miRTarBase - #MIRT277106


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pre-miRNA Information
pre-miRNA hsa-mir-371b   
Genomic Coordinates chr19: 53787677 - 53787742
Description Homo sapiens miR-371b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-371b-3p
Sequence 43| AAGUGCCCCCACAGUUUGAGUGC |65
Evidence Experimental
Experiments Illumina
Editing Events in miRNAs
Modification Type Position on miR Chromosome DNA Strand Genomic Position (hg38) List of PMIDs Variant details
A-to-I 2 19 - 53787699 16594986 MiREDiBase
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1345825760 2 dbSNP
rs922443786 5 dbSNP
rs773769548 6 dbSNP
rs1034874198 8 dbSNP
rs772543475 9 dbSNP
rs1318684148 10 dbSNP
rs1004685112 12 dbSNP
rs1402402708 12 dbSNP
rs1427092890 13 dbSNP
rs1202234248 14 dbSNP
rs770734168 14 dbSNP
rs1428290628 15 dbSNP
rs1480794990 15 dbSNP
rs1049004182 17 dbSNP
rs1185000692 19 dbSNP
rs748499899 22 dbSNP
rs1487511363 23 dbSNP
Putative Targets

Gene Information
Gene Symbol CDCA8   
Synonyms BOR, BOREALIN, DasraB, MESRGP
Description cell division cycle associated 8
Transcript NM_018101   
Expression
Putative miRNA Targets on CDCA8
3'UTR of CDCA8
(miRNA target sites are highlighted)		 
>CDCA8|NM_018101|3'UTR
   1 GACACCAAAGTTGACAGGATGGACTTTTAATGGGCACTTCTGGGACCCTGAAGAGACTTCTTCCCTTCAGGCTTATTGTT
  81 TGAGTGTGAAGTTCCAGAGCAAGGAGCCATGTTCCTCTAAGGGAATTCAGGAATTCAGACGTGCTAGTCCCACACCAGTT
 161 AGGTAGAGCTGTCTGTTCACCCTCCCATCCCAGCTGATCCCAGTCACTGCTTGCTGGGGCCATGCCATGGAAGCTTCCCA
 241 TCAGTCTCCCAGCTGAATCCTCCCTGCTCTCTGAGCTGCTGCCTTTTGCCTCCTGCAACTCAACATCCTCTTCACCCTGC
 321 CCTGCCTGCAGTTGAGGGGGCGAAGAAGAACCCTGTGTTCTCAGGAAGACTGCCTCCACCACCGCTACCCAGAGAACCTC
 401 TGCATCTGGCATTTCTGCTCTCTATGCTTGAGACCGGGAGGTTTAGGCTCAGATAAGTGAGCTCTGGGCCATGAGAGGGT
 481 AGGTCCAGAAGGTGGGGGGAACTGTACAGATCAGCAGAGCAGGACAGTTGGCAGCAGTGACCTCAGTAGGGAACATGTCC
 561 GTCTACCCTCTCGCACTCATGACACCTCCCCCTACCAGCCCTCCTCTTCCTCCTCCTCCTCCTCCTGTGGGAGGTGGTCA
 641 GTGGGACTTAGGGATCTTTCACCTGCTGTGCCCAGTAGTTCTGAAGTCTGCTTGTGGAGCAGTGTTTTATGTTTATCCCT
 721 GTTTACTGAAGACCAAATACTGGTTTGGAGACAACTTCCATGTCTTGCTCTTCTACCTCCCTAGTTAGTGGAAATTTGGA
 801 TAAGGGAACTGTAGGGCCCAGATTCTGGAGGTTTTATGTCATTGGCCACAGAATAACTGTCTCTAAGCTATCCATGGTCC
 881 AGTGGTCCCTGCCAAGTCTGTAGACTTCAGAGAGCACTTCTCTCTTATGGGGTTCATGGGAACAGGGGTGGGTGTGACTT
 961 GCTTGGTGGCCTCATTCCATGTGTGCCTGTGCCTGGGGCATGGACTTTGTTAAGCAGAGTCAGCAGTGAGGTCCTCATTC
1041 TCCAGCCAGCCTCTCTGCCCTGGAGAATCATGTGCTATGTTCTAAGAATTTGAGAACTAGAGTCCTCATCCCCAGGCTTG
1121 AAGGCACATGGCTTTCTCATGTAGGGCTCTCTGTGGTATTTGTTATTATTTTGCAACAAGACCATTTTAGTAAAACAGTC
1201 CTGTTCAAGTTGTATTCTTTTAAGTTCTTTTATTCTCCTTTCCCTGAGATTTTTGTATATATTGTTCTGAGTAATGGTAT
1281 CTTTGAGCTGATTGTTCTAATCAGAGCTGGTACCTACTTTCAATAAATTCTGGTTTTGTGTTTTCTTTTGTAAAAAAAAA
1361 AAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cgugaGUUUGACA--CCCCCGUGAa 5'
               :::||| |    ||||||| 
Target 5' caggaTGGACTTTTAATGGGCACTt 3'
 15 - 39 144.00 -13.10
2 
miRNA  3' cgugaGUUUGACA-CCCCCGUGAa 5'
               ||:|  || ||||| ||| 
Target 5' aggtcCAGAAGGTGGGGGGAACTg 3'
 481 - 504 130.00 -20.30
3 
miRNA  3' cgUGAGUUUGACACC----CCCGUGAa 5'
            | | :|| | |||    ||| ||| 
Target 5' ttAGTGGAAATTTGGATAAGGGAACTg 3'
 785 - 811 123.00 -8.00
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30480136 64 COSMIC
COSN30100412 65 COSMIC
COSN31500762 79 COSMIC
COSN30103867 128 COSMIC
COSN6455280 148 COSMIC
COSN30533661 209 COSMIC
COSN8468595 303 COSMIC
COSN6455281 338 COSMIC
COSN22267417 568 COSMIC
COSN22820263 609 COSMIC
COSN9025869 969 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs773393864 1 dbSNP
rs963695643 2 dbSNP
rs763562026 5 dbSNP
rs1473882957 6 dbSNP
rs1182368635 8 dbSNP
rs771294084 11 dbSNP
rs371231677 16 dbSNP
rs368820074 17 dbSNP
rs1363048417 19 dbSNP
rs1451327439 21 dbSNP
rs1479881281 22 dbSNP
rs933868871 23 dbSNP
rs1406721591 33 dbSNP
rs1312441009 36 dbSNP
rs1215193200 37 dbSNP
rs1046984213 40 dbSNP
rs372414342 47 dbSNP
rs1211251108 50 dbSNP
rs941235419 51 dbSNP
rs752414866 52 dbSNP
rs1402773810 53 dbSNP
rs1207742857 57 dbSNP
rs916344223 65 dbSNP
rs1223074508 66 dbSNP
rs762061971 72 dbSNP
rs894585453 73 dbSNP
rs1276377745 75 dbSNP
rs1432835700 76 dbSNP
rs1468561684 79 dbSNP
rs1442006098 80 dbSNP
rs947832729 85 dbSNP
rs1046229615 86 dbSNP
rs1345822766 92 dbSNP
rs1159023710 98 dbSNP
rs1236300790 105 dbSNP
rs752395591 107 dbSNP
rs906395276 111 dbSNP
rs1194619626 121 dbSNP
rs1439069983 122 dbSNP
rs528212130 135 dbSNP
rs1052806724 141 dbSNP
rs1270658043 142 dbSNP
rs1365360537 147 dbSNP
rs557897 148 dbSNP
rs1486561838 155 dbSNP
rs1011547286 164 dbSNP
rs1393974542 165 dbSNP
rs769962057 174 dbSNP
rs1462039577 176 dbSNP
rs1215052902 179 dbSNP
rs900597296 183 dbSNP
rs41308252 184 dbSNP
rs1398190403 197 dbSNP
rs996256071 203 dbSNP
rs1446784422 206 dbSNP
rs1030490781 220 dbSNP
rs1308301643 225 dbSNP
rs1337955568 227 dbSNP
rs1234721983 240 dbSNP
rs1243007675 250 dbSNP
rs955073839 253 dbSNP
rs371044448 262 dbSNP
rs1437468903 263 dbSNP
rs374096959 264 dbSNP
rs555004161 268 dbSNP
rs1288352694 275 dbSNP
rs1452306832 281 dbSNP
rs1361380826 288 dbSNP
rs999473097 295 dbSNP
rs192535651 299 dbSNP
rs1393584799 304 dbSNP
rs567088897 306 dbSNP
rs1456905102 309 dbSNP
rs1429911815 310 dbSNP
rs1261899972 314 dbSNP
rs7526362 329 dbSNP
rs866148595 332 dbSNP
rs559707 338 dbSNP
rs41303419 342 dbSNP
rs975174414 342 dbSNP
rs72659465 343 dbSNP
rs935181282 346 dbSNP
rs1417434241 355 dbSNP
rs1052334581 357 dbSNP
rs1167366771 358 dbSNP
rs1295004465 369 dbSNP
rs148036658 378 dbSNP
rs963260713 378 dbSNP
rs1461526337 395 dbSNP
rs1381371799 399 dbSNP
rs1314021991 405 dbSNP
rs947350881 411 dbSNP
rs542000997 418 dbSNP
rs1043036338 422 dbSNP
rs1299264628 423 dbSNP
rs900544493 425 dbSNP
rs996261609 435 dbSNP
rs1373269410 436 dbSNP
rs1399178932 437 dbSNP
rs1038180816 439 dbSNP
rs1394666734 448 dbSNP
rs915757235 459 dbSNP
rs561306039 461 dbSNP
rs376047393 475 dbSNP
rs1356500158 479 dbSNP
rs1234788651 483 dbSNP
rs1275885835 492 dbSNP
rs1225428522 498 dbSNP
rs1045796486 503 dbSNP
rs1316771386 506 dbSNP
rs1005472721 508 dbSNP
rs1233282457 512 dbSNP
rs1015140561 521 dbSNP
rs1253016021 523 dbSNP
rs530845728 524 dbSNP
rs561628438 533 dbSNP
rs963622884 535 dbSNP
rs141712543 546 dbSNP
rs1297255910 551 dbSNP
rs1460104574 558 dbSNP
rs1486512883 561 dbSNP
rs527297834 562 dbSNP
rs969068910 573 dbSNP
rs765167727 574 dbSNP
rs528072481 580 dbSNP
rs1171155213 584 dbSNP
rs150595350 586 dbSNP
rs1419455116 588 dbSNP
rs1249481392 589 dbSNP
rs927716952 591 dbSNP
rs996561166 600 dbSNP
rs1015692209 608 dbSNP
rs547685290 608 dbSNP
rs955198409 608 dbSNP
rs982490624 608 dbSNP
rs1240306478 611 dbSNP
rs564350121 616 dbSNP
rs1305297989 622 dbSNP
rs988382196 623 dbSNP
rs1465040311 625 dbSNP
rs3748680 628 dbSNP
rs1326104138 631 dbSNP
rs1460491315 635 dbSNP
rs915705000 637 dbSNP
rs948594858 638 dbSNP
rs117536194 642 dbSNP
rs1455783686 654 dbSNP
rs1039624247 675 dbSNP
rs185368132 677 dbSNP
rs1467199268 696 dbSNP
rs1297175589 704 dbSNP
rs1309933872 706 dbSNP
rs1233529189 707 dbSNP
rs1350590558 709 dbSNP
rs921917883 712 dbSNP
rs189849357 717 dbSNP
rs1349815078 726 dbSNP
rs1051826915 747 dbSNP
rs1053103134 748 dbSNP
rs61150145 750 dbSNP
rs1261320015 753 dbSNP
rs1303694344 762 dbSNP
rs1485697675 764 dbSNP
rs765093464 768 dbSNP
rs1328905631 775 dbSNP
rs1187324053 778 dbSNP
rs1401259060 780 dbSNP
rs1263993117 782 dbSNP
rs1156712386 784 dbSNP
rs181762040 785 dbSNP
rs1039308053 789 dbSNP
rs1173262745 792 dbSNP
rs1452796372 796 dbSNP
rs1419662107 797 dbSNP
rs570545062 799 dbSNP
rs1036527637 811 dbSNP
rs1193959549 815 dbSNP
rs1446985801 837 dbSNP
rs1260297495 845 dbSNP
rs1396164691 851 dbSNP
rs1213558625 854 dbSNP
rs1349059079 855 dbSNP
rs899362489 857 dbSNP
rs997711694 863 dbSNP
rs1029397797 875 dbSNP
rs1271478125 883 dbSNP
rs1430851170 894 dbSNP
rs750359887 901 dbSNP
rs549936422 907 dbSNP
rs1296274614 912 dbSNP
rs1416481546 914 dbSNP
rs534687648 917 dbSNP
rs1344965031 919 dbSNP
rs185028210 929 dbSNP
rs574876896 930 dbSNP
rs1454055650 944 dbSNP
rs969053991 944 dbSNP
rs1421891613 945 dbSNP
rs7822 950 dbSNP
rs1034736646 951 dbSNP
rs1004380710 953 dbSNP
rs1190765645 963 dbSNP
rs557137535 970 dbSNP
rs74737759 976 dbSNP
rs1439459607 986 dbSNP
rs1209148439 993 dbSNP
rs1439868691 996 dbSNP
rs1274713636 999 dbSNP
rs1214725955 1003 dbSNP
rs1304107958 1004 dbSNP
rs956658381 1005 dbSNP
rs1015225455 1009 dbSNP
rs987942142 1028 dbSNP
rs1312107655 1044 dbSNP
rs575320310 1047 dbSNP
rs973958309 1051 dbSNP
rs546147763 1057 dbSNP
rs1355990569 1059 dbSNP
rs1237852162 1070 dbSNP
rs915063414 1080 dbSNP
rs967878121 1085 dbSNP
rs540591737 1095 dbSNP
rs1317257757 1110 dbSNP
rs969851716 1118 dbSNP
rs746507188 1121 dbSNP
rs1475848338 1123 dbSNP
rs981265330 1139 dbSNP
rs564358134 1140 dbSNP
rs928478222 1141 dbSNP
rs934734415 1142 dbSNP
rs1233754259 1147 dbSNP
rs1487563278 1147 dbSNP
rs989311363 1150 dbSNP
rs756752728 1154 dbSNP
rs780447430 1155 dbSNP
rs572917644 1159 dbSNP
rs770821503 1160 dbSNP
rs1456013711 1163 dbSNP
rs868091658 1164 dbSNP
rs749850622 1169 dbSNP
rs921193004 1169 dbSNP
rs143468229 1170 dbSNP
rs1334870449 1176 dbSNP
rs1292633718 1179 dbSNP
rs867350962 1206 dbSNP
rs189458499 1209 dbSNP
rs1375327972 1213 dbSNP
rs933576591 1214 dbSNP
rs1420028008 1222 dbSNP
rs1441800838 1243 dbSNP
rs1051903311 1246 dbSNP
rs528999379 1259 dbSNP
rs766138111 1261 dbSNP
rs1393740773 1268 dbSNP
rs940760972 1268 dbSNP
rs1347714722 1272 dbSNP
rs1036474641 1273 dbSNP
rs1176955975 1286 dbSNP
rs397831748 1288 dbSNP
rs1460471192 1289 dbSNP
rs1326063684 1290 dbSNP
rs1047958 1298 dbSNP
rs1444521737 1299 dbSNP
rs1037227992 1304 dbSNP
rs1278859457 1311 dbSNP
rs1195072559 1312 dbSNP
rs1468545292 1320 dbSNP
rs898152388 1322 dbSNP
rs930812948 1324 dbSNP
rs1373809588 1328 dbSNP
rs1225216965 1338 dbSNP
rs1022819160 1339 dbSNP
rs969925297 1340 dbSNP
rs1223022775 1343 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545214. RNA binding protein: AGO3. Condition:Control PAR-CLIP data was present in GSM545216. RNA binding protein: AGO2. Condition:miR-124 transfection ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' cgUGAGUUUGACACCCCCGUGAa 5'
            |||:  | |    ||||||| 
Target 5' --ACUUUUAAU----GGGCACUu 3'
 		1 - 17
Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
CLIP-seq Support 1 for dataset GSM545214
Method / RBP PAR-CLIP / AGO3
Cell line / Condition HEK293 / Control
Location of target site ENST00000373055.1 | 3UTR | ACUUUUAAUGGGCACUUCUGGGACCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM545216
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / miR-124 transfection
Location of target site ENST00000373055.1 | 3UTR | ACUUUUAAUGGGCACUUCUGGGACCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
42 hsa-miR-371b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT055410 SHOC2 SHOC2, leucine rich repeat scaffold protein 2 6
MIRT065883 GDF11 growth differentiation factor 11 2 2
MIRT183507 BTG2 BTG anti-proliferation factor 2 2 2
MIRT277106 CDCA8 cell division cycle associated 8 2 2
MIRT300592 CRKL CRK like proto-oncogene, adaptor protein 2 2
MIRT441361 ZNF75A zinc finger protein 75a 2 2
MIRT442692 COX15 COX15, cytochrome c oxidase assembly homolog 2 2
MIRT443388 CHML CHM like, Rab escort protein 2 2 2
MIRT443571 EVX2 even-skipped homeobox 2 2 2
MIRT443671 FLT1 fms related tyrosine kinase 1 2 2
MIRT465524 PRICKLE4 prickle planar cell polarity protein 4 2 2
MIRT492169 STAT3 signal transducer and activator of transcription 3 2 2
MIRT496992 TMEM231 transmembrane protein 231 2 2
MIRT509012 FBXO6 F-box protein 6 2 2
MIRT509766 NCAPD2 non-SMC condensin I complex subunit D2 2 2
MIRT514033 BNIP2 BCL2 interacting protein 2 2 2
MIRT524995 AFF1 AF4/FMR2 family member 1 2 10
MIRT529899 C1orf64 steroid receptor associated and regulated protein 2 2
MIRT530045 SMC1A structural maintenance of chromosomes 1A 2 2
MIRT534479 SAR1B secretion associated Ras related GTPase 1B 2 2
MIRT535065 PPP2R5D protein phosphatase 2 regulatory subunit B'delta 2 4
MIRT539151 AREL1 apoptosis resistant E3 ubiquitin protein ligase 1 2 2
MIRT540937 OIP5 Opa interacting protein 5 2 2
MIRT546360 SYNM synemin 2 2
MIRT553436 TPM3 tropomyosin 3 2 2
MIRT556278 MAPK1 mitogen-activated protein kinase 1 2 2
MIRT564092 NSA2 NSA2, ribosome biogenesis homolog 2 2
MIRT568376 ATXN1 ataxin 1 2 2
MIRT572255 ANKRD52 ankyrin repeat domain 52 2 2
MIRT572453 TRIM10 tripartite motif containing 10 2 2
MIRT610741 NUDT16 nudix hydrolase 16 2 4
MIRT622754 PHACTR2 phosphatase and actin regulator 2 2 2
MIRT627220 ZC3H12B zinc finger CCCH-type containing 12B 2 2
MIRT629555 SPN sialophorin 2 2
MIRT640288 MAP3K9 mitogen-activated protein kinase kinase kinase 9 2 2
MIRT651922 UEVLD UEV and lactate/malate dehyrogenase domains 2 2
MIRT684290 CDK9 cyclin dependent kinase 9 2 2
MIRT700230 REL REL proto-oncogene, NF-kB subunit 2 2
MIRT702748 IGFBP3 insulin like growth factor binding protein 3 2 2
MIRT711110 TBC1D21 TBC1 domain family member 21 2 2
MIRT716402 SEPT5 septin 5 2 2
MIRT723677 CTC1 CST telomere replication complex component 1 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-371b Gemcitabine 60750 NSC613327 approved resistant High Pancreatic Cancer cell line (SW1990)
hsa-miR-371b-3p Gemcitabine 60750 NSC613327 approved sensitive High Pancreatic Cancer cell line (SW1990)
hsa-miR-371b-3p Osimertinib 71496458 NSC779217 approved resistant cell line (PC9)
hsa-miR-371b-3p Vemurafenib 42611257 NSC761431 approved resistant cell line (LM36)

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