miRTarBase - #MIRT438867


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pre-miRNA Information
pre-miRNA mmu-mir-7a-1   
Genomic Coordinates chr13: 58392779 - 58392886
Description Mus musculus miR-7a-1 stem-loop
Comment miR-7a (previously named miR-7) was predicted by computational methods using conservation between mouse, human and Fugu rubripes sequences . The ends of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-7a-1-3p
Sequence 66| CAACAAAUCACAGUCUGCCAUA |87
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Cplx1   
Synonyms 921-S
Description complexin 1
Transcript NM_007756   
Expression
Putative miRNA Targets on Cplx1
3'UTR of Cplx1
(miRNA target sites are highlighted)		 
>Cplx1|NM_007756|3'UTR
   1 TGATATTGGGTCAGCACCAGGAGCCCTGCCCACTGTACAGACCCCCGCGGAGTCTCCCCCCAACAAGGGATGTTGGGAGC
  81 AGATGCAGCCCCCCTACCCCCGCAAAAATAAGCCATAGTCCTAGATCTATCCTGCCCATGTCCCCCCTCGCGCCTCGGGA
 161 GCCTCTGGCCACCCCTCCCCATACTGCCATCACTGACCCCACCAGGCAAGGGTGTGACCCTCAGGCCCCTGTGCTGTGCA
 241 TCCTTGCCTTCGGGTGGTTGGACCCCTCCTTACTAATCTTAAGTTCACCATCCCCACAGAGCCTGCCCAAGGTCAGGCCC
 321 CGAGGGCCAGCCCTGACCACCCCTGGATGGGTGGCTACCAGTCAGGGCCTAAACTCAGGAGCACAGCCATAGTGGGGTGG
 401 GACTTGGGGAATGAGCAGTGGGTGCTTGGGGCCCCCAGCCCTTCCCTGAGTCCATTGCCCCAGTTTCTGGACTTGTTGGA
 481 CCTGCTGGACCATTCGCTCTGTCTTCCTGTCTGTGAGCTACCACCCTAGTCTTCCCCAGCAGCCTTTGCATATTTTGTCC
 561 AGCTGTCTCTTTCTCTGTTGCTCTGCAAGGTTTGGCTTAAGAATTCCATGGCCAGAGGAAACACAGCAATAGATTTGGAG
 641 CGGGTGGTCTCAGACACACTTGGAGTCCCTTGACATCGTCTGTAATGCACTTTCCAGAGTCCCATCTTTCCCTGATCCCA
 721 CCTCTCCCTGGTGGCCTCCTGTCCTCTTCATGTGGGCAGGCAGTCTCCTAGGATCTTCCTGGCTTTACCCCTGGACACCT
 801 ACCCTCCAAATGCCCGTCTTCCTAAAAGCCCTGGGTTCTGGTGATTCCCGCCCGTGCAGAGCAGACAGAGGGCCGCATAA
 881 TCAAGTGTCCCACCCTCTCCACTATTCCTCCTCTGAAGGGCTGACAGTGGCCGGTAAATGCAAGCTCCTGGGCTTGGGCT
 961 GTGGTGTTTTGTTCTGTGTGTGGCCCAGGGGCAGTATGACCCAACTACTCCCCCTTTGCCCAGCCAGCAGCCATTGTTCT
1041 TCATATTTGTTTAATTTACATCATAATATGTTGAATCTCAGGTAAATGAGGTCTGTATTTGGTAAGTTTTATCTTGACAG
1121 AAAGGCCAGCCTGGTCTTCCCATAACCCTTCCTGTCCACAGTAAAACTGAATTAAGTGTCCATGAGTTTTGGGCCAGGTG
1201 TGTGGCTTAGCATTGACCTTCATGACCTTACATAGCTCTTTAGAGAAGCCATAACAATTAGACTGCAATACTATTCAGAA
1281 TGCCCTCTGCCCAAAGAGATGAAGCATGCTCGGCCCACAGTACCTTGCTCACCTGGGCCATTCTCTGCGCCTCTGGCTGG
1361 TTTTCATGAATTGAGAAGCCAGCTCTGAGCAGATGGCCCTCTCTTGAGTCAATCCTGCATCCTGTTCCTAGCGCATGGGG
1441 CACCGGGCGCTGAGCTTAGAATAGCCCATGTGTTACCTGTCTGTCTGTCCTCGTGCCTGCACCTCCTGCATGTCAGAGGC
1521 CTCACATTTTCTCTCTGAGGGAATCACAGCCAATAAACAGTGATTTCACACCGTGAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auacCGUC--UGACA------CUAAACAAc 5'
              ||||  |:|||       ||||||| 
Target 5' gccaGCAGCCATTGTTCTTCATATTTGTTt 3'
 1023 - 1052 150.00 -7.10
2 
miRNA  3' auaCCG--UCUGA---CACUAAACAAc 5'
             |||  :|:||   ||| |||||| 
Target 5' ctgGGCTTGGGCTGTGGTGTTTTGTTc 3'
 948 - 974 133.00 -16.90
3 
miRNA  3' auaccGUCUGACA-CUAAACAAc 5'
               ||| :| | || ||||| 
Target 5' tgcccCAGTTTCTGGACTTGTTg 3'
 456 - 478 125.00 -12.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions MIN6 cells , HEK293 cells
Disease CCCCTCGAGCACGGTGTGAAAT CACTGTTTATTG
Location of target site 3'UTR
Tools used in this research microarray
Original Description (Extracted from the article) ... "MicroRNA-7a regulates pancreatic 脦虏 cell function we determined that miR-7 is a negative regulator of GSIS in 脦虏 cells. Using Mir7a2 deficient mice ...

- Latreille M; Hausser J; Stutzer I; Zhang Q; et al., 2014, The Journal of clinical investigation.

Article - Latreille M; Hausser J; Stutzer I; Zhang Q; et al.
- The Journal of clinical investigation, 2014
Dysfunctional microRNA (miRNA) networks contribute to inappropriate responses following pathological stress and are the underlying cause of several disease conditions. In pancreatic beta cells, miRNAs have been largely unstudied and little is known about how specific miRNAs regulate glucose-stimulated insulin secretion (GSIS) or impact the adaptation of beta cell function to metabolic stress. In this study, we determined that miR-7 is a negative regulator of GSIS in beta cells. Using Mir7a2 deficient mice, we revealed that miR-7a2 regulates beta cell function by directly regulating genes that control late stages of insulin granule fusion with the plasma membrane and ternary SNARE complex activity. Transgenic mice overexpressing miR-7a in beta cells developed diabetes due to impaired insulin secretion and beta cell dedifferentiation. Interestingly, perturbation of miR-7a expression in beta cells did not affect proliferation and apoptosis, indicating that miR-7 is dispensable for the maintenance of endocrine beta cell mass. Furthermore, we found that miR-7a levels are decreased in obese/diabetic mouse models and human islets from obese and moderately diabetic individuals with compensated beta cell function. Our results reveal an interconnecting miR-7 genomic circuit that regulates insulin granule exocytosis in pancreatic beta cells and support a role for miR-7 in the adaptation of pancreatic beta cell function in obesity and type 2 diabetes.
LinkOut: [PMID: 24789908]
7 mmu-miR-7a-1-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT438818 Pfn2 profilin 2 4 1
MIRT438823 Prkcb protein kinase C, beta 4 1
MIRT438845 Snca synuclein, alpha 4 1
MIRT438864 Zdhhc9 zinc finger, DHHC domain containing 9 4 1
MIRT438865 Wipf2 WAS/WASL interacting protein family, member 2 4 1
MIRT438867 Cplx1 complexin 1 4 1
MIRT438876 Basp1 brain abundant, membrane attached signal protein 1 4 1

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