||||
MIRT452477 | DDX4 | DEAD-box helicase 4 | 2 | 2 | ||||||||
MIRT455764 | TSPAN6 | tetraspanin 6 | 2 | 4 | ||||||||
MIRT461627 | DCAF15 | DDB1 and CUL4 associated factor 15 | 2 | 4 | ||||||||
MIRT465169 | TRPV2 | transient receptor potential cation channel subfamily V member 2 | 2 | 4 | ||||||||
MIRT468950 | RPS14 | ribosomal protein S14 | 2 | 6 | ||||||||
MIRT483236 | C2orf72 | chromosome 2 open reading frame 72 | 2 | 8 | ||||||||
MIRT498544 | TMEM30B | transmembrane protein 30B | 2 | 2 | ||||||||
MIRT500633 | TXNIP | thioredoxin interacting protein | 2 | 4 | ||||||||
MIRT504113 | GPR158 | G protein-coupled receptor 158 | 2 | 2 | ||||||||
MIRT504428 | ZNF85 | zinc finger protein 85 | 2 | 6 | ||||||||
MIRT505036 | ZNF451 | zinc finger protein 451 | 2 | 2 | ||||||||
MIRT506526 | MRPL17 | mitochondrial ribosomal protein L17 | 2 | 6 | ||||||||
MIRT508773 | GSG1 | germ cell associated 1 | 2 | 2 | ||||||||
MIRT517297 | ELF4 | E74 like ETS transcription factor 4 | 2 | 6 | ||||||||
MIRT519353 | OBFC1 | STN1, CST complex subunit | 2 | 4 | ||||||||
MIRT523891 | ENPP6 | ectonucleotide pyrophosphatase/phosphodiesterase 6 | 2 | 6 | ||||||||
MIRT527509 | ZNF134 | zinc finger protein 134 | 2 | 2 | ||||||||
MIRT531218 | IFNGR2 | interferon gamma receptor 2 | 2 | 2 | ||||||||
MIRT535957 | MOGAT1 | monoacylglycerol O-acyltransferase 1 | 2 | 2 | ||||||||
MIRT537229 | GALNT7 | polypeptide N-acetylgalactosaminyltransferase 7 | 2 | 4 | ||||||||
MIRT537337 | FKBP5 | FK506 binding protein 5 | 2 | 2 | ||||||||
MIRT539125 | ARHGEF17 | Rho guanine nucleotide exchange factor 17 | 2 | 2 | ||||||||
MIRT539910 | ISPD | isoprenoid synthase domain containing | 2 | 2 | ||||||||
MIRT541527 | MGAT4C | MGAT4 family member C | 2 | 2 | ||||||||
MIRT546119 | USP25 | ubiquitin specific peptidase 25 | 2 | 2 | ||||||||
MIRT548846 | CERCAM | cerebral endothelial cell adhesion molecule | 2 | 2 | ||||||||
MIRT549892 | LINC00955 | long intergenic non-protein coding RNA 955 | 2 | 2 | ||||||||
MIRT549898 | ADH4 | alcohol dehydrogenase 4 (class II), pi polypeptide | 2 | 2 | ||||||||
MIRT550763 | ENOX2 | ecto-NOX disulfide-thiol exchanger 2 | 2 | 4 | ||||||||
MIRT553200 | UBE2A | ubiquitin conjugating enzyme E2 A | 2 | 2 | ||||||||
MIRT553970 | SRSF10 | serine and arginine rich splicing factor 10 | 2 | 2 | ||||||||
MIRT554092 | SMU1 | DNA replication regulator and spliceosomal factor | 2 | 2 | ||||||||
MIRT555208 | PROX1 | prospero homeobox 1 | 2 | 4 | ||||||||
MIRT555834 | PAX5 | paired box 5 | 2 | 4 | ||||||||
MIRT556865 | JAZF1 | JAZF zinc finger 1 | 2 | 2 | ||||||||
MIRT558594 | CREBL2 | cAMP responsive element binding protein like 2 | 2 | 2 | ||||||||
MIRT559690 | AGO2 | argonaute 2, RISC catalytic component | 2 | 4 | ||||||||
MIRT563312 | ORC4 | origin recognition complex subunit 4 | 2 | 2 | ||||||||
MIRT563585 | FAM229B | family with sequence similarity 229 member B | 2 | 2 | ||||||||
MIRT563853 | ALYREF | Aly/REF export factor | 2 | 4 | ||||||||
MIRT565146 | TUBB2A | tubulin beta 2A class IIa | 2 | 2 | ||||||||
MIRT565769 | SEPHS1 | selenophosphate synthetase 1 | 2 | 2 | ||||||||
MIRT568317 | BACH1 | BTB domain and CNC homolog 1 | 2 | 2 | ||||||||
MIRT575387 | Unc5b | unc-5 netrin receptor B | 2 | 4 | ||||||||
MIRT607728 | BDH1 | 3-hydroxybutyrate dehydrogenase 1 | 2 | 8 | ||||||||
MIRT612691 | PLXNA4 | plexin A4 | 2 | 4 | ||||||||
MIRT615916 | GDPD1 | glycerophosphodiester phosphodiesterase domain containing 1 | 2 | 2 | ||||||||
MIRT629792 | P2RY1 | purinergic receptor P2Y1 | 2 | 2 | ||||||||
MIRT632119 | FKBP9 | FK506 binding protein 9 | 2 | 2 | ||||||||
MIRT645554 | ZDHHC15 | zinc finger DHHC-type containing 15 | 2 | 4 | ||||||||
MIRT651479 | WWC3 | WWC family member 3 | 2 | 2 | ||||||||
MIRT654138 | RPH3A | rabphilin 3A | 2 | 6 | ||||||||
MIRT655191 | PHAX | phosphorylated adaptor for RNA export | 2 | 2 | ||||||||
MIRT665543 | UNC5B | unc-5 netrin receptor B | 2 | 5 | ||||||||
MIRT668057 | GRIK3 | glutamate ionotropic receptor kainate type subunit 3 | 2 | 2 | ||||||||
MIRT678978 | CERS4 | ceramide synthase 4 | 2 | 4 | ||||||||
MIRT679124 | RBM3 | RNA binding motif (RNP1, RRM) protein 3 | 2 | 2 | ||||||||
MIRT686776 | AZF1 | azoospermia factor 1 | 2 | 2 | ||||||||
MIRT687144 | PTPN12 | protein tyrosine phosphatase, non-receptor type 12 | 2 | 2 | ||||||||
MIRT691453 | C21orf58 | chromosome 21 open reading frame 58 | 2 | 2 | ||||||||
MIRT691469 | FAM98B | family with sequence similarity 98 member B | 2 | 2 | ||||||||
MIRT697031 | UHRF1BP1 | UHRF1 binding protein 1 | 2 | 2 | ||||||||
MIRT698401 | TM9SF3 | transmembrane 9 superfamily member 3 | 2 | 2 | ||||||||
MIRT702056 | RNMT | RNA guanine-7 methyltransferase | 2 | 2 | ||||||||
MIRT705903 | ADAM9 | ADAM metallopeptidase domain 9 | 2 | 2 | ||||||||
MIRT707491 | MMADHC | methylmalonic aciduria and homocystinuria, cblD type | 2 | 2 | ||||||||
MIRT708080 | KLHL23 | kelch like family member 23 | 2 | 2 | ||||||||
MIRT709738 | TRIM27 | tripartite motif containing 27 | 2 | 2 | ||||||||
MIRT710056 | RWDD2A | RWD domain containing 2A | 2 | 2 | ||||||||
MIRT710226 | KCNK1 | potassium two pore domain channel subfamily K member 1 | 2 | 2 | ||||||||
MIRT712043 | STYK1 | serine/threonine/tyrosine kinase 1 | 2 | 2 |