ID	Duplex structure	Position	Score	MFE
1	miRNA 3' cgugaGUUUGACA-----CC--CCCGUGAa 5' :||| ||| || ||||||| Target 5' tgtaaTAAA-TGTTTTCAGGCCGGGCACTg 3'	155 - 183	147.00	-14.70
2	miRNA 3' cgUGAGUUUGACACC-CCCGUGAa 5' :|| |: || || ||||| | Target 5' gaGCTGAGCATGGGGTGGGCAGTg 3'	48 - 71	125.00	-13.90
3	miRNA 3' cgugaGUUUG-A-CACC-CCCGUGaa 5' |:::| | |||| ||||:| Target 5' ttagcCGGGCGTGGTGGCGGGCGCct 3'	304 - 329	118.00	-20.70

Mutant ID	Mutant Position	Mutant Source
COSN30459943	20	COSMIC
COSN30109375	38	COSMIC
COSN31588330	101	COSMIC
COSN26885962	175	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs143630867	2	dbSNP
rs1430391587	8	dbSNP
rs1169724549	10	dbSNP
rs1435969330	12	dbSNP
rs1183751799	13	dbSNP
rs189443534	18	dbSNP
rs1315236764	19	dbSNP
rs752729384	19	dbSNP
rs1164567434	22	dbSNP
rs758508571	23	dbSNP
rs545081183	24	dbSNP
rs895915626	26	dbSNP
rs1015745669	27	dbSNP
rs1448240901	30	dbSNP
rs747248238	35	dbSNP
rs148091905	39	dbSNP
rs780913337	40	dbSNP
rs1331307056	42	dbSNP
rs1336655998	43	dbSNP
rs1014730742	46	dbSNP
rs1414545445	51	dbSNP
rs745782671	52	dbSNP
rs1359169313	55	dbSNP
rs1229991539	62	dbSNP
rs1298224656	64	dbSNP
rs762427268	66	dbSNP
rs1231761046	75	dbSNP
rs1027129825	78	dbSNP
rs28924119	88	dbSNP
rs990414701	89	dbSNP
rs1240597055	98	dbSNP
rs548891111	99	dbSNP
rs1466300437	100	dbSNP
rs750907289	101	dbSNP
rs1000360701	102	dbSNP
rs560831126	110	dbSNP
rs949300072	122	dbSNP
rs180671706	128	dbSNP
rs964272176	138	dbSNP
rs1166046950	139	dbSNP
rs1063301	142	dbSNP
rs936470578	148	dbSNP
rs571259638	149	dbSNP
rs28924118	157	dbSNP
rs1370125375	170	dbSNP
rs987588651	176	dbSNP
rs28924117	177	dbSNP
rs1348499089	179	dbSNP
rs1235035540	183	dbSNP
rs565719714	192	dbSNP
rs535852521	193	dbSNP
rs28924116	196	dbSNP
rs973240654	199	dbSNP
rs1252402575	201	dbSNP
rs1467221788	202	dbSNP
rs891157008	205	dbSNP
rs1201560217	211	dbSNP
rs1320883822	220	dbSNP
rs761044183	221	dbSNP
rs1157259943	229	dbSNP
rs1253233161	230	dbSNP
rs1008472187	234	dbSNP
rs1409269182	236	dbSNP
rs574356157	237	dbSNP
rs1204476205	238	dbSNP
rs187419277	239	dbSNP
rs897424483	240	dbSNP
rs1189234467	241	dbSNP
rs1384569018	248	dbSNP
rs1325436494	251	dbSNP
rs1055740796	255	dbSNP
rs879585824	270	dbSNP
rs1422424979	271	dbSNP
rs1229203862	272	dbSNP
rs372961686	281	dbSNP
rs1332694783	296	dbSNP
rs111774484	311	dbSNP
rs1374391772	313	dbSNP
rs1437799557	314	dbSNP
rs558082678	315	dbSNP
rs1254355328	322	dbSNP
rs541273807	323	dbSNP
rs990568020	324	dbSNP
rs1024576916	325	dbSNP
rs970393538	326	dbSNP
rs977795704	327	dbSNP
rs923830466	328	dbSNP
rs556752812	331	dbSNP
rs578102045	334	dbSNP
rs1409252915	345	dbSNP
rs1306142140	346	dbSNP
rs1416612738	347	dbSNP
rs1410223662	357	dbSNP
rs1292891414	362	dbSNP
rs1353872732	364	dbSNP
rs1247042719	371	dbSNP
rs1284903276	375	dbSNP
rs989683885	378	dbSNP
rs545528275	379	dbSNP
rs932705730	381	dbSNP
rs1277398218	385	dbSNP
rs1140272	386	dbSNP
rs1394002124	402	dbSNP
rs891230125	403	dbSNP
rs943996874	405	dbSNP
rs1037040003	407	dbSNP
rs532871466	409	dbSNP
rs572437507	410	dbSNP
rs1166578593	411	dbSNP
rs1049037786	420	dbSNP
rs895340703	430	dbSNP
rs954494281	431	dbSNP
rs1011936619	432	dbSNP
rs550926859	437	dbSNP
rs987148842	439	dbSNP
rs1015109775	440	dbSNP
rs970467151	441	dbSNP
rs1325857906	443	dbSNP
rs999558028	451	dbSNP
rs961806099	457	dbSNP
rs1030673884	470	dbSNP
rs1482497295	472	dbSNP
rs1278575364	480	dbSNP
rs1484462159	480	dbSNP
rs1201753715	483	dbSNP
rs958186852	484	dbSNP
rs1188601051	486	dbSNP
rs1445906028	488	dbSNP
rs1262837647	491	dbSNP
rs1391915153	498	dbSNP
rs1450059573	500	dbSNP

- Nature, 2013

Circular RNAs (circRNAs) in animals are an enigmatic class of RNA with unknown function. To explore circRNAs systematically, we sequenced and computationally analysed human, mouse and nematode RNA. We detected thousands of well-expressed, stable circRNAs, often showing tissue/developmental-stage-specific expression. Sequence analysis indicated important regulatory functions for circRNAs. We found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7. Further analyses indicated that CDR1as functions to bind miR-7 in neuronal tissues. Human CDR1as expression in zebrafish impaired midbrain development, similar to knocking down miR-7, suggesting that CDR1as is a miRNA antagonist with a miRNA-binding capacity ten times higher than any other known transcript. Together, our data provide evidence that circRNAs form a large class of post-transcriptional regulators. Numerous circRNAs form by head-to-tail splicing of exons, suggesting previously unrecognized regulatory potential of coding sequences.