ID	Duplex structure	Position	Score	MFE
1	miRNA 3' cgaGAAAAGUAA---UUACAUcugu 5' |||| :||| |||| Target 5' cggCTTTGTATTTTCTCTGTAataa 3'	79 - 103	95.00	-6.00
2	miRNA 3' cgaGAAAAGUAAUUACAUCUgu 5' |||||| | :|| :|| Target 5' gtaCTTTTCCCT-GTGAGGAaa 3'	58 - 78	94.00	-7.00
3	miRNA 3' cgaGAAAAGUAAUU---ACAUCUgu 5' || || | | || ||| Target 5' aggCTCATCTGTCACCCTGGAGAag 3'	26 - 50	91.00	-5.90

Mutant ID	Mutant Position	Mutant Source
COSN29507446	14	COSMIC
COSN5718265	45	COSMIC
COSN509510	58	COSMIC
COSN31487097	71	COSMIC
COSN30104032	75	COSMIC
COSN30163260	79	COSMIC
COSN21742469	147	COSMIC
COSN29331271	242	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs202063773	13	dbSNP
rs747076308	14	dbSNP
rs1349508229	16	dbSNP
rs780173339	21	dbSNP
rs1339944454	28	dbSNP
rs1287151872	34	dbSNP
rs1341563155	35	dbSNP
rs558930437	36	dbSNP
rs746422940	38	dbSNP
rs779296628	50	dbSNP
rs1276853964	51	dbSNP
rs1218446019	59	dbSNP
rs545444196	68	dbSNP
rs1017125758	79	dbSNP
rs555793395	80	dbSNP
rs1006105633	83	dbSNP
rs923102481	86	dbSNP
rs1219861442	97	dbSNP
rs112821786	106	dbSNP
rs1173585907	114	dbSNP
rs148689307	118	dbSNP
rs1488465554	135	dbSNP
rs961969381	138	dbSNP
rs1015284943	147	dbSNP
rs1371202189	148	dbSNP
rs3948654	150	dbSNP
rs1200973272	155	dbSNP
rs544027647	161	dbSNP
rs1422938862	169	dbSNP
rs1300234475	170	dbSNP
rs1014292193	175	dbSNP
rs144091628	178	dbSNP
rs895856781	183	dbSNP
rs1316691302	187	dbSNP
rs1471296452	194	dbSNP
rs182821557	195	dbSNP
rs1284254604	196	dbSNP
rs1182046876	197	dbSNP
rs1481193290	199	dbSNP
rs2109429	202	dbSNP
rs1243166297	206	dbSNP
rs2109428	210	dbSNP
rs545515193	217	dbSNP
rs1208365594	219	dbSNP
rs1053518721	221	dbSNP
rs934621121	228	dbSNP
rs1259463853	236	dbSNP
rs1444042956	237	dbSNP
rs1025116956	243	dbSNP
rs1014191738	244	dbSNP
rs1442807639	245	dbSNP
rs892703780	255	dbSNP
rs904413134	256	dbSNP
rs1327117815	257	dbSNP
rs1048402	262	dbSNP
rs1157182877	273	dbSNP
rs1404869294	274	dbSNP
rs1397093851	275	dbSNP
rs943148009	276	dbSNP
rs1334040962	278	dbSNP
rs189909858	290	dbSNP
rs74056976	298	dbSNP
rs1050432180	299	dbSNP
rs539133668	313	dbSNP
rs1239041110	327	dbSNP
rs2159529	335	dbSNP
rs1258705414	342	dbSNP
rs764479094	344	dbSNP
rs1346810479	351	dbSNP
rs868836795	356	dbSNP
rs760957583	364	dbSNP
rs1277759841	366	dbSNP
rs1459930803	368	dbSNP
rs770421378	369	dbSNP
rs1238935024	370	dbSNP
rs1359403560	370	dbSNP
rs1178092490	371	dbSNP
rs1379623476	372	dbSNP
rs923377063	377	dbSNP
rs1172362893	381	dbSNP
rs1404527440	386	dbSNP

- Nature, 2013

Circular RNAs (circRNAs) in animals are an enigmatic class of RNA with unknown function. To explore circRNAs systematically, we sequenced and computationally analysed human, mouse and nematode RNA. We detected thousands of well-expressed, stable circRNAs, often showing tissue/developmental-stage-specific expression. Sequence analysis indicated important regulatory functions for circRNAs. We found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7. Further analyses indicated that CDR1as functions to bind miR-7 in neuronal tissues. Human CDR1as expression in zebrafish impaired midbrain development, similar to knocking down miR-7, suggesting that CDR1as is a miRNA antagonist with a miRNA-binding capacity ten times higher than any other known transcript. Together, our data provide evidence that circRNAs form a large class of post-transcriptional regulators. Numerous circRNAs form by head-to-tail splicing of exons, suggesting previously unrecognized regulatory potential of coding sequences.