>IER5|NM_016545|3'UTR
   1 GCCCTTGGCCCCCCTGCGGGGAGGAGGTGGAGCAGCGGGCGTCCCCGAAGTGAGGGCCAGGCCCTTCCCGGCTGCGAGGA
  81 CGCCCAGAGACCGCGGGCGCTGAGCGCGTTGGGCAGACTGGTTGCCTCTGGGCATCGCAAACTGCCCCCGGGCGCATCTG
 161 GCTTATCTGGAGACCTGGGAGCTTGAGGCTCATTGGAAGAGGACGATCGTTAACTTTTGTGTTTGTGTATGTCTGTGTAT
 241 GTAAGTTTGTCTTGTGGCATTAAGACTATTTTGCTCTTCTGGAATGCACCACTCCTTCCCCAGGGTTTAAGAGATTGGGG
 321 GCAGACAGGGACTTTCCTCTGCCGGCTGCGTGGAGAAGGAAGCGGCTAAAAGGTTTGGGCCGGGGAGTTCCCCATTCGTT
 401 CTCCGGGGAGGGAGGGACTTTACACCTACCCCTCACCGGAAAGCTAGACCCGCTTCAGGGCCAGGAGTGGCGTTTCCGCA
 481 CAGGATTTCCTAAGACGAGAGGGATTTAGCCAAGAGCACAGACTTGGATTCCTTCTGTCCCTCCCCACCTTTCTCTCCCA
 561 ATGAAGAAACGTTTATATCCTGTGATTACTCTAGGAAAGGGGCGTATTAACGGTCCTTTGCTTCCCCGCAGGGGAGAAAA
 641 AGCTTGACGAACTTCACAGAAGAGTTCAAGCTTGGAAATAATGGAGTTTATAGAGAAAAGATATATTTTTAAAAGCTCTA
 721 GGTCAGAAGTACTACACAGTGCTTTTAAAAAGTGTTTAATGAGAGTTTACAGACAGAAGCCCGAAGTGGAAAGACCTTAT
 801 GGTTTTGTAGATATGGTGACTCCAGTCTTTGTTGTATAAAGGTTGGGGGAGCTGATAAGGTTTTTGTACAGTATTTTCTC
 881 CTTCGTTGTATTGATTTTTGTATAAAAATGTAACTTCTACGTGTCTAACACGTATTAAATATTTTGAAGCAACGTAAAAA
 961 AAAAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' gacgUUC--CCUCUCUUCUUUCUGu 5' ||| || || :||||||| Target 5' acagAAGCCCGA-AGTGGAAAGACc 3'	773 - 796	152.00	-8.50
2	miRNA 3' gacguucCCUCUCUUCUUUCUGu 5' ||:||| :||::||| Target 5' gttctccGGGGAG-GGAGGGACt 3'	398 - 419	127.00	-19.10
3	miRNA 3' gaCGUUC-CCUCUCUUCUUUCUgu 5' || :| | | |:||||:|| Target 5' ctGCCGGCTGCGTGGAGAAGGAag 3'	339 - 362	121.00	-19.90

Mutant ID	Mutant Position	Mutant Source
COSN30448001	14	COSMIC
COSN26979605	15	COSMIC
COSN30177791	15	COSMIC
COSN30176299	117	COSMIC
COSN6010779	142	COSMIC
COSN31609266	248	COSMIC
COSN8409255	285	COSMIC
COSN31555778	322	COSMIC
COSN31488184	398	COSMIC
COSN30539851	409	COSMIC
COSN31569290	450	COSMIC
COSN31480350	498	COSMIC
COSN31562971	588	COSMIC
COSN30135595	593	COSMIC
COSN30130696	701	COSMIC
COSN31490579	723	COSMIC
COSN5734356	745	COSMIC
COSN30173810	817	COSMIC
COSN30141426	877	COSMIC
COSN15103019	911	COSMIC
COSN27677065	1035	COSMIC
COSN6010780	1579	COSMIC
COSN17807444	1823	COSMIC
COSN2480442	2335	COSMIC
COSN7187333	2569	COSMIC
COSN7187334	2570	COSMIC
COSN7466889	2587	COSMIC
rs61809230	2895	GWAS

Mutant ID	Mutant Position	Mutant Source
rs759197765	5	dbSNP
rs761886733	9	dbSNP
rs370832067	10	dbSNP
rs962595441	11	dbSNP
rs752981134	12	dbSNP
rs763127563	14	dbSNP
rs555051422	19	dbSNP
rs373090777	20	dbSNP
rs1349317605	21	dbSNP
rs765732512	23	dbSNP
rs974102533	23	dbSNP
rs750811499	24	dbSNP
rs1300739606	27	dbSNP
rs1255307929	33	dbSNP
rs1331580022	34	dbSNP
rs1235416493	37	dbSNP
rs756602231	39	dbSNP
rs1341130243	41	dbSNP
rs1205619952	45	dbSNP
rs780536811	46	dbSNP
rs747854048	47	dbSNP
rs770931269	49	dbSNP
rs1208692585	52	dbSNP
rs771802265	53	dbSNP
rs1051616574	57	dbSNP
rs1241048431	61	dbSNP
rs912755726	62	dbSNP
rs942861075	63	dbSNP
rs941527842	64	dbSNP
rs1039821443	65	dbSNP
rs141671790	69	dbSNP
rs1430791491	70	dbSNP
rs899570951	71	dbSNP
rs996578908	74	dbSNP
rs558194455	77	dbSNP
rs1029264187	78	dbSNP
rs1469869143	82	dbSNP
rs890808933	85	dbSNP
rs1486190469	86	dbSNP
rs1364224727	87	dbSNP
rs1279999615	92	dbSNP
rs1209392076	93	dbSNP
rs1427148089	109	dbSNP
rs1324232824	113	dbSNP
rs777629689	115	dbSNP
rs1370815827	117	dbSNP
rs1294713945	119	dbSNP
rs1415221609	122	dbSNP
rs576568510	125	dbSNP
rs1428810104	129	dbSNP
rs150437495	136	dbSNP
rs999306338	137	dbSNP
rs957351689	145	dbSNP
rs372188769	147	dbSNP
rs562262533	150	dbSNP
rs574424498	154	dbSNP
rs1244945461	155	dbSNP
rs1022826818	157	dbSNP
rs1475635256	163	dbSNP
rs74795403	165	dbSNP
rs373948726	167	dbSNP
rs1289371597	168	dbSNP
rs1361453736	169	dbSNP
rs1186182585	173	dbSNP
rs1223151290	177	dbSNP
rs1282862408	178	dbSNP
rs1230706550	187	dbSNP
rs1243197336	190	dbSNP
rs1214505469	191	dbSNP
rs1444776020	192	dbSNP
rs1274457023	197	dbSNP
rs770593116	205	dbSNP
rs1012103046	206	dbSNP
rs149573653	209	dbSNP
rs1383699737	215	dbSNP
rs962624687	216	dbSNP
rs974387566	217	dbSNP
rs1362620059	219	dbSNP
rs1288190564	226	dbSNP
rs1428489173	230	dbSNP
rs1242320514	231	dbSNP
rs1327930746	234	dbSNP
rs752460885	244	dbSNP
rs1462174771	253	dbSNP
rs1417397396	256	dbSNP
rs1163311371	260	dbSNP
rs1443677452	269	dbSNP
rs376514854	274	dbSNP
rs762060319	277	dbSNP
rs1186469258	280	dbSNP
rs960703203	298	dbSNP
rs983015078	299	dbSNP
rs1270845985	306	dbSNP
rs1229396818	311	dbSNP
rs951442540	315	dbSNP
rs986935277	316	dbSNP
rs1212741714	317	dbSNP
rs1373017725	321	dbSNP
rs1305792714	324	dbSNP
rs552001806	328	dbSNP
rs1394078042	331	dbSNP
rs1038928142	333	dbSNP
rs1463677057	337	dbSNP
rs1355515981	341	dbSNP
rs564168441	346	dbSNP
rs1399007494	348	dbSNP
rs767731594	349	dbSNP
rs1247389342	351	dbSNP
rs1453241764	360	dbSNP
rs1320424484	364	dbSNP
rs921465868	367	dbSNP
rs1452229575	373	dbSNP
rs942766190	380	dbSNP
rs750445977	382	dbSNP
rs531409951	383	dbSNP
rs1350043639	386	dbSNP
rs914708656	391	dbSNP
rs549996641	395	dbSNP
rs1235184259	402	dbSNP
rs1488256577	404	dbSNP
rs947697866	406	dbSNP
rs1393368930	407	dbSNP
rs1374258875	408	dbSNP
rs1298026515	409	dbSNP
rs1428219446	419	dbSNP
rs546855511	422	dbSNP
rs1189699989	425	dbSNP
rs1174837561	426	dbSNP
rs1375432564	427	dbSNP
rs1183274589	430	dbSNP
rs567906971	433	dbSNP
rs1426877295	437	dbSNP
rs1249373761	438	dbSNP
rs1175308315	440	dbSNP
rs1486517898	445	dbSNP
rs1280717736	448	dbSNP
rs1312582827	450	dbSNP
rs1052823585	451	dbSNP
rs893032334	453	dbSNP
rs1317822250	457	dbSNP
rs535397154	461	dbSNP
rs1278870283	462	dbSNP
rs1408176438	464	dbSNP
rs1357788771	466	dbSNP
rs1313794458	473	dbSNP
rs1414725371	479	dbSNP
rs1400674731	480	dbSNP
rs902205706	482	dbSNP
rs1159859322	485	dbSNP
rs751070407	487	dbSNP
rs1011518342	492	dbSNP
rs756174417	495	dbSNP
rs1156537206	497	dbSNP
rs1348515895	500	dbSNP
rs1186891428	501	dbSNP
rs1440690466	502	dbSNP
rs547257652	503	dbSNP
rs1207017399	509	dbSNP
rs896238307	510	dbSNP
rs1284385973	513	dbSNP
rs1011968912	517	dbSNP
rs1035103524	527	dbSNP
rs1201405346	528	dbSNP
rs567188266	536	dbSNP
rs1462930717	537	dbSNP
rs1233051378	538	dbSNP
rs539777579	538	dbSNP
rs1339266562	540	dbSNP
rs1023504642	542	dbSNP
rs748073829	543	dbSNP
rs1320825323	544	dbSNP
rs908569692	546	dbSNP
rs1317877232	569	dbSNP
rs1462707492	574	dbSNP
rs1188747671	581	dbSNP
rs1418110558	586	dbSNP
rs898326569	591	dbSNP
rs1471672077	596	dbSNP
rs1374531213	600	dbSNP
rs780177301	604	dbSNP
rs921402895	605	dbSNP
rs1160331596	608	dbSNP
rs1412820888	611	dbSNP
rs1274007732	612	dbSNP
rs1469424692	617	dbSNP
rs1354714702	618	dbSNP
rs995462926	620	dbSNP
rs1051243788	623	dbSNP
rs116181702	624	dbSNP
rs1294846715	629	dbSNP
rs951302785	630	dbSNP
rs370719345	632	dbSNP
rs143392702	635	dbSNP
rs1324781890	636	dbSNP
rs1403200415	646	dbSNP
rs1171297884	659	dbSNP
rs1398368444	660	dbSNP
rs537139574	663	dbSNP
rs556030648	666	dbSNP
rs574262387	669	dbSNP
rs1475075944	681	dbSNP
rs1388245092	683	dbSNP
rs1186797876	691	dbSNP
rs541768256	692	dbSNP
rs1221153977	700	dbSNP
rs1450018112	702	dbSNP
rs1275568306	704	dbSNP
rs560054760	705	dbSNP
rs1288369458	709	dbSNP
rs975461636	712	dbSNP
rs1235923932	719	dbSNP
rs754337695	725	dbSNP
rs1301976882	731	dbSNP
rs1221221886	745	dbSNP
rs1264598688	746	dbSNP
rs905653629	755	dbSNP
rs1337145015	757	dbSNP
rs947529329	757	dbSNP
rs1483399017	765	dbSNP
rs977587704	768	dbSNP
rs924875301	771	dbSNP
rs1368682142	773	dbSNP
rs755485951	775	dbSNP
rs1175463748	776	dbSNP
rs571956595	780	dbSNP
rs1035554491	783	dbSNP
rs1395774596	788	dbSNP
rs1477196702	792	dbSNP
rs545738681	792	dbSNP
rs1234815944	793	dbSNP
rs1045078	795	dbSNP
rs1250294162	796	dbSNP
rs368929841	801	dbSNP
rs1482922713	809	dbSNP
rs78185242	814	dbSNP
rs962754155	815	dbSNP
rs947822804	818	dbSNP
rs1028531008	821	dbSNP
rs954329520	826	dbSNP
rs1253253631	828	dbSNP
rs183721575	835	dbSNP
rs1352446882	839	dbSNP
rs986917067	848	dbSNP
rs898362611	850	dbSNP
rs531477169	858	dbSNP
rs549452649	859	dbSNP
rs1431678255	862	dbSNP
rs1314414498	867	dbSNP
rs41268464	872	dbSNP
rs1412807350	874	dbSNP
rs1410503388	881	dbSNP
rs1159391737	889	dbSNP
rs1472347142	890	dbSNP
rs568417293	901	dbSNP
rs1167060351	903	dbSNP
rs41268466	911	dbSNP
rs1486321382	913	dbSNP
rs1237379885	917	dbSNP
rs914189667	918	dbSNP
rs1284299594	920	dbSNP
rs964011526	922	dbSNP
rs1308554169	954	dbSNP
rs947074555	958	dbSNP
rs547297136	966	dbSNP
rs1021677867	983	dbSNP
rs1300989755	984	dbSNP
rs1432754455	985	dbSNP
rs1347073308	990	dbSNP
rs1318991361	994	dbSNP
rs565588771	998	dbSNP
rs968807301	1006	dbSNP
rs1456964208	1011	dbSNP
rs1410024152	1021	dbSNP
rs867304132	1021	dbSNP
rs539347620	1022	dbSNP
rs1167599022	1024	dbSNP
rs1044121516	1036	dbSNP
rs1413462741	1042	dbSNP
rs977994091	1053	dbSNP
rs1344866679	1056	dbSNP
rs551791206	1059	dbSNP
rs1402524991	1070	dbSNP
rs905768294	1071	dbSNP
rs188140991	1073	dbSNP
rs1463320924	1080	dbSNP
rs537579513	1081	dbSNP
rs555869362	1082	dbSNP
rs574300927	1096	dbSNP
rs1312371301	1097	dbSNP
rs1320920071	1098	dbSNP
rs1310268139	1099	dbSNP
rs1056967739	1106	dbSNP
rs1362552053	1108	dbSNP
rs886290967	1110	dbSNP
rs139018582	1111	dbSNP
rs1386787872	1114	dbSNP
rs1328199182	1116	dbSNP
rs1015685369	1128	dbSNP
rs1251051107	1134	dbSNP
rs917611055	1143	dbSNP
rs1207811802	1144	dbSNP
rs1420846972	1150	dbSNP
rs1187433429	1154	dbSNP
rs1447112643	1156	dbSNP
rs553707243	1161	dbSNP
rs1254283456	1164	dbSNP
rs181325398	1165	dbSNP
rs1044726673	1168	dbSNP
rs1263513356	1169	dbSNP
rs1206348475	1172	dbSNP
rs1308045957	1179	dbSNP
rs545369016	1183	dbSNP
rs926928369	1188	dbSNP
rs1363153208	1198	dbSNP
rs1197317685	1201	dbSNP
rs1387579998	1208	dbSNP
rs66779133	1210	dbSNP
rs1433931322	1212	dbSNP
rs1192757145	1213	dbSNP
rs1463824950	1216	dbSNP
rs931040913	1220	dbSNP
rs954278920	1228	dbSNP
rs1463316096	1230	dbSNP
rs1396371469	1233	dbSNP
rs987033658	1236	dbSNP
rs1264785805	1239	dbSNP
rs575927868	1240	dbSNP
rs1480848439	1242	dbSNP
rs1250744903	1248	dbSNP
rs1202941651	1259	dbSNP
rs369654050	1269	dbSNP
rs886940214	1272	dbSNP
rs1008139448	1273	dbSNP
rs1422701200	1281	dbSNP
rs1304377965	1285	dbSNP
rs142101753	1287	dbSNP
rs186328090	1290	dbSNP
rs1234050802	1303	dbSNP
rs1311872015	1304	dbSNP
rs529054721	1310	dbSNP
rs1299440085	1316	dbSNP
rs1040922965	1323	dbSNP
rs899731538	1327	dbSNP
rs1292982978	1331	dbSNP
rs191582300	1339	dbSNP
rs1307744062	1348	dbSNP
rs1327881629	1356	dbSNP
rs1408555659	1360	dbSNP
rs1231034318	1369	dbSNP
rs1298841548	1370	dbSNP
rs1160878123	1371	dbSNP
rs1302958484	1371	dbSNP
rs368454952	1371	dbSNP
rs60601152	1371	dbSNP
rs759865193	1371	dbSNP
rs914323184	1371	dbSNP
rs373156866	1384	dbSNP
rs1410973108	1386	dbSNP
rs1021541217	1387	dbSNP
rs1486650526	1389	dbSNP
rs1258102039	1392	dbSNP
rs777557107	1397	dbSNP
rs1201158214	1401	dbSNP
rs1482981441	1402	dbSNP
rs1254290058	1408	dbSNP
rs1241461722	1409	dbSNP
rs1267233729	1410	dbSNP
rs1330027322	1412	dbSNP
rs1288186575	1422	dbSNP
rs1226905432	1433	dbSNP
rs1488847765	1433	dbSNP
rs1357965620	1437	dbSNP
rs926969513	1441	dbSNP
rs1395916307	1442	dbSNP
rs1399440233	1448	dbSNP
rs559272901	1453	dbSNP
rs114779008	1454	dbSNP
rs1451601757	1457	dbSNP
rs1188918808	1462	dbSNP
rs74134323	1465	dbSNP
rs940533285	1466	dbSNP
rs1186793800	1467	dbSNP
rs757136384	1467	dbSNP
rs1421918936	1469	dbSNP
rs1254188667	1477	dbSNP
rs1210663076	1481	dbSNP
rs1465543193	1482	dbSNP
rs1267714194	1485	dbSNP
rs960214893	1492	dbSNP
rs1442636589	1494	dbSNP
rs1207002108	1497	dbSNP
rs993110586	1506	dbSNP
rs1337440947	1508	dbSNP
rs181674118	1512	dbSNP
rs1037973396	1516	dbSNP
rs970276300	1518	dbSNP
rs980437707	1519	dbSNP
rs919568441	1520	dbSNP
rs931090897	1523	dbSNP
rs1297183052	1528	dbSNP
rs530892324	1540	dbSNP
rs1435153304	1546	dbSNP
rs569235938	1566	dbSNP
rs1452404487	1568	dbSNP
rs1462659155	1571	dbSNP
rs549508893	1578	dbSNP
rs1391198121	1582	dbSNP
rs866484340	1587	dbSNP
rs1162376864	1590	dbSNP
rs4287133	1597	dbSNP
rs1386631419	1602	dbSNP
rs1374689699	1607	dbSNP
rs1190649806	1609	dbSNP
rs995573820	1614	dbSNP
rs797020925	1616	dbSNP
rs1435396659	1617	dbSNP
rs138234068	1621	dbSNP
rs1453482928	1627	dbSNP
rs1382508271	1636	dbSNP
rs773419943	1639	dbSNP
rs1209612248	1648	dbSNP
rs889800204	1650	dbSNP
rs535120686	1652	dbSNP
rs1229730273	1659	dbSNP
rs187235793	1661	dbSNP
rs1363871336	1666	dbSNP
rs1294684908	1668	dbSNP
rs376839785	1670	dbSNP
rs146369502	1671	dbSNP
rs899761268	1673	dbSNP
rs1322731940	1674	dbSNP
rs996684655	1679	dbSNP
rs539349304	1682	dbSNP
rs1398297684	1685	dbSNP
rs558256317	1692	dbSNP
rs1428953862	1693	dbSNP
rs1389644451	1696	dbSNP
rs1479166381	1697	dbSNP
rs1247476811	1712	dbSNP
rs1019828523	1724	dbSNP
rs1450263167	1724	dbSNP
rs760929502	1727	dbSNP
rs766422701	1729	dbSNP
rs956527208	1730	dbSNP
rs753885269	1731	dbSNP
rs1206508147	1740	dbSNP
rs999366561	1744	dbSNP
rs1259068769	1745	dbSNP
rs139850261	1747	dbSNP
rs1022236818	1748	dbSNP
rs968477132	1750	dbSNP
rs1273899010	1753	dbSNP
rs960083977	1758	dbSNP
rs191155351	1762	dbSNP
rs6658698	1770	dbSNP
rs573441443	1771	dbSNP
rs1183431406	1775	dbSNP
rs1414403816	1777	dbSNP
rs141191116	1783	dbSNP
rs1156254703	1790	dbSNP
rs907778152	1794	dbSNP
rs1172245803	1795	dbSNP
rs1457385073	1798	dbSNP
rs1477126448	1805	dbSNP
rs940633712	1810	dbSNP
rs1335674351	1819	dbSNP
rs1037523371	1820	dbSNP
rs973645229	1821	dbSNP
rs559526591	1822	dbSNP
rs931370396	1846	dbSNP
rs1212791267	1847	dbSNP
rs1442523330	1848	dbSNP
rs1277209058	1849	dbSNP
rs1027107083	1851	dbSNP
rs889746456	1856	dbSNP
rs1227521403	1868	dbSNP
rs1288821811	1868	dbSNP
rs781199891	1870	dbSNP
rs1041475223	1871	dbSNP
rs952395282	1872	dbSNP
rs1380473914	1873	dbSNP
rs982968413	1875	dbSNP
rs1391343251	1878	dbSNP
rs150753992	1880	dbSNP
rs534460909	1881	dbSNP
rs943751815	1882	dbSNP
rs969305378	1889	dbSNP
rs545303553	1893	dbSNP
rs1034102514	1901	dbSNP
rs959812482	1905	dbSNP
rs1473385708	1910	dbSNP
rs1236439909	1913	dbSNP
rs1207377490	1914	dbSNP
rs1321723723	1916	dbSNP
rs183366823	1921	dbSNP
rs1211927205	1927	dbSNP
rs921086904	1929	dbSNP
rs932607715	1934	dbSNP
rs1480237367	1937	dbSNP
rs1175418767	1938	dbSNP
rs765667002	1939	dbSNP
rs904548886	1948	dbSNP
rs907557399	1951	dbSNP
rs1283712119	1952	dbSNP
rs961964519	1958	dbSNP
rs1253978322	1960	dbSNP
rs1472627786	1974	dbSNP
rs934694945	1986	dbSNP
rs530931966	1988	dbSNP
rs1053508282	1989	dbSNP
rs549447441	1992	dbSNP
rs1413699892	1994	dbSNP
rs920409155	1996	dbSNP
rs895908003	1997	dbSNP
rs1248030934	2000	dbSNP
rs567656234	2006	dbSNP
rs1178620173	2022	dbSNP
rs1463229983	2024	dbSNP
rs911298098	2025	dbSNP
rs369639034	2031	dbSNP
rs1287620380	2033	dbSNP
rs906028263	2037	dbSNP
rs1041030487	2049	dbSNP
rs891807333	2066	dbSNP
rs946290956	2067	dbSNP
rs1352926046	2069	dbSNP
rs995522323	2070	dbSNP
rs115920657	2075	dbSNP
rs1282763135	2076	dbSNP
rs904650902	2083	dbSNP
rs1221170465	2084	dbSNP
rs1349603820	2086	dbSNP
rs1162228290	2093	dbSNP
rs1432125830	2107	dbSNP
rs1001700582	2119	dbSNP
rs1278991979	2132	dbSNP
rs1167388648	2143	dbSNP
rs571835959	2149	dbSNP
rs1034546636	2150	dbSNP
rs951008633	2153	dbSNP
rs1014439749	2158	dbSNP
rs1202259809	2160	dbSNP
rs1014564060	2164	dbSNP
rs1483362516	2172	dbSNP
rs1483331098	2173	dbSNP
rs962024660	2174	dbSNP
rs1266176575	2177	dbSNP
rs1306043639	2178	dbSNP
rs973307465	2180	dbSNP
rs1219966669	2185	dbSNP
rs1367383496	2187	dbSNP
rs1027984059	2190	dbSNP
rs546249896	2193	dbSNP
rs982470716	2194	dbSNP
rs1371677656	2196	dbSNP
rs1306943123	2199	dbSNP
rs35457825	2206	dbSNP
rs1015641510	2213	dbSNP
rs985912429	2227	dbSNP
rs1460010353	2230	dbSNP
rs758818813	2231	dbSNP
rs1409735540	2232	dbSNP
rs1432190139	2237	dbSNP
rs1422862814	2238	dbSNP
rs1184046064	2241	dbSNP
rs555639546	2243	dbSNP
rs1250971941	2256	dbSNP
rs1202867296	2257	dbSNP
rs977254627	2258	dbSNP
rs538825920	2260	dbSNP
rs1311802673	2266	dbSNP
rs921117881	2275	dbSNP
rs769923932	2288	dbSNP
rs978971779	2293	dbSNP
rs1288282217	2303	dbSNP
rs925913286	2313	dbSNP
rs934534883	2325	dbSNP
rs1390098268	2326	dbSNP
rs1403753091	2332	dbSNP
rs772137526	2341	dbSNP
rs557633971	2344	dbSNP
rs1465272511	2347	dbSNP
rs1403437235	2350	dbSNP
rs1348213189	2381	dbSNP
rs913197407	2383	dbSNP
rs946040409	2384	dbSNP
rs1451127003	2393	dbSNP
rs1053135103	2397	dbSNP
rs895814956	2410	dbSNP
rs139026337	2415	dbSNP
rs1425436008	2416	dbSNP
rs1258411003	2422	dbSNP
rs1182489162	2428	dbSNP
rs1483084609	2440	dbSNP
rs746626337	2442	dbSNP
rs904763814	2447	dbSNP
rs536537528	2450	dbSNP
rs1267847077	2452	dbSNP
rs1299580313	2452	dbSNP
rs1342721274	2454	dbSNP
rs906059547	2464	dbSNP
rs113439468	2465	dbSNP
rs1312899968	2466	dbSNP
rs1394935187	2473	dbSNP
rs771460666	2477	dbSNP
rs745378058	2483	dbSNP
rs886699545	2487	dbSNP
rs1387662530	2493	dbSNP
rs1452828835	2493	dbSNP
rs1457407493	2493	dbSNP
rs1005537901	2504	dbSNP
rs1015093302	2505	dbSNP
rs1241133869	2511	dbSNP
rs1015251533	2518	dbSNP
rs897614035	2519	dbSNP
rs1190783073	2525	dbSNP
rs1465498145	2527	dbSNP
rs1247845056	2530	dbSNP
rs1203753148	2533	dbSNP
rs964993538	2537	dbSNP
rs994904579	2539	dbSNP
rs1187014809	2545	dbSNP
rs997918305	2546	dbSNP
rs1392434905	2547	dbSNP
rs573278636	2556	dbSNP
rs1450567941	2557	dbSNP
rs1435422794	2560	dbSNP
rs1028040429	2565	dbSNP
rs1363971445	2565	dbSNP
rs1027303842	2566	dbSNP
rs746133138	2566	dbSNP
rs751900194	2566	dbSNP
rs953907444	2566	dbSNP
rs769715085	2569	dbSNP
rs775276712	2570	dbSNP
rs956019038	2577	dbSNP
rs989131943	2583	dbSNP
rs1324333639	2587	dbSNP
rs1191232667	2589	dbSNP
rs552884726	2591	dbSNP
rs1018703158	2596	dbSNP
rs1268446609	2609	dbSNP
rs1433192330	2615	dbSNP
rs917279103	2622	dbSNP
rs186957764	2629	dbSNP
rs1044389517	2630	dbSNP
rs1365664355	2634	dbSNP
rs1245059991	2635	dbSNP
rs927364404	2642	dbSNP
rs930687614	2644	dbSNP
rs1341090386	2648	dbSNP
rs1268055415	2649	dbSNP
rs1234876051	2652	dbSNP
rs913303331	2654	dbSNP
rs1049263418	2658	dbSNP
rs1358444513	2658	dbSNP
rs946186303	2663	dbSNP
rs1372329029	2665	dbSNP
rs979153344	2672	dbSNP
rs1430504212	2676	dbSNP
rs1395782004	2677	dbSNP
rs886603707	2680	dbSNP
rs925963586	2696	dbSNP
rs768410727	2699	dbSNP
rs1378626503	2702	dbSNP
rs189689433	2708	dbSNP
rs1479780868	2714	dbSNP
rs1254574352	2717	dbSNP
rs1206423747	2725	dbSNP
rs1040588582	2726	dbSNP
rs902073893	2731	dbSNP
rs1444864825	2738	dbSNP
rs35443327	2742	dbSNP
rs1345250647	2750	dbSNP
rs1282479063	2752	dbSNP
rs1221454253	2761	dbSNP
rs997823497	2768	dbSNP
rs917385507	2777	dbSNP
rs950263272	2777	dbSNP
rs1409095811	2778	dbSNP
rs1354033849	2788	dbSNP
rs1248332661	2804	dbSNP
rs774179639	2806	dbSNP
rs1416419075	2808	dbSNP
rs760835811	2809	dbSNP
rs1465622557	2814	dbSNP
rs1027907973	2831	dbSNP
rs1367208010	2832	dbSNP
rs1474074959	2837	dbSNP
rs1403175804	2838	dbSNP
rs766540284	2840	dbSNP
rs1468578763	2845	dbSNP
rs1414559334	2848	dbSNP
rs1036568736	2851	dbSNP
rs1470026347	2853	dbSNP
rs760174581	2863	dbSNP
rs1230724046	2868	dbSNP
rs1189019260	2874	dbSNP
rs556663917	2876	dbSNP
rs764520802	2879	dbSNP
rs1407363996	2882	dbSNP
rs577777471	2894	dbSNP
rs61809230	2895	dbSNP
rs1268476806	2896	dbSNP
rs1223197353	2898	dbSNP
rs1389734734	2902	dbSNP
rs1032885963	2905	dbSNP
rs1277923025	2908	dbSNP
rs1451616532	2908	dbSNP
rs1048684307	2911	dbSNP
rs888792752	2915	dbSNP
rs1007396576	2916	dbSNP
rs563496435	2930	dbSNP
rs183015808	2932	dbSNP
rs1160332467	2936	dbSNP
rs1448688735	2937	dbSNP
rs1406411798	2940	dbSNP
rs1165451117	2941	dbSNP
rs1472845817	2943	dbSNP
rs751187204	2950	dbSNP
rs988756033	2950	dbSNP
rs1241945107	2952	dbSNP
rs966025893	2954	dbSNP
rs1465961145	2957	dbSNP
rs542929313	2962	dbSNP
rs1248457519	2970	dbSNP
rs1278375677	2978	dbSNP
rs1009806601	2980	dbSNP
rs1315115317	2988	dbSNP
rs765412134	2993	dbSNP
rs1228746760	3001	dbSNP
rs72637262	3003	dbSNP
rs1400252977	3008	dbSNP
rs147628599	3014	dbSNP

- Genes & development, 2013

When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.