miRTarBase - #MIRT460716


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pre-miRNA Information
pre-miRNA hsa-mir-4734   
Genomic Coordinates chr17: 38702262 - 38702331
Description Homo sapiens miR-4734 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4734
Sequence 41| GCUGCGGGCUGCGGUCAGGGCG |62
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1226851679 2 dbSNP
rs1021398156 12 dbSNP
rs1011056563 13 dbSNP
rs1298210202 21 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol FN3KRP   
Synonyms FN3KL
Description fructosamine 3 kinase related protein
Transcript NM_024619   
Expression
Putative miRNA Targets on FN3KRP
3'UTR of FN3KRP
(miRNA target sites are highlighted)		 
>FN3KRP|NM_024619|3'UTR
   1 GCGGGCCTTACTCTGGAAGGAGGCCTCAGAGGTTTCTCCACAGTCCTCTTCTGGGCAAATTCTTGTTTCTTCACATGCCG
  81 GACTAGCTTAAGACCAATGCAGTAGCTTATTTCCAAGCCTTGCAAAGTATATAATATCTAAGAGGAAAGGTTTTGTCATC
 161 CCAGCGTTGTCCACTTTGTGGGGCTTTGTAGGTAGACGGAGCCACACTACAGGCAGGGTATGAGCAGAGGGATGTATGGA
 241 GTGTGGGTGACTCTGAGCCTCACTGCTGCTGCAAGGTGGGGAAACTGTAAGTGAACCCCTGTGGGTGCGGGGGAGGGTAT
 321 CCGGTGCGCAGGGAGGTGGCCAGCGCCCCCGGGCACTGCTGCTCATAGGTACCTTTCCACTGCCTCCTCCCTGCTCTCCT
 401 GTGCAGGAATGTCTCTGAGCTGTTCACGTTGATGCTTCTTGGTTGGCAAGACTTGGGTGTAGACATGAAACCATCTTACT
 481 AAAAGCGTCTTAAAATGACCAATTCCAGAATCAAGCGTATTCCGTTTTCTTCCTGCATGATCCCTGGGCCCTCCCGCAGG
 561 CTGAGCAAGTCTGTAAACTGATTCTGGGAGAAACCAAGCTGCTGGCCATAGGGTGTCCTTGGGTACATCCAGGAGTCTTC
 641 ATTGCTTCTGTTATTACCCCGTCTCCTCTGCCATTTTCTACAGCTTGCTGAGTTGTCATTCCTTTGCAACATTAAAATAC
 721 ATGCTGAACTCATATTTTTCCTTCCTTCACTGTTGTAGTAAAGAGACATATTTCATGAATGGCATTGATGCTAATAAATC
 801 CTTTGCACAAAAATTTGAATAAACTTCCAGTGGTTTCGATAATGTAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gcGGGACUGGCGUCGGGCGUCg 5'
            |||||: | |  ||||||| 
Target 5' atCCCTGGGC-CCTCCCGCAGg 3'
 540 - 560 159.00 -27.30
2 
miRNA  3' gcgggACUGG-CGUCGGGCGUCg 5'
               ||:|| || |||| |:| 
Target 5' ggaggTGGCCAGC-GCCCCCGGg 3'
 332 - 353 112.00 -18.90
3 
miRNA  3' gcGGGACUGGCGUCGGGCGUCg 5'
            |:|||:  | || || ||| 
Target 5' taCTCTGGAAGGAGGCCTCAGa 3'
 9 - 30 104.00 -21.10
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30451919 8 COSMIC
COSN30483849 21 COSMIC
COSN30477433 28 COSMIC
COSN30156707 49 COSMIC
COSN30142639 63 COSMIC
COSN19411583 66 COSMIC
COSN30143783 105 COSMIC
COSN31593174 162 COSMIC
COSN9324487 162 COSMIC
COSN22329319 192 COSMIC
COSN20114540 545 COSMIC
COSN32058069 609 COSMIC
rs1046875 380 GWAS
rs1046896 487 GWAS
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1399256846 1 dbSNP
rs774622828 3 dbSNP
rs201702321 4 dbSNP
rs1458616913 7 dbSNP
rs150881814 8 dbSNP
rs773658584 10 dbSNP
rs1484312104 14 dbSNP
rs762806453 18 dbSNP
rs996557769 19 dbSNP
rs758937034 22 dbSNP
rs1172566939 27 dbSNP
rs775451448 28 dbSNP
rs766961869 32 dbSNP
rs890808948 33 dbSNP
rs1173934460 41 dbSNP
rs1360803257 43 dbSNP
rs1448172662 45 dbSNP
rs754291026 47 dbSNP
rs762622947 49 dbSNP
rs981340698 51 dbSNP
rs1233964875 59 dbSNP
rs1205523494 66 dbSNP
rs1299306473 70 dbSNP
rs1218676245 76 dbSNP
rs1362931018 79 dbSNP
rs2243446 80 dbSNP
rs560293370 81 dbSNP
rs990811972 82 dbSNP
rs1365011706 85 dbSNP
rs1201300992 91 dbSNP
rs956231553 99 dbSNP
rs916583249 101 dbSNP
rs1174782183 102 dbSNP
rs1409982721 103 dbSNP
rs1261095294 105 dbSNP
rs1421008733 111 dbSNP
rs990760842 119 dbSNP
rs1448466998 123 dbSNP
rs1021812470 125 dbSNP
rs941465616 127 dbSNP
rs1459946748 135 dbSNP
rs370972441 136 dbSNP
rs76587147 141 dbSNP
rs1249771309 145 dbSNP
rs980200937 161 dbSNP
rs1308216904 166 dbSNP
rs146163729 167 dbSNP
rs1364705045 172 dbSNP
rs1316199384 184 dbSNP
rs1172722689 185 dbSNP
rs1377638574 191 dbSNP
rs1393105745 194 dbSNP
rs992069694 195 dbSNP
rs528835266 198 dbSNP
rs1428727391 199 dbSNP
rs1385424128 201 dbSNP
rs1165241141 208 dbSNP
rs1442492080 211 dbSNP
rs1051350167 218 dbSNP
rs891350096 221 dbSNP
rs1038131349 226 dbSNP
rs942925671 228 dbSNP
rs570401293 231 dbSNP
rs1172840823 233 dbSNP
rs1234726012 234 dbSNP
rs919760796 240 dbSNP
rs1039818733 243 dbSNP
rs1292690198 254 dbSNP
rs58962659 261 dbSNP
rs72046641 261 dbSNP
rs1322002954 262 dbSNP
rs1320364318 269 dbSNP
rs892787544 270 dbSNP
rs1312414599 272 dbSNP
rs890780831 277 dbSNP
rs1376101896 278 dbSNP
rs537853921 279 dbSNP
rs1008350154 285 dbSNP
rs771337712 286 dbSNP
rs1304646666 297 dbSNP
rs1011772238 298 dbSNP
rs1214834540 299 dbSNP
rs1020007866 304 dbSNP
rs112990290 306 dbSNP
rs549670852 306 dbSNP
rs1440834124 307 dbSNP
rs1002625815 308 dbSNP
rs556745340 309 dbSNP
rs1035477893 310 dbSNP
rs958654421 316 dbSNP
rs535519039 323 dbSNP
rs1234212537 324 dbSNP
rs563003827 328 dbSNP
rs11551832 329 dbSNP
rs1487614120 331 dbSNP
rs974582210 338 dbSNP
rs1042869128 339 dbSNP
rs960065641 345 dbSNP
rs1268865577 346 dbSNP
rs1465282635 349 dbSNP
rs572258264 351 dbSNP
rs930137048 352 dbSNP
rs1384770196 355 dbSNP
rs775986588 356 dbSNP
rs987001210 362 dbSNP
rs912735484 365 dbSNP
rs1386377460 366 dbSNP
rs907878098 372 dbSNP
rs748900999 378 dbSNP
rs942958189 379 dbSNP
rs1046875 380 dbSNP
rs571818746 386 dbSNP
rs1266853357 389 dbSNP
rs576216402 396 dbSNP
rs1206100961 397 dbSNP
rs947032271 399 dbSNP
rs932513860 402 dbSNP
rs1266626457 404 dbSNP
rs1208699034 408 dbSNP
rs1041864532 420 dbSNP
rs193216896 422 dbSNP
rs140176973 426 dbSNP
rs1002573464 428 dbSNP
rs570537411 429 dbSNP
rs1383555718 438 dbSNP
rs1490606359 463 dbSNP
rs1035510845 464 dbSNP
rs1046889 465 dbSNP
rs1324531766 474 dbSNP
rs1013261728 485 dbSNP
rs1046896 487 dbSNP
rs1387672003 488 dbSNP
rs1043358978 501 dbSNP
rs1174392758 512 dbSNP
rs963037510 513 dbSNP
rs995486165 517 dbSNP
rs1026057929 518 dbSNP
rs951484881 524 dbSNP
rs143639702 525 dbSNP
rs3087795 530 dbSNP
rs184357380 534 dbSNP
rs912766522 537 dbSNP
rs552639315 541 dbSNP
rs66482220 542 dbSNP
rs1229216896 549 dbSNP
rs57335410 556 dbSNP
rs549876039 557 dbSNP
rs568178549 558 dbSNP
rs1332176143 564 dbSNP
rs1395382715 567 dbSNP
rs947616934 568 dbSNP
rs7208281 570 dbSNP
rs1168548991 576 dbSNP
rs1041420268 577 dbSNP
rs924261249 578 dbSNP
rs1445554935 583 dbSNP
rs1249572944 584 dbSNP
rs1471397488 585 dbSNP
rs746839593 587 dbSNP
rs1183981765 588 dbSNP
rs1412038306 596 dbSNP
rs1474567773 602 dbSNP
rs1162127765 608 dbSNP
rs1046917 609 dbSNP
rs894330026 610 dbSNP
rs7794 614 dbSNP
rs1038032074 616 dbSNP
rs1297955505 617 dbSNP
rs565587577 618 dbSNP
rs1394873563 619 dbSNP
rs1411868588 631 dbSNP
rs947060897 635 dbSNP
rs1336230843 637 dbSNP
rs747430509 642 dbSNP
rs762031695 651 dbSNP
rs1043325759 658 dbSNP
rs906147621 661 dbSNP
rs1231483164 662 dbSNP
rs899116971 666 dbSNP
rs993820088 673 dbSNP
rs539712281 687 dbSNP
rs1364812660 699 dbSNP
rs1183729739 700 dbSNP
rs1471355907 705 dbSNP
rs1245502427 713 dbSNP
rs12296 720 dbSNP
rs1005345111 737 dbSNP
rs77688232 738 dbSNP
rs769835685 744 dbSNP
rs1351317440 751 dbSNP
rs146773542 752 dbSNP
rs1226484134 758 dbSNP
rs1348780076 759 dbSNP
rs1287783488 762 dbSNP
rs1351792203 766 dbSNP
rs964213789 766 dbSNP
rs1252039663 768 dbSNP
rs975640003 768 dbSNP
rs994833006 769 dbSNP
rs914720787 772 dbSNP
rs969019433 775 dbSNP
rs978064207 777 dbSNP
rs1405073964 784 dbSNP
rs1386110768 786 dbSNP
rs1162078758 789 dbSNP
rs1471877074 792 dbSNP
rs1410652736 793 dbSNP
rs1485143561 797 dbSNP
rs924292386 806 dbSNP
rs938325491 814 dbSNP
rs1478826343 816 dbSNP
rs953927776 818 dbSNP
rs1244557525 830 dbSNP
rs1197922490 833 dbSNP
rs1056810997 834 dbSNP
rs1447422725 839 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions TZM-bl
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM1462574. RNA binding protein: AGO2. Condition:TZM-bl ami BaL ...

- Whisnant AW; Bogerd HP; Flores O; Ho P; et al., 2013, mBio.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' gcGGGACUGGCGUCGGGCGUcg 5'
            ::| | :: |  ||:|||  
Target 5' uaUUCCGUUUUCUUCCUGCAug 3'
 		1 - 22
Article - Whisnant AW; Bogerd HP; Flores O; Ho P; et al.
- mBio, 2013
UNLABELLED: The question of how HIV-1 interfaces with cellular microRNA (miRNA) biogenesis and effector mechanisms has been highly controversial. Here, we first used deep sequencing of small RNAs present in two different infected cell lines (TZM-bl and C8166) and two types of primary human cells (CD4(+) peripheral blood mononuclear cells [PBMCs] and macrophages) to unequivocally demonstrate that HIV-1 does not encode any viral miRNAs. Perhaps surprisingly, we also observed that infection of T cells by HIV-1 has only a modest effect on the expression of cellular miRNAs at early times after infection. Comprehensive analysis of miRNA binding to the HIV-1 genome using the photoactivatable ribonucleoside-induced cross-linking and immunoprecipitation (PAR-CLIP) technique revealed several binding sites for cellular miRNAs, a subset of which were shown to be capable of mediating miRNA-mediated repression of gene expression. However, the main finding from this analysis is that HIV-1 transcripts are largely refractory to miRNA binding, most probably due to extensive viral RNA secondary structure. Together, these data demonstrate that HIV-1 neither encodes viral miRNAs nor strongly influences cellular miRNA expression, at least early after infection, and imply that HIV-1 transcripts have evolved to avoid inhibition by preexisting cellular miRNAs by adopting extensive RNA secondary structures that occlude most potential miRNA binding sites. IMPORTANCE: MicroRNAs (miRNAs) are a ubiquitous class of small regulatory RNAs that serve as posttranscriptional regulators of gene expression. Previous work has suggested that HIV-1 might subvert the function of the cellular miRNA machinery by expressing viral miRNAs or by dramatically altering the level of cellular miRNA expression. Using very sensitive approaches, we now demonstrate that neither of these ideas is in fact correct. Moreover, HIV-1 transcripts appear to largely avoid regulation by cellular miRNAs by adopting an extensive RNA secondary structure that occludes the ability of cellular miRNAs to interact with viral mRNAs. Together, these data suggest that HIV-1, rather than seeking to control miRNA function in infected cells, has instead evolved a mechanism to become largely invisible to cellular miRNA effector mechanisms.
LinkOut: [PMID: 23592263]
CLIP-seq Support 1 for dataset GSM1462574
Method / RBP PAR-CLIP / AGO2
Cell line / Condition TZM-bl / TZM-bl ami BaL
Location of target site ENST00000269373.6 | 3UTR | UAUUCCGUUUUCUUCCUGCAUGAUCCCUGGGCCCUCCCG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23592263 / GSE59944
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
15 hsa-miR-4734 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT460716 FN3KRP fructosamine 3 kinase related protein 2 2
MIRT467603 SLC7A5 solute carrier family 7 member 5 2 2
MIRT477489 ELL2 elongation factor for RNA polymerase II 2 2 2
MIRT481373 ATG9A autophagy related 9A 2 2
MIRT486420 RXRA retinoid X receptor alpha 2 2
MIRT491673 AQP2 aquaporin 2 2 2
MIRT504626 SCIN scinderin 2 6
MIRT516768 FAM212B family with sequence similarity 212 member B 2 4
MIRT570599 NFIX nuclear factor I X 2 2
MIRT608761 CACNA1A calcium voltage-gated channel subunit alpha1 A 2 2
MIRT615150 URGCP-MRPS24 URGCP-MRPS24 readthrough 2 2
MIRT617122 ZNF556 zinc finger protein 556 2 2
MIRT628615 ZBTB3 zinc finger and BTB domain containing 3 2 2
MIRT702726 INSIG1 insulin induced gene 1 2 2
MIRT712897 TGFA transforming growth factor alpha 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4734 Dabrafenib 44462760 NSC764134 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-4734 Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-4734 Doxorubicin 31703 NSC123127 approved sensitive High Breast Cancer cell line (MCF-7)
hsa-miR-4734 Curcumin 969516 NSC32982 approved sensitive High Breast Cancer cell line (MCF-7)
hsa-miR-4734 Paclitaxel 36314 NSC125973 approved resistant High Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-4734 Tamoxifen 2733525 NSC180973 approved resistant High Breast Cancer cell line (MCF-7)
hsa-miR-4734 Fulvestrant 17756771 NSC719276 approved sensitive High Breast Cancer cell line (MCF-7)
hsa-miR-4734 Gefitinib 123631 NSC715055 approved sensitive High Non-Small Cell Lung Cancer cell line (PC-9)
hsa-miR-4734 Gemcitabine 60750 NSC613327 approved sensitive High Pancreatic Cancer cell line (AsPC-1)
hsa-miR-4734 Oxaliplatin 6857599 NSC266046 approved resistant Low Colorectal Cancer cell line (SW480, HCT-116)
hsa-mir-4734 Dabrafenib 44462760 NSC764134 approved resistant cell line (A375)
hsa-mir-4734 Doxorubicin 31703 NSC123127 approved resistant cell line (BAS)
hsa-mir-4734 Cisplatin 5460033 NSC119875 approved resistant cell line (A2780)
hsa-mir-4734 Cisplatin 5460033 NSC119875 approved resistant cell line (BxPC3)
hsa-miR-4734 Cisplatin 5460033 NSC119875 approved sensitive cell line
hsa-miR-4734 Cisplatin 5460033 NSC119875 approved resistant cell line (A549)
hsa-miR-4734 Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-4734 Paclitaxel 36314 NSC125973 approved sensitive cell line (BAS)
hsa-miR-4734 Doxorubicin 31703 NSC123127 approved resistant cell line (BAS)
hsa-miR-4734 Doxorubicin 31703 NSC123127 approved resistant cell line (HS578T)
hsa-miR-4734 Cisplatin 5460033 NSC119875 approved resistant cell line (CIS)
hsa-miR-4734 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)

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