pre-miRNA Information | |
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pre-miRNA | hsa-mir-4640 |
Genomic Coordinates | chr6: 30890883 - 30890972 |
Description | Homo sapiens miR-4640 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |||||||||||||||||||||||||
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Mature miRNA | hsa-miR-4640-5p | ||||||||||||||||||||||||
Sequence | 9| UGGGCCAGGGAGCAGCUGGUGGG |31 | ||||||||||||||||||||||||
Evidence | Experimental | ||||||||||||||||||||||||
Experiments | Illumina | ||||||||||||||||||||||||
SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
Circulating MicroRNA Expression Profiling |
Biomarker Information |
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Gene Information | |||||||||||||||||||||
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Gene Symbol | NPHP3 | ||||||||||||||||||||
Synonyms | CFAP31, MKS7, NPH3, RHPD, RHPD1, SLSN3 | ||||||||||||||||||||
Description | nephrocystin 3 | ||||||||||||||||||||
Transcript | NM_153240 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on NPHP3 | |||||||||||||||||||||
3'UTR of NPHP3 (miRNA target sites are highlighted) |
>NPHP3|NM_153240|3'UTR 1 TAGCAGCAGTTAGAATTCTTTGCAAATGTACCTTAAGACAAAATAATTAAACATTTGGAACATTTGAATTTGAAACTTTA 81 AAAAAATGTTGTACGAAATTTTACTACGTGTGATTTAACTGCTATTTGTATGAAGTTGTATTGGATTACATTAAGTTGGA 161 ATTGTGATTATGTCTGTTTTAGTTGTTTAAAAGAATTTTCCTATTATATGGTATCCAAGGATGTAGACACATTAGAATTA 241 TAAGAAGACATGAGGAGCAAATCATGAAGAGCGGATTGGTCTTTGTTCAACAAGAGCTGGCAGAGTAGTTAAGACAAGGA 321 GTTCAAAAATTCCATGAATCTTGGCCAGGCATGGTGGCTCATGCCTGTAATCCCACCACTTTGGGAGGGTGATGCAGGAG 401 GATCACTTGAGGCCAGGAGTTGGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAAAAAAT 481 TAGCTGGACCTGGTGGCGCATGCCTGTAATCCTAGCTACTCAGGAGGCTGAGGCATGAGAATCGCTTGAACTCAGCAAGT 561 GGAGGTTGCTATGAGCTGAGATTGTGCCAGTTCATTCCACCCTGGGCAACAGGGTGAGACTCTGTCTCAAAAAAAAAAAA 641 AAAAAAAAAAAGAATTCCATGATTCTTTTGAGCTCTCAAGAGCTACTTTAGCCTTGCCATTCCTCTTGCTTTTCAGCAGT 721 AGTCTGGCCCAATTCTCTACCTCAAAGGAAAGTTTCAAGGATTAAAGGGTTAATGTTTATGAACGATTCAAATTGTCTTC 801 AGTGAAACACATTAGTCACAAGAATCCCTGATTGCTGCACTTTAAAAAATTCTTTACCTTTTCCTTGAGCAGATTGGTCC 881 AGTCCCTCTGCTTTCTGTTCTGTCTACATTGTGTATTATTTATTACTATATAGTATATGTATCTGCATAATGTCTGAAGT 961 CATTAATGCAGTAACAGGTCAAGCTCTTTAGCATTCTTTGACTTTTCCCAAAATATAGATATGACATTCTCCTTTATCTT 1041 CCTTCTTAGATGGCCTAAATCTGTCTCTTCATTTACCTCAAACTCAAATTAGTATGCCATTTTCATACTGAAAATTAAAT 1121 ATTTTTATGGATTTTATAAATATTGCAACATTTTAGATATTGTTTTGGACCATTTGTGTATTTATATATAATAAAATCAT 1201 CAATTTAATACTTGTAAGATCCTAACATGTATTGATTGTAAAAGTGAAATATGTAATAGCCACACTTCACTGTTTTAAAA 1281 TAAAAGTGCAATTTCAAGTTCAAAAAAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | MCF7 | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
PAR-CLIP data was present in SRR1045082. RNA binding protein: AGO2. Condition:Untreated
... - Farazi TA; Ten Hoeve JJ; Brown M; et al., 2014, Genome biology. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Farazi TA; Ten Hoeve JJ; Brown M; et al. - Genome biology, 2014
BACKGROUND: Various microRNAs (miRNAs) are up- or downregulated in tumors. However, the repression of cognate miRNA targets responsible for the phenotypic effects of this dysregulation in patients remains largely unexplored. To define miRNA targets and associated pathways, together with their relationship to outcome in breast cancer, we integrated patient-paired miRNA-mRNA expression data with a set of validated miRNA targets and pathway inference. RESULTS: To generate a biochemically-validated set of miRNA-binding sites, we performed argonaute-2 photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (AGO2-PAR-CLIP) in MCF7 cells. We then defined putative miRNA-target interactions using a computational model, which ranked and selected additional TargetScan-predicted interactions based on features of our AGO2-PAR-CLIP binding-site data. We subselected modeled interactions according to the abundance of their constituent miRNA and mRNA transcripts in tumors, and we took advantage of the variability of miRNA expression within molecular subtypes to detect miRNA repression. Interestingly, our data suggest that miRNA families control subtype-specific pathways; for example, miR-17, miR-19a, miR-25, and miR-200b show high miRNA regulatory activity in the triple-negative, basal-like subtype, whereas miR-22 and miR-24 do so in the HER2 subtype. An independent dataset validated our findings for miR-17 and miR-25, and showed a correlation between the expression levels of miR-182 targets and overall patient survival. Pathway analysis associated miR-17, miR-19a, and miR-200b with leukocyte transendothelial migration. CONCLUSIONS: We combined PAR-CLIP data with patient expression data to predict regulatory miRNAs, revealing potential therapeutic targets and prognostic markers in breast cancer.
LinkOut: [PMID: 24398324]
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CLIP-seq Support 1 for dataset GSM4903833 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / CTL_TD_21_a |
Location of target site | NM_153240 | 3UTR | CCAUGAUUCUUUUGAGCUCUCAAGAGCUACUUUAGCCUUGCCAUUCCUCUUGCUUUUCAGCAGUAGUCUGGCCCAAUUCUCUACCUCAAAGGAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM4903834 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / CTL_TD_21_b |
Location of target site | NM_153240 | 3UTR | UCCAUGAUUCUUUUGAGCUCUCAAGAGCUACUUUAGCCUUGCCAUUCCUCUUGCUUUUCAGCAGUAGUCUGGCCCAAUUCUCUACCUCAAAGGAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset GSM4903835 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / CTL_TD_21_c |
Location of target site | NM_153240 | 3UTR | AUUCCAUGAUUCUUUUGAGCUCUCAAGAGCUACUUUAGCCUUGCCAUUCCUCUUGCUUUUCAGCAGUAGUCUGGCCCAAUUCUCUACCUCAAAGGAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 4 for dataset GSM4903836 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / 124_TD_21_a |
Location of target site | NM_153240 | 3UTR | GAGCUCUCAAGAGCUACUUUAGCCUUGCCAUUCCUCUUGCUUUUCAGCAGUAGUCUGGCCCAAUUCUCUACCUCAAAGGAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 5 for dataset GSM4903837 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / 124_TD_21_b |
Location of target site | NM_153240 | 3UTR | UCAAGAGCUACUUUAGCCUUGCCAUUCCUCUUGCUUUUCAGCAGUAGUCUGGCCCAAUUCUCUACCUCAAAGGAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 6 for dataset SRR1045082 | |
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Method / RBP | PAR-CLIP / AGO2 |
Cell line / Condition | MCF7 / Untreated |
Location of target site | ENST00000326682.8 | 3UTR | CCCAAUUCUCUACCUCAAAG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 24398324 / SRX388831 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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87 hsa-miR-4640-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT100106 | ABT1 | activator of basal transcription 1 | ![]() |
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2 | 8 | ||||||
MIRT248144 | LMBR1L | limb development membrane protein 1 like | ![]() |
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2 | 2 | ||||||
MIRT327062 | KLHL15 | kelch like family member 15 | ![]() |
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2 | 2 | ||||||
MIRT347231 | GATAD2A | GATA zinc finger domain containing 2A | ![]() |
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2 | 2 | ||||||
MIRT450221 | CENPN | centromere protein N | ![]() |
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2 | 2 | ||||||
MIRT451264 | NDUFA11 | NADH:ubiquinone oxidoreductase subunit A11 | ![]() |
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2 | 2 | ||||||
MIRT452701 | C1orf226 | chromosome 1 open reading frame 226 | ![]() |
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2 | 2 | ||||||
MIRT453212 | CERS1 | ceramide synthase 1 | ![]() |
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2 | 2 | ||||||
MIRT454822 | POLR2J3 | RNA polymerase II subunit J3 | ![]() |
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2 | 2 | ||||||
MIRT454883 | RAD50 | RAD50 double strand break repair protein | ![]() |
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2 | 2 | ||||||
MIRT455783 | TAF8 | TATA-box binding protein associated factor 8 | ![]() |
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2 | 2 | ||||||
MIRT455858 | TMEM254 | transmembrane protein 254 | ![]() |
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2 | 2 | ||||||
MIRT457615 | UPK3BL | uroplakin 3B like 1 | ![]() |
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2 | 2 | ||||||
MIRT457822 | ITPRIP | inositol 1,4,5-trisphosphate receptor interacting protein | ![]() |
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2 | 4 | ||||||
MIRT458344 | NOC2L | NOC2 like nucleolar associated transcriptional repressor | ![]() |
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2 | 2 | ||||||
MIRT458376 | ITM2C | integral membrane protein 2C | ![]() |
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2 | 2 | ||||||
MIRT458928 | SAMD4B | sterile alpha motif domain containing 4B | ![]() |
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2 | 2 | ||||||
MIRT459066 | WFIKKN2 | WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 | ![]() |
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2 | 2 | ||||||
MIRT460138 | ASB16 | ankyrin repeat and SOCS box containing 16 | ![]() |
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2 | 2 | ||||||
MIRT460818 | FSTL4 | follistatin like 4 | ![]() |
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2 | 2 | ||||||
MIRT461285 | COX10 | COX10, heme A:farnesyltransferase cytochrome c oxidase assembly factor | ![]() |
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2 | 2 | ||||||
MIRT462738 | EFNB1 | ephrin B1 | ![]() |
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2 | 2 | ||||||
MIRT464556 | UBTF | upstream binding transcription factor, RNA polymerase I | ![]() |
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2 | 2 | ||||||
MIRT477910 | DUSP2 | dual specificity phosphatase 2 | ![]() |
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2 | 2 | ||||||
MIRT479073 | CNNM4 | cyclin and CBS domain divalent metal cation transport mediator 4 | ![]() |
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2 | 4 | ||||||
MIRT479534 | CDC5L | cell division cycle 5 like | ![]() |
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2 | 4 | ||||||
MIRT485628 | EEPD1 | endonuclease/exonuclease/phosphatase family domain containing 1 | ![]() |
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2 | 2 | ||||||
MIRT489896 | PPIC | peptidylprolyl isomerase C | ![]() |
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2 | 4 | ||||||
MIRT490737 | SRCIN1 | SRC kinase signaling inhibitor 1 | ![]() |
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2 | 2 | ||||||
MIRT491201 | MLLT1 | MLLT1, super elongation complex subunit | ![]() |
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2 | 4 | ||||||
MIRT492681 | PHYHIP | phytanoyl-CoA 2-hydroxylase interacting protein | ![]() |
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2 | 2 | ||||||
MIRT494593 | ATG7 | autophagy related 7 | ![]() |
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2 | 2 | ||||||
MIRT495061 | PADI3 | peptidyl arginine deiminase 3 | ![]() |
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2 | 4 | ||||||
MIRT496623 | TMEM67 | transmembrane protein 67 | ![]() |
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2 | 2 | ||||||
MIRT497177 | ZBTB40 | zinc finger and BTB domain containing 40 | ![]() |
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2 | 2 | ||||||
MIRT498217 | TLN2 | talin 2 | ![]() |
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2 | 2 | ||||||
MIRT498306 | BCL11B | B-cell CLL/lymphoma 11B | ![]() |
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2 | 2 | ||||||
MIRT499668 | NPHP3 | nephrocystin 3 | ![]() |
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2 | 2 | ||||||
MIRT508080 | ANKRD52 | ankyrin repeat domain 52 | ![]() |
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2 | 2 | ||||||
MIRT509707 | ANKRD23 | ankyrin repeat domain 23 | ![]() |
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2 | 2 | ||||||
MIRT509841 | FOS | Fos proto-oncogene, AP-1 transcription factor subunit | ![]() |
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2 | 2 | ||||||
MIRT512814 | ARRDC2 | arrestin domain containing 2 | ![]() |
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2 | 2 | ||||||
MIRT513353 | SLIT1 | slit guidance ligand 1 | ![]() |
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2 | 2 | ||||||
MIRT514293 | FXYD5 | FXYD domain containing ion transport regulator 5 | ![]() |
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2 | 2 | ||||||
MIRT516598 | FAM89A | family with sequence similarity 89 member A | ![]() |
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2 | 4 | ||||||
MIRT516616 | DARS2 | aspartyl-tRNA synthetase 2, mitochondrial | ![]() |
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2 | 2 | ||||||
MIRT518348 | CCL5 | C-C motif chemokine ligand 5 | ![]() |
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2 | 2 | ||||||
MIRT520130 | WSB1 | WD repeat and SOCS box containing 1 | ![]() |
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2 | 2 | ||||||
MIRT522072 | ORAI2 | ORAI calcium release-activated calcium modulator 2 | ![]() |
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2 | 2 | ||||||
MIRT526461 | OSBPL5 | oxysterol binding protein like 5 | ![]() |
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2 | 2 | ||||||
MIRT528278 | MBL2 | mannose binding lectin 2 | ![]() |
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2 | 2 | ||||||
MIRT534844 | RAB15 | RAB15, member RAS oncogene family | ![]() |
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2 | 4 | ||||||
MIRT542245 | HSPA4L | heat shock protein family A (Hsp70) member 4 like | ![]() |
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2 | 2 | ||||||
MIRT543299 | ZNF585B | zinc finger protein 585B | ![]() |
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2 | 2 | ||||||
MIRT552456 | ZNF410 | zinc finger protein 410 | ![]() |
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2 | 2 | ||||||
MIRT553641 | TJAP1 | tight junction associated protein 1 | ![]() |
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2 | 2 | ||||||
MIRT557103 | HOXA3 | homeobox A3 | ![]() |
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2 | 2 | ||||||
MIRT570782 | FANCA | Fanconi anemia complementation group A | ![]() |
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2 | 2 | ||||||
MIRT630912 | ZMAT2 | zinc finger matrin-type 2 | ![]() |
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2 | 2 | ||||||
MIRT631034 | ZNF878 | zinc finger protein 878 | ![]() |
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2 | 2 | ||||||
MIRT639017 | AAK1 | AP2 associated kinase 1 | ![]() |
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2 | 2 | ||||||
MIRT648596 | ZYG11B | zyg-11 family member B, cell cycle regulator | ![]() |
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2 | 2 | ||||||
MIRT648939 | ATP5A1 | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle | ![]() |
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2 | 2 | ||||||
MIRT652834 | TACO1 | translational activator of cytochrome c oxidase I | ![]() |
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2 | 2 | ||||||
MIRT659347 | CSRP1 | cysteine and glycine rich protein 1 | ![]() |
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2 | 2 | ||||||
MIRT664163 | APOBEC3F | apolipoprotein B mRNA editing enzyme catalytic subunit 3F | ![]() |
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2 | 2 | ||||||
MIRT670511 | ZSCAN22 | zinc finger and SCAN domain containing 22 | ![]() |
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2 | 2 | ||||||
MIRT671246 | TMEM41B | transmembrane protein 41B | ![]() |
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2 | 2 | ||||||
MIRT672120 | ATP6V0A2 | ATPase H+ transporting V0 subunit a2 | ![]() |
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2 | 2 | ||||||
MIRT672313 | CD3D | CD3d molecule | ![]() |
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2 | 2 | ||||||
MIRT672543 | BRMS1L | breast cancer metastasis-suppressor 1 like | ![]() |
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2 | 2 | ||||||
MIRT673036 | SGPL1 | sphingosine-1-phosphate lyase 1 | ![]() |
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2 | 2 | ||||||
MIRT673814 | DARS | aspartyl-tRNA synthetase | ![]() |
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2 | 2 | ||||||
MIRT674858 | GINM1 | glycoprotein integral membrane 1 | ![]() |
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2 | 2 | ||||||
MIRT674970 | SH3BP2 | SH3 domain binding protein 2 | ![]() |
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2 | 2 | ||||||
MIRT675185 | KIF1C | kinesin family member 1C | ![]() |
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2 | 2 | ||||||
MIRT675219 | UGDH | UDP-glucose 6-dehydrogenase | ![]() |
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2 | 2 | ||||||
MIRT675558 | MED16 | mediator complex subunit 16 | ![]() |
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2 | 2 | ||||||
MIRT682566 | EIF4EBP1 | eukaryotic translation initiation factor 4E binding protein 1 | ![]() |
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2 | 2 | ||||||
MIRT684640 | PDE4C | phosphodiesterase 4C | ![]() |
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2 | 2 | ||||||
MIRT689722 | ATXN2 | ataxin 2 | ![]() |
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2 | 2 | ||||||
MIRT693594 | SLC39A1 | solute carrier family 39 member 1 | ![]() |
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2 | 2 | ||||||
MIRT704745 | CDKN2B | cyclin dependent kinase inhibitor 2B | ![]() |
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2 | 2 | ||||||
MIRT712779 | ZNF154 | zinc finger protein 154 | ![]() |
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2 | 2 | ||||||
MIRT718118 | OTOF | otoferlin | ![]() |
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2 | 2 | ||||||
MIRT720115 | SAMD4A | sterile alpha motif domain containing 4A | ![]() |
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2 | 2 | ||||||
MIRT720275 | EIF1AD | eukaryotic translation initiation factor 1A domain containing | ![]() |
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2 | 2 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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