pre-miRNA Information
pre-miRNA hsa-mir-3179-1   
Genomic Coordinates chr16: 14901508 - 14901591
Description Homo sapiens miR-3179-1 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases
pre-miRNA hsa-mir-3179-2   
Genomic Coordinates chr16: 16300159 - 16300242
Description Homo sapiens miR-3179-2 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases
pre-miRNA hsa-mir-3179-3   
Genomic Coordinates chr16: 18411894 - 18411977
Description Homo sapiens miR-3179-3 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases
pre-miRNA hsa-mir-3179-4   
Genomic Coordinates chr16: 18494493 - 18494576
Description Homo sapiens miR-3179-4 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-3179
Sequence 52| AGAAGGGGUGAAAUUUAAACGU |73
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1351100204 1 dbSNP
rs1398613616 4 dbSNP
rs1340262765 20 dbSNP
rs1229893475 21 dbSNP
Putative Targets

miRNA Expression profile
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol RNF11   
Synonyms CGI-123, SID1669
Description ring finger protein 11
Transcript NM_014372   
Expression
Putative miRNA Targets on RNF11
3'UTR of RNF11
(miRNA target sites are highlighted)
>RNF11|NM_014372|3'UTR
   1 GCCAGGGTCTCTTATCTGACTTCAAGTGAACCACCATTTTGGTGGTTTTGATCTTTTGTCACTGAGCCCAAAGAGCCAGG
  81 GATTAGGAATTAAGATCGTGCACAAAAGTTTCCTTAAAATTCCTGGATGGCTGCAGATGTTGGGGGAAAAAGTACGTGAT
 161 ATTTTAGAAACTTAGTGGGAAAAGTAGGATGGTATTTTTATGTAAAGCCTTGACCCAATGTTTAAAAATATAATTGTATT
 241 TAGATCTTGTTATTGCTCCAGTACATAGGAATTGTGTAAAGTGTTAACAGCAGCTGTATTTGTTTAAATTGTGTGTATTG
 321 AAGATTAGGAAAAAGATAGTAGTTATTTTTCCTAAATGAAATAACTTTCTTCTCTTCCCCTTCCCCACCCGAATTCTTTT
 401 CTGAAGTTGCTGGCATTTGGGTCAAGGTTTTATTAAAAGCTACATTTTATAACACTGGCACACACAAAAAAGTAGTTTTA
 481 AGCTTGTTTGCACAGTTCTTTTTTTCCATTGGAAATGGAATTCATTGCCTTAGGTCTTTTTAAATAGTGTATTATTATCG
 561 TTGGGGCTGGCTCTATGCTTGAAAACCAGTTTATTTATAACCTGTTATAAGTGCTATATTCTGTTTGCAGTTAGGAAATG
 641 CAGAATTCAAAGTGATCTCCTAGCTTGTAAGCAAACTGAGATGCACTATCCCTTTTCTATAAAAAATAAGTTAATGTGTC
 721 AAGAAACCAACTCTATTAAGGTGGGGTTTAATATTACCCTTTCCTATGTGTTTTATCTAATTATTTTGGTTGTTAATATG
 801 GTGATAATGGAAAGTCAAGTTAAATTTTAAATATTAAGAATTCTGATTTATTGAGATTGAATTATGCCACCACGTTTATG
 881 TAAAAATGAAGGTGGCACCGTGGTGAGACCTAATGAGAAATAGTTACTCAGTTGTAAAAATTTTGATTTATTCTCTTTCT
 961 TCTGACCTCCTTGCCTCTTGTCTTGAACCATAGCAAAAGGATACTGCATCTCTCATTACTGTAGTGCTGAGGTTATTGAA
1041 GTTATACAAAACACATCTCAGTCTCTGTTTCTTGGAAAGGTATCTATTACATCCTGCTAGCTGACTGACAAAACTAAGCA
1121 GGGAGAATAAAGATAATTGTATTTTATGTTTTGCACACAAACGCAGAATTTGTATAACCATATGACTTCATAGTTGTGAT
1201 CTCAAAAAAGAAGGAATTTCTCCTTTGTTTCTTGCAGTTAATGTAAGAATACTTTAAATCTCTAAGCTTCTGAAGTGTTA
1281 GAGGTAGAGATGGTCTAGTAAAGATGTAGTAGTAATGTTTTATCCATTTAGCATGTGTTTATTTTTTCATATGTACTCAA
1361 AGGTGACTTATTGGTTCACCTCAGTGATATTACAGCTAAAAAAATCATTCATTAGCAAAAGGAAAAGTGGTCTCAACCTA
1441 ACATCAGAAGTGTTTCTTATTATTATTTTATATTGAGTTGAATATTGAACTCTAACAGTTTTCTACATACAAAACACAGT
1521 GTCATGAAGGTTATTCATAATTGCATTATAGAGGAATGTAGTATGTCATAAGTACTTTGTAAAGATTTGACATTCAACTG
1601 TAGTATCCATATGTTGCTTAAATTTCCTTATGAGCCCCATGATGGAAAGACTTAAAGATGAATTTGAGAAAAATTGAAAG
1681 AAATTAGATTATCAGGTTCTGTTAAATTGTTACATGTATCTTGCTTAAATTTCTGTTTATTAATTTATATCCACCCAAGT
1761 ACATAAAGCAAATTTGGAGGAAACAACTGAAGTTGTGCAATATTTTCTGATAATTGCTTTTTTTATTCTTGTGTTTTCTA
1841 CTTAAACATAATGTCTGTGTCATCAAGTATTATAGTCAGACTTTTCTTTTTTTCTAGATTGTTAAAATTGGCAAATGAAC
1921 TTTTTTAAAAATCATCTTCCATGTTGCAGTTAGTCTTTCTTTTCATTACAAGTCTTTCACAGAAGTTTGGTGGTAATATT
2001 GAAAGAACTAGCATTGGGCAGAATGTGTCTTTTTTAGGCACTTTATATTCTCAACATACAATGTTAAGAACCATCAATTT
2081 TGACTTTTACTAAGTTGTTAAATAAAGTTATAATACAGCTGTGAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' ugCAAAUUUAAAGUGGGGAAGa 5'
            |||| ||| |:||||:||| 
Target 5' ggGTTT-AATATTACCCTTTCc 3'
744 - 764 159.00 -16.00
2
miRNA  3' ugcaaAUUUAAAG------UGGGGAAGa 5'
               ||| ||||       ||||||| 
Target 5' tgaaaTAACTTTCTTCTCTTCCCCTTCc 3'
357 - 384 151.00 -11.60
3
miRNA  3' ugcAAAUUUAAAGUGGGGAAGa 5'
             |||:| || : |:|:||| 
Target 5' aatTTTGATTTATTCTCTTTCt 3'
939 - 960 131.00 -9.20
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN16136976 17 COSMIC
COSN1105486 24 COSMIC
COSN8638430 64 COSMIC
COSN31530958 77 COSMIC
COSN13550254 98 COSMIC
COSN20073407 99 COSMIC
COSN30125252 102 COSMIC
COSN16129099 143 COSMIC
COSN28867239 152 COSMIC
COSN31550907 175 COSMIC
COSN30135771 191 COSMIC
COSN31546778 373 COSMIC
COSN30542057 410 COSMIC
COSN31578677 673 COSMIC
COSN31603437 855 COSMIC
COSN22216894 857 COSMIC
COSN31548819 892 COSMIC
COSN31577314 917 COSMIC
COSN31541232 925 COSMIC
COSN31533621 1032 COSMIC
COSN31540496 1131 COSMIC
COSN31513709 1171 COSMIC
COSN21871698 1282 COSMIC
COSN16623637 1537 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs750945149 1 dbSNP
rs1157833864 2 dbSNP
rs1388897084 4 dbSNP
rs1381988459 5 dbSNP
rs1268065026 7 dbSNP
rs1429515882 8 dbSNP
rs1383627599 13 dbSNP
rs1441759857 14 dbSNP
rs756661289 15 dbSNP
rs374679731 16 dbSNP
rs752194608 22 dbSNP
rs1304678200 24 dbSNP
rs758126066 26 dbSNP
rs1407466727 36 dbSNP
rs777353077 43 dbSNP
rs751206505 46 dbSNP
rs775914972 51 dbSNP
rs1443710653 57 dbSNP
rs947307962 69 dbSNP
rs1417386064 76 dbSNP
rs76909946 82 dbSNP
rs565470362 95 dbSNP
rs1197760992 96 dbSNP
rs1374638850 98 dbSNP
rs1490288228 99 dbSNP
rs1276052330 102 dbSNP
rs61100121 108 dbSNP
rs1308756685 109 dbSNP
rs1270226284 120 dbSNP
rs1167300546 131 dbSNP
rs564808037 134 dbSNP
rs1213651835 140 dbSNP
rs563193580 148 dbSNP
rs768966978 152 dbSNP
rs1402932818 153 dbSNP
rs774546077 156 dbSNP
rs886196224 157 dbSNP
rs1005049575 168 dbSNP
rs1352856171 169 dbSNP
rs1169883545 172 dbSNP
rs1415076038 176 dbSNP
rs1431802418 193 dbSNP
rs1016868184 219 dbSNP
rs1480323583 231 dbSNP
rs1404922490 232 dbSNP
rs1435441928 233 dbSNP
rs1252910597 236 dbSNP
rs1179499809 243 dbSNP
rs530090663 258 dbSNP
rs1325093364 262 dbSNP
rs963585015 266 dbSNP
rs1232788007 269 dbSNP
rs1203260527 275 dbSNP
rs533326693 277 dbSNP
rs767062438 284 dbSNP
rs1227314955 292 dbSNP
rs772845556 299 dbSNP
rs764524727 300 dbSNP
rs1264629414 311 dbSNP
rs566788322 314 dbSNP
rs868770595 315 dbSNP
rs954673121 319 dbSNP
rs1307381837 327 dbSNP
rs1371141018 328 dbSNP
rs1297531454 336 dbSNP
rs1347166146 337 dbSNP
rs1221770284 338 dbSNP
rs1448926511 342 dbSNP
rs189514417 353 dbSNP
rs1334978670 373 dbSNP
rs367629850 378 dbSNP
rs1465502482 386 dbSNP
rs552438992 391 dbSNP
rs1263546608 397 dbSNP
rs568462622 405 dbSNP
rs1445113389 413 dbSNP
rs957044313 418 dbSNP
rs1378162276 422 dbSNP
rs988641734 426 dbSNP
rs914435228 428 dbSNP
rs370652065 445 dbSNP
rs1201411849 448 dbSNP
rs77924765 453 dbSNP
rs1044311706 459 dbSNP
rs1217759260 460 dbSNP
rs1314893445 466 dbSNP
rs1306221522 467 dbSNP
rs537664477 484 dbSNP
rs1246542984 502 dbSNP
rs11553363 508 dbSNP
rs149441148 510 dbSNP
rs1457418316 523 dbSNP
rs1312957772 525 dbSNP
rs1451979272 526 dbSNP
rs1385999786 528 dbSNP
rs1290852691 532 dbSNP
rs1452158972 533 dbSNP
rs927196240 537 dbSNP
rs564796277 542 dbSNP
rs56890493 548 dbSNP
rs886172458 551 dbSNP
rs1181254928 554 dbSNP
rs530812754 555 dbSNP
rs1262060794 557 dbSNP
rs1187851089 560 dbSNP
rs1484775247 561 dbSNP
rs1259892487 566 dbSNP
rs1037427988 568 dbSNP
rs899317805 576 dbSNP
rs1289467207 585 dbSNP
rs1245425259 588 dbSNP
rs1324709994 590 dbSNP
rs1327088875 593 dbSNP
rs1333345004 594 dbSNP
rs1380703738 599 dbSNP
rs1361724952 609 dbSNP
rs1426906185 617 dbSNP
rs1364321281 618 dbSNP
rs1163487187 625 dbSNP
rs1423464436 626 dbSNP
rs1403698590 627 dbSNP
rs1172132482 629 dbSNP
rs1282720884 638 dbSNP
rs1329973261 639 dbSNP
rs1230433161 660 dbSNP
rs7340123 661 dbSNP
rs1199932445 662 dbSNP
rs1259204027 666 dbSNP
rs1490778479 668 dbSNP
rs535418064 674 dbSNP
rs1221580919 684 dbSNP
rs553716996 687 dbSNP
rs369089634 689 dbSNP
rs1253102185 690 dbSNP
rs1204333550 698 dbSNP
rs1225306057 701 dbSNP
rs1307175511 706 dbSNP
rs1284154164 707 dbSNP
rs996305546 717 dbSNP
rs1343960022 731 dbSNP
rs1303087152 733 dbSNP
rs1267873045 736 dbSNP
rs1029169102 741 dbSNP
rs182062668 746 dbSNP
rs1490819777 759 dbSNP
rs545834885 760 dbSNP
rs760194952 767 dbSNP
rs1031441583 768 dbSNP
rs1472646091 772 dbSNP
rs1160791442 776 dbSNP
rs956798078 778 dbSNP
rs1198628360 780 dbSNP
rs1438373616 783 dbSNP
rs147498188 788 dbSNP
rs1194215772 791 dbSNP
rs1453424739 792 dbSNP
rs1164184816 793 dbSNP
rs1264000960 800 dbSNP
rs915346020 808 dbSNP
rs376927190 834 dbSNP
rs968499410 838 dbSNP
rs979939780 853 dbSNP
rs1240181862 857 dbSNP
rs1338089171 859 dbSNP
rs766442366 861 dbSNP
rs1367921164 874 dbSNP
rs1423255973 875 dbSNP
rs1303131298 881 dbSNP
rs1351123991 895 dbSNP
rs1411771846 900 dbSNP
rs138476185 901 dbSNP
rs544842668 904 dbSNP
rs552332541 909 dbSNP
rs938621883 912 dbSNP
rs1265781354 913 dbSNP
rs563106928 918 dbSNP
rs1056788010 921 dbSNP
rs1220098103 922 dbSNP
rs1418581206 923 dbSNP
rs1243783871 924 dbSNP
rs1205834305 926 dbSNP
rs907504774 937 dbSNP
rs1290987301 949 dbSNP
rs1483060135 964 dbSNP
rs1206163856 969 dbSNP
rs1178446341 970 dbSNP
rs1247875884 976 dbSNP
rs1418143495 978 dbSNP
rs1238881550 981 dbSNP
rs1375545032 991 dbSNP
rs1187865144 993 dbSNP
rs1387447305 994 dbSNP
rs754224012 997 dbSNP
rs1454087146 1001 dbSNP
rs1333079350 1009 dbSNP
rs74849425 1009 dbSNP
rs753787713 1016 dbSNP
rs1386780155 1022 dbSNP
rs1371715798 1024 dbSNP
rs1385762980 1027 dbSNP
rs940389033 1028 dbSNP
rs754936555 1035 dbSNP
rs1237182176 1039 dbSNP
rs1194102661 1040 dbSNP
rs1449104725 1042 dbSNP
rs1296521545 1046 dbSNP
rs1204892942 1048 dbSNP
rs757628551 1053 dbSNP
rs765132505 1064 dbSNP
rs1317461962 1066 dbSNP
rs1247604295 1073 dbSNP
rs1379867171 1076 dbSNP
rs1050595525 1083 dbSNP
rs1430958025 1084 dbSNP
rs1328001607 1087 dbSNP
rs1384675175 1091 dbSNP
rs1399132938 1093 dbSNP
rs890692881 1099 dbSNP
rs1165384489 1100 dbSNP
rs1009590238 1110 dbSNP
rs1413083737 1114 dbSNP
rs1031263990 1137 dbSNP
rs1476539835 1148 dbSNP
rs1206619631 1156 dbSNP
rs143495145 1160 dbSNP
rs34974872 1163 dbSNP
rs558637221 1164 dbSNP
rs576815229 1166 dbSNP
rs1010912840 1168 dbSNP
rs1490610579 1173 dbSNP
rs1022354673 1181 dbSNP
rs1220816687 1184 dbSNP
rs1319436225 1196 dbSNP
rs147171069 1197 dbSNP
rs1200628794 1198 dbSNP
rs1214625180 1207 dbSNP
rs1341087621 1209 dbSNP
rs1268042556 1216 dbSNP
rs1244626371 1223 dbSNP
rs534665791 1233 dbSNP
rs1034213724 1241 dbSNP
rs1277149739 1249 dbSNP
rs959913678 1250 dbSNP
rs993132065 1260 dbSNP
rs1183378906 1261 dbSNP
rs1329666464 1263 dbSNP
rs1411723764 1265 dbSNP
rs1391226243 1280 dbSNP
rs191710775 1284 dbSNP
rs1430783623 1290 dbSNP
rs907789423 1291 dbSNP
rs1169018878 1296 dbSNP
rs1431851360 1298 dbSNP
rs940318067 1304 dbSNP
rs375913847 1306 dbSNP
rs973078646 1306 dbSNP
rs1338869820 1310 dbSNP
rs1439029539 1311 dbSNP
rs1404203819 1316 dbSNP
rs1452265268 1317 dbSNP
rs1217569465 1326 dbSNP
rs11553364 1330 dbSNP
rs552600570 1334 dbSNP
rs1284910128 1341 dbSNP
rs1221429302 1344 dbSNP
rs1050538320 1349 dbSNP
rs1280048917 1364 dbSNP
rs570632964 1367 dbSNP
rs1330968440 1368 dbSNP
rs1216233052 1372 dbSNP
rs531718353 1373 dbSNP
rs1408541561 1380 dbSNP
rs1174777200 1385 dbSNP
rs1432965974 1389 dbSNP
rs549548850 1391 dbSNP
rs1179404881 1397 dbSNP
rs944910177 1398 dbSNP
rs1197651980 1405 dbSNP
rs1223593360 1413 dbSNP
rs1053075696 1417 dbSNP
rs1207824706 1427 dbSNP
rs1311330053 1430 dbSNP
rs1307619626 1439 dbSNP
rs892725290 1444 dbSNP
rs1010902986 1454 dbSNP
rs1264547059 1457 dbSNP
rs1308680708 1457 dbSNP
rs1334956178 1458 dbSNP
rs1465868622 1464 dbSNP
rs748392833 1475 dbSNP
rs769806469 1493 dbSNP
rs1399416460 1506 dbSNP
rs1380207468 1508 dbSNP
rs546959884 1514 dbSNP
rs1244604260 1517 dbSNP
rs1444701382 1517 dbSNP
rs1002260423 1525 dbSNP
rs750646728 1526 dbSNP
rs567952005 1527 dbSNP
rs1185252612 1547 dbSNP
rs1482822687 1552 dbSNP
rs1261044484 1558 dbSNP
rs1351469344 1564 dbSNP
rs1285217573 1570 dbSNP
rs1246881241 1573 dbSNP
rs1361609082 1575 dbSNP
rs1310852900 1579 dbSNP
rs1395631118 1591 dbSNP
rs1035130654 1595 dbSNP
rs745956428 1596 dbSNP
rs960105790 1597 dbSNP
rs992685080 1600 dbSNP
rs1178663579 1602 dbSNP
rs1164706938 1603 dbSNP
rs1015250413 1606 dbSNP
rs1427370772 1607 dbSNP
rs1386514676 1609 dbSNP
rs961976346 1610 dbSNP
rs535734705 1613 dbSNP
rs1160243275 1618 dbSNP
rs1261956902 1623 dbSNP
rs181233941 1627 dbSNP
rs1487179328 1637 dbSNP
rs565627817 1638 dbSNP
rs1223047822 1642 dbSNP
rs1490871026 1644 dbSNP
rs973026767 1646 dbSNP
rs1345515730 1659 dbSNP
rs1308346270 1676 dbSNP
rs1297975170 1686 dbSNP
rs1217196148 1692 dbSNP
rs920287655 1693 dbSNP
rs1304308849 1719 dbSNP
rs1407240919 1739 dbSNP
rs1389175495 1741 dbSNP
rs35241499 1744 dbSNP
rs1291324628 1761 dbSNP
rs553441678 1785 dbSNP
rs931661356 1786 dbSNP
rs1373352116 1787 dbSNP
rs1172015002 1789 dbSNP
rs985818440 1790 dbSNP
rs1422604853 1801 dbSNP
rs1200094303 1810 dbSNP
rs1432673502 1811 dbSNP
rs1490835430 1818 dbSNP
rs1286975072 1825 dbSNP
rs1265148185 1826 dbSNP
rs1361196725 1835 dbSNP
rs1405541443 1842 dbSNP
rs1480966040 1843 dbSNP
rs1253296951 1850 dbSNP
rs1196935178 1851 dbSNP
rs1317709488 1854 dbSNP
rs911649876 1860 dbSNP
rs1320864281 1868 dbSNP
rs758585865 1872 dbSNP
rs568182753 1874 dbSNP
rs756624047 1878 dbSNP
rs1332989745 1881 dbSNP
rs1463691572 1882 dbSNP
rs1399073952 1888 dbSNP
rs1214106033 1893 dbSNP
rs1176943627 1900 dbSNP
rs557860963 1912 dbSNP
rs1375869716 1916 dbSNP
rs1241958833 1917 dbSNP
rs576187216 1927 dbSNP
rs1044014012 1930 dbSNP
rs1176830809 1931 dbSNP
rs1202274083 1934 dbSNP
rs896531887 1936 dbSNP
rs1266834220 1944 dbSNP
rs1263985993 1948 dbSNP
rs1480163305 1954 dbSNP
rs1183116629 1973 dbSNP
rs748359285 1975 dbSNP
rs1410439407 1986 dbSNP
rs1402167338 1987 dbSNP
rs905169584 1987 dbSNP
rs1156649480 1988 dbSNP
rs1240342695 1989 dbSNP
rs1002206413 1991 dbSNP
rs949350884 1999 dbSNP
rs1443899649 2002 dbSNP
rs1375784528 2003 dbSNP
rs1336970739 2009 dbSNP
rs1045629662 2015 dbSNP
rs1399738189 2024 dbSNP
rs897716113 2024 dbSNP
rs896601648 2030 dbSNP
rs1343053593 2055 dbSNP
rs544290048 2074 dbSNP
rs1454289723 2077 dbSNP
rs993361273 2088 dbSNP
rs1176920842 2098 dbSNP
rs1439149668 2099 dbSNP
rs1243989397 2102 dbSNP
rs1279712707 2103 dbSNP
rs1049390369 2111 dbSNP
rs535672745 2112 dbSNP
rs1255850732 2115 dbSNP
rs1206068290 2126 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions C8166 , TZM-bl
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM1462572. RNA binding protein: AGO2. Condition:C8166 NL4-3 PAR-CLIP data was present in GSM1462573. RNA binding protein: AGO2. Condition:TZM-bl BaL ...

- Whisnant AW; Bogerd HP; Flores O; Ho P; et al., 2013, mBio.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' ugcaaAUUUAAAG------UGGGGAAGa 5'
               ||| ||||       ||||||| 
Target 5' --aaaUAACUUUCUUCUCUUCCCCUUCc 3'
1 - 26
Article - Whisnant AW; Bogerd HP; Flores O; Ho P; et al.
- mBio, 2013
UNLABELLED: The question of how HIV-1 interfaces with cellular microRNA (miRNA) biogenesis and effector mechanisms has been highly controversial. Here, we first used deep sequencing of small RNAs present in two different infected cell lines (TZM-bl and C8166) and two types of primary human cells (CD4(+) peripheral blood mononuclear cells [PBMCs] and macrophages) to unequivocally demonstrate that HIV-1 does not encode any viral miRNAs. Perhaps surprisingly, we also observed that infection of T cells by HIV-1 has only a modest effect on the expression of cellular miRNAs at early times after infection. Comprehensive analysis of miRNA binding to the HIV-1 genome using the photoactivatable ribonucleoside-induced cross-linking and immunoprecipitation (PAR-CLIP) technique revealed several binding sites for cellular miRNAs, a subset of which were shown to be capable of mediating miRNA-mediated repression of gene expression. However, the main finding from this analysis is that HIV-1 transcripts are largely refractory to miRNA binding, most probably due to extensive viral RNA secondary structure. Together, these data demonstrate that HIV-1 neither encodes viral miRNAs nor strongly influences cellular miRNA expression, at least early after infection, and imply that HIV-1 transcripts have evolved to avoid inhibition by preexisting cellular miRNAs by adopting extensive RNA secondary structures that occlude most potential miRNA binding sites. IMPORTANCE: MicroRNAs (miRNAs) are a ubiquitous class of small regulatory RNAs that serve as posttranscriptional regulators of gene expression. Previous work has suggested that HIV-1 might subvert the function of the cellular miRNA machinery by expressing viral miRNAs or by dramatically altering the level of cellular miRNA expression. Using very sensitive approaches, we now demonstrate that neither of these ideas is in fact correct. Moreover, HIV-1 transcripts appear to largely avoid regulation by cellular miRNAs by adopting an extensive RNA secondary structure that occludes the ability of cellular miRNAs to interact with viral mRNAs. Together, these data suggest that HIV-1, rather than seeking to control miRNA function in infected cells, has instead evolved a mechanism to become largely invisible to cellular miRNA effector mechanisms.
LinkOut: [PMID: 23592263]
CLIP-seq Support 1 for dataset GSM4903833
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / CTL_TD_21_a
Location of target site NM_014372 | 3UTR | UAACUUUCUUCUCUUCCCCUUCCCCACCCGAAUUCUUUUCUGAAGUUGCUGGCAUUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1462572
Method / RBP PAR-CLIP / AGO2
Cell line / Condition C8166 / C8166 NL4-3
Location of target site ENST00000242719.3 | 3UTR | AAAUAACUUUCUUCUCUUCCCCUUCCCCACCCG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23592263 / GSE59944
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM1462573
Method / RBP PAR-CLIP / AGO2
Cell line / Condition TZM-bl / TZM-bl BaL
Location of target site ENST00000242719.3 | 3UTR | AAAUAACUUUCUUCUCUUCCCCUUCCCCACCCG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23592263 / GSE59944
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
Click to see details
Click to see details
133 hsa-miR-3179 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT102087 GIGYF1 GRB10 interacting GYF protein 1 2 4
MIRT110061 OGT O-linked N-acetylglucosamine (GlcNAc) transferase 2 6
MIRT112198 BTG2 BTG anti-proliferation factor 2 2 2
MIRT117668 SCAMP4 secretory carrier membrane protein 4 2 2
MIRT146657 MINK1 misshapen like kinase 1 2 2
MIRT175505 ZBTB33 zinc finger and BTB domain containing 33 2 4
MIRT180535 TXNIP thioredoxin interacting protein 2 2
MIRT190624 BCL2L2-PABPN1 BCL2L2-PABPN1 readthrough 2 2
MIRT190650 PABPN1 poly(A) binding protein nuclear 1 2 2
MIRT366902 NONO non-POU domain containing octamer binding 2 2
MIRT443554 ZFP3 ZFP3 zinc finger protein 2 2
MIRT445953 MLLT11 MLLT11, transcription factor 7 cofactor 2 2
MIRT446042 HMCN1 hemicentin 1 2 2
MIRT447968 MSH6 mutS homolog 6 2 2
MIRT448634 ONECUT1 one cut homeobox 1 2 2
MIRT449316 MRO maestro 2 2
MIRT451380 C19orf43 telomerase RNA component interacting RNase 2 2
MIRT451547 CIAPIN1 cytokine induced apoptosis inhibitor 1 2 2
MIRT451807 CDCA3 cell division cycle associated 3 2 4
MIRT451916 ILK integrin linked kinase 2 2
MIRT451938 TMPRSS5 transmembrane protease, serine 5 2 2
MIRT452189 KIAA1456 KIAA1456 2 2
MIRT452498 HMGXB3 HMG-box containing 3 2 2
MIRT452548 ZNF467 zinc finger protein 467 2 2
MIRT453844 SDK1 sidekick cell adhesion molecule 1 2 2
MIRT454515 ZFYVE27 zinc finger FYVE-type containing 27 2 2
MIRT455363 KDM5C lysine demethylase 5C 2 2
MIRT455455 EPB41L4B erythrocyte membrane protein band 4.1 like 4B 2 2
MIRT455628 PABPC1L2B poly(A) binding protein cytoplasmic 1 like 2B 2 10
MIRT455639 PABPC1L2A poly(A) binding protein cytoplasmic 1 like 2A 2 10
MIRT455690 GLO1 glyoxalase I 2 2
MIRT456300 ASH1L ASH1 like histone lysine methyltransferase 2 2
MIRT456784 MTHFSD methenyltetrahydrofolate synthetase domain containing 2 2
MIRT456819 PIGP phosphatidylinositol glycan anchor biosynthesis class P 2 2
MIRT457566 ZNF34 zinc finger protein 34 2 2
MIRT457604 IDS iduronate 2-sulfatase 2 2
MIRT458236 NXPH3 neurexophilin 3 2 2
MIRT458313 TNFAIP8L3 TNF alpha induced protein 8 like 3 2 2
MIRT458350 NOC2L NOC2 like nucleolar associated transcriptional repressor 2 2
MIRT458670 GPR35 G protein-coupled receptor 35 2 2
MIRT459675 VPS37C VPS37C, ESCRT-I subunit 2 2
MIRT461126 RAB36 RAB36, member RAS oncogene family 2 2
MIRT461918 NECAB3 N-terminal EF-hand calcium binding protein 3 2 2
MIRT462301 PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H 2 2
MIRT463520 ZBTB7B zinc finger and BTB domain containing 7B 2 2
MIRT464378 URM1 ubiquitin related modifier 1 2 2
MIRT464614 UBE4B ubiquitination factor E4B 2 2
MIRT464711 UBE2V1 ubiquitin conjugating enzyme E2 V1 2 2
MIRT465520 PRICKLE4 prickle planar cell polarity protein 4 2 2
MIRT465974 TMEM189-UBE2V1 TMEM189-UBE2V1 readthrough 2 2
MIRT466058 TMEM189 transmembrane protein 189 2 2
MIRT466548 TBL1XR1 transducin beta like 1 X-linked receptor 1 2 2
MIRT466647 TAGLN2 transgelin 2 2 2
MIRT467357 SP2 Sp2 transcription factor 2 2
MIRT468744 SDC2 syndecan 2 2 2
MIRT470244 PRRC2A proline rich coiled-coil 2A 2 2
MIRT471426 PDIA6 protein disulfide isomerase family A member 6 2 2
MIRT471732 OTUB1 OTU deubiquitinase, ubiquitin aldehyde binding 1 2 2
MIRT472190 NHP2L1 small nuclear ribonucleoprotein 13 2 2
MIRT472450 NAV2 neuron navigator 2 2 6
MIRT474563 KLHDC3 kelch domain containing 3 2 2
MIRT474936 KCTD15 potassium channel tetramerization domain containing 15 2 2
MIRT475165 IP6K1 inositol hexakisphosphate kinase 1 2 2
MIRT475399 ICMT isoprenylcysteine carboxyl methyltransferase 2 4
MIRT475426 ICK intestinal cell kinase 2 2
MIRT477090 FAM168A family with sequence similarity 168 member A 2 2
MIRT478458 DAB2 DAB2, clathrin adaptor protein 2 2
MIRT478953 COX15 COX15, cytochrome c oxidase assembly homolog 2 2
MIRT480096 CALR calreticulin 2 2
MIRT481924 ANKRD33B ankyrin repeat domain 33B 2 2
MIRT483217 APOA1 apolipoprotein A1 2 6
MIRT483882 TGIF1 TGFB induced factor homeobox 1 2 2
MIRT483923 SPSB1 splA/ryanodine receptor domain and SOCS box containing 1 2 2
MIRT483942 LENG8 leukocyte receptor cluster member 8 2 4
MIRT484209 SUMO1 small ubiquitin-like modifier 1 2 2
MIRT484512 SYT7 synaptotagmin 7 2 2
MIRT484709 RNF11 ring finger protein 11 2 2
MIRT485356 MYO1C myosin IC 2 4
MIRT485615 FOSL1 FOS like 1, AP-1 transcription factor subunit 2 4
MIRT486584 ZNF619 zinc finger protein 619 2 2
MIRT487013 C2orf82 chromosome 2 open reading frame 82 2 2
MIRT487621 C20orf96 chromosome 20 open reading frame 96 2 2
MIRT487801 GPR20 G protein-coupled receptor 20 2 4
MIRT488134 GPR107 G protein-coupled receptor 107 2 2
MIRT488773 FXYD1 FXYD domain containing ion transport regulator 1 2 2
MIRT488854 UBTF upstream binding transcription factor, RNA polymerase I 2 2
MIRT489783 GRINA glutamate ionotropic receptor NMDA type subunit associated protein 1 2 2
MIRT490102 FN3K fructosamine 3 kinase 2 2
MIRT490389 LHFPL3 LHFPL tetraspan subfamily member 3 2 2
MIRT490434 MYL9 myosin light chain 9 2 2
MIRT490451 GLUD1 glutamate dehydrogenase 1 2 2
MIRT490880 OSBP oxysterol binding protein 2 2
MIRT491037 ALPK3 alpha kinase 3 2 2
MIRT491250 HCN2 hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 2 2
MIRT491748 SEMA3F semaphorin 3F 2 2
MIRT492235 SLC48A1 solute carrier family 48 member 1 2 2
MIRT492490 RAPGEF1 Rap guanine nucleotide exchange factor 1 2 2
MIRT492505 RANBP10 RAN binding protein 10 2 4
MIRT492773 PDGFB platelet derived growth factor subunit B 2 2
MIRT492922 NFAT5 nuclear factor of activated T-cells 5 2 2
MIRT493459 ITFG3 family with sequence similarity 234 member A 2 2
MIRT493654 HDLBP high density lipoprotein binding protein 2 2
MIRT494011 DUSP9 dual specificity phosphatase 9 2 2
MIRT499412 PLCG2 phospholipase C gamma 2 2 4
MIRT499552 C15orf43 telomere repeat binding bouquet formation protein 2 2 2
MIRT501836 NCOA2 nuclear receptor coactivator 2 2 2
MIRT501950 MAT2A methionine adenosyltransferase 2A 2 10
MIRT504066 KCTD12 potassium channel tetramerization domain containing 12 2 4
MIRT504509 PPP1R9B protein phosphatase 1 regulatory subunit 9B 2 2
MIRT508466 HOXB6 homeobox B6 2 4
MIRT512373 CPM carboxypeptidase M 2 2
MIRT513578 EVX1 even-skipped homeobox 1 2 2
MIRT517763 ZNF366 zinc finger protein 366 2 4
MIRT519773 ZNF354B zinc finger protein 354B 2 8
MIRT523568 GGCX gamma-glutamyl carboxylase 2 4
MIRT532802 CLDN11 claudin 11 2 2
MIRT544299 TSPYL1 TSPY like 1 2 2
MIRT544862 MYH2 myosin heavy chain 2 2 4
MIRT556731 KLHL15 kelch like family member 15 2 4
MIRT564347 AKR1B10 aldo-keto reductase family 1 member B10 2 2
MIRT568924 SMCR8 Smith-Magenis syndrome chromosome region, candidate 8 2 2
MIRT569012 CXorf36 chromosome X open reading frame 36 2 2
MIRT569256 FAM129B family with sequence similarity 129 member B 2 2
MIRT569591 PRELP proline and arginine rich end leucine rich repeat protein 2 2
MIRT569779 SAMD14 sterile alpha motif domain containing 14 2 2
MIRT570034 FAM228A family with sequence similarity 228 member A 2 2
MIRT573803 FRMPD4 FERM and PDZ domain containing 4 2 2
MIRT574190 ZNF264 zinc finger protein 264 2 2
MIRT576153 Hmox1 heme oxygenase 1 2 2
MIRT611311 CA8 carbonic anhydrase 8 2 4
MIRT673429 APAF1 apoptotic peptidase activating factor 1 2 2
MIRT674976 SH3BP2 SH3 domain binding protein 2 2 2
MIRT692712 MEAF6 MYST/Esa1 associated factor 6 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-3179 Gemcitabine 60750 NSC613327 approved resistant High Pancreatic Cancer cell line (AsPC-1)
hsa-miR-3179 Gefitinib 123631 NSC715055 approved sensitive cell line (HCC827)
hsa-miR-3179 Gefitinib 123631 NSC715055 approved sensitive cell line (PC9)
hsa-miR-3179 Osimertinib 71496458 NSC779217 approved sensitive cell line (HCC827)
hsa-miR-3179 Osimertinib 71496458 NSC779217 approved sensitive cell line (PC9)
hsa-miR-3179 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-3179 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)
hsa-miR-3179 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)

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