miRTarBase - #MIRT260611


—
pre-miRNA Information
pre-miRNA hsa-mir-3529   
Genomic Coordinates chr15: 88611847 - 88611924
Description Homo sapiens miR-3529 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-3529-3p
Sequence 46| AACAACAAAAUCACUAGUCUUCCA |69
Evidence Not_experimental
Experiments
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1196555927 1 dbSNP
rs1215541761 2 dbSNP
rs761252497 3 dbSNP
rs1263337637 4 dbSNP
rs868026301 6 dbSNP
rs762762175 9 dbSNP
rs751269230 14 dbSNP
rs1257397051 16 dbSNP
Putative Targets

Gene Information
Gene Symbol SOX4   
Synonyms EVI16
Description SRY-box 4
Transcript NM_003107   
Expression
Putative miRNA Targets on SOX4
3'UTR of SOX4
(miRNA target sites are highlighted)		 
>SOX4|NM_003107|3'UTR
   1 AGGGCGCGCAGGCAGGGAGAAGGGCCGGGGGGGGTAGGAGAGGAGAAAAAAAAAGTGAAAAAAAGAAACGAAAAGGACAG
  81 ACGAAGAGTTTAAAGAGAAAAGGGAAAAAAGAAAGAAAAAGTAAGCAGGGCTGGCTTCGCCCGCGTTCTCGTCGTCGGAT
 161 CAAGGAGCGCGGCGGCGTTTTGGACCCGCGCTCCCATCCCCCACCTTCCCGGGCCGGGGACCCACTCTGCCCAGCCGGAG
 241 GGACGCGGAGGAGGAAGAGGGTAGACAGGGGCGACCTGTGATTGTTGTTATTGATGTTGTTGTTGATGGCAAAAAAAAAA
 321 AAGCGACTTCGAGTTTGCTCCCCTTTGCTTGAAGAGACCCCCTCCCCCTTCCAACGAGCTTCCGGACTTGTCTGCACCCC
 401 CAGCAAGAAGGCGAGTTAGTTTTCTAGAGACTTGAAGGAGTCTCCCCCTTCCTGCATCACCACCTTGGTTTTGTTTTATT
 481 TTGCTTCTTGGTCAAGAAAGGAGGGGAGAACCCAGCGCACCCCTCCCCCCCTTTTTTTAAACGCGTGATGAAGACAGAAG
 561 GCTCCGGGGTGACGAATTTGGCCGATGGCAGATGTTTTGGGGGAACGCCGGGACTGAGAGACTCCACGCAGGCGAATTCC
 641 CGTTTGGGGCTTTTTTTTCCTCCCTCTTTTCCCCTTGCCCCCTCTGCAGCCGGAGGAGGAGATGTTGAGGGGAGGAGGCC
 721 AGCCAGTGTGACCGGCGCTAGGAAATGACCCGAGAACCCCGTTGGAAGCGCAGCAGCGGGAGCTAGGGGCGGGGGCGGAG
 801 GAGGACACGAACTGGAAGGGGGTTCACGGTCAAACTGAAATGGATTTGCACGTTGGGGAGCTGGCGGCGGCGGCTGCTGG
 881 GCCTCCGCCTTCTTTTCTACGTGAAATCAGTGAGGTGAGACTTCCCAGACCCCGGAGGCGTGGAGGAGAGGAGACTGTTT
 961 GATGTGGTACAGGGGCAGTCAGTGGAGGGCGAGTGGTTTCGGAAAAAAAAAAAGAAAAAAAGAAAAAAAAAGAAAAAAAA
1041 AAGATTTTTTTCTTCTCTTAATCGGAATCGTGATGGTGTTGGATTATTTCAATGGTGGGGTTAATATAGCATGTTATCCT
1121 GTCTATCTTTTAAAGATTTCTGTATAAGACTGTTGAGCAGTTTTTAAAATAGTGTAGGATAATATAAAAAGCAGATAGAT
1201 GGCGCTATGTTTGATTCCTACAACGAAATTATCACCAGCTTTTTTTCATTCTTAACTCTTTAAAGGATTCAAACGCAACT
1281 CAAATCTGTGCTGGACTTTAAAAAAACAATTCAGGACCAAATTTTTTCTCAGTGTGTGTGTTTATTCCTTATAGGTGTAA
1361 ATGAGAAGACGTGTTTTTTTCCTTCACCGATGCTCCATCCTCGTATTTCTTTTTCCTTGTAAATGTAATCAGATGCCATT
1441 TTATATGTGGACGTATTTATACTGGCCAAACATATTTTTTCTTTTGTCCCTTTTTTTCTTTCCTTTCTTTTTACTTCCTT
1521 TATTTCTTTATTCCTTCCTTTTCCTTTTTTTCTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTGGTAGTTGTTGTTACCCA
1601 CGCCATTTTACGTCTCCTTCACTGAAGGGCTAGAGTTTTAACTTTTAATTTTTTATATTTAAATGTAGACTTTTGACACT
1681 TTTAAAAAACAAAAAAAGACAAGAGAGATGAAAACGTTTGATTATTTTCTCAGTGTATTTTTGTAAAAAATATATAAAGG
1761 GGGTGTTAATCGGTGTAAATCGCTGTTTGGATTTCCTGATTTTATAACAGGGCGGCTGGTTAATATCTCACACAGTTTAA
1841 AAAATCAGCCCCTAATTTCTCCATGTTTACACTTCAATCTGCAGGCTTCTTAAAGTGACAGTATCCCTTAACCTGCCACC
1921 AGTGTCCACCCTCCGGCCCCCGTCTTGTAAAAAGGGGAGGAGAATTAGCCAAACACTGTAAGCTTTTAAGAAAAACAAAG
2001 TTTTAAACGAAATACTGCTCTGTCCAGAGGCTTTAAAACTGGTGCAATTACAGCAAAAAGGGATTCTGTAGCTTTAACTT
2081 GTAAACCACATCTTTTTTGCACTTTTTTTATAAGCAAAAACGTGCCGTTTAAACCACTGGATCTATCTAAATGCCGATTT
2161 GAGTTCGCGACACTATGTACTGCGTTTTTCATTCTTGTATTTGACTATTTAATCCTTTCTACTTGTCGCTAAATATAATT
2241 GTTTTAGTCTTATGGCATGATGATAGCATATGTGTTCAGGTTTATAGCTGTTGTGTTTAAAAATTGAAAAAAGTGGAAAA
2321 CATCTTTGTACATTTAAGTCTGTATTATAATAAGCAAAAAGATTGTGTGTATGTATGTTTAATATAACATGACAGGCACT
2401 AGGACGTCTGCCTTTTTAAGGCAGTTCCGTTAAGGGTTTTTGTTTTTAAACTTTTTTTTGCCATCCATCCTGTGCAATAT
2481 GCCGTGTAGAATATTTGTCTTAAAATTCAAGGCCACAAAAACAATGTTTGGGGGAAAAAAAAGAAAAAATCATGCCAGCT
2561 AATCATGTCAAGTTCACTGCCTGTCAGATTGTTGATATATACCTTCTGTAAATAACTTTTTTTGAGAAGGAAATAAAATC
2641 AGCTGGAACTGAACCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' accuucUGAUCACUAAAACAACAa 5'
                ::|| |||| ||||||| 
Target 5' attgttGTTATTGATGTTGTTGTt 3'
 281 - 304 166.00 -10.60
2 
miRNA  3' accuucugaucACUA-AAACAACAa 5'
                     ||:|  ||||||| 
Target 5' tttttttttttTGGTAGTTGTTGTt 3'
 1571 - 1595 145.00 -8.40
3 
miRNA  3' accUUC---UGAU---CACUAAAACAACaa 5'
             |||   |||:   || |  ||||||  
Target 5' gtcAAGTTCACTGCCTGTCAGATTGTTGat 3'
 2567 - 2596 133.00 -6.50
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN20102460 35 COSMIC
COSN30130578 41 COSMIC
COSN32051869 55 COSMIC
COSN31562045 65 COSMIC
COSN1329761 88 COSMIC
COSN30138601 110 COSMIC
COSN31515459 112 COSMIC
COSN26578619 122 COSMIC
COSN31549936 132 COSMIC
COSN30110570 146 COSMIC
COSN30165627 154 COSMIC
COSN9776930 173 COSMIC
COSN31480191 228 COSMIC
COSN30529869 316 COSMIC
COSN1329762 323 COSMIC
COSN27346377 323 COSMIC
COSN7866970 349 COSMIC
COSN26957634 444 COSMIC
COSN31555263 533 COSMIC
COSN9776931 571 COSMIC
COSN31589084 597 COSMIC
COSN30143967 628 COSMIC
COSN31538414 659 COSMIC
COSN15626430 667 COSMIC
COSN6315496 780 COSMIC
COSN19660705 809 COSMIC
COSN30116000 834 COSMIC
COSN31557315 869 COSMIC
COSN1329763 913 COSMIC
COSN31488402 947 COSMIC
COSN31488648 952 COSMIC
COSN27459301 1014 COSMIC
COSN17153537 1015 COSMIC
COSN16531558 1033 COSMIC
COSN17401069 1035 COSMIC
COSN31563627 1043 COSMIC
COSN30529921 1044 COSMIC
COSN31516477 1045 COSMIC
COSN31606864 1056 COSMIC
COSN31487056 1215 COSMIC
COSN31580128 1307 COSMIC
COSN15626431 1331 COSMIC
COSN26958475 1331 COSMIC
COSN26958476 1332 COSMIC
COSN30132757 1491 COSMIC
COSN20547843 1504 COSMIC
COSN20545020 1508 COSMIC
COSN31570653 1510 COSMIC
COSN30170329 1535 COSMIC
COSN31539620 1545 COSMIC
COSN8513792 1553 COSMIC
COSN30175895 1563 COSMIC
COSN20390156 1564 COSMIC
COSN8513793 1572 COSMIC
COSN30140151 1602 COSMIC
COSN30510670 1615 COSMIC
COSN26465215 1643 COSMIC
COSN30537787 1683 COSMIC
COSN30537790 1684 COSMIC
COSN25100621 1725 COSMIC
COSN30124593 1751 COSMIC
COSN30529475 1792 COSMIC
COSN31537778 1814 COSMIC
COSN26554556 1815 COSMIC
COSN30130802 1827 COSMIC
COSN15626432 1906 COSMIC
COSN31610520 1945 COSMIC
COSN15626433 1955 COSMIC
COSN31516778 1972 COSMIC
COSN30113527 2049 COSMIC
COSN26791827 2098 COSMIC
COSN31595308 2104 COSMIC
COSN30533797 2155 COSMIC
COSN28669879 2182 COSMIC
COSN30512136 2199 COSMIC
COSN30118164 2248 COSMIC
COSN15966676 2272 COSMIC
COSN30533494 2310 COSMIC
COSN31589997 2329 COSMIC
COSN30136042 2344 COSMIC
COSN30113605 2362 COSMIC
COSN30173879 2366 COSMIC
COSN30530925 2436 COSMIC
COSN26958477 2527 COSMIC
COSN23012864 2535 COSMIC
COSN31551638 2536 COSMIC
COSN8513794 2549 COSMIC
COSN28669330 2598 COSMIC
COSN2499110 2647 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs758667509 3 dbSNP
rs777809842 5 dbSNP
rs747430686 6 dbSNP
rs1452161223 7 dbSNP
rs1180814300 8 dbSNP
rs1236140480 10 dbSNP
rs1441095212 11 dbSNP
rs1178890231 13 dbSNP
rs923920469 16 dbSNP
rs1384031165 18 dbSNP
rs958354281 23 dbSNP
rs1164194102 26 dbSNP
rs1164541918 26 dbSNP
rs764320667 27 dbSNP
rs774787329 27 dbSNP
rs1392063410 28 dbSNP
rs1409968157 29 dbSNP
rs781728088 30 dbSNP
rs768224472 31 dbSNP
rs1270229207 32 dbSNP
rs200088857 32 dbSNP
rs760527295 33 dbSNP
rs113141237 34 dbSNP
rs150449012 34 dbSNP
rs1247059117 35 dbSNP
rs1481268683 36 dbSNP
rs1177086723 38 dbSNP
rs765453290 39 dbSNP
rs1407076067 41 dbSNP
rs752846533 42 dbSNP
rs767852291 43 dbSNP
rs758368209 44 dbSNP
rs1406645806 46 dbSNP
rs199766530 46 dbSNP
rs754437511 46 dbSNP
rs764379585 47 dbSNP
rs1345246005 49 dbSNP
rs1199078075 50 dbSNP
rs1480236204 51 dbSNP
rs1397482308 55 dbSNP
rs11413405 57 dbSNP
rs534670825 57 dbSNP
rs546083928 57 dbSNP
rs1046571107 60 dbSNP
rs1025265961 62 dbSNP
rs1051079 63 dbSNP
rs1203175941 64 dbSNP
rs1437962292 66 dbSNP
rs546528035 67 dbSNP
rs534834455 69 dbSNP
rs553929047 70 dbSNP
rs1220782711 73 dbSNP
rs1306301588 81 dbSNP
rs888100184 85 dbSNP
rs571459101 88 dbSNP
rs1402470749 89 dbSNP
rs1334802149 93 dbSNP
rs1469438770 96 dbSNP
rs766429313 96 dbSNP
rs868578396 98 dbSNP
rs1178994195 99 dbSNP
rs1399366211 101 dbSNP
rs1005139325 102 dbSNP
rs1039600916 103 dbSNP
rs1178351760 104 dbSNP
rs1051048 105 dbSNP
rs977303692 105 dbSNP
rs899738469 106 dbSNP
rs1181253556 107 dbSNP
rs1460578738 108 dbSNP
rs755789293 108 dbSNP
rs1314978704 110 dbSNP
rs943856841 110 dbSNP
rs16901614 112 dbSNP
rs777230863 114 dbSNP
rs1048518278 116 dbSNP
rs931349428 116 dbSNP
rs1436742788 118 dbSNP
rs372757653 121 dbSNP
rs1452057319 123 dbSNP
rs1254785239 124 dbSNP
rs1467137643 125 dbSNP
rs79958549 126 dbSNP
rs1046668416 127 dbSNP
rs1160322065 130 dbSNP
rs906755544 130 dbSNP
rs1002886917 133 dbSNP
rs1051049 134 dbSNP
rs954842684 135 dbSNP
rs1161112892 137 dbSNP
rs1443544811 139 dbSNP
rs1000016194 140 dbSNP
rs557312513 141 dbSNP
rs1166405097 145 dbSNP
rs1418835384 146 dbSNP
rs1355727233 149 dbSNP
rs575573467 151 dbSNP
rs1245316817 155 dbSNP
rs1314943524 158 dbSNP
rs113534734 160 dbSNP
rs1361568749 161 dbSNP
rs537058170 162 dbSNP
rs958026636 163 dbSNP
rs555683849 166 dbSNP
rs554443753 174 dbSNP
rs1321552950 182 dbSNP
rs966998565 186 dbSNP
rs1166323489 187 dbSNP
rs1426323911 191 dbSNP
rs1418398751 195 dbSNP
rs1328589377 197 dbSNP
rs989457753 212 dbSNP
rs916846964 213 dbSNP
rs977087436 218 dbSNP
rs969614076 222 dbSNP
rs1266673918 225 dbSNP
rs982324079 226 dbSNP
rs1225391585 236 dbSNP
rs1338394871 236 dbSNP
rs1019713141 237 dbSNP
rs928118167 241 dbSNP
rs929773230 242 dbSNP
rs1233363423 244 dbSNP
rs1343953903 245 dbSNP
rs769412942 247 dbSNP
rs1283057912 259 dbSNP
rs1406038872 260 dbSNP
rs574096785 264 dbSNP
rs528494731 267 dbSNP
rs1460068826 268 dbSNP
rs1212996617 270 dbSNP
rs1478598695 271 dbSNP
rs1262119729 276 dbSNP
rs965303767 277 dbSNP
rs1190823429 280 dbSNP
rs1241738977 282 dbSNP
rs1251971355 289 dbSNP
rs1475690237 289 dbSNP
rs1200695635 293 dbSNP
rs1167121412 294 dbSNP
rs975740716 295 dbSNP
rs921073172 298 dbSNP
rs111640879 306 dbSNP
rs1333309679 308 dbSNP
rs113192344 311 dbSNP
rs1466816735 311 dbSNP
rs796555280 311 dbSNP
rs931169401 311 dbSNP
rs984203067 311 dbSNP
rs1175419487 313 dbSNP
rs1466411532 315 dbSNP
rs559729519 317 dbSNP
rs1179492584 322 dbSNP
rs940099564 323 dbSNP
rs1159672487 324 dbSNP
rs1387833314 325 dbSNP
rs1380853648 326 dbSNP
rs1318160641 331 dbSNP
rs1046368530 332 dbSNP
rs982944204 339 dbSNP
rs1339433622 341 dbSNP
rs909595390 342 dbSNP
rs759964109 343 dbSNP
rs1415038009 344 dbSNP
rs1294521557 348 dbSNP
rs867957273 349 dbSNP
rs1262804211 352 dbSNP
rs1353056147 354 dbSNP
rs1347658775 358 dbSNP
rs938242821 358 dbSNP
rs1238886787 359 dbSNP
rs1261219898 361 dbSNP
rs1055817164 362 dbSNP
rs893970386 363 dbSNP
rs1203145235 364 dbSNP
rs1283309459 365 dbSNP
rs941051447 370 dbSNP
rs1011052112 372 dbSNP
rs1039316668 375 dbSNP
rs1452924148 377 dbSNP
rs899791145 383 dbSNP
rs1190364172 385 dbSNP
rs1021315495 386 dbSNP
rs1442915374 388 dbSNP
rs902914424 393 dbSNP
rs577792688 397 dbSNP
rs752534861 397 dbSNP
rs765655839 398 dbSNP
rs903007596 403 dbSNP
rs998665711 407 dbSNP
rs1031521783 408 dbSNP
rs753133052 415 dbSNP
rs975349166 420 dbSNP
rs1356559323 424 dbSNP
rs1313759656 425 dbSNP
rs1028698780 426 dbSNP
rs1452333071 427 dbSNP
rs1010940299 428 dbSNP
rs952507311 439 dbSNP
rs1300028670 441 dbSNP
rs1422670181 441 dbSNP
rs983984884 442 dbSNP
rs550752789 443 dbSNP
rs940047325 443 dbSNP
rs545170892 444 dbSNP
rs563656230 446 dbSNP
rs77177818 449 dbSNP
rs113778405 450 dbSNP
rs1490337429 451 dbSNP
rs1288483163 455 dbSNP
rs1035242103 456 dbSNP
rs1307155864 458 dbSNP
rs1209959610 459 dbSNP
rs951631146 460 dbSNP
rs758882255 461 dbSNP
rs982949753 464 dbSNP
rs1279868807 466 dbSNP
rs1324247440 468 dbSNP
rs1364917156 468 dbSNP
rs909650551 468 dbSNP
rs902695198 470 dbSNP
rs998583723 474 dbSNP
rs941103547 487 dbSNP
rs1041227588 488 dbSNP
rs1167489831 488 dbSNP
rs1426172314 489 dbSNP
rs560939044 491 dbSNP
rs1480380081 494 dbSNP
rs1247856253 496 dbSNP
rs1221642196 499 dbSNP
rs1452896422 500 dbSNP
rs996940564 501 dbSNP
rs920928057 506 dbSNP
rs1485504823 507 dbSNP
rs933944962 509 dbSNP
rs1259706183 510 dbSNP
rs1221501768 512 dbSNP
rs1366789644 513 dbSNP
rs1051462037 517 dbSNP
rs1196444986 522 dbSNP
rs903075517 523 dbSNP
rs1275436384 524 dbSNP
rs1005474844 525 dbSNP
rs1438530515 525 dbSNP
rs1328297853 526 dbSNP
rs1164065142 527 dbSNP
rs144133444 529 dbSNP
rs184361355 530 dbSNP
rs571497565 531 dbSNP
rs1230623889 532 dbSNP
rs1455838637 532 dbSNP
rs1457910379 532 dbSNP
rs1010991158 533 dbSNP
rs959386487 534 dbSNP
rs1023673115 542 dbSNP
rs1279084448 547 dbSNP
rs1220828291 554 dbSNP
rs538850161 558 dbSNP
rs905200595 559 dbSNP
rs756775253 562 dbSNP
rs550752310 563 dbSNP
rs1452194496 564 dbSNP
rs1360048070 566 dbSNP
rs187509815 567 dbSNP
rs1399422198 569 dbSNP
rs1407943938 570 dbSNP
rs780578157 575 dbSNP
rs1042629301 579 dbSNP
rs146898567 584 dbSNP
rs962474548 591 dbSNP
rs944908444 600 dbSNP
rs555519149 601 dbSNP
rs745780496 607 dbSNP
rs1371689560 610 dbSNP
rs192477325 611 dbSNP
rs1212027588 615 dbSNP
rs1458295308 615 dbSNP
rs1445190920 616 dbSNP
rs1285296319 621 dbSNP
rs1040781230 623 dbSNP
rs1355857384 625 dbSNP
rs1313025681 626 dbSNP
rs920980422 627 dbSNP
rs769645048 628 dbSNP
rs1304926741 642 dbSNP
rs996623541 643 dbSNP
rs1360432289 650 dbSNP
rs1299412810 651 dbSNP
rs1422077040 651 dbSNP
rs1381576867 654 dbSNP
rs534834538 660 dbSNP
rs986453258 661 dbSNP
rs888118713 663 dbSNP
rs1005462017 664 dbSNP
rs1254145606 665 dbSNP
rs774099474 667 dbSNP
rs924551350 672 dbSNP
rs780018737 678 dbSNP
rs1192077160 679 dbSNP
rs1394859115 680 dbSNP
rs1432191424 683 dbSNP
rs934576960 685 dbSNP
rs1374484530 688 dbSNP
rs1459997865 690 dbSNP
rs1015558536 694 dbSNP
rs36017767 695 dbSNP
rs1340230061 700 dbSNP
rs1279259571 705 dbSNP
rs1054329161 707 dbSNP
rs914397839 708 dbSNP
rs1364296918 711 dbSNP
rs948498544 713 dbSNP
rs1443032905 716 dbSNP
rs1003814845 717 dbSNP
rs1045174326 717 dbSNP
rs905252885 719 dbSNP
rs1003968598 720 dbSNP
rs553071938 724 dbSNP
rs959699244 725 dbSNP
rs1056457532 726 dbSNP
rs768506235 727 dbSNP
rs887382525 734 dbSNP
rs990923684 735 dbSNP
rs1004467409 748 dbSNP
rs915208826 751 dbSNP
rs1297972528 758 dbSNP
rs1017092140 759 dbSNP
rs768256578 761 dbSNP
rs1259074274 772 dbSNP
rs900150139 772 dbSNP
rs773411748 775 dbSNP
rs577682127 777 dbSNP
rs996931862 778 dbSNP
rs1273902344 782 dbSNP
rs545207747 784 dbSNP
rs976629177 785 dbSNP
rs1028048525 786 dbSNP
rs760814494 788 dbSNP
rs766331342 789 dbSNP
rs557086958 791 dbSNP
rs956183175 794 dbSNP
rs1375853844 795 dbSNP
rs575582555 796 dbSNP
rs139085828 798 dbSNP
rs941029578 799 dbSNP
rs1188699776 809 dbSNP
rs1263402550 813 dbSNP
rs1457468000 816 dbSNP
rs1239084059 818 dbSNP
rs914451582 820 dbSNP
rs1321773854 822 dbSNP
rs1255788517 823 dbSNP
rs1234540588 826 dbSNP
rs1314493303 827 dbSNP
rs897033361 828 dbSNP
rs948551941 829 dbSNP
rs1035228365 830 dbSNP
rs1331699708 836 dbSNP
rs560757796 838 dbSNP
rs776604025 844 dbSNP
rs926730357 852 dbSNP
rs939435797 853 dbSNP
rs1399720208 858 dbSNP
rs1302078887 861 dbSNP
rs1417957941 863 dbSNP
rs1462914052 866 dbSNP
rs894987997 870 dbSNP
rs1012278723 872 dbSNP
rs1180686861 873 dbSNP
rs1349281025 873 dbSNP
rs1440927658 876 dbSNP
rs1022375062 878 dbSNP
rs1201426399 886 dbSNP
rs1056910249 890 dbSNP
rs575602892 891 dbSNP
rs543027975 892 dbSNP
rs1237317409 893 dbSNP
rs1360120115 893 dbSNP
rs1352250907 895 dbSNP
rs1030975712 899 dbSNP
rs564979921 900 dbSNP
rs1293939252 902 dbSNP
rs1338377953 905 dbSNP
rs898728223 908 dbSNP
rs1333238540 909 dbSNP
rs996998890 910 dbSNP
rs1357465978 913 dbSNP
rs1404266433 915 dbSNP
rs747178026 915 dbSNP
rs1419039079 925 dbSNP
rs532195982 931 dbSNP
rs753183806 932 dbSNP
rs1368161037 934 dbSNP
rs1007968881 935 dbSNP
rs558160482 942 dbSNP
rs1031801646 943 dbSNP
rs1261482324 944 dbSNP
rs932205901 945 dbSNP
rs1488915965 947 dbSNP
rs1259346761 950 dbSNP
rs550837670 951 dbSNP
rs763401964 952 dbSNP
rs1245077064 954 dbSNP
rs1341984248 955 dbSNP
rs1276101142 956 dbSNP
rs1487067167 956 dbSNP
rs985189020 956 dbSNP
rs758826683 958 dbSNP
rs1192526094 962 dbSNP
rs1403730937 968 dbSNP
rs569063799 971 dbSNP
rs1163983617 972 dbSNP
rs1459653416 976 dbSNP
rs1475890080 977 dbSNP
rs1421872024 978 dbSNP
rs1193447803 986 dbSNP
rs1478422428 988 dbSNP
rs1266049443 990 dbSNP
rs1185216181 991 dbSNP
rs941101618 993 dbSNP
rs1449310682 995 dbSNP
rs1412975516 1000 dbSNP
rs1210017014 1001 dbSNP
rs3209850 1002 dbSNP
rs1333344324 1003 dbSNP
rs3209851 1003 dbSNP
rs529860272 1003 dbSNP
rs573157025 1003 dbSNP
rs1434216842 1004 dbSNP
rs1419663176 1005 dbSNP
rs1324307198 1006 dbSNP
rs1398437677 1007 dbSNP
rs879078009 1007 dbSNP
rs1160929768 1011 dbSNP
rs955733223 1013 dbSNP
rs1170393098 1014 dbSNP
rs1171282012 1015 dbSNP
rs1245588810 1015 dbSNP
rs1426241795 1015 dbSNP
rs1466532949 1015 dbSNP
rs1478002478 1015 dbSNP
rs896822535 1017 dbSNP
rs1469513982 1018 dbSNP
rs1343244631 1019 dbSNP
rs1197770749 1020 dbSNP
rs1274871341 1022 dbSNP
rs1318247291 1022 dbSNP
rs1217357350 1023 dbSNP
rs1236665811 1023 dbSNP
rs1275491007 1023 dbSNP
rs1344258606 1023 dbSNP
rs1374951474 1023 dbSNP
rs989790532 1025 dbSNP
rs372058314 1026 dbSNP
rs1447815417 1027 dbSNP
rs1402851562 1028 dbSNP
rs1332648988 1029 dbSNP
rs1414376488 1029 dbSNP
rs1313684713 1030 dbSNP
rs939232510 1030 dbSNP
rs1377542649 1031 dbSNP
rs1377949960 1032 dbSNP
rs1021563792 1033 dbSNP
rs1056419187 1033 dbSNP
rs1207804235 1033 dbSNP
rs1279331282 1033 dbSNP
rs1457950185 1033 dbSNP
rs796750510 1033 dbSNP
rs969952419 1039 dbSNP
rs1012061187 1041 dbSNP
rs894969421 1041 dbSNP
rs1022325807 1042 dbSNP
rs1241098871 1044 dbSNP
rs1282769145 1044 dbSNP
rs1447830264 1045 dbSNP
rs979957778 1045 dbSNP
rs142990183 1047 dbSNP
rs999585326 1051 dbSNP
rs1358016510 1052 dbSNP
rs1374611823 1053 dbSNP
rs1409783451 1056 dbSNP
rs939468203 1056 dbSNP
rs1225982152 1057 dbSNP
rs1305260076 1058 dbSNP
rs1472305903 1064 dbSNP
rs1237856261 1067 dbSNP
rs1212877828 1068 dbSNP
rs1353467538 1077 dbSNP
rs1487287924 1083 dbSNP
rs1212108385 1084 dbSNP
rs1285364312 1086 dbSNP
rs1280563541 1093 dbSNP
rs1217505813 1099 dbSNP
rs1031384363 1100 dbSNP
rs966103446 1108 dbSNP
rs1227961818 1110 dbSNP
rs1380272097 1119 dbSNP
rs992263966 1120 dbSNP
rs1486488618 1122 dbSNP
rs976735553 1122 dbSNP
rs1207402585 1124 dbSNP
rs908582006 1126 dbSNP
rs940044791 1133 dbSNP
rs1029267860 1136 dbSNP
rs373804339 1140 dbSNP
rs1346460791 1145 dbSNP
rs898773940 1148 dbSNP
rs1236402005 1151 dbSNP
rs1404423165 1151 dbSNP
rs1480894067 1155 dbSNP
rs932902340 1156 dbSNP
rs1378453949 1160 dbSNP
rs1262464827 1162 dbSNP
rs1194468151 1166 dbSNP
rs1486848997 1166 dbSNP
rs1245065803 1173 dbSNP
rs1221043767 1174 dbSNP
rs1438812918 1178 dbSNP
rs909397769 1180 dbSNP
rs962414199 1182 dbSNP
rs972485679 1184 dbSNP
rs1397103023 1186 dbSNP
rs1279317312 1187 dbSNP
rs764663486 1192 dbSNP
rs918240351 1193 dbSNP
rs1291320065 1195 dbSNP
rs1435274731 1196 dbSNP
rs184800803 1205 dbSNP
rs867787013 1207 dbSNP
rs1333512112 1213 dbSNP
rs1463925812 1222 dbSNP
rs1422656524 1225 dbSNP
rs375201494 1240 dbSNP
rs764607984 1241 dbSNP
rs1444933804 1242 dbSNP
rs1050381326 1243 dbSNP
rs1056766634 1255 dbSNP
rs751163521 1257 dbSNP
rs1296935508 1258 dbSNP
rs1196990900 1259 dbSNP
rs1376865642 1259 dbSNP
rs1008770969 1261 dbSNP
rs1031437315 1262 dbSNP
rs1235480862 1262 dbSNP
rs1310999641 1263 dbSNP
rs1306169186 1268 dbSNP
rs566625299 1275 dbSNP
rs1349531703 1277 dbSNP
rs756892985 1280 dbSNP
rs1277229975 1285 dbSNP
rs1043519275 1287 dbSNP
rs903700900 1287 dbSNP
rs1327417837 1288 dbSNP
rs1444137266 1299 dbSNP
rs1399210565 1300 dbSNP
rs1298563872 1301 dbSNP
rs1437879208 1307 dbSNP
rs891508472 1308 dbSNP
rs767007125 1309 dbSNP
rs1206056139 1313 dbSNP
rs1396982067 1321 dbSNP
rs751966245 1322 dbSNP
rs1239135761 1331 dbSNP
rs1197974070 1332 dbSNP
rs1482553356 1336 dbSNP
rs1021283410 1338 dbSNP
rs1457307359 1349 dbSNP
rs1255989391 1350 dbSNP
rs1211448808 1352 dbSNP
rs576870510 1354 dbSNP
rs1193963819 1371 dbSNP
rs901820252 1374 dbSNP
rs1237459592 1376 dbSNP
rs1375748525 1387 dbSNP
rs997555171 1390 dbSNP
rs1029421384 1394 dbSNP
rs1392979509 1398 dbSNP
rs953585302 1400 dbSNP
rs1477141162 1404 dbSNP
rs544304400 1406 dbSNP
rs1377312571 1407 dbSNP
rs1420554736 1410 dbSNP
rs1439291430 1416 dbSNP
rs1411702082 1420 dbSNP
rs1168512454 1422 dbSNP
rs535034712 1431 dbSNP
rs1442304509 1444 dbSNP
rs1422359065 1447 dbSNP
rs1328268470 1448 dbSNP
rs970004878 1452 dbSNP
rs868244065 1453 dbSNP
rs1486960146 1454 dbSNP
rs1436263896 1458 dbSNP
rs1016408423 1461 dbSNP
rs1273843260 1461 dbSNP
rs1339736066 1462 dbSNP
rs1261018524 1467 dbSNP
rs201386885 1472 dbSNP
rs980009954 1473 dbSNP
rs1317151191 1476 dbSNP
rs1035982475 1480 dbSNP
rs1214278575 1486 dbSNP
rs1274763858 1489 dbSNP
rs1357340042 1491 dbSNP
rs972891402 1491 dbSNP
rs959902313 1493 dbSNP
rs1324237914 1498 dbSNP
rs1385764709 1498 dbSNP
rs796991681 1498 dbSNP
rs1449635202 1499 dbSNP
rs1197954085 1500 dbSNP
rs960641877 1502 dbSNP
rs916253138 1503 dbSNP
rs991811657 1503 dbSNP
rs1190873075 1504 dbSNP
rs1353867627 1508 dbSNP
rs983667229 1508 dbSNP
rs1423017451 1514 dbSNP
rs1233732213 1518 dbSNP
rs1462376473 1518 dbSNP
rs1043656913 1519 dbSNP
rs552910440 1519 dbSNP
rs1327054726 1522 dbSNP
rs1352455381 1523 dbSNP
rs1385280638 1523 dbSNP
rs925113372 1524 dbSNP
rs1405133766 1527 dbSNP
rs1463407393 1528 dbSNP
rs1207536259 1531 dbSNP
rs1326593676 1534 dbSNP
rs1432462436 1535 dbSNP
rs1373119551 1538 dbSNP
rs1275711953 1544 dbSNP
rs1213931099 1545 dbSNP
rs1252061189 1545 dbSNP
rs1449498695 1545 dbSNP
rs1469598598 1545 dbSNP
rs1339304659 1550 dbSNP
rs1206687329 1552 dbSNP
rs1233421853 1552 dbSNP
rs1213884285 1553 dbSNP
rs1231233639 1553 dbSNP
rs1271982217 1553 dbSNP
rs1326752050 1553 dbSNP
rs1330738269 1553 dbSNP
rs878928189 1553 dbSNP
rs1274938083 1555 dbSNP
rs1443398080 1555 dbSNP
rs1354245346 1557 dbSNP
rs908638604 1557 dbSNP
rs1331144724 1558 dbSNP
rs1209010968 1560 dbSNP
rs1410995785 1561 dbSNP
rs1399329446 1562 dbSNP
rs1169137756 1563 dbSNP
rs1284713066 1563 dbSNP
rs11346868 1564 dbSNP
rs1198668071 1564 dbSNP
rs1275118202 1564 dbSNP
rs1304294666 1564 dbSNP
rs1438313518 1564 dbSNP
rs1476715771 1564 dbSNP
rs1488809770 1564 dbSNP
rs199698020 1564 dbSNP
rs201335371 1564 dbSNP
rs70993262 1564 dbSNP
rs75387434 1564 dbSNP
rs1236733264 1565 dbSNP
rs1375016988 1566 dbSNP
rs1448892433 1566 dbSNP
rs1214764750 1568 dbSNP
rs1279322510 1568 dbSNP
rs1356643845 1569 dbSNP
rs1332700514 1570 dbSNP
rs1262797662 1571 dbSNP
rs1173767331 1572 dbSNP
rs1231939090 1572 dbSNP
rs1436176108 1573 dbSNP
rs1475556823 1573 dbSNP
rs1378165618 1574 dbSNP
rs1190231462 1575 dbSNP
rs1234409678 1576 dbSNP
rs1367219122 1576 dbSNP
rs935187114 1576 dbSNP
rs1185269000 1578 dbSNP
rs1255246084 1583 dbSNP
rs1485326561 1583 dbSNP
rs1472765092 1584 dbSNP
rs1159442354 1585 dbSNP
rs1387634883 1586 dbSNP
rs1351775579 1587 dbSNP
rs149981162 1587 dbSNP
rs538745471 1590 dbSNP
rs1421087164 1591 dbSNP
rs1295939658 1594 dbSNP
rs1359550471 1597 dbSNP
rs755830616 1599 dbSNP
rs1052932896 1602 dbSNP
rs1314325967 1604 dbSNP
rs1338573397 1606 dbSNP
rs1312946577 1610 dbSNP
rs1416165035 1611 dbSNP
rs559859461 1613 dbSNP
rs1279883059 1617 dbSNP
rs1157843791 1622 dbSNP
rs901898181 1624 dbSNP
rs1409838806 1627 dbSNP
rs1161294760 1636 dbSNP
rs1258218293 1636 dbSNP
rs1474831234 1636 dbSNP
rs1352605629 1643 dbSNP
rs1487315042 1649 dbSNP
rs1241614741 1656 dbSNP
rs1237738586 1670 dbSNP
rs1285568445 1671 dbSNP
rs998026747 1676 dbSNP
rs557175079 1679 dbSNP
rs200398064 1680 dbSNP
rs1050408770 1682 dbSNP
rs1213040851 1683 dbSNP
rs551616148 1684 dbSNP
rs889127907 1685 dbSNP
rs1287816099 1687 dbSNP
rs556251795 1691 dbSNP
rs749095489 1691 dbSNP
rs770317623 1691 dbSNP
rs1323639493 1692 dbSNP
rs1460665706 1694 dbSNP
rs1418320933 1697 dbSNP
rs575781468 1699 dbSNP
rs1476815925 1701 dbSNP
rs897977974 1701 dbSNP
rs1418850816 1702 dbSNP
rs940088775 1711 dbSNP
rs1486967071 1714 dbSNP
rs974082718 1716 dbSNP
rs1213307615 1717 dbSNP
rs1183189857 1721 dbSNP
rs1366108164 1760 dbSNP
rs1217295986 1774 dbSNP
rs1036285192 1776 dbSNP
rs919919237 1778 dbSNP
rs1158492239 1783 dbSNP
rs189391383 1789 dbSNP
rs1363457575 1797 dbSNP
rs1300730236 1799 dbSNP
rs555267051 1805 dbSNP
rs1394891351 1809 dbSNP
rs932619593 1810 dbSNP
rs749882049 1814 dbSNP
rs868020907 1823 dbSNP
rs1327711250 1825 dbSNP
rs755665020 1826 dbSNP
rs180923473 1828 dbSNP
rs1379291205 1829 dbSNP
rs540233966 1829 dbSNP
rs1434966305 1842 dbSNP
rs1430317428 1843 dbSNP
rs1304014552 1845 dbSNP
rs960466258 1849 dbSNP
rs944232069 1855 dbSNP
rs564867245 1862 dbSNP
rs891577417 1878 dbSNP
rs1197207101 1880 dbSNP
rs200289141 1892 dbSNP
rs969063549 1893 dbSNP
rs1011329842 1906 dbSNP
rs1207259529 1912 dbSNP
rs779963265 1913 dbSNP
rs1308694111 1916 dbSNP
rs905537503 1920 dbSNP
rs1277610089 1931 dbSNP
rs1371339228 1932 dbSNP
rs1001569510 1943 dbSNP
rs925228895 1945 dbSNP
rs1298624908 1947 dbSNP
rs1463484240 1948 dbSNP
rs546828315 1949 dbSNP
rs1173139904 1951 dbSNP
rs1435547522 1954 dbSNP
rs774378839 1955 dbSNP
rs1035630370 1967 dbSNP
rs1179931562 1970 dbSNP
rs184779509 1975 dbSNP
rs1179418010 1979 dbSNP
rs544308760 1982 dbSNP
rs1482584553 1993 dbSNP
rs1277541078 1996 dbSNP
rs868347616 1999 dbSNP
rs1348152912 2000 dbSNP
rs1281942751 2001 dbSNP
rs1236452540 2004 dbSNP
rs1254274898 2005 dbSNP
rs1015168588 2008 dbSNP
rs1344370268 2016 dbSNP
rs1393035269 2021 dbSNP
rs772026753 2023 dbSNP
rs961474714 2031 dbSNP
rs3192372 2036 dbSNP
rs562700047 2048 dbSNP
rs1312345373 2052 dbSNP
rs1415401012 2057 dbSNP
rs1196499982 2058 dbSNP
rs1377368719 2063 dbSNP
rs776616562 2065 dbSNP
rs1253285154 2073 dbSNP
rs1263865380 2076 dbSNP
rs1453354653 2077 dbSNP
rs1364702964 2079 dbSNP
rs1050980175 2082 dbSNP
rs1474503286 2086 dbSNP
rs889119753 2091 dbSNP
rs1189277653 2092 dbSNP
rs974136530 2093 dbSNP
rs942033488 2098 dbSNP
rs1257689653 2100 dbSNP
rs529920193 2102 dbSNP
rs898053220 2103 dbSNP
rs144734333 2110 dbSNP
rs1240993586 2113 dbSNP
rs985834335 2114 dbSNP
rs759689295 2118 dbSNP
rs1233845081 2123 dbSNP
rs1035825909 2131 dbSNP
rs1375629792 2145 dbSNP
rs1316358636 2151 dbSNP
rs1381839130 2156 dbSNP
rs1386018973 2166 dbSNP
rs1290999639 2168 dbSNP
rs1465101277 2170 dbSNP
rs367694993 2172 dbSNP
rs1166228169 2174 dbSNP
rs895982070 2174 dbSNP
rs1168047373 2192 dbSNP
rs1369662811 2195 dbSNP
rs566769836 2195 dbSNP
rs527546877 2199 dbSNP
rs944270672 2200 dbSNP
rs1190461919 2202 dbSNP
rs1023362818 2206 dbSNP
rs1261911607 2206 dbSNP
rs1446053588 2206 dbSNP
rs1466931749 2207 dbSNP
rs969010916 2210 dbSNP
rs1214209127 2215 dbSNP
rs1053331039 2216 dbSNP
rs1268476654 2217 dbSNP
rs1226871084 2221 dbSNP
rs765617011 2235 dbSNP
rs879249672 2235 dbSNP
rs1439017987 2236 dbSNP
rs778685534 2236 dbSNP
rs1394158384 2239 dbSNP
rs1327042878 2241 dbSNP
rs1463455540 2242 dbSNP
rs913397592 2250 dbSNP
rs747212000 2254 dbSNP
rs1042831160 2260 dbSNP
rs1299428421 2262 dbSNP
rs905588228 2263 dbSNP
rs1452227689 2267 dbSNP
rs1491229148 2268 dbSNP
rs1001222943 2269 dbSNP
rs1491122788 2269 dbSNP
rs546635793 2270 dbSNP
rs1260092401 2275 dbSNP
rs571298531 2294 dbSNP
rs1276905495 2299 dbSNP
rs988470093 2306 dbSNP
rs923120469 2314 dbSNP
rs933189299 2323 dbSNP
rs189589692 2325 dbSNP
rs1057190113 2332 dbSNP
rs986197173 2336 dbSNP
rs1411044860 2341 dbSNP
rs910668769 2341 dbSNP
rs1470092400 2347 dbSNP
rs1432460895 2348 dbSNP
rs942106961 2352 dbSNP
rs895770773 2359 dbSNP
rs1193880754 2361 dbSNP
rs1189904825 2362 dbSNP
rs1230986934 2364 dbSNP
rs1264805709 2364 dbSNP
rs775784146 2365 dbSNP
rs1180956230 2384 dbSNP
rs1037683545 2389 dbSNP
rs550640811 2390 dbSNP
rs1208895108 2396 dbSNP
rs1347425361 2400 dbSNP
rs1281225984 2401 dbSNP
rs1221175671 2403 dbSNP
rs1005199306 2407 dbSNP
rs1463953369 2409 dbSNP
rs569227343 2423 dbSNP
rs760294006 2424 dbSNP
rs766072914 2429 dbSNP
rs1443533130 2434 dbSNP
rs771079525 2434 dbSNP
rs759035843 2437 dbSNP
rs544262784 2452 dbSNP
rs895964575 2452 dbSNP
rs994604125 2452 dbSNP
rs1157832315 2456 dbSNP
rs1044553032 2468 dbSNP
rs904926250 2469 dbSNP
rs1459246189 2470 dbSNP
rs1000573405 2473 dbSNP
rs1258354571 2475 dbSNP
rs762176006 2476 dbSNP
rs552581661 2480 dbSNP
rs1009484580 2482 dbSNP
rs1224749203 2484 dbSNP
rs985500275 2485 dbSNP
rs776959407 2500 dbSNP
rs1233237648 2502 dbSNP
rs745904663 2512 dbSNP
rs954555766 2514 dbSNP
rs1052050 2516 dbSNP
rs770060901 2517 dbSNP
rs1280132699 2528 dbSNP
rs1343131325 2530 dbSNP
rs764809326 2530 dbSNP
rs1218149956 2532 dbSNP
rs11556729 2535 dbSNP
rs910408110 2535 dbSNP
rs989478143 2536 dbSNP
rs1162649838 2540 dbSNP
rs1474862168 2541 dbSNP
rs1324083572 2542 dbSNP
rs973493435 2542 dbSNP
rs1474544630 2543 dbSNP
rs1219744026 2544 dbSNP
rs1216785533 2551 dbSNP
rs1265227940 2552 dbSNP
rs1487930938 2555 dbSNP
rs1216910014 2564 dbSNP
rs1271699979 2567 dbSNP
rs1213584357 2568 dbSNP
rs919258014 2571 dbSNP
rs1264945538 2572 dbSNP
rs1312791240 2578 dbSNP
rs1229602606 2579 dbSNP
rs1359262670 2580 dbSNP
rs929319327 2582 dbSNP
rs913449631 2590 dbSNP
rs1192387395 2595 dbSNP
rs947554617 2595 dbSNP
rs1418468691 2599 dbSNP
rs1390523891 2604 dbSNP
rs1057367369 2617 dbSNP
rs1460846565 2617 dbSNP
rs376065005 2617 dbSNP
rs776123291 2621 dbSNP
rs763565285 2636 dbSNP
rs1166833745 2651 dbSNP
rs917458855 2653 dbSNP
rs1418920083 2661 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545216. RNA binding protein: AGO2. Condition:miR-124 transfection ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084079. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' accuucugaucacUAAAACAACAa 5'
                       || ||||||| 
Target 5' -------------AUGUUGUUGUu 3'
 		1 - 11
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084079
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_hippuristanol_rep2_AbnovaAb
Location of target site ENST00000244745.1 | 3UTR | AUGUUGUUGUUGAUGGCAAAAAAAAAAAAGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM545216
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / miR-124 transfection
Location of target site ENST00000244745.1 | 3UTR | UUACCCACGCCAUUUUACGUCUCCUUCACUGAAGGGCUAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
101 hsa-miR-3529-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT061395 WEE1 WEE1 G2 checkpoint kinase 2 2
MIRT066530 HMGA2 high mobility group AT-hook 2 2 6
MIRT072699 SMAD3 SMAD family member 3 2 2
MIRT080963 LRRC8B leucine rich repeat containing 8 VRAC subunit B 2 2
MIRT095081 SAR1B secretion associated Ras related GTPase 1B 2 2
MIRT104782 FZD6 frizzled class receptor 6 2 2
MIRT161286 U2SURP U2 snRNP associated SURP domain containing 2 2
MIRT176138 ATP7A ATPase copper transporting alpha 2 2
MIRT221376 DNAJB6 DnaJ heat shock protein family (Hsp40) member B6 2 2
MIRT260611 SOX4 SRY-box 4 2 4
MIRT269640 MDM4 MDM4, p53 regulator 2 4
MIRT306853 FYTTD1 forty-two-three domain containing 1 2 2
MIRT315027 TFAP2A transcription factor AP-2 alpha 2 2
MIRT367278 CRKL CRK like proto-oncogene, adaptor protein 2 2
MIRT444211 TMPRSS4 transmembrane protease, serine 4 2 2
MIRT455829 ZSWIM1 zinc finger SWIM-type containing 1 2 2
MIRT466299 TM4SF1 transmembrane 4 L six family member 1 2 2
MIRT467332 SPATA13 spermatogenesis associated 13 2 4
MIRT469830 RAB14 RAB14, member RAS oncogene family 2 2
MIRT470571 POU2F1 POU class 2 homeobox 1 2 2
MIRT472094 NOLC1 nucleolar and coiled-body phosphoprotein 1 2 2
MIRT482529 ACTB actin beta 2 4
MIRT495009 KRTAP4-9 keratin associated protein 4-9 2 4
MIRT495562 ZNF169 zinc finger protein 169 2 2
MIRT496742 PBX2P1 PBX homeobox 2 pseudogene 1 2 4
MIRT500811 THBS1 thrombospondin 1 2 2
MIRT500846 SYPL1 synaptophysin like 1 2 4
MIRT504267 C1orf147 chromosome 1 open reading frame 147 2 4
MIRT504320 TUSC1 tumor suppressor candidate 1 2 6
MIRT505533 SPATA2 spermatogenesis associated 2 2 4
MIRT505799 RSBN1 round spermatid basic protein 1 2 4
MIRT518201 CLEC4E C-type lectin domain family 4 member E 2 2
MIRT520019 YTHDF1 YTH N6-methyladenosine RNA binding protein 1 2 4
MIRT527321 NCAM2 neural cell adhesion molecule 2 2 2
MIRT529005 SIGLEC14 sialic acid binding Ig like lectin 14 2 4
MIRT530312 TNFRSF10D TNF receptor superfamily member 10d 2 2
MIRT532324 DUSP4 dual specificity phosphatase 4 2 2
MIRT532497 HOXA13 homeobox A13 2 2
MIRT533203 WASF3 WAS protein family member 3 2 4
MIRT533376 UBE2D4 ubiquitin conjugating enzyme E2 D4 (putative) 2 4
MIRT534107 SOWAHC sosondowah ankyrin repeat domain family member C 2 2
MIRT537381 FGF2 fibroblast growth factor 2 2 2
MIRT537487 FAM169A family with sequence similarity 169 member A 2 2
MIRT539210 ANTXR2 anthrax toxin receptor 2 2 4
MIRT540299 GFPT1 glutamine--fructose-6-phosphate transaminase 1 2 2
MIRT544610 FCF1 FCF1, rRNA-processing protein 2 2
MIRT547382 MPRIP myosin phosphatase Rho interacting protein 2 2
MIRT551195 GAB2 GRB2 associated binding protein 2 2 2
MIRT554193 SLC35D1 solute carrier family 35 member D1 2 2
MIRT554998 RAB39B RAB39B, member RAS oncogene family 2 2
MIRT557252 CRAMP1L cramped chromatin regulator homolog 1 2 2
MIRT558440 DDIT4 DNA damage inducible transcript 4 2 3
MIRT558687 CMIP c-Maf inducing protein 2 2
MIRT562111 ITGB1 integrin subunit beta 1 2 2
MIRT562312 GATAD2A GATA zinc finger domain containing 2A 2 2
MIRT564781 ZDHHC7 zinc finger DHHC-type containing 7 2 2
MIRT565536 SON SON DNA binding protein 2 2
MIRT567294 HNRNPA2B1 heterogeneous nuclear ribonucleoprotein A2/B1 2 2
MIRT572337 SOX5 SRY-box 5 2 2
MIRT572389 MTX3 metaxin 3 2 2
MIRT575082 Ddit4 DNA-damage-inducible transcript 4 2 3
MIRT576838 Tgfbr3 transforming growth factor, beta receptor III 2 2
MIRT607441 SEMA6D semaphorin 6D 2 4
MIRT609555 TCEAL4 transcription elongation factor A like 4 2 2
MIRT612890 HOOK3 hook microtubule tethering protein 3 2 2
MIRT613611 TP53 tumor protein p53 2 2
MIRT614708 TNFRSF21 TNF receptor superfamily member 21 2 2
MIRT616823 HERC5 HECT and RLD domain containing E3 ubiquitin protein ligase 5 2 2
MIRT620652 CXCL5 C-X-C motif chemokine ligand 5 2 2
MIRT626770 CDC14B cell division cycle 14B 2 2
MIRT633598 IBA57 IBA57 homolog, iron-sulfur cluster assembly 2 2
MIRT634853 PTP4A2 protein tyrosine phosphatase type IVA, member 2 2 2
MIRT638138 TTC26 tetratricopeptide repeat domain 26 2 2
MIRT639574 GORASP1 golgi reassembly stacking protein 1 2 2
MIRT643710 ZNF736 zinc finger protein 736 2 2
MIRT644969 STEAP4 STEAP4 metalloreductase 2 2
MIRT645427 NINJ1 ninjurin 1 2 2
MIRT645939 HIPK1 homeodomain interacting protein kinase 1 2 2
MIRT646142 INTS7 integrator complex subunit 7 2 2
MIRT658015 GABRA4 gamma-aminobutyric acid type A receptor alpha4 subunit 2 2
MIRT658995 DIP2C disco interacting protein 2 homolog C 2 2
MIRT675003 SLC22A17 solute carrier family 22 member 17 2 2
MIRT675553 SIK1 salt inducible kinase 1 2 2
MIRT675621 CSE1L chromosome segregation 1 like 2 2
MIRT675952 IDE insulin degrading enzyme 2 2
MIRT692250 POLR3F RNA polymerase III subunit F 2 2
MIRT698738 STX12 syntaxin 12 2 2
MIRT699001 SPAG9 sperm associated antigen 9 2 2
MIRT703275 GNG12 G protein subunit gamma 12 2 2
MIRT706268 MKLN1 muskelin 1 2 2
MIRT707633 TARDBP TAR DNA binding protein 2 2
MIRT709622 KBTBD6 kelch repeat and BTB domain containing 6 2 2
MIRT712931 RPF2 ribosome production factor 2 homolog 2 2
MIRT713807 KCNK5 potassium two pore domain channel subfamily K member 5 2 2
MIRT714036 SYDE2 synapse defective Rho GTPase homolog 2 2 2
MIRT714388 PDP2 pyruvate dehyrogenase phosphatase catalytic subunit 2 2 2
MIRT714404 FBXO31 F-box protein 31 2 2
MIRT714599 HSPA4L heat shock protein family A (Hsp70) member 4 like 2 2
MIRT714663 TPCN2 two pore segment channel 2 2 2
MIRT714882 GOLPH3 golgi phosphoprotein 3 2 2
MIRT725043 NDUFAF7 NADH:ubiquinone oxidoreductase complex assembly factor 7 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-3529-3p Erlotinib 176870 NSC718781 approved sensitive High Head And Neck Squamous Cell Carcinoma cell line (HN6)
hsa-miR-3529-3p Paclitaxel 36314 NSC125973 approved sensitive cell line (A2780)

Error report submission