miRTarBase - #MIRT464637


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pre-miRNA Information
pre-miRNA hsa-mir-514a-1   
Genomic Coordinates chrX: 147279247 - 147279344
Description Homo sapiens miR-514a-1 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure
Associated Diseases
pre-miRNA hsa-mir-514a-2   
Genomic Coordinates chrX: 147281943 - 147282030
Description Homo sapiens miR-514a-2 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure
Associated Diseases
pre-miRNA hsa-mir-514a-3   
Genomic Coordinates chrX: 147284641 - 147284728
Description Homo sapiens miR-514a-3 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-514a-5p
Sequence 22| UACUCUGGAGAGUGACAAUCAUG |44
Evidence Not_experimental
Experiments
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs782234889 2 dbSNP
rs782394076 2 dbSNP
rs1198095985 3 dbSNP
rs1421970977 9 dbSNP
rs1477599888 11 dbSNP
rs781957284 14 dbSNP
rs782333375 15 dbSNP
rs1254451608 15 dbSNP
rs183412647 16 dbSNP
rs377644523 18 dbSNP
rs782689393 19 dbSNP
rs782118062 23 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol UBE4A   
Synonyms E4, UBOX2, UFD2
Description ubiquitination factor E4A
Transcript NM_004788   
Expression
Putative miRNA Targets on UBE4A
3'UTR of UBE4A
(miRNA target sites are highlighted)		 
>UBE4A|NM_004788|3'UTR
   1 ATACTGTGAACTAACCAAACCAAAACCAACCCCAGAGTGCAGATAAACAATTGTTTGTGGTTTCTCTCTTTCTGGTTCTG
  81 TTCCTTTTCTTTCTTCTTTTCTTTTTCTTTTTTTTTTTTTTTTTTACTAAATTAGAGAACTGCTCTTGCTGAAATTATGA
 161 TAGTTAAGATTCCTAAGAACTTGACAATGCTCCCCTGGCTTGCAGGAAATTATACTTATTATAGCATCACCAAGTTTAGA
 241 AATCATCACTAATTTTCACCCTCTGTTGTCTCTTCATATTCTTCTTCCTTTTCCTAAATGAAATTATATTATTTCAACTA
 321 CTAAAACTTCCCGCCACCCTGCTATTTCTCTTCCAATTACTGTTCAAACATTTTTGGTGAGTGCTGCTTTTATAAATATG
 401 TGATGTCACATATTTCAGTGACAGCTGATGTTATCAGAAAAATCCTGTTATCCTGTGTATTTACTAGTCCTCATGATCTA
 481 GACTTAACCCCTTTTCTGTGTTACAGAGTAACTTCACATAAAAGCAGATACCTTTAAGTTTGTGTAGACTTCTGAATAAG
 561 ATGATAGACGAGAATTTTTTTTAAAAAAGGAATTTAAAGGAATCTTGTGCAACTTCTGTTGATAGCTCTTAATTTTGTTA
 641 TACAGTCTTTTTAATGAGGATTGGTAGGGCAATCCTACCAATTGATGGGGGTGAGGAGACTGTTGGGCCCTTATTCTCTT
 721 ATGAAAATCTGTTTCTACAAGGACTAGGCCTAAGTAGATTTAGGCAACGGGTATCATATCCATGAAAAATGTCATTTTAA
 801 GACACTAATATCAACAGTATATCTCAGTGTGTGAGATATATAAATACACATACACCCCATGTGCTTTTTTGTTGGTTTAG
 881 TGTAAACTGAGTACCACTTTATATTTTCTCTTCCATAGTGAGATGTATGCAGTATGTGGCCATGTTTACTAACATACTCA
 961 TTCTTCACAAAATTCTGAGATTATAAAATGTATATAGTTAATATTTGTTTGAGCTTGTGACCTGACTTCTAAGAGCTACT
1041 TCTAACACAGCCTTAGGTCTTCAATTAACAAAGTAGGAAGTTGAGCACAACCTTGTCCACAGCCATTTTAAATATCCACT
1121 TAGCCCTGTGTAGTTGGTAGGTGGACACCACTTTCATAAGTGAACTTGAGCTCACTACTCAATTGTGCAGCTTAATATGC
1201 TGACTAGGGACATTCCCCTATGGATTATCTTTATATCACTGTCTTTCTAAGCCCAGAGATGTCATAAGTGACAAGAAAAG
1281 TGATAGGATCAAGAAATGGGTGTCACTGCTTCATTTGGAACATGTTATTAACTATGGTCTCTTTCAAATAAATAGTAGTT
1361 TTATATTTCAACACAAGTTGCTATAAGCAGTCCTTGATGGGTTTTTGATTGCATCAGCTGGAAAGCCTCAAATCTAAGAG
1441 GGCTTCCCATCCTAGATATAAAATAGGTGTTGCCTATTGCTGTGCTTATAAAATGAAAAAGGAAATTGAGGACACTTTTG
1521 CAAATGCCAGAATGTAAGATTCATTCAGTGTGCTCCCTGGGCCTTTATGGCATGGGTTGACAGGATTTGTTTATTTTCTA
1601 AAATTAGCTTCATTCAATATTTATCATCCTCCTTTCCCTCTCTGAGAATGAACTATGTATAAAATAAGCTTCTGCCTATT
1681 TGCATTTATCTTCCAAACCCAATCTAGTAGGATGTTCTCATTTTAAAAACGAGGGGAAAAGACCAGAGTTTTTCAGGAGA
1761 AAACTGGAGGAAAATGGGCACAAAAACTCAGAAGGCAGCTATTCCCAGCAGCTTCCTAGTTAACAACCCCCATGCTGCCT
1841 CCAGTCTTTGTCTGTATTCTTCTGTATTTAACCTTCAGATTGTAAGCCTTTTCTGGCAAGCTTTTCTTCTTTTTTTAAAC
1921 TCTTTTCCTGAAACTTTTTATGAATGGCTATGGCACCATTAATGCTGCTGAATATCTTTAAACTCTGCACAAGCAAGTGT
2001 GTAGCTTAAGGCCACTACTGGTAAGGAAACCAAGTGTCCTCTGTGCCTTTTTTCTTTCTGTGAAGTAATTTAAGAATATC
2081 CAAAAAAATTAGACTTTAAAAAGTTATCTTGGTACAACACCGTGTGTATATACACTTGGAAGCTTAAAAAGGTGTTTTGT
2161 CTGGAACTTAGAAGCAGCTCTAAATCTAGTAGAGCAGACTTTCTAACATACCTAGTTTTGTGTATTGGCTTTGCTGGAGT
2241 ATGATAGCAAAATGAAGACTCTTTTACTCAGCTCTGGTATTGCTCATAACTTACCAAGAGGCTAATACTAAACTTGGAAA
2321 ATTGTTTAAGTATGTTTTATCAAGCAGTCTGGGTTTTGTTTTTTAATATACTTTTTAATGGATATGTGAAAACTGAAGGA
2401 AATGTTAAAGGTTTTTTAATGGTGCAAGTGAAGGTGCCAGTTGCTATTTGATATCACACTCTACAAAAGCTTCATTACTT
2481 TATTTGATGGTGGTTGCTAAGCAGCCATTGCACAGAGCATAAGTCTACTGGGTGCCTTTACATGCCAGAGGCTGATGCTG
2561 CACTGTTGATGTCATGTGAGGAAATAATGCACATGCTCTAACTGCTCAACAGGAAATGAACCTAGAAACAGAAAATGAAA
2641 AGGTTGATTGAAATAAAACTTGATCAACGCGACTGTATTTTGAAACATTCCAGGAAGGTTACTTCTTGTCAAACTTGCCT
2721 GGCAGTGTTTGTTCAAAACTTGTATTTAATAAATGAACATCTGACTTACAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' guACUAACAGUGAGA----GGUCUCau 5'
            | | |||| :|||    ||||||  
Target 5' taTCACTGTCTTTCTAAGCCCAGAGat 3'
 1234 - 1260 143.00 -14.20
2 
miRNA  3' guacuaacagugaGAGGUCUCAu 5'
                       | ||||||| 
Target 5' aaaccaaaaccaaCCCCAGAGTg 3'
 17 - 39 142.00 -12.10
3 
miRNA  3' guacuaacagugagaGGUCUCAu 5'
                         ||||||| 
Target 5' aacgaggggaaaagaCCAGAGTt 3'
 1728 - 1750 140.00 -11.40
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN31562283 4 COSMIC
COSN30504397 24 COSMIC
COSN30100438 69 COSMIC
COSN30514453 92 COSMIC
COSN31527058 92 COSMIC
COSN19729263 102 COSMIC
COSN30498774 107 COSMIC
COSN19621771 108 COSMIC
COSN31527551 138 COSMIC
COSN32056324 223 COSMIC
COSN32055558 262 COSMIC
COSN26321579 569 COSMIC
COSN6988161 940 COSMIC
COSN19595637 1057 COSMIC
COSN22422220 1649 COSMIC
COSN20092432 1752 COSMIC
COSN31585548 1884 COSMIC
COSN1515782 1945 COSMIC
COSN26576213 1968 COSMIC
COSN32134718 1971 COSMIC
COSN16108220 2090 COSMIC
COSN31547238 2090 COSMIC
COSN31960697 2122 COSMIC
COSN31590160 2123 COSMIC
COSN6531339 2341 COSMIC
COSN28855509 2578 COSMIC
COSN26556995 2698 COSMIC
rs45448191 583 GWAS
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1452310345 3 dbSNP
rs373842700 3 dbSNP
rs377002812 4 dbSNP
rs1403456106 11 dbSNP
rs782660542 12 dbSNP
rs782183742 27 dbSNP
rs782330149 30 dbSNP
rs548810839 31 dbSNP
rs782378899 32 dbSNP
rs532108389 33 dbSNP
rs782005140 33 dbSNP
rs115395500 35 dbSNP
rs782812330 39 dbSNP
rs781918587 47 dbSNP
rs782063399 49 dbSNP
rs1182784926 51 dbSNP
rs782718068 51 dbSNP
rs781832318 53 dbSNP
rs1459771406 69 dbSNP
rs537606677 70 dbSNP
rs557443379 76 dbSNP
rs571293243 82 dbSNP
rs1197847801 84 dbSNP
rs1340306350 84 dbSNP
rs1784250 90 dbSNP
rs1019251544 93 dbSNP
rs1225296145 95 dbSNP
rs880003651 98 dbSNP
rs1316274851 100 dbSNP
rs1381122929 102 dbSNP
rs1792838 102 dbSNP
rs1333767604 104 dbSNP
rs377551828 107 dbSNP
rs1182235131 108 dbSNP
rs1382003722 108 dbSNP
rs1385805854 108 dbSNP
rs1391660487 108 dbSNP
rs1438650812 108 dbSNP
rs1442698149 108 dbSNP
rs1491006942 108 dbSNP
rs472243 108 dbSNP
rs571514647 108 dbSNP
rs5795126 108 dbSNP
rs1250019797 111 dbSNP
rs1194162735 112 dbSNP
rs1490840821 112 dbSNP
rs1245964272 113 dbSNP
rs1292579312 113 dbSNP
rs1357896186 114 dbSNP
rs1311969131 120 dbSNP
rs1240155171 126 dbSNP
rs1334828301 127 dbSNP
rs1388014768 131 dbSNP
rs1365730756 140 dbSNP
rs1301979380 150 dbSNP
rs1422725270 159 dbSNP
rs533581331 160 dbSNP
rs1158965973 161 dbSNP
rs1469382918 171 dbSNP
rs759026488 188 dbSNP
rs1180007119 190 dbSNP
rs1481533934 193 dbSNP
rs189755354 197 dbSNP
rs1490011373 202 dbSNP
rs1286899997 203 dbSNP
rs1206106853 210 dbSNP
rs573497806 215 dbSNP
rs1350327826 220 dbSNP
rs1278144002 221 dbSNP
rs1225033317 227 dbSNP
rs1345730537 251 dbSNP
rs1282136945 252 dbSNP
rs1402512412 260 dbSNP
rs975135333 262 dbSNP
rs1342304679 264 dbSNP
rs1318808102 279 dbSNP
rs1400032409 318 dbSNP
rs782697091 319 dbSNP
rs1174086315 324 dbSNP
rs1471101119 331 dbSNP
rs542538536 333 dbSNP
rs374067720 334 dbSNP
rs977350147 345 dbSNP
rs1244340612 352 dbSNP
rs1194604688 366 dbSNP
rs1446915619 368 dbSNP
rs1282662453 388 dbSNP
rs556051082 393 dbSNP
rs1323855567 409 dbSNP
rs550670331 411 dbSNP
rs1221156691 419 dbSNP
rs1340942938 428 dbSNP
rs1270252874 429 dbSNP
rs1429994117 450 dbSNP
rs1324585896 452 dbSNP
rs1056452315 457 dbSNP
rs896498031 470 dbSNP
rs1453034440 471 dbSNP
rs1407132802 482 dbSNP
rs1175888655 490 dbSNP
rs1468427253 497 dbSNP
rs1421812933 503 dbSNP
rs948082108 507 dbSNP
rs1475399482 513 dbSNP
rs1240826157 526 dbSNP
rs1185839261 531 dbSNP
rs79990075 532 dbSNP
rs1242911711 540 dbSNP
rs1201446073 545 dbSNP
rs1308867696 552 dbSNP
rs1273148032 560 dbSNP
rs1213435252 563 dbSNP
rs147197772 570 dbSNP
rs533445760 571 dbSNP
rs1246446719 574 dbSNP
rs1334432791 583 dbSNP
rs45448191 583 dbSNP
rs1446153962 584 dbSNP
rs1356469868 591 dbSNP
rs139602989 592 dbSNP
rs1409405588 596 dbSNP
rs10892237 609 dbSNP
rs1166088480 613 dbSNP
rs1445748330 614 dbSNP
rs1385054419 625 dbSNP
rs1182098805 629 dbSNP
rs535573931 644 dbSNP
rs1481428129 645 dbSNP
rs1233046692 647 dbSNP
rs963720132 648 dbSNP
rs1293317120 649 dbSNP
rs1223211686 651 dbSNP
rs975123654 660 dbSNP
rs1312704917 664 dbSNP
rs1225223455 668 dbSNP
rs1375830010 671 dbSNP
rs1280303728 673 dbSNP
rs1441741790 678 dbSNP
rs533846918 697 dbSNP
rs1021356555 699 dbSNP
rs753625479 711 dbSNP
rs1305005416 716 dbSNP
rs968560699 718 dbSNP
rs193148462 723 dbSNP
rs1365462000 737 dbSNP
rs924480975 741 dbSNP
rs782082879 744 dbSNP
rs1408269221 745 dbSNP
rs45480805 749 dbSNP
rs1468272420 762 dbSNP
rs1199470648 769 dbSNP
rs992748731 769 dbSNP
rs1490176111 772 dbSNP
rs1289449930 785 dbSNP
rs1217695270 791 dbSNP
rs1317224751 795 dbSNP
rs1283274907 798 dbSNP
rs531424516 808 dbSNP
rs948070861 819 dbSNP
rs1045160982 820 dbSNP
rs1285305254 826 dbSNP
rs1405579100 827 dbSNP
rs920028553 832 dbSNP
rs1345448148 833 dbSNP
rs1292223401 834 dbSNP
rs551139359 836 dbSNP
rs931433480 840 dbSNP
rs1346222853 841 dbSNP
rs1158808327 842 dbSNP
rs1047638937 850 dbSNP
rs1455354109 854 dbSNP
rs1430927764 857 dbSNP
rs1199546197 860 dbSNP
rs1479792505 864 dbSNP
rs1266693243 865 dbSNP
rs1188265972 867 dbSNP
rs1486140012 875 dbSNP
rs1241343072 899 dbSNP
rs1213333210 902 dbSNP
rs571287795 904 dbSNP
rs1265167010 911 dbSNP
rs1224206201 915 dbSNP
rs184664028 921 dbSNP
rs1323005637 925 dbSNP
rs1299268248 928 dbSNP
rs55987000 941 dbSNP
rs765194156 952 dbSNP
rs547125242 966 dbSNP
rs1363235589 984 dbSNP
rs1288688239 987 dbSNP
rs1433290977 991 dbSNP
rs35394586 1001 dbSNP
rs1407866770 1004 dbSNP
rs45527137 1005 dbSNP
rs1468966486 1015 dbSNP
rs1375473987 1030 dbSNP
rs1171861795 1032 dbSNP
rs1429076275 1065 dbSNP
rs567258021 1070 dbSNP
rs1186460010 1073 dbSNP
rs1462433582 1075 dbSNP
rs1243522257 1077 dbSNP
rs1204614585 1080 dbSNP
rs1461023366 1085 dbSNP
rs1254380585 1086 dbSNP
rs782218722 1091 dbSNP
rs782361964 1116 dbSNP
rs1308626123 1124 dbSNP
rs1295424911 1129 dbSNP
rs1226657991 1134 dbSNP
rs1385524094 1155 dbSNP
rs1337773478 1156 dbSNP
rs1451509847 1157 dbSNP
rs1399555412 1158 dbSNP
rs1299402903 1163 dbSNP
rs1461998550 1179 dbSNP
rs1372434659 1186 dbSNP
rs189132710 1192 dbSNP
rs1166712303 1196 dbSNP
rs1446356324 1202 dbSNP
rs536213890 1203 dbSNP
rs1182721017 1208 dbSNP
rs556110770 1211 dbSNP
rs1254282251 1218 dbSNP
rs1197647150 1221 dbSNP
rs1480964765 1226 dbSNP
rs781994768 1228 dbSNP
rs1203712055 1231 dbSNP
rs1362176602 1238 dbSNP
rs1316206460 1248 dbSNP
rs1245633807 1275 dbSNP
rs1338347581 1276 dbSNP
rs1308611882 1279 dbSNP
rs1391607837 1281 dbSNP
rs1395215019 1287 dbSNP
rs1305619870 1300 dbSNP
rs1426519766 1306 dbSNP
rs1366070151 1308 dbSNP
rs1162721842 1313 dbSNP
rs1456147954 1329 dbSNP
rs1366944008 1330 dbSNP
rs1184337095 1335 dbSNP
rs1419886584 1341 dbSNP
rs1251220544 1345 dbSNP
rs1180049816 1350 dbSNP
rs1490989474 1351 dbSNP
rs1293063510 1352 dbSNP
rs1209620562 1353 dbSNP
rs1354766227 1354 dbSNP
rs575982936 1362 dbSNP
rs782267678 1374 dbSNP
rs1262849971 1379 dbSNP
rs1239380613 1380 dbSNP
rs1334773544 1382 dbSNP
rs1285948733 1383 dbSNP
rs1406195889 1392 dbSNP
rs1346088389 1393 dbSNP
rs1302401427 1398 dbSNP
rs1403533035 1400 dbSNP
rs782420176 1408 dbSNP
rs1158911125 1411 dbSNP
rs1454528017 1418 dbSNP
rs1411442947 1420 dbSNP
rs1157047536 1426 dbSNP
rs477027 1456 dbSNP
rs7108613 1459 dbSNP
rs1269689209 1463 dbSNP
rs1198847103 1477 dbSNP
rs1450371064 1480 dbSNP
rs1206047827 1481 dbSNP
rs1328075241 1482 dbSNP
rs1265790098 1491 dbSNP
rs1224827016 1516 dbSNP
rs1323710592 1524 dbSNP
rs1273724432 1530 dbSNP
rs782066286 1534 dbSNP
rs1328083922 1539 dbSNP
rs1318748229 1565 dbSNP
rs1381382741 1567 dbSNP
rs1388182560 1575 dbSNP
rs1318862531 1580 dbSNP
rs1430134160 1586 dbSNP
rs1471069967 1586 dbSNP
rs181831283 1594 dbSNP
rs1477344808 1598 dbSNP
rs781968384 1606 dbSNP
rs782115168 1609 dbSNP
rs1464677972 1613 dbSNP
rs1246573454 1614 dbSNP
rs1205244558 1618 dbSNP
rs1461476771 1623 dbSNP
rs1277355945 1628 dbSNP
rs186445215 1630 dbSNP
rs540722573 1631 dbSNP
rs1341399473 1638 dbSNP
rs1270627522 1639 dbSNP
rs1362836025 1653 dbSNP
rs149747457 1653 dbSNP
rs782772492 1654 dbSNP
rs1338428657 1656 dbSNP
rs1454817883 1677 dbSNP
rs1407060330 1687 dbSNP
rs1333619093 1689 dbSNP
rs1412778201 1696 dbSNP
rs573710362 1700 dbSNP
rs1399266635 1703 dbSNP
rs1169069098 1709 dbSNP
rs1449382351 1712 dbSNP
rs1388763489 1721 dbSNP
rs1185782166 1728 dbSNP
rs1444884391 1730 dbSNP
rs537830357 1731 dbSNP
rs1207416259 1732 dbSNP
rs189287604 1742 dbSNP
rs1204405648 1745 dbSNP
rs554845199 1752 dbSNP
rs1316935928 1755 dbSNP
rs1246377511 1766 dbSNP
rs61900934 1770 dbSNP
rs1380184527 1771 dbSNP
rs1283752439 1776 dbSNP
rs531486662 1780 dbSNP
rs1337674555 1782 dbSNP
rs551325240 1789 dbSNP
rs1429720454 1800 dbSNP
rs1369209199 1802 dbSNP
rs1165874668 1807 dbSNP
rs1425613845 1807 dbSNP
rs1413518340 1808 dbSNP
rs1179812969 1815 dbSNP
rs1470945770 1816 dbSNP
rs1232992608 1817 dbSNP
rs1180775299 1818 dbSNP
rs1482045435 1819 dbSNP
rs1293781095 1822 dbSNP
rs1222962351 1828 dbSNP
rs1320966662 1844 dbSNP
rs1287455885 1846 dbSNP
rs1226873461 1864 dbSNP
rs1353080181 1866 dbSNP
rs1289105451 1867 dbSNP
rs1409219283 1872 dbSNP
rs1349177857 1880 dbSNP
rs1305557213 1883 dbSNP
rs1437787613 1898 dbSNP
rs1349710287 1906 dbSNP
rs1163081363 1907 dbSNP
rs1402115321 1916 dbSNP
rs1408201055 1917 dbSNP
rs565086530 1925 dbSNP
rs1471286803 1935 dbSNP
rs527449105 1951 dbSNP
rs1200133809 1973 dbSNP
rs1488106011 1975 dbSNP
rs1217634890 1984 dbSNP
rs1318982683 1985 dbSNP
rs1268924558 1995 dbSNP
rs781814109 2031 dbSNP
rs547479420 2043 dbSNP
rs1327231078 2045 dbSNP
rs1285244842 2047 dbSNP
rs1220352113 2048 dbSNP
rs1292167989 2048 dbSNP
rs1433255993 2053 dbSNP
rs781956196 2072 dbSNP
rs1319554963 2076 dbSNP
rs567215635 2079 dbSNP
rs556654124 2082 dbSNP
rs1430857329 2090 dbSNP
rs181030112 2091 dbSNP
rs1432558327 2095 dbSNP
rs1422921482 2098 dbSNP
rs1188048527 2111 dbSNP
rs1486108314 2115 dbSNP
rs1247167121 2123 dbSNP
rs1208398302 2127 dbSNP
rs1463686732 2132 dbSNP
rs186514506 2140 dbSNP
rs1210846340 2142 dbSNP
rs1312136857 2144 dbSNP
rs1299736729 2151 dbSNP
rs1216825921 2161 dbSNP
rs1366449556 2171 dbSNP
rs782628761 2184 dbSNP
rs1432504073 2189 dbSNP
rs1407800659 2195 dbSNP
rs1302948297 2197 dbSNP
rs1465061507 2211 dbSNP
rs1356922446 2213 dbSNP
rs782251027 2216 dbSNP
rs879966945 2218 dbSNP
rs782540232 2225 dbSNP
rs1449978746 2230 dbSNP
rs1389365551 2232 dbSNP
rs1186394444 2236 dbSNP
rs1447912563 2242 dbSNP
rs1201112995 2246 dbSNP
rs1483005811 2252 dbSNP
rs1254319535 2254 dbSNP
rs1207306083 2259 dbSNP
rs539253363 2262 dbSNP
rs1274413367 2265 dbSNP
rs1226590681 2268 dbSNP
rs1384566517 2273 dbSNP
rs1313066280 2279 dbSNP
rs57042864 2286 dbSNP
rs1376399282 2294 dbSNP
rs1395072785 2294 dbSNP
rs1299351773 2295 dbSNP
rs1430365043 2299 dbSNP
rs1369819719 2300 dbSNP
rs1166496923 2302 dbSNP
rs549783918 2308 dbSNP
rs755160010 2321 dbSNP
rs1368827614 2324 dbSNP
rs782668586 2326 dbSNP
rs1188580499 2327 dbSNP
rs1423442375 2327 dbSNP
rs1254219621 2330 dbSNP
rs536091325 2333 dbSNP
rs538531286 2341 dbSNP
rs781302680 2342 dbSNP
rs1203466002 2348 dbSNP
rs1362106482 2353 dbSNP
rs144197876 2359 dbSNP
rs748498936 2369 dbSNP
rs1243660263 2371 dbSNP
rs1319507576 2380 dbSNP
rs1308542445 2399 dbSNP
rs782173592 2400 dbSNP
rs1409840916 2401 dbSNP
rs578130132 2403 dbSNP
rs1306168599 2407 dbSNP
rs1406691510 2412 dbSNP
rs1391237308 2429 dbSNP
rs1162409024 2432 dbSNP
rs1457612750 2439 dbSNP
rs1366893623 2459 dbSNP
rs1161463956 2460 dbSNP
rs1472923864 2479 dbSNP
rs1251170339 2482 dbSNP
rs1180704988 2485 dbSNP
rs1453814238 2489 dbSNP
rs1268525649 2490 dbSNP
rs1209577301 2504 dbSNP
rs1487304770 2507 dbSNP
rs1269564965 2512 dbSNP
rs1228608220 2514 dbSNP
rs45529532 2520 dbSNP
rs1285873578 2522 dbSNP
rs1225326437 2523 dbSNP
rs1331558884 2528 dbSNP
rs1322684066 2551 dbSNP
rs1384792471 2552 dbSNP
rs554260357 2566 dbSNP
rs1322868539 2576 dbSNP
rs1457941302 2585 dbSNP
rs1411394699 2586 dbSNP
rs1156975428 2599 dbSNP
rs1479309474 2607 dbSNP
rs782772005 2622 dbSNP
rs1198614655 2629 dbSNP
rs1431859380 2641 dbSNP
rs573775082 2643 dbSNP
rs1250284692 2644 dbSNP
rs572631495 2645 dbSNP
rs1464144119 2648 dbSNP
rs1265720220 2649 dbSNP
rs1201629380 2660 dbSNP
rs1345630689 2660 dbSNP
rs1274113479 2668 dbSNP
rs1230953685 2671 dbSNP
rs1367150387 2672 dbSNP
rs1436121323 2698 dbSNP
rs1388134483 2701 dbSNP
rs778312518 2702 dbSNP
rs1318629730 2703 dbSNP
rs1416189674 2726 dbSNP
rs182842501 2728 dbSNP
rs562531878 2730 dbSNP
rs1172574382 2739 dbSNP
rs1477308161 2741 dbSNP
rs1392469901 2751 dbSNP
rs1189557374 2772 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions TZM-bl
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM1462574. RNA binding protein: AGO2. Condition:TZM-bl ami BaL ...

- Whisnant AW; Bogerd HP; Flores O; Ho P; et al., 2013, mBio.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' guacUAACA-GUGAGAGGUCUcau 5'
              | |||  |||  ||| |   
Target 5' --auACUGUGAACUAACCAAAcca 3'
 		1 - 22
Article - Whisnant AW; Bogerd HP; Flores O; Ho P; et al.
- mBio, 2013
UNLABELLED: The question of how HIV-1 interfaces with cellular microRNA (miRNA) biogenesis and effector mechanisms has been highly controversial. Here, we first used deep sequencing of small RNAs present in two different infected cell lines (TZM-bl and C8166) and two types of primary human cells (CD4(+) peripheral blood mononuclear cells [PBMCs] and macrophages) to unequivocally demonstrate that HIV-1 does not encode any viral miRNAs. Perhaps surprisingly, we also observed that infection of T cells by HIV-1 has only a modest effect on the expression of cellular miRNAs at early times after infection. Comprehensive analysis of miRNA binding to the HIV-1 genome using the photoactivatable ribonucleoside-induced cross-linking and immunoprecipitation (PAR-CLIP) technique revealed several binding sites for cellular miRNAs, a subset of which were shown to be capable of mediating miRNA-mediated repression of gene expression. However, the main finding from this analysis is that HIV-1 transcripts are largely refractory to miRNA binding, most probably due to extensive viral RNA secondary structure. Together, these data demonstrate that HIV-1 neither encodes viral miRNAs nor strongly influences cellular miRNA expression, at least early after infection, and imply that HIV-1 transcripts have evolved to avoid inhibition by preexisting cellular miRNAs by adopting extensive RNA secondary structures that occlude most potential miRNA binding sites. IMPORTANCE: MicroRNAs (miRNAs) are a ubiquitous class of small regulatory RNAs that serve as posttranscriptional regulators of gene expression. Previous work has suggested that HIV-1 might subvert the function of the cellular miRNA machinery by expressing viral miRNAs or by dramatically altering the level of cellular miRNA expression. Using very sensitive approaches, we now demonstrate that neither of these ideas is in fact correct. Moreover, HIV-1 transcripts appear to largely avoid regulation by cellular miRNAs by adopting an extensive RNA secondary structure that occludes the ability of cellular miRNAs to interact with viral mRNAs. Together, these data suggest that HIV-1, rather than seeking to control miRNA function in infected cells, has instead evolved a mechanism to become largely invisible to cellular miRNA effector mechanisms.
LinkOut: [PMID: 23592263]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HCT116
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in ERX177599. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_2_1 PAR-CLIP data was present in ERX177611. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_3_1 ...

- Krell J; Stebbing J; Carissimi C; Dabrowska et al., 2016, Genome research.

Article - Krell J; Stebbing J; Carissimi C; Dabrowska et al.
- Genome research, 2016
DNA damage activates TP53-regulated surveillance mechanisms that are crucial in suppressing tumorigenesis. TP53 orchestrates these responses directly by transcriptionally modulating genes, including microRNAs (miRNAs), and by regulating miRNA biogenesis through interacting with the DROSHA complex. However, whether the association between miRNAs and AGO2 is regulated following DNA damage is not yet known. Here, we show that, following DNA damage, TP53 interacts with AGO2 to induce or reduce AGO2's association of a subset of miRNAs, including multiple let-7 family members. Furthermore, we show that specific mutations in TP53 decrease rather than increase the association of let-7 family miRNAs, reducing their activity without preventing TP53 from interacting with AGO2. This is consistent with the oncogenic properties of these mutants. Using AGO2 RIP-seq and PAR-CLIP-seq, we show that the DNA damage-induced increase in binding of let-7 family members to the RISC complex is functional. We unambiguously determine the global miRNA-mRNA interaction networks involved in the DNA damage response, validating them through the identification of miRNA-target chimeras formed by endogenous ligation reactions. We find that the target complementary region of the let-7 seed tends to have highly fixed positions and more variable ones. Additionally, we observe that miRNAs, whose cellular abundance or differential association with AGO2 is regulated by TP53, are involved in an intricate network of regulatory feedback and feedforward circuits. TP53-mediated regulation of AGO2-miRNA interaction represents a new mechanism of miRNA regulation in carcinogenesis.
LinkOut: [PMID: 26701625]
CLIP-seq Support 1 for dataset GSM1462574
Method / RBP PAR-CLIP / AGO2
Cell line / Condition TZM-bl / TZM-bl ami BaL
Location of target site ENST00000252108.3 | 3UTR | AUACUGUGAACUAACCAAACCAAAACCAACCCCAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23592263 / GSE59944
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
54 hsa-miR-514a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT109577 KLHL15 kelch like family member 15 2 2
MIRT441831 ALG14 ALG14, UDP-N-acetylglucosaminyltransferase subunit 2 2
MIRT442362 UHRF1BP1L UHRF1 binding protein 1 like 2 2
MIRT446171 C8A complement C8 alpha chain 2 2
MIRT463212 ZNF148 zinc finger protein 148 2 2
MIRT464637 UBE4A ubiquitination factor E4A 2 2
MIRT490619 SLC47A1 solute carrier family 47 member 1 2 4
MIRT491282 DHX40 DEAH-box helicase 40 2 2
MIRT502074 KRAS KRAS proto-oncogene, GTPase 2 2
MIRT504057 TOMM5 translocase of outer mitochondrial membrane 5 2 2
MIRT529231 CYP20A1 cytochrome P450 family 20 subfamily A member 1 2 2
MIRT530918 SAP30L SAP30 like 2 2
MIRT539010 AVL9 AVL9 cell migration associated 2 2
MIRT544654 PHF8 PHD finger protein 8 2 4
MIRT548487 EEF2 eukaryotic translation elongation factor 2 2 2
MIRT566598 NUFIP2 NUFIP2, FMR1 interacting protein 2 2 2
MIRT566738 MSANTD4 Myb/SANT DNA binding domain containing 4 with coiled-coils 2 2
MIRT575151 Reep5 receptor accessory protein 5 2 3
MIRT607506 REEP5 receptor accessory protein 5 2 3
MIRT609630 TRPC4AP transient receptor potential cation channel subfamily C member 4 associated protein 2 2
MIRT625605 KLHL23 kelch like family member 23 2 2
MIRT626188 SRFBP1 serum response factor binding protein 1 2 2
MIRT626731 TRIM65 tripartite motif containing 65 2 2
MIRT630313 PDP2 pyruvate dehyrogenase phosphatase catalytic subunit 2 2 2
MIRT631673 VMAC vimentin type intermediate filament associated coiled-coil protein 2 2
MIRT632217 ZFP62 ZFP62 zinc finger protein 2 2
MIRT633969 GRWD1 glutamate rich WD repeat containing 1 2 2
MIRT636971 YY2 YY2 transcription factor 2 2
MIRT637341 DARS2 aspartyl-tRNA synthetase 2, mitochondrial 2 4
MIRT637393 R3HDM2 R3H domain containing 2 2 2
MIRT650380 MOCS3 molybdenum cofactor synthesis 3 2 2
MIRT662553 MTAP methylthioadenosine phosphorylase 2 2
MIRT664112 FUT1 fucosyltransferase 1 (H blood group) 2 2
MIRT664478 ZYG11B zyg-11 family member B, cell cycle regulator 2 2
MIRT669679 ACAP2 ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 2 2
MIRT669913 KIAA0754 KIAA0754 2 4
MIRT670374 ULBP3 UL16 binding protein 3 2 4
MIRT670648 BVES blood vessel epicardial substance 2 2
MIRT670676 KIAA1551 KIAA1551 2 2
MIRT670758 HOOK3 hook microtubule tethering protein 3 2 2
MIRT671325 ACTR1A ARP1 actin related protein 1 homolog A 2 2
MIRT672023 PXMP4 peroxisomal membrane protein 4 2 2
MIRT675326 TBL2 transducin beta like 2 2 2
MIRT675912 C17orf85 nuclear cap binding subunit 3 2 2
MIRT684884 P4HB prolyl 4-hydroxylase subunit beta 2 2
MIRT685291 KIAA1143 KIAA1143 2 2
MIRT691639 CDK1 cyclin dependent kinase 1 2 2
MIRT703936 EPG5 ectopic P-granules autophagy protein 5 homolog 2 2
MIRT713837 NUP98 nucleoporin 98 2 2
MIRT714334 DIP2A disco interacting protein 2 homolog A 2 2
MIRT717948 TUBD1 tubulin delta 1 2 2
MIRT718374 RBM8A RNA binding motif protein 8A 2 2
MIRT719945 SNX8 sorting nexin 8 2 2
MIRT725629 CAMKV CaM kinase like vesicle associated 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-514a-5p Trastuzumab sensitive High Breast Cancer cell line (SKBR3)
hsa-miR-514a-5p Gefitinib 123631 NSC715055 approved sensitive cell line (PC9)
hsa-miR-514a-5p Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-514a-5p Osimertinib 71496458 NSC779217 approved sensitive cell line (PC9)

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