pre-miRNA Information | |
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pre-miRNA | hsa-mir-3622a |
Genomic Coordinates | chr8: 27701677 - 27701759 |
Description | Homo sapiens miR-3622a stem-loop |
Comment | None |
RNA Secondary Structure |
Mature miRNA Information | |||||||||||||||||||||||||||||
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Mature miRNA | hsa-miR-3622a-3p | ||||||||||||||||||||||||||||
Sequence | 50| UCACCUGACCUCCCAUGCCUGU |71 | ||||||||||||||||||||||||||||
Evidence | Experimental | ||||||||||||||||||||||||||||
Experiments | Illumina | ||||||||||||||||||||||||||||
Editing Events in miRNAs |
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SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | PRKACB | ||||||||||||||||||||
Synonyms | PKA C-beta, PKACB | ||||||||||||||||||||
Description | protein kinase cAMP-activated catalytic subunit beta | ||||||||||||||||||||
Transcript | NM_002731 | ||||||||||||||||||||
Other Transcripts | NM_182948 , NM_207578 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on PRKACB | |||||||||||||||||||||
3'UTR of PRKACB (miRNA target sites are highlighted) |
>PRKACB|NM_002731|3'UTR 1 AGAGGAACAAGATGACATCTGAGCTCACACTCAGTGTTTGCACTCTGTTGAGAGATAAGGTAGAGCTGAGACCGTCCTTG 81 TTGAAGCAGTTACCTAGTTCCTTCATTCCAACGACTGAGTGAGGTCTTTATTGCCATCATCCCGTGTGCGCACTCTGCAT 161 CCACCTATGTAACAAGGCACCGCTAAGCAAGCATTGTCTGTGCCATAACACAGTACTAGACCACTTTCTTACTTCTCTTT 241 GGGTTGTCTTTCTCCTCTCCTATATCCATTTCTTCCTTTTCCAATTTCATTGGTTTTCTCTAAACAGTGCTCCATTTTAT 321 TTTGTTGGTGTTTCAGATGGGCAGTGTTATGGCTACGTGATATTTGAAGGGAAGGATAAGTGTTGCTTTCAGTAGTTATT 401 GCCAATATTGTTGTTGGTCAATGGCTTGAAGATAAACTTTCTAATAATTATTATTTCTTTGAGTAGCTCAGACTTGGTTT 481 TGCCAAAACTCTTGGTAATTTTTGAAGATAGACTGTCTTATCACCAAGGAAATTTATACAAATTAAGACTAACTTTCTTG 561 GAATTCACTATTCTGGCAATAAATTTTGGTAGACTAATACAGTACAGCTAGACCCAGAAATTTGGAAGGCTGTAGATCAG 641 AGGTTCTAGTTCCCTTTCCCTCCTTTTATATCCTCCTCTCCTTGAGTAATGAAGTGACCAGCCTGTGTAGTGTGACAAAC 721 GTGTCTCATTCAGCAGGAAAAACTAATGATATGGATCATCACCCAGATTCTCTCACTTGGTACCAGCATTTCTGTAGGTA 801 TTAGAGAAGAGTTCTAAGTTTTCTAAACCTTAACTGTTCCTTAAGGATTTTAGCCAGTATTTTAATAGAACATGATTAAT 881 GAAAGTGACAAATTTTAAATTTTCTCTAATAGTCCTCATCATAAACTTTTTAAAGGAAAATAAGCAAACTAAAAAGAACA 961 TTGGTTTAGATAAATACTTATACTTTGCAAAGTCAAAAATGGCTTGATTTTTGGAAACAATATAGAGGTATTCATATTTA 1041 AATGAGGGTTTACATTTGTTTTGTTTTGTAACCGTTAAAAAGAAGTTGTTTCCAGCTAATTATTGTGGTGTACTATATTT 1121 GTGAGCCTAGGGTAGGGGCACTGCTGCAACTTCTGCTTTCATCCCATGCCTCATCAATGAGGAAAGGGAACAAAGTGTAT 1201 AAAACTGCCACAATTGTATTTTAATTTTGAGGTATGATATTTTCAGATATTTCATAATTTCTAACCTCTGTTCTCTCAGT 1281 AAACAGAATGTCTGATCGATCATGCAGATACAATGTTGGTATTTGAGAGGTTAGTTTTTTTCCTACACTTTTTTTTGCCA 1361 ACTGACTTAACAACATTGCTGTCAGGTGGAAATTTCAAGCACTTTTGCACATTTAGTTCAGTGTTTGTTGAGAATCCATG 1441 GCTTAACCCACTTGTTTTGCTATTTTTTTCTTTGCTTTTAATTTTCCCCATCTGATTTTATCTCTGCGTTTCAGTGACCT 1521 ACCTTAAAACAACACACGAGAAGAGTTAAACTGGGTTCATTTTAATGATCAATTTACCTGCATATAAAATTTATTTTTAA 1601 TCAAGCTGATCTTAATGTATATAATCATTCTATTTGCTTTATTATCGGTGCAGGTAGGTCATTAACACCACTTCTTTTCA 1681 TCTGTACCACACCCTGGTGAAACCTTTGAAGACATAAAAAAAACCTGTCTGAGATGTTCTTTCTACCAATCTATATGTCT 1761 TTCGGTTATCAAGTGTTTCTGCATGGTAATGTCATGTAAATGCTGATATTGATTTCACTGGTCCATCTATATTTAAAACG 1841 TGCAAGAAAAAAATAAAATACTCTGCTCTAGCAAGTTTTGTGTAACAAAGGCATATCGTCATGTTAATAAATTTAAAACA 1921 TCATTCGTATAAAATATTTTAATTTTCTTGTATTTCATTTAGACCCAAGAACATGCTGACCAATGTGTTCTATATGTAAA 2001 CTACAAATTCTATGGTAGCTTTGTTGTATATTATTGTAAAATTATTTTAATAAGTCATGGGGATGACAATTTGATTATTA 2081 CAATTTAGTTTTCAGTAATCAAAAAGATTTCTATGAATTCTAAAAAATATTTTTTTCTATGAAATTACTAGTGCCCAGCT 2161 GTAGAATCTACCTTAGGTAGATGATCCCTAGACATACGTTGGTTTTGAGGGCTATTCAGCCATTCCATTTTACTCTCTAT 2241 TTAAAGGCCGTGAGCAAGCTTGTCATGAGCAAATATGTCAAGGGAGTCAATTTCTGACCAATCAAGTACACTAAATTAGA 2321 ATATTTTTAAAGTATGTAACATTCCCAGTTTCAGCCACAATTTAGCCAAGAATAAGATAAAAACTTGAATAAGAAGTAAG 2401 TAGCATAAATCAGTATTTAACCTAAAATTACATATTTGAAACAGAAGATATTATGTTATGCTCAGTAAATAATTAAGAGA 2481 TGGCATTGTGTAAGAAGGAGCCCTAGACTGAAAGTCAAGACATCTGAATTTCAGGCTGGAAAACTATCAGTATGATCTCA 2561 GCCTCAGTTCTCTTGTCTGTAAAATGGAAGAACTGGATTAGGCAGTTTGTAAGATTCCTCCTAACTTTCACAGTCGATGA 2641 CAAGATTGTCTTTTTATCTGATATTTTGAAGGGTATATTGCTTTGAAGTAAGTCTCAATAAGGCAATATATTTTAGGGCA 2721 TCTTTCTTCTTATCTCTGACAGTGTTCTTAAAATTATTTGAATATCATAAGAGCCTTGGTGTCTGTCCTAATTCCTTTCT 2801 CACTCACCGATGCTGAATACCCAGTTGAATCAAACTGTCAACCTACCAAAAACGATATTGTGGCTTATGGGTATTGCTGT 2881 CTCATTCTTGGTATATTCTTGTGTTAACTGCCCATTGGCCTGAAAATACTCATTGTAAGCCTGAAAAAAAAAATCTTTCC 2961 CACTGTTTTTTCTGCTTGTTGTAAGAATCAAATGAAATAATGTATGTGAAAGCACCTTGTAAACTGTAACCTATCAATGT 3041 AAAATGTTAAGGTGTGTTGTTATTTCATTAATTACTTCTTTGTTTAGAATGGAATTTCCTATGCACTACTGTAGCTAGGA 3121 AATGCTGAAAACAACTGTGTTTTTTAATTAATCAATAACTGCAAAATTAAAGTACCTTCAATGGATAAGACAAAAAAAAA 3201 AAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | HEK293 | ||||||
Disease | 5567.0 | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
"PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1
... - Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Kishore S; Jaskiewicz L; Burger L; Hausser et al. - Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
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CLIP-seq Support 1 for dataset GSM4903833 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / CTL_TD_21_a |
Location of target site | NM_001242857 | 3UTR | AGGUGGAAAUUUCAAGCACUUUUGCACAUUUAGUUCAGUGUUUGUUGAGAAUCCAUGGCUUAACCCACUUGUUUUGCUAUUUUUUUCUUUGCUUUUAAUUUUCCCCAUCUGAUUUUAUCUCUGC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM714644 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | HEK293 / completeT1, repA |
Location of target site | ENST00000370689.2 | 3UTR | UCAGGUGGAAAUUUCAAGCACUUUUGCACAUUUAG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21572407 / GSE28865 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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104 hsa-miR-3622a-3p Target Genes:
Functional analysis:
ID | Target | Description | Validation methods | |||||||||
Strong evidence | Less strong evidence | |||||||||||
MIRT052866 | MRPL16 | mitochondrial ribosomal protein L16 | 1 | 1 | ||||||||
MIRT076712 | NUFIP2 | NUFIP2, FMR1 interacting protein 2 | 2 | 2 | ||||||||
MIRT080211 | PRKACB | protein kinase cAMP-activated catalytic subunit beta | 2 | 2 | ||||||||
MIRT081397 | GTPBP3 | GTP binding protein 3, mitochondrial | 2 | 2 | ||||||||
MIRT107157 | ZBTB43 | zinc finger and BTB domain containing 43 | 2 | 2 | ||||||||
MIRT254070 | BACH1 | BTB domain and CNC homolog 1 | 2 | 2 | ||||||||
MIRT409789 | FOXO3 | forkhead box O3 | 2 | 2 | ||||||||
MIRT448680 | MAPK9 | mitogen-activated protein kinase 9 | 2 | 2 | ||||||||
MIRT450207 | ABHD15 | abhydrolase domain containing 15 | 2 | 2 | ||||||||
MIRT486622 | PDK3 | pyruvate dehydrogenase kinase 3 | 2 | 2 | ||||||||
MIRT489268 | TTLL1 | tubulin tyrosine ligase like 1 | 2 | 2 | ||||||||
MIRT493118 | MKNK2 | MAP kinase interacting serine/threonine kinase 2 | 2 | 4 | ||||||||
MIRT494571 | BAK1 | BCL2 antagonist/killer 1 | 2 | 2 | ||||||||
MIRT497396 | RALY | RALY heterogeneous nuclear ribonucleoprotein | 2 | 2 | ||||||||
MIRT504901 | CCDC86 | coiled-coil domain containing 86 | 2 | 2 | ||||||||
MIRT505193 | USP46 | ubiquitin specific peptidase 46 | 2 | 4 | ||||||||
MIRT506524 | MSANTD4 | Myb/SANT DNA binding domain containing 4 with coiled-coils | 2 | 6 | ||||||||
MIRT508163 | ABCC5 | ATP binding cassette subfamily C member 5 | 2 | 8 | ||||||||
MIRT508546 | PARVG | parvin gamma | 2 | 4 | ||||||||
MIRT509851 | BIRC5 | baculoviral IAP repeat containing 5 | 2 | 6 | ||||||||
MIRT510089 | PPWD1 | peptidylprolyl isomerase domain and WD repeat containing 1 | 2 | 8 | ||||||||
MIRT512095 | CRK | CRK proto-oncogene, adaptor protein | 2 | 4 | ||||||||
MIRT515382 | RPL7 | ribosomal protein L7 | 2 | 2 | ||||||||
MIRT519177 | SCO1 | SCO1, cytochrome c oxidase assembly protein | 2 | 2 | ||||||||
MIRT519965 | ZCCHC8 | zinc finger CCHC-type containing 8 | 2 | 2 | ||||||||
MIRT522293 | NKAP | NFKB activating protein | 2 | 2 | ||||||||
MIRT523161 | HMGB2 | high mobility group box 2 | 2 | 4 | ||||||||
MIRT525315 | FANCA | Fanconi anemia complementation group A | 2 | 2 | ||||||||
MIRT528195 | PLEKHM2 | pleckstrin homology and RUN domain containing M2 | 2 | 2 | ||||||||
MIRT532886 | ZNF451 | zinc finger protein 451 | 2 | 2 | ||||||||
MIRT538210 | CYR61 | cysteine rich angiogenic inducer 61 | 2 | 2 | ||||||||
MIRT539498 | ACTN4 | actinin alpha 4 | 2 | 2 | ||||||||
MIRT554602 | RRAGC | Ras related GTP binding C | 2 | 2 | ||||||||
MIRT562446 | DCTN6 | dynactin subunit 6 | 2 | 2 | ||||||||
MIRT562749 | AOC3 | amine oxidase, copper containing 3 | 2 | 2 | ||||||||
MIRT565800 | SEC14L5 | SEC14 like lipid binding 5 | 2 | 2 | ||||||||
MIRT565839 | SCML2 | Scm polycomb group protein like 2 | 2 | 2 | ||||||||
MIRT566073 | RCC2 | regulator of chromosome condensation 2 | 2 | 2 | ||||||||
MIRT566105 | RBPJ | recombination signal binding protein for immunoglobulin kappa J region | 2 | 2 | ||||||||
MIRT572408 | MRPS14 | mitochondrial ribosomal protein S14 | 2 | 2 | ||||||||
MIRT576199 | Vsig2 | V-set and immunoglobulin domain containing 2 | 2 | 2 | ||||||||
MIRT576314 | Acbd7 | acyl-Coenzyme A binding domain containing 7 | 2 | 2 | ||||||||
MIRT576652 | Mill2 | MHC I like leukocyte 2 | 1 | 1 | ||||||||
MIRT576860 | Socs6 | suppressor of cytokine signaling 6 | 2 | 2 | ||||||||
MIRT606820 | BICD2 | BICD cargo adaptor 2 | 2 | 2 | ||||||||
MIRT610739 | NUDT16 | nudix hydrolase 16 | 2 | 4 | ||||||||
MIRT614799 | RORA | RAR related orphan receptor A | 2 | 2 | ||||||||
MIRT619007 | NTMT1 | N-terminal Xaa-Pro-Lys N-methyltransferase 1 | 2 | 2 | ||||||||
MIRT619421 | NOS1AP | nitric oxide synthase 1 adaptor protein | 2 | 2 | ||||||||
MIRT620406 | MYO1H | myosin IH | 2 | 2 | ||||||||
MIRT624870 | ABHD13 | abhydrolase domain containing 13 | 2 | 2 | ||||||||
MIRT633528 | ZFP30 | ZFP30 zinc finger protein | 2 | 2 | ||||||||
MIRT633928 | DNAH9 | dynein axonemal heavy chain 9 | 2 | 2 | ||||||||
MIRT636612 | CLIC5 | chloride intracellular channel 5 | 2 | 2 | ||||||||
MIRT640617 | MIOX | myo-inositol oxygenase | 2 | 2 | ||||||||
MIRT642606 | C14orf180 | chromosome 14 open reading frame 180 | 2 | 2 | ||||||||
MIRT644625 | SRSF2 | serine and arginine rich splicing factor 2 | 2 | 2 | ||||||||
MIRT655114 | PHLDA3 | pleckstrin homology like domain family A member 3 | 2 | 2 | ||||||||
MIRT658977 | DNAJB5 | DnaJ heat shock protein family (Hsp40) member B5 | 2 | 4 | ||||||||
MIRT661496 | CHMP1B | charged multivesicular body protein 1B | 2 | 2 | ||||||||
MIRT662997 | TMEM59 | transmembrane protein 59 | 2 | 2 | ||||||||
MIRT663076 | SFR1 | SWI5 dependent homologous recombination repair protein 1 | 2 | 2 | ||||||||
MIRT665406 | WEE1 | WEE1 G2 checkpoint kinase | 2 | 2 | ||||||||
MIRT666107 | SSR1 | signal sequence receptor subunit 1 | 2 | 2 | ||||||||
MIRT669563 | ALDOA | aldolase, fructose-bisphosphate A | 2 | 2 | ||||||||
MIRT670071 | ZNF783 | zinc finger family member 783 | 2 | 2 | ||||||||
MIRT671193 | ZNF891 | zinc finger protein 891 | 2 | 2 | ||||||||
MIRT675362 | KLHL26 | kelch like family member 26 | 2 | 2 | ||||||||
MIRT678858 | LINC00598 | long intergenic non-protein coding RNA 598 | 2 | 2 | ||||||||
MIRT679444 | C19orf52 | translocase of inner mitochondrial membrane 29 | 2 | 2 | ||||||||
MIRT684164 | ALDH1B1 | aldehyde dehydrogenase 1 family member B1 | 2 | 2 | ||||||||
MIRT684544 | ZNF460 | zinc finger protein 460 | 2 | 2 | ||||||||
MIRT684675 | SLC2A11 | solute carrier family 2 member 11 | 2 | 2 | ||||||||
MIRT684972 | MINOS1 | mitochondrial inner membrane organizing system 1 | 2 | 2 | ||||||||
MIRT686007 | NEK4 | NIMA related kinase 4 | 2 | 2 | ||||||||
MIRT687759 | KIAA1328 | KIAA1328 | 2 | 2 | ||||||||
MIRT688962 | ATXN3 | ataxin 3 | 2 | 2 | ||||||||
MIRT689410 | UQCR11 | ubiquinol-cytochrome c reductase, complex III subunit XI | 2 | 2 | ||||||||
MIRT690002 | MMP17 | matrix metallopeptidase 17 | 2 | 2 | ||||||||
MIRT690166 | ELP3 | elongator acetyltransferase complex subunit 3 | 2 | 2 | ||||||||
MIRT690203 | C5orf45 | MRN complex interacting protein | 2 | 2 | ||||||||
MIRT690478 | ZNF33A | zinc finger protein 33A | 2 | 2 | ||||||||
MIRT690567 | MICA | MHC class I polypeptide-related sequence A | 2 | 2 | ||||||||
MIRT692161 | C10orf111 | chromosome 10 open reading frame 111 | 2 | 2 | ||||||||
MIRT693431 | PLGLB2 | plasminogen-like B2 | 2 | 2 | ||||||||
MIRT693548 | ZNF708 | zinc finger protein 708 | 2 | 2 | ||||||||
MIRT693698 | PLGLB1 | plasminogen-like B1 | 2 | 2 | ||||||||
MIRT695007 | HSPA6 | heat shock protein family A (Hsp70) member 6 | 2 | 2 | ||||||||
MIRT698526 | TFRC | transferrin receptor | 2 | 2 | ||||||||
MIRT699575 | SIKE1 | suppressor of IKBKE 1 | 2 | 2 | ||||||||
MIRT703447 | FYTTD1 | forty-two-three domain containing 1 | 2 | 2 | ||||||||
MIRT704398 | CTSS | cathepsin S | 2 | 2 | ||||||||
MIRT704486 | CPT1A | carnitine palmitoyltransferase 1A | 2 | 2 | ||||||||
MIRT709511 | IGF2 | insulin like growth factor 2 | 2 | 2 | ||||||||
MIRT709823 | STPG1 | sperm tail PG-rich repeat containing 1 | 2 | 2 | ||||||||
MIRT710860 | COQ7 | coenzyme Q7, hydroxylase | 2 | 2 | ||||||||
MIRT711894 | INSIG2 | insulin induced gene 2 | 2 | 2 | ||||||||
MIRT713797 | CPLX2 | complexin 2 | 2 | 2 | ||||||||
MIRT718801 | C1GALT1C1 | C1GALT1 specific chaperone 1 | 2 | 2 | ||||||||
MIRT719502 | SEC24B | SEC24 homolog B, COPII coat complex component | 2 | 2 | ||||||||
MIRT719723 | PDE6B | phosphodiesterase 6B | 2 | 2 | ||||||||
MIRT722205 | URM1 | ubiquitin related modifier 1 | 2 | 2 | ||||||||
MIRT723524 | CLPTM1L | CLPTM1 like | 2 | 2 | ||||||||
MIRT725473 | GRAP2 | GRB2-related adaptor protein 2 | 2 | 2 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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