miRTarBase - #MIRT438823


—
pre-miRNA Information
pre-miRNA mmu-mir-7a-1   
Genomic Coordinates chr13: 58392779 - 58392886
Description Mus musculus miR-7a-1 stem-loop
Comment miR-7a (previously named miR-7) was predicted by computational methods using conservation between mouse, human and Fugu rubripes sequences . The ends of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-7a-1-3p
Sequence 66| CAACAAAUCACAGUCUGCCAUA |87
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Prkcb   
Synonyms PKC-B, PKC-Beta, Pkcb, Prkcb1, Prkcb2
Description protein kinase C, beta
Transcript NM_008855   
Expression
Putative miRNA Targets on Prkcb
3'UTR of Prkcb
(miRNA target sites are highlighted)		 
>Prkcb|NM_008855|3'UTR
   1 GTAGATCCGTAGACCTCCGTCCTCCATTTCTGTCATTCAAGCGCAACAGCTGTCATGGTGACATTTTTTCTTTTTCATTG
  81 CCGAGTTACATCCGTGTTTTCTTTTCTGATGAGGCTAGAGTGGCCATGTTTCAGAACCCAAATGTCTTCAAGTAGTTTGG
 161 AGCATCTCAAGGAGATGGAATTATGCAGGTGGCATGTAGAAAGTGCTGCATAATCGAGACACGCTCGGAGTCCTACTATG
 241 TGCGTCCCTAGCATGTCGGCTCACAGACCCCCACGGGAGAGAAAATGCTTGCTTTTCTCCCTGGTCCTCTGCACTGCCAT
 321 CTTTACCCCAACCCTCCAGGCTACATCCTCCCTACAAAATGGATGGATGAACCAAATGCTAGCAATATCCTTCCACTTTC
 401 CAGGCTGGGAGCTTGGCTTGAATCCAAGTATATGGCTGCTTTGCCCTAGAGGGAATCCCTCCACGCTGCCTGTTCTGGAA
 481 GCAGTTGAGCTTTGAAAAGAACATGCTAAAAATAAATTTTTATTTTTATTTTTTTAACTCAGTGTTAAGAAGATTATCTA
 561 AGTCCTGGGGAAAAAAGTGGGGGGAATGCGCTTTTAGACAAGTTTCCATCTAAAAGCACTTCAGGGTCAAAAGGCAACCA
 641 GCTTGGGTGCTGTCACCTTGCCATAGTTTCTAATACTGTATGTCTGATAGCGGTACCTTCATCCCCCAAACTACTCAAGA
 721 ATGACATTTGGCACTGTTCCTGGAACCACATGGTCACTCTAGCCAGGTCCCAAAGGACCCTGATTCTACATTACATTTCA
 801 AATGTTACTTGCTTTTTATTTCTGTCATCATTGAAATGCAAAAAGAAAAAAGAAAAAAAAGAAAACGAAAGAAAAAGAAA
 881 AAAATACTCACTTTGTTACACATGCTTTAAGATCTGTGTCCAGATGTTATTAACCATAATGGCCTGCTTTGTTCAACCAA
 961 GAAGATGGCTATGGAACCCAGCAGACTTGTGCCCATGGGGAGGGGATTTGTCTAGATGTCCCACTGGTATTGGAGAGGTA
1041 ATTGTGTGCTCTGAGAAAGACGCCATCTCCCCAAACCATGATGGGTGCATTCTTCAGTATGATGTTATTTTGCAAGATGT
1121 TTGTGGGAATGTTTATTTGCACTTCAGTATCTGTTCTGATATCTTTCTCCTAGCATCAAGATACAATACAAAGAAAGAAA
1201 GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAGAGAGAGAGAGAGAAAGGAAAGGAAATCCTATTTCA
1281 AAGAAAACTGTTCCCTTTGAACAATGAGGACCCATACTACAGATTAGGGTCTAAAGAAGGAACTCAGGAGAAAGGAACTA
1361 AAGCTGGACCTTTAATAGCCTCAGCGTTAACCAGCCAGAGAGTAGAGCCCGGAGACTATTCAGAGATGCCTGATGTCCTG
1441 AAATGGGGTGGGGTAGACAGGCTGGAGCTTCCCACAGCATCAGACTTCAGAGACCACATTCCCATCAGAATCAAGCTTAG
1521 GAACATTCCCAAGGCTCTTAAACTTCTCATCAGAATCACCACTCTTACAAATTTTAGCATCCTTGAGCAAGCAACTCAAT
1601 GAGCTCCCAGAAGTCCAAGGGAGCCCTTGGAGCAGGAGAGACTTCATTTCTCCCTGAACAGATGGGCACAGCAGGTGGCC
1681 CCTTACCCTTGCCTACATCTTAGCACCCTGCTCCTCACACACAGTCTTGAGGCAAATTCTTATTACAGGCTAAAAAATGT
1761 CTGGGTCCTCCAGTGCTTCTGAGAGATCACACAGAAATGTCCAAAGGACCAGTGTCCTTGAAGTATACCTCCTCATCTTT
1841 AAGAAGACTATGCCTTTGGGTTCTTCCGAGGCAAAGACCCTTATAGGTCATTCTTTCCTGGCCTCTTAGCAACTCAGCTT
1921 TAAGCTTAGGCCTTGCCCTGCTTAGATCATGTGAAGCAAGCTTGTTGTCCTAACAATCTTACGATTTTAGCTGCCAAAAG
2001 AGACCAGAGCAATCCTGCTCCTCACTCTGCTATTAGGGCACCTGACATCTGAAGAGTAGAAGTTATTTTCTGCTTGGTTG
2081 ATCAGTGGTGATGCAAACACAAGTCTTGTGGCCAATGACACAGGACTCTGTTCTATAACAAAATAAATATCTCTGAGCCA
2161 CATGTCCATTCCCAGGCAAGTTATGTAGGGTCTTTCATTTGGTCAATGGGTCACCAGGCCTTGGACATATGCTCAGAATA
2241 AAAACCACAGAGAAATCTTGGGCCATTGGCAAGTGATCTGAGACGACCTTAGAAAACAGTGTAGGAAGGAAGGATGGTTA
2321 GATGGATGGATGGATGAATAGATAGATGGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAATAGTATATATG
2401 TAAATATATACACTACGCTCTACATTTTCAGGTTCTTACCATTATGTTTTAAGGTTCGTAGTTGTCAGAAATAGATACTC
2481 AAGCCAAAGTGTCTGTTCCCTTAGCTTAGCTTGATGGGGGTGGGTCAGAGAAGCTTCTGTGGAAATAATGGATCATGAAT
2561 CAGACCTCCCACTGCTTCCTCGGCCACCAGTGTTCTAGATCAGAGGAAGCCAAGAAGCAGTTTCCATCACGTAAGATCCT
2641 TAGGAAATATTTTAAAATTTTATCCAAAAAAGAAATAATTTGTTTTTTTTTTTCATCCATCCATTTATACATCTTCCATC
2721 CATTTGAGTAGCATTTATTGACCACCAACTGTGTGCCAGGCTGGGTGCCAGGATCCTGGTGAAAACTATGAAAGATGCAG
2801 TCCATGCCCTTCTCCCTCTTTCTCCAGCTCACCATCTGTGGAAAGATGGCCATAAAACAAGTAATTGAAAGTATGCTGTT
2881 GTCTAGAGTCTCTGAGCTTATTAAAAAATTTTTCTGCTGGAGGTCTTCCAGGCTTCTTAGTCTCTCTATAGACCATAGTT
2961 TGAAGCTATCAAGCCATAGTATCAAGCTCCATACTTGATCGGACCACAGACGCTCCCACAACATGAACGGTGCCCGTGTC
3041 TTATGGAGGAAGGGAACTAACTGGCTTTGTGACTTATCTCCGAGTCTTCAGTGTCAGCTGTCATTTTGCATCCATCACTT
3121 GGCTATCTGCAGGCCCTTGGGGAATACCATTCCAGTTTAGAGCACTGCTGATCAAATCATGTGACCCTTGCTGGTCACAT
3201 CCTGATCTCTCTAGCTCATCTGAAACAGGATTAAAATTTTCACACACACACACACTCACACACATATTTAACTTCTAGAA
3281 GAACTGAAGGAAAAAAACAAACTAAAAGAAATCTAACTAAAGCTGCTGTGAACTGTGACCAGTCATGTGTCTGGATCCAA
3361 GAATCCACTTTTAAATTTCATATATTTTTGGCTGATTTCCTCCTCTAAGAAAGGATAAGGCCATGATAGACCCAAGGCAG
3441 ACCGGTAGAATTAACTCTCTTCCTAGGATGGGAGTTTCTGGTCTCATGTGGCTTCAGATGCAAATTTCCAAAACGATTGT
3521 CACAACAAAGGAGCCCATTCCCTCCCAACCTTGTATGGTAAAATCCTGGAGTTTTCCTGGGAAGGTCCACTCATGAGATG
3601 AAACCCATGAAAAGGAAGTCTTATTTGAAACACATGGCTTACTGAAGCACGGCAAATAAAAAAGGCTTCCAAAGTTGGAG
3681 CTGGTAGGAAGGGAAGTCCTGCCACATGCTCCAAGTTAAGGATGCCTGGGTAGAAAGAGACCCCCAAAAGAGGAGCTATC
3761 TTCTGCCCCCAGGTCTGCCCTTCTCCTTTCCACACAAAGCCTTGTAGCTTCCAGACCCTGCTCATACATCGTTCACTCCA
3841 TACTGGCTCCATTTACATAAGTCTTAGGACCTGTAAAAATAACTGTTATCATTTTGCTTCAGTGTGAAGAGGAATCTGCC
3921 TCAGGAATTAGGAAAAGCAAGGAAGCGCCCCAGTAAGGAAGTCTTTTTTATCAATTAGATGAGTACTTATCAACAGCCTA
4001 TTGTGTGCTCAGTAGCACTGAAAACACAATAGCCCAGGAACAGACTAGGACCTTGCCCGAAAGGAGAAAAACAATGCAAA
4081 TTCCCCAAAGCAGGAAATGCCCAGAAGCTGAAGGGGCTGCAAACACATGGCTTATAGAATCAGGAAAGAGAAGACAGCAT
4161 TAAAGAGACAGAAGGCATCTGAGCTTCCCTCGGGAAGCAGAAAGTAACAGCATGGACAGCCTGGAACCCTCCACCAAAAC
4241 TCAGTATGGACAGGATACCACAAGAGAAGAGCCACCATGTAGGAAACGGCTTCATGGGTAGCCTTCGCCTTGTCTTCTAT
4321 ACCTATCCTCTTTCAGATGACCTCCTGATGCCAATTTGTCTCCCTCAACTCAGTCCCATAACCTTACTCATACACCCCAG
4401 TAGGAAGTACCTATCGCAGGAGACCATCCAGAAAGAAGTAGGTTAGAGCCTAGCAACAGGAAATTCATATCAGCCAGTCT
4481 CCGTTAGGGGCTAAACCTACTAAGATTGGCCAAAGTGAATCAGCCAACAAACTCTTCTGTAACACACCATGGCCTCAGCC
4561 AGGCACCTTACCTGTTGCTAGGAATCCCAGGCACTGTGAAAACAGTCTTCAGGCTTTCTCTCCAGCCAGAGTAGTTGTCA
4641 TGAGGCAAGAATCATCTGCTATGTGGTCAGATATAAGTCTCCAACCCGCTGCACAGGCTTGGCCAAGTCCCTTCACTGAC
4721 CTGTGCCCTGGTTTTCTCATATGTCCAACAGTGGGAGACAATCACATCAGCTTTGCCTTCTCCAGACCCGTGAGGTGAGA
4801 TTGGGAAGGGCTAGTAGGCTTACAGGAGCCCTGGCGAGGCCAGCACATCAAAAAGCCACCCACATTCACATTCTCTGGCA
4881 GAACGTGCCTCAAACTAGTCAGCAATTCCTGTACACATGCAGGCCAGCAGGCAGACCGGCAGGCAGGAAGAAAGGAAGGA
4961 AGGGAGAGAGGGAAGGAGGAAGGCAGGCAGGCAGGCAGGCAGGCAGGCAGGCTGGCCAGGTTACCTACTACAAGAGTTGA
5041 GCAGTTTTATTCTTCAAGCTTGCCTTCATACTTTAAGCTTTGTATAGAAGCACACCACCAGCATCTTTGGAGAGACTCTG
5121 GCTACTGAAGACCCAGATTCTACGCTAGTGTTCAGCTTTTGAGCAGCATTTCAGAGCCTAGCTTTCACCAATTTCTTTTG
5201 GGGGGCGGCTGAATCTAAAATATGAGTTAAGTAGACCCTAACGGGCTGGCCAACTCTGAGTCAGATCGTCTGACACAATT
5281 CTGGCACCAGCTAGGCTGTCTGTGGTCAGCAGTGAGTCTCAGACAGTATGAGGAAGAGGTAGATGGTGTTTAGCTTCTTG
5361 CAATGAACACTGCTTCCCTCACATGGCTATTTGCCTTCTGAGAGCAATGTGTATATACTCTCTCTCTCTCTCTCTCTCTC
5441 TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCTCTCTCTCTCTCTCCTCCCCCAACAAAGTCAGCTCCTGGCCAC
5521 AGGGGTCGCCATTGAGTTGTTTTCTTCCAGGATACTCTATCTAAGAGCCAAGTCTATGGCATTAGCTGTGAGAAGTAGTT
5601 ACCACTGTAATTCACCTCAAAGTCGAAGCATAGGACTCCCCCCCACACAAACACACATTTCTTTAGGGCAATTCTTAAAA
5681 AAAAAAAAAAAAAAAAAAAAGAGGATGAATCTTTTCTTCTCATAGAAAATTGGCACCAAGTTTCCTCCCCCAAGCCAGCA
5761 TATAACATGAACTGAGAGCTGATGTGAGGCATGCAGCTGCTCCACGCTGTCAGCACAGGGCTTTCTGACCACTCAGGAGC
5841 TTCCCTCGGCCTTCTGGTAACTGGCATGTCCTGGTCATGTTTGCTGCTGCTTCTGCTTCTGTTTTAATGTGTTCGCTTTC
5921 CATTTGGCAGAGAGACAAGCGAGACACCTCCAACTTCGACAAAGAGTTCACCAGGCAGCCTGTGGAACTGACTCCCACTG
6001 ACAAACTCTTCATCATGAACTTGGACCAAAATGAGTTTGCTGGCTTCTCGTATACTAACCCAGAGTTTGTCATTAATGTG
6081 TAGGTGAATGCAGAGTCCATTGCCGAGCCTGTGTGTAAGGCTTCAGGCCGAATGTCTGTTACCAATTCCAGTCTTCCAGG
6161 ATTCATGGTGCCTCTATTGGCATCCGTCATGTGGAGAGCTTGTCTTAGAGGGCTTTTCTTTGTATGTTTAGCTTGCTAGT
6241 TTGTTTTCTATATTTCAAACTGTTTAGTTTAGAATAAGAGCATTGCCCACCGATAGAGGTACACTTTTCCAGACTTCCAG
6321 AAACTCATCCAATGAACCGACAGTGTCAAAACTTAACTGTGTCCAATACCAAAATGCTTCAGTATTTGTAATTTTTAAAG
6401 TCAGATGCTGATGTTCCTGGTCAAAGTTTTTACAGTTACTCTCTACTATCTCCTTTGAATGCTACCTAAGCATGACCGGT
6481 ATTTTTAAAAGTTGTGAGTAAGCTTTGCAGTTCCTGTGAATTCGTGTCTCTTGGAGGAAACTTTTTGTTTAAGAATTGGT
6561 ATGATTAAACTGAATTCATATATGCAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auaccGUCUGAC-ACUAAACAAc 5'
               :|| :|| | :|||||| 
Target 5' atgttTAGCTTGCTAGTTTGTTt 3'
 6224 - 6246 137.00 -5.30
2 
miRNA  3' auACCGUCUGACACUAAACAac 5'
            ||| :||  | |||||||  
Target 5' caTGG-GGA-GGGGATTTGTct 3'
 994 - 1013 134.00 -12.40
3 
miRNA  3' auaccGUCUGAC---ACUAAACAAc 5'
               || | ||   || |||||| 
Target 5' ttaacCATAATGGCCTGCTTTGTTc 3'
 930 - 954 125.00 -8.60
Experimental Support 1 for Non-Functional miRNA-Target Interaction
miRNA:Target xx
Validation Method
     
Conditions MIN6 cells , HEK293 cells
Disease CCCGTCGACGGGGCAATGCTCT TATTCTAAACTAAACAG; 18751.0
Location of target site 3'UTR
Tools used in this research microarray
Original Description (Extracted from the article) ... "MicroRNA-7a regulates pancreatic 脦虏 cell function we determined that miR-7 is a negative regulator of GSIS in 脦虏 cells. Using Mir7a2 deficient mice "Mutations of miR-7 sites in selected targets conferred complete derepression of luciferase activity demonstrated that direct miRNA engagement on the 3芒鈧虏-UTR of these transcripts was required for gene regulation by miR-7a. Together ...

- Latreille M; Hausser J; Stutzer I; Zhang Q; et al., 2014, The Journal of clinical investigation.

Article - Latreille M; Hausser J; Stutzer I; Zhang Q; et al.
- The Journal of clinical investigation, 2014
Dysfunctional microRNA (miRNA) networks contribute to inappropriate responses following pathological stress and are the underlying cause of several disease conditions. In pancreatic beta cells, miRNAs have been largely unstudied and little is known about how specific miRNAs regulate glucose-stimulated insulin secretion (GSIS) or impact the adaptation of beta cell function to metabolic stress. In this study, we determined that miR-7 is a negative regulator of GSIS in beta cells. Using Mir7a2 deficient mice, we revealed that miR-7a2 regulates beta cell function by directly regulating genes that control late stages of insulin granule fusion with the plasma membrane and ternary SNARE complex activity. Transgenic mice overexpressing miR-7a in beta cells developed diabetes due to impaired insulin secretion and beta cell dedifferentiation. Interestingly, perturbation of miR-7a expression in beta cells did not affect proliferation and apoptosis, indicating that miR-7 is dispensable for the maintenance of endocrine beta cell mass. Furthermore, we found that miR-7a levels are decreased in obese/diabetic mouse models and human islets from obese and moderately diabetic individuals with compensated beta cell function. Our results reveal an interconnecting miR-7 genomic circuit that regulates insulin granule exocytosis in pancreatic beta cells and support a role for miR-7 in the adaptation of pancreatic beta cell function in obesity and type 2 diabetes.
LinkOut: [PMID: 24789908]
7 mmu-miR-7a-1-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT438818 Pfn2 profilin 2 4 1
MIRT438823 Prkcb protein kinase C, beta 4 1
MIRT438845 Snca synuclein, alpha 4 1
MIRT438864 Zdhhc9 zinc finger, DHHC domain containing 9 4 1
MIRT438865 Wipf2 WAS/WASL interacting protein family, member 2 4 1
MIRT438867 Cplx1 complexin 1 4 1
MIRT438876 Basp1 brain abundant, membrane attached signal protein 1 4 1

Error report submission