miRTarBase - #MIRT286013


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pre-miRNA Information
pre-miRNA hsa-mir-4323   
Genomic Coordinates chr19: 42133445 - 42133513
Description Homo sapiens miR-4323 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4323
Sequence 42| CAGCCCCACAGCCUCAGA |59
Evidence Experimental
Experiments SOLiD
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs549311114 1 dbSNP
rs896027609 6 dbSNP
rs1057502968 11 dbSNP
rs773276005 11 dbSNP
rs1454618841 12 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol TVP23B   
Synonyms CGI-148, FAM18B, FAM18B1, NPD008, YDR084C
Description trans-golgi network vesicle protein 23 homolog B
Transcript NM_016078   
Expression
Putative miRNA Targets on TVP23B
3'UTR of TVP23B
(miRNA target sites are highlighted)		 
>TVP23B|NM_016078|3'UTR
   1 ATAGAGAAAGCTTATGTGCTTTGTTACATTGGGGAACAACTGAAGAGATTCTTGACTCAACCTTTTAGAGCTTAGTCCAT
  81 GTTGCAACGAGGAGTGTTGGCTTTGTTTTTCCACTTAAAAACTTTATTTATAAAAAGGAAAAGTAGTTTTCATATTAAGT
 161 TTTTATTTCCTTTCCAGCAGTTGGGGCTAGAAAGTATGTGTTGGCACTAGAAACATTGTCAAGATTTGTTCTGTGGTGTA
 241 GGTATGCACATTCCATAGGTATGCACACGGCCATGTAATATCAGTATATCCCAAGTTAATGAAAGTGTTCATTTACATAG
 321 GTAATGGAGACCTTTGCATTTTGATCCATAGAACATAGGAGGATGTTCTTAGTCTGTCTCAAAGCTCTATATGTTTACAT
 401 ATTATTTCTGTAGATTGTTTTCAGGAGAAAGTTTTGCTTCTATGGTAAGAGTGAGCACTTTGGCTTATGTATAAGTTAGA
 481 AATAATTGTTAGTTTTTAATATGCACTTCGTGGGGAAATTTCTTAGACGTATGCAAGCAAGTGAAAACAATTAGGGCCAG
 561 TGGTATTAACTACTTTATAAAATTTTATTTTTGTTTGTAAGAAGTCATCTATTTAAGGCCCAGTTAATATAAGTGGAATC
 641 ATCATAGTTTAAGGAATACCCAGAGATTGCTGCTGTTCTATTTATTTTACAGAAAGGATAGCTAGATTGAAAGCTCTTCA
 721 GTGGACCTTGAGCTAATAGATCTTTTACCACTAAAAGAGCATTATTCTCATGTCATAATGAGAATAATAATTTACATACT
 801 TGGCATAATAAATGCCTAAAAGACATTTTATTTTCTGAATCTATTTTTTTCTTGCTATAATGGGGATATTGTAAATTATG
 881 CATTTGTATTAATGGTATTTCTTAAAGCAATCTATGTAACTGTAAATTAAACCAATCTACAAACTATTGTAGGCATCTGT
 961 AAATTCTGTTGTAGGTATTATAAACTTTGTTGAAGTCTTAATCAGCAGATTATGTTGTGAATATATTTGTACATTGTTAA
1041 AATAGTTTTTAAGATTATTTGTTTAATTGAATAAGTGTCTTATTGGAGTGATAGCTTTGAAGGTGCAAAACTTTATATTT
1121 GTATAAAATTCTACTATTTACAAGGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agacucCGAC-ACCCCGAc 5'
                || | ||||||| 
Target 5' tttccaGCAGTTGGGGCTa 3'
 171 - 189 144.00 -11.90
2 
miRNA  3' agacuccgacACCCCGAc 5'
                    ||||| | 
Target 5' cttgctataaTGGGGATa 3'
 851 - 868 108.00 -8.52
3 
miRNA  3' agacuccgaCACCCCgac 5'
                   ||||||   
Target 5' atgcacttcGTGGGGaaa 3'
 501 - 518 105.00 -10.72
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN8407546 5 COSMIC
COSN30100944 13 COSMIC
COSN30115372 27 COSMIC
COSN30482244 52 COSMIC
COSN30522317 62 COSMIC
COSN26644342 63 COSMIC
COSN28538191 102 COSMIC
COSN20093230 112 COSMIC
COSN31601039 122 COSMIC
COSN32058045 323 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1391811982 6 dbSNP
rs777032713 12 dbSNP
rs1310403899 16 dbSNP
rs761999561 25 dbSNP
rs1232122076 29 dbSNP
rs1279678270 30 dbSNP
rs765500573 34 dbSNP
rs1213289427 36 dbSNP
rs750614176 38 dbSNP
rs1477426796 41 dbSNP
rs540100674 42 dbSNP
rs932717310 44 dbSNP
rs201471345 47 dbSNP
rs772448613 53 dbSNP
rs553111230 57 dbSNP
rs1285124623 59 dbSNP
rs573154254 61 dbSNP
rs1303302109 64 dbSNP
rs1331675457 80 dbSNP
rs1286642465 88 dbSNP
rs1051222533 89 dbSNP
rs1232446592 95 dbSNP
rs1322926474 101 dbSNP
rs56889377 102 dbSNP
rs1355270054 106 dbSNP
rs373765497 112 dbSNP
rs1288704018 118 dbSNP
rs1431958052 123 dbSNP
rs59912867 123 dbSNP
rs796907245 123 dbSNP
rs1173523914 126 dbSNP
rs1037214006 131 dbSNP
rs1185217676 133 dbSNP
rs1429450367 134 dbSNP
rs1178496162 135 dbSNP
rs1269922304 151 dbSNP
rs190037588 153 dbSNP
rs1198801513 175 dbSNP
rs544037322 177 dbSNP
rs181253239 187 dbSNP
rs528707904 195 dbSNP
rs1022615683 197 dbSNP
rs1838498 199 dbSNP
rs1460922556 216 dbSNP
rs1159448151 217 dbSNP
rs1360194891 218 dbSNP
rs1838499 221 dbSNP
rs1295536717 241 dbSNP
rs186231930 243 dbSNP
rs1364540762 245 dbSNP
rs1300698923 247 dbSNP
rs1451550488 249 dbSNP
rs1002575149 257 dbSNP
rs1338191888 260 dbSNP
rs1463627806 262 dbSNP
rs1372392679 265 dbSNP
rs1035368938 266 dbSNP
rs1430027380 269 dbSNP
rs955803849 269 dbSNP
rs1461074415 270 dbSNP
rs540485757 275 dbSNP
rs1391203856 281 dbSNP
rs2589697 282 dbSNP
rs2532473 284 dbSNP
rs1205595869 287 dbSNP
rs1010002958 292 dbSNP
rs1251188365 314 dbSNP
rs1205899932 317 dbSNP
rs1309629271 322 dbSNP
rs1043628 323 dbSNP
rs866469956 330 dbSNP
rs968653482 332 dbSNP
rs548994748 339 dbSNP
rs1443854517 349 dbSNP
rs975101096 351 dbSNP
rs1298670107 364 dbSNP
rs1463495996 381 dbSNP
rs1347875909 383 dbSNP
rs1162638033 385 dbSNP
rs1445047028 387 dbSNP
rs1314697291 389 dbSNP
rs1386019855 390 dbSNP
rs921274644 392 dbSNP
rs1417385848 397 dbSNP
rs569012213 400 dbSNP
rs1178250467 402 dbSNP
rs1223337470 409 dbSNP
rs1481935524 413 dbSNP
rs1290249755 428 dbSNP
rs953990765 443 dbSNP
rs1360627103 449 dbSNP
rs1489027768 462 dbSNP
rs986804948 469 dbSNP
rs1334661188 473 dbSNP
rs912620379 476 dbSNP
rs1251729037 479 dbSNP
rs1365354272 485 dbSNP
rs1302472700 486 dbSNP
rs1424996226 488 dbSNP
rs1441606236 493 dbSNP
rs1367278826 502 dbSNP
rs1179411429 503 dbSNP
rs1407353991 505 dbSNP
rs531414832 510 dbSNP
rs1363281412 511 dbSNP
rs1182560228 519 dbSNP
rs1037181945 521 dbSNP
rs1368799193 529 dbSNP
rs368032007 530 dbSNP
rs1323793772 533 dbSNP
rs1490612994 534 dbSNP
rs931572170 537 dbSNP
rs1202978513 543 dbSNP
rs189595231 551 dbSNP
rs1447435012 559 dbSNP
rs1320816905 563 dbSNP
rs1282544432 564 dbSNP
rs1405552490 569 dbSNP
rs906127323 577 dbSNP
rs1002465670 579 dbSNP
rs1370573251 596 dbSNP
rs1381462369 598 dbSNP
rs2589699 607 dbSNP
rs1455362123 610 dbSNP
rs3865258 613 dbSNP
rs1481076028 614 dbSNP
rs1271870921 616 dbSNP
rs1009970517 631 dbSNP
rs1021452539 660 dbSNP
rs1322670588 663 dbSNP
rs968997708 666 dbSNP
rs567049141 667 dbSNP
rs1446787616 669 dbSNP
rs1271822074 673 dbSNP
rs535884241 675 dbSNP
rs996029118 678 dbSNP
rs1029289535 692 dbSNP
rs10886 699 dbSNP
rs71372292 700 dbSNP
rs1479723097 716 dbSNP
rs1325533763 718 dbSNP
rs1268376474 719 dbSNP
rs986773951 723 dbSNP
rs1326820812 724 dbSNP
rs181910301 739 dbSNP
rs912589452 743 dbSNP
rs1257199827 751 dbSNP
rs1403965652 760 dbSNP
rs1336103204 765 dbSNP
rs1470360332 768 dbSNP
rs1431995910 773 dbSNP
rs1166675085 779 dbSNP
rs1474756089 783 dbSNP
rs961440074 788 dbSNP
rs1374060607 790 dbSNP
rs1194837202 795 dbSNP
rs1442448177 800 dbSNP
rs1242169794 808 dbSNP
rs1203704021 816 dbSNP
rs1462272769 838 dbSNP
rs1162450015 840 dbSNP
rs1270730490 840 dbSNP
rs1271759376 844 dbSNP
rs973262010 844 dbSNP
rs1380800968 852 dbSNP
rs1314633297 855 dbSNP
rs1453388262 857 dbSNP
rs1425530774 858 dbSNP
rs1327430712 861 dbSNP
rs62075110 866 dbSNP
rs1397630729 871 dbSNP
rs1169959432 873 dbSNP
rs1423164142 878 dbSNP
rs1169690724 880 dbSNP
rs1352225422 883 dbSNP
rs952453841 883 dbSNP
rs1449642639 892 dbSNP
rs1180966357 896 dbSNP
rs1044583821 898 dbSNP
rs927526538 907 dbSNP
rs938962075 911 dbSNP
rs1358476366 915 dbSNP
rs1291298255 917 dbSNP
rs1378075853 921 dbSNP
rs544270647 921 dbSNP
rs1277904713 957 dbSNP
rs1441593659 960 dbSNP
rs112471989 968 dbSNP
rs1336828972 969 dbSNP
rs1243285141 977 dbSNP
rs1412542362 979 dbSNP
rs1310394622 982 dbSNP
rs1291757313 986 dbSNP
rs1320469253 990 dbSNP
rs1423069033 1003 dbSNP
rs891497892 1004 dbSNP
rs1219751062 1005 dbSNP
rs1272985737 1010 dbSNP
rs1305826916 1015 dbSNP
rs1009987173 1016 dbSNP
rs1157436436 1020 dbSNP
rs1212418523 1030 dbSNP
rs1197257376 1033 dbSNP
rs1043163061 1038 dbSNP
rs1238132133 1045 dbSNP
rs1269381207 1055 dbSNP
rs1485436015 1058 dbSNP
rs1323678246 1063 dbSNP
rs1186108062 1077 dbSNP
rs1282459986 1087 dbSNP
rs1223226062 1094 dbSNP
rs1351513592 1095 dbSNP
rs904281595 1097 dbSNP
rs1403712940 1106 dbSNP
rs1345242338 1112 dbSNP
rs1304186745 1114 dbSNP
rs1265015292 1115 dbSNP
rs1343000351 1124 dbSNP
rs1317209028 1128 dbSNP
rs1400767587 1131 dbSNP
rs1478046518 1134 dbSNP
rs996381822 1135 dbSNP
rs557547310 1142 dbSNP
rs1028842347 1146 dbSNP
rs890358555 1148 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 51030.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM714644. RNA binding protein: AGO2. Condition:completeT1 ...

- Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' agacucCGAC-ACCCCGAc 5'
                || | ||||||| 
Target 5' uuuccaGCAGUUGGGGCUa 3'
 		12 - 30
Article - Kishore S; Jaskiewicz L; Burger L; Hausser et al.
- Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
CLIP-seq Support 1 for dataset GSM714644
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repA
Location of target site ENST00000307767.8 | 3UTR | UUUUUAUUUCCUUUCCAGCAGUUGGGGCUAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
64 hsa-miR-4323 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT052834 NOL11 nucleolar protein 11 1 1
MIRT082489 PPP1R37 protein phosphatase 1 regulatory subunit 37 2 2
MIRT117166 PER1 period circadian clock 1 2 2
MIRT157951 TMEM184B transmembrane protein 184B 2 2
MIRT286013 TVP23B trans-golgi network vesicle protein 23 homolog B 2 2
MIRT310457 REST RE1 silencing transcription factor 2 2
MIRT338984 CPSF6 cleavage and polyadenylation specific factor 6 2 2
MIRT404223 RPL7L1 ribosomal protein L7 like 1 2 2
MIRT451439 TJP3 tight junction protein 3 2 2
MIRT463541 ZBTB7A zinc finger and BTB domain containing 7A 2 2
MIRT466981 STARD7 StAR related lipid transfer domain containing 7 2 4
MIRT479627 CDC25A cell division cycle 25A 2 2
MIRT487983 SEC14L3 SEC14 like lipid binding 3 2 4
MIRT488661 ELAVL3 ELAV like RNA binding protein 3 2 2
MIRT489925 RTKN rhotekin 2 2
MIRT490209 PKNOX2 PBX/knotted 1 homeobox 2 2 2
MIRT494896 ZNF662 zinc finger protein 662 2 2
MIRT495380 RELT RELT, TNF receptor 2 2
MIRT495549 ANKRD65 ankyrin repeat domain 65 2 2
MIRT499104 AGRN agrin 2 2
MIRT500036 ABCF2 ATP binding cassette subfamily F member 2 2 8
MIRT501135 SLC2A1 solute carrier family 2 member 1 2 2
MIRT505573 SMC1A structural maintenance of chromosomes 1A 2 6
MIRT508164 ABCC5 ATP binding cassette subfamily C member 5 2 8
MIRT512383 PHB2 prohibitin 2 2 2
MIRT512719 YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta 2 2
MIRT520904 STMN1 stathmin 1 2 2
MIRT526361 TIMMDC1 translocase of inner mitochondrial membrane domain containing 1 2 2
MIRT526847 GALNT11 polypeptide N-acetylgalactosaminyltransferase 11 2 2
MIRT526945 CMSS1 cms1 ribosomal small subunit homolog (yeast) 2 4
MIRT527028 PABPN1L poly(A) binding protein nuclear 1 like, cytoplasmic 2 2
MIRT527988 TSLP thymic stromal lymphopoietin 2 2
MIRT532202 MPDU1 mannose-P-dolichol utilization defect 1 2 2
MIRT533691 TMEM86A transmembrane protein 86A 2 2
MIRT535020 PRMT7 protein arginine methyltransferase 7 2 4
MIRT535054 PQLC1 PQ loop repeat containing 1 2 2
MIRT538987 BCL2L2 BCL2 like 2 2 2
MIRT552232 SART3 squamous cell carcinoma antigen recognized by T-cells 3 2 2
MIRT553259 TVP23C trans-golgi network vesicle protein 23 homolog C 2 2
MIRT555157 PTPDC1 protein tyrosine phosphatase domain containing 1 2 2
MIRT565202 TSC22D4 TSC22 domain family member 4 2 2
MIRT566199 PTP4A1 protein tyrosine phosphatase type IVA, member 1 2 2
MIRT566526 PARP16 poly(ADP-ribose) polymerase family member 16 2 2
MIRT568464 ARPP19 cAMP regulated phosphoprotein 19 2 2
MIRT572231 ATG2A autophagy related 2A 2 2
MIRT573096 NDUFA7 NADH:ubiquinone oxidoreductase subunit A7 2 2
MIRT573326 RFC5 replication factor C subunit 5 2 2
MIRT632802 KIAA2022 neurite extension and migration factor 2 2
MIRT646433 ARAP1 ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 2 3
MIRT647826 MTG1 mitochondrial ribosome associated GTPase 1 2 4
MIRT652191 TRIM44 tripartite motif containing 44 2 2
MIRT653800 SIRPA signal regulatory protein alpha 2 2
MIRT666949 PKHD1 PKHD1, fibrocystin/polyductin 2 2
MIRT684833 SCAI suppressor of cancer cell invasion 2 2
MIRT687294 PARP2 poly(ADP-ribose) polymerase 2 2 2
MIRT700188 RIMKLB ribosomal modification protein rimK like family member B 2 2
MIRT701784 MSL2 MSL complex subunit 2 2 2
MIRT705958 ACY1 aminoacylase 1 2 2
MIRT705998 ABHD14A-ACY1 ABHD14A-ACY1 readthrough 2 2
MIRT717107 PXDC1 PX domain containing 1 2 2
MIRT717586 VTA1 vesicle trafficking 1 2 2
MIRT722148 SLC2A8 solute carrier family 2 member 8 2 2
MIRT723479 WIPF2 WAS/WASL interacting protein family member 2 2 2
MIRT725221 PEA15 phosphoprotein enriched in astrocytes 15 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4323 Cisplatin 5460033 NSC119875 approved resistant High Hypopharyngeal Cancer cell line (FaDu)
hsa-mir-4323 Paclitaxel 36314 NSC125973 approved sensitive cell line (W1)
hsa-mir-4323 Doxorubicin 31703 NSC123127 approved sensitive cell line (W1)
hsa-mir-4323 Methotrexate 126941 NSC740 approved sensitive cell line (W1)
hsa-mir-4323 Topotecan 60699 NSC609699 approved sensitive cell line (W1)
hsa-mir-4323 Vincristine 5978 approved sensitive cell line (W1)
hsa-mir-4323 Docetaxel+Cisplatin+5-Fluorouracil resistant tissue (hypopharyngeal squamous cell carcinoma)
hsa-miR-4323 Tripterygium wilfordii Hook F resistant tissue
hsa-miR-4323 Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)
hsa-miR-4323 Neoadjuvant chemotherapy resistant tissue (breast cancer)
hsa-miR-4323 Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-4323 Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR4)

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