miRTarBase - #MIRT458521


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pre-miRNA Information
pre-miRNA hsa-mir-383   
Genomic Coordinates chr8: 14853438 - 14853510
Synonyms MIRN383, hsa-mir-383, MIR383
Description Homo sapiens miR-383 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-383-5p
Sequence 7| AGAUCAGAAGGUGAUUGUGGCU |28
Evidence Experimental
Experiments Cloned
DRVs in miRNA
Mutant ID Mutant Position Mutant Source
COSN26994512 7 COSMIC
COSN30109318 22 COSMIC
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs112302475 1 dbSNP
rs756858316 1 dbSNP
rs757040124 3 dbSNP
rs1419085595 4 dbSNP
rs912771170 5 dbSNP
rs1190491141 6 dbSNP
rs986831934 7 dbSNP
rs1471946963 9 dbSNP
rs1237080909 10 dbSNP
rs757526216 11 dbSNP
rs1431645579 13 dbSNP
rs763747200 13 dbSNP
rs184836993 15 dbSNP
rs913343525 17 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol C5orf22   
Synonyms -
Description chromosome 5 open reading frame 22
Transcript NM_018356   
Expression
Putative miRNA Targets on C5orf22
3'UTR of C5orf22
(miRNA target sites are highlighted)		 
>C5orf22|NM_018356|3'UTR
   1 AACAAACAAAACATTAGGCTCCTGTTGTATCTTGGTTTAGTAACAGGCCCTTAATTAACTTATTTGTACATGAGTCTTCC
  81 AGAGAACACTGTTTTATATTAACTTTCAGTTGAAATCTTTCAGATATTTTGAATCTCTGAACAACCATTGTCAGTTGTGA
 161 ATGATGGTAAATTTTTTGGCATCAAGTCTCATAACCCCAACTGATAGAACTGTTGCTTATCTGTCTTCCTTAAGTATTTT
 241 TTAGGGTTTTGTTTTTTTTTTTGTTTGTTTGTTTGTTTGTCTTCACTTTTCCCCCAGGTCTGTTGAGCTGTATGAGATTC
 321 ATTCATACTTCATTTATTCATTCAACTAATATTTGTTGAACACTTACATGTACCAGACATTATTAAGTGCTGGGTATATG
 401 GTAATGAACAGAATAGACAAGGCCCCTGCCCTTTTAGGGGAGACAGATGAGAAGTAAATTACGGGTTATGAGAAATGTTA
 481 TGAAGGAAAGGACAACAACAGACATGTCTTAGTACTTAGGGTATCATGGCTTTATAGGAAAGTAACATTCTCTATCTTTT
 561 TTTTTTTTTTTAATTTTATAAAAATAGAGATGGGGTCTCACCATGTTACCCAGGCTGGTCTCAAACTCCTGGGCACAAGC
 641 AATCCACCTGCCTTCGCCTCCCGAAGTGCTGGGATTACAGATGTAAGTCACTACCCCAGCCAGAAACTAACATTCTCAAA
 721 AGCTACTACCAAAAAAACCCCACAAATTTGCCAATCCATGAAAGTATCATAGGAAGTCTACCTTTCCAAGGAATTTGGCA
 801 TTGTGGCCCTGCTCTTGCCAAATGCTCTCCAGCATTGTTGGTATCAAGGTGGTCTGGTTGAGTAAACTTGACCAAACAAA
 881 AATTAACTGTTTTTTGCTTTTCTGTTTTTGTTTTGCTGATCAAAAAAGCCCTTCAGGGTCTCTTGTGCATCCAGGAGGTC
 961 ATTGTAGATATCAATAATATAGTGCCTAAGTGCCACTTTATTGCCAAGTCTAGATTGATACTTTTTTCAGATAAACCAGC
1041 TTTTTATGTAAAGAGTAAGGGAAAAAGTTAAATCTTTAATTCTGACCTGCCATAAATACCCAAAGATATAAACTGTCTTC
1121 CACCACCCCCCTCATAACTAAGACATCCTTCCTGAGTCACTCTTAATCATGAAACTTGATTTTCTCAATTGGCCAGTCTT
1201 CTGATCTTTAGTATCTCTTTAGTTCAGTAATTTTTACCTACCTACTTGATTTATTTTCCTTTAAAAGGTGAAATGACATT
1281 TAAAGAAAAACAAACACCCATCATTCCTCAGTCCCAACACAGCAGTTCTTTTCACTCTTGCTTGTAACTTTCAGGCCTTA
1361 CCCACATGTGTTCCTATAGAGTTGCATGTAGCTAAAATTATAGTGTGCTGCCTAACTTTGTGCTAATAACGAAGGACACA
1441 CATTATCATTGCCTCACAGTGGATGGCACCTGACCCCATTTTTCCAAGGATTCTATTTTCCAGTGAATTTTTGTCACCGA
1521 GTTAATTTCCCAAGTCTGATAGCATGAATGCTTATTTGTGCTGTGAAAAGCAGAGCAGCCTGACTCAGCTTTCCATTTTC
1601 ATAGGATAATATGTGCCAAAAAAGGTTTATTTTCTTGGTAAAATCAGAATTGTAGAATTGTGCTATTTTTAATTTTTAAA
1681 ATTTTTGGTTAGAGCTTATAGACATTACTTTTATGATTATTTTGCGTGCTAAATAAGTTAAAGGGTATGTTTTGAACTAT
1761 AGCACTAATTAATGTGTGTGATCCTAGTATGTTGATAGCTTTGATTTTGTGAGTGGTTTACTAGTCATTTATTATGATTC
1841 CCATGAACTCTGATATGATTCATTGTGGTTTTAACTCAGGTTGAATAAAAGCATCCATTTCTTTTATAGGAAGGTTTGTT
1921 TAATTCTCATGTTCTGTGATCATCTCTTTGAGAATAAAATTCAGTCCCTAATAGTTGAAAAGAATCAACTTAGTAAATTA
2001 TGTCTTGCACATTCCAAATAGGAAGGTATTTACTTTATCAAGATGTGTTTCATTTGTGTTCTGAGTACCAGTGAGGGGAT
2081 ACCGTGGTACAGTGTTTTGAATTGTGTACTAATATCATTAAAGCATGGGATTTTAGTGGCTTTTTAAAAAATGTGAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ucGGUGUUA----GU-GGAAGACUAGa 5'
            :| ||||    || :||||||||| 
Target 5' ttTCTCAATTGGCCAGTCTTCTGATCt 3'
 1181 - 1207 162.00 -18.50
2 
miRNA  3' ucGGUGUUAGUGGAAGACUAga 5'
            |||::|  ||  ||||||  
Target 5' tcCCATGA--AC--TCTGATat 3'
 1839 - 1856 132.00 -8.30
3 
miRNA  3' ucgguGUUAGUGG---AAGACUAga 5'
               :|||: ||    ||||||  
Target 5' cgagtTAATTTCCCAAGTCTGATag 3'
 1518 - 1542 129.00 -11.60
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30494362 11 COSMIC
COSN31506445 14 COSMIC
COSN31598767 23 COSMIC
COSN30515931 25 COSMIC
COSN30146981 33 COSMIC
COSN507303 35 COSMIC
COSN30129116 64 COSMIC
COSN31493625 73 COSMIC
COSN18729926 82 COSMIC
COSN31495913 110 COSMIC
COSN31830839 116 COSMIC
COSN30163868 118 COSMIC
COSN31605391 118 COSMIC
COSN30527655 180 COSMIC
COSN9949508 216 COSMIC
COSN29723446 256 COSMIC
COSN28275772 264 COSMIC
COSN24270754 306 COSMIC
COSN29449840 828 COSMIC
COSN9432527 842 COSMIC
COSN27648929 975 COSMIC
COSN26759587 1278 COSMIC
COSN2102349 1309 COSMIC
COSN5603747 1491 COSMIC
COSN32063670 1519 COSMIC
COSN32108839 1547 COSMIC
COSN32108840 1746 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1370419139 4 dbSNP
rs1010944323 18 dbSNP
rs1192026831 21 dbSNP
rs377752655 23 dbSNP
rs768271912 24 dbSNP
rs776327008 26 dbSNP
rs1268821742 35 dbSNP
rs761227162 35 dbSNP
rs769284735 37 dbSNP
rs1274682074 40 dbSNP
rs201440242 46 dbSNP
rs551288912 48 dbSNP
rs768170059 49 dbSNP
rs1275292915 55 dbSNP
rs571072908 65 dbSNP
rs1337210779 67 dbSNP
rs1030285668 68 dbSNP
rs540108237 71 dbSNP
rs1234223729 75 dbSNP
rs1333098662 95 dbSNP
rs1301340360 97 dbSNP
rs1371654573 98 dbSNP
rs1310399739 118 dbSNP
rs779939726 121 dbSNP
rs1419244097 133 dbSNP
rs1183739124 136 dbSNP
rs1173585950 138 dbSNP
rs1414776098 142 dbSNP
rs1050759798 146 dbSNP
rs547218099 149 dbSNP
rs1431027434 151 dbSNP
rs1196588790 161 dbSNP
rs886780621 164 dbSNP
rs1207207714 172 dbSNP
rs567030030 172 dbSNP
rs1474531071 185 dbSNP
rs909544995 186 dbSNP
rs1219633436 191 dbSNP
rs1391881872 192 dbSNP
rs1407495950 212 dbSNP
rs534750622 213 dbSNP
rs1281635143 220 dbSNP
rs1222914915 225 dbSNP
rs1327019663 225 dbSNP
rs1290361843 231 dbSNP
rs1440627134 237 dbSNP
rs12521382 243 dbSNP
rs12513495 244 dbSNP
rs1354517898 250 dbSNP
rs1335238689 251 dbSNP
rs1017106467 252 dbSNP
rs1173820902 252 dbSNP
rs558847315 252 dbSNP
rs1013524117 253 dbSNP
rs535047024 253 dbSNP
rs568076291 254 dbSNP
rs199736333 256 dbSNP
rs915759205 256 dbSNP
rs71584569 257 dbSNP
rs1203264028 259 dbSNP
rs11748072 260 dbSNP
rs1373190900 260 dbSNP
rs924567188 260 dbSNP
rs924490551 261 dbSNP
rs1450553596 262 dbSNP
rs200855500 263 dbSNP
rs990228126 263 dbSNP
rs1468949438 264 dbSNP
rs368305221 264 dbSNP
rs59748311 264 dbSNP
rs914666621 264 dbSNP
rs577088381 265 dbSNP
rs545649737 267 dbSNP
rs1358193235 268 dbSNP
rs1193865054 272 dbSNP
rs552787303 274 dbSNP
rs1271784836 276 dbSNP
rs1483194240 280 dbSNP
rs1206636838 282 dbSNP
rs768225738 291 dbSNP
rs893791125 291 dbSNP
rs572695168 294 dbSNP
rs35364739 297 dbSNP
rs1285783541 299 dbSNP
rs541945348 303 dbSNP
rs921179979 313 dbSNP
rs1182807888 321 dbSNP
rs1465487566 326 dbSNP
rs933873051 338 dbSNP
rs1485087310 341 dbSNP
rs548561296 348 dbSNP
rs1290764353 351 dbSNP
rs1230515975 358 dbSNP
rs1357990511 361 dbSNP
rs886744675 373 dbSNP
rs1436323131 379 dbSNP
rs1365416027 387 dbSNP
rs866113634 402 dbSNP
rs1369615489 407 dbSNP
rs562024290 412 dbSNP
rs1427952816 424 dbSNP
rs1344084744 427 dbSNP
rs1029820515 429 dbSNP
rs1473944875 432 dbSNP
rs1166539415 436 dbSNP
rs1426447383 441 dbSNP
rs887396388 446 dbSNP
rs1389738575 447 dbSNP
rs1162653794 448 dbSNP
rs1038103415 449 dbSNP
rs898266491 454 dbSNP
rs753698335 456 dbSNP
rs1184994962 458 dbSNP
rs1373692579 463 dbSNP
rs191747277 464 dbSNP
rs1016638986 470 dbSNP
rs1224872039 471 dbSNP
rs1334664850 478 dbSNP
rs1023177781 486 dbSNP
rs1229528577 492 dbSNP
rs1309632804 495 dbSNP
rs80136932 497 dbSNP
rs1003098246 500 dbSNP
rs1031947121 504 dbSNP
rs1293570386 508 dbSNP
rs1406275437 509 dbSNP
rs1269413350 529 dbSNP
rs1342661022 530 dbSNP
rs991273640 535 dbSNP
rs544301657 547 dbSNP
rs914656368 548 dbSNP
rs1428111461 549 dbSNP
rs1201628329 551 dbSNP
rs1426180517 551 dbSNP
rs1489885292 552 dbSNP
rs1249955557 557 dbSNP
rs1268711874 557 dbSNP
rs1481942241 557 dbSNP
rs576940354 557 dbSNP
rs1204317737 562 dbSNP
rs564458568 572 dbSNP
rs974859434 573 dbSNP
rs1343090969 574 dbSNP
rs971264564 577 dbSNP
rs1200545771 579 dbSNP
rs112109049 589 dbSNP
rs921064128 590 dbSNP
rs1398594550 593 dbSNP
rs1296197246 594 dbSNP
rs1257418670 595 dbSNP
rs981717313 604 dbSNP
rs1050943557 606 dbSNP
rs1479620416 609 dbSNP
rs533677045 622 dbSNP
rs908488211 630 dbSNP
rs1183051895 631 dbSNP
rs924447036 633 dbSNP
rs934603921 635 dbSNP
rs1176936555 637 dbSNP
rs1483791591 643 dbSNP
rs990015889 645 dbSNP
rs1450541667 649 dbSNP
rs915257805 649 dbSNP
rs1170709618 653 dbSNP
rs1038476831 656 dbSNP
rs770062787 657 dbSNP
rs1304797736 663 dbSNP
rs547305363 664 dbSNP
rs1383246549 672 dbSNP
rs1039795593 674 dbSNP
rs899948423 683 dbSNP
rs1468414344 687 dbSNP
rs1044177681 690 dbSNP
rs1159953754 692 dbSNP
rs1455433383 694 dbSNP
rs1362508397 696 dbSNP
rs1181726344 698 dbSNP
rs1424957837 713 dbSNP
rs1404220274 720 dbSNP
rs1380431299 724 dbSNP
rs1182173003 728 dbSNP
rs777988949 730 dbSNP
rs1459668089 731 dbSNP
rs567117531 732 dbSNP
rs1051724799 733 dbSNP
rs887259304 734 dbSNP
rs1003067096 739 dbSNP
rs1258500912 741 dbSNP
rs568503942 743 dbSNP
rs1017174378 744 dbSNP
rs898166289 751 dbSNP
rs548302292 754 dbSNP
rs1012962286 759 dbSNP
rs1319244965 765 dbSNP
rs1383929563 774 dbSNP
rs568081069 781 dbSNP
rs1011575812 790 dbSNP
rs537123863 796 dbSNP
rs1387667054 808 dbSNP
rs1347980759 810 dbSNP
rs1022622511 813 dbSNP
rs971595318 818 dbSNP
rs1193445139 823 dbSNP
rs1384146494 823 dbSNP
rs981198114 835 dbSNP
rs1259034976 839 dbSNP
rs1268120080 843 dbSNP
rs1465574545 849 dbSNP
rs1204082800 871 dbSNP
rs1490482551 888 dbSNP
rs1031496791 895 dbSNP
rs955846322 898 dbSNP
rs1190926647 905 dbSNP
rs1243427269 905 dbSNP
rs1358471732 908 dbSNP
rs1270619910 939 dbSNP
rs533762551 942 dbSNP
rs7711393 943 dbSNP
rs1317676286 944 dbSNP
rs914453701 956 dbSNP
rs12658729 960 dbSNP
rs1379423220 963 dbSNP
rs986623103 965 dbSNP
rs975857004 968 dbSNP
rs1170059834 981 dbSNP
rs1476174281 987 dbSNP
rs539393463 990 dbSNP
rs921256458 992 dbSNP
rs767745599 997 dbSNP
rs934096885 998 dbSNP
rs908446412 1000 dbSNP
rs1389296359 1009 dbSNP
rs1490182919 1011 dbSNP
rs1458571497 1016 dbSNP
rs752443112 1020 dbSNP
rs1366693367 1021 dbSNP
rs35621481 1022 dbSNP
rs1386967879 1024 dbSNP
rs1303024568 1025 dbSNP
rs1051158791 1048 dbSNP
rs1160761727 1049 dbSNP
rs1227247372 1051 dbSNP
rs908728546 1073 dbSNP
rs879637445 1074 dbSNP
rs966019539 1086 dbSNP
rs1281513584 1087 dbSNP
rs1404849821 1090 dbSNP
rs552824228 1092 dbSNP
rs1355671315 1098 dbSNP
rs940132269 1102 dbSNP
rs1210930781 1103 dbSNP
rs948431825 1108 dbSNP
rs572733799 1110 dbSNP
rs1470057653 1114 dbSNP
rs898784086 1116 dbSNP
rs906981532 1117 dbSNP
rs1477633846 1126 dbSNP
rs938825868 1128 dbSNP
rs1183645125 1129 dbSNP
rs1053321788 1130 dbSNP
rs1272108460 1131 dbSNP
rs1204527712 1151 dbSNP
rs1013166027 1166 dbSNP
rs1044479491 1167 dbSNP
rs1488389341 1173 dbSNP
rs1261305751 1190 dbSNP
rs906856846 1191 dbSNP
rs576996875 1192 dbSNP
rs1003051666 1193 dbSNP
rs1223021973 1202 dbSNP
rs1210648100 1211 dbSNP
rs1281797303 1211 dbSNP
rs1440387931 1214 dbSNP
rs1335544482 1231 dbSNP
rs1269820730 1242 dbSNP
rs1337868478 1247 dbSNP
rs1031361200 1252 dbSNP
rs1452888464 1253 dbSNP
rs1407881470 1260 dbSNP
rs955878821 1277 dbSNP
rs1181570634 1288 dbSNP
rs1408536997 1288 dbSNP
rs1011396757 1297 dbSNP
rs185019260 1307 dbSNP
rs1184010454 1309 dbSNP
rs1012211066 1312 dbSNP
rs3733715 1325 dbSNP
rs1241560383 1326 dbSNP
rs965008607 1339 dbSNP
rs1203056041 1341 dbSNP
rs1317601452 1348 dbSNP
rs1286514628 1349 dbSNP
rs900476070 1351 dbSNP
rs575651719 1355 dbSNP
rs1350651036 1356 dbSNP
rs544652473 1362 dbSNP
rs564363777 1363 dbSNP
rs1294850180 1367 dbSNP
rs921275944 1377 dbSNP
rs955378591 1378 dbSNP
rs1393892407 1382 dbSNP
rs189897483 1393 dbSNP
rs540957092 1402 dbSNP
rs908594752 1416 dbSNP
rs1351844719 1417 dbSNP
rs560752174 1420 dbSNP
rs768370640 1421 dbSNP
rs529578948 1431 dbSNP
rs16901281 1432 dbSNP
rs761440143 1435 dbSNP
rs920088579 1436 dbSNP
rs1479797631 1437 dbSNP
rs777333506 1438 dbSNP
rs549335190 1442 dbSNP
rs1015805054 1443 dbSNP
rs949017949 1448 dbSNP
rs1056490 1457 dbSNP
rs530532765 1467 dbSNP
rs1357734288 1491 dbSNP
rs1185700485 1495 dbSNP
rs1227360148 1514 dbSNP
rs573194340 1519 dbSNP
rs182061781 1520 dbSNP
rs1424114690 1527 dbSNP
rs1052709706 1535 dbSNP
rs891584294 1536 dbSNP
rs919877090 1545 dbSNP
rs150819693 1547 dbSNP
rs762978246 1559 dbSNP
rs1426549547 1564 dbSNP
rs1021441402 1574 dbSNP
rs964547621 1575 dbSNP
rs766174210 1580 dbSNP
rs1056491 1585 dbSNP
rs1184797593 1595 dbSNP
rs1308314058 1602 dbSNP
rs1371860939 1604 dbSNP
rs938370889 1606 dbSNP
rs1269773795 1607 dbSNP
rs1052861100 1608 dbSNP
rs1490016239 1609 dbSNP
rs1301535901 1611 dbSNP
rs1379101940 1612 dbSNP
rs1265190328 1613 dbSNP
rs1396927986 1618 dbSNP
rs1307673011 1619 dbSNP
rs1297299461 1620 dbSNP
rs955564869 1623 dbSNP
rs1339272783 1624 dbSNP
rs1283434699 1628 dbSNP
rs1406675277 1629 dbSNP
rs947596035 1634 dbSNP
rs1043686209 1635 dbSNP
rs1340544645 1636 dbSNP
rs986834283 1637 dbSNP
rs1015601176 1639 dbSNP
rs1356429864 1640 dbSNP
rs1462316767 1640 dbSNP
rs961479153 1644 dbSNP
rs1465352658 1646 dbSNP
rs1056492 1649 dbSNP
rs1051755234 1656 dbSNP
rs1344322278 1661 dbSNP
rs1479718581 1662 dbSNP
rs1267293877 1673 dbSNP
rs566449727 1687 dbSNP
rs549566161 1688 dbSNP
rs139267435 1689 dbSNP
rs1483556122 1705 dbSNP
rs575248419 1724 dbSNP
rs867217729 1726 dbSNP
rs555280853 1727 dbSNP
rs928935011 1729 dbSNP
rs144384906 1734 dbSNP
rs376790656 1734 dbSNP
rs1296283697 1735 dbSNP
rs1446280970 1746 dbSNP
rs1168834761 1748 dbSNP
rs1391637564 1755 dbSNP
rs1027308323 1757 dbSNP
rs4867350 1760 dbSNP
rs980016572 1763 dbSNP
rs928249384 1764 dbSNP
rs538001738 1766 dbSNP
rs1052740517 1775 dbSNP
rs748456364 1806 dbSNP
rs1248834613 1810 dbSNP
rs891446742 1824 dbSNP
rs770134045 1833 dbSNP
rs1174772797 1844 dbSNP
rs756385283 1856 dbSNP
rs1457011293 1863 dbSNP
rs988940553 1863 dbSNP
rs773499987 1869 dbSNP
rs1466984288 1881 dbSNP
rs558210729 1882 dbSNP
rs185415164 1892 dbSNP
rs1318695393 1896 dbSNP
rs71627312 1906 dbSNP
rs540647337 1911 dbSNP
rs922186618 1915 dbSNP
rs16901282 1922 dbSNP
rs1338954630 1931 dbSNP
rs1298533491 1940 dbSNP
rs1394578333 1953 dbSNP
rs1367646922 1967 dbSNP
rs995989566 1974 dbSNP
rs1051976163 1988 dbSNP
rs1362601809 1989 dbSNP
rs574391978 1995 dbSNP
rs189352182 2011 dbSNP
rs1212136127 2022 dbSNP
rs1445634825 2029 dbSNP
rs376577504 2034 dbSNP
rs1387544915 2037 dbSNP
rs1004785190 2069 dbSNP
rs1254826372 2070 dbSNP
rs1205233441 2078 dbSNP
rs866306131 2084 dbSNP
rs1015632228 2085 dbSNP
rs961340963 2093 dbSNP
rs1188993463 2098 dbSNP
rs1249451217 2100 dbSNP
rs1452221910 2102 dbSNP
rs1340139570 2103 dbSNP
rs763150401 2107 dbSNP
rs1296339973 2109 dbSNP
rs1190319358 2113 dbSNP
rs1027033196 2120 dbSNP
rs1425237304 2124 dbSNP
rs1415744461 2127 dbSNP
rs1359124086 2129 dbSNP
rs1298118342 2138 dbSNP
rs1367824450 2146 dbSNP
rs1425910115 2146 dbSNP
rs1161868456 2147 dbSNP
rs1471580584 2148 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions TZM-bl
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM1462574. RNA binding protein: AGO2. Condition:TZM-bl ami BaL ...

- Whisnant AW; Bogerd HP; Flores O; Ho P; et al., 2013, mBio.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ucGGUGUUA----GU-GGAAGACUaga 5'
            :| ||||    || :|||||||   
Target 5' uuUCUCAAUUGGCCAGUCUUCUGA--- 3'
 		10 - 33
Article - Whisnant AW; Bogerd HP; Flores O; Ho P; et al.
- mBio, 2013
UNLABELLED: The question of how HIV-1 interfaces with cellular microRNA (miRNA) biogenesis and effector mechanisms has been highly controversial. Here, we first used deep sequencing of small RNAs present in two different infected cell lines (TZM-bl and C8166) and two types of primary human cells (CD4(+) peripheral blood mononuclear cells [PBMCs] and macrophages) to unequivocally demonstrate that HIV-1 does not encode any viral miRNAs. Perhaps surprisingly, we also observed that infection of T cells by HIV-1 has only a modest effect on the expression of cellular miRNAs at early times after infection. Comprehensive analysis of miRNA binding to the HIV-1 genome using the photoactivatable ribonucleoside-induced cross-linking and immunoprecipitation (PAR-CLIP) technique revealed several binding sites for cellular miRNAs, a subset of which were shown to be capable of mediating miRNA-mediated repression of gene expression. However, the main finding from this analysis is that HIV-1 transcripts are largely refractory to miRNA binding, most probably due to extensive viral RNA secondary structure. Together, these data demonstrate that HIV-1 neither encodes viral miRNAs nor strongly influences cellular miRNA expression, at least early after infection, and imply that HIV-1 transcripts have evolved to avoid inhibition by preexisting cellular miRNAs by adopting extensive RNA secondary structures that occlude most potential miRNA binding sites. IMPORTANCE: MicroRNAs (miRNAs) are a ubiquitous class of small regulatory RNAs that serve as posttranscriptional regulators of gene expression. Previous work has suggested that HIV-1 might subvert the function of the cellular miRNA machinery by expressing viral miRNAs or by dramatically altering the level of cellular miRNA expression. Using very sensitive approaches, we now demonstrate that neither of these ideas is in fact correct. Moreover, HIV-1 transcripts appear to largely avoid regulation by cellular miRNAs by adopting an extensive RNA secondary structure that occludes the ability of cellular miRNAs to interact with viral mRNAs. Together, these data suggest that HIV-1, rather than seeking to control miRNA function in infected cells, has instead evolved a mechanism to become largely invisible to cellular miRNA effector mechanisms.
LinkOut: [PMID: 23592263]
CLIP-seq Support 1 for dataset GSM1462574
Method / RBP PAR-CLIP / AGO2
Cell line / Condition TZM-bl / TZM-bl ami BaL
Location of target site ENST00000325366.9 | 3UTR | AAACUUGAUUUUCUCAAUUGGCCAGUCUUCUGA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23592263 / GSE59944
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1462574
Method / RBP PAR-CLIP / AGO2
Cell line / Condition TZM-bl / TZM-bl ami BaL
Location of target site ENST00000325366.9 | 3UTR | AAACUUGAUUUUCUCAAUUGGCCAGUCUUCUGA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23592263 / GSE59944
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
UCEC -0.997 0 -1.000 0.5 4 Click to see details
HNSC 0.645 0.02 0.764 0 11 Click to see details
LIHC -0.265 0.09 -0.226 0.12 28 Click to see details
BLCA 0.412 0.1 0.364 0.14 11 Click to see details
PAAD -0.943 0.11 -1.000 0.5 3 Click to see details
CHOL 0.252 0.32 0.600 0.1 6 Click to see details
STAD 0.106 0.34 0.059 0.41 18 Click to see details
PRAD 0.055 0.36 0.003 0.49 44 Click to see details
LUSC 0.19 0.38 0.100 0.44 5 Click to see details
BRCA 0.048 0.4 0.075 0.35 30 Click to see details
BRCA 0.048 0.4 0.075 0.35 30 Click to see details
BRCA 0.048 0.4 0.075 0.35 30 Click to see details
BRCA 0.048 0.4 0.075 0.35 30 Click to see details
BRCA 0.048 0.4 0.075 0.35 30 Click to see details
BRCA 0.048 0.4 0.075 0.35 30 Click to see details
BRCA 0.048 0.4 0.075 0.35 30 Click to see details
BRCA 0.048 0.4 0.075 0.35 30 Click to see details
96 hsa-miR-383-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004443 VEGFA vascular endothelial growth factor A 5 2
MIRT006137 DIO1 iodothyronine deiodinase 1 3 1
MIRT006535 IRF1 interferon regulatory factor 1 3 1
MIRT007246 IGF1R insulin like growth factor 1 receptor 1 1
MIRT007305 PRDX3 peroxiredoxin 3 1 1
MIRT054359 CCND1 cyclin D1 4 2
MIRT057087 DDIT4 DNA damage inducible transcript 4 2 2
MIRT086536 HSPE1-MOB4 HSPE1-MOB4 readthrough 2 2
MIRT086544 MOB4 MOB family member 4, phocein 2 2
MIRT438194 PPP1R10 protein phosphatase 1 regulatory subunit 10 3 1
MIRT448282 ZMYM2 zinc finger MYM-type containing 2 2 2
MIRT451601 TRPM7 transient receptor potential cation channel subfamily M member 7 2 2
MIRT453387 RHD Rh blood group D antigen 2 2
MIRT458521 C5orf22 chromosome 5 open reading frame 22 2 2
MIRT459720 SGK494 uncharacterized serine/threonine-protein kinase SgK494 2 2
MIRT461491 KIAA1009 centrosomal protein 162 1 1
MIRT463261 ZIC5 Zic family member 5 2 2
MIRT464237 VCP valosin containing protein 2 2
MIRT465320 TRAF5 TNF receptor associated factor 5 2 2
MIRT465774 TMOD3 tropomodulin 3 2 2
MIRT467879 SLC22A23 solute carrier family 22 member 23 2 2
MIRT469636 RAD21 RAD21 cohesin complex component 2 6
MIRT472041 NPAT nuclear protein, coactivator of histone transcription 2 2
MIRT476568 GABARAPL1 GABA type A receptor associated protein like 1 2 2
MIRT478586 CTDSPL2 CTD small phosphatase like 2 2 2
MIRT478870 CREBRF CREB3 regulatory factor 2 2
MIRT482202 AHR aryl hydrocarbon receptor 2 2
MIRT484237 IER2 immediate early response 2 2 2
MIRT487480 NRF1 nuclear respiratory factor 1 2 6
MIRT493746 GRAP2 GRB2-related adaptor protein 2 2 2
MIRT496836 ZNF460 zinc finger protein 460 2 2
MIRT497234 APOL2 apolipoprotein L2 2 2
MIRT499424 PLCG2 phospholipase C gamma 2 2 9
MIRT500598 UBN2 ubinuclein 2 2 10
MIRT502859 CHEK2 checkpoint kinase 2 2 6
MIRT507727 CLIC4 chloride intracellular channel 4 2 4
MIRT510509 YOD1 YOD1 deubiquitinase 2 6
MIRT511901 FNBP1L formin binding protein 1 like 2 6
MIRT514393 CLUAP1 clusterin associated protein 1 2 2
MIRT520180 WBP2 WW domain binding protein 2 2 4
MIRT531214 PLA2G4D phospholipase A2 group IVD 2 2
MIRT532846 ZNF699 zinc finger protein 699 2 2
MIRT533461 TRIM71 tripartite motif containing 71 2 2
MIRT539263 ANKRD44 ankyrin repeat domain 44 2 2
MIRT539380 ADSS adenylosuccinate synthase 2 6
MIRT548129 GAS1 growth arrest specific 1 2 2
MIRT550662 ZFP37 ZFP37 zinc finger protein 2 2
MIRT551499 CENPN centromere protein N 2 2
MIRT553778 TAF13 TATA-box binding protein associated factor 13 2 2
MIRT554376 SFPQ splicing factor proline and glutamine rich 2 2
MIRT555821 PCDH11Y protocadherin 11 Y-linked 2 4
MIRT557558 GOSR1 golgi SNAP receptor complex member 1 2 2
MIRT557694 GATA6 GATA binding protein 6 2 2
MIRT561383 TWF1 twinfilin actin binding protein 1 2 2
MIRT561414 TSN translin 2 2
MIRT561867 MSH6 mutS homolog 6 2 2
MIRT567383 GTPBP3 GTP binding protein 3, mitochondrial 2 2
MIRT569272 PCDH11X protocadherin 11 X-linked 2 2
MIRT569556 UNC119B unc-119 lipid binding chaperone B 2 2
MIRT569760 RIMBP3C RIMS binding protein 3C 2 5
MIRT573387 GGA2 golgi associated, gamma adaptin ear containing, ARF binding protein 2 2 2
MIRT573456 RPL41 ribosomal protein L41 2 2
MIRT573968 DDX21 DExD-box helicase 21 2 2
MIRT574163 ATG10 autophagy related 10 2 2
MIRT574659 KLHL15 kelch like family member 15 2 2
MIRT574907 Plcg2 phospholipase C, gamma 2 2 6
MIRT607496 HEBP2 heme binding protein 2 2 2
MIRT612840 KCNJ12 potassium voltage-gated channel subfamily J member 12 2 4
MIRT613962 TMEM59 transmembrane protein 59 2 2
MIRT621125 PCNXL2 pecanex homolog 2 2 2
MIRT625216 SH3TC2 SH3 domain and tetratricopeptide repeats 2 2 2
MIRT643337 MICA MHC class I polypeptide-related sequence A 2 2
MIRT646342 CLIC6 chloride intracellular channel 6 2 2
MIRT647361 WDR5B WD repeat domain 5B 2 2
MIRT650308 SLC35E2 solute carrier family 35 member E2 2 2
MIRT651372 ZBTB21 zinc finger and BTB domain containing 21 2 2
MIRT655301 PEAR1 platelet endothelial aggregation receptor 1 2 2
MIRT681918 SLC11A2 solute carrier family 11 member 2 2 2
MIRT684165 ALDH1B1 aldehyde dehydrogenase 1 family member B1 2 2
MIRT688053 GLUL glutamate-ammonia ligase 2 2
MIRT690210 C5orf45 MRN complex interacting protein 2 2
MIRT691024 CRTC3 CREB regulated transcription coactivator 3 2 2
MIRT697699 WAC WW domain containing adaptor with coiled-coil 2 2
MIRT698201 TMEM30A transmembrane protein 30A 2 2
MIRT698857 SRSF2 serine and arginine rich splicing factor 2 2 2
MIRT702292 LDHA lactate dehydrogenase A 4 2
MIRT703341 GDPD5 glycerophosphodiester phosphodiesterase domain containing 5 2 2
MIRT704082 DYRK2 dual specificity tyrosine phosphorylation regulated kinase 2 2 2
MIRT713729 SUCO SUN domain containing ossification factor 2 2
MIRT719487 RBM27 RNA binding motif protein 27 2 2
MIRT722080 SMC2 structural maintenance of chromosomes 2 2 2
MIRT724102 TMEM199 transmembrane protein 199 2 2
MIRT725124 SYNRG synergin gamma 2 2
MIRT725601 CAPN6 calpain 6 2 2
MIRT737186 RBM3 RNA binding motif (RNP1, RRM) protein 3 4 0
MIRT755379 NOS3 nitric oxide synthase 3 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-383 Glucose NULL 5793 Quantitative real-time PCR endothelial cells 24394957 2014 down-regulated
miR-383 Vorinostat (SAHA) approved 5311 Microarray A549 human non-small cell lung cancer cells 19513533 2009 up-regulated
miR-383 5-Fluorouracil approved 3385 Microarray CNE cells 22614822 2012 up-regulated
miR-383 Doxorubicin approved 31703 Microarray heart 22859947 2012 up-regulated
miR-383 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-383 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-383-5p Vincristine 5978 approved resistant High Lymphoblastic Leukemia tissue
hsa-miR-383-5p Daunorubicin 30323 NSC82151 approved resistant High Lymphoblastic Leukemia tissue
hsa-miR-383-5p Prednisolone 5755 NSC9120 approved resistant High Lymphoblastic Leukemia tissue
hsa-miR-383-5p L-Asparaginase resistant High Lymphoblastic Leukemia tissue
hsa-miR-383-5p Cisplatin 5460033 NSC119875 approved resistant High Germ Cell Tumor cell line (NTERA-2)
hsa-miR-383-5p Oxaliplatin 6857599 NSC266046 approved sensitive High Colorectal Cancer cell line (RKO)
hsa-miR-383-5p Oxaliplatin 6857599 NSC266046 approved resistant High Colorectal Cancer cell line (HT-29)
hsa-miR-383-5p Cisplatin 5460033 NSC119875 approved sensitive High Ovarian Cancer cell line (SKOV3)
hsa-miR-383-5p Docetaxel 148124 NSC628503 approved sensitive High Prostate Cancer cell line (PC-3)
hsa-miR-383-5p Cetuximab sensitive High Colon Cancer cell line
hsa-miR-383-5p Paclitaxel 36314 NSC125973 approved sensitive Low Ovarian Cancer cell line (OVCAR3, A2780)
hsa-miR-383-5p Doxorubicin 31703 NSC123127 approved sensitive Low Hepatocellular Carcinoma cell line (BT-474, SKBR3)
hsa-miR-383-5p Cetuximab + Folfox(Fluorouracil + Leucovorin + Oxaliplatin) resistant High Metastatic Colorectal Cancer tissue
hsa-miR-383-5p Gemcitabine 60750 NSC613327 approved sensitive Low Pancreatic Ductal Adenocarcinoma tissue
hsa-miR-383-5p Fluorouracil 3385 NSC19893 approved sensitive Low Gastric Cancer cell line (AGS)
hsa-miR-383-5p Oxaliplatin 6857599 NSC266046 approved resistant Low Hepatocellular Carcinoma cell line (Hep3B, Huh-7)
hsa-miR-383-5p Cisplatin 5460033 NSC119875 approved sensitive Low Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-383-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-383-5p Gefitinib 123631 NSC715055 approved sensitive cell line (PC9)
hsa-miR-383-5p Osimertinib 71496458 NSC779217 approved sensitive cell line (PC9)
hsa-miR-383-5p Cisplatin 5460033 NSC119875 approved sensitive cell line
hsa-miR-383-5p Cisplatin 5460033 NSC119875 approved resistant cell line (A549)
hsa-miR-383-5p Fluorouracil 3385 NSC19893 approved resistant cell line (KM12C) (72 h)
hsa-miR-383-5p Sunitinib 5329102 NSC750690 approved resistant tissue (CardA)
hsa-miR-383-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-383-5p Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)
hsa-miR-383-5p Cisplatin 5460033 NSC119875 approved resistant cell line (A2780)
hsa-miR-383-5p Cetuximab resistant tissue (colorectal carcinoma)

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