pre-miRNA Information | |
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pre-miRNA | hsa-mir-873 |
Genomic Coordinates | chr9: 28888879 - 28888955 |
Synonyms | MIRN873, hsa-mir-873, MIR873 |
Description | Homo sapiens miR-873 stem-loop |
Comment | This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques . |
RNA Secondary Structure | |
Associated Diseases |
Mature miRNA Information | ||||||||||||||||||||||
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Mature miRNA | hsa-miR-873-3p | |||||||||||||||||||||
Sequence | 46| GGAGACUGAUGAGUUCCCGGGA |67 | |||||||||||||||||||||
Evidence | Not_experimental | |||||||||||||||||||||
Experiments | ||||||||||||||||||||||
SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
miRNAs in Extracellular Vesicles |
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Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | FKBP1A | ||||||||||||||||||||
Synonyms | FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE | ||||||||||||||||||||
Description | FK506 binding protein 1A | ||||||||||||||||||||
Transcript | NM_000801 | ||||||||||||||||||||
Other Transcripts | NM_054014 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on FKBP1A | |||||||||||||||||||||
3'UTR of FKBP1A (miRNA target sites are highlighted) |
>FKBP1A|NM_000801|3'UTR 1 CAGGAATGGCCTCCTCCCTTAGCTCCCTGTTCTTGGATCTGCCATGGAGGGATCTGGTGCCTCCAGACATGTGCACATGA 81 ATCCATATGGAGCTTTTCCTGATGTTCCACTCCACTTTGTATAGACATCTGCCCTGACTGAATGTGTTCTGTCACTCAGC 161 TTTGCTTCCGACACCTCTGTTTCCTCTTCCCCTTTCTCCTCGTATGTGTGTTTACCTAAACTATATGCCATAAACCTCAA 241 GTTATTCATTTTATTTTGTTTTCATTTTGGGGTGAAGATTCAGTTTCAGTCTTTTGGATATAGGTTTCCAATTAAGTACA 321 TGGTCAAGTATTAACAGCACAAGTGGTAGGTTAACATTAGAATAGGAATTGGTGTTGGGGGGGGGGTTTGCAAGAATATT 401 TTATTTTAATTTTTTGGATGAAATTTTTATCTATTATATATTAAACATTCTTGCTGCTGCGCTGCAAAGCCATAGCAGAT 481 TTGAGGCGCTGTTGAGGACTGAATTACTCTCCAAGTTGAGAGATGTCTTTGGGTTAAATTAAAAGCCCTACCTAAAACTG 561 AGGTGGGGATGGGGAGAGCCTTTGCCTCCACCATTCCCACCCACCCTCCCCTTAAACCCTCTGCCTTTGAAAGTAGATCA 641 TGTTCACTGCAATGCTGGACACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTTGTGGCCTTTTT 721 TTTTTTTCATCCTGTGGTTTTTCTAATGGACTTTCAGGAATTTTGTAATCTCATAACTTTCCAAGCTCCACCACTTCCTA 801 AATCTTAAGAACTTTAATTGACAGTTTCAATTGAAGGTGCTGTTTGTAGACTTAACACCCAGTGAAAGCCCAGCCATCAT 881 GACAAATCCTTGAATGTTCTCTTAAGAAAATGATGCTGGTCATCGCAGCTTCAGCATCTCCTGTTTTTTGATGCTTGGCT 961 CCCTCTGCTGATCTCAGTTTCCTGGCTTTTCCTCCCTCAGCCCCTTCTCACCCCTTTGCTGTCCTGTGTAGTGATTTGGT 1041 GAGAAATCGTTGCTGCACCCTTCCCCCAGCACCATTTATGAGTCTCAAGTTTTATTATTGCAATAAAAGTGCTTTATGCC 1121 GGCTTTTCTCAGAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | BC-1 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
PAR-CLIP data was present in GSM796037. RNA binding protein: AGO2. Condition:4-Thiouridine
PAR-CLIP data was present in GSM796038. RNA binding protein: AGO2. Condition:4-Thiouridine
... - Gottwein E; Corcoran DL; Mukherjee N; et al., 2011, Cell host & microbe. |
Article |
- Gottwein E; Corcoran DL; Mukherjee N; et al. - Cell host & microbe, 2011
Primary effusion lymphoma (PEL) is caused by Kaposi's sarcoma-associated herpesvirus (KSHV) and frequently also harbors Epstein-Barr virus (EBV). The expression of KSHV- and EBV-encoded microRNAs (miRNAs) in PELs suggests a role for these miRNAs in latency and lymphomagenesis. Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites. In addition to a known viral analog of cellular miR-155, we show that KSHV encodes a viral miRNA that mimics cellular miR-142-3p function. In summary, this study identifies an extensive list of KSHV miRNA targets, which are likely to influence viral replication and pathogenesis.
LinkOut: [PMID: 22100165]
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Experimental Support 2 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | LCL35 |
Disease | MIMAT0022717 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
"PAR-CLIP data was present in GSM1020022. RNA binding protein: AGO2. Condition:EBV B95-8-infected
... - Skalsky RL; Corcoran DL; Gottwein E; Frank et al., 2012, PLoS pathogens. |
Article |
- Skalsky RL; Corcoran DL; Gottwein E; Frank et al. - PLoS pathogens, 2012
Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus linked to a number of B cell cancers and lymphoproliferative disorders. During latent infection, EBV expresses 25 viral pre-microRNAs (miRNAs) and induces the expression of specific host miRNAs, such as miR-155 and miR-21, which potentially play a role in viral oncogenesis. To date, only a limited number of EBV miRNA targets have been identified; thus, the role of EBV miRNAs in viral pathogenesis and/or lymphomagenesis is not well defined. Here, we used photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) combined with deep sequencing and computational analysis to comprehensively examine the viral and cellular miRNA targetome in EBV strain B95-8-infected lymphoblastoid cell lines (LCLs). We identified 7,827 miRNA-interaction sites in 3,492 cellular 3'UTRs. 531 of these sites contained seed matches to viral miRNAs. 24 PAR-CLIP-identified miRNA:3'UTR interactions were confirmed by reporter assays. Our results reveal that EBV miRNAs predominantly target cellular transcripts during latent infection, thereby manipulating the host environment. Furthermore, targets of EBV miRNAs are involved in multiple cellular processes that are directly relevant to viral infection, including innate immunity, cell survival, and cell proliferation. Finally, we present evidence that myc-regulated host miRNAs from the miR-17/92 cluster can regulate latent viral gene expression. This comprehensive survey of the miRNA targetome in EBV-infected B cells represents a key step towards defining the functions of EBV-encoded miRNAs, and potentially, identifying novel therapeutic targets for EBV-associated malignancies.
LinkOut: [PMID: 22291592]
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CLIP-seq Support 1 for dataset GSM1020022 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | LCL35 / EBV B95-8-infected, 4-thiouridine, RNase T1 |
Location of target site | ENST00000400137.4 | 3UTR | UCAAGUUUUAUUAUUGCAAUAAAAG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 22291592 / GSE41437 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM796037 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | BC-1 / 4-Thiouridine |
Location of target site | ENST00000400137.4 | 3UTR | UCAAGUUUUAUUAUUGCAAUAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 22100165 / GSE32109 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset GSM796038 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | BC-1 / 4-Thiouridine |
Location of target site | ENST00000400137.4 | 3UTR | UCAAGUUUUAUUAUUGCAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 22100165 / GSE32109 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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108 hsa-miR-873-3p Target Genes:
Functional analysis:
ID | Target | Description | Validation methods | |||||||||
Strong evidence | Less strong evidence | |||||||||||
MIRT081178 | MIDN | midnolin | 2 | 4 | ||||||||
MIRT109809 | ZFX | zinc finger protein, X-linked | 2 | 4 | ||||||||
MIRT242383 | TMC5 | transmembrane channel like 5 | 2 | 4 | ||||||||
MIRT444003 | METRN | meteorin, glial cell differentiation regulator | 2 | 4 | ||||||||
MIRT444097 | SEPHS1 | selenophosphate synthetase 1 | 2 | 2 | ||||||||
MIRT446158 | RPL12 | ribosomal protein L12 | 2 | 2 | ||||||||
MIRT446859 | SAMD9L | sterile alpha motif domain containing 9 like | 2 | 2 | ||||||||
MIRT447275 | FZD5 | frizzled class receptor 5 | 2 | 2 | ||||||||
MIRT447391 | TMPRSS15 | transmembrane protease, serine 15 | 2 | 2 | ||||||||
MIRT448817 | FKBP1A | FK506 binding protein 1A | 2 | 4 | ||||||||
MIRT450582 | HIST1H2BG | histone cluster 1 H2B family member g | 2 | 2 | ||||||||
MIRT451776 | USP36 | ubiquitin specific peptidase 36 | 2 | 2 | ||||||||
MIRT457961 | ABCC5 | ATP binding cassette subfamily C member 5 | 2 | 4 | ||||||||
MIRT458424 | KLHL38 | kelch like family member 38 | 2 | 4 | ||||||||
MIRT461383 | SLFN12L | schlafen family member 12 like | 2 | 2 | ||||||||
MIRT467793 | SLC2A14 | solute carrier family 2 member 14 | 2 | 2 | ||||||||
MIRT476517 | GABRB1 | gamma-aminobutyric acid type A receptor beta1 subunit | 2 | 2 | ||||||||
MIRT480290 | C7orf73 | short transmembrane mitochondrial protein 1 | 2 | 4 | ||||||||
MIRT482745 | HES7 | hes family bHLH transcription factor 7 | 2 | 10 | ||||||||
MIRT483189 | HIST1H2AH | histone cluster 1 H2A family member h | 2 | 6 | ||||||||
MIRT486545 | DCTN4 | dynactin subunit 4 | 2 | 2 | ||||||||
MIRT486581 | ZNF619 | zinc finger protein 619 | 2 | 2 | ||||||||
MIRT492604 | POLR3E | RNA polymerase III subunit E | 2 | 2 | ||||||||
MIRT494130 | DCAF7 | DDB1 and CUL4 associated factor 7 | 2 | 6 | ||||||||
MIRT496023 | ZBED3 | zinc finger BED-type containing 3 | 2 | 2 | ||||||||
MIRT497121 | NBEAL1 | neurobeachin like 1 | 2 | 2 | ||||||||
MIRT497400 | TMEM245 | transmembrane protein 245 | 2 | 2 | ||||||||
MIRT501410 | RANBP10 | RAN binding protein 10 | 2 | 2 | ||||||||
MIRT510947 | PPTC7 | PTC7 protein phosphatase homolog | 2 | 6 | ||||||||
MIRT512494 | ARID2 | AT-rich interaction domain 2 | 2 | 2 | ||||||||
MIRT512595 | ZNF783 | zinc finger family member 783 | 2 | 2 | ||||||||
MIRT512612 | CNTN4 | contactin 4 | 2 | 2 | ||||||||
MIRT517808 | UGDH | UDP-glucose 6-dehydrogenase | 2 | 6 | ||||||||
MIRT520686 | TMED7 | transmembrane p24 trafficking protein 7 | 2 | 4 | ||||||||
MIRT526179 | HEPH | hephaestin | 2 | 2 | ||||||||
MIRT532568 | CSTF1 | cleavage stimulation factor subunit 1 | 2 | 2 | ||||||||
MIRT533979 | TADA2A | transcriptional adaptor 2A | 2 | 2 | ||||||||
MIRT538622 | CCSER2 | coiled-coil serine rich protein 2 | 2 | 4 | ||||||||
MIRT539703 | EIF3H | eukaryotic translation initiation factor 3 subunit H | 2 | 2 | ||||||||
MIRT539806 | GAPVD1 | GTPase activating protein and VPS9 domains 1 | 2 | 2 | ||||||||
MIRT540422 | FAM83F | family with sequence similarity 83 member F | 2 | 2 | ||||||||
MIRT540506 | CXCL10 | C-X-C motif chemokine ligand 10 | 2 | 2 | ||||||||
MIRT540619 | F2RL2 | coagulation factor II thrombin receptor like 2 | 2 | 2 | ||||||||
MIRT542423 | ZNF331 | zinc finger protein 331 | 2 | 2 | ||||||||
MIRT542454 | AKR7A2 | aldo-keto reductase family 7 member A2 | 2 | 2 | ||||||||
MIRT543383 | CC2D2A | coiled-coil and C2 domain containing 2A | 2 | 2 | ||||||||
MIRT544777 | CSTF2T | cleavage stimulation factor subunit 2 tau variant | 2 | 4 | ||||||||
MIRT544923 | ERCC4 | ERCC excision repair 4, endonuclease catalytic subunit | 2 | 2 | ||||||||
MIRT549768 | ZNF611 | zinc finger protein 611 | 2 | 4 | ||||||||
MIRT551242 | COLEC10 | collectin subfamily member 10 | 2 | 2 | ||||||||
MIRT560474 | ENSA | endosulfine alpha | 2 | 2 | ||||||||
MIRT569738 | GPR173 | G protein-coupled receptor 173 | 2 | 2 | ||||||||
MIRT571297 | CHCHD4 | coiled-coil-helix-coiled-coil-helix domain containing 4 | 2 | 2 | ||||||||
MIRT572345 | CKAP2L | cytoskeleton associated protein 2 like | 2 | 2 | ||||||||
MIRT573112 | ERBB2IP | erbb2 interacting protein | 2 | 2 | ||||||||
MIRT607744 | ANGPT4 | angiopoietin 4 | 2 | 2 | ||||||||
MIRT607903 | SPRYD4 | SPRY domain containing 4 | 2 | 2 | ||||||||
MIRT611744 | SERPING1 | serpin family G member 1 | 2 | 4 | ||||||||
MIRT615101 | BNC2 | basonuclin 2 | 2 | 2 | ||||||||
MIRT619124 | CD40LG | CD40 ligand | 2 | 2 | ||||||||
MIRT625572 | ANKRD42 | ankyrin repeat domain 42 | 2 | 2 | ||||||||
MIRT629038 | KLLN | killin, p53-regulated DNA replication inhibitor | 2 | 2 | ||||||||
MIRT633986 | SLC35E2 | solute carrier family 35 member E2 | 2 | 2 | ||||||||
MIRT635675 | COX18 | COX18, cytochrome c oxidase assembly factor | 2 | 4 | ||||||||
MIRT637471 | DEFB105B | defensin beta 105B | 2 | 4 | ||||||||
MIRT637503 | DEFB105A | defensin beta 105A | 2 | 4 | ||||||||
MIRT639735 | MAP2K2 | mitogen-activated protein kinase kinase 2 | 2 | 2 | ||||||||
MIRT640730 | C9orf64 | chromosome 9 open reading frame 64 | 2 | 2 | ||||||||
MIRT645364 | C9orf47 | chromosome 9 open reading frame 47 | 2 | 2 | ||||||||
MIRT647938 | RNF152 | ring finger protein 152 | 2 | 2 | ||||||||
MIRT649400 | SH2D4A | SH2 domain containing 4A | 2 | 2 | ||||||||
MIRT656860 | KIN | Kin17 DNA and RNA binding protein | 2 | 2 | ||||||||
MIRT663470 | POFUT2 | protein O-fucosyltransferase 2 | 2 | 2 | ||||||||
MIRT663507 | NKAPL | NFKB activating protein like | 2 | 4 | ||||||||
MIRT667690 | KNSTRN | kinetochore localized astrin/SPAG5 binding protein | 2 | 2 | ||||||||
MIRT677403 | PCNP | PEST proteolytic signal containing nuclear protein | 2 | 2 | ||||||||
MIRT678682 | SCUBE3 | signal peptide, CUB domain and EGF like domain containing 3 | 2 | 2 | ||||||||
MIRT678789 | NUPL2 | nucleoporin like 2 | 2 | 2 | ||||||||
MIRT680649 | KIAA1456 | KIAA1456 | 2 | 2 | ||||||||
MIRT682450 | MTX3 | metaxin 3 | 2 | 2 | ||||||||
MIRT682740 | CA6 | carbonic anhydrase 6 | 2 | 2 | ||||||||
MIRT684421 | TUFT1 | tuftelin 1 | 2 | 2 | ||||||||
MIRT690535 | TRAPPC2 | trafficking protein particle complex 2 | 2 | 2 | ||||||||
MIRT690595 | C17orf105 | chromosome 17 open reading frame 105 | 2 | 2 | ||||||||
MIRT690771 | PLA2G2C | phospholipase A2 group IIC | 2 | 2 | ||||||||
MIRT692233 | ALDH1B1 | aldehyde dehydrogenase 1 family member B1 | 2 | 2 | ||||||||
MIRT693667 | MXRA7 | matrix remodeling associated 7 | 2 | 2 | ||||||||
MIRT695551 | CLPB | ClpB homolog, mitochondrial AAA ATPase chaperonin | 2 | 2 | ||||||||
MIRT695870 | C19orf52 | translocase of inner mitochondrial membrane 29 | 2 | 2 | ||||||||
MIRT696214 | LYZ | lysozyme | 2 | 2 | ||||||||
MIRT698368 | TMED4 | transmembrane p24 trafficking protein 4 | 2 | 2 | ||||||||
MIRT700795 | PHTF2 | putative homeodomain transcription factor 2 | 2 | 2 | ||||||||
MIRT701640 | MYLK3 | myosin light chain kinase 3 | 2 | 2 | ||||||||
MIRT702989 | HERPUD2 | HERPUD family member 2 | 2 | 2 | ||||||||
MIRT703627 | FBXL3 | F-box and leucine rich repeat protein 3 | 2 | 2 | ||||||||
MIRT703783 | FAM102B | family with sequence similarity 102 member B | 2 | 2 | ||||||||
MIRT704293 | DDX19B | DEAD-box helicase 19B | 2 | 2 | ||||||||
MIRT704828 | CDC73 | cell division cycle 73 | 2 | 2 | ||||||||
MIRT705014 | CAMK2N1 | calcium/calmodulin dependent protein kinase II inhibitor 1 | 2 | 2 | ||||||||
MIRT708480 | OLR1 | oxidized low density lipoprotein receptor 1 | 2 | 2 | ||||||||
MIRT710770 | PHF7 | PHD finger protein 7 | 2 | 2 | ||||||||
MIRT718918 | TRIM66 | tripartite motif containing 66 | 2 | 2 | ||||||||
MIRT720390 | ZNF549 | zinc finger protein 549 | 2 | 2 | ||||||||
MIRT720593 | TTC39C | tetratricopeptide repeat domain 39C | 2 | 2 | ||||||||
MIRT723515 | SIGLEC8 | sialic acid binding Ig like lectin 8 | 2 | 2 | ||||||||
MIRT724458 | PRKX | protein kinase, X-linked | 2 | 2 | ||||||||
MIRT737267 | UMAD1 | UBAP1-MVB12-associated (UMA) domain containing 1 | 3 | 0 | ||||||||
MIRT737356 | ZIC2 | Zic family member 2 | 4 | 0 |
miRNA-Drug Associations | ||||||||||||||||||
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miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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