miRTarBase - #MIRT438298


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pre-miRNA Information
pre-miRNA mmu-mir-16-1   
Genomic Coordinates chr14: 61631880 - 61631972
Description Mus musculus miR-16-1 stem-loop
Comment None
RNA Secondary Structure
pre-miRNA mmu-mir-16-2   
Genomic Coordinates chr3: 69009902 - 69009996
Synonyms Mirn16, Mirn16-1, miR-16, mir-16-1, Mir16-1, Mirn16-2, mir-16-2, Mir16-2
Description Mus musculus miR-16-2 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-16-5p
Sequence 17| UAGCAGCACGUAAAUAUUGGCG |38
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B3XID9 miR-16 Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Urine Quantitative real-time reverse transcription PCR
B08JQH miR-16-5p Predictive Biomarker (PRD) Clinical/Experimental Data Expression . Peripheral blood MicroRNA sequencing
Gene Information
Gene Symbol Bcl2l2   
Synonyms AW048834, Bcl-w, Gtrgal2, Gtrosa41, bclw, c98
Description BCL2-like 2
Transcript NM_007537   
Expression
Putative miRNA Targets on Bcl2l2
3'UTR of Bcl2l2
(miRNA target sites are highlighted)		 
>Bcl2l2|NM_007537|3'UTR
   1 GAAAGTCTAGGGCCAGGTGGGGCTAGGTGTGGCTAGGGGCCAGGAGAGCTGGAACAATAGCGAATGCCCTTGGAAGAAAC
  81 TGGATGGTCACGGAACAGAGAAAGGCAGGGGAAGGTAGTGTGTGTGGGAGCCCAGCATGCCAGGCAGGTGGCAGGGAGAA
 161 TGAGTTGGAAACATTAAGGAATGTTTCAAGGCCAAGAACCCAGAGAGTGGAGTCATCCCACGGCTTGGGGGGTGGGGGTG
 241 GGGAGGTGGGAGGAATCATGCCCATATCGCGGGCACACAACTACCCGGAACATGGCTTGTAGCTCCAAGGACGGTGAGCT
 321 TACTTCAAAGATCTGTGTCTCGACTAGATGAATGGGATTTAGGGAGCCTAGAATTCACTTCCCTTTGGGATGGAAGCTTG
 401 GTGATCAGGTGACTGGGTGAAATGGCTGTGGCTGTGGGCTGCATGGGCACACCTGTGCATGTATGTGCACATGCAAGCAT
 481 GCTCATGTGCATGCTGGGCTGTCTGTCGAATCTGGTGGTGGGGTACTTAGAGAAAACATTCCTTCTTGCTATGGCAAGAA
 561 CAAGGGCCAGTTCACTGCCCTTCTCCTCTCTGCCCCTTCCCTTGACCTTACGCCTCCAGGCTGAGGGAGGAACACTGTAT
 641 CTAGACTAGGGCTCTGGTGCTGCCGAGAGTTGTTGGCAGGGCTTTGGAGAGAAGAGTTCTGCAGCTGGCCTTGTTCTTCA
 721 TCATCCCCCTGTTGTGTGCATCTGTGCACCTGGGCTGAAAAAGGGTAAGGCTGTGGAGCCTGGGTGGGTAGAAGCTTTGG
 801 TAGTTGGACTTGCCTGTCACCTTCCCCTCCAACGGTGGCACAATGGTGCCTAAAGTGTTGCCTGTTTCTAGGGTCTCTCT
 881 ATCCAGCTGAGTCTAAATTGGGGATCTAGTTTTCCTTTAGAAGATACGGGCATAAATGCTGATCTACAATTCAGTTTAGT
 961 TCCTGAACTGTCTCAGTGAGACTGCGGATACCTTGAGAGGACCATTCCAGCTCTGAGTCCCTTGGGAGTGATGGGGACAG
1041 GTACTCCTTGACTGGCCTGTTCTGGGTGGTGGGTTTTTGGTGCTGCTGCTCTATCAGGGGCCAGGGATATGGGTTCTAGA
1121 GTACCTGCCATGACCTAACGCGTTTTCTTTTGAAGCCATGCTCTTTGCATAGGTTGTCAACACTCGAGTCTCTGAAGATG
1201 GTCACATTAGAACTCACAGTCCAGTCCCCTAGAACAAATCCAGACTCTAGCCTTTTCTTTTGAGGGAGGAAATGATTCAC
1281 TCCAAATGCATGCCCTGAGCCAGACCTCACTGCTGCACTTCCCAAGGTGCTAACATTGCTGCTCTTTAATGCTGACTTCG
1361 TTCCCAGTGCTCTAGAACCCTACCTGCCTCTCCTGAGCACTGACCAGCTTGGCTAGACCACAGCTGACCCACCTCTTCAC
1441 TTGGTCCTAGAATGTGGCATCATATCGTGCTTGCTAGAATGTGGGACCAAATTGGCCTCAGGTGTTTTAGTCCAGACTTT
1521 GGCTTTTGAGAGAAGGTTGCACTTTTTCATAGTGTCTATGGGGGATGTGGGAGGCTGCATGTGCCAGTTGGTCTGTTTTG
1601 AGGGTGATGATCTAAGAGACTCGGAGCTGTCTGTGACAAGCAGTCAGAGTGGGAGGCTCTGACTTGCTCAGGACAAACTA
1681 GGCCAGTGGTTTTCAAACTGCTTGGCAGAGCCCCGAAGTGTCCTAGGGGTTGCCTCAGGAGTCCTTAGGGAGATGAGGGA
1761 GTGGAGGGCTGGGCAGTTTGCCCTCAAACAGAACAGCTCCCCCTTGTAGCTGTCTTATGTATCGGGGTTCAGGGTAAGAT
1841 TTTATTTGTGGTAAGGGCTTTGCTGCTGAAAAAAGTTAGAAAACCACTGACTAGACCATCAGCTCCATCTGGAGCCTGTG
1921 CTCCTCCACGTCCAGGGAAGTCTCTGCTCTACCCTTCCCATGGGACACCTACCTAAGGTAGCATTCCTGGGGCCCTTTAG
2001 CAAACAGGAGCAGCAGGAACCAGGGTCAGGTTACCAACATGCTCTGCTAGCAGACCTTCAGCCAGGTGGGAGGAGAGGCT
2081 GTGCTAGGACAGGCTAAAGGGTTGGTCTTGGTCTGGATTCTCAGAAGCTTAGATGGGACCACAGTGGAGAGTTGTGACCT
2161 GTTCTGCCCTTCGTTTTAAGGGCCAGTGGAAACCCAGAGACTCTTCTGTCAGGGAAAACCAGGGACTGTCTTTTAGAGCC
2241 ATATAGTTCCTTGGGATTAGCTCTTGGCCAAGAAGGCTGAGTATGGCTCCCAACTTTTAAATCCATTTCATTTTTTTTAA
2321 ATGAGGGAAATGGATATAATTTTTCAGATACTAAGTAGCTGGAGAGGATGTTCTTGCTCTCCAAAGCCCAAAGGGACAAA
2401 TAGGGACTTTGCTTAGGCCAAGGCAAGAGCGCAAGTGGGCACTCAGTCCTGCAGTTACCAGTCCTACTCCCCACTTACAC
2481 TAGGGCATACATATACTATTTTACTTTTTTAAATCATAAACGGCAGGAGAACAGATTTGGTTAGTTTAGAAGAAAAGAAA
2561 AAGCTCTATAAATATAAATATATATTCCTGTATTTTTATTTAATAATTTATAAATGCCAAGTTCATTTGACTTTTATTTT
2641 TGTGTAATATGTAATGGTCGTATTAAAAATAAATAAATAAAGCCCAGAAATTTAATGAGAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gcggUUAUAAAUGCACGACGAu 5'
              ::| |||: |||||||| 
Target 5' ggtgGGTTTTTG-GTGCTGCTg 3'
 1068 - 1088 157.00 -16.10
2 
miRNA  3' gcGGUU--AUAAAUG-CACGACGAu 5'
            ||||  |: | ||  ||||||| 
Target 5' tcCCAAGGTGCTAACATTGCTGCTc 3'
 1320 - 1344 154.00 -11.40
3 
miRNA  3' gcgguuAUAAAUGC----------ACGACGAu 5'
                |||||::|          ||||||| 
Target 5' agatttTATTTGTGGTAAGGGCTTTGCTGCTg 3'
 1837 - 1868 146.00 -12.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions 293TN
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... 3UTR of Bcl-w contains conserved miR-15 family binding sites. ...

- Yu B; Gong M; He Z; Wang YG; Millard RW; et al., 2013, The international journal of biochemistry & cell biology.

Article - Yu B; Gong M; He Z; Wang YG; Millard RW; et al.
- The international journal of biochemistry & cell biology, 2013
UNLABELLED: We reported previously that pre-programming mesenchymal stem cells with the GATA-4 gene increases significantly cell survival in an ischemic environment. In this study, we tested whether regulation of microRNAs and their target proteins was associated with the cytoprotective effects of GATA-4. METHODS AND RESULTS: Mesenchymal stem cells were harvested from adult rat bone marrow and transduced with GATA-4 (MSC(GATA-4)) using the murine stem cell virus retroviral expression system. Cells transfected with empty vector (MSC(Null)) were used as controls. Quantitative real-time PCR data showed that the expression levels of miR-15 family members (miR-15b, miR-16, and miR-195) were significantly down-regulated in MSC(GATA-4). The protein expression of Bcl-w (Bcl-2-like-2), an anti-apoptotic Bcl-2 family protein, was increased in MSC(GATA-4). Hypoxic culture (low glucose and low oxygen) induced the release of lactate dehydrogenase from mesenchymal stem cells and reduced cell survival. Compared to MSC(Null), MSC(GATA-4) showed less lactate dehydrogenase release and greater cell survival following 72 h hypoxia exposure. The mitochondrial membrane potential, detected with the dye JC-1, was well maintained, and mitochondrial membrane permeability, expressed as caspase 3 and 7 activities in response to the ischemic environment was lower in MSC(GATA-4). Moreover, transfection with miR-195 significantly down-regulated Bcl-w expression in mesenchymal stem cells through a binding site in the 3'-UTR of the Bcl-w mRNA and reduced mesenchymal stem cell resistance to ischemic injury. CONCLUSIONS: The overexpression of GATA-4 in mesenchymal stem cells down-regulates miR-15 family members, causing increased resistance to ischemia through the up-regulation of anti-apoptotic proteins in the Bcl-2 family.
LinkOut: [PMID: 24070634]
51 mmu-miR-16-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001064 Wnt3a wingless-type MMTV integration site family, member 3A 4 1
MIRT001065 Ccnd1 cyclin D1 4 2
MIRT001066 Bcl2 B cell leukemia/lymphoma 2 4 2
MIRT001689 Ccnt2 cyclin T2 1 1
MIRT003375 Arl2 ADP-ribosylation factor-like 2 4 1
MIRT004105 Cadm1 cell adhesion molecule 1 2 1
MIRT005352 App amyloid beta (A4) precursor protein 4 1
MIRT005922 Mdm4 transformed mouse 3T3 cell double minute 4 3 1
MIRT005923 Vegfa vascular endothelial growth factor A 4 3
MIRT005924 Jun jun proto-oncogene 3 1
MIRT005925 Jag1 jagged 1 3 1
MIRT006566 Slc6a4 solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 1 1
MIRT006940 Ccne1 cyclin E1 3 2
MIRT427938 Ppap2b phospholipid phosphatase 3 3 1
MIRT438298 Bcl2l2 BCL2-like 2 1 1
MIRT438763 Cd40 CD40 antigen 3 1
MIRT438766 Fgf2 fibroblast growth factor 2 3 1
MIRT577813 Rnf168 ring finger protein 168 1 1
MIRT578440 Irgq immunity-related GTPase family, Q 1 5
MIRT578972 Dhdh dihydrodiol dehydrogenase (dimeric) 1 2
MIRT583721 Erlin2 ER lipid raft associated 2 1 1
MIRT584146 Creb5 cAMP responsive element binding protein 5 1 1
MIRT591285 Klc1 kinesin light chain 1 1 2
MIRT591939 Ddx19b DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b 1 1
MIRT592081 Sorcs2 sortilin-related VPS10 domain containing receptor 2 1 1
MIRT592201 Mapkap1 mitogen-activated protein kinase associated protein 1 1 2
MIRT592347 Armcx6 armadillo repeat containing, X-linked 6 1 2
MIRT592355 Angel1 angel homolog 1 1 1
MIRT592370 4930444A02Rik protein-O-mannose kinase 1 1
MIRT592407 Tacc1 transforming, acidic coiled-coil containing protein 1 1 2
MIRT592431 Spsb4 splA/ryanodine receptor domain and SOCS box containing 4 1 1
MIRT592665 Itgav integrin alpha V 1 1
MIRT592716 Fbxo21 F-box protein 21 1 2
MIRT592808 Bicd1 bicaudal D homolog 1 (Drosophila) 1 3
MIRT597924 Pacsin2 protein kinase C and casein kinase substrate in neurons 2 1 1
MIRT598104 Ncl nucleolin 1 1
MIRT598638 Idua iduronidase, alpha-L- 1 1
MIRT599012 Fgd4 FYVE, RhoGEF and PH domain containing 4 1 1
MIRT600261 Trim2 tripartite motif-containing 2 1 1
MIRT600294 Tifab TRAF-interacting protein with forkhead-associated domain, family member B 1 1
MIRT601126 Bri3bp Bri3 binding protein 1 1
MIRT601137 Bcl11b B cell leukemia/lymphoma 11B 1 1
MIRT603793 Lpcat2b lysophosphatidylcholine acyltransferase 2B 1 1
MIRT733369 Synrg synergin, gamma 1 0
MIRT733370 Tnrc6b trinucleotide repeat containing 6b 1 0
MIRT733372 Lamtor3 late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 1 0
MIRT733373 Hip1 huntingtin interacting protein 1 1 0
MIRT733374 Il6ra interleukin 6 receptor, alpha 1 0
MIRT735570 CXCL10 C-X-C motif chemokine ligand 10 3 0
MIRT736046 Il7r interleukin 7 receptor 2 0
MIRT756040 Rbfox2 RNA binding protein, fox-1 homolog (C. elegans) 2 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-16 cisplatin approved 84093 Quantitative real-time PCR K562 cell 20428827 2010 up-regulated
miR-16 Cisplatin approved 84093 Quantitative real-time PCR SH-SY5Y cells 24026226 2013 up-regualted
miR-16 Benzo(a)pyrene NULL 2336 Microarray MM plasma cells 24798859 2014 up-regulated
miR-16 Calcium sulfate (CaS) NULL 24497 Microarray MG63E osteoblast-like cells 17618507 2008 down-regulated
miR-16 Glucocorticoid NULL NULL Microarray acute lymphoblastic leukemia 19148136 2009 up-regulated
miR-16 Glucocorticoid NULL NULL Northern blot acute lymphoblastic leukemia 19148136 2009 up-regulated
miR-16 Glucocorticoid NULL NULL Quantitative real-time PCR acute lymphoblastic leukemia 19148136 2009 up-regulated
miR-16 5-aza-2'-deoxycytidine (5-Aza-CdR) approved 451668 Microarray Pancreatic Cancer PANC-1 cells 19407485 2009 up-regulated
miR-16 5-aza-2'-deoxycytidine (5-Aza-CdR) + trichostatin A(TSA) NULL NULL Microarray Pancreatic Cancer PANC-1 cells 19407485 2009 up-regulated
miR-16 Trichostatin A (TSA) NULL 444732 Microarray Pancreatic Cancer PANC-1 cells 19407485 2009 up-regulated
miR-16 All-trans-retinoic acid (ATRA) approved 444795 Quantitative real-time PCR acute promyelocytic leukemia (APL) 19749800 2009 up-regulated
miR-16 Curcumin NULL 969516 Quantitative real-time PCR MCF-7 cells 19908170 2010 up-regulated
miR-16 Glucose NULL 5793 Microarray proximal tubule cell line HK-2 20067797 2010 down-regulated
miR-16 Bisphenol A NULL 6623 Microarray immortalized cytotrophoblast cell lines HTR-8 20417706 2010 up-regulated
miR-16 Polylysine NULL 162282 Quantitative real-time PCR 293T(FLAG AGO2) cells 20529860 2010 down-regulated
miR-16 Trypaflavine NULL NULL Quantitative real-time PCR 293T(FLAG AGO2) cells 20529860 2010 down-regulated
miR-16 Nicotine approved 89594 Quantitative real-time PCR gastric cancer cells 21081469 2011 up-regulated
miR-16 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-16 Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-16 Ginsenoside Rh2 NULL 119307 Microarray human glioma cells U251 21372826 2011 down-regulated
miR-16 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 up-regulated
miR-16 5-Fluorouracil approved 3385 Quantitative real-time PCR MCF-7 breast cancer cells 21506117 2011 up-regulated
miR-16 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-16 Estrogen NULL NULL Quantitative real-time PCR breast cancer cells. 22260523 2012 down-regulated
miR-16 Cisplatin approved 84093 Quantitative real-time PCR HeLa cells 22475935 2012 up-regulated
miR-16 Bortezomib approved 387447 Quantitative real-time PCR bone marrow stromal cells (BMSCs) 22781767 2012 up-regulated
miR-16 Melphalan approved 460612 Quantitative real-time PCR bone marrow stromal cells (BMSCs) 22781767 2012 up-regulated
miR-16 Galactose NULL 6036 Quantitative real-time PCR lens 22736950 2012 up-regulated
miR-16 Tamoxifen approved 2733526 Microarray rat liver 17343880 2007 up-regulated
miR-16 Tamoxifen approved 2733526 Quantitative real-time PCR rat liver 17343880 2007 up-regulated
miR-16 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 up-regulated
miR-16 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 up-regulated
miR-16 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 down-regulated
miR-16 Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 down-regulated
miR-16 Morphine approved 5288826 Microarray human monocyte-derived macrophages (h-mdms) infection with HIV-1 20564181 2010 up-regulated
miR-16 Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-16-5p Tamoxifen 2733525 NSC180973 approved sensitive Low Breast Cancer tissue and cell line
mmu-miR-16-5p Vincristine 5978 approved sensitive Low Gastric Cancer tissue and cell line (NF-3xmir16)
mmu-miR-16-5p Doxorubicin 31703 NSC123127 approved sensitive Low Gastric Cancer tissue and cell line (SGC7901)

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