| pre-miRNA Information | |
|---|---|
| pre-miRNA | hsa-mir-921 |
| Genomic Coordinates | chr1: 166154743 - 166154798 |
| Synonyms | MIRN921, hsa-mir-921, MIR921 |
| Description | Homo sapiens miR-921 stem-loop |
| Comment | None |
| RNA Secondary Structure |  |
| Mature miRNA Information | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mature miRNA | hsa-miR-921 | ||||||||||||
| Sequence | 2| CUAGUGAGGGACAGAACCAGGAUUC |26 | ||||||||||||
| Evidence | Experimental | ||||||||||||
| Experiments | Cloned | DRVs in miRNA |
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| SNPs in miRNA |
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| Putative Targets | |||||||||||||
| miRNA Expression profile | |
|---|---|
| Human miRNA Tissue Atlas | |
| Circulating MicroRNA Expression Profiling | |
| Gene Information | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Symbol | TIPRL | ||||||||||||||||||||
| Synonyms | TIP, TIP41, TIPRL1 | ||||||||||||||||||||
| Description | TOR signaling pathway regulator | ||||||||||||||||||||
| Transcript | NM_152902 | ||||||||||||||||||||
| Other Transcripts | NM_001031800 | ||||||||||||||||||||
| Expression | |||||||||||||||||||||
| Putative miRNA Targets on TIPRL | |||||||||||||||||||||
| 3'UTR of TIPRL (miRNA target sites are highlighted) |
>TIPRL|NM_152902|3'UTR 1 AATGTGATACAACATATACTCACTATGGAATCTGACTGGACACCTTGGCTATTTGTAAGGGGTTATTTTTATTATGAGAA 81 TTAATTGCCTTGTTTATGTACAGATTTTCTGTAGCCTTAAAGGAAAAAAAAATAAAGATCGTTACAGGCAGGTTTCACTC 161 AACTGCTGTTTGTACTGTCTGTCTTCACATTCATATTCCAGATTTATATTTTCTGGAGTTAAATTTGGATGATTTCTAAA 241 TTATCACAAAGTGGGACCTCAGCAGTAGTGATGTGTGTGTCTCATGAGCAGTGAGCACAGTCTGCATTCATCATGAAACA 321 CTATCTTCTACCAGGAGGAGGTTAATGTAAATCACCAAATCCCAATGCCTTGTGACTTTCATAGGATTCCTGATCATGCA 401 TGTTGATGTACTGGCTCTTCACTTTGGGCTTTCTGATGTTTATTCACACCTTTGGAGAGTTGCAACTTGCCACATACGAA 481 ATTAGTCTCATAGTGTAGTGAACTTCAACCCCAAAATTTTAAAAATGTATTTCCCCCCAGTTTTAAATTGCCTTTGAAAT 561 TTAAAAAAAAAAAATTTAGACTTAGTACCAGAACCAAAAATACCTAGATTTTTGGAGAACTTATTACATACATAGAAACA 641 TGAATATGGTTTACCACTGTGTGTGTGTAGGATGTTAGAATTATCTGTCCTCCATCTTTAGGTGCTTTTTCTTAAATGTG 721 GTTCTACTTATACTGATATTTTTAATTTCCATCTTCCATGCAACCTCAGAGTGAATAAACCCCTTAAATTTGGTGCTGGT 801 TCGAAAAAGTCTAAAGGGTTTATTAGGGGTTGTTTTTCGCAAATATTACATCAATCCTTAAAGCAACAAGATTAATTTTC 881 TGCTTAAAATATTTGGGAAGATAGGTAAGGAGGAGGGGGTTTTAAAATATAAAAGCAAGTTTTTCTATTTTAAGGTGCAT 961 ATTTGTAACATTACAGGGGATGAAGTAAAATGTAATTAATTAGCACAAGACTTAGGGAGTACAGAAAATACACAGAAGTA 1041 AATTTCAAATCCATTTGATTGTTTTCATAGTGACCCCTTAGTCATTTTATATTCTTGTCTGCCTTTCTAGAAAAATGGTT 1121 GTCATAAAGTGGAAAGTGATTAAAAAGCATCAAATAAGAAATTATTTCAAGTGGATTTTTAAAAATATTTTTTTGAAGGG 1201 CAGGGGGAAAATTCACCCCTTTTCTGATTTGAAATATGTTGTACATATTTCCATTTGATGGATAAATCATTAAGTAAGAA 1281 TATTTGATTTAAAGTATTAGCCAACCTCTTCAGGTATTAGCCTGAAGATAAATTTTAACAAAACATATACACTTGGGTAT 1361 CCGTCATTGCTCAAACTCTATAGTGTATTGCTGGAGCCAATAGGCAGGGTATATTTTATTAGCTAAATTTGATATTTGTC 1441 TTCTGCCTTCTGTATCACCTCCAAGCTATAGGAAATCAGGATTTTGTTGGCTTTAAGAAAACACATGGTATGTTCACTGT 1521 ATATTAAATATACCTGTATTTAATGTTTTCTCTTAGGACAGAAAAGTAGACACACACACACACACACACACACATGTTGT 1601 GTTCAGCTTTCTGTTTTATATTATTTGCCATTGAGATTAGAATAGAACAGGCTCTATTCATGCAAACTATATGAAATGAA 1681 AAACTTTTAAGACTCTTCATTAATTGGAGCTTCTGGGCAACATCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT 1761 ATACAGACATTTTTTTTTTAACTTGTTGATTCAGATGTCTTGGTCCCTGAATAGTCCTAGATTACTTATTTTGAGAATTC 1841 ATTGTTAAAAATTACAGGGAATTAAAATAATTGCCTTTTTTTTTTTTAGAGGGTAAGAGATGGGTAGAAGAGTATGCCTC 1921 TGAAAATTTTATTAGTTTATTCTTGTGGAGAATACCAAGAAAATGTGTATTTGCCCATTGCTAAATATGATATATGCCAT 2001 TTTGTATTTATTTGTCCCAAGTGTCTTTTTGTAAGAGGAGAATAAACAATAAGGAATTACTGATCAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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| miRNA-target interactions (Predicted by miRanda) |
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| DRVs in gene 3'UTRs | |||||||||||||||||||||
| SNPs in gene 3'UTRs | |||||||||||||||||||||
| Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
|---|---|---|---|---|---|---|---|
| miRNA:Target | ---- | ||||||
| Validation Method |
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| Conditions | HEK293 | ||||||
| Location of target site | 3'UTR | ||||||
| Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
| Original Description (Extracted from the article) |
...
PAR-CLIP data was present in GSM545216. RNA binding protein: AGO2. Condition:miR-124 transfection
... - Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell. |
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| miRNA-target interactions (Provided by authors) |
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| Article |
- Hafner M; Landthaler M; Burger L; Khorshid et al. - Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
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| Experimental Support 2 for Functional miRNA-Target Interaction | |
|---|---|
| miRNA:Target | ---- |
| Validation Method |
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| Conditions | HEK293 |
| Disease | 261726.0 |
| Location of target site | 3'UTR |
| Tools used in this research | TargetScan , miRTarCLIP , Piranha |
| Original Description (Extracted from the article) |
...
"PAR-CLIP data was present in GSM1065668. RNA binding protein: AGO1. Condition:4-thiouridine
"PAR-CLIP data was present in GSM1065670. RNA binding protein: AGO2. Condition:4-thiouridine
... - Memczak S; Jens M; Elefsinioti A; Torti F; et al., 2013, Nature. |
| Article |
- Memczak S; Jens M; Elefsinioti A; Torti F; et al. - Nature, 2013
Circular RNAs (circRNAs) in animals are an enigmatic class of RNA with unknown function. To explore circRNAs systematically, we sequenced and computationally analysed human, mouse and nematode RNA. We detected thousands of well-expressed, stable circRNAs, often showing tissue/developmental-stage-specific expression. Sequence analysis indicated important regulatory functions for circRNAs. We found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7. Further analyses indicated that CDR1as functions to bind miR-7 in neuronal tissues. Human CDR1as expression in zebrafish impaired midbrain development, similar to knocking down miR-7, suggesting that CDR1as is a miRNA antagonist with a miRNA-binding capacity ten times higher than any other known transcript. Together, our data provide evidence that circRNAs form a large class of post-transcriptional regulators. Numerous circRNAs form by head-to-tail splicing of exons, suggesting previously unrecognized regulatory potential of coding sequences.
LinkOut: [PMID: 23446348]
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| CLIP-seq Support 1 for dataset GSM545216 | |
|---|---|
| Method / RBP | PAR-CLIP / AGO2 |
| Cell line / Condition | HEK293 / miR-124 transfection |
| Location of target site | ENST00000367833.2 | 3UTR | AUACAACAUAUACUCACUAUG |
| Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
| Article / Accession Series | PMID: 20371350 / GSE21578 |
| CLIP-seq Viewer | Link |
| CLIP-seq Support 2 for dataset GSM1065668 | |
|---|---|
| Method / RBP | PAR-CLIP / AGO1 |
| Cell line / Condition | HEK293 / 4-thiouridine, ML_MM_7 |
| Location of target site | ENST00000367833.2 | 3UTR | AUACAACAUAUACUCACUAUG |
| Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
| Article / Accession Series | PMID: 23446348 / GSE43573 |
| CLIP-seq Viewer | Link |
| CLIP-seq Support 3 for dataset GSM1065670 | |
|---|---|
| Method / RBP | PAR-CLIP / AGO2 |
| Cell line / Condition | HEK293 / 4-thiouridine, 3_ML_LG |
| Location of target site | ENST00000367833.2 | 3UTR | AUACAACAUAUACUCACUAUG |
| Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
| Article / Accession Series | PMID: 23446348 / GSE43573 |
| CLIP-seq Viewer | Link |
| MiRNA-Target Expression Profile | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| MiRNA-Target Expression Profile (TCGA) | |||||||
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63 hsa-miR-921 Target Genes:
Functional analysis:
ID |
Target | Description | Validation methods |
 |
 |
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| Strong evidence | Less strong evidence | |||||||||||
 |
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 |
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| MIRT054764 | ANGPTL1 | angiopoietin like 1 |  |
|
|
3 | 1 | |||||
| MIRT066171 | PIP4K2C | phosphatidylinositol-5-phosphate 4-kinase type 2 gamma | |
|
2 | 2 | ||||||
| MIRT069409 | ZFYVE21 | zinc finger FYVE-type containing 21 | |
|
2 | 8 | ||||||
| MIRT102284 | DNAJB9 | DnaJ heat shock protein family (Hsp40) member B9 | |
|
2 | 4 | ||||||
| MIRT107595 | DNAJA1 | DnaJ heat shock protein family (Hsp40) member A1 | |
|
2 | 6 | ||||||
| MIRT178618 | HIAT1 | major facilitator superfamily domain containing 14A | |
|
2 | 2 | ||||||
| MIRT182407 | TIPRL | TOR signaling pathway regulator | |
|
2 | 4 | ||||||
| MIRT186552 | ZBTB18 | zinc finger and BTB domain containing 18 | |
|
2 | 2 | ||||||
| MIRT273662 | HOXC8 | homeobox C8 | |
|
2 | 2 | ||||||
| MIRT283191 | C16ORF52 | chromosome 16 open reading frame 52 | |
|
2 | 2 | ||||||
| MIRT284890 | NFAT5 | nuclear factor of activated T-cells 5 | |
|
2 | 2 | ||||||
| MIRT347670 | LSM14A | LSM14A, mRNA processing body assembly factor | |
|
2 | 2 | ||||||
| MIRT400222 | SLC35F6 | solute carrier family 35 member F6 | |
|
2 | 2 | ||||||
| MIRT403517 | ASPH | aspartate beta-hydroxylase | |
|
2 | 2 | ||||||
| MIRT442251 | DCTN5 | dynactin subunit 5 | |
|
2 | 2 | ||||||
| MIRT443023 | SDR39U1 | short chain dehydrogenase/reductase family 39U member 1 | |
|
2 | 2 | ||||||
| MIRT443097 | RNF20 | ring finger protein 20 | |
|
2 | 2 | ||||||
| MIRT444560 | TRA2B | transformer 2 beta homolog | |
|
2 | 2 | ||||||
| MIRT445696 | PRKG1 | protein kinase, cGMP-dependent, type I | |
|
2 | 2 | ||||||
| MIRT454084 | TMEM209 | transmembrane protein 209 | |
|
2 | 2 | ||||||
| MIRT455463 | LYPLA2 | lysophospholipase II | |
|
2 | 2 | ||||||
| MIRT456653 | TIFA | TRAF interacting protein with forkhead associated domain | |
|
2 | 2 | ||||||
| MIRT458147 | LYRM4 | LYR motif containing 4 | |
|
2 | 6 | ||||||
| MIRT467073 | SRRD | SRR1 domain containing | |
|
2 | 4 | ||||||
| MIRT467245 | SPPL2A | signal peptide peptidase like 2A | |
|
2 | 2 | ||||||
| MIRT468246 | SFXN4 | sideroflexin 4 | |
|
2 | 2 | ||||||
| MIRT471589 | PAQR5 | progestin and adipoQ receptor family member 5 | |
|
2 | 19 | ||||||
| MIRT476639 | G2E3 | G2/M-phase specific E3 ubiquitin protein ligase | |
|
2 | 2 | ||||||
| MIRT482433 | ADM | adrenomedullin | |
|
2 | 10 | ||||||
| MIRT486848 | PERP | PERP, TP53 apoptosis effector | |
|
2 | 6 | ||||||
| MIRT489656 | SHMT1 | serine hydroxymethyltransferase 1 | |
|
2 | 2 | ||||||
| MIRT493441 | KANSL1 | KAT8 regulatory NSL complex subunit 1 | |
|
2 | 6 | ||||||
| MIRT493841 | FOXN3 | forkhead box N3 | |
|
2 | 4 | ||||||
| MIRT501378 | RBFOX2 | RNA binding protein, fox-1 homolog 2 | |
|
2 | 10 | ||||||
| MIRT509679 | ATAD5 | ATPase family, AAA domain containing 5 | |
|
2 | 4 | ||||||
| MIRT510280 | MED28 | mediator complex subunit 28 | |
|
2 | 2 | ||||||
| MIRT512221 | ATXN3 | ataxin 3 | |
|
2 | 6 | ||||||
| MIRT514030 | BNIP2 | BCL2 interacting protein 2 | |
|
2 | 2 | ||||||
| MIRT521375 | RDX | radixin | |
|
2 | 4 | ||||||
| MIRT521444 | RAD51 | RAD51 recombinase | |
|
2 | 2 | ||||||
| MIRT526055 | CBR1 | carbonyl reductase 1 | |
|
2 | 2 | ||||||
| MIRT528658 | FUNDC2 | FUN14 domain containing 2 | |
|
2 | 2 | ||||||
| MIRT529975 | TNFAIP8L1 | TNF alpha induced protein 8 like 1 | |
|
2 | 2 | ||||||
| MIRT544098 | IPMK | inositol polyphosphate multikinase | |
|
2 | 2 | ||||||
| MIRT545579 | SNRPA1 | small nuclear ribonucleoprotein polypeptide A' | |
|
2 | 2 | ||||||
| MIRT547424 | MED4 | mediator complex subunit 4 | |
|
2 | 2 | ||||||
| MIRT548955 | CD2AP | CD2 associated protein | |
|
2 | 2 | ||||||
| MIRT549537 | NDUFA6 | NADH:ubiquinone oxidoreductase subunit A6 | |
|
2 | 4 | ||||||
| MIRT552550 | ZFP36L2 | ZFP36 ring finger protein like 2 | |
|
2 | 4 | ||||||
| MIRT554640 | ROBO1 | roundabout guidance receptor 1 | |
|
2 | 2 | ||||||
| MIRT564904 | YTHDF1 | YTH N6-methyladenosine RNA binding protein 1 | |
|
2 | 2 | ||||||
| MIRT565578 | SLC6A8 | solute carrier family 6 member 8 | |
|
2 | 2 | ||||||
| MIRT568312 | BAG4 | BCL2 associated athanogene 4 | |
|
2 | 2 | ||||||
| MIRT617891 | PTCHD3 | patched domain containing 3 | |
|
2 | 2 | ||||||
| MIRT621892 | TAF13 | TATA-box binding protein associated factor 13 | |
|
2 | 2 | ||||||
| MIRT642850 | RNF135 | ring finger protein 135 | |
|
2 | 2 | ||||||
| MIRT665395 | WEE1 | WEE1 G2 checkpoint kinase | |
|
2 | 2 | ||||||
| MIRT697879 | UBE2B | ubiquitin conjugating enzyme E2 B | |
|
2 | 2 | ||||||
| MIRT698492 | THOC2 | THO complex 2 | |
|
2 | 2 | ||||||
| MIRT701227 | OCRL | OCRL, inositol polyphosphate-5-phosphatase | |
|
2 | 2 | ||||||
| MIRT701872 | MPLKIP | M-phase specific PLK1 interacting protein | |
|
2 | 2 | ||||||
| MIRT707045 | TRPV2 | transient receptor potential cation channel subfamily V member 2 | |
|
2 | 2 | ||||||
| MIRT715216 | NPVF | neuropeptide VF precursor | |
|
2 | 2 | ||||||
| miRNA-Drug Associations | |||||||||||||||||||||||||||
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| miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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