miRTarBase - #MIRT438818


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pre-miRNA Information
pre-miRNA mmu-mir-7a-1   
Genomic Coordinates chr13: 58392779 - 58392886
Description Mus musculus miR-7a-1 stem-loop
Comment miR-7a (previously named miR-7) was predicted by computational methods using conservation between mouse, human and Fugu rubripes sequences . The ends of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-7a-1-3p
Sequence 66| CAACAAAUCACAGUCUGCCAUA |87
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Pfn2   
Synonyms Pfn
Description profilin 2
Transcript NM_019410   
Expression
Putative miRNA Targets on Pfn2
3'UTR of Pfn2
(miRNA target sites are highlighted)		 
>Pfn2|NM_019410|3'UTR
   1 CTGCTAGGCAGACTGTTAAGTATTAGGGGAAAATTGCTCTTAAATTTTCCTAGCTGTAAGCTTAAGTCTCCTTTGTATCC
  81 CTCTGTTGGTGGGGAAAGGTGTCTTTCTTTCTGCCCCTTCCCCTTAAATAATTGTGTTCATTTTTGTTTTGTTTCCTTGT
 161 GTACTCCAGCATTGGTTATAGTCATGGGAAAGGAAGGTGTCCACGCAGGCACAATTAACAAGAAAACATATGAACTCGCT
 241 TTATACCTGAAGAGGTCTGTAACCAACCTTTATCTGGCATCATAATTGCAGCACAATAATGATTTGCATGATATCTTGAA
 321 ACTGGGGGAAGGGGGCATGCCAAGTTGGGCATCACTTCGTCTTAGCAGTTAGTGGATTACTGATTACTAAAATAGGTTAA
 401 TAGTAAGCAAGGTGCCGGTGTACAGTCTCTAACTCGATCAGTGTCTTTTCAGCACTTTGGAGCATTTCCTTGGCTCATCT
 481 AGTCTTCCTTTTGTAGCGCATGGTTGGGAGGAAAAAGTGCATGCCTCTGTACGTCACACCTTTCTCACCCCTCCCTACCA
 561 ACACGAGGTGTTTGGTCTGCTTCCATTCTCTTCTGTGTAGTGCCTGGTTTATTTAACTAATTAATACCTCCTCTGTTGAT
 641 GAGCTATTGAGAGTTGCAGTGTTTTACTTTTTAATACAGTGACTGCACTTGGGACAGACTATTTGTAGTGTCTATAGGAC
 721 ATGAAGCGTGCAGCTTGGAGAGCAAAGGCACTTCCTCCCACGACCTTACTGCAGTAACCATACCGATCAAATTCCCAGGG
 801 ACATTCTGTCATTGCAGTAGCTCAGACCTGTCTTTTCTTGCACACCAGCTGTGCTCTTGAGAAAATGTAAAGGCTGCCAT
 881 TATGGATATTAGGTATCCCAACATAACCATCTGGAGTGTGTCCAGTTTATTTTTCATAGGACCAATTTTTATTTGCAGCT
 961 TAGGTTTTTATATGAAGTTGCATTATTGTGGACTTGGCTGTCTTGTGATGTATTTTTTTCATATGTATCCTGTGCCATAC
1041 TATTGTTAAAATGAACTGTTGCTATTGTGAGATGGATTTCCTTTCGAATGGCACTACCTTAGGGACATTCTAGTATTGCT
1121 TCTATTGTTTGGGCATTGTGGATAATGTACAGATTTAAAAACAAATCTTGTTGCTGATTTGTCCATTTCTTTCCCTGCAC
1201 TTTGTTACATCTGGGATACAGTCTAACTCATCTGACTTAATATGCATTTAAAAAAATGCCATAACTATTAAACACCTTGT
1281 TTACAGACAGATGAAATAAATTTATTCCAACCAAATACTCCA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auACC--GUCUGACACUAAACAac 5'
            |||  ||||||   ||||||  
Target 5' ctTGGGACAGACT---ATTTGTag 3'
 688 - 708 143.00 -12.70
2 
miRNA  3' auaccgucUGAC-ACUAAACAac 5'
                  ::|| ||||||||  
Target 5' caaatcttGTTGCTGATTTGTcc 3'
 1162 - 1184 134.00 -7.80
3 
miRNA  3' auACCGUCUG-ACAC----UAAACAAc 5'
            |||| | | ||||    |||| || 
Target 5' ctTGGCTGTCTTGTGATGTATTTTTTt 3'
 993 - 1019 130.00 -10.40
Experimental Support 1 for Non-Functional miRNA-Target Interaction
miRNA:Target xx
Validation Method
     
Conditions MIN6 cells , HEK293 cells
Disease CCCCTCGAGCAGCCAAGTCCAC AATAATGC; 18645.0
Location of target site 3'UTR
Tools used in this research microarray
Original Description (Extracted from the article) ... "MicroRNA-7a regulates pancreatic 脦虏 cell function we determined that miR-7 is a negative regulator of GSIS in 脦虏 cells. Using Mir7a2 deficient mice "Mutations of miR-7 sites in selected targets conferred complete derepression of luciferase activity demonstrated that direct miRNA engagement on the 3芒鈧虏-UTR of these transcripts was required for gene regulation by miR-7a. Together ...

- Latreille M; Hausser J; Stutzer I; Zhang Q; et al., 2014, The Journal of clinical investigation.

Article - Latreille M; Hausser J; Stutzer I; Zhang Q; et al.
- The Journal of clinical investigation, 2014
Dysfunctional microRNA (miRNA) networks contribute to inappropriate responses following pathological stress and are the underlying cause of several disease conditions. In pancreatic beta cells, miRNAs have been largely unstudied and little is known about how specific miRNAs regulate glucose-stimulated insulin secretion (GSIS) or impact the adaptation of beta cell function to metabolic stress. In this study, we determined that miR-7 is a negative regulator of GSIS in beta cells. Using Mir7a2 deficient mice, we revealed that miR-7a2 regulates beta cell function by directly regulating genes that control late stages of insulin granule fusion with the plasma membrane and ternary SNARE complex activity. Transgenic mice overexpressing miR-7a in beta cells developed diabetes due to impaired insulin secretion and beta cell dedifferentiation. Interestingly, perturbation of miR-7a expression in beta cells did not affect proliferation and apoptosis, indicating that miR-7 is dispensable for the maintenance of endocrine beta cell mass. Furthermore, we found that miR-7a levels are decreased in obese/diabetic mouse models and human islets from obese and moderately diabetic individuals with compensated beta cell function. Our results reveal an interconnecting miR-7 genomic circuit that regulates insulin granule exocytosis in pancreatic beta cells and support a role for miR-7 in the adaptation of pancreatic beta cell function in obesity and type 2 diabetes.
LinkOut: [PMID: 24789908]
7 mmu-miR-7a-1-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT438818 Pfn2 profilin 2 4 1
MIRT438823 Prkcb protein kinase C, beta 4 1
MIRT438845 Snca synuclein, alpha 4 1
MIRT438864 Zdhhc9 zinc finger, DHHC domain containing 9 4 1
MIRT438865 Wipf2 WAS/WASL interacting protein family, member 2 4 1
MIRT438867 Cplx1 complexin 1 4 1
MIRT438876 Basp1 brain abundant, membrane attached signal protein 1 4 1

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