pre-miRNA Information
pre-miRNA hsa-mir-4753   
Genomic Coordinates chr1: 235190034 - 235190116
Description Homo sapiens miR-4753 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4753-5p
Sequence 10| CAAGGCCAAAGGAAGAGAACAG |31
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs547986869 3 dbSNP
rs1007787337 7 dbSNP
rs1224263160 12 dbSNP
rs1256249071 13 dbSNP
rs1484015538 14 dbSNP
rs535678819 19 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol CNGA2   
Synonyms CNCA, CNCA1, CNG2, OCNC1, OCNCALPHA, OCNCa
Description cyclic nucleotide gated channel alpha 2
Transcript NM_005140   
Expression
Putative miRNA Targets on CNGA2
3'UTR of CNGA2
(miRNA target sites are highlighted)
>CNGA2|NM_005140|3'UTR
   1 GACCTGGGGCCCAACTGCCTCTCCAGCATTGGCCTTGGCCTTGATCCCAGAAGCTAGAGGAGCTATTTAGATCTCCGGAT
  81 TTACATGCATTACCCTCATGTTCCCTGAATTCTCCCAAAAGCCTCTCTGACCCTGGGTTTTTGGCCTAAACATCCAAGAT
 161 TCCGCCTCCAAGTTTAGCCCAAGTTTGTGGAGAGTACAGACTGCGTTGGCTGGGCTTCCGAGAGCTTCGGCCTGCCTAAG
 241 TCTGAGGAAGGGAGAAGGGGGCAGCTGTCTGCCAGGAGTCTGGCTCTTTTGCTCATAGCGACCCCTCCCTTGGTTCTGGC
 321 CCCCGCTTTTTCTAACATGTCTTCTGAATGCTTCCTTTTTCCCTGCACACGCATGTACTTCGAGCACCGACTATAGACAT
 401 TTAGATCAGGTACCCAGTGTCTGTCTCCCACGACAGGCCAAGGGATGTGATAGTGTTGTTGGGAGGGGGTAGAAGGGTGC
 481 CCACTCAGGGTTATTCCTCCCCCATCAGATGCTCCAGCTCCTGCTCTGTAGATTGATCTGTCTGTCTGCTAGCCAGGATG
 561 GGATCTCTGCAGTCTTCTGTGGACCAAGGAGATAGACCCGGCTTCTGGTCCATTCCAGGGCAGGGAGTGAGGAGCAAGGC
 641 AACTGGCATTTGTCCACCTGGAGACAGAGGTAGCTGGCAGCTCTGGCCATCCCTCCCCTATGAAGAGCATTCCTAAAGAG
 721 GCCCTGAAAACTGCTGGTGGTGGGGAGACTCTTCAAGTTAATATCTGCATTAGAGGCACCTATGAGTTAAAAAATTCCTT
 801 TGAACTATCTGTGGTTATTGCTCAACTCTGCTGTTCACATTCTCTCCCTCCCTTCATTAATCCCTCTCTTTTCACCATAT
 881 GGTTGTCCCTCAGTATTCATCAGGGATTGCTTTCAGGAGTCCCCATGGATACCAAAATTTGTGGATGTTCAAGTCCCTGA
 961 TACAAAATGACGTCGTATTTGAATATAACCTATGCACATCCTCCCATACTTTAAGTCATCTCTCGATTATTTGTAACACC
1041 TAATACAATGTCAGTGCTATGTAAAT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' gacaaGAGAAG--GAAACCGGAAc 5'
               |||| |  | |||||||| 
Target 5' actgcCTCTCCAGCATTGGCCTTg 3'
14 - 37 159.00 -21.80
2
miRNA  3' gacaAGAGA-----A---GGAAACCGGAac 5'
              |||||     |   ::||||||||  
Target 5' agccTCTCTGACCCTGGGTTTTTGGCCTaa 3'
120 - 149 134.00 -15.50
3
miRNA  3' gaCAAGAGAAGG-----AAACCG-GAAc 5'
            | | ||| ||     | |||| ||| 
Target 5' caGCTGTCTGCCAGGAGTCTGGCTCTTt 3'
262 - 289 120.00 -12.50
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1160170580 10 dbSNP
rs1453871978 14 dbSNP
rs1393229866 15 dbSNP
rs373377455 16 dbSNP
rs944254144 22 dbSNP
rs201322634 24 dbSNP
rs201215466 26 dbSNP
rs372415417 26 dbSNP
rs759697367 26 dbSNP
rs1403769740 27 dbSNP
rs747495786 28 dbSNP
rs200284228 29 dbSNP
rs771487965 29 dbSNP
rs370788911 30 dbSNP
rs780752593 30 dbSNP
rs1327781115 34 dbSNP
rs1342421887 35 dbSNP
rs971091109 46 dbSNP
rs373858996 47 dbSNP
rs980142808 64 dbSNP
rs1276257175 69 dbSNP
rs1372018579 77 dbSNP
rs183601214 78 dbSNP
rs771682591 79 dbSNP
rs1299372351 86 dbSNP
rs1311522218 90 dbSNP
rs1413046024 93 dbSNP
rs1401727105 100 dbSNP
rs147042412 105 dbSNP
rs1479059062 113 dbSNP
rs904263781 132 dbSNP
rs1326772277 146 dbSNP
rs777735927 147 dbSNP
rs973970464 148 dbSNP
rs746450347 164 dbSNP
rs918454283 165 dbSNP
rs746772169 188 dbSNP
rs1031301345 198 dbSNP
rs5969818 200 dbSNP
rs1469585190 204 dbSNP
rs1014565545 205 dbSNP
rs1022864636 206 dbSNP
rs780306810 220 dbSNP
rs113307189 221 dbSNP
rs1279902246 229 dbSNP
rs944736705 230 dbSNP
rs149174319 233 dbSNP
rs1335138383 238 dbSNP
rs768930780 241 dbSNP
rs1414865793 251 dbSNP
rs1416317172 253 dbSNP
rs763400874 259 dbSNP
rs1038986697 260 dbSNP
rs1170040223 261 dbSNP
rs774534656 263 dbSNP
rs1466664458 266 dbSNP
rs952759420 271 dbSNP
rs900492070 277 dbSNP
rs1000433504 283 dbSNP
rs41302160 292 dbSNP
rs1438948971 300 dbSNP
rs1291839299 301 dbSNP
rs879372 304 dbSNP
rs1054627560 313 dbSNP
rs1255674082 321 dbSNP
rs773896768 325 dbSNP
rs762079118 326 dbSNP
rs111389709 329 dbSNP
rs73640115 354 dbSNP
rs1273639921 356 dbSNP
rs750657766 360 dbSNP
rs150893838 364 dbSNP
rs904691817 371 dbSNP
rs934363082 372 dbSNP
rs1330814119 381 dbSNP
rs1001228131 382 dbSNP
rs1052867351 383 dbSNP
rs895580338 385 dbSNP
rs766476643 389 dbSNP
rs962361110 390 dbSNP
rs1254512752 391 dbSNP
rs1446120464 392 dbSNP
rs1235950088 393 dbSNP
rs1471935677 400 dbSNP
rs138480757 404 dbSNP
rs974022985 406 dbSNP
rs918466094 420 dbSNP
rs951252722 427 dbSNP
rs905780000 432 dbSNP
rs142321306 433 dbSNP
rs145943840 447 dbSNP
rs1308835260 454 dbSNP
rs950567582 459 dbSNP
rs1001739712 462 dbSNP
rs1349542482 467 dbSNP
rs982807635 470 dbSNP
rs1291803439 472 dbSNP
rs1436474561 500 dbSNP
rs879371 506 dbSNP
rs1326000009 510 dbSNP
rs1418272947 513 dbSNP
rs1380030058 518 dbSNP
rs1310002721 521 dbSNP
rs1439862622 527 dbSNP
rs987009308 532 dbSNP
rs1188946622 537 dbSNP
rs1488139497 539 dbSNP
rs1247192403 544 dbSNP
rs1224567900 545 dbSNP
rs1487205332 553 dbSNP
rs758215071 555 dbSNP
rs1217642969 566 dbSNP
rs965873878 574 dbSNP
rs1409184698 594 dbSNP
rs1293644812 599 dbSNP
rs149699381 600 dbSNP
rs921301863 601 dbSNP
rs1218694755 611 dbSNP
rs932877235 621 dbSNP
rs979015471 625 dbSNP
rs926079953 626 dbSNP
rs5925021 630 dbSNP
rs1299171316 645 dbSNP
rs1053310178 656 dbSNP
rs749779127 658 dbSNP
rs1271934097 671 dbSNP
rs944810415 675 dbSNP
rs188328217 683 dbSNP
rs774928171 683 dbSNP
rs949881115 688 dbSNP
rs1476768673 690 dbSNP
rs1258867929 706 dbSNP
rs757939877 707 dbSNP
rs1475920914 715 dbSNP
rs1044028536 723 dbSNP
rs921970372 726 dbSNP
rs376390986 728 dbSNP
rs1254503281 730 dbSNP
rs994685921 736 dbSNP
rs1312850344 742 dbSNP
rs148445403 745 dbSNP
rs886381427 756 dbSNP
rs1429394043 760 dbSNP
rs1340443874 764 dbSNP
rs751507238 773 dbSNP
rs767190642 780 dbSNP
rs867062639 798 dbSNP
rs1384252526 805 dbSNP
rs1377366811 806 dbSNP
rs1300749573 808 dbSNP
rs1465175560 810 dbSNP
rs181193441 818 dbSNP
rs1177955305 820 dbSNP
rs41311688 824 dbSNP
rs1313359354 839 dbSNP
rs1422797594 852 dbSNP
rs766428810 858 dbSNP
rs753926496 861 dbSNP
rs1430113002 862 dbSNP
rs1046296611 869 dbSNP
rs965488652 877 dbSNP
rs1181508084 878 dbSNP
rs1483692678 879 dbSNP
rs1245111653 886 dbSNP
rs1196792459 888 dbSNP
rs1001316632 900 dbSNP
rs998677875 910 dbSNP
rs1028287970 911 dbSNP
rs1320300490 917 dbSNP
rs759516163 920 dbSNP
rs954038426 929 dbSNP
rs1383068227 946 dbSNP
rs1362209924 965 dbSNP
rs1230225906 972 dbSNP
rs978984286 973 dbSNP
rs926182072 975 dbSNP
rs1463407780 976 dbSNP
rs1340798329 993 dbSNP
rs1163799607 1008 dbSNP
rs1424654807 1022 dbSNP
rs956310850 1025 dbSNP
rs370819184 1038 dbSNP
rs1234419659 1041 dbSNP
rs372457923 1058 dbSNP
rs988978506 1061 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions LCL-BAC
Disease MIMAT0019890
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM1020023. RNA binding protein: AGO2. Condition:EBV B95-8-infected ...

- Skalsky RL; Corcoran DL; Gottwein E; Frank et al., 2012, PLoS pathogens.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' gacaagagaagGAAACCGGAAc 5'
                     | |||||||| 
Target 5' -------uuggCCUUGGCCUUg 3'
1 - 15
Article - Skalsky RL; Corcoran DL; Gottwein E; Frank et al.
- PLoS pathogens, 2012
Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus linked to a number of B cell cancers and lymphoproliferative disorders. During latent infection, EBV expresses 25 viral pre-microRNAs (miRNAs) and induces the expression of specific host miRNAs, such as miR-155 and miR-21, which potentially play a role in viral oncogenesis. To date, only a limited number of EBV miRNA targets have been identified; thus, the role of EBV miRNAs in viral pathogenesis and/or lymphomagenesis is not well defined. Here, we used photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) combined with deep sequencing and computational analysis to comprehensively examine the viral and cellular miRNA targetome in EBV strain B95-8-infected lymphoblastoid cell lines (LCLs). We identified 7,827 miRNA-interaction sites in 3,492 cellular 3'UTRs. 531 of these sites contained seed matches to viral miRNAs. 24 PAR-CLIP-identified miRNA:3'UTR interactions were confirmed by reporter assays. Our results reveal that EBV miRNAs predominantly target cellular transcripts during latent infection, thereby manipulating the host environment. Furthermore, targets of EBV miRNAs are involved in multiple cellular processes that are directly relevant to viral infection, including innate immunity, cell survival, and cell proliferation. Finally, we present evidence that myc-regulated host miRNAs from the miR-17/92 cluster can regulate latent viral gene expression. This comprehensive survey of the miRNA targetome in EBV-infected B cells represents a key step towards defining the functions of EBV-encoded miRNAs, and potentially, identifying novel therapeutic targets for EBV-associated malignancies.
LinkOut: [PMID: 22291592]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions LCL-BAC-D2
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM1133252. RNA binding protein: AGO2. Condition:Untreated ...

- Majoros WH; Lekprasert P; Mukherjee N; et al., 2013, Nature methods.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' gacaagagaagGAAACCGGAAc 5'
                     | |||||||| 
Target 5' -------uuggCCUUGGCCUUg 3'
1 - 15
Article - Majoros WH; Lekprasert P; Mukherjee N; et al.
- Nature methods, 2013
High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute proteins can pinpoint a microRNA (miRNA) target site within tens of bases but leaves the identity of the miRNA unresolved. A flexible computational framework, microMUMMIE, integrates sequence with cross-linking features and reliably identifies the miRNA family involved in each binding event. It considerably outperforms sequence-only approaches and quantifies the prevalence of noncanonical binding modes.
LinkOut: [PMID: 23708386]
CLIP-seq Support 1 for dataset GSM1020023
Method / RBP PAR-CLIP / AGO2
Cell line / Condition LCL-BAC / EBV B95-8-infected, 4-thiouridine, RNase T1
Location of target site ENST00000329903.4 | 3UTR | UUGGCCUUGGCCUUGAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 22291592 / GSE41437
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1133252
Method / RBP PAR-CLIP / AGO2
Cell line / Condition LCL-BAC-D2 / Untreated
Location of target site ENST00000329903.4 | 3UTR | UUGGCCUUGGCCUUGAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23708386 / GSE46611
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
94 hsa-miR-4753-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT064916 ZBTB18 zinc finger and BTB domain containing 18 2 2
MIRT161166 SLC25A36 solute carrier family 25 member 36 2 2
MIRT285542 CDT1 chromatin licensing and DNA replication factor 1 2 2
MIRT308266 LRIG1 leucine rich repeats and immunoglobulin like domains 1 2 2
MIRT311425 LMNB1 lamin B1 2 2
MIRT373989 PEBP1 phosphatidylethanolamine binding protein 1 2 4
MIRT383141 CRY2 cryptochrome circadian clock 2 2 2
MIRT405243 ADIPOR2 adiponectin receptor 2 2 2
MIRT441620 ROCK1 Rho associated coiled-coil containing protein kinase 1 2 6
MIRT441789 SRPK1 SRSF protein kinase 1 2 2
MIRT441804 NOC3L NOC3 like DNA replication regulator 2 2
MIRT441832 ALG14 ALG14, UDP-N-acetylglucosaminyltransferase subunit 2 2
MIRT442005 NDUFV3 NADH:ubiquinone oxidoreductase subunit V3 2 2
MIRT442411 LIMD1 LIM domains containing 1 2 2
MIRT442708 UBE4B ubiquitination factor E4B 2 2
MIRT442714 TNRC6B trinucleotide repeat containing 6B 2 2
MIRT442738 SERINC5 serine incorporator 5 2 2
MIRT442793 CEP170 centrosomal protein 170 2 2
MIRT442984 ZNF736 zinc finger protein 736 2 2
MIRT443055 THRB thyroid hormone receptor beta 2 2
MIRT443288 ZC3H12A zinc finger CCCH-type containing 12A 2 2
MIRT443325 SLC35G1 solute carrier family 35 member G1 2 2
MIRT443331 OCRL OCRL, inositol polyphosphate-5-phosphatase 2 2
MIRT443593 ZNF439 zinc finger protein 439 2 4
MIRT443696 KCNN3 potassium calcium-activated channel subfamily N member 3 2 2
MIRT443748 ELL2 elongation factor for RNA polymerase II 2 2 2
MIRT443866 HDLBP high density lipoprotein binding protein 2 2
MIRT461185 LTBP2 latent transforming growth factor beta binding protein 2 2 2
MIRT464074 WAC WW domain containing adaptor with coiled-coil 2 2
MIRT468738 SDC4 syndecan 4 2 2
MIRT470540 COASY Coenzyme A synthase 2 2
MIRT476458 GBA2 glucosylceramidase beta 2 2 2
MIRT479470 CDK6 cyclin dependent kinase 6 2 2
MIRT486351 TACC2 transforming acidic coiled-coil containing protein 2 2 8
MIRT495126 CXorf67 chromosome X open reading frame 67 2 2
MIRT495166 CNGA2 cyclic nucleotide gated channel alpha 2 2 4
MIRT495432 ATG7 autophagy related 7 2 2
MIRT495922 FBXO41 F-box protein 41 2 2
MIRT496572 DGCR6L DiGeorge syndrome critical region gene 6 like 2 2
MIRT498277 POFUT1 protein O-fucosyltransferase 1 2 2
MIRT498431 DDX39A DExD-box helicase 39A 2 2
MIRT530149 HADHB hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit 2 2
MIRT530313 TNFRSF10D TNF receptor superfamily member 10d 2 2
MIRT530880 TRUB1 TruB pseudouridine synthase family member 1 2 4
MIRT531177 ZNF626 zinc finger protein 626 2 2
MIRT533395 TYRP1 tyrosinase related protein 1 2 2
MIRT533709 TMEM64 transmembrane protein 64 2 2
MIRT533954 TAF1D TATA-box binding protein associated factor, RNA polymerase I subunit D 2 2
MIRT535616 NSD1 nuclear receptor binding SET domain protein 1 2 2
MIRT539472 ADARB2 adenosine deaminase, RNA specific B2 (inactive) 2 2
MIRT542878 NR6A1 nuclear receptor subfamily 6 group A member 1 2 2
MIRT559077 C19orf47 chromosome 19 open reading frame 47 2 2
MIRT559465 ARPP19 cAMP regulated phosphoprotein 19 2 2
MIRT561233 ZNF772 zinc finger protein 772 2 2
MIRT563477 POLE3 DNA polymerase epsilon 3, accessory subunit 2 2
MIRT563995 SLFN11 schlafen family member 11 2 2
MIRT564222 SDE2 SDE2 telomere maintenance homolog 2 2
MIRT566017 RHOA ras homolog family member A 2 2
MIRT566028 RFX1 regulatory factor X1 2 2
MIRT566591 NUFIP2 NUFIP2, FMR1 interacting protein 2 2 2
MIRT567874 CTDSP1 CTD small phosphatase 1 2 2
MIRT568552 AKT2 AKT serine/threonine kinase 2 2 2
MIRT569357 EFHC1 EF-hand domain containing 1 2 2
MIRT569973 DNAAF2 dynein axonemal assembly factor 2 2 2
MIRT614423 ZNF440 zinc finger protein 440 2 2
MIRT628831 SLC25A34 solute carrier family 25 member 34 2 2
MIRT630142 ZFYVE9 zinc finger FYVE-type containing 9 2 2
MIRT634479 PAFAH1B2 platelet activating factor acetylhydrolase 1b catalytic subunit 2 2 2
MIRT634498 OR7D2 olfactory receptor family 7 subfamily D member 2 2 2
MIRT637394 R3HDM2 R3H domain containing 2 2 2
MIRT641840 TCF7L2 transcription factor 7 like 2 2 2
MIRT644397 CDKL1 cyclin dependent kinase like 1 2 2
MIRT644888 C2orf50 chromosome 2 open reading frame 50 2 2
MIRT647124 ZNF446 zinc finger protein 446 2 2
MIRT647420 SSTR3 somatostatin receptor 3 2 2
MIRT650297 PYCARD PYD and CARD domain containing 2 2
MIRT655488 PAK3 p21 (RAC1) activated kinase 3 2 2
MIRT658185 FBXO9 F-box protein 9 2 2
MIRT660931 ADAM19 ADAM metallopeptidase domain 19 2 2
MIRT665317 ZBTB3 zinc finger and BTB domain containing 3 2 2
MIRT670350 C1orf106 chromosome 1 open reading frame 106 2 4
MIRT670823 NICN1 nicolin 1 2 2
MIRT671825 TRPM6 transient receptor potential cation channel subfamily M member 6 2 2
MIRT672732 NETO2 neuropilin and tolloid like 2 2 2
MIRT674841 GLRX2 glutaredoxin 2 2 2
MIRT675930 CYP51A1 cytochrome P450 family 51 subfamily A member 1 2 2
MIRT686786 AZF1 azoospermia factor 1 2 2
MIRT697723 USP8 ubiquitin specific peptidase 8 2 2
MIRT702357 KLHL26 kelch like family member 26 2 2
MIRT704361 DBR1 debranching RNA lariats 1 2 2
MIRT709242 RANGAP1 Ran GTPase activating protein 1 2 2
MIRT714433 SNED1 sushi, nidogen and EGF like domains 1 2 2
MIRT717000 ARL6IP4 ADP ribosylation factor like GTPase 6 interacting protein 4 2 2
MIRT720875 ADCY5 adenylate cyclase 5 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4753 Cisplatin 5460033 NSC119875 approved sensitive cell line (A2780)
hsa-miR-4753-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-4753-5p Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-4753-5p Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)
hsa-miR-4753-5p Cisplatin 5460033 NSC119875 approved resistant cell line (A2780)
hsa-miR-4753-5p Platinum 23939 resistant tissue
hsa-miR-4753-5p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-4753-5p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (100 ng/ml)
hsa-miR-4753-5p Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR4)

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