pre-miRNA Information
pre-miRNA hsa-mir-3915   
Genomic Coordinates chrX: 32583656 - 32583752
Description Homo sapiens miR-3915 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-3915
Sequence 21| UUGAGGAAAAGAUGGUCUUAUU |42
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs997323565 10 dbSNP
rs1192161547 15 dbSNP
rs1255075246 19 dbSNP
rs1452812574 20 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol GBA2   
Synonyms AD035, NLGase, SPG46
Description glucosylceramidase beta 2
Transcript NM_020944   
Expression
Putative miRNA Targets on GBA2
3'UTR of GBA2
(miRNA target sites are highlighted)
>GBA2|NM_020944|3'UTR
   1 GCCGTCTGAACTGTGGGAGGGAAGTGCTAACAGCCCAGCCTCCAGCCTGGCCTTTCCTCCTTCCCCTCTGAACCTCCTGC
  81 AACCCTGAGCCATCAGGACAATCATACCCCTTCCCTTCTCTCCACCCAATTGTGCCAGTAAATGGGGGTTGAGGGTGACC
 161 TAGGCAGCATTAGAATCACTTATTTATTTCTTTCCTCACCTGTTCCCTGACTGCGTGAAATGTTCAGGGAGGTCAGTTGA
 241 TTTCCCCAGGTACATTCATGGTGTGACAGACACATGGGTACAAATAAAAGACCCAGAAAGCCAAAAAAAAAAAAAAAAAA
 321 AAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' uuauucuGGUAGA-AAAGGAGUu 5'
                 ::||:| |||||||| 
Target 5' cacttatTTATTTCTTTCCTCAc 3'
177 - 199 155.00 -9.60
2
miRNA  3' uuauUCUGGUAGAAAAGGAGuu 5'
              || |:: | |||||||  
Target 5' ctccAGCCTGGCCTTTCCTCct 3'
40 - 61 138.00 -9.60
3
miRNA  3' uuaUUCUGGUAGA--AAAGGAGUu 5'
             :||::|| :|  ||||| || 
Target 5' aggGAGGTCAGTTGATTTCCCCAg 3'
226 - 249 129.00 -12.60
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN31562514 4 COSMIC
COSN30473541 65 COSMIC
COSN20076936 90 COSMIC
COSN15761822 106 COSMIC
COSN8520559 141 COSMIC
COSN28185107 143 COSMIC
COSN31605212 180 COSMIC
COSN30158367 269 COSMIC
COSN31611318 270 COSMIC
COSN30502016 300 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs186702075 1 dbSNP
rs747837125 3 dbSNP
rs1408408323 4 dbSNP
rs781470140 6 dbSNP
rs780899461 11 dbSNP
rs1233912055 12 dbSNP
rs1244965342 13 dbSNP
rs732784 14 dbSNP
rs372562411 17 dbSNP
rs1336568849 19 dbSNP
rs767673780 25 dbSNP
rs1245774556 26 dbSNP
rs1217454341 27 dbSNP
rs751208161 32 dbSNP
rs766034702 39 dbSNP
rs757950539 41 dbSNP
rs1357836094 46 dbSNP
rs953706983 49 dbSNP
rs1389261627 56 dbSNP
rs1234923409 57 dbSNP
rs1470701508 58 dbSNP
rs1177041717 63 dbSNP
rs1410019890 64 dbSNP
rs1003010507 67 dbSNP
rs1167565082 68 dbSNP
rs920969258 95 dbSNP
rs1403492428 101 dbSNP
rs1330773059 103 dbSNP
rs1355091079 106 dbSNP
rs762150608 107 dbSNP
rs1475862579 116 dbSNP
rs1286297960 120 dbSNP
rs546341042 124 dbSNP
rs971516173 125 dbSNP
rs974334885 126 dbSNP
rs963320399 129 dbSNP
rs112785437 130 dbSNP
rs764517257 141 dbSNP
rs971556515 143 dbSNP
rs1204799979 148 dbSNP
rs1250078722 149 dbSNP
rs13287359 160 dbSNP
rs1213572258 161 dbSNP
rs1486745944 163 dbSNP
rs1049100408 167 dbSNP
rs115645052 174 dbSNP
rs1013037877 178 dbSNP
rs528287633 183 dbSNP
rs1471838253 184 dbSNP
rs1169783858 186 dbSNP
rs1420134821 186 dbSNP
rs182108271 186 dbSNP
rs111496988 187 dbSNP
rs1037332661 190 dbSNP
rs768898931 190 dbSNP
rs532364442 192 dbSNP
rs560639241 197 dbSNP
rs903739878 207 dbSNP
rs1341005657 209 dbSNP
rs1042325861 212 dbSNP
rs945169716 214 dbSNP
rs932426169 215 dbSNP
rs914269576 231 dbSNP
rs912430042 237 dbSNP
rs1295073258 238 dbSNP
rs1340602853 239 dbSNP
rs1228340482 254 dbSNP
rs1050798984 259 dbSNP
rs1350459459 260 dbSNP
rs1212108144 265 dbSNP
rs1255720313 269 dbSNP
rs955871413 275 dbSNP
rs932425580 276 dbSNP
rs775952951 277 dbSNP
rs1242791326 278 dbSNP
rs770501648 286 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions TZM-bl
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM1462574. RNA binding protein: AGO2. Condition:TZM-bl ami BaL ...

- Whisnant AW; Bogerd HP; Flores O; Ho P; et al., 2013, mBio.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' uuauucuGGUAGA-AAAGGAGUu 5'
                 ::||:| |||||||| 
Target 5' cacuuauUUAUUUCUUUCCUCAc 3'
4 - 26
Article - Whisnant AW; Bogerd HP; Flores O; Ho P; et al.
- mBio, 2013
UNLABELLED: The question of how HIV-1 interfaces with cellular microRNA (miRNA) biogenesis and effector mechanisms has been highly controversial. Here, we first used deep sequencing of small RNAs present in two different infected cell lines (TZM-bl and C8166) and two types of primary human cells (CD4(+) peripheral blood mononuclear cells [PBMCs] and macrophages) to unequivocally demonstrate that HIV-1 does not encode any viral miRNAs. Perhaps surprisingly, we also observed that infection of T cells by HIV-1 has only a modest effect on the expression of cellular miRNAs at early times after infection. Comprehensive analysis of miRNA binding to the HIV-1 genome using the photoactivatable ribonucleoside-induced cross-linking and immunoprecipitation (PAR-CLIP) technique revealed several binding sites for cellular miRNAs, a subset of which were shown to be capable of mediating miRNA-mediated repression of gene expression. However, the main finding from this analysis is that HIV-1 transcripts are largely refractory to miRNA binding, most probably due to extensive viral RNA secondary structure. Together, these data demonstrate that HIV-1 neither encodes viral miRNAs nor strongly influences cellular miRNA expression, at least early after infection, and imply that HIV-1 transcripts have evolved to avoid inhibition by preexisting cellular miRNAs by adopting extensive RNA secondary structures that occlude most potential miRNA binding sites. IMPORTANCE: MicroRNAs (miRNAs) are a ubiquitous class of small regulatory RNAs that serve as posttranscriptional regulators of gene expression. Previous work has suggested that HIV-1 might subvert the function of the cellular miRNA machinery by expressing viral miRNAs or by dramatically altering the level of cellular miRNA expression. Using very sensitive approaches, we now demonstrate that neither of these ideas is in fact correct. Moreover, HIV-1 transcripts appear to largely avoid regulation by cellular miRNAs by adopting an extensive RNA secondary structure that occludes the ability of cellular miRNAs to interact with viral mRNAs. Together, these data suggest that HIV-1, rather than seeking to control miRNA function in infected cells, has instead evolved a mechanism to become largely invisible to cellular miRNA effector mechanisms.
LinkOut: [PMID: 23592263]
CLIP-seq Support 1 for dataset GSM4903826
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / PID21_NS
Location of target site NM_020944 | 3UTR | ACCUGUUCCCUGACUGCGUGAAAUGUUCAGGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161237
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4903829
Method / RBP HITS-CLIP / AGO
Cell line / Condition Human neurons / CTLTD_shCTL_a
Location of target site NM_020944 | 3UTR | CUUAUUUAUUUCUUUCCUCACCUGUUCCCUGACUGCGUGAAAUGUUCAGGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161238
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM4903833
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / CTL_TD_21_a
Location of target site NM_020944 | 3UTR | UCACCUGUUCCCUGACUGCGUGAAAUGUUCAGGGAGGUCAGUUGAUUUCCCCAGGUACAUUCAUGGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM4903835
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / CTL_TD_21_c
Location of target site NM_020944 | 3UTR | CCUAGGCAGCAUUAGAAUCACUUAUUUAUUUCUUUCCUCACCUGUUCCCUGACUGCGUGAAAUGUUCAGGGA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM4903837
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_b
Location of target site NM_020944 | 3UTR | AGGCAGCAUUAGAAUCACUUAUUUAUUUCUUUCCUCACCUGUUCCCUGACUGCGUGAAAUGUUCAGGGAGGUCAGUUGAUUUCCCCAGGUACAUUCAUGGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset GSM1462574
Method / RBP PAR-CLIP / AGO2
Cell line / Condition TZM-bl / TZM-bl ami BaL
Location of target site ENST00000378103.3 | 3UTR | AAUCACUUAUUUAUUUCUUUCCUCACCUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23592263 / GSE59944
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
111 hsa-miR-3915 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT059544 PIP5K1A phosphatidylinositol-4-phosphate 5-kinase type 1 alpha 2 2
MIRT081703 ZNF507 zinc finger protein 507 2 2
MIRT083311 ZCCHC3 zinc finger CCHC-type containing 3 2 6
MIRT119046 SFT2D3 SFT2 domain containing 3 2 2
MIRT189381 TXLNA taxilin alpha 2 4
MIRT195895 C16ORF72 chromosome 16 open reading frame 72 2 6
MIRT223807 OXR1 oxidation resistance 1 2 2
MIRT292954 ZNF146 zinc finger protein 146 2 4
MIRT293949 RPL13A ribosomal protein L13a 2 6
MIRT300900 KREMEN1 kringle containing transmembrane protein 1 2 2
MIRT339332 SESN2 sestrin 2 2 2
MIRT349304 ZNF317 zinc finger protein 317 2 2
MIRT364736 TOR1B torsin family 1 member B 2 2
MIRT366233 VMA21 VMA21, vacuolar ATPase assembly factor 2 2
MIRT384605 CLIC4 chloride intracellular channel 4 2 2
MIRT401745 HLA-DRA major histocompatibility complex, class II, DR alpha 2 2
MIRT443169 UBL3 ubiquitin like 3 2 2
MIRT444215 METTL12 methyltransferase like 12 2 2
MIRT444375 SH3TC2 SH3 domain and tetratricopeptide repeats 2 2 2
MIRT445340 TCEANC transcription elongation factor A N-terminal and central domain containing 2 2
MIRT445474 KDM6A lysine demethylase 6A 2 2
MIRT445598 CAMK2N1 calcium/calmodulin dependent protein kinase II inhibitor 1 2 2
MIRT445630 TMEM50A transmembrane protein 50A 2 2
MIRT446012 VNN1 vanin 1 2 2
MIRT446115 ASTN1 astrotactin 1 2 2
MIRT446248 ELP2 elongator acetyltransferase complex subunit 2 2 2
MIRT446382 SYNCRIP synaptotagmin binding cytoplasmic RNA interacting protein 2 2
MIRT446940 ZMAT3 zinc finger matrin-type 3 2 2
MIRT446967 SLCO4C1 solute carrier organic anion transporter family member 4C1 2 2
MIRT447178 PGRMC2 progesterone receptor membrane component 2 2 2
MIRT447209 APBB2 amyloid beta precursor protein binding family B member 2 2 2
MIRT447237 IHH indian hedgehog 2 2
MIRT447807 EMX1 empty spiracles homeobox 1 2 2
MIRT447853 RRP8 ribosomal RNA processing 8 2 4
MIRT448054 MMP15 matrix metallopeptidase 15 2 2
MIRT448094 RASD2 RASD family member 2 2 2
MIRT448704 KLHL11 kelch like family member 11 2 2
MIRT448851 FEM1C fem-1 homolog C 2 2
MIRT449488 ZBTB4 zinc finger and BTB domain containing 4 2 2
MIRT449785 C1orf109 chromosome 1 open reading frame 109 2 2
MIRT450774 PDE3A phosphodiesterase 3A 2 2
MIRT451124 ZNF99 zinc finger protein 99 2 2
MIRT452676 GPR156 G protein-coupled receptor 156 2 2
MIRT452888 PSD4 pleckstrin and Sec7 domain containing 4 2 2
MIRT453188 ACSF2 acyl-CoA synthetase family member 2 2 2
MIRT453353 ZNF3 zinc finger protein 3 2 2
MIRT454502 ZFYVE27 zinc finger FYVE-type containing 27 2 2
MIRT454629 FAM83H family with sequence similarity 83 member H 2 2
MIRT455183 AGTRAP angiotensin II receptor associated protein 2 2
MIRT458204 FOXL2 forkhead box L2 2 2
MIRT458723 CES2 carboxylesterase 2 2 2
MIRT458942 SAMD4B sterile alpha motif domain containing 4B 2 2
MIRT460304 FLCN folliculin 2 2
MIRT460989 SYT7 synaptotagmin 7 2 2
MIRT461697 ZNF426 zinc finger protein 426 2 2
MIRT461903 NECAB3 N-terminal EF-hand calcium binding protein 3 2 2
MIRT462193 NDUFS1 NADH:ubiquinone oxidoreductase core subunit S1 2 2
MIRT462290 PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H 2 2
MIRT463795 XPOT exportin for tRNA 2 2
MIRT464649 UBE2V1 ubiquitin conjugating enzyme E2 V1 2 4
MIRT465911 TMEM189-UBE2V1 TMEM189-UBE2V1 readthrough 2 4
MIRT465992 TMEM189 transmembrane protein 189 2 4
MIRT466302 TIMM22 translocase of inner mitochondrial membrane 22 2 2
MIRT466575 TBC1D2B TBC1 domain family member 2B 2 2
MIRT470558 POU2F1 POU class 2 homeobox 1 2 2
MIRT471333 PERP PERP, TP53 apoptosis effector 2 2
MIRT471659 PALM2 paralemmin 2 2 2
MIRT472363 TSPAN1 tetraspanin 1 2 2
MIRT473468 MCFD2 multiple coagulation factor deficiency 2 2 2
MIRT474702 KIF3A kinesin family member 3A 2 2
MIRT476067 GRIN2A glutamate ionotropic receptor NMDA type subunit 2A 2 2
MIRT476077 GRB2 growth factor receptor bound protein 2 2 2
MIRT476423 GBA2 glucosylceramidase beta 2 2 2
MIRT476463 GATAD2B GATA zinc finger domain containing 2B 2 2
MIRT477877 DYNLL2 dynein light chain LC8-type 2 2 2
MIRT478240 DDX3X DEAD-box helicase 3, X-linked 2 4
MIRT479460 CDK6 cyclin dependent kinase 6 2 2
MIRT481030 BAZ2A bromodomain adjacent to zinc finger domain 2A 2 2
MIRT491496 HLA-DOA major histocompatibility complex, class II, DO alpha 2 2
MIRT497585 SLC23A1 solute carrier family 23 member 1 2 2
MIRT498542 TMEM30B transmembrane protein 30B 2 2
MIRT499263 NBPF11 NBPF member 11 2 2
MIRT504698 ZNF117 zinc finger protein 117 2 2
MIRT511288 KLHL15 kelch like family member 15 2 4
MIRT523121 HSP90B1 heat shock protein 90 beta family member 1 2 4
MIRT529601 H1F0 H1 histone family member 0 2 2
MIRT533481 TRIM71 tripartite motif containing 71 2 2
MIRT535571 NUP37 nucleoporin 37 2 4
MIRT537869 EDA2R ectodysplasin A2 receptor 2 2
MIRT538010 DNAJC10 DnaJ heat shock protein family (Hsp40) member C10 2 2
MIRT552681 YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta 2 4
MIRT554986 RAB39B RAB39B, member RAS oncogene family 2 2
MIRT556659 KMT2D lysine methyltransferase 2D 2 4
MIRT557437 GTPBP2 GTP binding protein 2 2 2
MIRT560813 CRTAP cartilage associated protein 2 2
MIRT562115 IGFBP5 insulin like growth factor binding protein 5 2 2
MIRT563103 IFRD2 interferon related developmental regulator 2 2 2
MIRT574199 LMNB1 lamin B1 2 2
MIRT623920 FMNL3 formin like 3 2 2
MIRT626551 NMNAT2 nicotinamide nucleotide adenylyltransferase 2 2 2
MIRT645458 ANKS6 ankyrin repeat and sterile alpha motif domain containing 6 2 2
MIRT649082 CACNA1B calcium voltage-gated channel subunit alpha1 B 2 2
MIRT659477 CLDN1 claudin 1 2 2
MIRT667950 HMGCS1 3-hydroxy-3-methylglutaryl-CoA synthase 1 2 2
MIRT701981 MIER3 MIER family member 3 2 2
MIRT708694 TFDP2 transcription factor Dp-2 2 2
MIRT709649 DFFB DNA fragmentation factor subunit beta 2 2
MIRT710090 FAM229B family with sequence similarity 229 member B 2 2
MIRT718396 ALDH1A3 aldehyde dehydrogenase 1 family member A3 2 2
MIRT724972 TNS1 tensin 1 2 2
MIRT756050 HRH4 histamine receptor H4 2 1
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-3915 Paclitaxel 36314 NSC125973 approved resistant High Prostate Cancer cell line (DU-145, PC-3)
hsa-miR-3915 Cisplatin + Decitabine sensitive High Non-Small Cell Lung Cancer cell line (A549)
hsa-mir-3915 Cisplatin 5460033 NSC119875 approved sensitive cell line (BxPC3)
hsa-miR-3915 Tripterygium wilfordii Hook F sensitive tissue
hsa-miR-3915 Cisplatin 5460033 NSC119875 approved resistant cell line (A549)
hsa-miR-3915 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-3915 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)

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