pre-miRNA Information
pre-miRNA hsa-mir-4433a   
Genomic Coordinates chr2: 64340759 - 64340839
Description Homo sapiens miR-4433a stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4433a-3p
Sequence 51| ACAGGAGUGGGGGUGGGACAU |71
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs751480515 9 dbSNP
rs557760377 15 dbSNP
rs1479580865 20 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol FAM3C   
Synonyms GS3786, ILEI
Description family with sequence similarity 3 member C
Transcript NM_001040020   
Other Transcripts NM_014888   
Expression
Putative miRNA Targets on FAM3C
3'UTR of FAM3C
(miRNA target sites are highlighted)
>FAM3C|NM_001040020|3'UTR
   1 TGGAAATGTGGAGAGAATTGAAGAAAGCGCACTTTCACTCTTAATGGGAGAGCTATAAATGGCAGAGCTATGTGTAAATA
  81 TTTTAAGAGCATGCAGCCATCTTGGTGTGTGCATGAGTATTGTCTCTTTTGATATCAGGATTATTTATTGCTAACGTAAA
 161 TAGATAGCATTGTAAATAATCATCACAATGATCAAATCACTGAACCATGTCTCCGCACATTTCCCTAAAAGTACAATGTT
 241 TAGACTGCTATGGTAATACATATTTTAAATTCTAAAAGCATACACAATGTGTAACTGAATGGTTTGTGAAAAATATATTG
 321 ATATATATACTAGTTGCTATGAAAATATCATGGAATAATAGGGATTTTAGGGTGGATACTTTATTTTCTTTTATGTTTCT
 401 ATATGTTGCGTTGTGATGACATTATCTTTTAAATTAAAAAGAGATTTGGCTAGTTGTGTGTGTAATGTTACTTTACAGTC
 481 CGACTCTCCTGATGTACCTCTTTTCATGATCTTTTTCTTTCCTTCCCAAGAAACTGAGGAATGTTTAATATGAAAACATA
 561 CATCGGATATGTGAAAAGCACAACAAAATTCTTAATGTACACAGTAAAAAAGTAAATATATAAATGTAGATGGCATTTAG
 641 GACCACAGCTTGCTGGATTTGTGTTAGCTATGGGAATAACTTGATTTTGTATAAGCTATTTAGAGTGAGGCTGGAGGTGG
 721 CAGCTTCACAGAACTGGAGAACCAGGCCAAGTCCCCTCCCCAACCTAATTAGGTCATTCAGGACAGCTAAGTCAGTATAT
 801 TTAGAGCAATACTAGCATACGTTTTTCTTAATTGTTATCAGCATTGACCAAGTGGTTTGGAAGGAGGCATGCTTTAATAT
 881 CACAATAATTTTGATTTGTAAACCAAGAAATTAATCCTGTGTTTATCTAACTTCATAATAGCAATTATTGCCCGAAGCTA
 961 TAGTGGCATATTTACAAAAGTTCTTATTACTGGGCGGACTGATAACATTTAAAAAATAATTGTGTTTGACCCCAAATGAC
1041 TTTATACCCAATTCTACATAAAAATATAGAAGATCTATCTTTTTTTGTTACCTTCAGATGTTCACTAAATAACTCAGTTT
1121 TTAAGCAGAAGTTTTCAGGGCATTAAATATATGTTGTGTATGAAGTATCTCAAACTGGAACATAAATTTAGTGATCAAAC
1201 TGCCATTCACAGTGTAAGGCAGCACTTAAATTTCGAACCTAAAGTTTAGATGCATTGTATAAAAAAACCTAAAAGCAGTA
1281 TCTGTTATTTAGCTGTAAACCAAGTTGGAAGCTATTCGGATAATTTCTTAAATATTGATGAACTTTGGAGTACTGTTTCT
1361 TCCTTCAAACTGAATGTAATTAATTCATGAATAAATGCACCTTATATGTTTAAACAATCTTTGTATACTTTTGGGATTTT
1441 TGGTGCTTATATGCTAAATCACATTCAGCATGTGTATTTTGACATTTAAAATACTTCCCTCAATTCTGTAAATTAAAAGA
1521 ATAGTTATTTTACAGTTCCAGGGATTGTGAAATAAATGTTGCAGTTTTTTAAAATAATGAAAATAAATACTCTTGGTTTT
1601 GCTTTGTGAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' uacaGGGUGGGGGUGAGGAca 5'
              | |||:::|||||:|  
Target 5' aaagCGCACTTTCACTCTTaa 3'
24 - 44 129.00 -18.60
2
miRNA  3' uaCAGGGUGGGGGUGAGGACa 5'
            |||| ||:    |||||| 
Target 5' caGTCCGACT----CTCCTGa 3'
476 - 492 128.00 -17.10
3
miRNA  3' uacaggguggggGUGAGGACa 5'
                      :||||:|| 
Target 5' aatgaaaataaaTACTCTTGg 3'
1576 - 1596 125.00 -9.30
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN31514622 28 COSMIC
COSN30166683 29 COSMIC
COSN31510895 60 COSMIC
COSN30148626 101 COSMIC
COSN31506293 105 COSMIC
COSN31559171 145 COSMIC
COSN31582234 152 COSMIC
COSN31591310 184 COSMIC
COSN2197267 323 COSMIC
COSN32055833 395 COSMIC
COSN31545078 420 COSMIC
COSN30161320 718 COSMIC
COSN17965718 831 COSMIC
COSN7956164 1096 COSMIC
COSN2514264 1115 COSMIC
COSN26410629 1167 COSMIC
COSN31488234 1203 COSMIC
COSN31962845 1317 COSMIC
COSN31530350 1323 COSMIC
COSN31582884 1340 COSMIC
COSN31521667 1477 COSMIC
COSN31517065 1511 COSMIC
COSN21493834 1561 COSMIC
rs15427 395 GWAS
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs780077708 3 dbSNP
rs200476093 6 dbSNP
rs367676796 7 dbSNP
rs1362578947 10 dbSNP
rs767435779 18 dbSNP
rs949121932 22 dbSNP
rs1420119663 24 dbSNP
rs1257773854 26 dbSNP
rs761495081 27 dbSNP
rs751327784 28 dbSNP
rs763685259 29 dbSNP
rs762573092 32 dbSNP
rs1224837930 36 dbSNP
rs771429808 38 dbSNP
rs1258961778 42 dbSNP
rs1238045808 44 dbSNP
rs1451309475 48 dbSNP
rs761098288 50 dbSNP
rs1489308927 51 dbSNP
rs990891636 53 dbSNP
rs1023566 60 dbSNP
rs959106887 70 dbSNP
rs1298213028 72 dbSNP
rs1422599792 73 dbSNP
rs1035002221 89 dbSNP
rs1164829432 91 dbSNP
rs377269503 93 dbSNP
rs979060703 105 dbSNP
rs1292051614 115 dbSNP
rs968835457 117 dbSNP
rs1398652872 134 dbSNP
rs1278091676 140 dbSNP
rs1356715716 144 dbSNP
rs1247426868 147 dbSNP
rs1020423999 152 dbSNP
rs1355825254 153 dbSNP
rs1010235945 155 dbSNP
rs1288192074 160 dbSNP
rs1403015926 163 dbSNP
rs1297759041 165 dbSNP
rs1216031868 167 dbSNP
rs1262356210 168 dbSNP
rs1424383041 169 dbSNP
rs1189349986 175 dbSNP
rs1366515671 181 dbSNP
rs553060478 182 dbSNP
rs1158909621 186 dbSNP
rs1030449730 199 dbSNP
rs996567668 205 dbSNP
rs536763949 214 dbSNP
rs1403531260 215 dbSNP
rs1172527518 231 dbSNP
rs1465985846 235 dbSNP
rs1340272414 236 dbSNP
rs1417078874 241 dbSNP
rs1311696045 245 dbSNP
rs1185793158 246 dbSNP
rs574004929 251 dbSNP
rs1424985208 259 dbSNP
rs1037996416 260 dbSNP
rs1483505887 263 dbSNP
rs1202133205 279 dbSNP
rs1253775372 283 dbSNP
rs1256279396 284 dbSNP
rs557582464 289 dbSNP
rs1479439314 294 dbSNP
rs537623289 312 dbSNP
rs1199657521 314 dbSNP
rs1377710844 316 dbSNP
rs886238272 325 dbSNP
rs1171368075 327 dbSNP
rs1334675617 330 dbSNP
rs1447949105 336 dbSNP
rs549677483 337 dbSNP
rs3295 339 dbSNP
rs1446324985 344 dbSNP
rs1302015666 351 dbSNP
rs1314056794 359 dbSNP
rs1341100710 363 dbSNP
rs190325175 368 dbSNP
rs1389797056 371 dbSNP
rs1046709 388 dbSNP
rs1375761054 392 dbSNP
rs15426 393 dbSNP
rs535298560 394 dbSNP
rs15427 395 dbSNP
rs949241094 399 dbSNP
rs551577326 401 dbSNP
rs1393476723 402 dbSNP
rs1162625645 409 dbSNP
rs548653685 410 dbSNP
rs990616103 415 dbSNP
rs1196190272 416 dbSNP
rs1377544092 426 dbSNP
rs937655607 426 dbSNP
rs529030048 448 dbSNP
rs1173183730 453 dbSNP
rs1398590998 458 dbSNP
rs1465265520 460 dbSNP
rs1294311750 464 dbSNP
rs1369034100 481 dbSNP
rs924991167 482 dbSNP
rs1416857685 490 dbSNP
rs1300758166 491 dbSNP
rs1344724950 509 dbSNP
rs1239602944 525 dbSNP
rs979395937 526 dbSNP
rs1178879818 529 dbSNP
rs1468264289 536 dbSNP
rs1265640004 538 dbSNP
rs1251555010 544 dbSNP
rs1212105048 560 dbSNP
rs1266055808 564 dbSNP
rs1478399787 565 dbSNP
rs968951610 567 dbSNP
rs1422163647 569 dbSNP
rs1477688820 571 dbSNP
rs766243816 573 dbSNP
rs1236973976 576 dbSNP
rs11560 578 dbSNP
rs1020531990 599 dbSNP
rs988929890 605 dbSNP
rs1361274238 606 dbSNP
rs1310192814 609 dbSNP
rs1400491519 611 dbSNP
rs1279982651 616 dbSNP
rs1338399836 627 dbSNP
rs569640372 631 dbSNP
rs1369907173 661 dbSNP
rs1218941251 662 dbSNP
rs1323616679 666 dbSNP
rs1388334942 670 dbSNP
rs1261640264 689 dbSNP
rs1391354573 691 dbSNP
rs954903469 694 dbSNP
rs1450926009 696 dbSNP
rs1422235259 698 dbSNP
rs1381629082 705 dbSNP
rs1158833966 706 dbSNP
rs1421566709 714 dbSNP
rs1453445972 716 dbSNP
rs879180954 718 dbSNP
rs1201422236 720 dbSNP
rs1030810969 722 dbSNP
rs1303150423 723 dbSNP
rs1381098233 734 dbSNP
rs1226440981 742 dbSNP
rs996293801 761 dbSNP
rs965041359 767 dbSNP
rs1203915642 775 dbSNP
rs1241684011 787 dbSNP
rs1336256335 791 dbSNP
rs1489275234 796 dbSNP
rs140563003 797 dbSNP
rs1239539820 798 dbSNP
rs1482462436 799 dbSNP
rs1233719890 800 dbSNP
rs1181686075 804 dbSNP
rs532941147 809 dbSNP
rs563911759 812 dbSNP
rs1156853760 813 dbSNP
rs540942710 817 dbSNP
rs1399988220 820 dbSNP
rs1345951633 821 dbSNP
rs1360876395 831 dbSNP
rs1445531148 834 dbSNP
rs1335921590 835 dbSNP
rs1803389 836 dbSNP
rs1235120322 838 dbSNP
rs1306396986 853 dbSNP
rs1349372065 862 dbSNP
rs559702514 863 dbSNP
rs542654760 864 dbSNP
rs1281320619 868 dbSNP
rs1047711652 871 dbSNP
rs1398103671 884 dbSNP
rs542968925 885 dbSNP
rs1361839926 886 dbSNP
rs1013411823 891 dbSNP
rs1433606094 907 dbSNP
rs1373235910 929 dbSNP
rs574121233 930 dbSNP
rs896345389 931 dbSNP
rs1055261735 939 dbSNP
rs1478530215 951 dbSNP
rs1400793236 953 dbSNP
rs1466828352 954 dbSNP
rs1328834774 955 dbSNP
rs1372875087 957 dbSNP
rs1191618441 959 dbSNP
rs1157380634 960 dbSNP
rs1479074310 961 dbSNP
rs937790845 962 dbSNP
rs1366023620 965 dbSNP
rs924908189 975 dbSNP
rs557151399 977 dbSNP
rs1259049520 978 dbSNP
rs2109807 984 dbSNP
rs1197202464 985 dbSNP
rs1272549667 987 dbSNP
rs1210242757 992 dbSNP
rs945076684 993 dbSNP
rs34073083 995 dbSNP
rs578225474 996 dbSNP
rs117635009 998 dbSNP
rs35717279 1006 dbSNP
rs1165997314 1010 dbSNP
rs1395315379 1011 dbSNP
rs1405599160 1018 dbSNP
rs1269399097 1022 dbSNP
rs1347629953 1024 dbSNP
rs1404834405 1028 dbSNP
rs1031763168 1030 dbSNP
rs1279351286 1044 dbSNP
rs955020348 1047 dbSNP
rs1269988449 1058 dbSNP
rs1357253730 1059 dbSNP
rs35021323 1069 dbSNP
rs563943688 1077 dbSNP
rs1278841902 1087 dbSNP
rs566240478 1091 dbSNP
rs1490198817 1096 dbSNP
rs975198295 1109 dbSNP
rs1489267819 1117 dbSNP
rs1338210119 1150 dbSNP
rs1335712381 1151 dbSNP
rs1372302343 1165 dbSNP
rs1472892456 1169 dbSNP
rs1164664381 1195 dbSNP
rs555381139 1211 dbSNP
rs544155636 1234 dbSNP
rs578205496 1235 dbSNP
rs1006219294 1241 dbSNP
rs35676736 1243 dbSNP
rs1467572655 1247 dbSNP
rs1362937438 1252 dbSNP
rs1424948586 1253 dbSNP
rs1398714421 1268 dbSNP
rs535087721 1268 dbSNP
rs1341477755 1270 dbSNP
rs950724242 1270 dbSNP
rs1026218111 1276 dbSNP
rs1013529576 1278 dbSNP
rs1240956671 1281 dbSNP
rs1288258262 1282 dbSNP
rs1355693012 1286 dbSNP
rs1187876724 1295 dbSNP
rs140861518 1317 dbSNP
rs1239318354 1318 dbSNP
rs1054975952 1324 dbSNP
rs1188446740 1340 dbSNP
rs2159720 1353 dbSNP
rs903592474 1361 dbSNP
rs1043482286 1363 dbSNP
rs1196526349 1364 dbSNP
rs1158465869 1373 dbSNP
rs572058348 1387 dbSNP
rs1258572107 1399 dbSNP
rs1280179261 1404 dbSNP
rs1353599931 1405 dbSNP
rs1447811533 1406 dbSNP
rs1311492258 1417 dbSNP
rs1285474790 1423 dbSNP
rs1238467045 1428 dbSNP
rs532830308 1432 dbSNP
rs1334797737 1441 dbSNP
rs2536183 1447 dbSNP
rs570325273 1468 dbSNP
rs1460566441 1471 dbSNP
rs1178900801 1484 dbSNP
rs1239664648 1486 dbSNP
rs1449381461 1489 dbSNP
rs933595530 1492 dbSNP
rs547146866 1493 dbSNP
rs1401485606 1497 dbSNP
rs923393683 1509 dbSNP
rs1468032442 1514 dbSNP
rs974934682 1521 dbSNP
rs1405836957 1527 dbSNP
rs1466299651 1529 dbSNP
rs1333578586 1548 dbSNP
rs1371509731 1558 dbSNP
rs527632936 1565 dbSNP
rs965281851 1570 dbSNP
rs561827979 1581 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545212. RNA binding protein: AGO1. Condition:Control PAR-CLIP data was present in GSM545213. RNA binding protein: AGO2. Condition:Control PAR-CLIP data was present in GSM545214. RNA binding protein: AGO3. Condition:Control PAR-CLIP data was present in GSM545215. RNA binding protein: AGO4. Condition:Control ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 10447.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM714645. RNA binding protein: AGO2. Condition:completeT1 ...

- Kishore S; Jaskiewicz L; Burger L; Hausser et al., 2011, Nature methods.

Article - Kishore S; Jaskiewicz L; Burger L; Hausser et al.
- Nature methods, 2011
Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions.
LinkOut: [PMID: 21572407]
Experimental Support 3 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions hESCs (WA-09)
Disease 10447.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in SRR359787. RNA binding protein: AGO2. Condition:4-thiouridine ...

- Lipchina I; Elkabetz Y; Hafner M; Sheridan et al., 2011, Genes & development.

Article - Lipchina I; Elkabetz Y; Hafner M; Sheridan et al.
- Genes & development, 2011
MicroRNAs are important regulators in many cellular processes, including stem cell self-renewal. Recent studies demonstrated their function as pluripotency factors with the capacity for somatic cell reprogramming. However, their role in human embryonic stem (ES) cells (hESCs) remains poorly understood, partially due to the lack of genome-wide strategies to identify their targets. Here, we performed comprehensive microRNA profiling in hESCs and in purified neural and mesenchymal derivatives. Using a combination of AGO cross-linking and microRNA perturbation experiments, together with computational prediction, we identified the targets of the miR-302/367 cluster, the most abundant microRNAs in hESCs. Functional studies identified novel roles of miR-302/367 in maintaining pluripotency and regulating hESC differentiation. We show that in addition to its role in TGF-beta signaling, miR-302/367 promotes bone morphogenetic protein (BMP) signaling by targeting BMP inhibitors TOB2, DAZAP2, and SLAIN1. This study broadens our understanding of microRNA function in hESCs and is a valuable resource for future studies in this area.
LinkOut: [PMID: 22012620]
Experimental Support 4 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions C8166
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM1462572. RNA binding protein: AGO2. Condition:C8166 NL4-3 ...

- Whisnant AW; Bogerd HP; Flores O; Ho P; et al., 2013, mBio.

Article - Whisnant AW; Bogerd HP; Flores O; Ho P; et al.
- mBio, 2013
UNLABELLED: The question of how HIV-1 interfaces with cellular microRNA (miRNA) biogenesis and effector mechanisms has been highly controversial. Here, we first used deep sequencing of small RNAs present in two different infected cell lines (TZM-bl and C8166) and two types of primary human cells (CD4(+) peripheral blood mononuclear cells [PBMCs] and macrophages) to unequivocally demonstrate that HIV-1 does not encode any viral miRNAs. Perhaps surprisingly, we also observed that infection of T cells by HIV-1 has only a modest effect on the expression of cellular miRNAs at early times after infection. Comprehensive analysis of miRNA binding to the HIV-1 genome using the photoactivatable ribonucleoside-induced cross-linking and immunoprecipitation (PAR-CLIP) technique revealed several binding sites for cellular miRNAs, a subset of which were shown to be capable of mediating miRNA-mediated repression of gene expression. However, the main finding from this analysis is that HIV-1 transcripts are largely refractory to miRNA binding, most probably due to extensive viral RNA secondary structure. Together, these data demonstrate that HIV-1 neither encodes viral miRNAs nor strongly influences cellular miRNA expression, at least early after infection, and imply that HIV-1 transcripts have evolved to avoid inhibition by preexisting cellular miRNAs by adopting extensive RNA secondary structures that occlude most potential miRNA binding sites. IMPORTANCE: MicroRNAs (miRNAs) are a ubiquitous class of small regulatory RNAs that serve as posttranscriptional regulators of gene expression. Previous work has suggested that HIV-1 might subvert the function of the cellular miRNA machinery by expressing viral miRNAs or by dramatically altering the level of cellular miRNA expression. Using very sensitive approaches, we now demonstrate that neither of these ideas is in fact correct. Moreover, HIV-1 transcripts appear to largely avoid regulation by cellular miRNAs by adopting an extensive RNA secondary structure that occludes the ability of cellular miRNAs to interact with viral mRNAs. Together, these data suggest that HIV-1, rather than seeking to control miRNA function in infected cells, has instead evolved a mechanism to become largely invisible to cellular miRNA effector mechanisms.
LinkOut: [PMID: 23592263]
CLIP-seq Support 1 for dataset GSM545212
Method / RBP PAR-CLIP / AGO1
Cell line / Condition HEK293 / Control
Location of target site ENST00000359943.3 | 3UTR | ACUUUACAGUCCGACUCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM545213
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / Control
Location of target site ENST00000359943.3 | 3UTR | ACUUUACAGUCCGACUCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM545214
Method / RBP PAR-CLIP / AGO3
Cell line / Condition HEK293 / Control
Location of target site ENST00000359943.3 | 3UTR | ACUUUACAGUCCGACUCUCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM545215
Method / RBP PAR-CLIP / AGO4
Cell line / Condition HEK293 / Control
Location of target site ENST00000359943.3 | 3UTR | ACUUUACAGUCCGACUCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM714645
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / completeT1, repB
Location of target site ENST00000359943.3 | 3UTR | ACUUUACAGUCCGACUCUCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21572407 / GSE28865
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset SRR359787
Method / RBP PAR-CLIP / AGO2
Cell line / Condition hESCs (WA-09) / 4-thiouridine, RNase T1
Location of target site ENST00000359943.3 | 3UTR | ACUUUACAGUCCGACUCUCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 22012620 / SRX103431
CLIP-seq Viewer Link
CLIP-seq Support 7 for dataset GSM1462572
Method / RBP PAR-CLIP / AGO2
Cell line / Condition C8166 / C8166 NL4-3
Location of target site ENST00000359943.3 | 3UTR | CUUUACAGUCCGACUCUCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23592263 / GSE59944
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
256 hsa-miR-4433a-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT088097 SEPT2 septin 2 2 2
MIRT143134 MGRN1 mahogunin ring finger 1 2 2
MIRT153912 NCOA3 nuclear receptor coactivator 3 2 2
MIRT154894 GNAS GNAS complex locus 2 4
MIRT200996 ZNF805 zinc finger protein 805 2 2
MIRT215729 C5ORF51 chromosome 5 open reading frame 51 2 10
MIRT235593 POFUT1 protein O-fucosyltransferase 1 2 2
MIRT263250 SGPL1 sphingosine-1-phosphate lyase 1 2 2
MIRT317951 CDC5L cell division cycle 5 like 2 4
MIRT325572 HIATL1 major facilitator superfamily domain containing 14B 2 4
MIRT354739 LSM3 LSM3 homolog, U6 small nuclear RNA and mRNA degradation associated 2 2
MIRT444552 UBE2D3 ubiquitin conjugating enzyme E2 D3 2 4
MIRT446978 SUSD5 sushi domain containing 5 2 2
MIRT451036 ZNF610 zinc finger protein 610 2 2
MIRT451596 TRPM7 transient receptor potential cation channel subfamily M member 7 2 2
MIRT452025 NLRP6 NLR family pyrin domain containing 6 2 2
MIRT452594 CA6 carbonic anhydrase 6 2 2
MIRT452768 TCEA3 transcription elongation factor A3 2 4
MIRT452959 ZNF844 zinc finger protein 844 2 2
MIRT453191 ACSF2 acyl-CoA synthetase family member 2 2 2
MIRT453382 RHD Rh blood group D antigen 2 2
MIRT453685 CEBPD CCAAT/enhancer binding protein delta 2 2
MIRT455272 DDX39B DExD-box helicase 39B 2 8
MIRT455346 BAMBI BMP and activin membrane bound inhibitor 2 2
MIRT456494 SERAC1 serine active site containing 1 2 2
MIRT456585 NID1 nidogen 1 2 2
MIRT456888 DDA1 DET1 and DDB1 associated 1 2 2
MIRT457123 APOLD1 apolipoprotein L domain containing 1 2 2
MIRT457852 ZNF324B zinc finger protein 324B 2 2
MIRT457987 APAF1 apoptotic peptidase activating factor 1 2 2
MIRT459278 APOBEC3F apolipoprotein B mRNA editing enzyme catalytic subunit 3F 2 2
MIRT459625 SLC25A33 solute carrier family 25 member 33 2 2
MIRT459715 SGK494 uncharacterized serine/threonine-protein kinase SgK494 2 2
MIRT460057 RPL22L1 ribosomal protein L22 like 1 2 2
MIRT460182 UNK unkempt family zinc finger 2 6
MIRT460288 PDE11A phosphodiesterase 11A 2 2
MIRT460841 EGF epidermal growth factor 2 4
MIRT460860 TBC1D19 TBC1 domain family member 19 2 2
MIRT461519 EMC7 ER membrane protein complex subunit 7 2 2
MIRT461729 SLC27A1 solute carrier family 27 member 1 2 4
MIRT461821 SNAP23 synaptosome associated protein 23 2 2
MIRT462066 CCDC77 coiled-coil domain containing 77 2 4
MIRT462084 MSANTD2 Myb/SANT DNA binding domain containing 2 2 2
MIRT462508 MTFMT mitochondrial methionyl-tRNA formyltransferase 2 10
MIRT464239 VCP valosin containing protein 2 2
MIRT465316 TRAF5 TNF receptor associated factor 5 2 2
MIRT466549 TBL1XR1 transducin beta like 1 X-linked receptor 1 2 2
MIRT467128 SRGAP1 SLIT-ROBO Rho GTPase activating protein 1 2 8
MIRT468091 SHCBP1 SHC binding and spindle associated 1 2 2
MIRT469630 RAD21 RAD21 cohesin complex component 2 6
MIRT471097 PIK3C2B phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta 2 2
MIRT472704 MYBL1 MYB proto-oncogene like 1 2 2
MIRT472830 MTMR10 myotubularin related protein 10 2 2
MIRT472872 MTHFD2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase 2 2
MIRT472895 MTDH metadherin 2 2
MIRT473728 MAPK1 mitogen-activated protein kinase 1 2 2
MIRT473859 MAP2K4 mitogen-activated protein kinase kinase 4 2 2
MIRT474047 LONRF1 LON peptidase N-terminal domain and ring finger 1 2 2
MIRT474297 LAMC1 laminin subunit gamma 1 2 2
MIRT474658 KLF13 Kruppel like factor 13 2 2
MIRT475234 IKZF3 IKAROS family zinc finger 3 2 2
MIRT475500 HSP90B1 heat shock protein 90 beta family member 1 2 2
MIRT475742 HERPUD1 homocysteine inducible ER protein with ubiquitin like domain 1 2 4
MIRT475793 HDGF heparin binding growth factor 2 2
MIRT477254 ERGIC2 ERGIC and golgi 2 2 2
MIRT478228 DDX52 DExD-box helicase 52 2 2
MIRT478393 DCTN5 dynactin subunit 5 2 2
MIRT478754 CS citrate synthase 2 2
MIRT478827 CRKL CRK like proto-oncogene, adaptor protein 2 4
MIRT480234 C9orf41 carnosine N-methyltransferase 1 2 2
MIRT480597 BTRC beta-transducin repeat containing E3 ubiquitin protein ligase 2 2
MIRT484121 C14orf142 GON7, KEOPS complex subunit homolog 2 2
MIRT484689 PACSIN1 protein kinase C and casein kinase substrate in neurons 1 2 2
MIRT486331 C11orf54 chromosome 11 open reading frame 54 2 4
MIRT488599 FAM3C family with sequence similarity 3 member C 2 8
MIRT488833 MRRF mitochondrial ribosome recycling factor 2 2
MIRT492523 RAB15 RAB15, member RAS oncogene family 2 4
MIRT493632 HIC2 HIC ZBTB transcriptional repressor 2 2 2
MIRT500026 ABCF2 ATP binding cassette subfamily F member 2 2 8
MIRT501075 SMAD7 SMAD family member 7 2 8
MIRT503094 BTG2 BTG anti-proliferation factor 2 2 4
MIRT503336 MMAB methylmalonic aciduria (cobalamin deficiency) cblB type 2 2
MIRT505465 STMN1 stathmin 1 2 4
MIRT505726 SERTAD3 SERTA domain containing 3 2 4
MIRT509333 MS4A4A membrane spanning 4-domains A4A 2 2
MIRT509978 KCNMB1 potassium calcium-activated channel subfamily M regulatory beta subunit 1 2 4
MIRT513068 CHST6 carbohydrate sulfotransferase 6 2 2
MIRT513446 EMP1 epithelial membrane protein 1 2 6
MIRT513736 PSD3 pleckstrin and Sec7 domain containing 3 2 4
MIRT513996 CENPQ centromere protein Q 2 4
MIRT516538 MIXL1 Mix paired-like homeobox 2 2
MIRT517606 SAV1 salvador family WW domain containing protein 1 2 2
MIRT518064 CEP89 centrosomal protein 89 2 2
MIRT518119 RNMTL1 mitochondrial rRNA methyltransferase 3 2 2
MIRT518325 WDR92 WD repeat domain 92 2 2
MIRT519048 ABCB11 ATP binding cassette subfamily B member 11 2 2
MIRT520972 SPPL2A signal peptide peptidase like 2A 2 4
MIRT521359 RPL35A ribosomal protein L35a 2 2
MIRT523359 GTF3C6 general transcription factor IIIC subunit 6 2 2
MIRT523801 FAM63A MINDY lysine 48 deubiquitinase 1 2 2
MIRT524622 C7orf73 short transmembrane mitochondrial protein 1 2 2
MIRT525550 PHB2 prohibitin 2 2 4
MIRT529709 ZBTB49 zinc finger and BTB domain containing 49 2 2
MIRT531605 PLEKHA6 pleckstrin homology domain containing A6 2 2
MIRT532387 UMPS uridine monophosphate synthetase 2 2
MIRT534468 SCD stearoyl-CoA desaturase 2 4
MIRT537546 ETNK1 ethanolamine kinase 1 2 2
MIRT541619 C11orf31 selenoprotein H 2 2
MIRT548380 ENPP5 ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative) 2 4
MIRT549523 HDDC2 HD domain containing 2 2 2
MIRT549774 SOD2 superoxide dismutase 2 2 2
MIRT550578 SLC2A5 solute carrier family 2 member 5 2 2
MIRT551498 CENPN centromere protein N 2 4
MIRT552301 ITGA3 integrin subunit alpha 3 2 2
MIRT555400 PPM1L protein phosphatase, Mg2+/Mn2+ dependent 1L 2 2
MIRT556399 LUC7L LUC7 like 2 2
MIRT557047 HOXB3 homeobox B3 2 2
MIRT558072 ERO1L endoplasmic reticulum oxidoreductase 1 alpha 1 2
MIRT559659 AHCYL2 adenosylhomocysteinase like 2 2 2
MIRT561239 ZNF354B zinc finger protein 354B 2 2
MIRT566899 LRIG2 leucine rich repeats and immunoglobulin like domains 2 2 2
MIRT568786 FAM120B family with sequence similarity 120B 2 2
MIRT574014 MRPL12 mitochondrial ribosomal protein L12 2 2
MIRT574885 Dnajc6 DnaJ heat shock protein family (Hsp40) member C6 2 2
MIRT575243 Serping1 serine (or cysteine) peptidase inhibitor, clade G, member 1 2 2
MIRT576194 Vsig2 V-set and immunoglobulin domain containing 2 2 2
MIRT576309 Acbd7 acyl-Coenzyme A binding domain containing 7 2 2
MIRT576484 Lhx4 LIM homeobox protein 4 2 3
MIRT576646 Mill2 MHC I like leukocyte 2 1 1
MIRT576708 Kras Kirsten rat sarcoma viral oncogene homolog 2 2
MIRT576855 Socs6 suppressor of cytokine signaling 6 2 2
MIRT576950 Aldoa aldolase A, fructose-bisphosphate 2 2
MIRT617133 ZNF556 zinc finger protein 556 2 4
MIRT617928 ZNF783 zinc finger family member 783 2 2
MIRT618981 MRPS16 mitochondrial ribosomal protein S16 2 2
MIRT621100 SIX3 SIX homeobox 3 2 2
MIRT624537 BROX BRO1 domain and CAAX motif containing 2 2
MIRT625663 C2orf48 chromosome 2 open reading frame 48 2 2
MIRT627059 DCTN6 dynactin subunit 6 2 2
MIRT628319 CLPB ClpB homolog, mitochondrial AAA ATPase chaperonin 2 2
MIRT630859 ENTPD5 ectonucleoside triphosphate diphosphohydrolase 5 2 2
MIRT632295 TMEM65 transmembrane protein 65 2 2
MIRT634115 ZNF207 zinc finger protein 207 2 2
MIRT634736 CYP20A1 cytochrome P450 family 20 subfamily A member 1 2 2
MIRT635655 NDST3 N-deacetylase and N-sulfotransferase 3 2 2
MIRT635772 PDCL3 phosducin like 3 2 2
MIRT635911 LILRA2 leukocyte immunoglobulin like receptor A2 2 2
MIRT636365 OGFRL1 opioid growth factor receptor like 1 2 4
MIRT637251 GLRX2 glutaredoxin 2 2 2
MIRT638705 FZD4 frizzled class receptor 4 2 2
MIRT639640 PREX2 phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2 2 2
MIRT639676 PPEF2 protein phosphatase with EF-hand domain 2 2 4
MIRT642267 SMIM17 small integral membrane protein 17 2 2
MIRT642372 ZNF581 zinc finger protein 581 2 2
MIRT643545 SLC25A17 solute carrier family 25 member 17 2 2
MIRT647994 PDE12 phosphodiesterase 12 2 2
MIRT649094 NOM1 nucleolar protein with MIF4G domain 1 2 2
MIRT650993 ZNF770 zinc finger protein 770 2 2
MIRT651956 UBE2N ubiquitin conjugating enzyme E2 N 2 2
MIRT652977 SUN2 Sad1 and UNC84 domain containing 2 2 2
MIRT654389 RBM12B RNA binding motif protein 12B 2 2
MIRT655637 OLFML2A olfactomedin like 2A 2 2
MIRT655729 NRXN3 neurexin 3 2 2
MIRT658171 FCHSD1 FCH and double SH3 domains 1 2 2
MIRT660937 ACOX1 acyl-CoA oxidase 1 2 2
MIRT662112 CERKL ceramide kinase like 2 2
MIRT662302 MPV17L MPV17 mitochondrial inner membrane protein like 2 2
MIRT662993 TMEM59 transmembrane protein 59 2 2
MIRT663071 SFR1 SWI5 dependent homologous recombination repair protein 1 2 2
MIRT663704 ABHD17B abhydrolase domain containing 17B 2 2
MIRT663864 MUC20 mucin 20, cell surface associated 2 2
MIRT665185 HAUS5 HAUS augmin like complex subunit 5 2 4
MIRT665402 WEE1 WEE1 G2 checkpoint kinase 2 2
MIRT665688 TNPO3 transportin 3 2 2
MIRT665795 TMEM170A transmembrane protein 170A 2 2
MIRT665847 TIAL1 TIA1 cytotoxic granule associated RNA binding protein like 1 2 2
MIRT666999 PDPN podoplanin 2 2
MIRT667598 LIPC lipase C, hepatic type 2 2
MIRT668576 ELMSAN1 ELM2 and Myb/SANT domain containing 1 2 4
MIRT670960 UGGT1 UDP-glucose glycoprotein glucosyltransferase 1 2 2
MIRT671187 ZNF891 zinc finger protein 891 2 2
MIRT671739 ZNF451 zinc finger protein 451 2 2
MIRT672745 ZNF585B zinc finger protein 585B 2 4
MIRT673446 ZNF583 zinc finger protein 583 2 2
MIRT679843 GPR75 G protein-coupled receptor 75 2 2
MIRT680556 ZNF584 zinc finger protein 584 2 2
MIRT680661 C1orf210 chromosome 1 open reading frame 210 2 2
MIRT681285 RFC2 replication factor C subunit 2 2 2
MIRT683264 ZNF329 zinc finger protein 329 2 2
MIRT684519 C1orf174 chromosome 1 open reading frame 174 2 2
MIRT685065 GEMIN4 gem nuclear organelle associated protein 4 2 2
MIRT685157 DTWD2 DTW domain containing 2 2 2
MIRT685168 ERCC1 ERCC excision repair 1, endonuclease non-catalytic subunit 2 2
MIRT685470 CACNG8 calcium voltage-gated channel auxiliary subunit gamma 8 2 2
MIRT686001 NEK4 NIMA related kinase 4 2 2
MIRT687027 RNF24 ring finger protein 24 2 2
MIRT687543 MOB1B MOB kinase activator 1B 2 2
MIRT687592 MANEAL mannosidase endo-alpha like 2 2
MIRT687753 KIAA1328 KIAA1328 2 2
MIRT688050 GLUL glutamate-ammonia ligase 2 2
MIRT688374 ENPP1 ectonucleotide pyrophosphatase/phosphodiesterase 1 2 2
MIRT688802 CBFA2T3 CBFA2/RUNX1 translocation partner 3 2 2
MIRT688957 ATXN3 ataxin 3 2 2
MIRT689281 C5AR2 complement component 5a receptor 2 2 2
MIRT689988 NNMT nicotinamide N-methyltransferase 2 2
MIRT689997 MMP17 matrix metallopeptidase 17 2 2
MIRT690014 LUZP2 leucine zipper protein 2 2 2
MIRT690161 ELP3 elongator acetyltransferase complex subunit 3 2 2
MIRT690379 ZSWIM7 zinc finger SWIM-type containing 7 2 2
MIRT690563 MICA MHC class I polypeptide-related sequence A 2 2
MIRT691891 EVC EvC ciliary complex subunit 1 2 2
MIRT693107 SCNM1 sodium channel modifier 1 2 2
MIRT693832 ZFP64 ZFP64 zinc finger protein 2 2
MIRT694105 ZNF446 zinc finger protein 446 2 2
MIRT694179 ZNF486 zinc finger protein 486 2 2
MIRT694475 LRTOMT leucine rich transmembrane and O-methyltransferase domain containing 2 2
MIRT694998 GGA2 golgi associated, gamma adaptin ear containing, ARF binding protein 2 2 2
MIRT695031 ALG10B ALG10B, alpha-1,2-glucosyltransferase 2 2
MIRT695163 TCTN2 tectonic family member 2 2 2
MIRT695525 SLC25A34 solute carrier family 25 member 34 2 2
MIRT696067 ZNF264 zinc finger protein 264 2 2
MIRT696616 CRIPT CXXC repeat containing interactor of PDZ3 domain 2 2
MIRT696661 AGXT2 alanine--glyoxylate aminotransferase 2 2 2
MIRT696705 PNPO pyridoxamine 5'-phosphate oxidase 2 2
MIRT697173 INMT indolethylamine N-methyltransferase 2 2
MIRT697303 ZNF652 zinc finger protein 652 2 2
MIRT697491 ZBTB8B zinc finger and BTB domain containing 8B 2 2
MIRT698657 TERF2 telomeric repeat binding factor 2 2 2
MIRT699042 SOAT1 sterol O-acyltransferase 1 2 2
MIRT699185 SLX4IP SLX4 interacting protein 2 2
MIRT699598 SHOC2 SHOC2, leucine rich repeat scaffold protein 2 2
MIRT700150 RNF115 ring finger protein 115 2 2
MIRT700720 PNO1 partner of NOB1 homolog 2 2
MIRT700870 PER2 period circadian clock 2 2 2
MIRT700912 PDXK pyridoxal kinase 2 2
MIRT701095 PAPOLG poly(A) polymerase gamma 2 2
MIRT701194 OTUD3 OTU deubiquitinase 3 2 2
MIRT702203 LPP LIM domain containing preferred translocation partner in lipoma 2 2
MIRT702272 LHX4 LIM homeobox 4 2 3
MIRT703125 GPRC5A G protein-coupled receptor class C group 5 member A 2 2
MIRT703252 GNS glucosamine (N-acetyl)-6-sulfatase 2 2
MIRT703262 GNL3L G protein nucleolar 3 like 2 2
MIRT703440 FYTTD1 forty-two-three domain containing 1 2 2
MIRT704321 DCUN1D5 defective in cullin neddylation 1 domain containing 5 2 2
MIRT704393 CTSS cathepsin S 2 2
MIRT704483 CPT1A carnitine palmitoyltransferase 1A 2 2
MIRT704880 CCSER2 coiled-coil serine rich protein 2 2 2
MIRT704926 CCDC36 coiled-coil domain containing 36 2 2
MIRT705175 BZW1 basic leucine zipper and W2 domains 1 2 2
MIRT706561 EIF2AK2 eukaryotic translation initiation factor 2 alpha kinase 2 2 2
MIRT707048 TRPV2 transient receptor potential cation channel subfamily V member 2 2 2
MIRT711497 PGD phosphogluconate dehydrogenase 2 2
MIRT716644 EPGN epithelial mitogen 2 2
MIRT720083 TNRC6B trinucleotide repeat containing 6B 2 2
MIRT722288 PMPCA peptidase, mitochondrial processing alpha subunit 2 2
MIRT725468 GRAP2 GRB2-related adaptor protein 2 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4433a Dabrafenib 44462760 NSC764134 approved resistant cell line (A375)
hsa-mir-4433a Paclitaxel 36314 NSC125973 approved resistant cell line (W1)
hsa-mir-4433a Vincristine 5978 approved resistant cell line (W1)
hsa-mir-4433a Cisplatin 5460033 NSC119875 approved resistant cell line (BxPC3)
hsa-miR-4433a-3p Platinum 23939 sensitive High Ovarian Cancer tissue
hsa-miR-4433a-3p Imatinib 5291 NSC743414 approved sensitive High Gastrointestinal Stromal Tumor cell line (882R-NC, 882R-OE, 882R-KD)
hsa-miR-4433a-3p Cisplatin 5460033 NSC119875 approved resistant High Pancreatic Cancer cell line (BxPC-3)
hsa-miR-4433a-3p Paclitaxel 36314 NSC125973 approved resistant High Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-4433a-3p Imatinib 5291 NSC743414 approved sensitive High Chronic Myelogenous Leukemia tissue
hsa-miR-4433a-3p Cisplatin 5460033 NSC119875 approved resistant High Ovarian Cancer cell line (A2780CIS)
hsa-miR-4433a-3p Cisplatin 5460033 NSC119875 approved sensitive cell line (CAL-27) (mitochondrial RNA)
hsa-miR-4433a-3p Paclitaxel 36314 NSC125973 approved resistant cell line (BAS)
hsa-miR-4433a-3p Cisplatin 5460033 NSC119875 approved resistant cell line (CP20)
hsa-miR-4433a-3p Cisplatin 5460033 NSC119875 approved resistant cell line (CIS)
hsa-miR-4433a-3p Neoadjuvant chemotherapy sensitive tissue (breast cancer)
hsa-miR-4433a-3p Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)
hsa-miR-4433a-3p Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)

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