miRTarBase - #MIRT513323


—
pre-miRNA Information
pre-miRNA hsa-mir-6715b   
Genomic Coordinates chr10: 112299612 - 112299688
Description Homo sapiens miR-6715b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-6715b-3p
Sequence 46| CUCAAACCGGCUGUGCCUGUGG |67
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs761643095 7 dbSNP
rs768295556 8 dbSNP
rs182337914 9 dbSNP
rs1369684979 11 dbSNP
rs776704371 13 dbSNP
rs1039819520 15 dbSNP
rs771110697 18 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol KCNMB1   
Synonyms BKbeta1, K(VCA)beta, SLO-BETA, hbeta1, hslo-beta, k(VCA)beta-1, slo-beta-1
Description potassium calcium-activated channel subfamily M regulatory beta subunit 1
Transcript NM_004137   
Expression
Putative miRNA Targets on KCNMB1
3'UTR of KCNMB1
(miRNA target sites are highlighted)		 
>KCNMB1|NM_004137|3'UTR
   1 AGCCATCCATCCATGCCATACCACTTGTCAGGGCACAGGGGACTGGCTGGGCCCCCAGGGCTGCTCCCCACTTGCAGCAC
  81 AATGCCTTCTCCACCTGCCCTCCCACTCTTCCAGTCCAATCCACGCTGTCTTCTGTTGCAGGACTAACCTTTGAGAAATC
 161 CTTTTGTGAAGTCATTGCCTGCTCAAGAATGTACAGTGGCTCCCCAATGCCTTGGAGGCCATAAGGCCAGCCAGTTCTAG
 241 CTCTCTATTACCTGTCCCCACTCAACTGACTCATACCTGTTTCCGGCTGCATCACTATGTGCCCCACAGAGAACGATGAT
 321 CGTCACCTCTGTGCCTGAGTTCTCCCTGTTGTCTCAAAGCGGTACCCATCCTCCCCCAGAAGCTGTCCCCAGCGAGCCTC
 401 CCTTCTTTGTTTGAATTCTCTAATAAGAGCAACAGCAGCTCCCATTTCTAGAACACATTTACAGTATTACTATTTTCTAG
 481 GATATAAAGTGCCATATATATTTTTAATTCCAATATTAATAAATGTATGCCAAACAACAATCAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gguguccgugucggccAAACUc 5'
                          ||||| 
Target 5' tgttgcaggactaaccTTTGAg 3'
 134 - 155 100.00 -12.60
2 
miRNA  3' gguGU-CCGUGUC----GGCCAAACUc 5'
             || |||||||    |:|| | |: 
Target 5' tgtCAGGGCACAGGGGACTGGCTGGGc 3'
 26 - 52 89.00 -20.90
3 
miRNA  3' ggUGUCCGUGU-CGGCCAAAcuc 5'
            || | | ||  || ||||   
Target 5' caACTGACTCATACCTGTTTccg 3'
 263 - 285 88.00 -6.24
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN14155910 6 COSMIC
COSN28434645 7 COSMIC
COSN31487682 49 COSMIC
COSN21399158 51 COSMIC
COSN30469764 54 COSMIC
COSN18189286 60 COSMIC
COSN31480477 67 COSMIC
COSN26981102 78 COSMIC
COSN26981101 79 COSMIC
COSN30162924 98 COSMIC
COSN30494433 101 COSMIC
COSN30453978 104 COSMIC
COSN30181889 122 COSMIC
COSN30523346 153 COSMIC
COSN7911262 186 COSMIC
COSN30535320 207 COSMIC
COSN30162974 215 COSMIC
COSN17182027 224 COSMIC
COSN20102177 378 COSMIC
COSN17407090 418 COSMIC
COSN7911261 459 COSMIC
COSN25665547 471 COSMIC
COSN5072817 664 COSMIC
COSN9933280 675 COSMIC
COSN18408149 738 COSMIC
COSN29212330 1029 COSMIC
COSN20955490 1031 COSMIC
COSN14784374 1053 COSMIC
COSN9933279 1156 COSMIC
COSN15935828 1193 COSMIC
COSN14868866 1340 COSMIC
COSN1311670 1361 COSMIC
COSN20321816 1419 COSMIC
COSN7911260 1529 COSMIC
COSN8208638 1531 COSMIC
COSN26493208 1847 COSMIC
COSN6283188 1919 COSMIC
COSN6510991 1932 COSMIC
COSN16534500 1969 COSMIC
COSN29388955 2026 COSMIC
COSN25011240 2076 COSMIC
COSN26426099 2235 COSMIC
COSN25296778 2243 COSMIC
COSN26520631 2290 COSMIC
COSN20791048 2293 COSMIC
COSN5023481 2387 COSMIC
COSN24237006 2488 COSMIC
COSN25632824 2586 COSMIC
COSN29188285 2667 COSMIC
COSN9419720 2716 COSMIC
COSN8208637 2748 COSMIC
COSN18322722 2936 COSMIC
COSN21104185 3035 COSMIC
COSN23782692 3115 COSMIC
COSN17097089 3117 COSMIC
COSN24230553 3211 COSMIC
COSN23867048 3290 COSMIC
COSN25875606 3380 COSMIC
COSN8208636 3500 COSMIC
COSN17820988 3572 COSMIC
COSN22856851 3775 COSMIC
COSN7911259 3902 COSMIC
COSN5159508 3959 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs961858920 4 dbSNP
rs1412380773 5 dbSNP
rs1376673802 6 dbSNP
rs752271378 8 dbSNP
rs767848017 9 dbSNP
rs979330926 10 dbSNP
rs369090668 16 dbSNP
rs752174051 18 dbSNP
rs766849311 19 dbSNP
rs1417757601 21 dbSNP
rs763450990 22 dbSNP
rs1354070862 24 dbSNP
rs1200462838 25 dbSNP
rs773284305 31 dbSNP
rs765520720 34 dbSNP
rs761760554 36 dbSNP
rs1258407300 37 dbSNP
rs376678526 39 dbSNP
rs112121155 40 dbSNP
rs1269701916 41 dbSNP
rs745791562 42 dbSNP
rs1318610226 43 dbSNP
rs562075054 49 dbSNP
rs770678288 51 dbSNP
rs1013326653 52 dbSNP
rs1290327773 54 dbSNP
rs1205286076 55 dbSNP
rs1371437560 59 dbSNP
rs1006544614 66 dbSNP
rs181390920 68 dbSNP
rs2656842 78 dbSNP
rs2656841 79 dbSNP
rs386694845 80 dbSNP
rs879549256 80 dbSNP
rs999093750 81 dbSNP
rs763261723 82 dbSNP
rs79326375 85 dbSNP
rs1038575025 88 dbSNP
rs1043226550 89 dbSNP
rs1303863527 95 dbSNP
rs767066828 100 dbSNP
rs1387030071 102 dbSNP
rs1385245208 104 dbSNP
rs1011289587 106 dbSNP
rs79790664 107 dbSNP
rs45530435 114 dbSNP
rs142022522 119 dbSNP
rs773989505 124 dbSNP
rs1231203937 125 dbSNP
rs1267017448 128 dbSNP
rs1166146112 130 dbSNP
rs770812987 139 dbSNP
rs922959445 142 dbSNP
rs1036250676 148 dbSNP
rs917219582 149 dbSNP
rs989321122 153 dbSNP
rs1489303742 160 dbSNP
rs1194558280 164 dbSNP
rs1257392181 166 dbSNP
rs1163785180 177 dbSNP
rs940607147 178 dbSNP
rs1167282526 181 dbSNP
rs909006858 190 dbSNP
rs1369418979 192 dbSNP
rs926026793 196 dbSNP
rs574045345 201 dbSNP
rs1377920158 205 dbSNP
rs554593379 207 dbSNP
rs969283102 212 dbSNP
rs1401275604 214 dbSNP
rs915980360 236 dbSNP
rs1267776935 238 dbSNP
rs779455982 243 dbSNP
rs763007438 245 dbSNP
rs1182232577 247 dbSNP
rs991612765 247 dbSNP
rs1483780692 254 dbSNP
rs960301528 256 dbSNP
rs1018154367 257 dbSNP
rs773226024 258 dbSNP
rs1195659496 261 dbSNP
rs1259178379 264 dbSNP
rs1035668575 266 dbSNP
rs1217398653 269 dbSNP
rs1346339486 271 dbSNP
rs985354668 277 dbSNP
rs3209578 279 dbSNP
rs999041513 281 dbSNP
rs12052 283 dbSNP
rs190556888 284 dbSNP
rs1021864702 285 dbSNP
rs1180158781 296 dbSNP
rs314156 297 dbSNP
rs1421162545 298 dbSNP
rs1158681072 301 dbSNP
rs889311754 308 dbSNP
rs896924714 311 dbSNP
rs1457970632 314 dbSNP
rs1370422686 321 dbSNP
rs558443995 322 dbSNP
rs1348780122 326 dbSNP
rs1005759817 341 dbSNP
rs905998753 350 dbSNP
rs1241183743 356 dbSNP
rs997286859 357 dbSNP
rs1409243910 360 dbSNP
rs950308198 361 dbSNP
rs1257070550 363 dbSNP
rs1167271465 364 dbSNP
rs772113831 368 dbSNP
rs1468077097 370 dbSNP
rs1428143295 377 dbSNP
rs567032700 379 dbSNP
rs1473369035 387 dbSNP
rs901485415 389 dbSNP
rs1036031129 393 dbSNP
rs1430943582 394 dbSNP
rs940554988 402 dbSNP
rs895819005 427 dbSNP
rs1443454177 428 dbSNP
rs538807095 433 dbSNP
rs549018198 439 dbSNP
rs1461436535 442 dbSNP
rs1328992513 444 dbSNP
rs909121227 457 dbSNP
rs1258923302 459 dbSNP
rs1402216964 461 dbSNP
rs1447324638 462 dbSNP
rs549019590 463 dbSNP
rs1049013019 473 dbSNP
rs1365809693 477 dbSNP
rs187166302 491 dbSNP
rs1220995797 525 dbSNP
rs916447973 527 dbSNP
rs1307386335 537 dbSNP
rs777940222 548 dbSNP
rs1260330505 552 dbSNP
rs938789538 553 dbSNP
rs1230392996 556 dbSNP
rs1251954094 558 dbSNP
rs1487327729 559 dbSNP
rs1043087539 561 dbSNP
rs1327360530 561 dbSNP
rs768737400 563 dbSNP
rs1168435947 573 dbSNP
rs1425776858 574 dbSNP
rs1465919651 579 dbSNP
rs1448892036 580 dbSNP
rs928602620 582 dbSNP
rs536872325 583 dbSNP
rs314155 585 dbSNP
rs1405173706 591 dbSNP
rs1398291488 594 dbSNP
rs148172346 605 dbSNP
rs758709677 607 dbSNP
rs953642314 628 dbSNP
rs1029179553 629 dbSNP
rs997635858 638 dbSNP
rs1319505792 639 dbSNP
rs901600258 641 dbSNP
rs370013378 642 dbSNP
rs1016702814 649 dbSNP
rs1005267841 662 dbSNP
rs1014716789 664 dbSNP
rs1004549295 666 dbSNP
rs887466729 674 dbSNP
rs182067976 675 dbSNP
rs1163893659 679 dbSNP
rs1411362658 686 dbSNP
rs1370688830 693 dbSNP
rs1357691895 696 dbSNP
rs1165734095 698 dbSNP
rs528563754 701 dbSNP
rs566195333 703 dbSNP
rs1300168298 709 dbSNP
rs1014823722 716 dbSNP
rs1248971149 719 dbSNP
rs750938291 730 dbSNP
rs34613375 731 dbSNP
rs1283878314 736 dbSNP
rs552414385 738 dbSNP
rs755681336 754 dbSNP
rs1287584043 758 dbSNP
rs977498136 767 dbSNP
rs1488539464 770 dbSNP
rs756102284 772 dbSNP
rs914591029 773 dbSNP
rs879313256 775 dbSNP
rs767244007 776 dbSNP
rs1407502264 787 dbSNP
rs57521796 788 dbSNP
rs1243600690 798 dbSNP
rs563541824 799 dbSNP
rs751175710 802 dbSNP
rs1437055922 813 dbSNP
rs1449187014 815 dbSNP
rs1343049775 816 dbSNP
rs976241159 821 dbSNP
rs943426391 825 dbSNP
rs910620649 826 dbSNP
rs766290939 836 dbSNP
rs966240429 837 dbSNP
rs1405440451 840 dbSNP
rs543702333 841 dbSNP
rs1014664445 842 dbSNP
rs1004665473 843 dbSNP
rs1237373284 859 dbSNP
rs1437987340 864 dbSNP
rs1180753882 867 dbSNP
rs530363409 869 dbSNP
rs1381213233 873 dbSNP
rs1049604126 875 dbSNP
rs933814163 876 dbSNP
rs887414393 879 dbSNP
rs1027343034 880 dbSNP
rs1012541083 881 dbSNP
rs973029675 885 dbSNP
rs961037095 893 dbSNP
rs1241734178 895 dbSNP
rs1377232012 897 dbSNP
rs1231859005 899 dbSNP
rs190676990 902 dbSNP
rs1183065500 903 dbSNP
rs1219742189 907 dbSNP
rs1257125138 910 dbSNP
rs1056821317 915 dbSNP
rs1456265491 916 dbSNP
rs1200352831 924 dbSNP
rs1003310292 927 dbSNP
rs1289616956 928 dbSNP
rs969824449 929 dbSNP
rs1379450329 934 dbSNP
rs1023119307 939 dbSNP
rs781208054 941 dbSNP
rs1041754058 942 dbSNP
rs946152807 950 dbSNP
rs1014464858 956 dbSNP
rs1171890288 965 dbSNP
rs184122632 967 dbSNP
rs1359685882 971 dbSNP
rs180927029 974 dbSNP
rs932009554 975 dbSNP
rs111240623 978 dbSNP
rs1351279598 980 dbSNP
rs754890417 991 dbSNP
rs762772187 993 dbSNP
rs966188158 995 dbSNP
rs1023957960 996 dbSNP
rs1371694594 1000 dbSNP
rs558586661 1001 dbSNP
rs751383351 1003 dbSNP
rs1052276039 1006 dbSNP
rs933849009 1007 dbSNP
rs1171784436 1008 dbSNP
rs1368255980 1013 dbSNP
rs545132918 1015 dbSNP
rs1479104709 1024 dbSNP
rs1267083805 1027 dbSNP
rs1027702050 1028 dbSNP
rs576259601 1030 dbSNP
rs556444904 1031 dbSNP
rs1275256735 1036 dbSNP
rs773351111 1038 dbSNP
rs1339639359 1039 dbSNP
rs765251628 1039 dbSNP
rs909788474 1045 dbSNP
rs5013454 1053 dbSNP
rs1003426479 1055 dbSNP
rs902331230 1057 dbSNP
rs529256278 1062 dbSNP
rs77069032 1067 dbSNP
rs188285369 1068 dbSNP
rs1403788545 1069 dbSNP
rs1054986687 1078 dbSNP
rs5873226 1082 dbSNP
rs931984462 1089 dbSNP
rs921956479 1092 dbSNP
rs1309325034 1093 dbSNP
rs1440578409 1098 dbSNP
rs1444900226 1106 dbSNP
rs1157464301 1107 dbSNP
rs1040621929 1108 dbSNP
rs1354495837 1112 dbSNP
rs1289371769 1121 dbSNP
rs5873225 1122 dbSNP
rs1314638580 1123 dbSNP
rs1394047060 1123 dbSNP
rs369385575 1123 dbSNP
rs543860953 1123 dbSNP
rs1355928783 1129 dbSNP
rs1217521546 1130 dbSNP
rs554647080 1130 dbSNP
rs534990835 1132 dbSNP
rs1202506530 1137 dbSNP
rs1257310968 1137 dbSNP
rs1427443042 1137 dbSNP
rs565958357 1144 dbSNP
rs968963175 1145 dbSNP
rs983629916 1150 dbSNP
rs1378171407 1151 dbSNP
rs1021828377 1161 dbSNP
rs952348918 1169 dbSNP
rs1159261277 1170 dbSNP
rs920337236 1173 dbSNP
rs991003119 1179 dbSNP
rs201138501 1187 dbSNP
rs1406560529 1189 dbSNP
rs889082999 1191 dbSNP
rs959039944 1194 dbSNP
rs1329551350 1195 dbSNP
rs1030420221 1199 dbSNP
rs1224384067 1203 dbSNP
rs1300718900 1205 dbSNP
rs997987561 1212 dbSNP
rs1236090077 1215 dbSNP
rs1157800697 1217 dbSNP
rs1034665625 1221 dbSNP
rs1197189433 1224 dbSNP
rs750342138 1229 dbSNP
rs1175578740 1232 dbSNP
rs1235956869 1233 dbSNP
rs966605447 1235 dbSNP
rs552204333 1236 dbSNP
rs532514221 1245 dbSNP
rs888405263 1249 dbSNP
rs1048335299 1252 dbSNP
rs569904007 1253 dbSNP
rs1325494870 1254 dbSNP
rs1464878024 1254 dbSNP
rs915537285 1266 dbSNP
rs143425850 1276 dbSNP
rs549994793 1277 dbSNP
rs1292959665 1280 dbSNP
rs1010737234 1295 dbSNP
rs1388666349 1298 dbSNP
rs530306421 1301 dbSNP
rs924363550 1305 dbSNP
rs1217133790 1306 dbSNP
rs1260426930 1312 dbSNP
rs747214433 1313 dbSNP
rs977596545 1317 dbSNP
rs1264551820 1327 dbSNP
rs968848645 1329 dbSNP
rs1192466218 1330 dbSNP
rs4309989 1340 dbSNP
rs1477466311 1344 dbSNP
rs1191159085 1348 dbSNP
rs183899897 1352 dbSNP
rs900478480 1357 dbSNP
rs1040402771 1359 dbSNP
rs944908984 1361 dbSNP
rs1348399907 1362 dbSNP
rs1459489252 1366 dbSNP
rs370163005 1377 dbSNP
rs1303989874 1380 dbSNP
rs527242955 1382 dbSNP
rs72834387 1388 dbSNP
rs997514194 1388 dbSNP
rs1454911028 1391 dbSNP
rs1363076249 1400 dbSNP
rs112585144 1405 dbSNP
rs545122430 1411 dbSNP
rs746224597 1412 dbSNP
rs1004083716 1414 dbSNP
rs1457921429 1420 dbSNP
rs1487890472 1427 dbSNP
rs888241289 1428 dbSNP
rs1156975113 1432 dbSNP
rs77794919 1433 dbSNP
rs920772294 1434 dbSNP
rs990560254 1435 dbSNP
rs1182445142 1445 dbSNP
rs1164216692 1457 dbSNP
rs1359993747 1465 dbSNP
rs1462604446 1470 dbSNP
rs1240981541 1471 dbSNP
rs1300581306 1480 dbSNP
rs779447770 1488 dbSNP
rs753079449 1489 dbSNP
rs1282810475 1492 dbSNP
rs927598451 1493 dbSNP
rs1247739854 1494 dbSNP
rs938355763 1498 dbSNP
rs113923255 1504 dbSNP
rs1286234295 1505 dbSNP
rs966387836 1520 dbSNP
rs1315880130 1521 dbSNP
rs1041412687 1528 dbSNP
rs137959944 1529 dbSNP
rs989832160 1530 dbSNP
rs749766707 1531 dbSNP
rs1414428231 1533 dbSNP
rs542873989 1534 dbSNP
rs957868008 1540 dbSNP
rs1453921696 1546 dbSNP
rs780594265 1547 dbSNP
rs1028594262 1548 dbSNP
rs573778628 1550 dbSNP
rs1436330963 1553 dbSNP
rs1387387387 1554 dbSNP
rs1280815838 1556 dbSNP
rs754452408 1561 dbSNP
rs900597670 1562 dbSNP
rs1350477568 1566 dbSNP
rs1165568314 1569 dbSNP
rs976529099 1571 dbSNP
rs1325418321 1572 dbSNP
rs962064167 1576 dbSNP
rs76157886 1583 dbSNP
rs1184030991 1584 dbSNP
rs1233527188 1586 dbSNP
rs1383347016 1595 dbSNP
rs1477774700 1600 dbSNP
rs1182724331 1613 dbSNP
rs370248313 1616 dbSNP
rs1006286340 1630 dbSNP
rs952536322 1632 dbSNP
rs1399047041 1635 dbSNP
rs533856001 1638 dbSNP
rs1444133263 1640 dbSNP
rs1008902427 1644 dbSNP
rs1012734311 1646 dbSNP
rs886465822 1656 dbSNP
rs766126470 1659 dbSNP
rs1347369839 1667 dbSNP
rs1485347411 1668 dbSNP
rs566060042 1669 dbSNP
rs758328529 1673 dbSNP
rs554002353 1674 dbSNP
rs1197787482 1682 dbSNP
rs1274065425 1683 dbSNP
rs1196910736 1688 dbSNP
rs899290903 1691 dbSNP
rs750219436 1703 dbSNP
rs1055708129 1706 dbSNP
rs1481070539 1729 dbSNP
rs1193318165 1747 dbSNP
rs558566195 1752 dbSNP
rs938226406 1762 dbSNP
rs765304663 1764 dbSNP
rs1421954169 1765 dbSNP
rs761687110 1769 dbSNP
rs981850280 1771 dbSNP
rs776578174 1776 dbSNP
rs914312813 1780 dbSNP
rs1300770557 1782 dbSNP
rs1347278295 1786 dbSNP
rs1405487046 1788 dbSNP
rs913584144 1790 dbSNP
rs75740160 1791 dbSNP
rs1270967853 1794 dbSNP
rs11134627 1795 dbSNP
rs1208099759 1799 dbSNP
rs976328747 1799 dbSNP
rs1490640607 1800 dbSNP
rs1368540903 1803 dbSNP
rs1261737435 1807 dbSNP
rs958199736 1808 dbSNP
rs538729465 1816 dbSNP
rs1472279161 1817 dbSNP
rs1424809040 1818 dbSNP
rs764091182 1818 dbSNP
rs1162780935 1824 dbSNP
rs1382764472 1830 dbSNP
rs952131590 1835 dbSNP
rs975645908 1837 dbSNP
rs569840737 1854 dbSNP
rs759944419 1855 dbSNP
rs76210756 1861 dbSNP
rs1036018222 1865 dbSNP
rs1002771182 1873 dbSNP
rs1360248500 1880 dbSNP
rs950659064 1898 dbSNP
rs536819245 1903 dbSNP
rs146968769 1906 dbSNP
rs1206047571 1915 dbSNP
rs995071858 1917 dbSNP
rs1019939926 1919 dbSNP
rs899400774 1923 dbSNP
rs774667905 1927 dbSNP
rs771244934 1934 dbSNP
rs760738934 1941 dbSNP
rs1291695197 1947 dbSNP
rs1386750945 1959 dbSNP
rs1206582488 1961 dbSNP
rs191865589 1962 dbSNP
rs141319778 1963 dbSNP
rs114197387 1968 dbSNP
rs912796074 1968 dbSNP
rs1046097603 1969 dbSNP
rs1401223243 1972 dbSNP
rs1041037616 1973 dbSNP
rs1338907854 1980 dbSNP
rs1383860284 1983 dbSNP
rs1446060999 1988 dbSNP
rs551432596 1989 dbSNP
rs1403189655 1997 dbSNP
rs1319183918 2001 dbSNP
rs1240405063 2011 dbSNP
rs1345039731 2022 dbSNP
rs913545060 2034 dbSNP
rs1244548139 2046 dbSNP
rs1461515832 2058 dbSNP
rs1177401863 2063 dbSNP
rs1247825245 2065 dbSNP
rs1391946621 2067 dbSNP
rs1398507012 2077 dbSNP
rs1165972748 2083 dbSNP
rs1377039310 2090 dbSNP
rs1468014146 2095 dbSNP
rs1178345265 2098 dbSNP
rs1407057079 2099 dbSNP
rs908106260 2100 dbSNP
rs1296466885 2109 dbSNP
rs984828288 2113 dbSNP
rs746275458 2115 dbSNP
rs1053873756 2121 dbSNP
rs1461252803 2122 dbSNP
rs1224368727 2131 dbSNP
rs774733205 2135 dbSNP
rs771282459 2136 dbSNP
rs1301161972 2140 dbSNP
rs921159691 2143 dbSNP
rs975393286 2144 dbSNP
rs965176574 2148 dbSNP
rs1323733225 2160 dbSNP
rs749772651 2165 dbSNP
rs1035501533 2166 dbSNP
rs1481312006 2170 dbSNP
rs1177321587 2172 dbSNP
rs531413083 2179 dbSNP
rs369805184 2180 dbSNP
rs967293567 2181 dbSNP
rs562543087 2185 dbSNP
rs1181872674 2187 dbSNP
rs1429752113 2192 dbSNP
rs950792912 2195 dbSNP
rs1168593419 2201 dbSNP
rs1291463622 2208 dbSNP
rs1026428980 2219 dbSNP
rs995228117 2221 dbSNP
rs1407313883 2223 dbSNP
rs892770668 2224 dbSNP
rs1248979319 2229 dbSNP
rs1029163526 2230 dbSNP
rs1213223873 2231 dbSNP
rs963451344 2232 dbSNP
rs1292966494 2233 dbSNP
rs1034340038 2241 dbSNP
rs1204555191 2242 dbSNP
rs1002418214 2243 dbSNP
rs1290399737 2243 dbSNP
rs901576225 2244 dbSNP
rs906670850 2245 dbSNP
rs1421869201 2246 dbSNP
rs1046565735 2248 dbSNP
rs1009241495 2249 dbSNP
rs188336004 2250 dbSNP
rs1426703986 2251 dbSNP
rs1221979783 2257 dbSNP
rs1360504965 2259 dbSNP
rs892195826 2261 dbSNP
rs1428998023 2262 dbSNP
rs1053562615 2264 dbSNP
rs573742192 2271 dbSNP
rs920971750 2288 dbSNP
rs1039605098 2289 dbSNP
rs930631369 2290 dbSNP
rs916560457 2292 dbSNP
rs185312972 2293 dbSNP
rs939481636 2294 dbSNP
rs555186650 2296 dbSNP
rs912373345 2303 dbSNP
rs540508327 2305 dbSNP
rs578013820 2306 dbSNP
rs928022549 2313 dbSNP
rs1380179851 2316 dbSNP
rs1467950256 2319 dbSNP
rs577892775 2320 dbSNP
rs1346143627 2321 dbSNP
rs1447846819 2335 dbSNP
rs951217984 2339 dbSNP
rs112854794 2340 dbSNP
rs1315147964 2341 dbSNP
rs191126 2342 dbSNP
rs1349795277 2343 dbSNP
rs973547889 2345 dbSNP
rs1286344535 2349 dbSNP
rs967177317 2361 dbSNP
rs963674099 2365 dbSNP
rs1241473725 2367 dbSNP
rs1362577077 2369 dbSNP
rs913159922 2373 dbSNP
rs1232382661 2375 dbSNP
rs989986742 2377 dbSNP
rs538619820 2382 dbSNP
rs980964657 2383 dbSNP
rs576042588 2386 dbSNP
rs556197589 2387 dbSNP
rs1462147885 2397 dbSNP
rs1028685468 2398 dbSNP
rs1025207416 2399 dbSNP
rs1009671612 2408 dbSNP
rs892672714 2414 dbSNP
rs1032496164 2415 dbSNP
rs1334658748 2420 dbSNP
rs1439447769 2422 dbSNP
rs1000625256 2427 dbSNP
rs899474638 2434 dbSNP
rs1373999849 2439 dbSNP
rs1268948996 2442 dbSNP
rs1261025451 2445 dbSNP
rs996225889 2446 dbSNP
rs779900377 2450 dbSNP
rs371279788 2451 dbSNP
rs891030828 2452 dbSNP
rs1047053384 2453 dbSNP
rs1233916143 2454 dbSNP
rs1480399655 2457 dbSNP
rs1279027747 2458 dbSNP
rs1238312255 2461 dbSNP
rs1383407266 2462 dbSNP
rs567846460 2474 dbSNP
rs1407668802 2475 dbSNP
rs919778440 2480 dbSNP
rs1354967040 2481 dbSNP
rs569781466 2482 dbSNP
rs942144421 2483 dbSNP
rs377677499 2487 dbSNP
rs1269803231 2488 dbSNP
rs1019101855 2492 dbSNP
rs1197588421 2494 dbSNP
rs557930359 2505 dbSNP
rs1490223819 2506 dbSNP
rs1220903257 2508 dbSNP
rs1402084750 2509 dbSNP
rs1490610102 2513 dbSNP
rs34621617 2519 dbSNP
rs1192950364 2520 dbSNP
rs1005059903 2522 dbSNP
rs1382219651 2523 dbSNP
rs1391874135 2525 dbSNP
rs1169080414 2527 dbSNP
rs1390238030 2529 dbSNP
rs1423522601 2530 dbSNP
rs886565633 2532 dbSNP
rs1445810771 2539 dbSNP
rs1387589564 2546 dbSNP
rs970725092 2552 dbSNP
rs71605761 2553 dbSNP
rs1157985074 2554 dbSNP
rs1194870755 2554 dbSNP
rs1204855140 2554 dbSNP
rs1229869380 2554 dbSNP
rs1243939802 2554 dbSNP
rs1271061217 2554 dbSNP
rs1293381122 2554 dbSNP
rs1294528484 2554 dbSNP
rs1324851300 2554 dbSNP
rs1358782215 2554 dbSNP
rs1360837239 2554 dbSNP
rs1405278543 2554 dbSNP
rs1406661432 2554 dbSNP
rs1449151172 2554 dbSNP
rs397885011 2554 dbSNP
rs767409397 2554 dbSNP
rs796684049 2554 dbSNP
rs1025176266 2555 dbSNP
rs13165837 2556 dbSNP
rs1444330775 2556 dbSNP
rs1383749227 2557 dbSNP
rs995060904 2560 dbSNP
rs1242920017 2563 dbSNP
rs895375599 2565 dbSNP
rs1317265762 2570 dbSNP
rs1360727350 2583 dbSNP
rs1238939499 2587 dbSNP
rs1456574282 2597 dbSNP
rs1259550319 2599 dbSNP
rs1445480749 2600 dbSNP
rs1283709522 2612 dbSNP
rs957055145 2613 dbSNP
rs80245638 2615 dbSNP
rs534493028 2619 dbSNP
rs1000997185 2622 dbSNP
rs1181141022 2629 dbSNP
rs899570097 2631 dbSNP
rs1244432331 2637 dbSNP
rs1293788150 2659 dbSNP
rs1180963448 2667 dbSNP
rs1243510113 2667 dbSNP
rs1455111675 2669 dbSNP
rs1381855903 2675 dbSNP
rs554864182 2676 dbSNP
rs928104896 2676 dbSNP
rs1018061330 2691 dbSNP
rs1468446788 2696 dbSNP
rs1007896184 2698 dbSNP
rs1379974334 2700 dbSNP
rs1432524645 2701 dbSNP
rs945886657 2704 dbSNP
rs1338070126 2705 dbSNP
rs750055988 2710 dbSNP
rs1283046772 2717 dbSNP
rs1322323112 2721 dbSNP
rs1317060862 2727 dbSNP
rs989927606 2728 dbSNP
rs1483279158 2737 dbSNP
rs1194758556 2742 dbSNP
rs1424690907 2754 dbSNP
rs1046981934 2756 dbSNP
rs1368017285 2757 dbSNP
rs193002438 2761 dbSNP
rs1420813716 2763 dbSNP
rs921840737 2767 dbSNP
rs898298947 2769 dbSNP
rs1038218013 2782 dbSNP
rs974469089 2796 dbSNP
rs937199056 2808 dbSNP
rs868632954 2809 dbSNP
rs926689839 2815 dbSNP
rs531750296 2816 dbSNP
rs1474570822 2817 dbSNP
rs552127644 2825 dbSNP
rs1399178977 2830 dbSNP
rs1410985809 2832 dbSNP
rs1283285825 2833 dbSNP
rs796067203 2835 dbSNP
rs1225789302 2836 dbSNP
rs314154 2836 dbSNP
rs1312445529 2841 dbSNP
rs314153 2846 dbSNP
rs1481364235 2847 dbSNP
rs866597279 2848 dbSNP
rs1266863142 2851 dbSNP
rs1220246699 2854 dbSNP
rs367667326 2859 dbSNP
rs1489787148 2860 dbSNP
rs1321029906 2861 dbSNP
rs988776964 2865 dbSNP
rs1201342105 2870 dbSNP
rs1273500830 2878 dbSNP
rs549018402 2882 dbSNP
rs1216068850 2884 dbSNP
rs1374851599 2886 dbSNP
rs1430529870 2890 dbSNP
rs956992538 2899 dbSNP
rs529017293 2909 dbSNP
rs797011598 2911 dbSNP
rs189136606 2912 dbSNP
rs1294497156 2913 dbSNP
rs1367802998 2919 dbSNP
rs539786639 2921 dbSNP
rs895440929 2921 dbSNP
rs1055779682 2927 dbSNP
rs1405034768 2931 dbSNP
rs1228558261 2932 dbSNP
rs1290881844 2938 dbSNP
rs1357230803 2941 dbSNP
rs979608135 2948 dbSNP
rs73800639 2952 dbSNP
rs1018029950 2953 dbSNP
rs906488477 2957 dbSNP
rs1042470578 2961 dbSNP
rs1007989476 2971 dbSNP
rs1184969483 2979 dbSNP
rs547685021 2987 dbSNP
rs753719565 2989 dbSNP
rs945435230 2990 dbSNP
rs1355591522 2991 dbSNP
rs577435585 2993 dbSNP
rs915306877 3012 dbSNP
rs1408521875 3021 dbSNP
rs1166187070 3026 dbSNP
rs112361232 3035 dbSNP
rs1419084722 3040 dbSNP
rs1177513069 3046 dbSNP
rs1481536440 3048 dbSNP
rs1054256239 3050 dbSNP
rs954985765 3072 dbSNP
rs764084294 3075 dbSNP
rs760721644 3082 dbSNP
rs1435138489 3083 dbSNP
rs314152 3090 dbSNP
rs1380415799 3093 dbSNP
rs1229946756 3096 dbSNP
rs994462164 3102 dbSNP
rs1290589364 3117 dbSNP
rs544107889 3117 dbSNP
rs1038715579 3118 dbSNP
rs199881224 3118 dbSNP
rs897264259 3120 dbSNP
rs575982207 3124 dbSNP
rs1233339713 3137 dbSNP
rs879813570 3140 dbSNP
rs905736816 3140 dbSNP
rs921880570 3141 dbSNP
rs182136909 3144 dbSNP
rs139001847 3145 dbSNP
rs1399268126 3145 dbSNP
rs879715343 3145 dbSNP
rs949472217 3152 dbSNP
rs1390301248 3153 dbSNP
rs536741654 3166 dbSNP
rs1457453346 3171 dbSNP
rs115267393 3174 dbSNP
rs767547700 3180 dbSNP
rs1449644529 3186 dbSNP
rs935354517 3186 dbSNP
rs1284660666 3187 dbSNP
rs925557584 3190 dbSNP
rs980126514 3195 dbSNP
rs964173521 3200 dbSNP
rs911397119 3209 dbSNP
rs975024167 3211 dbSNP
rs568424888 3212 dbSNP
rs944404593 3213 dbSNP
rs1203270627 3222 dbSNP
rs955122028 3225 dbSNP
rs1261548699 3228 dbSNP
rs911529798 3230 dbSNP
rs1210936641 3238 dbSNP
rs1232766646 3241 dbSNP
rs1469054208 3244 dbSNP
rs1025843555 3245 dbSNP
rs950473010 3258 dbSNP
rs1413143363 3262 dbSNP
rs1480352228 3264 dbSNP
rs1280854953 3273 dbSNP
rs1027404967 3277 dbSNP
rs1374719683 3283 dbSNP
rs376573898 3286 dbSNP
rs973633235 3290 dbSNP
rs1440470722 3293 dbSNP
rs573019654 3296 dbSNP
rs1353799402 3302 dbSNP
rs1354382467 3308 dbSNP
rs1337781646 3314 dbSNP
rs759780667 3315 dbSNP
rs1439922701 3318 dbSNP
rs1471102924 3320 dbSNP
rs114195533 3321 dbSNP
rs962729739 3324 dbSNP
rs1281564194 3327 dbSNP
rs1016842987 3331 dbSNP
rs1408635799 3334 dbSNP
rs1001863910 3341 dbSNP
rs571887590 3346 dbSNP
rs905664096 3347 dbSNP
rs1468523104 3348 dbSNP
rs550034192 3351 dbSNP
rs1237393839 3358 dbSNP
rs558452866 3363 dbSNP
rs1253220355 3366 dbSNP
rs1020934192 3368 dbSNP
rs1014073149 3369 dbSNP
rs1490274785 3374 dbSNP
rs896557982 3395 dbSNP
rs538544814 3397 dbSNP
rs1260808553 3404 dbSNP
rs1052990844 3410 dbSNP
rs1208831529 3412 dbSNP
rs935339031 3421 dbSNP
rs1009519523 3424 dbSNP
rs1172857990 3428 dbSNP
rs893924991 3429 dbSNP
rs796352266 3432 dbSNP
rs1371116398 3445 dbSNP
rs935851728 3446 dbSNP
rs1301074224 3447 dbSNP
rs568858093 3448 dbSNP
rs1043944332 3452 dbSNP
rs189792863 3458 dbSNP
rs942951421 3461 dbSNP
rs1376773335 3462 dbSNP
rs1337497207 3464 dbSNP
rs1230766764 3469 dbSNP
rs771204408 3473 dbSNP
rs1359355243 3474 dbSNP
rs1220645458 3483 dbSNP
rs1269894917 3485 dbSNP
rs911364388 3498 dbSNP
rs867241805 3499 dbSNP
rs73800638 3500 dbSNP
rs1241204622 3512 dbSNP
rs944648885 3516 dbSNP
rs1189979743 3518 dbSNP
rs151012747 3523 dbSNP
rs1423951322 3524 dbSNP
rs1347097354 3525 dbSNP
rs918339284 3529 dbSNP
rs773651895 3541 dbSNP
rs962371216 3544 dbSNP
rs1017011706 3545 dbSNP
rs920351041 3548 dbSNP
rs1298439775 3550 dbSNP
rs973525417 3555 dbSNP
rs1244416199 3556 dbSNP
rs546690112 3569 dbSNP
rs979963912 3590 dbSNP
rs969938076 3591 dbSNP
rs1024202305 3593 dbSNP
rs1189875672 3613 dbSNP
rs1034236993 3618 dbSNP
rs75284231 3622 dbSNP
rs891666348 3629 dbSNP
rs1240884365 3634 dbSNP
rs1180988601 3637 dbSNP
rs1031051202 3647 dbSNP
rs563942521 3652 dbSNP
rs1157787094 3668 dbSNP
rs1485980361 3669 dbSNP
rs1262023025 3682 dbSNP
rs1386654369 3690 dbSNP
rs1212989628 3695 dbSNP
rs544097790 3707 dbSNP
rs1321544902 3713 dbSNP
rs780021279 3714 dbSNP
rs1293068027 3717 dbSNP
rs1009529382 3720 dbSNP
rs1394411239 3721 dbSNP
rs113266060 3731 dbSNP
rs893766494 3734 dbSNP
rs903829541 3735 dbSNP
rs1238704455 3736 dbSNP
rs1278163059 3738 dbSNP
rs1331734607 3739 dbSNP
rs1210207753 3748 dbSNP
rs1032446362 3751 dbSNP
rs1381128814 3760 dbSNP
rs1460468434 3762 dbSNP
rs997309521 3769 dbSNP
rs899899963 3775 dbSNP
rs1383036800 3778 dbSNP
rs1442946486 3781 dbSNP
rs1181067455 3785 dbSNP
rs1473472066 3789 dbSNP
rs1160148744 3792 dbSNP
rs1044129135 3800 dbSNP
rs942706611 3806 dbSNP
rs1304668969 3815 dbSNP
rs1403466480 3818 dbSNP
rs314151 3823 dbSNP
rs1051385511 3828 dbSNP
rs890412628 3831 dbSNP
rs549757731 3833 dbSNP
rs1352682868 3836 dbSNP
rs1229451686 3838 dbSNP
rs1288535993 3839 dbSNP
rs1311283982 3839 dbSNP
rs779741032 3843 dbSNP
rs934137424 3845 dbSNP
rs918756887 3846 dbSNP
rs1483328931 3848 dbSNP
rs1195123976 3849 dbSNP
rs1426465917 3855 dbSNP
rs1483884464 3860 dbSNP
rs1182664785 3861 dbSNP
rs202241516 3867 dbSNP
rs1431676817 3868 dbSNP
rs972467931 3871 dbSNP
rs771658928 3893 dbSNP
rs745525439 3902 dbSNP
rs542685480 3903 dbSNP
rs979722093 3904 dbSNP
rs1372972000 3917 dbSNP
rs1410779949 3920 dbSNP
rs1263348357 3927 dbSNP
rs969736875 3937 dbSNP
rs920236286 3939 dbSNP
rs1024169725 3943 dbSNP
rs1227054866 3950 dbSNP
rs1272332286 3954 dbSNP
rs778557579 3959 dbSNP
rs1225441642 3960 dbSNP
rs1357896097 3962 dbSNP
rs545996130 3966 dbSNP
rs1487422836 3970 dbSNP
rs1193106899 3979 dbSNP
rs757064297 3986 dbSNP
rs372985305 3988 dbSNP
rs574001252 3999 dbSNP
rs1031940885 4010 dbSNP
rs970833275 4020 dbSNP
rs146812894 4027 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in GSM545213. RNA binding protein: AGO2. Condition:Control PAR-CLIP data was present in GSM545215. RNA binding protein: AGO4. Condition:Control ...

- Hafner M; Landthaler M; Burger L; Khorshid et al., 2010, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' gguguccgugucggCCAAACUc 5'
                        ||||||| 
Target 5' ---------ucuugGGUUUGAa 3'
 		1 - 13
Article - Hafner M; Landthaler M; Burger L; Khorshid et al.
- Cell, 2010
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs. The crosslinked sites are revealed by thymidine to cytidine transitions in the cDNAs prepared from immunopurified RNPs of 4-thiouridine-treated cells. We determined the binding sites and regulatory consequences for several intensely studied RBPs and miRNPs, including PUM2, QKI, IGF2BP1-3, AGO/EIF2C1-4 and TNRC6A-C. Our study revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions. The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases.
LinkOut: [PMID: 20371350]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions hESCs (WA-09)
Disease 3779.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in SRR359787. RNA binding protein: AGO2. Condition:4-thiouridine ...

- Lipchina I; Elkabetz Y; Hafner M; Sheridan et al., 2011, Genes & development.

Article - Lipchina I; Elkabetz Y; Hafner M; Sheridan et al.
- Genes & development, 2011
MicroRNAs are important regulators in many cellular processes, including stem cell self-renewal. Recent studies demonstrated their function as pluripotency factors with the capacity for somatic cell reprogramming. However, their role in human embryonic stem (ES) cells (hESCs) remains poorly understood, partially due to the lack of genome-wide strategies to identify their targets. Here, we performed comprehensive microRNA profiling in hESCs and in purified neural and mesenchymal derivatives. Using a combination of AGO cross-linking and microRNA perturbation experiments, together with computational prediction, we identified the targets of the miR-302/367 cluster, the most abundant microRNAs in hESCs. Functional studies identified novel roles of miR-302/367 in maintaining pluripotency and regulating hESC differentiation. We show that in addition to its role in TGF-beta signaling, miR-302/367 promotes bone morphogenetic protein (BMP) signaling by targeting BMP inhibitors TOB2, DAZAP2, and SLAIN1. This study broadens our understanding of microRNA function in hESCs and is a valuable resource for future studies in this area.
LinkOut: [PMID: 22012620]
Experimental Support 3 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HEK293
Disease 3779.0
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "PAR-CLIP data was present in GSM1065668. RNA binding protein: AGO1. Condition:4-thiouridine ...

- Memczak S; Jens M; Elefsinioti A; Torti F; et al., 2013, Nature.

Article - Memczak S; Jens M; Elefsinioti A; Torti F; et al.
- Nature, 2013
Circular RNAs (circRNAs) in animals are an enigmatic class of RNA with unknown function. To explore circRNAs systematically, we sequenced and computationally analysed human, mouse and nematode RNA. We detected thousands of well-expressed, stable circRNAs, often showing tissue/developmental-stage-specific expression. Sequence analysis indicated important regulatory functions for circRNAs. We found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7. Further analyses indicated that CDR1as functions to bind miR-7 in neuronal tissues. Human CDR1as expression in zebrafish impaired midbrain development, similar to knocking down miR-7, suggesting that CDR1as is a miRNA antagonist with a miRNA-binding capacity ten times higher than any other known transcript. Together, our data provide evidence that circRNAs form a large class of post-transcriptional regulators. Numerous circRNAs form by head-to-tail splicing of exons, suggesting previously unrecognized regulatory potential of coding sequences.
LinkOut: [PMID: 23446348]
Experimental Support 4 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions HCT116
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in ERX177603. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_2_5 PAR-CLIP data was present in ERX177618. RNA binding protein: AGO2. Condition:KO_V_AGO_CLIP_3_8 PAR-CLIP data was present in ERX177632. RNA binding protein: AGO2. Condition:p53_V_AGO_CLIP_4_10 PAR-CLIP data was present in ERX177606. RNA binding protein: AGO2. Condition:KO_V_AGO_CLIP_2_8 PAR-CLIP data was present in ERX177623. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_4_1 PAR-CLIP data was present in ERX177630. RNA binding protein: AGO2. Condition:KO_V_AGO_CLIP_4_8 PAR-CLIP data was present in ERX177599. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_2_1 PAR-CLIP data was present in ERX177611. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_3_1 PAR-CLIP data was present in ERX177615. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_3_5 PAR-CLIP data was present in ERX177605. RNA binding protein: AGO2. Condition:KO_D_AGO_CLIP_2_7 PAR-CLIP data was present in ERX177629. RNA binding protein: AGO2. Condition:KO_D_AGO_CLIP_4_7 PAR-CLIP data was present in ERX177627. RNA binding protein: AGO2. Condition:p53_D_AGO_CLIP_4_5 ...

- Krell J; Stebbing J; Carissimi C; Dabrowska et al., 2016, Genome research.

Article - Krell J; Stebbing J; Carissimi C; Dabrowska et al.
- Genome research, 2016
DNA damage activates TP53-regulated surveillance mechanisms that are crucial in suppressing tumorigenesis. TP53 orchestrates these responses directly by transcriptionally modulating genes, including microRNAs (miRNAs), and by regulating miRNA biogenesis through interacting with the DROSHA complex. However, whether the association between miRNAs and AGO2 is regulated following DNA damage is not yet known. Here, we show that, following DNA damage, TP53 interacts with AGO2 to induce or reduce AGO2's association of a subset of miRNAs, including multiple let-7 family members. Furthermore, we show that specific mutations in TP53 decrease rather than increase the association of let-7 family miRNAs, reducing their activity without preventing TP53 from interacting with AGO2. This is consistent with the oncogenic properties of these mutants. Using AGO2 RIP-seq and PAR-CLIP-seq, we show that the DNA damage-induced increase in binding of let-7 family members to the RISC complex is functional. We unambiguously determine the global miRNA-mRNA interaction networks involved in the DNA damage response, validating them through the identification of miRNA-target chimeras formed by endogenous ligation reactions. We find that the target complementary region of the let-7 seed tends to have highly fixed positions and more variable ones. Additionally, we observe that miRNAs, whose cellular abundance or differential association with AGO2 is regulated by TP53, are involved in an intricate network of regulatory feedback and feedforward circuits. TP53-mediated regulation of AGO2-miRNA interaction represents a new mechanism of miRNA regulation in carcinogenesis.
LinkOut: [PMID: 26701625]
Experimental Support 5 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions Prostate Tissue
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in SRX1760641. RNA binding protein: AGO2. Condition:AGO-CLIP-LNCaP-MDV_B PAR-CLIP data was present in SRX1760632. RNA binding protein: AGO2. Condition:AGO-CLIP-22RV1_C PAR-CLIP data was present in SRX1760583. RNA binding protein: AGO2. Condition:AGO-CLIP-LNCaP_A PAR-CLIP data was present in SRX1760639. RNA binding protein: AGO2. Condition:AGO-CLIP-LNCaP-MDV_A ...

- Hamilton MP; Rajapakshe KI; Bader DA; Cerne et al., 2016, Neoplasia (New York, N.Y.).

Article - Hamilton MP; Rajapakshe KI; Bader DA; Cerne et al.
- Neoplasia (New York, N.Y.), 2016
MicroRNA (miRNA) deregulation in prostate cancer (PCa) contributes to PCa initiation and metastatic progression. To comprehensively define the cancer-associated changes in miRNA targeting and function in commonly studied models of PCa, we performed photoactivatable ribonucleoside-enhanced cross-linking immunoprecipitation of the Argonaute protein in a panel of PCa cell lines modeling different stages of PCa progression. Using this comprehensive catalogue of miRNA targets, we analyzed miRNA targeting on known drivers of PCa and examined tissue-specific and stage-specific pathway targeting by miRNAs. We found that androgen receptor is the most frequently targeted PCa oncogene and that miR-148a targets the largest number of known PCa drivers. Globally, tissue-specific and stage-specific changes in miRNA targeting are driven by homeostatic response to active oncogenic pathways. Our findings indicate that, even in advanced PCa, the miRNA pool adapts to regulate continuing alterations in the cancer genome to balance oncogenic molecular changes. These findings are important because they are the first to globally characterize miRNA changes in PCa and demonstrate how the miRNA target spectrum responds to staged tumorigenesis.
LinkOut: [PMID: 27292025]
CLIP-seq Support 1 for dataset GSM545213
Method / RBP PAR-CLIP / AGO2
Cell line / Condition HEK293 / Control
Location of target site ENST00000274629.4 | 3UTR | CUUGGGUUUGAAUCCUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM545215
Method / RBP PAR-CLIP / AGO4
Cell line / Condition HEK293 / Control
Location of target site ENST00000274629.4 | 3UTR | UCUUGGGUUUGAAUCCUGUCUCU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 20371350 / GSE21578
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset SRR359787
Method / RBP PAR-CLIP / AGO2
Cell line / Condition hESCs (WA-09) / 4-thiouridine, RNase T1
Location of target site ENST00000274629.4 | 3UTR | AGAUGCAGAUCUUGGGUUUGAAUCCUGUCUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 22012620 / SRX103431
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM1065668
Method / RBP PAR-CLIP / AGO1
Cell line / Condition HEK293 / 4-thiouridine, ML_MM_7
Location of target site ENST00000274629.4 | 3UTR | GGUUUGAAUCCUGUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23446348 / GSE43573
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
75 hsa-miR-6715b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT080245 SMAD4 SMAD family member 4 2 2
MIRT142553 TNRC6A trinucleotide repeat containing 6A 2 4
MIRT384667 MARCH9 membrane associated ring-CH-type finger 9 2 2
MIRT446660 MXI1 MAX interactor 1, dimerization protein 2 4
MIRT463754 YTHDC1 YTH domain containing 1 2 2
MIRT476873 FEM1B fem-1 homolog B 2 2
MIRT478429 DAZAP2 DAZ associated protein 2 2 2
MIRT495237 SPRY3 sprouty RTK signaling antagonist 3 2 4
MIRT495945 ANKRD52 ankyrin repeat domain 52 2 2
MIRT497116 TYW3 tRNA-yW synthesizing protein 3 homolog 2 2
MIRT497344 RPP25L ribonuclease P/MRP subunit p25 like 2 2
MIRT498133 KLHL3 kelch like family member 3 2 2
MIRT502306 GNA13 G protein subunit alpha 13 2 2
MIRT510854 RAN RAN, member RAS oncogene family 2 8
MIRT513323 KCNMB1 potassium calcium-activated channel subfamily M regulatory beta subunit 1 2 6
MIRT523995 DTX4 deltex E3 ubiquitin ligase 4 2 4
MIRT525837 FAR2 fatty acyl-CoA reductase 2 2 2
MIRT527506 MYD88 myeloid differentiation primary response 88 2 2
MIRT527668 CIT citron rho-interacting serine/threonine kinase 2 2
MIRT527996 NDNF neuron derived neurotrophic factor 2 2
MIRT528052 MRS2 MRS2, magnesium transporter 2 2
MIRT528257 GPRIN2 G protein regulated inducer of neurite outgrowth 2 2 2
MIRT529401 ICK intestinal cell kinase 2 2
MIRT529558 SNRNP48 small nuclear ribonucleoprotein U11/U12 subunit 48 2 2
MIRT530323 TNFRSF10D TNF receptor superfamily member 10d 2 2
MIRT532230 KLF2 Kruppel like factor 2 2 2
MIRT534220 SLC35G1 solute carrier family 35 member G1 2 2
MIRT536195 MAML2 mastermind like transcriptional coactivator 2 2 2
MIRT538093 DEPDC1 DEP domain containing 1 2 2
MIRT543159 GTF2A1 general transcription factor IIA subunit 1 2 2
MIRT545840 ZNF264 zinc finger protein 264 2 4
MIRT546401 SRP9 signal recognition particle 9 2 2
MIRT550643 SLC39A7 solute carrier family 39 member 7 2 2
MIRT558648 CNKSR3 CNKSR family member 3 2 2
MIRT562208 HMGN2 high mobility group nucleosomal binding domain 2 2 2
MIRT562886 NACA2 nascent polypeptide associated complex alpha subunit 2 2 2
MIRT566786 MKL2 MKL1/myocardin like 2 2 2
MIRT567508 FOXC1 forkhead box C1 2 2
MIRT570235 CPNE5 copine 5 2 2
MIRT576823 Tgfbr3 transforming growth factor, beta receptor III 2 2
MIRT610704 GPX6 glutathione peroxidase 6 2 2
MIRT611141 CRISP1 cysteine rich secretory protein 1 2 4
MIRT618100 PPP5D1 PPP5 tetratricopeptide repeat domain containing 1 2 2
MIRT619086 OMA1 OMA1 zinc metallopeptidase 2 2
MIRT619716 FCF1 FCF1, rRNA-processing protein 2 2
MIRT620953 POLR2D RNA polymerase II subunit D 2 2
MIRT624645 ASXL3 additional sex combs like 3, transcriptional regulator 2 2
MIRT624655 ARMC10 armadillo repeat containing 10 2 2
MIRT630778 KLHL42 kelch like family member 42 2 4
MIRT635596 ATP9A ATPase phospholipid transporting 9A (putative) 2 2
MIRT637122 MKX mohawk homeobox 2 2
MIRT645527 ZWINT ZW10 interacting kinetochore protein 2 2
MIRT647249 PTGDR2 prostaglandin D2 receptor 2 2 2
MIRT651508 WNT9A Wnt family member 9A 2 2
MIRT658721 ELK4 ELK4, ETS transcription factor 2 2
MIRT658804 EFNA5 ephrin A5 2 2
MIRT659319 CSRNP3 cysteine and serine rich nuclear protein 3 2 2
MIRT664605 UGT3A1 UDP glycosyltransferase family 3 member A1 2 2
MIRT666047 STON2 stonin 2 2 2
MIRT672228 PDE7B phosphodiesterase 7B 2 2
MIRT683243 WFDC6 WAP four-disulfide core domain 6 2 2
MIRT686565 TPM3 tropomyosin 3 2 2
MIRT697560 ZBTB10 zinc finger and BTB domain containing 10 2 2
MIRT699294 SLC5A3 solute carrier family 5 member 3 2 2
MIRT707134 TRA2B transformer 2 beta homolog 2 2
MIRT712255 PTPRN2 protein tyrosine phosphatase, receptor type N2 2 2
MIRT713896 IGF2R insulin like growth factor 2 receptor 2 2
MIRT716186 UBN2 ubinuclein 2 2 2
MIRT716525 KSR2 kinase suppressor of ras 2 2 2
MIRT716968 CNOT6 CCR4-NOT transcription complex subunit 6 2 2
MIRT717154 LRRC3C leucine rich repeat containing 3C 2 2
MIRT718669 LSM10 LSM10, U7 small nuclear RNA associated 2 2
MIRT722100 PTBP3 polypyrimidine tract binding protein 3 2 2
MIRT723569 GEMIN4 gem nuclear organelle associated protein 4 2 2
MIRT724703 CRAMP1L cramped chromatin regulator homolog 1 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-6715b-3p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-6715b-3p Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)

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